1. Mild temperature photothermal assisted anti-bacterial and anti-inflammatory nanosystem for synergistic treatment of post-cataract surgery endophthalmitis
- Author
-
Ke Yao, Wanlin Li, Shiyuan Hua, Yuchen Qi, Yang Ye, Hélder A. Santos, Jian He, Min Zhou, Yue Qiao, Danni Zhong, Wei Wang, Andrzej Grzybowski, Hongbo Zhang, Helsinki One Health (HOH), Drug Research Program, Divisions of Faculty of Pharmacy, Division of Pharmaceutical Chemistry and Technology, Nanomedicines and Biomedical Engineering, and Helsinki Institute of Life Science HiLIFE
- Subjects
medicine.medical_treatment ,116 Chemical sciences ,Anti-Inflammatory Agents ,Metal Nanoparticles ,COPPER ,Medicine (miscellaneous) ,02 engineering and technology ,Pharmacology ,0302 clinical medicine ,Endophthalmitis ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,318 Medical biotechnology ,Photothermal effect ,Drug Synergism ,021001 nanoscience & nanotechnology ,Anti-Bacterial Agents ,3. Good health ,Treatment Outcome ,endophthalmitis ,Toxicity ,Rabbits ,221 Nano-technology ,medicine.symptom ,0210 nano-technology ,Research Paper ,Methicillin-Resistant Staphylococcus aureus ,Photothermal Therapy ,medicine.drug_class ,post-cataract Surgery ,INHIBITION ,mild photothermal therapy ,Inflammation ,Cataract Extraction ,Anti-inflammatory ,Benzophenones ,03 medical and health sciences ,Drug Therapy ,INFLAMMATION ,In vivo ,medicine ,Animals ,Humans ,Microbial Viability ,business.industry ,Cataract surgery ,Photothermal therapy ,medicine.disease ,Disease Models, Animal ,antibacterial and anti-inflammation effect ,SILVER ,GOLD NANOPARTICLES ,030221 ophthalmology & optometry ,nanoparticles ,Gold ,3111 Biomedicine ,POSTOPERATIVE ENDOPHTHALMITIS ,business ,Bromobenzenes - Abstract
Rationale: Endophthalmitis, which is one of the severest complications of cataract surgeries, can seriously threaten vision and even lead to irreversible blindness owing to its complicated microenvironment, including both local bacterial infection and severe inflammation. It is urgent to develop a comprehensive treatment for both anti-bacterial and anti-inflammatory effects. Methods: Herein, we developed AuAgCu2O-bromfenac sodium nanoparticles (AuAgCu2O-BS NPs), which was designed to combine anti-bacterial and anti-inflammatory effects for integrated therapy of endophthalmitis after cataract surgery. The AuAgCu2O-BS NPs could eradicate methicillin-resistant Staphylococcus aureus (MRSA) bacterial strain relied on their photodynamic effects and the release of metal ions (Ag+ and Cu+) by the hollow AuAgCu2O nanostructures mediated mild photothermal effects. The anti-inflammatory drug, bromfenac sodium, released from the nanoparticles were able to significantly reduce the local inflammation of the endophthalmitis and promote tissue rehabilitation. In vivo bacterial elimination and anti-inflammation were confirmed by a postcataract endophthalmitis rabbit model. Results: Excellent antibacterial ability of AuAgCu2O-BS NPs was verified both in vitro and in vivo. Ophthalmological clinical observation and pathologic histology analysis showed prominent treatment of inflammatory reaction. Importantly, the mild temperature photothermal effect not only promoted the release of metal ions and bromfenac sodium but also avoided the thermal damage of the surrounding tissues, which was more suitable for the practical application of ophthalmology due to the complex structure of the eyeball. Moreover, superior biocompatibility was approved by the preliminary toxicity investigations, including low cytotoxicity, negligible damage to major organs, and stable intraocular pressure. Conclusions: Our studies of nanosystem provide a promising synergic therapeutic strategy for postcataract endophthalmitis treatment with favorable prognosis and promise in clinical translations.
- Published
- 2020
- Full Text
- View/download PDF