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Selenium (Se) deficiency in human and animal nutrition could lead to development of different pathophysiological conditions. Accordingly, biofortification of wheat, as one of the most important food crop, with Se is sustainable strategy for overcome this problem. Although not essential for plants, Se shows dual impact on their metabolism, at low levels it has a beneficial effect, while it is toxic at higher levels. The aim of this study was to elucidate the impact of increased concentrations of selenate and selenite on the oxidative stress and antioxidative response in wheat seedlings (Triticum aestivum L.). As an indicators of oxidative stress, content of H2O2 and lipid peroxidation levels expressed in terms of thiobarbituric acid reactive substances were determined, while antioxidative status was determined by the content of total glutathione and by the activities of antioxidative enzymes such as catalase and ascorbate peroxidase. Results showed that the effect of Se on oxidative stress and antioxidative response in wheat seedlings depended on its chemical form and applied concentration. Both forms of selenium had more significant impact on the measured parameters in the root compared to wheat shoots, suggesting tissue-specific response. Obtained results can serve as a basis for further research on the Se impact on oxidative and antioxidative status in order to get better insight into the wheat detoxification mechanisms.