18,110 results on '"atherosclerotic plaque"'
Search Results
2. Calculation of calcification volume and its effect on narrowing of cardiac blood vessels extracted from computed tomography angiography (CTA) images based on computer-aided diagnosis (CAD).
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Sari, Nur Endah, Almira, Levina, Lubis, Lukmanda Evan, Prajitno, Prawito, and Soejoko, Djarwani Soeharso
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COMPUTER-aided diagnosis , *COMPUTED tomography , *ARTERIAL occlusions , *ATHEROSCLEROTIC plaque , *CARDIAC imaging - Abstract
Use of Computer-Aided Diagnosis (CAD) on contrast and non-contrast Computed Tomography Angiography (CTA) cardiac images, to determine the location and calculate the volume of calcifications and study their effects on cardiac vascular constriction. Thresholding segmentation for plaque and arterial extraction, Segments Statistics for volume calculation, Extract Centerline and Centerline Metrics for calculating radius and length from 20 contrast and non-contrast CTA cardiac image datasets patients using Volume Viewer software from GE Healthcare and 3D-Slicer with Vascular Modelling Toolkit (VMTK) module. Number of arterial branches detected by calcification in Left Main (LM), Left Anterior Descending (LAD), Left Circumflex (LCX), and Right Coronary Artery (RCA), were 2, 19, 4, and 7 respectively. The largest volume of calcification detected in LM, LAD, LCX and RCA were 34.1 mm3, 22.9 mm3, 31.9 mm3, and 283.3 mm3, respectively. The maximum percentage of LM, LAD, LCX, and RCA artery closure was 76.5%; 83.8%; 67.4% and 90.5%, and the minimum value is 9.8%; 4.2%; 7.5% and 8.1%. Based on the Spearman statistical test, a strong correlation was obtained between the Agatston score and the largest calcification volume with an R-value of 0.774. Therefore, information on calcification volume and percentage of arterial occlusion should be used as a companion to the Agatston score, which has been used as the main parameter in the diagnosis of calcification. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Detection of Coronary Artery Calcifications by Whole Blood Transcriptome Analyzed by Artificial InTelligence Algorithms (CAC-TRAIT)
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Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia and Santiago Gabriel Miriuka, Co-Founder & CEO
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- 2024
4. Reduced Radiation Dose and IR-impact on Coronary Calcium Quantification
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Harold Litt, MD, PhD, Associate Professor of Radiology
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- 2024
5. Whole Blood Transcriptomic Signal According to Coronary Atherosclerotic Plaque Burden Assessed by CT Angiography (CORPLAQ-TRAIT)
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Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
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- 2024
6. MiRNA-132/212 encapsulated by adipose tissue-derived exosomes worsen atherosclerosis progression.
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Guo, Bei, Zhuang, Tong-Tian, Li, Chang-Chun, Li, Fuxingzi, Shan, Su-Kang, Zheng, Ming-Hui, Xu, Qiu-Shuang, Wang, Yi, Lei, Li-Min, Tang, Ke-Xin, Ouyang, Wenlu, Duan, Jia-Yue, Wu, Yun-Yun, Cao, Ye-Chi, Ullah, Muhammad Hasnain Ehsan, Zhou, Zhi-Ang, Lin, Xiao, Wu, Feng, Xu, Feng, and Liao, Xiao-Bo
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PLATELET-derived growth factor , *PTEN protein , *VASCULAR smooth muscle , *ATHEROSCLEROTIC plaque , *ADIPOSE tissues - Abstract
Background: Visceral adipose tissue in individuals with obesity is an independent cardiovascular risk indicator. However, it remains unclear whether adipose tissue influences common cardiovascular diseases, such as atherosclerosis, through its secreted exosomes. Methods: The exosomes secreted by adipose tissue from diet-induced obesity mice were isolated to examine their impact on the progression of atherosclerosis and the associated mechanism. Endothelial apoptosis and the proliferation and migration of vascular smooth muscle cells (VSMCs) within the atherosclerotic plaque were evaluated. Statistical significance was analyzed using GraphPad Prism 9.0 with appropriate statistical tests. Results: We demonstrate that adipose tissue-derived exosomes (AT-EX) exacerbate atherosclerosis progression by promoting endothelial apoptosis, proliferation, and migration of VSMCs within the plaque in vivo. MicroRNA-132/212 (miR-132/212) was detected within AT-EX cargo. Mechanistically, miR-132/212-enriched AT-EX exacerbates palmitate acid-induced endothelial apoptosis via targeting G protein subunit alpha 12 and enhances platelet-derived growth factor type BB-induced VSMC proliferation and migration by targeting phosphatase and tensin homolog in vitro. Importantly, melatonin decreases exosomal miR-132/212 levels, thereby mitigating the pro-atherosclerotic impact of AT-EX. Conclusion: These data uncover the pathological mechanism by which adipose tissue-derived exosomes regulate the progression of atherosclerosis and identify miR-132/212 as potential diagnostic and therapeutic targets for atherosclerosis. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Rescue therapy of early neurological deterioration in lacunar stroke.
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Park, Soo-Hyun, Kim, Jonguk, Yoon, Cindy W., Park, Hee-Kwon, and Rha, Joung-Ho
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STROKE patients , *CLINICAL deterioration , *AGE differences , *LACUNAR stroke , *PHENYLEPHRINE , *ATHEROSCLEROTIC plaque - Abstract
Background: Early neurological deterioration (END) occurs in many patients with acute ischemic stroke due to a variety of causes. Although pharmacologically induced hypertension (PIH) and anticoagulants have been investigated in several clinical trials for the treatment of END, the efficacy and safety of these treatments remain unclear. Here, we investigated whether PIH or anticoagulation is better as a rescue therapy for the progression of END in patients with lacunar stroke. Methods: This study included patients with lacunar stroke who received rescue therapy with END within 3 days of symptom onset between April 2014 and August 2021. In the PIH group, phenylephrine was administered intravenously for 24 h and slowly tapered when symptoms improved or after 5 days of PIH. In the anticoagulation group, argatroban was administered continuously intravenously for 2 days and twice daily for next 5 days. We compared END recovery, defined as improvement in NIHSS from baseline, excellent outcomes (0 or 1 mRS at 3 months), and safety profile. Results: Among the 4818 patients with the lacunar stroke, END occurred in 147 patients. Seventy-nine patients with END received PIH (46.9%) and 68 patients (46.3%) received anticoagulation therapy. There was no significant difference in age (P = 0.82) and sex (P = 0.87) between the two groups. Compared to the anticoagulation group, the PIH group had a higher incidence of END recovery (77.2% vs. 51.5%, P < 0.01) and excellent outcomes (34.2% vs. 16.2%, P = 0.04). PIH was associated with END (HR 2.49; 95% CI 1.06–5.81, P = 0.04). PIH remained associated with END recovery (adjusted HR 3.91; 95% CI 1.19–12.90, P = 0.02). Safety outcomes, like hemorrhagic conversion and mortality, were not significantly different between the two groups. Conclusions: As a rescue therapy for the progression of END in lacunar stroke patients, PIH with phenylephrine was more effective with similar safety compared to anticoagulation with argatroban. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Subclinical atherosclerosis burden in carotid and femoral territories in HIV subjects: relationships with HIV and non-HIV related factors.
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Ferrer, Pedro, López, Laura, Pérez, Juncal, Cabello, Noemi, Núñez, María José, Sagastagoitia, Iñigo, Cotarelo, Manuel, de Isla, Leopoldo Pérez, and Estrada, Vicente
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HIV infections , *HIV-positive persons , *CARDIOVASCULAR diseases , *FEMORAL artery , *UNIVARIATE analysis , *ATHEROSCLEROTIC plaque - Abstract
Background: Cardiovascular disease is a major cause of morbidity in an aging HIV population. However, risk estimation with the most frequent equations usually classifies HIV patients as having a low or moderate risk. Several studies have described a very high prevalence of subclinical atherosclerosis in a middle-aged, non-HIV population. There is insufficient body of knowledge to understand if this is the case in people living with HIV (PLWH). We aim to calculate the proportion of patients with subclinical atherosclerosis in a single site cohort of HIV-infected subjects. Methods: We have analyzed chronically HIV infected adults (≥ 18 years) who were on active follow-up in an HIV unit specialized in the care of cardiovascular health. The most recent clinical visit and vascular ultrasonography were used to assess the objectives of our research. Our primary objective was to describe the proportion of participants with subclinical atherosclerosis (focal protrusion into the lumen > 0.5 mm or > 50% of the surrounding IMT or a diffuse thickness > 1.5 mm) in a single site cohort of PLWH. Carotid and iliofemoral territories were evaluated. As a secondary objective we have run a multivariate analysis to determine which HIV and non-HIV factors might be related with the presence of atherosclerotic plaques. Findings We included a total of 463 participants between November 2017 to October 2019. Subjects were predominantly male (84.2%) with a mean age of 48.8 years (SD 10.7). Hypercholesterolemia (36%) was the most prevalent comorbidity followed by Hypertension (18%) and Hypertriglyceridemia (16%). Mean duration of HIV infection is 12.3 years. Overall, participants had been receiving cART for a median of 9.5 years. Subclinical atherosclerosis was found in 197 subjects (42.5%; CI 95% [38.0–47.2]). The disease was found more frequently in the femoral arteries (37.8%) than in the carotid vascular bed (18.6%). Despite some HIV factors correlated with the presence of plaques in a univariate analysis (e.g., time with HIV-1 RNA > 50 copies/mL or time from HIV diagnosis), the only two explanatory factors that remained associated with the presence of atherosclerotic plaques in the multivariate analysis were smoking (OR 5.47, 95% CI 3.36 – 8.90) and age (OR 1.13, 95%CI 1.10 – 1.16). Interpretation We have found a very high prevalence of subclinical atherosclerosis among our cohort of PLWH. Despite having analyzed several HIV factors, age and smoking have been found to be the only factors associated with the development of atherosclerotic plaques. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1.
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Wu, Yichen, Chen, Zilong, Zheng, Zhe, Li, Xiaoguang, Shu, Jiangcheng, Mao, Ruiqi, An, Jie, Fan, Siyuan, Luo, Ruijie, Guo, Yi, Xu, Wenjing, Liang, Minglu, Huang, Kai, and Wang, Cheng
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VASCULAR smooth muscle , *VASCULAR remodeling , *ATHEROSCLEROTIC plaque , *CAROTID artery , *ARTERIAL stenosis - Abstract
Background: Dysregulation of vascular homeostasis can induce cardiovascular diseases and increase global mortality rates. Although lineage tracing studies have confirmed the pivotal role of modulated vascular smooth muscle cells (VSMCs) in the progression of pathological vascular remodeling, the underlying mechanisms are still unclear. Methods: The expression of Tudor-SN was determined in VSMCs of artery stenosis, PDGF-BB-treated VSMCs and atherosclerotic plaque. Loss- and gain-of-function approaches were used to explore the role of Tudor-SN in the modulation of VSMCs phenotype both in vivo and in vitro. Results: In this study, we demonstrate that Tudor-SN expression is significantly elevated in injury-induced arteries, atherosclerotic plaques, and PDGF-BB-stimulated VSMCs. Tudor-SN deficiency attenuates, but overexpression aggravates the synthetic phenotypic switching of VSMCs and pathological vascular remodeling. Loss of Tudor-SN also reduces atherosclerotic plaque formation and increases plaque stability. Mechanistically, PTEN, the major regulator of the MAPK and PI3K-AKT signaling pathways, plays a vital role in Tudor-SN-mediated regulation on proliferation and migration of VSMCs. Tudor-SN facilitates the polyubiquitination and degradation of PTEN via NEDD4-1, thus exacerbating vascular remodeling under pathological conditions. BpV (HOpic), a specific inhibitor of PTEN, not only counteracts the protective effect of Tudor-SN deficiency on proliferation and migration of VSMCs, but also abrogates the negative effect of carotid artery injury-induced vascular remodeling in mice. Conclusions: Our findings reveal that Tudor-SN deficiency significantly ameliorated pathological vascular remodeling by reducing NEDD4-1-dependent PTEN polyubiquitination, suggesting that Tudor-SN may be a novel target for preventing vascular diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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10. 血清ANXA2、ADAMTS4水平与短暂性脑缺血 发作患者颈动脉斑块性质的关系.
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钱静, 张婷婷, and 陈露
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Objective To investigate the relationships between serum levels of membrane-bound protein A2 (ANXA2), a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS4) and character of carotid plaque in patients with transient ischemic attack (TIA) . Methods Totally 156 patients with TIA (observation group) were selected for carotid artery vascular ultrasound, and 78 healthy volunteers undergoing physical examination during the same period (control group) were selected. The peripheral venous blood was collected from all the study subjects, centrifuged and retained for serum, and serum ANXA2 and ADAMTS4 were detected by ELISA. Patients with TIA were admitted to the hospital and underwent carotid vascular ultrasound to assess carotid plaque formation and stability by two experienced imaging physicians. Risk factors for carotid plaque instability in TIA patients were analyzed using multifactorial Logistic regression model. The predictive value of serum ANXA2 and ADAMTS4 levels for carotid plaque instability in TIA patients was analyzed using the receiver operating characteristic (ROC) curve. Results The serum ANXA2 and ADAMTS4 levels of the observation group were higher than those of the control group (t=16. 636, 25. 358, both P<0. 05) . Carotid artery vascular ultrasound examination revealed that there were 76 cases of carotid plaque instability and 80 cases of carotid plaque stability or no carotid plaque in TIA patients, and the incidence of carotid plaque instability was 48. 72% (76/156) . Univariate analysis revealed that carotid plaque instability in TIA patients was associated with age, smoking, alcohol consumption, comorbid hypertension, stroke risk, and serum ANXA2 and ADAMTS4 levels (all P<0. 05) ; multifactorial Logistic regression analysis showed that smoking, alcohol consumption, increased risk of stroke, and elevated serum ANXA2 and ADAMTS4 levels were the independent risk factors for carotid plaque instability (all P<0. 05) . ROC curve analysis showed that the AUC of serum ANXA2 and ADAMTS4 levels alone and in combination in predicting carotid plaque instability in TIA patients was 0. 787, 0. 780, and 0. 874, respectively, and the AUC of serum ANXA2 and ADAMTS4 levels in predicting carotid plaque instability in TIA patients was greater than that of either alone (Z=3. 151, 3. 326, both P<0. 05) . Conclusion Serum ANXA2 and ADAMTS4 levels increased in patients with TIA, and the elevated levels of them are independent risk factors for carotid plaque instability; serum ANXA2 and ADAMTS4 levels have a certain predictive value for carotid plaque instability in patients with TIA, and the predictive value of them combined is higher. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Association of cardiovascular risk factors and intraplaque neovascularization in symptomatic carotid plaque.
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Zehao Liu, Lianlian Zhang, Bing Sun, and Yasuo Ding
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CARDIOVASCULAR diseases risk factors ,CONTRAST-enhanced ultrasound ,ATHEROSCLEROTIC plaque ,CAROTID artery stenosis ,NEOVASCULARIZATION - Abstract
Background and purpose: Cardiovascular risk factors are known to contribute to the formation of atherosclerotic plaques, which can result in carotid stenosis. However, the extent to which these factors are associated with intraplaque neovascularization, a key indicator of plaque vulnerability, remains unclear. To investigate this relationship, a study was conducted utilizing contrast-enhanced ultrasound (CEUS) to assess intraplaque neovascularization in symptomatic patients. Methods: A cohort of 157 symptomatic patients underwent evaluation using Contrast-Enhanced Ultrasound (CEUS) imaging to assess carotid intraplaque neovascularization, which was quantified based on the degree of plaque enhancement. The collected data encompassed baseline patient characteristics, results from biochemical examinations, cardiovascular risk factors, and medication usage history. Regression analyses were conducted to elucidate the relationship between carotid plaque neovascularization and various cardiovascular risk factors. Results: Patients with intraplaque neovascularization were more prone to have diabetes mellitus (OR 3.81, 95% CI 1.94-7.46, p < 0.001), dyslipidemia (OR 2.36, 95% CI 1.22-4.55, p = 0.011) and hypertension (OR 2.92, 95% CI 1.50-5.71, p = 0.002). Smoking increased the risk of having intraplaque neovascularization (OR 2.25, 95% CI 1.12-4.54, p = 0.023). Treatment with statins was significantly lower in patients with intraplaque neovascularization (OR 0.37, 95% CI 0.19-0.72, p = 0.003). In the multivariate analysis, diabetes mellitus (OR 3.27, 95% CI 1.10-9.78, p = 0.034) was independently related to the presence of intraplaque neovascularization. Meanwhile, compared to the patients in the first tertile of serum glucose (< 6.20 mmol/L), the patients in the third tertile (> 13.35 mmol/L) had the most significance of intraplaque neovascularization (OR 5.55, 95% CI 1.85-16.66, p = 0.002). Conclusion: The findings indicated that diabetes mellitus is a significant cardiovascular risk factor that is strongly associated with carotid intraplaque neovascularization. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Deficiency of 5‐HT2B receptors alleviates atherosclerosis by regulating macrophage phenotype through inhibiting interferon signalling.
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Liu, Yahan, Wang, Zhipeng, Fang, Li, Xu, Yaohua, Zhao, Beilei, Kang, Xuya, Zhao, Yanqing, Han, Jintao, Zhang, Yan, Dong, Erdan, and Wang, Nanping
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PERITONEAL macrophages , *ATHEROSCLEROTIC plaque , *CORONARY artery disease , *CARDIOVASCULAR diseases , *ATHEROSCLEROSIS - Abstract
Background and Purpose Experimental Approach Key Results Conclusion and Implications Elevated levels of 5‐HT have been correlated with coronary artery disease and cardiac events, suggesting 5‐HT is a potential novel factor in the development of atherosclerotic cardiovascular disease. However, the underlying pathological mechanisms of the 5‐HT system in atherosclerosis remain unclear. The 5‐HT2B receptor (5‐HT2BR), which establishes a positive feedback loop with 5‐HT, has been identified as a contributor to pathophysiological processes in various vascular disorders. In this study, we investigated the immunological impact of 5‐HT2BR in atherosclerosis‐prone apolipoprotein E‐deficient (ApoE−/−) mice.Plasma levels of 5‐HT were measured in mice using an ELISA kit. Atherosclerotic plaque formation, macrophage infiltration and inflammatory signalling were assessed in ApoE−/− mice by employing both pharmacological inhibition and genetic deficiency of 5‐HT2BR. Inflammasome activation was elucidated using peritoneal macrophages isolated from 5‐HT2BR‐deficient mice.An upregulation of 5‐HT2BR expression was observed in the aortas of ApoE−/− mice, exhibiting a strong correlation with the presence of macrophages in plaques. Atherosclerosis was attenuated in mice through pharmacological inhibition and genetic deficiency of 5‐HT2BR. Additionally, a significant reduction in atherosclerotic plaque size was achieved through bone marrow reconstitution with 5‐HT2BR‐deficient cells. 5‐HT2BR‐deficient macrophages showed attenuated interferon (IFN) signalling, NLRP3 inflammasome activation, and interleukin‐1β release. Moreover, macrophages primed with 5‐HT2BR deficiency displayed an anti‐inflammatory phenotype.These findings support the hypothesis that 5‐HT2BR in macrophages plays a causal role in the development of atherosclerosis, revealing a novel perspective for potential therapeutic strategies in atherosclerosis‐related diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Dihydromyricetin suppresses endothelial NLRP3 inflammasome activation and attenuates atherogenesis by promoting mitophagy.
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Hu, Qin, Li, Chengying, Zhang, Ting, Yi, Long, Shan, Yifan, Ma, Xiangyu, Cai, Tongjian, Ran, Li, Shen, Hui, and Li, Yafei
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SATURATED fatty acids , *ATHEROSCLEROTIC plaque , *VASCULAR endothelium , *NLRP3 protein , *HIGH-fat diet - Abstract
Background: NOD-like receptor protein 3 (NLRP3) inflammasome activation is indispensable for atherogenesis. Mitophagy has emerged as a potential strategy to counteract NLRP3 inflammasome activation triggered by impaired mitochondria. Our previous research has indicated that dihydromyricetin, a natural flavonoid, can mitigate NLRP3-mediated endothelial inflammation, suggesting its potential to treat atherosclerosis. However, the precise underlying mechanisms remain elusive. This study sought to investigate whether dihydromyricetin modulates endothelial mitophagy and inhibits NLRP3 inflammasome activation to alleviate atherogenesis, along with the specific mechanisms involved. Methods: Apolipoprotein E-deficient mice on a high-fat diet were administered daily oral gavages of dihydromyricetin for 14 weeks. Blood samples were procured to determine the serum lipid profiles and quantify proinflammatory cytokine concentrations. Aortas were harvested to evaluate atherosclerotic plaque formation and NLRP3 inflammasome activation. Concurrently, in human umbilical vein endothelial cells, Western blotting, flow cytometry, and quantitative real-time PCR were employed to elucidate the mechanistic role of mitophagy in the modulation of NLRP3 inflammasome activation by dihydromyricetin. Results: Dihydromyricetin administration significantly attenuated NLRP3 inflammasome activation and vascular inflammation in mice on a high-fat diet, thereby exerting a pronounced inhibitory effect on atherogenesis. Both in vivo and in vitro, dihydromyricetin treatment markedly enhanced mitophagy. This enhancement in mitophagy ameliorated the mitochondrial damage instigated by saturated fatty acids, thereby inhibiting the activation and nuclear translocation of NF-κB. Consequently, concomitant reductions in the transcript levels of NLRP3 and interleukin-1β (IL-1β), alongside decreased activation of NLRP3 inflammasome and IL-1β secretion, were discerned. Notably, the inhibitory effects of dihydromyricetin on the activation of NF-κB and subsequently the NLRP3 inflammasome were determined to be, at least in part, contingent upon its capacity to promote mitophagy. Conclusion: This study suggested that dihydromyricetin may function as a modulator to promote mitophagy, which in turn mitigates NF-κB activity and subsequent NLRP3 inflammasome activation, thereby conferring protection against atherosclerosis. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Antithrombotic Strategies for Patients With Peripheral Artery Disease: JACC Scientific Statement.
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Bonaca, Marc P., Barnes, Geoffrey D., Bauersachs, Rupert, Bessada, Youssef, Conte, Michael S., Dua, Anahita, Hess, Connie N., Serhal, Maya, Mena-Hurtado, Carlos, Weitz, Jeffrey I., and Beckman, Joshua A.
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PERIPHERAL vascular diseases , *FIBRINOLYTIC agents , *CORONARY artery disease , *ATHEROSCLEROTIC plaque , *DECISION making - Abstract
Patients with peripheral artery disease (PAD) experience major cardiovascular and limb events. Antithrombotic strategies including antiplatelets and anticoagulants remain a cornerstone of treatment and prevention. Recent trials have shown heterogeneity in the response to antithrombotic therapies in patients presenting primarily with PAD when compared to those presenting primarily with coronary artery disease. In addition, there is observed heterogeneity with regards to the effects of antiplatelets and anticoagulants with respect to different outcomes including cardiovascular and major adverse limb events. This, coupled with risks of bleeding, requires a patient-centered and holistic assessment of benefit-risk when selecting antithrombotic strategies for patients with PAD. A global multidisciplinary work group was convened to evaluate antithrombotic strategies in PAD and to summarize the current state of the art. Common clinical scenarios around antithrombotic decision making were provided. Finally, insights with regard to implementation future investigation were described. • Subjects with PAD are at high risk for plaque rupture and MI. • Intensive antithrombotic therapy should be considered for patients with PAD and high-risk for MACE and MALE. • Antithrombotic therapies in PAD should include risk stratification of ischemic and bleeding risk. [ABSTRACT FROM AUTHOR]
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- 2024
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15. 7th European Conference of Immunology, 1–4 September, 2024, Dublin, Ireland.
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BIOENGINEERING ,LIFE sciences ,MEDICAL sciences ,B cells ,FOLLICULAR dendritic cells ,ATHEROSCLEROTIC plaque ,AUTOIMMUNE diseases - Published
- 2024
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16. Lipid nanoparticles encapsulating curcumin for imaging and stabilization of vulnerable atherosclerotic plaques via phagocytic "eat-me" signals.
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Shi, Zhang, Huang, Jun, Chen, Chao, Zhang, Xuefeng, Ma, Zhiqiang, and Liu, Qi
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MAGNETIC resonance imaging , *ATHEROSCLEROTIC plaque , *IMAGE stabilization , *NANOMEDICINE , *CURCUMIN - Abstract
Curcumin potentiates the stabilization of atherosclerotic plaques by polarizing macrophages, but its non-specific targeting hinders its clinical application. We aim to harness multifunctional lipid nanoparticles (MLNPs) to facilitate the imaging and targeted delivery of curcumin specifically to inflammatory macrophages, counteracting vulnerable plaques and mitigating the risk of ischemic events. Cholesteryl-9-carboxynonanoate-(125I‑iron oxide nanoparticle/Curcumin)-lipid-coated nanoparticles [9-CCN-(125I-ION/Cur)-LNPs], namely MLNPs, are designed to carry hybrid imaging agents. These agents combine 125I-ION with lipids containing phagocytic 'eat-me' signals, inducing macrophages to engulf the MLNPs. Our research demonstrates that the designed MLNPs accurately accumulate at unstable plaques and are precisely visualized and highlighted by both SPECT and MRI. Furthermore, MLNPs achieve high efficiency in delivering 125I-ION and curcumin to macrophages, ultimately leading to significant M1-to-M2 macrophage polarization. These real-time imaging and polarization capabilities of plaques have immediate clinical applicability and may pave the way for novel therapies to stabilize unstable atherosclerotic plaques. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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17. Upper limb computed tomography (CT) angiography in the emergency department.
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Eleti, S., Hickman, S., and Wilson, A.
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MAGNETIC resonance angiography , *PERIPHERAL vascular diseases , *SOFT tissue injuries , *COMPUTED tomography , *ATHEROSCLEROTIC plaque , *DIGITAL subtraction angiography - Abstract
A range of abnormalities may acutely affect the upper limb (UL) extremity vasculature including trauma, peripheral vascular disease, and inflammatory conditions. Significant technical advances in computed tomography angiography (CTA) have led to the widespread adoption of this noninvasive modality for rapid evaluation of UL arterial abnormalities in the emergency department setting. A key advantage of CTA over traditional digital subtraction angiography (DSA) is the ability to evaluate concurrent osseous and soft tissue injuries. Accurate identification of pathology requires knowledge of normal UL arterial anatomy in addition to a high-quality study, which may be achieved with a robust CTA protocol. We describe the spectrum of imaging findings on upper limb CTA associated with various acute presentations. Traumatic vascular injuries may occur secondary to penetrating and blunt aetiologies appearing on CTA as contrast extravasation, pooling, pseudoaneurysm, occlusion, and arteriovenous fistula. Peripheral vascular disease manifests as atherosclerotic plaques with thready downstream opacification, and these may precipitate acute thromboembolic events. Inflammatory conditions affecting the UL vasculature includes large and small vessel vasculitides characterised by arterial mural thickening. The use of modalities, including ultrasound and magnetic resonance angiography (MRA), should be considered for further characterisation where appropriate. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Carotid and aortic plaque imaging using 3D gradient-echo imaging and the three-point Dixon method with improved motion-sensitized driven-equilibrium (iMSDE).
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Koori, Norikazu, Kamekawa, Hiroki, Naito, Takehiro, Takatsu, Yasuo, Fuse, Hiraku, Miyakawa, Shin, Yasue, Kenji, Takahashi, Masato, and Kurata, Kazuma
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THREE-dimensional imaging , *ATHEROSCLEROTIC plaque , *MANN Whitney U Test , *CAROTID artery , *BLOOD flow - Abstract
We devised a method that combines the 3D-Dixon-gradientecho (GRE) method with an improved motion-sensitized driven-equilibrium (iMSDE) to suppress blood flow signals. The purpose of this study was to evaluate the effectiveness of the new method we developed plaque imaging method (3D-Dixon-GRE with the iMSDE method). Retrospective cohort. Thirty-nine patients who underwent cervical plaque imaging. FIELD STRENGTH/SEQUENCE:3.0 T/3D-GRE. Signal intensities of the common carotid artery, aorta, plaque, muscle, and subcutaneous fat were measured through the VISTA and the 3D-Dixon-GRE with iMSDE methods, and each contrast was calculated. Used the Mann Whitney U test. P -values below 0.05 were considered statistically significant. Plaque and muscle contrast estimated through the VISTA method and 3D-Dixon-GRE with iMSDE method was 1.60 ± 0.96 and 2.04 ± 1.06, respectively, (P < 0.05). The contrast between the flow (common carotid artery and Aorta) and muscle according to the VISTA method and 3D-Dixon-GRE with iMSDE method was 0.24 ± 0.11 and 0.40 ± 0.12, respectively (P < 0.001). Finally, the mean contrast for subcutaneous fat and muscle at six locations was 3.05 ± 1.25 and 0.81 ± 0.23 for the VISTA method and 3D-Dixon-GRE with the iMSDE method, respectively (P < 0.001). Compared to the conventional method (VISTA), the 3D-Dixon-GRE with iMSDE method is preferable in relation to the fat suppression effect, but it is disadvantageous regarding blood flow signal suppression. Therefore, the 3D-Dixon-GRE with the iMSDE method could be considered useful for plaque imaging. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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19. ECM Microenvironment in Vascular Homeostasis: New Targets for Atherosclerosis.
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Zhang, Lu, Feng, Qianqian, and Kong, Wei
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ATHEROSCLEROTIC plaque , *EXTRACELLULAR matrix proteins , *VASCULAR remodeling , *EXTRACELLULAR matrix , *DRUG target - Abstract
Alterations in vascular extracellular matrix (ECM) components, interactions, and mechanical properties influence both the formation and stability of atherosclerotic plaques. This review discusses the contribution of the ECM microenvironment in vascular homeostasis and remodeling in atherosclerosis, highlighting Cartilage oligomeric matrix protein (COMP) and its degrading enzyme ADAMTS7 as examples, and proposes potential avenues for future research aimed at identifying novel therapeutic targets for atherosclerosis based on the ECM microenvironment. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Reproducibility of artificial intelligence–enabled plaque measurements between systolic and diastolic phases from coronary computed tomography angiography.
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Flores Tomasino, Guadalupe, Han, Donghee, Pimentel, Raymond, Paz, William, Liang, Juni, Cheng, Victor Y, Slomka, Piotr, Berman, Daniel S., and Dey, Damini
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HEART beat , *CORONARY angiography , *CORONARY artery disease , *COMPUTED tomography , *ATHEROSCLEROTIC plaque - Abstract
Objectives: Current coronary CT angiography (CTA) guidelines suggest both end-systolic and mid-diastolic phases of the cardiac cycle can be used for CTA image acquisition. However, whether differences in the phase of the cardiac cycle influence coronary plaque measurements is not known. We aim to explore the potential impact of cardiac phases on quantitative plaque assessment. Methods: We enrolled 39 consecutive patients (23 male, age 66.2 ± 11.5 years) who underwent CTA with dual-source CT with visually evident coronary atherosclerosis and with good image quality. End-systolic and mid- to late-diastolic phase images were reconstructed from the same CTA scan. Quantitative plaque and stenosis were analyzed in both systolic and diastolic images using artificial intelligence (AI)–enabled plaque analysis software (Autoplaque). Results: Overall, 186 lesions from 39 patients were analyzed. There were excellent agreement and correlation between systolic and diastolic images for all plaque volume measurements (Lin's concordance coefficient ranging from 0.97 to 0.99; R ranging from 0.96 to 0.98). There were no substantial intrascan differences per patient between systolic and diastolic phases (p > 0.05 for all) for total (1017.1 ± 712.9 mm3 vs. 1014.7 ± 696.2 mm3), non-calcified (861.5 ± 553.7 mm3 vs. 856.5 ± 528.7 mm3), calcified (155.7 ± 229.3 mm3 vs. 158.2 ± 232.4 mm3), and low-density non-calcified plaque volume (151.4 ± 106.1 mm3 vs. 151.5 ± 101.5 mm3) and diameter stenosis (42.5 ± 18.4% vs 41.3 ± 15.1%). Conclusion: Excellent agreement and no substantial differences were observed in AI-enabled quantitative plaque measurements on CTA in systolic and diastolic images. Following further validation, standardized plaque measurements can be performed from CTA in systolic or diastolic cardiac phase. Clinical relevance statement: Quantitative plaque assessment using artificial intelligence–enabled plaque analysis software can provide standardized plaque quantification, regardless of cardiac phase. Key Points: • The impact of different cardiac phases on coronary plaque measurements is unknown. • Plaque analysis using artificial intelligence–enabled software on systolic and diastolic CT angiography images shows excellent agreement. • Quantitative coronary artery plaque assessment can be performed regardless of cardiac phase. [ABSTRACT FROM AUTHOR]
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- 2024
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21. TRIM25-mediated XRCC1 ubiquitination accelerates atherosclerosis by inducing macrophage M1 polarization and programmed death.
- Author
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Wu, Hongxian, Gao, Wei, Ma, Yuanji, Zhong, Xin, Qian, Juying, Huang, Dong, and Ge, Junbo
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CHRONIC total occlusion , *CORONARY artery stenosis , *ARTERITIS , *ATHEROSCLEROTIC plaque , *VASCULAR remodeling - Abstract
Background: Macrophage-mediated cleaning up of dead cells is a crucial determinant in reducing coronary artery inflammation and maintaining vascular homeostasis. However, this process also leads to programmed death of macrophages. So far, the role of macrophage death in the progression of atherosclerosis remains controversial. Also, the underlying mechanism by which transcriptional regulation and reprogramming triggered by macrophage death pathways lead to changes in vascular inflammation and remodeling are still largely unknown. TRIM25-mediated RIG-I signaling plays a key role in regulation of macrophages fate, however the role of TRIM25 in macrophage death-mediated atherosclerotic progression remains unclear. This study aims to investigate the relationship between TRIM25 and macrophage death in atherosclerosis. Methods: A total of 34 blood samples of patients with coronary stent implantation, including chronic total occlusion (CTO) leisions (n = 14) or with more than 50% stenosis of a coronary artery but without CTO leisions (n = 20), were collected, and the serum level of TRIM25 was detected by ELISA. Apoe−/− mice with or without TRIM25 gene deletion were fed with the high-fat diet (HFD) for 12 weeks and the plaque areas, necrotic core size, aortic fibrosis and inflammation were investigated. TRIM25 wild-type and deficient macrophages were isolated, cultured and stimulated with ox-LDL, RNA-seq, real-time PCR, western blot and FACS experiments were used to screen and validate signaling pathways caused by TRIM25 deletion. Results: Downregulation of TRIM25 was observed in circulating blood of CTO patients and also in HFD-induced mouse aortas. After HFD for 12 weeks, TRIM25−/−ApoeE−/− mice developed smaller atherosclerotic plaques, less inflammation, lower collagen content and aortic fibrosis compared with TRIM25+/+ApoeE−/− mice. By RNA-seq and KEGG enrichment analysis, we revealed that deletion of TRIM25 mainly affected pyroptosis and necroptosis pathways in ox-LDL-induced macrophages, and the expressions of PARP1 and RIPK3, were significantly decreased in TRIM25 deficient macrophages. Overexpression of TRIM25 promoted M1 polarization and necroptosis of macrophages, while inhibition of PARP1 reversed this process. Further, we observed that XRCC1, a repairer of DNA damage, was significantly upregulated in TRIM25 deficient macrophages, inhibiting PARP1 activity and PARP1-mediated pro-inflammatory change, M1 polarization and necroptosis of macrophages. By contrast, TRIM25 overexpression mediated ubiquitination of XRCC1, and the inhibition of XRCC1 released PARP1, and activated macrophage M1 polarization and necroptosis, which accelerated aortic inflammation and atherosclerotic plaque progression. Conclusions: Our study has uncovered a crucial role of the TRIM25-XRCC1Ub-PARP1-RIPK3 axis in regulating macrophage death during atherosclerosis, and we highlight the potential therapeutic significance of macrophage reprogramming regulation in preventing the development of atherosclerosis. [ABSTRACT FROM AUTHOR]
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- 2024
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22. Plaque Evolution and Vessel Wall Remodeling of Intracranial Arteries: A Prospective, Longitudinal Vessel Wall MRI Study.
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Guo, Yin, Canton, Gador, Baylam Geleri, Duygu, Balu, Niranjan, Sun, Jie, Kharaji, Mona, Zanaty, Nadin, Wang, Xin, Zhang, Kaiyu, L. Tirschwell, David, Hatsukami, Thomas S., Yuan, Chun, and Mossa‐Basha, Mahmud
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CEREBRAL infarction ,ISCHEMIC stroke ,ATHEROSCLEROTIC plaque ,ODDS ratio ,LOGISTIC regression analysis - Abstract
Background: Progression of intracranial atherosclerotic disease (ICAD) is associated with ischemic stroke events and can be quantified with three‐dimensional (3D) intracranial vessel wall (IVW) MRI. However, longitudinal 3D IVW studies are limited and ICAD evolution remains relatively unknown. Purpose: To evaluate ICAD changes longitudinally and to characterize the imaging patterns of atherosclerotic plaque evolution. Study Type: Prospective. Population: 37 patients (69 ± 12 years old, 12 females) with angiography confirmed ICAD. Field Strength/Sequence: 3.0T/3D time‐of‐flight gradient echo sequence and T1‐ and proton density‐weighted fast spin echo sequences. Assessment: Each patient underwent baseline and 1‐year follow‐up IVW. Then, IVW data from both time points were jointly preprocessed using a multitime point, multicontrast, and multiplanar viewing workflow (known as MOCHA). Lumen and outer wall of plaques were traced and measured, and plaques were then categorized into progression, stable, and regression groups based on changes in plaque wall thickness. Patient demographic and clinical data were collected. Culprit plaques were identified based on cerebral ischemic infarcts. Statistical Tests: Generalized estimating equations‐based linear and logistic regressions were used to assess associations between vascular risk factors, medications, luminal stenosis, IVW plaque imaging features, and longitudinal changes. A two‐sided P‐value<0.05 was considered statistically significant. Results: Diabetes was significantly associated with ICAD progression, resulting in 6.6% decrease in lumen area and 6.7% increase in wall thickness at 1‐year follow‐up. After accounting for arterial segments, baseline contrast enhancement predicted plaque progression (odds ratio = 3.61). Culprit plaques experienced an average luminal expansion of 10.9% after 1 year. 74% of the plaques remained stable during follow‐up. The regression group (18 plaques) showed significant increase in minimum lumen area (from 7.4 to 8.3 mm2), while the progression group (13 plaques) showed significant decrease in minimum lumen area (from 5.4 to 4.3 mm2). Data Conclusion: Longitudinal 3D IVW showed ICAD remodeling on the lumen side. Culprit plaques demonstrated longitudinal luminal expansion compared with their non‐culprit counterparts. Baseline plaque contrast enhancement and diabetes mellitus were found to be significantly associated with ICAD changes. Evidence Level: 2 Technical Efficacy: Stage 3 [ABSTRACT FROM AUTHOR]
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- 2024
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23. Impact of Preeclampsia and Parity on Sex-based Discrepancies in Subclinical Carotid Atherosclerosis in Type 1 Diabetes.
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Perea, Verónica, Vinagre, Irene, Serés-Noriega, Tonet, Viñals, Clara, Mesa, Alex, Pané, Adriana, Milad, Camila, Esmatjes, Enric, Conget, Ignacio, Giménez, Marga, and Amor, Antonio J
- Subjects
CAROTID artery ultrasonography ,TYPE 1 diabetes ,ATHEROSCLEROTIC plaque ,CARDIOVASCULAR diseases ,PREECLAMPSIA ,CAROTID intima-media thickness - Abstract
Context The excess risk of fatal and nonfatal cardiovascular events is roughly twice as high in women than in men with type 1 diabetes. Objective To evaluate the impact of preeclampsia and parity on sex-based discrepancies in preclinical atherosclerosis and on the diagnostic performance of a cardiovascular risk scale. Design Cross-sectional study. Setting Single tertiary hospital. Patients A total of 728 people with type 1 diabetes (48.5% women) without cardiovascular disease and age ≥40 years, nephropathy, and/or ≥10 years of diabetes duration with another risk factor. Intervention Standardized carotid ultrasonography. Main Outcome Measures Carotid plaque determined by ultrasonography and cardiovascular risk estimated according to the Steno T1 Risk Engine (Steno-Risk). Results Nulliparous women and parous women without previous preeclampsia had a lower risk for carotid plaque than men (adjusted odds ratio:.48, 95% confidence interval [.28-.82]; adjusted odds ratio:.51 [.33-.79], respectively), without differences in the preeclampsia group. The prevalence of carotid plaque increased as the estimated cardiovascular risk increased in all subgroups except for preeclampsia group. The area under the curve of the Steno-Risk for identifying ≥2 carotid plaques was lower in the preeclampsia group (men:.7886; nulliparous women:.9026; women without preeclampsia:.8230; preeclampsia group:.7841; P between groups =.042). Neither the addition of parity nor preeclampsia in the Steno-Risk led to a statistically significant increase in the area under the curve. Conclusion The risk for carotid plaque in women compared with men decreased as exposure to obstetric factors diminished. However, the addition of these factors did not improve the prediction of the Steno-Risk. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Therapeutic potentials of peptide-derived nanoformulations in atherosclerosis: present status and future directions.
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Liu, Xue, Wang, Weijiao, Li, Qiang, Niu, Hongtao, and Zhang, Weili
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ATHEROSCLEROTIC plaque , *DRUG bioavailability , *ENDOTHELIAL cells , *STRUCTURAL stability , *TARGETED drug delivery - Abstract
Atherosclerosis is a severe cardiovascular disease followed by the accumulation of atherosclerotic plaques within the lumen of blood vessels resulting in reduced blood flow thus initiating a series of events. Conventional therapies for atherosclerosis encounter multiple challenges, especially difficulty in precisely concentrating in certain affected regions and the potential for unwanted side effects. Consequently, scientists are focused on developing nanoformulations for atherosclerosis diagnosis and therapy. Peptide-based nanomedicines improve conventional therapies by offering improved structural and therapeutic stability and enabling target-specific delivery. Their inherent biocompatibility and biodegradability additionally render them desirable materials intended for in vivo use. This review manuscript aims to provide an in-depth overview of peptide-based nanomedicines for atherosclerosis, focusing on targeted cells like endothelial cells, macrophages, and monocytes and their interaction with different plaque components. Moreover, the manuscript also highlights the latest progress in multimodal techniques and provides a comprehensive overview of limitations associated with their practical implementation. [ABSTRACT FROM AUTHOR]
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- 2024
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25. What have we learned from the REPRIEVE trial and where do we go from here?
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Grinspoon, Steven K., Lu, Michael T., Zanni, Markella V., Diggs, Marissa R., Chu, Sarah M., Ribaudo, Heather J., and Douglas, Pamela S.
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HIV infections , *MEDICAL societies , *LDL cholesterol , *DIGESTIVE system diseases , *MAJOR adverse cardiovascular events , *AIDS-related opportunistic infections , *ATHEROSCLEROTIC plaque - Abstract
The REPRIEVE trial investigated the use of statin therapy to prevent cardiovascular disease (CVD) in people with HIV. The trial involved over 7,700 participants from 12 countries and found that statin therapy reduced major adverse cardiovascular events by 36% over 5.6 years. This therapy was effective and safe, even for individuals without traditional risk factors. The results of the trial are applicable globally and emphasize the importance of considering factors such as sex, genetics, and local plaque biology when recommending CVD prevention therapy for people with HIV. The collaboration between various stakeholders was essential for the success of the study. [Extracted from the article]
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- 2024
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26. Aberrant mitochondrial DNA synthesis in macrophages exacerbates inflammation and atherosclerosis.
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Natarajan, Niranjana, Florentin, Jonathan, Johny, Ebin, Xiao, Hanxi, O'Neil, Scott Patrick, Lei, Liqun, Shen, Jixing, Ohayon, Lee, Johnson, Aaron R., Rao, Krithika, Li, Xiaoyun, Zhao, Yanwu, Zhang, Yingze, Tavakoli, Sina, Shiva, Sruti, Das, Jishnu, and Dutta, Partha
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CELL adhesion molecules ,DNA synthesis ,TRANSCRIPTION factors ,ATHEROSCLEROTIC plaque ,TRANSCRIPTOMES ,MITOCHONDRIAL DNA - Abstract
There is a large body of evidence that cellular metabolism governs inflammation, and that inflammation contributes to the progression of atherosclerosis. However, whether mitochondrial DNA synthesis affects macrophage function and atherosclerosis pathology is not fully understood. Here we show, by transcriptomic analyzes of plaque macrophages, spatial single cell transcriptomics of atherosclerotic plaques, and functional experiments, that mitochondrial DNA (mtDNA) synthesis in atherosclerotic plaque macrophages are triggered by vascular cell adhesion molecule 1 (VCAM-1) under inflammatory conditions in both humans and mice. Mechanistically, VCAM-1 activates C/EBPα, which binds to the promoters of key mitochondrial biogenesis genes - Cmpk2 and Pgc1a. Increased CMPK2 and PGC-1α expression triggers mtDNA synthesis, which activates STING-mediated inflammation. Consistently, atherosclerosis and inflammation are less severe in Apoe
−/− mice lacking Vcam1 in macrophages. Downregulation of macrophage-specific VCAM-1 in vivo leads to decreased expression of LYZ1 and FCOR, involved in STING signalling. Finally, VCAM-1 expression in human carotid plaque macrophages correlates with necrotic core area, mitochondrial volume, and oxidative damage to DNA. Collectively, our study highlights the importance of macrophage VCAM-1 in inflammation and atherogenesis pathology and proposes a self-acerbating pathway involving increased mtDNA synthesis. Macrophages and their metabolism are known to contribute to inflammation in the atherosclerotic plaques, but the underpinning molecular level regulatory processes are lesser known. Here authors show that under inflammatory conditions, macrophages express VCAM-1 within the atherosclerotic plaques, which leads to increased mitochondrial DNA synthesis via activation of the transcription factor C/EBPα, which in turn triggers inflammation by STING signalling. [ABSTRACT FROM AUTHOR]- Published
- 2024
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27. Cellular metabolism changes in atherosclerosis and the impact of comorbidities.
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Yusang Dai, Cruz Junho, Carolina Victoria, Schieren, Luisa, Wollenhaupt, Julia, Sluimer, Judith C., van der Vorst, Emiel P. C., and Noels, Heidi
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ATHEROSCLEROSIS ,VASCULAR endothelial cells ,VASCULAR smooth muscle ,ATHEROSCLEROTIC plaque ,METABOLISM ,DYSLIPIDEMIA ,OCULAR hypertension - Abstract
Cell activation and nutrient dysregulation are common consequences of atherosclerosis and its preceding risk factors, such as hypertension, dyslipidemia, and diabetes. These diseases may also impact cellular metabolism and consequently cell function, and the other way around, altered cellular metabolism can impact disease development and progression through altered cell function. Understanding the contribution of altered cellular metabolism to atherosclerosis and how cellular metabolism may be altered by co-morbidities and atherosclerosis risk factors could support the development of novel strategies to lower the risk of CVD. Therefore, we briefly review disease pathogenesis and the principles of cell metabolic pathways, before detailing changes in cellular metabolism in the context of atherosclerosis and comorbidities. In the hypoxic, inflammatory and hyperlipidemic milieu of the atherosclerotic plaque riddled with oxidative stress, metabolism shifts to increase anaerobic glycolysis, the pentose-phosphate pathway and amino acid use. We elaborate on metabolic changes for macrophages, neutrophils, vascular endothelial cells, vascular smooth muscle cells and lymphocytes in the context of atherosclerosis and its co-morbidities hypertension, dyslipidemia, and diabetes. Since causal relationships of specific key genes in a metabolic pathway can be cell type-specific and comorbidity-dependent, the impact of cell-specific metabolic changes must be thoroughly explored in vivo, with a focus on also systemic effects. When cell-specific treatments become feasible, this information will be crucial for determining the best metabolic intervention to improve atherosclerosis and its interplay with co-morbidities. [ABSTRACT FROM AUTHOR]
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- 2024
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28. Geometric changes and clinical risk factors from aortic arch to proximal internal carotid artery between normal subjects and moderate right carotid plaques.
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Hong An Ngo, Dac, Lee, Ui Yun, and Kwak, Hyo Sung
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ATHEROSCLEROTIC plaque , *THORACIC aorta , *CAROTID artery , *INTERNAL carotid artery , *CARDIOVASCULAR diseases risk factors , *GLOMERULAR filtration rate , *COMPUTED tomography - Abstract
The anatomical features spanning from the aortic arch to the proximal carotid artery and the associated cardiovascular risks might significantly influence the development of right carotid plaque. Our research aimed to compare these anatomical and risk factors between individuals with no carotid plaque and those with moderate right-side carotid plaque within a Korean cohort. We conducted a retrospective, cross-sectional analysis involving 413 participants, categorized into a normal group (n = 339) and a right moderate carotid plaque group (defined as > 50% stenosis based on NASCET criteria) (n = 74). We collected data on cardiovascular risk factors and conducted laboratory tests. A 3D model of the carotid artery was constructed using cranio-cervical computed tomography angiography (CTA) data through semi-automated software. Measurements taken on this 3D model included the common carotid artery (CCA), internal carotid artery (ICA), external carotid artery (ECA), and carotid artery bifurcation (CAB) in terms of maximal vascular diameter, sectional area, angles of carotid bifurcation and ICA, and carotid tortuosity. When compared with the normal group, individuals in the right moderate carotid plaque group exhibited smaller angles at the carotid bifurcation, larger CCA diameter and sectional area (p < 0.01), advanced age, and a higher incidence of hypertension, diabetes, and stroke history (p < 0.05), along with reduced glomerular filtration rate (GFR) (p < 0.001). Multivariate analysis revealed that the sectional area of the bifurcation, calcification of the aortic bulb, and GFR were independently associated with the presence of right moderate carotid plaque (p < 0.01). Statistical analyses disclosed significant differences in both clinical risk factors and geometric changes in the region extending from the aortic arch to the proximal carotid artery among subjects with right moderate carotid plaque when compared to those without. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Syndecan-1 as a predictor of vulnerable atherosclerotic plaques.
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Yan Qiu, Zhi Ouyang, Jian Zhong, Linlu Jin, Yixue Qin, and Ye Zeng
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ATHEROSCLEROTIC plaque ,SYNDECANS ,VASCULAR endothelial growth factors ,FOAM cells ,BIOMARKERS ,HIGH-fat diet - Abstract
Aims: Cardiovascular disease remains a major global health concern, with atherosclerosis (AS) being a significant contributor. Vulnerable plaques play a critical role in acute cardiovascular events. Syndecan-1 (SDC-1), a vital membrane proteoglycan in the vascular endothelial glycocalyx, is believed to be associated with plaque progression. However, its precise relationship with severity and vulnerability of atherosclerotic plaque remains unclear. This study aimed to investigate SDC-1 expression and its potential correlation with plaque vulnerability in ApoE
-/- atherosclerosis mouse model. Methods and results: Eight-week-old mice were induced into the AS model using a high-fat diet (HFD) and/or partial ligation of the left common carotid artery (PLCA), with a chow diet (CD) control group. After 16 weeks, plaques in the aortic root showed the following order: HFD + PLCA group > HFD group > CD + PLCA group > CD group. Immunohistochemistry revealed heightened accumulation of lipid/foam cells and CD68-labeled macrophages in the plaques, elevated vascular endothelial growth factor (VEGF), and matrix Metalloproteinase-9 (MMP-9) in the HFD + PLCA group's plaques, along with reduced collagen and α-SMA-labeled smooth muscle cells, resulting in the highest vulnerability index value. Immunohistofluorescence analysis of frozen plaque sections showed significantly higher SDC-1 expression in the AS mice group compared to the CD group, both positively correlated with plaque vulnerability. Serum analysis demonstrated elevated levels of SDC1, sphingosine 1-phosphate (S1P), and VEGF-A in the AS mice, all positively correlated with plaque vulnerability. Multivariate analysis identified SDC1 as an independent predictor of plaque vulnerability. Conclusion: This study enhances our understanding of plaque vulnerability mechanisms and presents SDC1 as a potential biomarker for atherosclerosis. These findings underscore the importance of addressing modifiable risk factors, such as diet and hemodynamics and suggest the utility of serum SDC1 as a valuable clinical marker. Ultimately, these insights may lead to more effective strategies in combating cardiovascular diseases and improving patient outcomes. [ABSTRACT FROM AUTHOR]- Published
- 2024
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30. Moving from lipids to leukocytes: inflammation and immune cells in atherosclerosis.
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Annink, Maxim E., Kraaijenhof, Jordan M., Stroes, Erik S. G., and Kroon, Jeffrey
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ATHEROSCLEROSIS ,VASCULAR endothelium ,LEUCOCYTES ,INFLAMMATION ,TREATMENT effectiveness ,ATHEROSCLEROTIC plaque - Abstract
Atherosclerotic cardiovascular disease (ASCVD) is the most important cause of morbidity and mortality worldwide. While it is traditionally attributed to lipid accumulation in the vascular endothelium, recent research has shown that plaque inflammation is an important additional driver of atherogenesis. Though clinical outcome trials utilizing anti-inflammatory agents have proven promising in terms of reducing ASCVD risk, it is imperative to identify novel actionable targets that are more specific to atherosclerosis to mitigate adverse effects associated with systemic immune suppression. To that end, this review explores the contributions of various immune cells from the innate and adaptive immune system in promoting and mitigating atherosclerosis by integrating findings from experimental studies, high-throughput multi-omics technologies, and epidemiological research. [ABSTRACT FROM AUTHOR]
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- 2024
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31. Catalpol inhibits HHcy-induced EndMT in endothelial cells by modulating ROS/NF-κB signaling.
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Wu, Chengyan, Li, Yuanhao, Liu, Shuangshuang, Wang, Libo, and Wang, Xuehui
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CELL transformation ,ENDOTHELIAL cells ,CELL morphology ,ATHEROSCLEROTIC plaque ,UMBILICAL veins - Abstract
Background: Hyperhomocysteinemia (HHcy) is an independent risk factor for atherosclerosis (AS). Endothelial mesenchymal transition (EndMT) refers to the process in which endothelial cells lose endothelial cell morphology and characteristic gene expression, and acquire phenotypic characteristics and gene expression related to mesenchymal cells. Numerous studies have confirmed that EndMT is involved in the formation of atherosclerosis. Catalpol is one of the active components of Rehmannia, which has antioxidant, anti-inflammatory, anti-tumor, neuroprotective and other biological activities. Studies have shown that catalpol can reduce atherosclerotic plaque induced by high sugar or fat. However, the effect of catalpol on HHCY-induced EndMT is unclear. Methods and results: In vitro HHcy-treated primary human umbilical vein endothelial cells (HUVECs) were used to construct a cell model, and the antioxidants N-acetylcysteine (NAC) and catalase alcohol were administered. In vivo C57BL/6N mice were given a diet fed with 4.4% high methionine chow to construct a HHcy mice model and were treated with catalpol. The results showed that hhcy could induce morphological transformation of endothelial cells into mesenchymal cells, increase intracellular ROS content, up-regulate α-SMA, N-cadherin, p-p65 protein expression, down-regulate VE-cadherin, CD31 protein expression, induce pathological changes of aortic root endothelium, and increase aortic endothelial ROS content. Catalpol reversed these hhcy induced outcomes. Conclusions: Catalpol inhibits HHcy-induced EndMT, and the underlying mechanism may be related to the ROS/NF-κB signaling pathway. Catalpol may be a potential drug for the treatment of HHcy-related AS. [ABSTRACT FROM AUTHOR]
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- 2024
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32. An Unsupervised Learning Tool for Plaque Tissue Characterization in Histopathological Images.
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Fraschini, Matteo, Castagnola, Massimo, Barberini, Luigi, Sanfilippo, Roberto, Coghe, Ferdinando, Didaci, Luca, Cau, Riccardo, Frongia, Claudio, Scartozzi, Mario, Saba, Luca, and Faa, Gavino
- Subjects
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TEXTURE analysis (Image processing) , *ATHEROSCLEROTIC plaque , *CAUSES of death , *PATHOLOGISTS , *STROKE - Abstract
Stroke is the second leading cause of death and a major cause of disability around the world, and the development of atherosclerotic plaques in the carotid arteries is generally considered the leading cause of severe cerebrovascular events. In recent years, new reports have reinforced the role of an accurate histopathological analysis of carotid plaques to perform the stratification of affected patients and proceed to the correct prevention of complications. This work proposes applying an unsupervised learning approach to analyze complex whole-slide images (WSIs) of atherosclerotic carotid plaques to allow a simple and fast examination of their most relevant features. All the code developed for the present analysis is freely available. The proposed method offers qualitative and quantitative tools to assist pathologists in examining the complexity of whole-slide images of carotid atherosclerotic plaques more effectively. Nevertheless, future studies using supervised methods should provide evidence of the correspondence between the clusters estimated using the proposed textural-based approach and the regions manually annotated by expert pathologists. [ABSTRACT FROM AUTHOR]
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- 2024
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33. Biomimetic Nanoparticles for the Diagnosis and Therapy of Atherosclerosis.
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Wang, Yan, Li, Yize, Lu, Yuqing, and Li, Jingjing
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ATHEROSCLEROTIC plaque , *CEREBRAL infarction , *CELL membranes , *MYOCARDIAL infarction , *CELL physiology - Abstract
Atherosclerosis (AS) is a chronic inflammation of blood vessels, which often has no obvious symptoms in the early stage of the disease, but when atherosclerotic plaques are formed, they often cause lumen blockage, and even plaque rupture leads to thrombosis, that is the essential factor of cardiovascular events, for example myocardial infarction, cerebral infarction, and renal atrophy. Therefore, it is considerably significant for the early recognition and precise therapy of plaque. Biomimetic nanoparticles (BNPs), especially those coated with cell membranes, can retain the biological function of cell membranes or cells, which has led to extensive research and application in the diagnosis and treatment of AS in recent years. In this review, we summarized the roles of various key cells in AS progression, the construction of biomimetic nanoparticles based on these key cells as well as their applications in AS diagnosis and therapy. Furthermore, we give a challenge and prospect of biomimetic nanoparticles in AS, hoping to elevate their application quality and the possibility of clinical translation. [ABSTRACT FROM AUTHOR]
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- 2024
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34. TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype.
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Hayderi, Assim, Zegeye, Mulugeta Melkie, Meydan, Sare, Sirsjö, Allan, Kumawat, Ashok Kumar, and Ljungberg, Liza U.
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CELL adhesion molecules , *TUMOR necrosis factors , *BASAL lamina , *GENE expression , *ATHEROSCLEROTIC plaque , *CELL adhesion - Abstract
Laminins are essential components of the basement membranes, expressed in a tissue- and cell-specific manner under physiological conditions. During inflammatory circumstances, such as atherosclerosis, alterations in laminin composition within vessels have been observed. Our study aimed to assess the influence of tumor necrosis factor-alpha (TNF), a proinflammatory cytokine abundantly found in atherosclerotic lesions, on endothelial laminin gene expression and the effects of laminin-332 (LN332) on endothelial cells' behavior. We also evaluated the expression of LN332-encoding genes in human carotid atherosclerotic plaques. Our findings demonstrate that TNF induces upregulation of LAMB3 and LAMC2, which, along with LAMA3, encode the LN332 isoform. Endothelial cells cultured on recombinant LN332 exhibit decreased claudin-5 expression and display a loosely connected phenotype, with an elevated expression of chemokines and leukocyte adhesion molecules, enhancing their attractiveness and adhesion to leukocytes in vitro. Furthermore, LAMB3 and LAMC2 are upregulated in human carotid plaques and show a positive correlation with TNF expression. In summary, TNF stimulates the expression of LN332-encoding genes in human endothelial cells and LN332 promotes an endothelial phenotype characterized by compromised junctional integrity and increased leukocyte interaction. These findings highlight the importance of basement membrane proteins for endothelial integrity and the potential role of LN332 in atherosclerosis. [ABSTRACT FROM AUTHOR]
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- 2024
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35. Atorvastatin-based nanoparticle-decorated glyceryl monostearate as a targeted drug carrier for inhibition of atherosclerosis lesions.
- Author
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Yadav, Sandeep Kumar, Karthik, Karri, Mahadevappa, Manjunatha, and Das, Soumen
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DRUG delivery systems , *DRUG carriers , *ATHEROSCLEROSIS , *ATHEROSCLEROTIC plaque , *TREATMENT effectiveness , *STAINS & staining (Microscopy) - Abstract
Macrophages play a significant role in various phases of a chronic atherosclerotic plaque of the arterial wall. Oxidized low-density lipoprotein (Ox-LDL) accumulation in the tunica intima of an artery causes endothelial dysfunction in atherosclerosis. Statins are utilized to treat atherosclerosis, but they carry numerous adverse effects and inadequate therapeutic effects. Herein, atorvastatin nanoparticles (ATR NPs) are designed for treating atherosclerosis and enhancing the prolonged therapeutic efficacy related to the treatment of atherosclerotic plaques. For the synthesis of the ATR NPs, we have used atorvastatin-decorated glyceryl monostearate (GMS) in conjunction with ultra-sonication techniques. The GMS loaded ATR NPs were verified with characterization using multiple techniques to study their physicochemical properties. The synthesized ATR NPs revealed excellent stability, greater drug encapsulation efficiency (76.84 ± 4.7), and a sustained drug release profile, making them suitable for drug delivery systems. Live/dead staining and flow cytometry analysis showed that ATR NPs prevented cellular apoptosis and improved foam cell survival. Confocal microscopy images of ATR NPs associated with rhodamine 6G (Rh-6G) drugs revealed that ATR NPs improved cell uptake and internalization through endocytosis pathways. Using Oil Red O (ORO) staining, ATR NPs increased cholesterol efflux from arterial walls and clearance of apoptotic cells. ATR NPs reduced plaque buildup in atherosclerotic apoE−/− mice after intravenous administration. These findings illustrate that ATR nanoparticles are more effective for treatment of atherosclerotic lesions. [ABSTRACT FROM AUTHOR]
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- 2024
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36. The biological applications of near-infrared optical nanomaterials in atherosclerosis.
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Shen, Lin, Bi, Yanran, Yu, Junchao, Zhong, Yi, Chen, Weiqian, Zhao, Zhongwei, Ding, Jiayi, Shu, Gaofeng, Chen, Minjiang, Lu, Chenying, and Ji, Jiansong
- Subjects
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CARDIOVASCULAR disease diagnosis , *ATHEROSCLEROTIC plaque , *NANOPARTICLES , *VASCULAR diseases , *ANGIOGRAPHY - Abstract
Purpose of review: Atherosclerosis, a highly pathogenic and lethal disease, is difficult to locate accurately via conventional imaging because of its scattered and deep lesions. However, second near-infrared (NIR-II) nanomaterials show great application potential in the tracing of atherosclerotic plaques due to their excellent penetration and angiographic capabilities. Recent findings: With the development of nanotechnology, among many nanomaterials available for the visual diagnosis and treatment of cardiovascular diseases, optical nanomaterials provide strong support for various biomedical applications because of their advantages, such as noninvasive, nondestructive and molecular component imaging. Among optical nanomaterials of different wavelengths, NIR-II-range (900 ~ 1700 nm) nanomaterials have been gradually applied in the visual diagnosis and treatment of atherosclerosis and other vascular diseases because of their deep biological tissue penetration and limited background interference. Summary: This review explored in detail the prospects and challenges of the biological imaging and clinical application of NIR-II nanomaterials in treating atherosclerosis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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37. Role of coronary computed tomography angiography to optimise percutaneous coronary intervention outcomes.
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Bouisset, Frederic, Ohashi, Hirofumi, Andreini, Daniele, and Collet, Carlos
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SINGLE-photon emission computed tomography ,CORONARY circulation ,INTRAVASCULAR ultrasonography ,CORONARY artery calcification ,CHRONIC total occlusion ,CORONARY artery surgery ,MYOCARDIAL perfusion imaging ,ATHEROSCLEROTIC plaque ,CHEST pain - Published
- 2024
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38. Prevalence and clinical implications of calcification in internal carotid artery stenosis: a retrospective study.
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Fu, Fengli, Liu, Xiaoli, Zhang, Rui, Zhang, Siran, Mao, Jianhua, Li, Yan, Wan, Shu, and Xu, Shanhu
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INTERNAL carotid artery , *ATHEROSCLEROTIC plaque , *COMPUTED tomography , *ISCHEMIC stroke ,CAROTID artery stenosis - Abstract
Background: Calcification is common in advanced atheromatous plaque, but its clinical significance remains unclear. This study aimed to assess the prevalence of plaque calcification in the moderate-to-severe internal carotid artery stenosis and investigate its relationship with ipsilateral ischemia. Methods: The retrospective study included 178 patients detected with proximal internal carotid artery (pICA) stenosis of ≥ 50% on multidetector computed tomography at Zhejiang Hospital from January 2019 to March 2023. Association between plaque calcification characteristics (calcification thickness, position, type, circumferential extent, calcium volume and calcium score) and ipsilateral cerebrovascular events was analyzed. Results: The 178 patients (mean age 71.24 ± 10.02 years, 79.78% males) had 224 stenosed pICAs overall. Plaque calcification was noted in 200/224 (89.29%) arteries. Calcification rates were higher in older age-groups. Calcification volume (r = 0.219, p < 0.001) and calcification score (r = 0.230, p < 0.001) were correlated with age. Ipsilateral ischemic events were significantly more common in the noncalcification group than in the calcification group (χ2 = 4.160, p = 0.041). The most common calcification type was positive rim sign calcification (87/200, 43.50%), followed by bulky calcification (66/200, 33.00%); both were significantly associated with ischemic events (χ2 = 10.448, p = 0.001 and χ2 = 4.552, p = 0.033, respectively). Calcification position, thickness, and circumferential extent, and calcification volume and score, were not associated with ischemic events. In multivariate analysis, positive rim signs (OR = 2.795, 95%CI 1.182–6.608, p = 0.019) was an independent predictor of ischemic events. Conclusions: Plaque calcification in proximal internal carotid artery is common, and prevalence increases with age. Calcification characteristics could be predictive of ipsilateral ischemic events. The positive rim sign within plaque is a high-risk factor for a future ischemic event. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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39. PIEZO1 targeting in macrophages boosts phagocytic activity and foam cell apoptosis in atherosclerosis.
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Pourteymour, Shirin, Fan, Jingxue, Majhi, Rakesh Kumar, Guo, Shuyuan, Sun, Xin, Huang, Zhen, Liu, Ying, Winter, Hanna, Bäcklund, Alexandra, Skenteris, Nikolaos-Taxiarchis, Chernogubova, Ekaterina, Werngren, Olivera, Li, Zhaolong, Skogsberg, Josefin, Li, Yuhuang, Matic, Ljubica, Hedin, Ulf, Maegdefessel, Lars, Ehrenborg, Ewa, and Tian, Ye
- Subjects
- *
FOAM cells , *ATHEROSCLEROTIC plaque , *REACTIVE oxygen species , *PHAGOCYTOSIS , *ATHEROSCLEROSIS - Abstract
The rising incidences of atherosclerosis have necessitated efforts to identify novel targets for therapeutic interventions. In the present study, we observed increased expression of the mechanosensitive calcium channel Piezo1 transcript in mouse and human atherosclerotic plaques, correlating with infiltration of PIEZO1-expressing macrophages. In vitro administration of Yoda1, a specific agonist for PIEZO1, led to increased foam cell apoptosis and enhanced phagocytosis by macrophages. Mechanistically, PIEZO1 activation resulted in intracellular F-actin rearrangement, elevated mitochondrial ROS levels and induction of mitochondrial fragmentation upon PIEZO1 activation, as well as increased expression of anti-inflammatory genes. In vivo, ApoE−/− mice treated with Yoda1 exhibited regression of atherosclerosis, enhanced stability of advanced lesions, reduced plaque size and necrotic core, increased collagen content, and reduced expression levels of inflammatory markers. Our findings propose PIEZO1 as a novel and potential therapeutic target in atherosclerosis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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40. Correlation between the TyG-BMI index and carotid plaque characteristics in middle-aged and elderly patients with acute myocardial infarction.
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Zhang, Lei-Guang, Li, Hui-Juan, Liu, Shuai, and Liu, Jie-Yun
- Subjects
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CAROTID artery ultrasonography , *MYOCARDIAL infarction , *ATHEROSCLEROTIC plaque , *OLDER patients , *LOGISTIC regression analysis - Abstract
The aim of this study is to investigate the correlation between the triglyceride-glucose-body mass index (TyG-BMI) and the characteristics of various carotid plaques in middle-aged and elderly patients with acute myocardial infarction (AMI).A retrospective study was conducted on 380 patients with AMI hospitalized in the Cardiology Department of Kaifeng Central Hospital. Based on carotid ultrasound results, patients were divided into the following two groups: the stable plaque group and the unstable plaque group. Additionally, a control group comprising 380 healthy individuals visiting the hospital’s physical examination center during the same timeframe was established. Fasting venous blood samples were collected from all participants to measure blood glucose and triglyceride. The baseline TyG-BMI index was calculated using the formula Ln [fasting triglyceride (mg/dL)×fasting blood glucose (mg/dL)/2]×BMI. The correlation between different plaque groups and the TyG-BMI index was analyzed.The TyG-BMI index was significantly higher in the unstable plaque group compared to the stable plaque group, with values of 252.81±29.99 and 201.92±28.72, respectively (
P = 0.034). Spearman’s correlation analysis showed a positive correlation between the instability of carotid plaques and the TyG-BMI index in patients with AMI (r = 0.521,P = 0.003). Logistic regression analysis indicated that the TyG-BMI index was an important risk factor for unstable carotid plaques in patients with AMI (OR = 2.691, 95% CI: 1.169–4.123).The findings of this study suggest that an elevated TyG-BMI index significantly increases the risk of unstable carotid plaques in patients with AMI, making it an important risk factor for carotid plaque instability. [ABSTRACT FROM AUTHOR]- Published
- 2024
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41. Molecular Imaging of Fibroblast Activation in Rabbit Atherosclerotic Plaques: a Preclinical PET/CT Study.
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Ji, Tianxiong, Zan, Chunfang, Li, Lina, Cao, Jianbo, Su, Yao, Wang, Hongliang, Wu, Zhifang, Yang, Min-Fu, Dou, Kefei, and Li, Sijin
- Subjects
- *
ATHEROSCLEROTIC plaque , *POSITRON emission tomography , *CAROTID intima-media thickness , *FIBROBLASTS , *RABBITS , *STAINS & staining (Microscopy) , *MYOCARDIAL infarction - Abstract
Aim: Atherosclerosis remains the pathological basis of myocardial infarction and ischemic stroke. Early and accurate identification of plauqes is crucial to improve clinical outcomes of atherosclerosis patients. Our study aims to evaluate the potential value of fibroblast activation protein inhibitor (FAPI)-04 PET/CT in identifying plaques via a preclinical rabbit model of atherosclerosis. Methods: New Zealand white rabbits were fed high-fat diet (HFD), and randomly divided into the model group injured by the balloon, and the sham group only with incisions. Ultrasound was performed to detect plaques, and FAPI-avid was determined through Al18F-NOTA-FAPI-04 PET/CT. Mean standardized uptake values (SUVmean) in lesions were compared, and biodistribution of Al18F-NOTA-FAPI-04 and target-to-background ratios (TBRs) were calculated. Histological staining was performed to display arterial plaques, and autoradiography (ARG) was employed to measure the in vitro intensity of Al18F-NOTA-FAPI-04. At last, the correlation among FAP levels, plaque area, SUVmean values and fibrous cap thickness was assessed. Results: The rabbit carotid and abdominal atherosclerosis model was established. Al18F-NOTA-FAPI-04 showed a higher uptake in carotid plaques (SUVmean 1.32 ± 0.11) and abdominal plaques (SUVmean 0.73 ± 0.13) compared to corresponding controls (SUVmean 1.07 ± 0.06; 0.46 ± 0.03) (P < 0.05). Biodistribution analysis of Al18F-NOTA-FAPI-04 revealed that the bigger plaques were delineated with higher TBRs. Pathological staining showed the formation of arterial plaques, and ARG staining exhibited a higher intensity of Al18F-NOTA-FAPI-04 in the bigger plaques. Lastly, plaque area was found to be positively correlated to FAP expression and SUVmean, while FAP expression was negatively correlated to fibrous cap thickness of plaques. Conclusions: We successfully achieve molecular imaging of fibroblast activation in atherosclerotic lesions of rabbits, suggesting Al18F-NOTA-FAPI-04 PET/CT may be a potentially valuable tool to identify plaques. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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42. Omics Science and Social Aspects in Detecting Biomarkers for Diagnosis, Risk Prediction, and Outcomes of Carotid Stenosis.
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Costa, Davide, Scalise, Enrica, Ielapi, Nicola, Bracale, Umberto Marcello, Faga, Teresa, Michael, Ashour, Andreucci, Michele, and Serra, Raffaele
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LIFE sciences , *CAROTID artery , *TRANSCRIPTOMES , *ATHEROSCLEROTIC plaque , *METABOLOMICS ,CAROTID artery stenosis - Abstract
Carotid stenosis is characterized by the progressive narrowing of the carotid arteries due to the formation of atherosclerotic plaque, which can lead to stroke and death as major complications. Numerous biomarkers allow for its study and characterization, particularly those related to "omics" sciences. Through the most common research databases, we report representative studies about carotid stenosis biomarkers based on genomics, transcriptomics, proteomics, and metabolomics in a narrative review. To establish a priority among studies based on their internal validity, we used a quality assessment tool, the Scale for the Assessment of Narrative Review Articles (SANRA). Genes, transcriptomes, proteins, and metabolites can diagnose the disease, define plaque connotations, predict consequences after revascularization interventions, and associate carotid stenosis with other patient comorbidities. It also emerged that many aspects determining the patient's psychological and social sphere are implicated in carotid disease. In conclusion, when taking the multidisciplinary approach that combines human sciences with biological sciences, it is possible to comprehensively define a patient's health and thus improve their clinical management through precision medicine. [ABSTRACT FROM AUTHOR]
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- 2024
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43. Accurate Temperature Reconstruction in Radiofrequency Ablation for Atherosclerotic Plaques Based on Inverse Heat Transfer Analysis.
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Shuang Shu, Guoliang Yang, Hengxin Han, Taijie Zhan, Hangyu Dang, and Yi Xu
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ATHEROSCLEROTIC plaque , *HEAT transfer , *CATHETER ablation , *PYROMETRY , *TEMPERATURE distribution , *PARAMETER estimation - Abstract
Radio frequency ablation has emerged as a widely accepted treatment for atherosclerotic plaques. However, monitoring the temperature field distribution in the blood vessel wall during this procedure presents challenges. This limitation increases the risk of endothelial cell damage and inflammatory responses, potentially leading to lumen restenosis. The aim of this study is to accurately reconstruct the transient temperature distribution by solving a stochastic heat transfer model with uncertain parameters using an inverse heat transfer algorithm and temperature measurement data. The nonlinear least squares optimization method, Levenberg-Marquardt (LM), was employed to solve the inverse heat transfer problem for parameter estimation. Then, to improve the convergence of the algorithm and reduce the computational resources, a method of parameter sensitivity analysis was proposed to select parameters mainly affecting the temperature field. Furthermore, the robustness and accuracy of the algorithm were verified by introducing random noise to the temperature measurements. Despite the high level of temperature measurement noise (ξ = 5%) and larger initial guess deviation, the parameter estimation results remained closely aligned with the actual values, with an overall ERMS consistently below 0.05. The absolute errors between the reconstruction temperature at the measurement points TC1, TC2, and TC3, and the actual temperature, remained within 0.33 °C, 2.4 °C, and 1.17 °C, respectively. The Levenberg-Marquardt algorithm employed in this study proficiently tackled the ill-posed issue of inversion process and obtained a strong consistency between the reconstructed temperature the actual temperature. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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44. Investigating the Added Value of Beck's Depression Inventory in Atherosclerosis Prediction: Lessons from Paracelsus 10,000.
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Dienhart, Christiane, Aigner, Elmar, Iglseder, Bernhard, Frey, Vanessa, Gostner, Isabella, Langthaler, Patrick, Paulweber, Bernhard, Trinka, Eugen, and Wernly, Bernhard
- Subjects
- *
MENTAL illness , *CAROTID artery ultrasonography , *CARDIOVASCULAR diseases , *BECK Depression Inventory , *CEREBROVASCULAR disease , *ATHEROSCLEROTIC plaque - Abstract
Background: Depression is the most common mental illness worldwide and generates an enormous health and economic burden. Furthermore, it is known to be associated with an elevated risk of arteriosclerotic cardiovascular diseases (ASCVD), particularly stroke. However, it is not a factor reflected in many ASCVD risk models, including SCORE2. Thus, we analysed the relationship between depression, ASCVD and SCORE2 in our cohort. Methods: We analysed 9350 subjects from the Paracelsus 10,000 cohort, who underwent both a carotid artery ultrasound and completed a Beck Depression Inventory (BDI) screening. Patients were categorised binomially based on the BDI score. Atherosclerotic carotid plaque or absence was dichotomised for logistic regression modelling. Odds ratios and adjusted relative risks were calculated using Stata. Results: Subjects with an elevated BDI (≥14) had higher odds for carotid plaques compared to subjects with normal BDI, especially after adjusting for classical risk factors included in SCORE2 (1.21; 95%CI 1.03–1.43, p = 0.023). The adjusted relative risk for plaques was also increased (1.09; 95%CI 1.01–1.18, p = 0.021). Subgroup analysis showed an increased odds of plaques with increases in depressive symptoms, particularly in women and patients ≤55 yrs. Conclusions: In our cohort, the BDI score is associated with subclinical atherosclerosis beyond classical risk factors. Thus, depression might be an independent risk factor which may improve risk stratification if considered in ASCVD risk prediction models, such as SCORE2. Furthermore, reminding clinicians to take mental health into consideration to identify individuals at increased atherosclerosis risk may provide added opportunities to address measures which can reduce the risk of ASCVD. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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45. Investigating the potential of catheter‐assisted pulsed focused ultrasound ablation for atherosclerotic plaques.
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Samaddar, Abhirup, Singh, Rohit, Yang, Xinmai, Ebersole, Koji C., and Forrest, M. Laird
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ISCHEMIC stroke , *ADIPOSE tissues , *ATHEROSCLEROTIC plaque , *MYOCARDIAL ischemia , *CORONARY disease - Abstract
Background: Atherosclerosis is a condition in which an adhesive substance called plaque accumulates over time inside the arteries. Plaque buildup results in the constriction of arteries, causing a shortage of blood supply to tissues and organs. Removing atherosclerotic plaques controls the development of acute ischemic stroke and heart diseases. It remains imperative for positive patient outcomes. Purpose: This study sought to develop a minimally invasive technique for removing arterial plaques by applying focused ultrasound (FUS) energy on the metal surface of a nitinol catheter wire to induce inertial cavitation. The induced cavitation can deplete plaque mechanically inside the arteries, leading towards improved recanalization of blood vessels. Methods: The enhanced cavitation effect induced by combining FUS with a metal catheter was first verified by exposing agar phantom gels with or without a 0.9‐mm diameter nitinol wire to an acoustic field produced by a 0.5‐MHz FUS transducer. The phenomenon was further confirmed in pork belly fat samples with or without a 3‐mm diameter nitinol catheter wire. Cavitation was monitored by detecting the peaks of emitted ultrasound signals from the samples using a passive cavitation detector (PCD). Cavitation threshold values were determined by observing the jump in the peak amplitude of signals received by the PCD when the applied FUS peak negative pressure (PNP) increased. To simulate arterial plaque removal, FUS with or without a catheter was used to remove tissues from pork belly fat samples and the lipid cores of human atherosclerotic plaque samples using 2500‐cycle FUS bursts at 10% duty cycle and a burst repetition rate of 20 Hz. Treatment outcomes were quantified by subtracting the weight of samples before treatment from the weight of samples after treatment. All measurements were repeated 5 times (n = 5) unless otherwise indicated, and paired t‐tests were used to compare the means of two groups. A p‐value of <0.05 will be considered significant. Results: Our results showed that with a nitinol wire, the cavitation threshold in agar phantoms was reduced to 2.6 MPa from 4.3 MPa PNP when there was no nitinol wire in the focal region of FUS. For pork belly fat samples, cavitation threshold values were 1.0 and 2.0 MPa PNP, with and without a catheter wire, respectively. Pork belly fat tissues and lipid cores of atherosclerotic plaques were depleted at the interface between a catheter and the samples at 2 and 4 MPa FUS PNP, respectively. The results showed that with a catheter wire in the focal region of a 3‐min FUS treatment session, 24.7 and 25.6 mg of lipid tissues were removed from pork belly fat and human atherosclerotic samples, respectively. In contrast, the FUS‐only group showed no reduction in sample weight. The differences between FUS‐only and FUS‐plus‐catheter groups were statistically significant (p < 0.001 for the treatment on pork belly samples, and p < 0.01 for the treatment on human atherosclerotic samples). Conclusion: This study demonstrated the feasibility of catheter‐assisted FUS therapy for removing atherosclerotic plaques. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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46. Developing Theoretical Models for Atherosclerotic Lesions: A Methodological Approach Using Interdisciplinary Insights.
- Author
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Hofmann, Amun G.
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MARKOV random fields , *STOCHASTIC processes , *ATHEROSCLEROTIC plaque , *CHAOS theory , *MODEL theory - Abstract
Atherosclerosis, a leading cause of cardiovascular disease, necessitates advanced and innovative modeling techniques to better understand and predict plaque dynamics. The present work presents two distinct hypothetical models inspired by different research fields: the logistic map from chaos theory and Markov models from stochastic processes. The logistic map effectively models the nonlinear progression and sudden changes in plaque stability, reflecting the chaotic nature of atherosclerotic events. In contrast, Markov models, including traditional Markov chains, spatial Markov models, and Markov random fields, provide a probabilistic framework to assess plaque stability and transitions. Spatial Markov models, visualized through heatmaps, highlight the spatial distribution of transition probabilities, emphasizing local interactions and dependencies. Markov random fields incorporate complex spatial interactions, inspired by advances in physics and computational biology, but present challenges in parameter estimation and computational complexity. While these hypothetical models offer promising insights, they require rigorous validation with real-world data to confirm their accuracy and applicability. This study underscores the importance of interdisciplinary approaches in developing theoretical models for atherosclerotic plaques. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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47. Endothelial Cell-Derived Cholesterol Crystals Promote Endothelial Inflammation in Early Atherogenesis.
- Author
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Wang, Xia, Fu, Wenxia, Zhou, Guo, Huo, Huanhuan, Shi, Xin, Wang, Hao, Wang, Yinghua, Huang, Xiying, Shen, Linghong, Li, Long, and He, Ben
- Subjects
- *
VASCULAR cell adhesion molecule-1 , *CD54 antigen , *ATHEROSCLEROTIC plaque , *CAROTID artery , *ENDOTHELIAL cells - Abstract
Aims: Endothelial inflammation is crucial in the initiation and progression of atherosclerosis, whereas cholesterol crystals (CCs) play a key role in atherogenesis. However, the effects and origination of CCs on endothelial inflammation are not well understood. Results: In the present study, we found that CCs appeared in the subendothelial space of the partially ligated carotid artery only 1 week after Western diet feeding, which was before immune cell infiltration. In vitro, CCs were generated by human aortic endothelial cells (HAECs) upon low-density lipoprotein treatment. These endothelial cell (EC)-derived CCs increased the expression of intercellular cell adhesion molecule-1 (ICAM1) and vascular cell adhesion molecule-1 (VCAM1). Mechanistic studies demonstrated that CC-induced pyroptosis was critical for endothelial inflammation. Knockdown of gasdermin D (GSDMD) inhibited CC-induced endothelial inflammation, attenuated plaque progression, and decreased macrophage content. Accordingly, the inhibition of GSDMD reduced the CC-induced increase of VCAM1 and ICAM1 in HAECs. Furthermore, CC-mediated pyroptosis was found to be caspase-1 (CASP1)-dependent. Inhibition of CASP1 also reduced endothelial inflammation and attenuated plaque progression. The expression of GSDMD was increased in human atherosclerotic plaques. These findings identify that EC-derived CCs may be an important driving force in the pathogenesis of atherosclerosis. Innovation: This study uncovered a new understanding that CC-induced pyroptosis contributes to endothelial inflammation in early atherogenesis. Conclusion: Targeting endothelial GSDMD or CASP1 contributes to the repression of vascular inflammation and atherogenesis. Antioxid. Redox Signal. 41, 201–215. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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48. Correlation Between Optical Coherence Tomography Angiography Findings at 3 to 6 Weeks and Functional Outcome at 3 Months Following Acute Ischaemic Stroke Due to Extracranial Carotid Artery Atherosclerotic Disease.
- Author
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Mishra, Biswamohan, Pandit, Awadh Kishor, Chawla, Rohan, Aalok, Swati Phuljhele, Shrivastava, M. V. Padma, Nayak, Manoj Kumar, Pm, Yogeesh, Salunkhe, Manish, Garg, Ajay, Srivastava, Achal Kumar, Vishnu, Venugopalan Y., Bhatia, Rohit, Misra, Shubham, Upadhyay, Ashish Datt, and Molla, Kabiruddin
- Subjects
- *
CAROTID artery diseases , *ISCHEMIC stroke , *OPTICAL coherence tomography , *ANGIOGRAPHY , *ATHEROSCLEROTIC plaque ,CAROTID artery stenosis - Abstract
We wanted to evaluate if optical coherence tomography angiography OCTA findings could predict the functional outcome in extracranial carotid artery atherosclerotic disease (ECAD) associated stroke. This exploratory study was performed on adults with acute ischaemic stroke due to ECAD at 3–6 weeks following stroke onset with risk factor matched controls without carotid artery stenosis. Twenty-three stroke patients (cases) and 23 controls were enrolled. There was significant difference between cases and controls in deep vessel density at the macula (p =.0007) and in radial peripapillary capillary perfusion density (RPCPD) at the optic nerve head (ONH) (p =.0007). Statistically significant difference was noted in the total superficial vessel density (SVD) at the macula (SVD within 1 standard deviation [SD] versus SVD beyond 1 SD of control data) in the ipsilateral eye and functional outcome at 3 months (poor versus very good outcome, modified Rankin scale [mRS] 0–1 versus mRS 2–6, respectively; p =.0361). There was statistically insignificant correlation between the RPCPD at the ONH and the National Institutes of Health Stroke Scale score at admission, mRS at discharge, and mRS at 3 months following stroke onset (r =.33, r =.35, r =.39; p =.11, p =.09, p =.06, respectively). The findings of this exploratory study suggested that OCTA findings may predict 3 month outcomes in cases of ECAD-related stroke and could be useful in decision making in future intervention studies as to whether intervene or not in patients having critical or non-critical ECAD for preventing stroke. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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49. Deep learning-based fully automatic screening of carotid artery plaques in computed tomography angiography: a multicenter study.
- Author
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Zhai, D., Liu, R., Liu, Y., Yin, H., Tang, W., Yang, J., Liu, K., Fan, G., Ju, S., and Cai, W.
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CAROTID artery , *COMPUTED tomography , *ATHEROSCLEROTIC plaque , *ANGIOGRAPHY , *RECEIVER operating characteristic curves , *BODY area networks - Abstract
To develop and validate a deep learning (DL) algorithm for the automated detection and classification of carotid artery plaques (CAPs) on computed tomography angiography (CTA) images. This retrospective study enrolled 400 patients (300 in the Center Ⅰ and 100 in Ⅱ). Three radiologists co-labeled CAPs, and their revised calcification status (noncalcified, mixed, and calcified) was regarded as ground truth. Center Ⅰ patients were randomly divided into training and internal validation datasets, while Center Ⅱ patients served as the external validation dataset. Carotid artery regions were segmented using a modified 3D-UNet network, followed by CAPs detection and classification using a ResUNet-based architecture in a two-step DL system. The DL model's detection and classification performance were evaluated on the validation dataset using precision-recall curve, free-response receiver operating characteristic (fROC) curve, Cohen's kappa, and ROC curve analysis. The DL model had achieved 83.4% sensitivity at 3.0 false positives (FPs)/CTA scan in internal validation and 78.9% in external validation. F1-scores were 0.764 and 0.769 at the optimal threshold, and area under fROC curves were 0.756 and 0.738, respectively, indicating good overall accuracy for CAP detection. The DL model also showed good performance for the ternary classification of CAPs, with Cohen's kappa achieved 0.728 and 0.703 in both validation datasets. This study demonstrated the feasibility of using a fully automated DL-based algorithm for the detection and ternary classification of CAPs, which could be helpful for the workloads of radiologists. • The research focuses on automatic classification of CAP. • The DL model can achieve good performance in CAP detection and classification. • The DL model could be applied to deal with reporting workload in the acute setting. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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50. Computed Tomography Angiography Identified High-Risk Coronary Plaques: From Diagnosis to Prognosis and Future Management.
- Author
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Dimitriadis, Kyriakos, Pyrpyris, Nikolaos, Theofilis, Panagiotis, Mantzouranis, Emmanouil, Beneki, Eirini, Kostakis, Panagiotis, Koutsopoulos, George, Aznaouridis, Konstantinos, Aggeli, Konstantina, and Tsioufis, Konstantinos
- Subjects
- *
CORONARY artery disease , *COMPUTED tomography , *PROGNOSIS , *THERAPEUTICS , *ANGIOGRAPHY , *ATHEROSCLEROTIC plaque - Abstract
CT angiography has become, in recent years, a main evaluating modality for patients with coronary artery disease (CAD). Recent advancements in the field have allowed us to identity not only the presence of obstructive disease but also the characteristics of identified lesions. High-risk coronary atherosclerotic plaques are identified in CT angiographies via a number of specific characteristics and may provide prognostic and therapeutic implications, aiming to prevent future ischemic events via optimizing medical treatment or providing coronary interventions. In light of new evidence evaluating the safety and efficacy of intervening in high-risk plaques, even in non-flow-limiting disease, we aim to provide a comprehensive review of the diagnostic algorithms and implications of plaque vulnerability in CT angiography, identify any differences with invasive imaging, analyze prognostic factors and potential future therapeutic options in such patients, as well as discuss new frontiers, including intervening in non-flow-limiting stenoses and the role of CT angiography in patient stratification. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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