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The present study aimed to optimize the formulation of starch‐based films, exploring the synergy between chitosan (1%, 2% and 3%), glycerol (2.5%, 3% and 3.5%) and concentrated cashew peel extract (1%, 2% and 3%), through a central composite design (23). Evaluating their influence on physical, mechanical and morphological properties. All films produced were flexible and did not present cracks when removed from the plates. Water vapour permeability (WVP) (3.03–7.71 gH2O.mm/m2.h.mmHg) and solubility (1.94%–5.31%) were reduced with the combination of higher levels of extract and chitosan, which also influenced the colour of the films (p < 0.05). Increasing the concentration of cashew peel extract increased tensile strength (TS) and elongation at break (EB) of the films. Morphologically, films with lower levels of cashew peel extract exhibited a uniform and less rough surface. Therefore, our results identified an optimized formulation using the combination of 2% extract, 3% chitosan and 3.5% glycerol. This study expanded the development of starch‐based packaging materials with chitosan, glycerol and the addition of plant extract, which can be used in the preservation of fruits and vegetables. [ABSTRACT FROM AUTHOR]

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Combination therapy integrated with nanotechnology offers a promising alternative for breast cancer treatment. The inclusion of pequi oil, anacardic acid (AA), and docetaxel (DTX) in a nanoemulsion can amplify the antitumor effects of each molecule while reducing adverse effects. Therefore, the study aims to develop pequi oil-based nanoemulsions (PeNE) containing DTX (PDTX) or AA (PAA) and to evaluate their cytotoxicity against triple-negative breast cancer cells (4T1) in vitro. The PeNE without and with AA (PAA) and DTX (PDTX) were prepared by sonication and characterized by ZetaSizer® and electronic transmission microscopy. Viability testing and combination index (CI) were determined by MTT and Chou-Talalay methods, respectively. Flow cytometry was employed to investigate the effects of the formulations on cell structures. PeNE, PDTX, and PAA showed hydrodynamic diameter < 200 nm and a polydispersity index (PdI) of 0.3. The association PDTX + PAA induced a greater decrease in cell viability (~70%, p < 0.0001) and additive effect (CI < 1). In parallel, an association of the DTX + AA molecules led to antagonism (CI > 1). Additionally, PDTX + PAA induced an expressive morphological change, a major change in lysosome membrane permeation and mitochondria membrane permeation, cell cycle blockage in G2/M, and phosphatidylserine exposure. The study highlights the successful use of pequi oil nanoemulsions as delivery systems for DTX and AA, which enhances their antitumor effects against breast cancer cells. This nanotechnological approach shows significant potential for the treatment of triple-negative breast cancer. [ABSTRACT FROM AUTHOR]

Local folks have reported that roasting cashew nuts has fatal effects on domestic birds, especially chickens. This study compared the toxicological effect of acute (one-hour) inhalation of cashew nutshell fumes (CNSF) in Cockerel chickens and Wistar rats. Twenty-four mixed-sex Wistar rats were divided into four groups; control, rats exposed to 500ppm/hr., 1250ppm/hr. and 2500ppm/hr. of CNSF for one (1) hour. The chickens were also divided into three groups of 6 chickens each; control (unexposed chickens), chickens exposed to 500ppm/hr. and 1000ppm/hr. of CNSF for one (1) hour. Hematological analysis as well as histological examinations of the lungs and heart of the Chickens and Rats were carried out. After one hour of exposure, the toxicity signs noted include; scratching, twitching, irregular breathing, and tremors. All rats exposed to 2500ppm/hr of CNSF died with moderate pulmonary congestion. Analysis of hematological parameters in the surviving animals showed a significant (P<0.05) increase in hemoglobin (Hb%), packed cell volume (PCV%) and white blood cells (WBCx109L) counts in both chickens and Wistar rats exposed to CNSF compared with the unexposed control groups. No significant (P>0.05) differences were found between male and female rats for all the hematological parameters measured. The results also showed species-specific variations in all the hematological parameters. Histopathological evaluation reflects a mononuclear inflammatory infiltration of the striated heart muscles and the septa of alveoli in the lungs. These results further highlight the possible adverse health effects of exposure to cashew nut fumes in animals and humans. [ABSTRACT FROM AUTHOR]

Invasive fungal disease causes high morbidity and mortality among immunocompromised patients. Resistance to conventional antifungal drugs and the toxicity associated with high doses highlight the need for effective antifungal therapies. In this study, the antifungal potential of the ethanolic extract of Anacardium occidentale (Cashew Leaf) leaves were evaluated against Candida albicans and C. auris. The antifungal activity was tested by the broth microdilution method and growth kinetic test. To further explore its antifungal action mode, spectrofluorophotometry, confocal microscopy and scanning and transmission electron microscopy were performed. Additionally, heterozygous knockout strains associated with resistance to oxidative stress were included in the study. We found that A. occidentale could inhibit the proliferation and growth of C. albicans at concentrations of 62.5 and 125 μg/mL. The doubling time was also drastically affected, going from 2.8 h to 22.5 h, which was also observed in C. auris. The extract induced the accumulation of intracellular reactive oxygen species (ROS), resulting in endoplasmic reticulum stress and mitochondrial dysfunction, while it did not show cytotoxicity or hemolytic activity at the concentrations evaluated. Our work preliminarily elucidated the potential mechanisms of A. occidentale against C. albicans on a cellular level, and might provide a promising option for the design of a new treatment for invasive candidiasis. [ABSTRACT FROM AUTHOR]

Arboviruses transmitted by mosquito vectors, mainly of the genera Aedes, Anopheles and Culex, are among the main concern for global public health. The rapid spread of these arboviruses causes an increase in government spending, socioeconomic losses and an overload on health systems. Mosquitoes' population control remains the main method to combat the arboviruses spread. This control has been carried out mainly using synthetic pesticides. However, in addition to the possibility of inducing mosquitoes' resistance, synthetic pesticides have harmful effects on both human health and the environment. Thus, the development of eco-friendly products from natural sources, based on agro-industrial by-products, constitutes a viable alternative for the vector mosquitoes' control in immature stages, still in breeding sites. Among the alternatives available, we can highlight the by-products derived from the cashew nut, Anacardium occidentale L. (Anacardiaceae), which has been shown to be a promising alternative due to its ovicidal, larvicidal and insecticidal activities. Considering the efficiency demonstrated both by the by-products of the cashew nut production chain, its constituents, and derived products, the objective of this review is to describe the state-of-the art regarding its use in vector control and to propose future perspectives for the development of products that can be marketed. [ABSTRACT FROM AUTHOR]

Medicinal plants have importance medicinal properties and pharmacological activities. Medicinal value and pharmacological activities of plants are attributed to their bioactive constituents. The efficacy of medicinal plants could not only be considered but also their safety for consumption. The phytochemicals analyses were performed using standard methods. Toxicity tests were conducted using Lorke’s method. The ethanol leaves extract of Azadirachta indica, Anacardium occidentale, and Moringa oleifera contain several phytochemicals. The LD50 value of ethanol leaves extract of Azadirachta indica, Anacardium occidentale, and Moringa oleifera was 3807.89 mg/kg, 4505.55 mg/kg, and above 5000 mg/kg, respectively. The ethanol leaves extract of Anacardium occidentale and Azadirachta indica demonstrated an increase in the levels of ALT, AST, total bilirubin, and globulin, while the Moringa oleifera exhibited a decrease in the levels of ALT, AST, and total bilirubin coupled with increase in total protein and albumin levels. The administration of ethanol leaves extract of Anacardium occidentale and Azadirachta indica increased the levels of urea, creatinine, sodium, potassium, calcium, chlorides, and HCO3- while decreased by the administration of the ethanol leaves extract of Moringa oleifera in rats. The ethanol leaves extract of Azadirachta indica and Anacardium occidentale demonstrated significant decrease in RBC count and PCV while Moringa oleifera exhibited significant increase in RBC count and PCV. Hemoglobin content was significantly decrease by the ethanol leaves extract of Azadirachta indica. The presence of these bioactive metabolites implies that the plants have medicinal properties. The ethanol leaves extract of Moringa oleifera at 5000 mg/kg dose is relatively non-toxic and safe for gavage administration while ethanol leaves extract of Azadirachta indica and Anacardium occidentale are slightly toxic for oral administration.

Cashew trees are often cultivated in semi-arid regions with poor soil and limited rainfall, presenting significant challenges for soil management. This study aimed to evaluate soil fertility based on varying doses of bone meal (0, 250, 500, 750 and 1,000 g pit-1), mixed into the planting pit with or without moisture-retaining hydrogel (0 and 5 g pit-1), in dwarf cashew ‘BRS 226’ cultivation. The soil chemical attributes were monitored over two growing seasons. The bone meal application increased the soil pH by 221 % along the two years and boosted the organic matter by 13 % in the first year and 28 % in the second one. The use of 5 g of hydrogel per pit, combined with 1,000 g of bone meal per pit, is recommended to improve the soil fertility in semi-arid cashew-growing areas over a two-year period. The bone meal fertilization in cashew planting promotes beneficial changes in the soil, and the hydrogel has a great potential in cashew farming, offering an alternative for expanding the fruit production in regions with low rainfall and sandy soils.

Cashew (Anacardium occidentale) is one of the main tropical fruit trees, and its chestnuts are the most consumed in the world. Cashew orchards are managed under irrigated and unirrigated conditions, and pesticides are applied manually (hand sprayer) or by a tractor‐powered sprayer. The chestnut moth Anacampsis phytomiella (Lepidoptera: Gelechiidae) is the primary pest that attacks cashew chestnuts, causing losses of up to 53% in yield in the orchards. Sampling plans and economic injury levels (EILs) are part of the decision‐making systems of integrated pest management (IPM) programmes. The EIL varies according to the method of insecticide spraying (hand sprayer or tractor) and in irrigated and unirrigated cashew orchards.This study aimed to determine the EIL and a sequential sampling plan for A. phytomiella in cashew orchards.Decision‐making systems were determined using data collected from 44 orchards over 3 years. EILs ranged from 0.53 to 1.53 for chestnuts attacked by the pest in bunches with 10 chestnuts, depending on irrigation and insecticide spray methods. The sequential sampling plan was validated using field‐collected data (providing correct decisions in 100% of the fields and an average time saving of 83.65%), operational characteristic (OC) and average sample number curves.In conclusion, determined decision‐making systems can be incorporated into IPM programmes for A. phytomiella in cashew orchards, as they enable correct, rapid and cost‐effective pest control decisions. [ABSTRACT FROM AUTHOR]

Maintenance of the quality and hygiene of maxillofacial prosthesis allows to maintain the health of the residual tissues. Sampling of the maxillofacial prostheses has relieved presence of microbial colonization on silicone surfaces. Cleaning procedures of maxillofacial silicones are done using mechanical means or using adjunctive with chemical means. Cleaning with a 2–4% chlorhexidine gluconate spray or dipping in solution for a minute and then washing under running water can sufficiently condition to reduce the amount of bacterial contamination. Due to rising microorganism resistance and fewer adverse effects, phytoextracts appear to be a viable option. Additionally, the use of excipients derived from plants is provides new opportunities for the pharmaceutical industry into the creation of innovative pharmaceutical products that are sustainable. To evaluate and compare the leaf extracts of Mangifera indica (M.indica), Anacardium occidentale (A.occidentale) and 0.2% chlorhexidine gluconate (CHX) on disinfection of maxillofacial silicone material surface contaminated with Staphylococcus aureus (S.aureus) and Candida albicans (C.albicans). Of the 150 maxillofacial silicone elastomer silicone samples, 75 samples were contaminated with S. aureus and 75 with C.albicans. The contaminated disc was rolled on blood agar and pre-disinfection Colony Forming Units (CFU) were evaluated followed by subjecting the discs to disinfection protocols. The contaminated discs with S. aureus and C.albicans were disinfected using M.indica leaf extracts, A.occidentale leaf extracts and 0.2% CHX for 10 min. Post-disinfection CFUs were evaluated by rolling the disc on blood agar. The results were tabulated and analysed using dependent t -test, one-way ANOVA and Tukeys multiple posthoc procedure. Pair-wise comparison of pre-and post-disinfection log CFU counts of S.aureus gave a statistical significance between 0.2% CHX and and M.indica leaf extract. No statistically significant results were found between 0.2% CHX and A.occidentale. Pair wise comparison of the log CFU from pre-disinfection to post-disinfection of C.albicans gave a statistical significance between all the three groups. In the present study A.occidentale leaf extract and M.indica leaf extract have shown significant reduction in CFU of both the organisms. 0.2% CHX showed the most CFU reduction post disinfection of maxillofacial silicone material surface contaminated S.aureus and C.albicans followed by A.occidentale leaf extracts and M.indica leaf extracts. Given the limitations of the current research, A.occidentale leaf extract and M.indica leaf extract can be used as an alternative for disinfection of maxillofacial silicone prosthesis. [Display omitted] [ABSTRACT FROM AUTHOR]

Epidemics of cashew powdery mildew are observed every year in most of the growing regions in Northeast Brazil. Currently, fungicide management is the main disease control strategy. This study aimed to compare powdery mildew epidemics according to the flowering periods in 2014 and 2019, in different dwarf cashew clones in 2018 and 2021, and with or without chemical control in 2016 and 2020. The disease severity data were used to calculate the variables area under the disease progress curve, final disease and onset of the epidemics, and the monomolecular linear model was used to calculate the initial disease incidence and disease progress rate. Regarding the flowering effect, it was found that, in 2014, the first flowering period stood out from the others for initial disease incidence, disease progress rateand area under the disease progress curve. In 2019, the third flowering period showed the lowest levels of initial disease incidence, area under the disease progress curve and final disease. As for the clones, ‘BRS 226’ stood out as the most resistant for most the disease variables in the two years of the study. The fungicide sprays had a significant effect and prevented the powdery mildew epidemics in the two years of the study. The powdery mildew epidemics showed to be more severe in the first flowering period and can be reduced by using chemical control and more resistant dwarf cashew clones.

Context: Uncontrolled practice of antibiotics leads to bacterial resistance. Therefore, investigating the possible utilization of several medicinal plant extracts is necessary. Traditionally, numerous medicinal plants have been widely utilized to treat some human diseases including Anacardium occidentale. Aims: To investigate the antibacterial activity of the ethanolic extract and fractions from A. occidentale as well as characterize the phytochemical profile of A. occidentale leaves associated with antibacterial capacity. Methods: The antibacterial activity was determined using minimum inhibitory concentration and time kill assay, which confirmed by streak agar plate. Phytochemical profiling of A. occidentale leaves extract and fractions were evaluated by their total phenolic contents, total flavonoid contents, terpenoid contents, and metabolite analysis by UHPLC-HR-ESI-MS. Results: Among the extract and all fractions, the ethyl acetate fraction had high killing efficiency against Staphylococcus aureus, Bacillus subtillis, Escherichia coli, and Pseudomonas aeruginosa, which was confirmed by its MIC value of 0.5, 0.5, 0.5, and 0.25 mg/mL, respectively. Corresponding to its antibacterial capacity, the ethyl acetate fraction showed the highest of phenolic (508.89 mg GAE/g dw) and flavonoid (184 mg QE/g dw) contents. Based on HRMS investigation, the extract and fractions contain amino acids, phenolic acids, alkaloids, flavonoids, fatty acid amides, fatty acids, and organic acids as classes of molecules. Moreover, avicularin/quercetin-3-O-arabinofuranoside, myricitrin, and gallic acid were identified as major compounds from the active fraction. Conclusions: The ethyl acetate fraction showed the most remarkable antibacterial activity. There was a significant correlation between the antibacterial activity of active fraction and its phenolic and flavonoid contents.

Abstract Cashew nut is a globally popular nut not only for its delicious and crunchy flavor but also for bioactive compounds, which present positive benefits to human health. Different parts of cashew have characteristic compounds. The kernel of cashew nut is rich in fatty acids, the testa is rich in polyphenols, cashew apple is rich in polyphenols and carotenoids, and phenols with aliphatic long chain are present in the cashew nutshell liquid. Therefore, the edible part of cashew possesses a wide range of bioactive compounds, with reported biological properties, including anti‐tumor, brain health, cardiovascular and cerebrovascular protection, anti‐diabetic, gastroprotection, pathophysiological disorders protection, and antioxidant. On the other hand, the inedible part of cashew, the cashew nutshell liquid, could play an important role in the industry as surface coatings, insulating formulations and so on. Hence, there are considerable demands to increase the added value of cashew, thereby exploring greater economic value. Thus, this review summarizes nutrients, bioactive compounds, biological functions, and applications of Anacardium occidentale to provide a theoretical basis and inspiration for further in‐depth studies and utilization.

Combination therapy integrated with nanotechnology offers a promising alternative for breast cancer treatment. The inclusion of pequi oil, anacardic acid (AA), and docetaxel (DTX) in a nanoemulsion can amplify the antitumor effects of each molecule while reducing adverse effects. Therefore, the study aims to develop pequi oil-based nanoemulsions (PeNE) containing DTX (PDTX) or AA (PAA) and to evaluate their cytotoxicity against triple-negative breast cancer cells (4T1) in vitro. The PeNE without and with AA (PAA) and DTX (PDTX) were prepared by sonication and characterized by ZetaSizer® and electronic transmission microscopy. Viability testing and combination index (CI) were determined by MTT and Chou-Talalay methods, respectively. Flow cytometry was employed to investigate the effects of the formulations on cell structures. PeNE, PDTX, and PAA showed hydrodynamic diameter < 200 nm and a polydispersity index (PdI) of 0.3. The association PDTX + PAA induced a greater decrease in cell viability (~70%, p < 0.0001) and additive effect (CI < 1). In parallel, an association of the DTX + AA molecules led to antagonism (CI > 1). Additionally, PDTX + PAA induced an expressive morphological change, a major change in lysosome membrane permeation and mitochondria membrane permeation, cell cycle blockage in G2/M, and phosphatidylserine exposure. The study highlights the successful use of pequi oil nanoemulsions as delivery systems for DTX and AA, which enhances their antitumor effects against breast cancer cells. This nanotechnological approach shows significant potential for the treatment of triple-negative breast cancer.

• 80 % methanolic extract of A. occidentale roots (80 % MAO) was subjected to column purification using solvents n-hexane, ethyl acetate, and methanol with varying ratios. • Structural characterization performed using advanced spectroscopic techniques includes: UV–visible spectroscopy, FT-IR, 1H NMR, 13C NMR, and HRMS. • Gallic acid identity was revealed in 80 % MAO. • HPLC profiling accounted for the presence of polyphenols: picein, epicatechin, caffeic acid, taxifolin, ferulic acid, and naringenin. • The identified phytocompounds was impactful antidiabetic agents against diabetes-associated molecular targets - 6F8F, 6PZ9, 3EEK, 4PYP, 1B2Y, 2QMJ, 3S41, 4MRA 5PZX, 3IOL, and 5YCP. Anacardium occidentale, a member of the Anacardiaceae family, is a tropical plant rich in secondary metabolites, predominantly, polyphenols. The plant is renowned for its diverse pharmacological properties. The present study was undertaken to identify potent antidiabetic phytocompounds present in A. occidentale roots and to evaluate their antidiabetic effects through molecular docking studies involving selected therapeutic antidiabetic targets. The chemical profiling of 80 % methanolic extract of A. occidentale roots (80 % MAO) was carried out by column chromatography using n-hexane, ethyl acetate, and methanol (with varying ratios). This was followed by structural characterization using advanced spectroscopic techniques and HPLC profiling leading to the isolation and identification of gallic acid. Additionally, polyphenols including picein, epicatechin, caffeic acid, taxifolin, ferulic acid, and naringenin were also identified from the extract. Through molecular docking analysis, these compounds were engaged with diabetes-associated molecular targets (6F8F, 6PZ9, 3EEK, 4PYP, 1B2Y, 2QMJ, 3S41, 4MRA 5PZX, 3IOL, 5YCP), underscoring their potential as impactful antidiabetic agents. Hence, it is inferred that it is the synergistic action of these compounds that contributes to the antidiabetic effect of 80 % MAO. This study advocates the utilization of these phytocompounds as potent antidiabetic medications, either as individual entities or as combinatorial therapy with synthetic drugs or phytocompounds themselves, thereby suggesting promising avenues for future research and therapeutic applications. [ABSTRACT FROM AUTHOR]

The current study aimed to identify the chemical constituents and bioactivities of the crude ethanolic extract (CEE) and its fractions (ethyl acetate (EAF), hexane (HEF), and aqueous (AEF)) from leaves of cashew (Anacardium occidentale L.) grown in Vietnam. A total of 31 compounds which belong to alkanes, hydrocarbons, iodine, terpenoids, phenolics, and flavonoids were determined by a gas chromatography–mass spectrometry (GC–MS) analysis, with bis(2‐ethylhexyl) phthalate being the most prevailing compound. The highest total phenolic and flavonoid contents were obtained in the EAF, followed by HEF, CEE, and AQF. All samples showed promising in vitro antibacterial activity, enzyme inhibition, and anticancer activity. Among the samples tested, the EAF exhibited the highest enzyme inhibition activity against α‐amylase and α‐glucosidase (IC50 values of 51.24 μg/mL and 99.29 μg/mL, respectively), cytotoxicity activity against HeLa cells (IC50 value of 79.49 μg/mL), and antibacterial activity against Bacillus subtilis and Escherichia coli with MIC values of 5 mg/mL and 2.5 mg/mL, respectively. These findings suggest that the leaves of A. occidentale cultivated in Vietnam are a promising source of bioactive components and that EAF is a promising bioactive material warranting further pharmaceutical investigation. [ABSTRACT FROM AUTHOR]

Plant parasitic nematodes (PPNs) cause great damage to all agricultural crops; they infect all plant parts and cause reduction in yield. Synthetic nematicides have been used lately to combat the menace of PPNs on farmlands. The drawbacks of synthetic nematicides gave rise to interest in alternative methods of managing plant parasitic nematodes. In this regard, compounds from biodegraded as well as fresh stem bark of (cashew) Anacardium occidentale were extracted, isolated and partially characterized. The crude extracts and isolates were tested for their nematicidal activity in laboratory and field experiments. Significant (p<0.05) differences were observed in the activity of the extracts and isolates. The isolated compounds from A. occidentale biodegraded stem bark extract (ANCO/BDG/CMP) exhibited higher juvenile mortality and reduced egg hatch in the laboratory while the activity of the isolated compound from fresh bark (ANCO/FRS/CMP) extract was significantly lower. The crude extracts ANCO/FRS/CRD and ANCO/BDG/CRD had weaker nematicidal activity. Meloidogyne incognita population was notably low after harvest in all treated cabbage plants on the field. Spectroscopic results revealed that microbial action during biodegradation of the stem bark produced a different compound having nematicidal properties. [ABSTRACT FROM AUTHOR]

This study assessed the genetic diversity of 120 selected cashew plants from Nachingwea and Newala traditional cashew populations, comprising of 60 cashew plants from each population. Eight Simple Sequence Repeats (SSR) makers were used to ascertain the extent of genetic variation within and between the two traditional cashew populations. Genomic DNA extraction and Polymerase Chain Reaction (PCR) were performed using standard protocols. The results showed that all SSR markers were polymorphic. Average Polymorphic Information Content (PIC), heterozygosity, number of alleles per locus, allelic frequency and Shannon's Index were 0.540, 0.721, 3.391, 0.666 and 0.649, respectively. Analysis of molecular variance results showed that genetic variation within populations was 88% while genetic variation between populations was 12%, implying gene flow between the populations. The results further showed that the two cashew populations are genetically diverse and that Nachingwea traditional cashew population was genetically more diverse than Newala population. High genetic variation observed in the cashew populations shows that they are suitable for use in cashew breeding programmes. Thus, conservation of traditional cashew populations is recommended. [ABSTRACT FROM AUTHOR]

Invasive fungal disease causes high morbidity and mortality among immunocompromised patients. Resistance to conventional antifungal drugs and the toxicity associated with high doses highlight the need for effective antifungal therapies. In this study, the antifungal potential of the ethanolic extract of Anacardium occidentale (Cashew Leaf) leaves were evaluated against Candida albicans and C. auris. The antifungal activity was tested by the broth microdilution method and growth kinetic test. To further explore its antifungal action mode, spectrofluorophotometry, confocal microscopy and scanning and transmission electron microscopy were performed. Additionally, heterozygous knockout strains associated with resistance to oxidative stress were included in the study. We found that A. occidentale could inhibit the proliferation and growth of C. albicans at concentrations of 62.5 and 125 μg/mL. The doubling time was also drastically affected, going from 2.8 h to 22.5 h, which was also observed in C. auris. The extract induced the accumulation of intracellular reactive oxygen species (ROS), resulting in endoplasmic reticulum stress and mitochondrial dysfunction, while it did not show cytotoxicity or hemolytic activity at the concentrations evaluated. Our work preliminarily elucidated the potential mechanisms of A. occidentale against C. albicans on a cellular level, and might provide a promising option for the design of a new treatment for invasive candidiasis.

Background and aims: Cashew is an evergreen tropical tree native to South America. It has spread to several locations around the globe, including Africa. The plant’s various parts have been effective in treating a variety of diseases. This study aims to assess the nephroprotective effect of aqueous Anacardium occidentale cashew apple juice on the kidney of paracetamol (PCM)-induced injury in albino rats. Methods: Wistar rats were used to conduct this study. Group 1 was given saline as usual. While groups 3, 4, and 5 were administered 1, 2, and 4 ml/kg of the aqueous extract, group 2 received normal saline. In groups 2-5, animals received oral PCM 2000 mg/kg body weight on the eighth day. All animals were weighed again and sacrificed under a light diethyl ether vapor 20 hours after the last dose administration. Results: The extract significantly (P < 0.05) raised packed cell volume (PCV), white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb), and platelet values compared to group 2. There was no significant difference (P > 0.05) in the weight-to-body size ratio of rats in none of the groups given extracts (P < 0.05) when compared to group 2. Creatinine and urea level were considerably lower in the groups who received extract. Additionally, histological research supports biochemical parameters. Conclusion: The study shows that A. occidentale cashew apple may help manage renal disorders and protect the kidney against hazardous substances.

In semi-arid regions, where seasonal drought or irregular rainfall distribution are common occurrences, agricultural management techniques or strategies, such as the use of bone meal and/or hydrogel, can mitigate adverse conditions and ensure crop production. This study aimed to evaluate the effects of bone meal doses (0, 250, 500, 750 and 1,000 g pit-1), in association or not with hydrogel (0 and 5 g pit-1, respectively), on the leaf nutrient content of the dwarf cashew cultivar BRS 226. The leaf nutrient contents were evaluated during two growing seasons. For being a good source of macronutrients, the bone meal increased the levels of N, P, Ca, K and Mg in the dwarf cashew leaves. The leaf contents of the micronutrients Zn, Fe and Mn decreased with increasing bone meal doses. The nutrient accumulation in the leaves of the dwarf cashew planted in pits with bone meal and hydrogel has the following order: N > K > Ca > Mg > P > Mn > Fe > Zn > Cu. The application of bone meal at levels close to 600 g pit-1, mainly with the use of hydrogel, is a viable fertilizer alternative for dwarf cashew.

Depression presents a significant global health burden, necessitating the search for effective and safe treatments. This investigation aims to assess the antidepressant effect of the hydroethanolic extract of Anacardium occidentale (AO) on depression-related behaviors in rats. The depression model involved 42 days of unpredictable chronic mild stress (UCMS) exposure and was assessed using the sucrose preference and the forced swimming (FST) test. Additionally, memory-related aspects were examined using the tests Y-maze and Morris water maze (MWM), following 21 days of treatment with varying doses of the AO extract (150, 300, and 450 mg/kg) and Imipramine (20 mg/kg), commencing on day 21. The monoamines (norepinephrine, serotonin, and dopamine), oxidative stress markers (MDA and SOD), and cytokines levels (IL-1β, IL-6, and TNF-α) within the brain were evaluated. Additionally, the concentration of blood corticosterone was measured. Treatment with AO significantly alleviated UCMS-induced and depressive-like behaviors in rats. This was evidenced by the ability of the extract to prevent further decreases in body mass, increase sucrose consumption, reduce immobility time in the test Forced Swimming, improve cognitive performance in both tests Y-maze and the Morris water maze by increasing the target quadrant dwelling time and spontaneous alternation percentage, and promote faster feeding behavior in the novelty-suppressed feeding test. It also decreased pro-inflammatory cytokines, corticosterone, and MDA levels, and increased monoamine levels and SOD activity. HPLC-MS analysis revealed the presence of triterpenoid compounds (ursolic acid, oleanolic acid, and lupane) and polyphenols (catechin quercetin and kaempferol). These results evidenced the antidepressant effects of the AO, which might involve corticosterone and monoaminergic regulation as antioxidant and anti-inflammatory activities. [ABSTRACT FROM AUTHOR]

The study describes the synthesis of zinc oxide (ZnO) nanoparticles using Anacardium occidentale cashew apple juice. The synthesized ZnO nanoparticles were characterized by UV–vis spectroscopy, FTIR spectroscopy, X-ray diffraction spectroscopy, and scanning electron microscopy. The unsophisticated observation revealed bio-reduction of Zn2+ by dark reddish Zn0 formation that settled down. The dark reddish precipitate was turned into white precipitates of ZnO nanoparticles while treated with NaOH. UV–visible spectroscopy represented the formation of ZnO nanoparticles, with an absorption band optimized at 284 nm. FTIR analysis shows the functional groups of biomolecules (protein, carbohydrate, and polyphenol) and their bio-reduction and capping processes. XRD analysis confirms the nanoparticles are highly crystalline, with poly-crystalline zinc oxides and hexagonal wurtzite types. SEM analyses revealed the flower shape of the ZnO nanoparticle's size ranges from 106.7 to 169.2 nm. The anticandidal and antibiofilm activity of ZnO nanoparticles against fluconazole-resistant clinical isolates of C. glabrata were evaluated. The ZnO nanoparticle revealed strong anticandidal activity against C. glabrata with an MIC of 48 µg/mL. The sub-MIC doses (4, 8, and 16 µg/mL) of ZnO nanoparticles showed significant antibiofilm activity against fluconazole-resistant clinical isolates of C. glabrata, with maximum biofilm reduction obtained at 16 µg/mL. Thus, the present study concludes that ZnO nanoparticles are a potential agent for the prevention of C. glabrata biofilm, especially the biofilm formed surface of a prosthetic device. [ABSTRACT FROM AUTHOR]

Introduction: Anacardium occidentale or cashew are popular traditional food and have raised research interest for complementary cancer treatment. Cancer has become leading cause of death and treatment involved severe side effects. In present study, we aim to study the anti-proliferative effects of cashew shoots in breast cancer (MDAMB-231), colorectal cancer (HT-29) and liver cancer (HepG2) cell lines. Methods: Cell lines were treated with 70% ethanolic cashew extract for cytotoxicity test with MTT assay. AO/PI dual fluorescent assay and RNase/PI staining were used to determine apoptosis induction effects. Phytochemicals screening was carried out by using gas chromatography mass spectrometry (GCMS) and liquid chromatography mass spectrometry (LCMS). Results: The cytotoxicity assay of cashew shoot extract demonstrated IC50 of 81.1 ± 0.11 µg/ml for MDA-MB-231, 307.5 ± 2.31 µg/ml for HT-29 and 272.6 ± 1.91 µg/ml for HepG2 cell lines. The apoptotic bodies include chromatin condensation, cell blebbing and nuclear fragmentation and apoptosis induction were shown by AO/PI staining. There was significant increase of cell count in sub-G0 phase in MDA-MB-231 cell lines treated with cashew shoot extract. It was demonstrated that cashew shoot extract contained 38 compounds from GCMS such as sitosterol, tannin, pyrogallol, phenol and 20 compounds from LCMS such as citric acid, gallic acid, myricetin and hinokiflavone that may give rise to its anti-cancer effect. Conclusion: Cashew shoot extract demonstrated potential anti-cancer properties thus further study is required to investigate its mechanism as anti-cancer agent. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate infestation of Anacampsis phytomiella Busck on different genotypes of the dwarf cashew, its impact on nut production, and the effect of rainfall on the production and infestation of A. phytomiella. The study was carried out during the 2014 to 2019 agricultural years, in an experimental plantation of dwarf cashew, in Pacajus, Ceará, Brazil. The IC 13/1, IC 13/2, IC 13/3 and IC 13/4 progeny were evaluated, with two commercial clones, CCP 76 and BRS 226, used as controls. The percentage of punctured nuts as well as the productivity of each genotype were estimated. The progeny/clones differed both in relation to the percentage of damaged nuts and in relation to productivity. For both variables, the IC 13/1 and IC 13/3 progeny had the highest values, while the CCP 76 clone presented the lowest mean values. There was a significant positive correlation between rainfall and nut infestation and production. A positive correlation was also seen between production and the percentage of infestation. The results would therefore suggest that the IC 13/1 and IC 13/3 progenies present characteristics related to a tolerance to attack by, and/or development of A. phytomiella. [ABSTRACT FROM AUTHOR]

Purpose: To evaluate the effects of pretreatment with different crosslinking agents on glass-fiber-post adhesive luting. Materials and Methods: Single-rooted human teeth (n = 20) were randomly assigned to four groups: proanthocyanidins (PA) from grape-seed extract, cardol and cardanol (separated from cashew nut-shell liquid) and negative control (hydroethanolic solution). The solutions were applied on 37% phosphoric acid-etched dentin for 60 s. Glassfiber posts were cemented using a three-step etch-and-rinse adhesive (Scotchbond Multi-Purpose, 3M Oral Care) and composite cement (RelyX ARC, 3M Oral Care). Slices for the push-out bond strength test were cut and tested after 24-h or 6-month storage in distilled water. The dentin underlying the adhesive layer was analyzed by micro-Raman spectroscopy to evaluate vibrational formation of collagen crosslinks. Three additional slices per group were also made and the adhesive in-situ degree of conversion (DC) was analyzed by micro-Raman spectroscopy. The results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Results: No statistically significant changes in bond strength were found over time for any of the groups, except with cardol, which increased bond strength (8.4 ± 3.9 MPa at 24 h to 15.0 ± 2.9 MPa after 6 months, p < 0.001) after aging. The formation of peaks at 1117 cm-1 and 1235 cm-1 showed the presence of collagen crosslinks for all three biomodification agents. The DC outcomes showed no statistically significant differences between groups (p = 0.514). Conclusion: Biomodification agents did not impair adhesive polymerization. Cardol demonstrated a positive influence on intraradicular dentin bonding for glass-fiber post luting. [ABSTRACT FROM AUTHOR]

The rising of diseases caused by multidrug‐resistant bacteria has encouraged researchers to explore more antimicrobial substances, as well as chemicals capable of potentiating the action of existing ones against multidrug‐resistant bacteria. Anacardium occidentale produces a fruit known as cashew nut, filled with a dark, almost black, caustic, and flammable liquid called cashew nutshell liquid (CNSL). The goal of the study was to evaluate the intrinsic antimicrobial activity of the major compounds present in CNSL, called anacardic acids (AA), as well as their possible modulatory action as an adjuvant of Norfloxacin against a Staphylococcus aureus strain overproducing the NorA efflux pump (SA1199B). Microdilution assays were performed to determine the minimum inhibitory concentration (MIC) of AA against different microbial species. Norfloxacin and Ethidium Bromide (EtBr) resistance modulation assays were performed in the presence or absence of AA against SA1199‐B. AA showed antimicrobial activity against Gram‐positive bacterial strains tested but no activity against Gram‐negative bacteria or yeast strains. At subinhibitory concentration, AA reduced the MIC values for Norfloxacin and EtBr against the SA1199‐B strain. Furthermore, AA increased the intracellular accumulation of EtBr in this NorA overproducer strain, indicating that AA are NorA inhibitors. Docking analysis showed that AA probably modulates Norfloxacin efflux by spatial impediment at the same binding site of NorA. [ABSTRACT FROM AUTHOR]

The cashew peduncle has a high nutritional value and contains a wide variety of phenolic compounds. Among these, anacardic acids (AnAc) are biologically active components; however, they influence the cashew juice flavor and, consequently, its acceptance. This study validates a high-performance liquid chromatography method for quantifying the AnAc present in cashew peduncles, using a C18 reverse-phase column and a diode-array detector. The calibration curve obtained showed satisfactory precision for intraday (CV = 0.20%) and interday (CV = 0.29%) quantification, linearity (y = 2333.5x + 2956.2; r2 = 0.9979), repeatability with respect to retention time (CV = 0.45%) and area (CV = 0.30%), and selectivity, and possessed detection and quantification limits of 0.18 and 0.85 µg·mL−1, respectively. Different cashew clones containing AnAc were extracted and analyzed using the proposed method. A recovery of >90% was achieved using two sequential extractions. The total AnAc content ranged from 128.35 to 217.00 mg·100 g−1 in peduncle samples obtained from five different cashew clones. [ABSTRACT FROM AUTHOR]

In order to enhance the value of edible fruit trees from the Sudanian zone, such as cashew, a study was carried out in the Logone Oriental province in Chad. Ethnobotanical and socioeconomic surveys, through structured and semi-structured interviews, were carried out with 18 resource persons, who were the owners and/or managers. It appears that the cashew nut sector is relatively young in this region. Nevertheless, the price per kg of nuts has evolved positively over the last three years (2019 to 2021), ranging from 186±44.11 FCFA (2019) to 386±75.93 FCFA (2021). Maximum production is recorded in plantations over 15 years old in Pendé and Nya respectively with 600 kg/year and 617 kg/year (2019), 700 kg/year and 625 kg/year (2020), 550 kg/year and 668 kg/year (2021). Seven traces of anthropization were encountered and it appears that young plantations (less than 10 years old, 10 to 15 years old) are more anthropized, but also, the species Anacardium occidentale L. is highly anthropized in the study area than other species. The study revealed that, despite a favourable eco-geographical setting for cashew cultivation, local populations are not interested in the crop and do not have enough land for it. A development plan for these agrosystems should be envisaged. [ABSTRACT FROM AUTHOR]

Cardol, as one of the major constituents of Cashew Nut Shell Liquids has received little attention in terms of modification and applications. The presence of two hydroxyl groups and an alkyl side chain of 15 carbon length make it a natural compound of interest. The present study is aimed at modification and complexation of cardol, and the antimicrobial investigations of the compound as well as its analogue and complexes. Isolated cardol from cashew nut shell liquid was nitrated, and the compounds were used in the synthesis of metal (II) complexes of Copper, Cobalt, and Zinc. The compounds and complexes were characterised by melting points, UV-Vis spectrophotometry, Fourier Transform Infrared (FTIR) spectrophotometry, and Nuclear Magnetic resonance (NMR) spectroscopy. The antibacterial and antifungal evaluations of both the compounds and complexes were tested in vitro against some selected bacteria and fungi. From the results, Copper complex of nitrocardol produced significantly higher antibacterial zone of inhibition (11.5 ± 2.1 mm) against Pedobacter mendelii than tetracycline while Copper complex of cardol showed significantly higher antifungal activity (13.5 ± 2.1 mm) against Rhizopus stolonifar than clotrimazole (10.0 ± 0.0 mm). It was generally observed that modification and metal complexation enhanced the antimicrobial activity of cardol. Further studies on these complexes could be done to enhance their potencies and potentials for use as antibacterial and antifungal agents. [ABSTRACT FROM AUTHOR]

Cashew (Anacardium occidentale L.) leaf is traditionally used to treat skin infections. Although many flavonols have been identified from its leaf extract, their inhibitory effects on skin pathogens are not yet determined. The aims of this study were to determine the antimicrobial (against skin pathogenic microbes) and antioxidant activities of four flavonol glycosides from the crude extract and three flavonol aglycones from the hydrolyzed extract. The hydrolyzed extract was found to show higher activities than the crude extract. Myricetin showed the highest activity against all the tested bacteria and yeast with the lowest Minimum Inhibition Concentration (MIC) of 7.81 μg/mL on Corynebacterium minutissimum ATCC23348. Myricetin also exhibited good primary antioxidant activities with the effective concentration with 50% of activity (EC50) values ranged between 2.23 μg/mL and 6.40 μg/mL. The highest secondary antioxidant activity was indicated by myricetin-3-O-rhamnoside. Thus, myricetin can be considered as a bioactive compound of the hydrolyzed extract. [ABSTRACT FROM AUTHOR]

Newcastle disease (ND) is one of the most important diseases of poultry worldwide. The aims of this study was to investigate the effects of methanol leaf extract of Anacardium occidentale (MLEAO) on the hematology of chickens infected with velogenic Newcastle disease virus (vNDV). A total of 200 6-week-old cockerels, randomly assigned to eight groups (A, B, C, D, E. F, G, and H) of 25 each, were used for the study. Groups A, B, and C chickens (prophylactic groups) were drenched orally with MLEAO at the doses of 300, 150, and 75 mg/kg body weight, respectively for 7 days before challenge with vNDV. Chickens in all groups, except group H, were then challenged with vNDV. When clinical signs of Newcastle disease appeared, groups D, E, and F chickens (therapeutic groups) were drenched with 300, 150, and 75 mg/kg MLEAO, respectively for 7 days. Group G was untreated but challenged while group H was untreated and unchallenged. Hematological evaluations followed standard procedures. The study showed that prophylactic treatment at 300, 150, and 75 mg/kg and therapeutic treatment at 300 mg/kg led to significant (p < 0.05) higher white blood cell count but prophylactic treatment at 300 mg/kg, led to significantly (p < 0.05) lowering of the erythrocytic profile. It was concluded that prophylactic administration of Anacardium occidentale leaf extract at all doses as used in this study and its therapeutic administration at 300 mg/kg enhanced white blood cell mobilization and response to challenge with vNDV infection in chickens. [ABSTRACT FROM AUTHOR]

Preparing electrode materials plays an essential role in the fabrication of high-performance supercapacitors. In general, heteroatom doping in carbon-based electrode materials enhances the electrochemical properties. Herein, nitrogen, oxygen, and sulfur co-doped porous carbon (PC) materials were prepared by direct pyrolysis of Anacardium occidentale (AO) nut-skin waste for high-performance supercapacitor applications. The as-prepared AO-PC material possessed interconnected micropore/mesopore structures and exhibited a high specific surface area of 615 m2 g−1. The Raman spectrum revealed a moderate degree of graphitization of AO-PC materials. These superior properties of the as-prepared AO-PC material help to deliver high specific capacitance. After fabricating the working electrode, the electrochemical performances including cyclic voltammetry, galvanostatic charge–discharge, and electrochemical impedance spectroscopy measurements were conducted in 1 M H2SO4 aqueous solution using a three-electrode configuration for supercapacitor applications. The AO-PC material delivered a high specific capacitance of 193 F g−1 at a current density of 0.5 A g−1. The AO-PC material demonstrated <97% capacitance retention even after 10,000 cycles of charge–discharge at the current density of 5 A g−1. All the above outcomes confirmed that the as-prepared AO-PC from AO nut-skin waste via simple pyrolysis is an ideal electrode material for fabricating high-performance supercapacitors. Moreover, this work provides a cost-effective and environmentally friendly strategy for adding value to biomass waste by a simple pyrolysis route. [ABSTRACT FROM AUTHOR]

Anacardium occidentale commonly known as Cashew is a plant that is widely used in African traditional medicine. It is endowed with phytochemical constituents that are responsible for its medicinal properties. Twenty-five male Wistar rats were grouped as follows: Control (Group A), Group B (L-NAME 40 mg/kg), Group C (100 mg/kg Anacardium occidentale extract plus 40 mg/kg L-NAME), Group D (200 mg/kg extract plus 40 mg/kg L-NAME) and Group E (10 mg/kg of Lisinopril plus 40 mg/kg L-NAME). The animals were treated with oral administration of either the extracts or Lisnopril daily for 4 weeks. Neuro-behavioural tests such as the Morris Water Maze and Hanging Wire Grip tests were carried out to evaluate memory/spatial learning and muscular strength, respectively. Makers of oxidative stress, antioxidant enzymes and immunohistochemical staining of Glial Fibrillary Acidic Protein and Ionised Calcium Binding Adaptor molecule 1 were assessed. L-NAME administration caused significant increases in biomarkers of oxidative stress, decreased antioxidant status, acetylcholinesterase activity, altered neuro-behavioural changes, astrocytosis, and microgliosis. However, Anacardium occidentale reversed exaggerated oxidative stress biomarkers and improved neuro-behavioural changes. Combining all, Anacardium occidentale enhanced brain antioxidant defence status, improved memory and muscular strength, thus, suggesting the neuroprotective properties of Anacardium occidentale. [ABSTRACT FROM AUTHOR]

Anthracnose caused by Colletotrichum gloeosporioides affects the leaves, inflorescences, nuts, and peduncles of cashew trees (Anacardium occidentale). The use of genetically improved plants and the insertion of dwarf cashew clones that are more resistant to phytopathogens are strategies to minimize the impact of anthracnose on cashew production. However, resistance mechanisms related to the biosynthesis of secondary metabolites remain unknown. Thus, this study promoted the investigation of the profile of volatile organic compounds of resistant cashew clone leaves ('CCP 76', 'BRS 226' and 'BRS 189') and susceptible ('BRS 265') to C. gloeosporioides, in the periods of non-infection and infection of the pathogen in the field (July-December 2019 - Brazil). Seventy-eight compounds were provisionally identified. Chemometric analyses, such as Principal Component Analysis (PCA), Discriminating Partial Least Squares Analysis (PLS-DA), Discriminating Analysis of Orthogonal Partial Least Squares (OPLS-DA), and Hierarchical Cluster Analysis (HCA), separated the samples into different groups, highlighting hexanal, (E)-hex-2-enal, (Z)-hex-2-en-1-ol, (E)-hex-3-en-1-ol, in addition to α-pinene, α-terpinene, γ-terpinene, β-pinene, and δ-3-carene, in the samples of the resistant clones in comparison to the susceptible clone. According to the literature, these metabolites have antimicrobial activity and are therefore chemical marker candidates for resistance to C. gloeosporioides in cashew trees. [ABSTRACT FROM AUTHOR]

Successful weight management represents a challenge to obesity control. Evidence suggests that nut consumption promotes a prolonged satiety response. Therefore, we hypothesize that nuts could be associated with greater weight loss in comparison to a control group, and we evaluate the acute and long-term effects of Brazilian nuts (BN: 15 g of Brazil nuts + 30 g of cashew nuts) included in an energy-restricted intervention on food intake, appetite, and peptide hormones. We conducted an 8-week, randomized, open-label, controlled, parallel-arm clinical trial with 28 women at cardiometabolic risk who received an energy-restricted diet containing BN or an energy-restricted nut-free diet (control). At baseline and after 8 weeks of intervention, subjective postprandial appetite ratings were assessed using a visual analog scale (VAS) before and after consumption (0, 10, 60, 120, 180, and 240 minutes) of a 437-kcal nut-enriched (BN group) or nut-free (control) breakfast meal. Subsequently, an ad libitum lunch was served, and the participants completed another VAS at 280 minutes. Plasma concentrations of ghrelin, glucose-dependent insulinotropic polypeptide, glucagon-like peptide 1, and leptin were measured at fasting and postprandially at 60, 120, and 240 minutes. Last, subsequent reported 24-hour energy intake was assessed in a free-living setting. BN consumption did not have acute effects on food intake, appetite, or peptide hormones. However, after an 8-week intervention, postprandial ghrelin (difference between post- and preintervention area under the curve) decreased in the BN group in comparison to the control (mean difference, 1978 pg/mL/min, 95% CI, 27-3929 pg/mL/min; P =.047) and therefore, an energy-restricted diet containing demonstrated potential to decrease hunger in cardiometabolic risk women. The Brazilian Nuts Study is an 8-week, randomized, open-label, controlled, parallel-arm clinical trial in women at cardiometabolic risk. Our findings showed that the consumption of Brazilian nuts ([BN] 15 g of Brazil nuts + 30 g of cashew nuts) did not alter food intake or appetite. On the other hand, an energy-restricted diet containing BN decreased ghrelin (difference between post- and preintervention area under the curve [ΔAUC]) after 8 weeks. ↓ reduction; ↔ no change; GIP, glucose-dependent insulinotropic polypeptide; GLP-1, glucagon-like peptide 1. [Display omitted] [ABSTRACT FROM AUTHOR]

Introduction: Morphological parameters of flowering are fundamental in the reproductive process of plants, but this subject is poorly explored in the cashew tree Anacardium occidentale. Objective: To determine the influence of the flowering and harvest period on floral parameters, and to identify association with hermaphrodite flowers in the dwarf cashew. Methods: For the 2018 and 2019 harvests in 120 samples we measured number of male/hermaphrodite/abnormal flowers; panicle biomass, length, maximum width, and ramifications at 30, 45 and 60 days for 360 samples in total. Results: The harvest effect was not significant. Panicle length and width (at 30 days), had the greatest contributions to the production of hermaphrodite flowers. The presence of male flowers (at 45 days), and the panicle length and number of primary branches (at 60 days) were the main factors at their respective periods. Conclusions: The emission of hermaphrodite flowers responds negatively to male flowers. Variations in flowering compromise the production of hermaphrodite flowers and the flowering structure. [ABSTRACT FROM AUTHOR]

Introduction: the flowers, leaves, peels, fruits, and nuts of A. occidentale are rich in phenolic compounds and flavonoids, their high anti-inflammatory and antioxidant potential offers protection to cells. The pseudo fruit and bark of A. occidentale have polyphenols, mainly tannins, which act as natural antibiotics. Objective: to analyze the antioxidant, antimicrobial, anti-inflammatory and antifungal potential of Anacardium occidentale L. Methodology: this is an integrative review of the literature that searches the databases: ScienceDirect (Elsevier), Google Scholar (Google) and Redalyc (Scholarly Scientific Information System Redalyc), from July to August 2022. The articles underwent a selection process for the elimination of duplicates using Mendeley Desktop and then by the free software Rayyan QCRI, read in full and the main data analyzed and collected after the Critical Assessment of evidence hierarchy of evidence for intervention studies. Ten items were selected, following the temporal design. Results: four articles talked about antioxidant activity, seven about antimicrobial activity, one on anti-inflammatory and antifungal activity, some articles report more than one therapeutic potential. Conclusion: studies have shown that Anacardium occidentale has medicinal potential, mainly in the use of leaves, bark and gums, and can act as an inhibitor of inflammatory sites, bacterial species such as Escherichia coli and Staphylococcus aureus. In addition to promoting antioxidant action due to the presence of flavonoids, tannins, polyphenols, and vitamin C. [ABSTRACT FROM AUTHOR]

The cashew (Anacardium occidentale L.) is a tree with multiple and varied interests, which contribute to the socio-economic development of Côte d'Ivoire. Although the first plantations were intended to combat environmental degradation in the savannah regions, cashew has now become a real cash crop in the country. The objective of this study was to characterise cashew-based cropping systems (social and agronomic characteristics) in Côte d'Ivoire, with a view to fostering their improvement. To this end, a survey was conducted among 1182 cashew producers, based on four production zones (Centre, North, North-East and North-West). The results showed that cashew producers are predominantly farmers with an average age of between 45 and 50 years, with a predominance of men. Most producers rotate crop combinations at the juvenile stage of the trees (< 10 years). In addition, crop rotations vary across agro-ecological zones. Overall, cereals are the main crop in rotations in the north (67.5%) and northwest (74.8%), with maize predominating. In contrast, tubers, with yams predominate the rotations in the Centre (59.8%) and North-East (78.8%) zones of the country. [ABSTRACT FROM AUTHOR]

The skin is the largest organ that performs a variety of the body's essential functions. Impairment of skin structure and functions during the aging process might severely impact our health and well-being. Extensive evidence suggests that reactive oxygen species play a fundamental role in skin aging through the activation of the related degradative enzymes. Here, the 16 Thai medicinal plant species were screened for their potential anti-skin aging properties. All extracts were investigated for total phenolic and flavonoid contents, antioxidant, anti-elastase, and anti-tyrosinase activities, as well as the binding ability of compounds with target enzymes by molecular docking. Among all the plants screened, the leaves of A. occidentale and G. zeylanicum exhibited strong antioxidants and inhibition against elastase and tyrosinase. Other potential plants include S. alata leaf and A. catechu fruit, with relatively high anti-elastase and anti-tyrosinase activities, respectively. These results are also consistent with docking studies of compounds derived from these plants. The inhibitory actions were found to be more highly positively correlated with phenolics than flavonoids. Taken together, our findings reveal some Thai plants, along with candidate compounds as natural sources of antioxidants and potent inhibitors of elastase and tyrosinase, could be developed as promising and effective agents for skin aging therapy. [ABSTRACT FROM AUTHOR]

The red and white types of Anacardium occidentale L. stem barks (AoB) are used in the Community of Portuguese Language Countries, including Portugal, to make traditional herbal preparations for the treatment of diabetes. This work aims to obtain the macroscopic, micromorphological, chemical, and purity data necessary to use both types of AoBs as medicinal plants safely. Macroscopically, a brown inner (red AoB) or a dark-brown inner surface (white AoB) was observed. Light and scanning electron microscopy showed that sclereid cells with thin cell walls were significantly larger (p < 0.001) in red AoB than in white AoB, but calcium oxalate druses and starch grain areas were significantly larger (p < 0.001) in white AoB than in red AoB. The chromatographic profiles (thin-layer chromatography and high-performance liquid chromatography) of both types of AoBs were characterized by the presence of gallic and protocatechuic acids and other phenolic derivatives. The condensed tannins were the major secondary metabolites class (143.69 ± 4.67 mg and 73.79 ± 4.46 mg catechin equivalents/g AoB in red and white types, respectively). The loss on drying and the total ash were, respectively, 7.07 ± 0.16% and 2.31 ± 0.18% in red AoB and 6.58 ± 0.16% and 1.94 ± 0.14% in white AoB. The obtained data are helpful in ensuring the quality of AoB as raw material for the pharmaceutical market. [ABSTRACT FROM AUTHOR]

Recently, natural antioxidants for the food industry have become an important focus. Cashew nut-shell liquid (CNSL) is composed of compounds that can act as natural antioxidants in food systems. The aim of this work was to evaluate the potential of CNSL and its components to act as natural antioxidants in a bulk oil system. CNSL was treated with calcium hydroxide to obtain two fractions [cardol/cardanols acid fraction (CCF) and anacardic acid fraction (AF)]. CNSL, FF and AF were analyzed by thin-layer chromatography and Fourier-transform infrared spectroscopy. The protective effects of CNSL, CCF and AF were tested in terms of the peroxide value of bulk soybean oil in accelerated assays and were compared against controls with and without synthetic antioxidants (CSA and CWA). CNLS, CCF, AF and CSA were tested at 200 mg/kg soybean oil by incubation at 30, 40, 50 and 60 °C for five days. The activation energy (Ea) for the production of peroxides was calculated by using the linearized Arrhenius equation. Thin-layer chromatography and Fourier-transform infrared spectroscopy revealed that (i) CNSL contained cardanols, anacardic acids, and cardols; (ii) CCF contained cardanols and cardols; and (iii) AF contained anacardic acids. CSA (Ea 35,355 J/mol) was the most effective antioxidant, followed by CCF (Ea 31,498 J/mol) and by CNSL (Ea 26,351 J/mol). AF exhibited pro-oxidant activity (Ea 8339 J/mol) compared with that of CWA (Ea 15,684 J/mol). Therefore, cardols and cardanols from CNSL can be used as a natural antioxidant in soybean oil. [ABSTRACT FROM AUTHOR]

In Côte d'Ivoire, the stem borer, Apate terebrans, is one of the three main insect pests of cashew (Anacardium occidentale), an important cash crop. But, the lack of scientific knowledge about its ecology is a real obstacle to the development of efficient control methods. This study aimed to know the seasonal fluctuation of A. terebrans, in relation to the phenological stage of cashew and some climatic factors. The cryptic life of the pest led to the evaluation of its abundance by counting monthly the openings of fresh galleries that it digs in the cashew tree. Systematic sampling was carried out on 1924 cashew trees distributed in 10 orchards in the departments of Bondoukou and Bouna in the North-East of Côte d'Ivoire, from July 2018 to June 2020. Climatic data were recorded over this period. The first individuals of A. terebrans were observed in July in the rainy season; peak numbers were reached in November-December in the dry season, before decreasing considerably in January and becoming nil in March-April. Individuals were significantly more abundant at cashew flowering. Significant differences were observed between the abundances within and between departments and from year to year. Simple regression analyses showed that temperature favours the growth of pest populations, while rainfall and hygrometry are unfavourable. This study contributes to the effective control of A. terebrans, and provides insights into the determinants of the variability of A. terebrans infestations from one orchard to another. [ABSTRACT FROM AUTHOR]

Angular leaf spot of cashew tree in Brazil has been attributed to pigmented and nonpigmented strains of Xanthomonas citri pv. anacardii. Due to the possibility of dissemination of the disease by propagating material, it is necessary to understand the survival mechanisms of the causal agent. Thus, the present study aimed to characterize the behavior of viable but non-cultivable cells (VBNC) in two pigmented strains (CCRMTAQ13 and CCRMTAQ18) and one nonpigmented strain (IBSBF2579) of X. citri pv. anacardii, integrating in silico, in vitro, and in vivo studies. Thirteen genes associated with the VBNC phase were identified in the genomes of these strains. The log phase was observed at 24, 48, and 120 h for CCRMTAQ13, CCRMTAQ18, and IBSBF2579 strains, respectively. The death phase was observed at 96 h for both pigmented strains and 168 h for the nonpigmented strain. Using qPCR analyses, it was possible to characterize the occurrence of VBNC for the three strains. When inoculated, the strains showed 100% incidence during the VBNC phase, with the IBSBF2579 strain having the longest incubation period (IP). The strains did not differ concerning final severity (FS) in the VBNC phase. To our knowledge, this is the first report of the occurrence of the VBNC mechanism in X. citri pv. anacardii strains. Furthermore, it has been demonstrated that X. citri pv. anacardii in the VBNC state is potentially infective when they meet their host's apoplast, which points to the need to use integrated practices to detect this bacterium in cashew seedlings. [ABSTRACT FROM AUTHOR]

The anatomical features of the bud and graft unions of three accessions of Anacardium occidentale (Brazilian Jumbo, Brazilian extra-large, and Indian madras) were studied to determine the compatibility and incompatibility of the unions. Scion, stock, and union diameters of graft and bud were measured and the data obtained were used to discriminate between the compatible and incompatible combinations. The histological features of successful unions revealed a necrotic layer 4 days after propagation. Days 20 and 60 showed the formation of the callus cells, the cambium bridge was also observed 100 days after the propagation. The unsuccessful unions showed a wide gap in the cortex of the unions. Wedge graft and T-bud unions showed 72.92% and 33.33% successes respectively. The results showed that budding and grafting compatibility exists among the three accessions of A. occidentale. The factors that were responsible for the success of the unions were elucidated. [ABSTRACT FROM AUTHOR]

Cashew (Anacardium occidentale L.) is a cross-pollinating plant whose fruit consists of two parts, the nut, and the apple. This study aimed to carry out agromorphological characteristics of cashew fruits to establish relationships with their physicochemical and nutritional properties. Thirty (30) cashew accessions fruits were sampled in different regions of Burkina Faso. The length, width, thickness, weight, yield, kernel output ratio (KOR), proximate composition, specific minerals, phenolic compounds, and anti-nutritional factors were assessed. Evaluations were made using standard methods. The results showed significant variations in agro-morphological, physicochemical and nutritional traits depending on the accessions and the organ. Also, the ratio of apple mass versus nut one was about 12.24 ± 1.24. Kernels are an important source of proteins, fat, total phenolic compounds, and flavonoids, with average contents of 22.84 ± 1.25 g/100 g, 51.65 ± 2.54 g/100 g, 9.78 ± 2.15 mg GAE/g, and 6.24 ± 12.15 mg QE/g, respectively. As for the apples, they contained substantial quantities of Potassium, Phosphorus, Chlorine, and Magnesium with contents of 611.24 ± 14.5 mg/100 g, 418.24 ± 16.47 mg/100 g, 332.24 ± 10.54 mg/100 g, and 224.95 ± 13.15 mg/100 g, respectively. Statistical analyses showed that mineral and phytate contents were positively correlated to cashew apples while phenolic compounds and tannins were strongly correlated with walnuts. Principal component analyses showed three groups of accessions based on apples and kernels characteristics. These data showed a direct relationship between cashew physicochemical, nutritional potentials, kernels, and apples' agro-morphological characteristics. These data constitute an important basis for the identification of cashew accessions with high nutritional and economic potential. [ABSTRACT FROM AUTHOR]

Depression presents a significant global health burden, necessitating the search for effective and safe treatments. This investigation aims to assess the antidepressant effect of the hydroethanolic extract of Anacardium occidentale (AO) on depression-related behaviors in rats. The depression model involved 42 days of unpredictable chronic mild stress (UCMS) exposure and was assessed using the sucrose preference and the forced swimming (FST) test. Additionally, memory-related aspects were examined using the tests Y-maze and Morris water maze (MWM), following 21 days of treatment with varying doses of the AO extract (150, 300, and 450 mg/kg) and Imipramine (20 mg/kg), commencing on day 21. The monoamines (norepinephrine, serotonin, and dopamine), oxidative stress markers (MDA and SOD), and cytokines levels (IL-1β, IL-6, and TNF-α) within the brain were evaluated. Additionally, the concentration of blood corticosterone was measured. Treatment with AO significantly alleviated UCMS-induced and depressive-like behaviors in rats. This was evidenced by the ability of the extract to prevent further decreases in body mass, increase sucrose consumption, reduce immobility time in the test Forced Swimming, improve cognitive performance in both tests Y-maze and the Morris water maze by increasing the target quadrant dwelling time and spontaneous alternation percentage, and promote faster feeding behavior in the novelty-suppressed feeding test. It also decreased pro-inflammatory cytokines, corticosterone, and MDA levels, and increased monoamine levels and SOD activity. HPLC-MS analysis revealed the presence of triterpenoid compounds (ursolic acid, oleanolic acid, and lupane) and polyphenols (catechin quercetin and kaempferol). These results evidenced the antidepressant effects of the AO, which might involve corticosterone and monoaminergic regulation as antioxidant and anti-inflammatory activities.

Diarrhea is a prevalent gastrointestinal problem associated with fatal implications. It is a huge public health concern that requires better alternatives to current drugs. This study investigated the mechanisms involved in the antidiarrheal activity of Anacardium occidentale (Ao) stem bark extract, a plant commonly used in the management of diarrhea in Nigeria. Methanolic stem bark extract of the plant was partitioned into three fractions: hexane fraction, ethyl acetate fraction (AoEF) and methanol fraction. In vitro studies on the effect of these fractions on guinea pig ileum (GPI) strips, as well as the modulatory effect of AoEF on standard agonists- and antagonists-induced GPI contraction and relaxation, revealed AoEF as the most active fraction. In vivo studies to assess the effect of AoEF on the dopaminergic, muscarinic, and serotonergic pathways were carried out using gastric emptying (GE) and gastrointestinal transit (GT) as experimental end points. AoEF was subjected to GC-MS analysis, while the identified compounds were docked with the muscarinic acetylcholine receptor M3 (CHRM3) using AutodockVina. Results indicated that AoEF inhibited GE and GT via inhibition of CHRM3. In addition, GC-MS analysis revealed the presence of 24 compounds in AoEF, while docking indicated that octadecanoic acid 2-(2-hydroxylethoxy) ethyl ester exhibited the highest binding affinity to CHRM3. This study indicated that the antidiarrheal activity of Ao is through its antimotility effect via the inhibition of the muscarinic pathway. And since none of the identified compounds exhibited higher binding affinity to CHRM3 relative to loperamide, the antimotility activity of these phytoconstituents may be via synergism.

Cashew (Anacardium occidentale L.) is a cross-pollinating plant whose fruit consists of two parts, the nut, and the apple. This study aimed to carry out agro-morphological characteristics of cashew fruits to establish relationships with their physicochemical and nutritional properties. Thirty (30) cashew accessions fruits were sampled in different regions of Burkina Faso. The length, width, thickness, weight, yield, kernel output ratio (KOR), proximate composition, specific minerals, phenolic compounds, and anti-nutritional factors were assessed. Evaluations were made using standard methods. The results showed significant variations in agro-morphological, physicochemical and nutritional traits depending on the accessions and the organ. Also, the ratio of apple mass versus nut one was about 12.24 ± 1.24. Kernels are an important source of proteins, fat, total phenolic compounds, and flavonoids, with average contents of 22.84 ± 1.25 g/100 g, 51.65 ± 2.54 g/100 g, 9.78 ± 2.15 mg GAE/g, and 6.24 ± 12.15 mg QE/g, respectively. As for the apples, they contained substantial quantities of Potassium, Phosphorus, Chlorine, and Magnesium with contents of 611.24 ± 14.5 mg/100 g, 418.24 ± 16.47 mg/100 g, 332.24 ± 10.54 mg/100 g, and 224.95 ± 13.15 mg/100 g, respectively. Statistical analyses showed that mineral and phytate contents were positively correlated to cashew apples while phenolic compounds and tannins were strongly correlated with walnuts. Principal component analyses showed three groups of accessions based on apples and kernels characteristics. These data showed a direct relationship between cashew physicochemical, nutritional potentials, kernels, and apples’ agro-morphological characteristics. These data constitute an important basis for the identification of cashew accessions with high nutritional and economic potential.