Search

Adolescence is a critical developmental period when the brain is plastic, and stress exposure can have lasting physiological consequences. One mechanism through which adolescent stress may have lasting effects is by altering microRNAs (miRNAs), leading to wide-scale gene expression changes. Three prior independent studies used unbiased approaches (RNA sequencing or microarray) to identify miRNAs differentially expressed by chronic variable stress in male rodents. In all three studies, miRNA-200a was differentially expressed in areas of the brain associated with emotion regulation. The current study extends this research to determine if chronic non-variable adolescent stress downregulates miRNA-200a expression by looking at two strains (BALB/cJ and C57BL/6J) of male and female mice. We utilized a 14-day (2 h/day) restraint stress protocol and verified stress effects on adolescent body weight gain and circulating corticosterone concentrations relative to non-restraint controls. Mice were then left undisturbed until they were euthanized in adulthood, at which time brains were collected to measure miRNA-200a in the ventral hippocampus. Three weeks after adolescent stress ended, differences in body weight between groups were no longer significant; however, animals exposed to stress had less miRNA-200a expression in the ventral hippocampus than control animals. These data implicate miRNA-200a expression as a potential mechanism by which adolescent stress can have persistent impacts on multiple outcomes in both male and female mice. [ABSTRACT FROM AUTHOR]

Pregnancy and the postpartum period induce physiological changes that can influence women's cognitive functions. Alzheimer's disease (AD) has a higher prevalence in women and is exacerbated by early life stress. In the present study, we found that late adolescent social isolation combined with the experience of pregnancy and delivery accelerates the onset of cognitive deficits in 5xFAD dams, particularly affecting their ability to recognize novelty. These cognitive deficits manifested as early as 16 weeks, earlier than the usual timeline for these mice, and were closely associated with increased levels of corticosterone, suggesting dysregulation of the hypothalamic--pituitary--adrenal (HPA) axis. Notably, the presence of β-amyloid plaques in brain regions associated with novelty recognition did not significantly contribute to these deficits. This highlights the potential role of stress and HPA axis dysregulation in the development of cognitive impairments related to AD, and underscores the need for further investigation. [ABSTRACT FROM AUTHOR]

The present study aimed to explore the psychological correlates of premenstrual syndrome in menarcheal females of rural and urban schools of Shimla, Himachal Pradesh (120 rural & 120 urban). The present paper takes into account two psychological correlates which are adolescent stress and trait anxiety and efforts are made to find out the predictive role of these psychological traits in premenstrual syndrome in females. In the rural sample, adolescent stress has significantly and positively contributed 35% of the variance in dysphoria, 23% of the variance in somatic distress and 27% of the variance in cognitive problems (sub-dimensions of PMS) and trait-anxiety has significantly and positively contributed 7% of the variance in dysphoria, 8% of the variance in somatic distress and 7% of the variance in cognitive problem. In the urban sample, adolescent stress has significantly and positively contributed total 10% of the variance in dysphoria, 12% of the variance in somatic distress and 18% of the variance in cognitive problems and trait-anxiety has significantly and positively contributed total 4% of the variance in dysphoria, 12% of the variance in somatic distress and 9% of the variance in cognitive problem. The prevalence of PMS was found more in the rural sample than the urban sample. [ABSTRACT FROM AUTHOR]

Pregnancy and the postpartum period induce physiological changes that can influence women’s cognitive functions. Alzheimer’s disease (AD) has a higher prevalence in women and is exacerbated by early life stress. In the present study, we found that late adolescent social isolation combined with the experience of pregnancy and delivery accelerates the onset of cognitive deficits in 5xFAD dams, particularly affecting their ability to recognize novelty. These cognitive deficits manifested as early as 16 weeks, earlier than the usual timeline for these mice, and were closely associated with increased levels of corticosterone, suggesting dysregulation of the hypothalamic–pituitary–adrenal (HPA) axis. Notably, the presence of β-amyloid plaques in brain regions associated with novelty recognition did not significantly contribute to these deficits. This highlights the potential role of stress and HPA axis dysregulation in the development of cognitive impairments related to AD, and underscores the need for further investigation.

Adolescence is a time of intense cortical development and a period of heightened sensitivity to insult. To determine how sex affects the short- and long-term outcomes of early-adolescent stress exposure, we subjected prepubescent (postnatal day 30) male and female mice to repeated multiple concurrent stressors (RMS). In the posterior parietal cortex (PPC), RMS caused the elimination of excitatory synapses in deeper layers while inhibitory synapse density was predominantly diminished in superficial layers. These short-term effects coincided with reduced visuo-spatial working memory and were similar in both sexes. The loss of excitatory synapses and impaired working memory persisted in males past a 30-day recovery period. In contrast, we observed a remarkable recovery of excitatory transmission and behavioral performance in females. Inhibitory synapse density recovered in both sexes. We have also observed a late onset anxiety phenotype in RMS exposed females that was absent in males. Overall, our results indicate that there are marked sex differences in the long-term effects of prepubescent stress on cortical synapses and behavior.

Adolescent stress is complex and impairing. Novel measures are needed to understand stress variability within individuals over time from a physiological as well as a subjective perspective. To test the feasibility of combining ecological momentary assessment (EMA) and wearable biosensors to assess adolescent stress, using an idiographic approach with experience sampling methods. A small sample (n = 23) of predominantly African American, economically disadvantaged adolescents were asked to complete EMA surveys four times per day and wear an armband measuring Heart rate (HR) and skin response for a 2‐week period. Descriptive analyses examined number of hours of armband wear, percentage of viable data, and percentage of surveys completed by gender and race. Associations between biosensor data and EMA reports within and across individuals were also examined using hierarchical linear regression. EMA survey completion was good (81%) as was adherence to biosensor protocols, although technological difficulties interfered with collection of HR variability for youth with darker skin tones. Youth reported stressful events in 12.79% of EMA surveys, although 43% reported no negative mood experiences. Convergent validity was supported for detecting between‐person (EMA) and within‐person (EMA and biosensors) variability in stress across time, although associations across youth were highly variable. Results suggest value for biobehavioral methods in understanding day‐to‐day stress in adolescents but highlight variability in stress experiences as well as technological limitations, especially for youth of colour. [ABSTRACT FROM AUTHOR]

We tested a conceptual model examining associations between prenatal substance exposure and adolescent cortisol reactivity profiles in response to an acute social evaluative stressor. We included cortisol reactivity in infancy, and direct and interactive effects of early‐life adversity and parenting behaviors (sensitivity, harshness) from infancy to early school age on adolescent cortisol reactivity profiles in model testing. Participants were 216 families (51% female children; 116 cocaine‐exposed) recruited at birth, oversampled for prenatal substance exposure, and assessed from infancy to early adolescence (EA). Majority of participants self‐identified as Black (72% mothers, 57.2% adolescents), and caregivers were primarily from low‐income families (76%), were single (86%), and had high school or below education (70%) at recruitment. Latent profile analyses identified three cortisol reactivity patterns including elevated (20.4%), moderate (63.1%), and blunted (16.5%) reactivity groups. Prenatal tobacco exposure was associated with higher likelihood of membership in the elevated reactivity compared to the moderate reactivity group. Higher caregiver sensitivity in early life was associated with lower likelihood of membership in the elevated reactivity group. Prenatal cocaine exposure was associated with higher maternal harshness. Interaction effects among early‐life adversity and parenting indicated that caregiver sensitivity buffered, and harshness exacerbated, the likelihood that high early adversity would be associated with the elevated and blunted reactivity groups. Results highlight the potential importance of prenatal alcohol and tobacco exposure for cortisol reactivity and the role of parenting as exacerbating or buffering the impact of early‐life adversity on adolescent stress response. [ABSTRACT FROM AUTHOR]

The influence of personality factors, school achievement goals, academic efficacy, and stressload of serious problems on final grades was studied by giving a questionnaire, The Perceived Stress and Control Assessment Scale, to 915 Swedish high school students. A new measure, Stressload Index, was created by extending the Control-Stress Index. Structural Equation Modeling analysis revealed that the variance in final grades was explained by four predictors, namely academic efficacy, stressload of serious problems, neuroticism, and extraversion, to 13%, academic efficacy contributing most. The effect of stressload of serious problems was shown to be negative. The influence of extraversion was negative, whereas neuroticism had a positive effect on final grades. [ABSTRACT FROM AUTHOR]

Objectives: The impact of chronic exposure to environmental adversities on brain regions involved in cognition and mental health depends on whether it occurs during the perinatal period, childhood, adolescence or adulthood. The effects of these adversities on the brain and behavior arise as a function of the timing of the exposure and their co-occurrence with the development of specific regions. Here we aimed to explore the behavioral phenotypes derived from two nutritional stress paradigms which differed in the timing of exposure: a low-protein perinatal diet during gestation and lactation and a low-protein diet during adolescence. Methods: Locomotor and exploratory activity, recognition memory and aversive memory were measured in CF-1 8-week-old male mice subjected to perinatal malnutrition (LP-P) or adolescent malnutrition (LP-A), and their respective controls with normal protein diet (NP-P and NP-A). Results: By using the open field test, we found that LP-P and LP-A mice showed reduced exploratory activity compared to controls, but no alterations in their locomotor activity. Recognition memory was impaired only in LP-P mice. Interestingly, aversive memory was not altered in LP-P mice but was enhanced in LP-A mice. Considering the stress-inoculation theory, we hypothesized that protein malnutrition during adolescence represents a challenging but still moderate stressful environment, which promotes active coping in face of later adversity. Conclusion: Our results indicate that while perinatal malnutrition impairs recognition memory, adolescent malnutrition enhances aversive memory, showing dissimilar adaptive responses. [ABSTRACT FROM AUTHOR]

Nasroallah Moradi-Kor,1,2 Masoomeh Dadkhah,3 Ali Ghanbari,2 Hadi Rashidipour,4 Ahmad Reza Bandegi,5 Mehdi Barati,6 Parviz Kokhaei,7 Ali Rashidy-Pour2,8 1Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran; 2Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran; 3Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran; 4School of Veterinary Medicine, Islamic Azad University, Garmsar, Iran; 5Laboratory of Endocrine Research, Research Center of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; 6Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; 7Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran; 8Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, IranCorrespondence: Ali Rashidy-Pour Laboratory of Learning and MemoryResearch Center of Physiology, Semnan, University of Medical Sciences, Semnan 15131-38111, IranTel +98-9121140221Email Rashidy-Pour@semums.ac.irBackground and Purpose: Ample evidence indicates that chronic adolescence stress is associated with an increased risk of developing neuropsychiatric disorders in adulthood. Given the importance of the effective therapeutic ways to overcome adolescent stress-related deficits, the present study investigated the effects of Spirulina platensis (SP), environmental enrichment (EE), and voluntary exercise (EX) and their combination on anxiety or depression-like behaviors, oxidative stress, and alterations of BDNF and 5HT-3 receptors in the prefrontal cortex (PFC) induced by adolescent stress in adult female rats.Methods: During the adolescent period (PNDs30-40), rats were subjected to restraint stress. Then, the animals were subjected to SP treatment (200 mg/kg/day), EX, EE, and the combined treatments (SP+EX, and SP+EE) for 15 days between PNDs41-55. Subsequently, anxiety or depression-like behaviors, BDNF levels, oxidative stress markers and mRNA expression of BDNF and 5HT3 in the PFC were assessed.Results: Stressed rats demonstrated enhanced anxiety levels and depression-like behaviors in adulthood. Regarding the oxidative stress markers, stressed rats exhibited significantly higher levels of malondialdehyde, a lipid peroxidation product, higher activities of antioxidant enzymes (glutathione peroxidase and superoxide dismutase) and significantly lower total antioxidant reactivity capacity in the PFC. Additionally, adolescent stress significantly increased 5HT3 receptor mRNA expression and decreased BDNF content and its mRNA expression in the PFC. Treatments with SP, EX, EE, and the combined interventions alleviated these deficits.Conclusion: Our findings indicate that appropriate interventions during the adolescent period can protect against adolescent stress-induced behavioral, and biochemical defects and oxidative stress damage in adulthood.Keywords: adolescent stress, anxiety, depression, environmental enrichment, physical activity spirulina, oxidative stress

Nasroallah Moradi-Kor,1,2 Ali Ghanbari,2 Hadi Rashidipour,3 Ahmad Reza Bandegi,4 Behpour Yousefi,5 Mehdi Barati,6 Parviz Kokhaei,7 Ali Rashidy-Pour2 1Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran; 2Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran; 3School of Veterinary Medicine, Islamic Azad University, Garmsar, Iran; 4Laboratory of Endocrine Research, Research Center of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; 5Department of Anatomical Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; 6Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; 7Cancer Research Center, Semnan University of Medical Sciences, Semnan, IranCorrespondence: Ali Rashidy-PourResearch Center of Physiology, University of Medical Sciences, Semnan 15131-38111, IranTel +98-9121140221Email Rashidy-Pour@semums.ac.irObjective: The amygdala structural and functional abnormalities have been implicated in numerous neuropsychiatric and neurodevelopmental disorders. Given the important role of the amygdala in stress responses and the susceptibility of the females to adolescent stress, the present study investigated the beneficial effects of Spirulina platensis microalgae (SP) as a neuroprotective supplement against adolescent stress-induced oxidative stress, brain-derived neurotrophic factor (BDNF) alterations, molecular and morphological remodeling in the basolateral amygdala (BLA) of adult female rats.Methods: During the adolescent period (PNDs 30– 40) rats were subjected to restraint stress (2 h/day for 10 days). Then, the animals were subjected to 15 days treatment (PNDs 41– 55) with SP (200 mg/kg/day) followed by biochemical (BDNF and stress oxidative markers), molecular (BDNF and its receptor tropomyosin receptor kinase B [TrkB] mRNA expression), and morphological (dendritic length and spines) assessments in the BLA.Results: The study revealed that adolescent stress decreased BDNF levels and reduced apical dendritic length and branch points of pyramidal neurons in the BLA. In addition, chronic stress significantly increased oxidative stress parameters and decreased BDNF and TrkB mRNA expression in the BLA. Treatment with SP alleviated both biochemical, molecular, and neuroanatomical deficits that induced by adolescent stress.Conclusion: Our findings provide important evidence that SP as a non-pharmacological intervention during adolescent period can protect against chronic stress-induced neuroanatomical biochemical, and molecular deficits in adulthood, and thus, reduce stress-related disorders.Keywords: adolescent stress, amygdala, BDNF, dendritic remodeling, Spirulina, oxidative markers

• Adolescent stress induced PTSD-like behaviors in adulthood. • Adolescent stress enhanced HPA negative feedback inhibition in adulthood. • Adolescent stress increased GR expression in the hypothalamus and hippocampus. • Environmental enrichment ameliorated behavioral abnormalities and HPA dysregulation. Exposure to early stressful events increases susceptibility to post-traumatic stress disorder (PTSD) in adulthood, in which the hypothalamic-pituitary-adrenal (HPA) axis plays a crucial role. Studies have found that environmental enrichment (EE) mitigates the detrimental outcomes of early adversity. However, the HPA-related mechanism remains unclear. In this study, we used the single prolonged stress (SPS) paradigm to explore the long-term effects of early adolescent stress on behavior, HPA axis activity, as well as expression levels of the glucocorticoid receptor (GR), mineralocorticoid receptor (MR), corticotropin-releasing hormone receptor 1 (CRF1R) and CRF2R in the hypothalamus and hippocampus. Meanwhile, the protective effects of EE intervention were examined. We found that adult male rats exposed to adolescent stress showed reduced locomotor activity, increased anxiety-like behaviors, enhanced contextual fear memory, elevated basal plasma ACTH levels, and enhanced HPA negative feedback inhibition, as indicated by decreased plasma ACTH levels in the dexamethasone suppression test (DST). Furthermore, EE normalized the behavioral abnormalities and enhanced HPA negative feedback in stressed rats, possibly through down-regulating GR expression in the hippocampus and hypothalamus. These findings suggested that EE could ameliorate adolescent stress-induced PTSD-like behaviors and aberrant reprogramming of the HPA axis, reducing the risk of developing PTSD in adulthood. [ABSTRACT FROM AUTHOR]

Adolescence is a time of intense cortical development and a period of heightened sensitivity to insult. To determine how sex affects the short- and long-term outcomes of early-adolescent stress exposure, we subjected prepubescent (postnatal day 30) male and female mice to repeated multiple concurrent stressors (RMS). In the posterior parietal cortex (PPC), RMS caused the elimination of excitatory synapses in deeper layers while inhibitory synapse density was predominantly diminished in superficial layers. These short-term effects coincided with reduced visuo-spatial working memory and were similar in both sexes. The loss of excitatory synapses and impaired working memory persisted in males past a 30-day recovery period. In contrast, we observed a remarkable recovery of excitatory transmission and behavioral performance in females. Inhibitory synapse density recovered in both sexes. We have also observed a late onset anxiety phenotype in RMS exposed females that was absent in males. Overall, our results indicate that there are marked sex differences in the long-term effects of prepubescent stress on cortical synapses and behavior.

Stress during adolescence has profound effects on the onset and severity of substance use later in life. However, not everyone with adverse experiences during this period will go on to develop a substance use disorder in adulthood, and the factors that alter susceptibility to substance use remain unknown. Here, we investigated individual differences in response to stress and drugs of abuse using our selectively bred high-responder (bHR) and low-responder (bLR) rats. These animals model extremes of temperamental tendencies and differ dramatically in both stress responsiveness and addiction-related traits. The present study investigated how environmental interventions in the form of a chronic variable stress (CVS) regimen in early adolescence interact with the bHR/bLR phenotype to alter behavioral sensitization to cocaine in adulthood. We also determined whether accumbal dopamine signaling is involved in the interaction of stress history and cocaine by assessing the mRNA levels of dopamine D1 (D1R) and D2 (D2R) receptors. Our results showed that CVS history alone had enduring and phenotype-specific effects on accumbal dopamine signaling. Importantly, adolescent stress had opposing effects in the two lines- decreasing the locomotor response to cocaine challenge in bHRs but increasing this measure in bLRs. Moreover, these opposing effects on cocaine sensitivity following adolescent CVS were accompanied by parallel effects in the accumbal dopamine system, with prior stress and cocaine exposure interacting to decrease D2R mRNA in bHRs but increase it in bLRs. Overall, these findings indicate that environmental challenges encountered in adolescence interact with genetic background to alter vulnerability to cocaine later in life. Stress experienced during adolescence affects the onset and severity of drug dependence later in life. However, not everyone with adverse experiences during this period will go on to develop SUD in adulthood. Using a rat model of innate differences in emotional reactivity, this study shows that the interplay between individual temperament and previous experience of adolescent stress/trauma determines whether an individual will be vulnerable or resilient to develop SUDs later in life. In addition, the present study shows that the dopamine D2 receptor in the brain's reward center, nucleus accumbens, may be implicated in this interplay. [ABSTRACT FROM AUTHOR]

A broad stream of research strives to understand stress directly or indirectly resulting from the use of information and communication technology (ICT), commonly referred to as technostress. A group at high risk of suffering from the consequences of technostress is adolescents because they grow up using ICT daily and are still developing their identity, acquiring mental strength, and adopting essential social skills. Our research combines a qualitative and a quantitative study and contributes to an understanding of what strategies adolescents use to cope with the demands of ICT use. In qualitative workshops with adolescents, we collect 30 coping responses in five categories. A subsequent quantitative study finds gender- and age-related differences in adolescents' perception of technostress and concludes that adolescents as a group activate a broad portfolio of coping responses. Exploratory factor analysis reveals five factors underlying adolescents' activation of coping responses: Avoid Stressful ICT, Follow the Rules, Use ICT Consciously, Contain Negative Emotions, and Acquire ICT. We find that the coping responses related to the Avoid Stressful ICT factor are significantly more common among girls than boys and derive that adolescents who own more devices might have a lower tendency to Follow the Rules. A joint analysis of coping responses and technostress creators reveals that, not surprisingly, coping increases with higher intensity of technostress, but some coping responses break out of this pattern. With this research, we contribute to the theoretical and empirical understanding of an important phenomenon associated with digitalization's dark sides (technostress coping) in an important yet understudied population (adolescents 10–17 years of age). Future research may build on our work and investigate additional parameters determining differences in adolescents' coping behavior. [ABSTRACT FROM AUTHOR]

• Behavioral sensitization to cocaine was enhanced by GXE. • PDE4 activity was increased in the nucleus accumbens of GXE mice. • Effects of rolipram on the phophrylation of DARPP-32 and GluA1 was enhanced by GXE. • Rolipram inhibited enhanced behavioral sensitization to cocaine in GXE mice. Development of drug addictive behaviors is modulated by both genetic and environmental risk factors. However, the molecular mechanisms remain unknown. To address the role of adolescent stress in the development of drug addiction, we combined a transgenic mouse model in which a putative dominant-negative form of DISC1 under expressional control of the prion protein promoter is used as a genetic risk factor and adolescent social isolation stress as a gene-environmental interaction (GXE). Repeated cocaine exposure induced greater locomotion in the GXE group than in the other groups. In a conditioned place preference (CPP) test, GXE mice exhibited a significant place preference to the cocaine-conditioned area compared with the other groups. In the nucleus accumbens (NAc) of GXE mice, we found increased enzyme activity of phosphodiesterase-4 (PDE4), predominantly located in NAc D2-receptor-expressing neurons, and enhanced effects of the PDE4 inhibitor rolipram, but not the D1 agonist SKF81297, on the phosphorylation of DARPP-32 and GluA1 at PKA sites. Rolipram injection before cocaine exposure completely inhibited cocaine-induced hyperlocomotion and CPP in the GXE group. These results indicate that GXE enhances sensitivity to repeated cocaine exposure via an increase in PDE4 activity in NAc D2-recptor-expressing neurons, leading to the development of cocaine addictive behaviors. [ABSTRACT FROM AUTHOR]

Background and Hypothesis: Redox dysregulation has been proposed as a convergent point of childhood trauma and the emergence of psychiatric disorders, such as schizophrenia (SCZ). A critical region particularly vulnerable to environmental insults during adolescence is the ventral hippocampus (vHip). However, the impact of severe stress on vHip redox states and their functional consequences, including behavioral and electrophysiological changes related to SCZ, are not entirely understood., Study Design: After exposing adolescent animals to physical stress (postnatal day, PND31-40), we explored social and cognitive behaviors (PND47-49), the basal activity of pyramidal glutamate neurons, the number of parvalbumin (PV) interneurons, and the transcriptomic signature of the vHip (PND51). We also evaluated the impact of stress on the redox system, including mitochondrial respiratory function, reactive oxygen species (ROS) production, and glutathione (GSH) levels in the vHip and serum., Study Results: Adolescent-stressed animals exhibited loss of sociability, cognitive impairment, and vHip excitatory/inhibitory (E/I) imbalance. Genome-wide transcriptional profiling unveiled the impact of stress on redox system- and synaptic-related genes. Stress impacted mitochondrial respiratory function and changes in ROS levels in the vHip. GSH and glutathione disulfide (GSSG) levels were elevated in the serum of stressed animals, while GSSG was also increased in the vHip and negatively correlated with sociability. Additionally, PV interneuron deficits in the vHip caused by adolescent stress were associated with oxidative stress., Conclusions: Our results highlight the negative impact of adolescent stress on vHip redox regulation and mitochondrial function, which are partially associated with E/I imbalance and behavioral abnormalities related to SCZ., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

• Glucocorticoid receptor (GR) expression in the amygdala is altered by prenatal alcohol exposure (PAE). • PAE effects on amygdala GR expression were more pronounced during adolescence. • GR expression in the mPFC is altered by adolescent stress. • Adolescent stress effects on mPFC GR expression were more pronounced in adulthood. The dense expression of glucocorticoid receptors (GR) within the amygdala, medial prefrontal cortex (mPFC) and paraventricular nucleus of hypothalamus (PVN) mediates many aspects of emotional and stress regulation. Importantly, both prenatal alcohol exposure (PAE) and adolescent stress are known to induce emotional and stress dysregulation. Little is known, however, about how PAE and/or adolescent stress may alter the expression of GR in the amygdala, mPFC, and PVN. To fill this gap, we exposed PAE and control adolescent male and female rats to chronic mild stress (CMS) and assessed GR mRNA expression in the amygdala, mPFC, and PVN immediately following stress or in adulthood. We found that the effects of PAE on GR expression were more prevalent in the amygdala, while effects of adolescent stress on GR expression were more prevalent in the mPFC. Moreover, PAE effects in the amygdala were more pronounced during adolescence and adolescent stress effects in the mPFC were more pronounced in adulthood. GR expression in the PVN was affected by both PAE and adolescent stress. Finally, PAE and/or adolescent stress effects were distinct between males and females. Together, these results suggest that PAE and adolescent CMS induce dynamic alterations in GR expression in the amygdala, mPFC, and PVN, which manifest differently depending on the brain area, age, and sex of the animal. Additionally, these data indicate that PAE-induced hyperresponsiveness to stress and increased vulnerability to mental health problems may be mediated by different neural mechanisms depending on the sex and age of the animal. [ABSTRACT FROM AUTHOR]

Chronic exposure to stress during adolescent period has been demonstrated to impair cognitive functions and the dendritic morphology of pyramidal neurons in the rat hippocampal CA3 area. The present study investigated the combined protective effects of Spirulina platensis (SP), a supplement made from blue-green algae with neuroprotective properties, voluntary exercise (EX) and environmental enrichment (EE) against cognitive deficits, alternations in hippocampal BDNF levels, and abnormal neuronal remodeling in adult female rats (PND 60) induced by exposure to chronic restraint stress during adolescent period (PND 30–40). Rats were exposed to restraint stress (2 h/day for 10 days, PND 30–40). Then, the animals were subjected to treatment with SP (200 mg/kg/day), EX, EE and the combined treatments (SP + EX, and SP + EE) between PND 41 and 55 of age. Following the interventions, spatial learning and memory, passive avoidance performance, hippocampal dendritic morphology and BDNF levels were assessed. Results showed that plasma corticosterone levels increased at PND 40 and remained elevated at PND 55 and 70 in the stressed rats. Stressed rats showed deficits in spatial learning and memory and passive avoidance performance, decreased BDNF levels in the hippocampus, and reduced apical dendritic length and branch points of the CA3 pyramidal neurons. These deficits were alleviated by the SP, EX and EE, and the combined treatments, which accompanied with a decline in serum corticosterone in stressed animals. Some treatments even enhanced cognitive functions, and BDNF levels and neuroanatomical remodeling in the hippocampus of non-stressed animals. Our findings provide important evidences that physical activity, exposure to EE, and the SP treatment during adolescent period can protect against adolescent stress induced behavioral, biochemical and neuroanatomical impairments in adulthood. • Adolescent stress induces cognitive and hippocampal BDNF in adulthood. • Adolescent stress causes dendritic retraction in CA3 pyramidal neurons in adulthood. • Spirulina platensis (SP) treatment, enriched environment (EE) and exercise ameliorated stress effects. • Combined treatments were more effective against stress effects on BDNF and dendritic morphology. • Using SP, EE and physical activity are efficient therapeutic ways against adolescent stress. [ABSTRACT FROM AUTHOR]

Increasing evidence suggests that exposure to chronic stress during adolescent period may lead to behavioral and neuronal morphology deficits in adulthood. This study examined whether crocin, the main active saffron constituent, and voluntary exercise, alone or combined, could prevent the detrimental influences of chronic restraint stress during adolescent (postnatal days, PND, 30–40) on behavioral and morphological deficits in adult (PND60) male rats. Results showed that plasma corticosterone levels increased at PND40, but not PND60 in stressed rats. Moreover, stressed rats demonstrated enhanced anxiety levels and depression like behaviors in adulthood. These behavioral abnormalities were accompanied by a decline in apical dendritic length in both infralimbic and prelimbic regions and dendritic branches in infralimbic region of the prefrontal cortex. Treatment with crocin, exposure to wheel running activity, and the combined interventions alleviated both behavioral and morphological deficits induced by adolescent stress. Moreover, these treatments exerted positive neuronal morphological effects in the prefrontal cortex in non-stressed animals. Our findings provide important evidences that exercise as a non-pharmacological intervention and crocin treatment during pre-pubertal period can protect against adolescent stress induced behavioral and morphological abnormalities in adulthood. [ABSTRACT FROM AUTHOR]

Objective(s): The purpose of this paper is to report study findings related to themes of stress and coping strategies experienced by non‐reservation‐based Native American adolescents as expressed by their written stories. Design: A qualitative descriptive narrative approach was used to capture written stories of stress. Analysis: Key concepts and themes were identified by using the consensual qualitative research (CQR) approach from the participant's stories of stress. Results: Written stories of stress were collected and analyzed from a convenience sample of 179 Native American adolescent's ages 13–18‐years old. Five major themes related to experiences of stress emerged from the stories including (a) relationships, (b) responsibilities, (c) socioeconomic status, (d) negative coping strategies, and (e) positive coping through Native‐Reliance. Conclusion: The findings reveal the importance of understanding the types and response to stress experienced by Native American adolescents. [ABSTRACT FROM AUTHOR]

Abstract Childhood and adolescent adversity are associated with a wide range of psychiatric disorders, including an increased risk for substance abuse. Despite this, the mechanisms underlying the ability of chronic stress during adolescence to alter reward signaling remains largely unexplored. Understanding how adolescent stress increases addiction-like phenotypes could inform the development of targeted interventions both before and after drug use. The current study examined how prolonged isolation stress, beginning during adolescence, affected behavioral and neuronal underpinnings to the response to cocaine in male and female mice. Adolescent-onset social isolation did not alter the ability of mice to learn an operant response for food, nor influence food self-administration or motivation for food on a progressive ratio schedule. However, male and female social isolation mice exhibited an increase in motivation for cocaine and cocaine seeking during a cue-induced reinstatement session. Additionally, we demonstrated that adolescent-onset social isolation increased cocaine-induced neuronal activation, as assessed by c-Fos expression, within the nucleus accumbens core and shell, ventral pallidum, dorsal bed nucleus of the stria terminalis, lateral septum and basolateral amygdala. Taken together, the present studies demonstrate that social isolation stress during adolescence augments the behavioral responses to cocaine during adulthood and alters the responsiveness of reward-related brain circuitry. [ABSTRACT FROM AUTHOR]

Background: Stress is an important variable of consequence, particularly in adolescence, a period of intense physical and psychological change. The measurement of stress in adolescence has been widely discussed, and a number of versions of the Adolescent Stress Questionnaire (ASQ) have been developed and validated. The present study sought to examine the psychometric properties (model fit, invariance, internal consistency, and construct validity) of the ASQ-S, which was recently developed in a Swedish context. Objective: The study was a secondary analysis of data gathered on the full ASQ. The ASQ-S retained nine of the ten ASQ scales, and a study in Swedish adolescents suggested that the scale was psychometrically valid, gender invariant, and that scores were internally consistent. This is the first study to examine the properties of the ASQ-S in an English-speaking population. Participants were high school children in the UK (N = 610, 61.0% girls) from school year 8 through 12. Results: Confirmatory factor analysis (CFA) revealed that the nine factor solution fit the data well (x2s-b(288) = 751.60, CFI = 0.958, TLI = 0.948, SRMR = 0.040, RMSEA = 0.051 [90% CI = 0.047, 0.056]), and that scores were gender, school type (academic versus comprehensive), and school stage (junior versus middle high school) invariant. The nine scales correlated negatively with academic, social, and emotional self-efficacy scores, and self-esteem scores, to varying degrees. Girls reported higher stress levels than boys in six of the nine scales. A regression analysis, adjusted for gender and year in school, suggested that only the stress of peer pressure (negatively) was significantly related to adolescent alcohol use. Conclusions: Overall this study suggests that the ASQ-S could be a valid measure of adolescent stress, although concerns remain regarding the convergent validity of scale scores. [ABSTRACT FROM AUTHOR]

A better understanding about prerequisites of health and well-being in adolescents is important to prevent chronicity and comorbidities of stress and to improve health promotion in this group. For this purpose, useful instruments are required. The Perceived Stress Questionnaire (PSQ) is developed for research, with an emphasis on predictive validity. The PSQ comprises different components of stress, and the instrument might be useful in studying prerequisites and predictors of health and well-being in adolescents. However, the instrument has not been evaluated in Norwegian psychosomatic populations and in adolescents. Moreover, the factor structure of the PSQ seems to vary between populations, and invariance across gender and concurrent validity regarding mindfulness are not previously tested. The objective of this study were to evaluate the psychometric properties of the Norwegian version of the PSQ in adolescents, including evaluate the fit of previously proposed PSQ-models in females and males and test measurement invariance across gender. Concurrent validity with respect to mindfulness (Mindful Attention Awareness Scale- Adolescent [MAAS-A]) was preliminary evaluated. Confirmatory factor analysis for each previously proposed model was conducted, separately for females and males. Multi-group factor analyses were performed to test measurement invariance of the different PSQ-models across gender. The associations between the PSQ and the MAAS-A and inter-scale correlations were preliminary evaluated. Preceding the data collection and main analyses, the instruments were translated to Norwegian following standardized procedures. The participants in study were Norwegian adolescents aged 15–16 years (N = 524). The overall PSQ performance seemed to correspond to previous findings, and internal consistency was supported across gender. A four-factor model of the PSQ showed best fit to the data in both females and males and configural and metric invariance seemed supported. Full scalar invariance was not supported for the four-factor model, implying that cross-group comparisons (between females and males) on latent means may be uncertain and must be interpreted with caution. Concurrent validity with respect to mindfulness (MAAS-A) was preliminary supported. Further studies might be needed to confirm the findings from this study.

Disrupted-in-schizophrenia 1 (DISC1) is a strong candidate susceptibility gene for a spectrum of neuropsychiatric diseases including schizophrenia, bipolar disorder and major depression, all of which are thought to result from interactions between gene mutations and environmental risk factors such as influenza, trauma and stress. Adolescence is a key period susceptible to stress and stress-related mental illnesses. In a previous study, we found that although DISC1 L100P point mutation mice shows object recognition deficits, their sociability and social memory are relatively normal. Therefore, in this article, we investigated whether the interaction between adolescent stress and DISC1 L100P point mutation affects adult social memory, and we explored the underlying mechanisms. We found that adolescent stress (isolation from 5 weeks to 8 weeks of age) specifically impaired social memory of adult DISC1 L100P mice but not that of WT littermates, which could be rescued by administration of atypical antipsychotic drug clozapine. On the other hand, it did not induce anxiety or depression in adult mice. Adolescent isolation exacerbated adult neurogenesis deficits in the hippocampus of DISC1 L100P mice, while it had no effect on WT mice. In addition, we found that adolescent isolation led to long lasting changes in synaptic transmission and plasticity in the hippocampal circuits, some of which are specific for DISC1 L100P mice. In summary, we identified here the specific interaction between genetic mutation (DISC1 L100P) and adolescence social stress that damages synaptic function and social memory in adult hippocampal circuits.Highlights–Adolescent isolation (from 5 weeks to 8 weeks of age) impairs adult social memory when combined with DISC1 L100P point mutation.–Adolescent isolation exacerbates adult neurogenesis deficit in the hippocampus of L100P mice but has no similar effect on WT mice.–Adolescent isolation causes long lasting changes in synaptic transmission and plasticity of the hippocampal network in DISC1 L100P mice.

Many individuals sporadically and circumstantially sample addictive drugs, yet few become addicted. The individual vulnerabilities underlying the development of addiction are not well understood. Correlational findings show that early life adversity is associated with a greater propensity to develop drug addiction. However, the mechanisms by which early life adversity increases addiction vulnerability are unknown. Separate lines of research have found that several traits are associated with addiction. Here, we examined the effects of early life adversity on addiction-related traits in adulthood. We weaned male and female Sprague-Dawley rats (postnatal day – PND21) and randomly assigned them to either a non-adversity group (N-ADV) or an adversity group (ADV). ADV rats experienced adversity from PND 21-35, they were: a) singly housed, b) food restricted for 12 h/day, c) subjected to forced-swim sessions, and d) restrained and exposed to predator odour (1 h). As adults, rats were tested for impulsivity, anxiety-like behaviour, novelty preference, and attribution of incentive salience to a reward cue. ADV rats showed enhanced novelty preference and attributed greater incentive value to a reward cue. Compared to N-ADV rats, a greater proportion of ADV rats expressed multiple addiction risk traits. Furthermore, fewer ADV rats expressed no addiction risk traits. This effect was most evident in female ADV rats. [ABSTRACT FROM AUTHOR]

Highlights • Adolescent stress increases anxiety-like behaviors. • Anxiety-like behaviors decrease from adolescence to adulthood in control animals. • Adolescent stress impacts the prefrontal cortex in a sex- and age-specific manner. • Parvalbumin cell counts correlate with anxiety behavior in adult control females. Abstract Adolescence is a window of vulnerability to environmental factors such as chronic stress that can disrupt brain development and cause long-lasting behavioral dysfunction, as seen in disorders like depression, anxiety, and schizophrenia. There are also sex differences in the prevalence of these disorders across the lifespan. However, the mechanisms of how adolescent stress contributes to neuropsychiatric phenotypes are not well understood, nor are the mediating effects of sex. We hypothesize that adolescent stress disrupts the γ-aminobutyric acid (GABA) system in the prefrontal cortex (PFC) in a sex-specific manner, as this system matures during adolescence and plays an important role in cognitive and emotional functioning. We exposed male and female mice to unpredictable chronic mild stress (UCMS) during adolescence (post-natal day [PND] 28–42). One cohort underwent testing for PFC-related behavioral and molecular changes 24 h following the cessation of stress (late adolescence); a separate cohort was tested approximately 2.5 weeks after the end of UCMS (adulthood). We observed an age-related decline in anxiety-like behaviors in control mice, while mice stressed in adolescence showed elevated anxiety-like behaviors in both adolescence and adulthood. PFC-dependent cognitive functioning was also impaired in adult males stressed in adolescence. Adolescent stress disrupted expression patterns of parvalbumin (PV) and perineuronal nets (PNNs) in the PFC, as well as NMDA receptor subunit composition, in a sex- and age-specific manner. The findings presented here contribute to understanding how adolescent stress may lead to neuropsychiatric disorders such as anxiety by disrupting the development of the PFC and emotional behaviors. [ABSTRACT FROM AUTHOR]

A better understanding about prerequisites of health and well-being in adolescents is important to prevent chronicity and comorbidities of stress and to improve health promotion in this group. For this purpose, useful instruments are required. The Perceived Stress Questionnaire (PSQ) is developed for research, with an emphasis on predictive validity. The PSQ comprises different components of stress, and the instrument might be useful in studying prerequisites and predictors of health and well-being in adolescents. However, the instrument has not been evaluated in Norwegian psychosomatic populations and in adolescents. Moreover, the factor structure of the PSQ seems to vary between populations, and invariance across gender and concurrent validity regarding mindfulness are not previously tested. The objective of this study were to evaluate the psychometric properties of the Norwegian version of the PSQ in adolescents, including evaluate the fit of previously proposed PSQ-models in females and males and test measurement invariance across gender. Concurrent validity with respect to mindfulness (Mindful Attention Awareness Scale- Adolescent [MAAS-A]) was preliminary evaluated. Confirmatory factor analysis for each previously proposed model was conducted, separately for females and males. Multi-group factor analyses were performed to test measurement invariance of the different PSQ-models across gender. The associations between the PSQ and the MAAS-A and inter-scale correlations were preliminary evaluated. Preceding the data collection and main analyses, the instruments were translated to Norwegian following standardized procedures. The participants in study were Norwegian adolescents aged 15–16 years (N = 524). The overall PSQ performance seemed to correspond to previous findings, and internal consistency was supported across gender. A four-factor model of the PSQ showed best fit to the data in both females and males and configural and metric invariance seemed supported. Full scalar invariance was not supported for the four-factor model, implying that cross-group comparisons (between females and males) on latent means may be uncertain and must be interpreted with caution. Concurrent validity with respect to mindfulness (MAAS-A) was preliminary supported. Further studies might be needed to confirm the findings from this study. [ABSTRACT FROM AUTHOR]

Disrupted-in-schizophrenia 1 (DISC1) is a strong candidate susceptibility gene for a spectrum of neuropsychiatric diseases including schizophrenia, bipolar disorder and major depression, all of which are thought to result from interactions between gene mutations and environmental risk factors such as influenza, trauma and stress. Adolescence is a key period susceptible to stress and stress-related mental illnesses. In a previous study, we found that although DISC1 L100P point mutation mice shows object recognition deficits, their sociability and social memory are relatively normal. Therefore, in this article, we investigated whether the interaction between adolescent stress and DISC1 L100P point mutation affects adult social memory, and we explored the underlying mechanisms. We found that adolescent stress (isolation from 5 weeks to 8 weeks of age) specifically impaired social memory of adult DISC1 L100P mice but not that of WT littermates, which could be rescued by administration of atypical antipsychotic drug clozapine. On the other hand, it did not induce anxiety or depression in adult mice. Adolescent isolation exacerbated adult neurogenesis deficits in the hippocampus of DISC1 L100P mice, while it had no effect on WT mice. In addition, we found that adolescent isolation led to long lasting changes in synaptic transmission and plasticity in the hippocampal circuits, some of which are specific for DISC1 L100P mice. In summary, we identified here the specific interaction between genetic mutation (DISC1 L100P) and adolescence social stress that damages synaptic function and social memory in adult hippocampal circuits. Highlights – Adolescent isolation (from 5 weeks to 8 weeks of age) impairs adult social memory when combined with DISC1 L100P point mutation. – Adolescent isolation exacerbates adult neurogenesis deficit in the hippocampus of L100P mice but has no similar effect on WT mice. – Adolescent isolation causes long lasting changes in synaptic transmission and plasticity of the hippocampal network in DISC1 L100P mice. [ABSTRACT FROM AUTHOR]

Background: Stress during the adolescent period influences postnatal maturation and behavioral patterns in adulthood. Adolescent stress-induced molecular and functional changes in neurons are the key clinical features of psychiatric disorders including schizophrenia. Objective: In the present study, we exposed genetically vulnerable mice to isolation stress to examine the molecular changes in the glutamatergic system involving N-methyl- d-aspartate (NMDA) receptors via dopaminergic disturbance in the prefrontal cortex (PFc). Results: We report that late adolescent stress in combination with Disrupted-in-Schizophrenia 1 ( DISC1) genetic risk elicited alterations in glutamatergic neurons in the PFc, such as increased expression of glutamate transporters, decreased extracellular levels of glutamate, decreased concentration of d-serine, and impaired activation of NMDA-Ca/calmodulin kinase II signaling. These changes resulted in behavioral deficits in locomotor activity, forced swim, social interaction, and novelty preference tests. The glutamatergic alterations in the PFc were prevented if the animals were treated with an atypical antipsychotic drug clozapine and a dopamine D1 agonist SKF81297, which suggests that the activation of dopaminergic neurons is involved in the regulation of the glutamatergic system. Conclusion: Our results suggest that adolescent stress combined with dopaminergic abnormalities in the PFc of genetically vulnerable mice induces glutamatergic disturbances, which leads to behavioral deficits in the young adult stage. [ABSTRACT FROM AUTHOR]

The glucocorticoid hypothesis suggests that overexposure to stress may cause permanent upregulation of cortisol. Stress in youth may therefore influence cortisol levels even in older age. Using data from the 6-Day Sample, we investigated the effects of high stress in childhood, adolescence and early adulthood – as well as individual variables contributing to these measures; parental loss, social deprivation, school and home moves, illness, divorce and job instability – upon cortisol levels at age 77 years. Waking, waking +45 min (peak) and evening salivary cortisol samples were collected from 159 participants, and the 150 who were not using steroid medications were included in this study. After correcting for multiple comparisons, the only significant association was between early-adulthood job instability and later-life peak cortisol levels. After excluding participants with dementia or possible mild cognitive impairment, early-adulthood high stress showed significant associations with lower evening and mean cortisol levels, suggesting downregulation by stress, but these results did not survive correction for multiple comparisons. Overall, our results do not provide strong evidence of a relationship between stress in youth and later-life cortisol levels, but do suggest that some more long-term stressors, such as job instability, may indeed produce lasting upregulation of cortisol, persisting into the mid-to-late seventies. [ABSTRACT FROM PUBLISHER]

In view of the significance of emotional intelligence in the lives of human beings, the present study investigated the preventive, therapeutic, and moderating effects of emotional intelligence on adolescent stress. It also examined the impact of emotional intelligence on the academic achievement of adolescent students. The participants comprised of 2,060 adolcsccnts belonging to the age rangcof 14 to 19 years, selected randomly from 31 educational institutions in Emakulam district of Kerala. Emotional intelligence was found to be significantly related to adolescent stress (inverse relationship) and psychological well-being, thus supporting the preventive and therapeutic effects of emotional intelligence on adolescent stress. However, significant moderating effect was observed for only one of the ten stress dimensions. Adolescents who have high academic achievement were found to have higher levels of emotional intelligence. [ABSTRACT FROM AUTHOR]

Being bullied during adolescence is associated with later mental illnesses characterized by deficits in cognitive tasks mediated by prefrontal cortex (PFC) dopamine (DA). Social defeat of adolescent male rats, as a model of teenage bullying victimization, results in medial PFC (mPFC) dopamine (DA) hypofunction in adulthood that is associated with increased drug seeking and working memory deficits. Increased expression of the DA transporter (DAT) is also seen in the adult infralimbic mPFC following adolescent defeat. We propose the functional consequence of this increased DAT expression is enhanced DA clearance and subsequently decreased infralimbic mPFC DA availability. To test this, in vivo chronoamperometry was used to measure changes in accumulation of the DA signal following DAT blockade, with increased DAT-mediated clearance being reflected by lower DA signal accumulation. Previously defeated rats and controls were pre-treated with the norepinephrine transporter (NET) inhibitor desipramine (20 mg/kg, ip.) to isolate infralimbic mPFC DA clearance to DAT, then administered the selective DAT inhibitor GBR-12909 (20 or 40 mg/kg, sc.). Sole NET inhibition with desipramine produced no differences in DA signal accumulation between defeated rats and controls. However, rats exposed to adolescent social defeat demonstrated decreased DA signal accumulation compared to controls in response to both doses of GBR-12909, indicating greater DAT-mediated clearance of infralimbic mPFC DA. These results suggest that protracted increases in infralimbic mPFC DAT function represent a mechanism by which adolescent social defeat stress produces deficits in adult mPFC DA activity and corresponding behavioral and cognitive dysfunction. [ABSTRACT FROM AUTHOR]

Abnormal activity of stress hormone (hypothalamic–pituitary–adrenal [HPA]), and gonadal hormone (hypothalamic–pituitary–gonadal [HPG]) systems is reported following prenatal alcohol exposure (PAE). PAE increases vulnerability of brain regions involved in regulation of these systems to stressors or challenges during sensitive periods of development, such as adolescence. In addition, HPA and HPG functions are linked to higher order functions such as executive function (EF), with dysregulation of either system adversely affecting EF processes, including attention and response inhibition, that influence cognition. However, how HPA and HPG systems interact to influence cognitive performance in individuals with an FASD is not fully understood. To investigate, we used a rat model of moderate PAE. Adolescent female PAE and control offspring were exposed to 10 days of chronic mild stress (CMS) and cognitive function was assessed on the radial arm maze (RAM) in adulthood. On the final test day, animals were sacrificed, with blood collected for hormone analyses, and vaginal smears taken to assess estrus stage at the time of termination. Analyses showed that adolescent CMS significantly increased levels of CORT and RAM errors during proestrus in adult PAE but not control females. Moreover, CORT levels were correlated with estradiol levels and with RAM errors, but only in PAE females, with outcome dependent on adolescent CMS condition. These results suggest that PAE increases sensitivity to the influences of stress and gonadal hormones on cognition, and thus, in turn, that HPA and HPG dysregulation may underlie some of the deficits in executive function described previously in PAE females. [ABSTRACT FROM AUTHOR]

Depression, anxiety and stress are common problems among adolescents. Teaching young people coping strategies in school-based intervention programs is one promising approach hoped to remedy the negative consequences of distress in adolescence. The aim of the two pilot studies was to examine the effect of a brief intervention based on the principles of Acceptance and Commitment Therapy (ACT) on depressive symptomatology (Australian study, N = 66) and stress (Swedish study, N = 32) among adolescents screened for psychosocial problems in school settings. In both studies, subjects were assigned to receive the ACT-group-intervention, or a control intervention featuring individual support from the school health care. The Australian study was a planned comparison, with random allocation for girls, plus one replication of a boys group. The Swedish study used a randomized controlled design. The ACT-intervention was an 8-session manualized group program. The Australian study showed significant reductions in depressive symptoms with a large effect, and significant reductions in psychological inflexibility with a medium effect when compared to the control group who received standard care. In the Swedish study, the ACT-intervention group, when compared to the control group, reported significantly lower levels of stress with a large effect size, and marginally significant decrease of anxiety, and marginally significant increased mindfulness skills. Taken together, the ACT-intervention seems to be a promising intervention for reducing stress and depressive symptoms among young adolescents in school and should be tested in full-sized studies. Limitations of these two pilots include small samples. [ABSTRACT FROM AUTHOR]

In humans, exposure to stress during development is associated with structural and functional alterations of the prefrontal cortex (PFC), amygdala (AMY), and hippocampus (HC) and their circuits of connectivity, and with an increased risk for developing major depressive disorder particularly in carriers of the short (s) variant of the serotonin transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR). Although changes in these regions are found in carriers of the s allele and/or in depressed patients, evidence for a specific genotype x developmental stress effect on brain structure and function is limited. Here, we investigated the effect of repeated stress exposure during adolescence in mice with partial knockout of the 5-HTT gene (HET) versus wildtype (WT) on early-adulthood behavioral measures and brain structure (using magnetic resonance imaging) relevant to human major depression. Behaviorally, adolescent stress (AS) increased anxiety and decreased activity and did so to a similar degree in HET and WT. In a probabilistic reversal learning task, HET-AS mice achieved fewer reversals than did HET-No-AS mice. 5-HTT genotype and AS were without effect on corticosterone stress response. In terms of structural brain differences, AS reduced the volume of two long-range white matter tracts, the optic tract and the cerebral peduncle, in WT mice specifically. In a region-of-interest analysis, AS was associated with increased HC volume and HET genotype with a decreased frontal lobe volume. In conclusion, we found that 5-HTT and AS genotype exerted long-term effects on behavior and development of brain regions relevant to human depression.

Abstract: Adolescence is a period of transition from childhood to adulthood that involves the maturation of social and cognitive behavior. The activation of the stress system during this phase can lead to long-lasting adverse effects. We aimed to verify whether the nature and duration of stressors applied in adolescent female and male rats would alter their exploratory behavior and stress responses as adults. Wistar rats on day P26 were divided into groups that were subjected to 1 (acute) or 7 (chronic) insulin or lipopolysaccharide (LPS) injections or restraint stress for 1h. At P60, the rats were subjected to the elevated plus-maze, and at P61, they were subjected to 30min of restraint stress after which plasma samples and brains were collected. LPS acute injection promoted anxiolytic effects in male adults. Acute LPS treatment and acute or chronic restraint induced anxiolytic behavior in female adults. The administration of adolescent chronic stimuli to males decreased the adult plasma corticosterone (CORT) and progesterone levels after restraint. Adolescent acute restraint or LPS injection decreased the CORT response in female adults. The adult neuronal activation of the corticotrophin-releasing hormone and vasopressin on the paraventricular nucleus did not vary according to the type of adolescent stress or sex. Our results indicate that both adult behavior and the glucocorticoid stress response are affected differently in males versus females by adolescent stress. The duration of stressors had a greater effect on the CORT and progesterone response in males, whereas the nature of the stressor had a greater effect on exploratory behavior in females. [Copyright &y& Elsevier]

In humans, exposure to stress during development is associated with structural and functional alterations of the prefrontal cortex (PFC), amygdala (AMY), and hippocampus (HC) and their circuits of connectivity, and with an increased risk for developing major depressive disorder particularly in carriers of the short (s) variant of the serotonin transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR). Although changes in these regions are found in carriers of the s allele and/or in depressed patients, evidence for a specific genotype × developmental stress effect on brain structure and function is limited. Here, we investigated the effect of repeated stress exposure during adolescence in mice with partial knockout of the 5-HTT gene (HET) vs. wildtype (WT) on early-adulthood behavioral measures and brain structure [using magnetic resonance imaging (MRI)] relevant to human major depression. Behaviorally, adolescent stress (AS) increased anxiety and decreased activity and did so to a similar degree in HET and WT. In a probabilistic reversal learning task, HET-AS mice achieved fewer reversals than did HET-No-AS mice. 5-HTT genotype and AS were without effect on corticosterone stress response. In terms of structural brain differences, AS reduced the volume of two long-range white matter tracts, the optic tract (OT) and the cerebral peduncle (CP), in WT mice specifically. In a region-of-interest analysis, AS was associated with increased HC volume and HET genotype with a decreased frontal lobe volume. In conclusion, we found that 5-HTT and AS genotype exerted long-term effects on behavior and development of brain regions relevant to human depression. [ABSTRACT FROM AUTHOR]

Adolescence is a stressful time for many children. Changes in their environment or changes in social situations are some typical stressors that an adolescent child might encounter. Interactions with parents can also be stressors for a child. Previous research has shown that a risk factor for a parent using harsh parenting techniques is perceived control. Parents who have low perceived control are at a higher risk to engage in physical parenting techniques or child abuse. This study included 198 middle school students and their female parent or guardian pairs (296 total participants), with the adolescent participants ranging in age from 10- to 14-years-old. The adult participants were evaluated for their level of perceived control and the adolescent participants were evaluated for their level of perceived stress. Parents who perceived themselves as having a low amount of control over their child's behavior (low ACF), were linked with their child having a high level of perceived stress. This effect was found only for the 14-year-old participants. Implications of results and areas of further research are suggested. It is possible that as a child gets older and enters puberty, the parent of the child feels as if they are losing control over their child and, as a result, resort to more forceful parenting techniques to regain control. [ABSTRACT FROM AUTHOR]

Problem Statement: Adolescence is a stage of major growth and development in terms of significant cognitive, behavioral, psychological, and physiological changes. For adolescents, these developmental changes could be accompanied by stressful situations. Adolescents need to cope with these stressors successfully, yet the developmental period of adolescence involves an increase in interpersonal conflicts and negative emotions. Cognitive distortions play significant roles in exacerbating distress levels and negative conflict behavior. Consequently, the main focus of this study is an investigation into interpersonal cognitive distortions on adolescents' stress coping strategies. Purpose of Study: The aim of this study was to examine stress coping strategies and the interpersonal cognitive distortions of late adolescents. The relations between interpersonal cognitive distortions and stress coping strategies of late adolescents were tested as well. Methods: A total sample of 391 adolescents from public and private universities participated in this study. The descriptive statistical findings regarding the sub dimensions and t-test results indicating the difference between the means of males and females from "Interpersonal Cognitive Distortions" and "Stress coping Strategies with Stressful Experiences" scales were used. After this, findings related to interpersonal cognitive distortions and stress coping strategies were investigated by using path analysis in the research. Findings and Restdts: Results revealed that adolescents use the strategies of problem solving and seeking social support more than the strategies of self-blame, imagination, and avoidance. Also, it was found that the strongest predictors of interpersonal rejection among stress coping strategies are avoidance, self-blame, and seeking social support. In the unrealistic relationship expectation dimension, it can be seen that seeking social support, imagination, and avoidance strategies explain with a moderate effect. The interpersonal misperception dimension can only be explained by the self-blame strategy as a small effect. Conclusion and Recommendations: It was concluded that adolescents have a low level of interpersonal cognitive distortions and use problem solving and seeking social support strategies more than self-blame, imagination, and avoidance strategies. This means that adolescents use effective stress coping strategies and have low level interpersonal cognitive distortions. Another finding was that the strongest predictors of interpersonal rejection among stress coping strategies are avoidance, self-blame, and seeking social support. During the period of adolescence there are many stressful situations, such as future anxiety, career and occupation anxiety, romantic relationships, and the dimension change of relationships with family. Using effective stress coping strategies is important for overcoming stressful situations. Therefore, effective stress coping strategies should be taught to adolescents, especially for overcoming the stress they face in the period of adolescence. [ABSTRACT FROM AUTHOR]

Summary: Early life stress precipitates dysregulation of the hypothalamic–pituitary–adrenal (HPA) axis and this effect is most pronounced in females. The mechanisms that mediate female sensitivity to stress-induced HPA axis dysregulation are unknown. The purpose of this study was to determine whether sex moderates the effects of chronic adolescent stress on glucocorticoid receptor (GR) translocation and moderators of the GR system. Female adolescent rats with a history of chronic stress exposure demonstrated a delayed resolution of the plasma corticosterone response to an acute stressor and this delay was accompanied by attenuated GR translocation compared to control adolescent females. The chronic stress-induced phenotype in females was similar to the baseline phenotype in male adolescent rats. Conversely, the expression patterns of GR moderators/co-chaperones became more sexually dimorphic following chronic stress, suggesting divergent function of the GR system between male and female adolescent rats. Gene expression of Ppid, a positive regulator of the GR, was predicted by plasma estradiol and 34% lower in control adolescent females than males, indicating that sex steroids may play a role in the sexually dimorphic response. After chronic adolescent stress, females displayed elevated hippocampal expression of Bag1 and Ppid genes that was not observed in males. Overall, the GR output to an acute stressor, illustrated by transcription of Nr3c1 (encoding the GR), Bag1, Fkbp5, Ppid, and Src1, was significantly upregulated and differed in a sex-specific and chronic stress-dependent manner. This study provides new evidence for sex differences during development and adaptation of the glucocorticoid receptor chaperone system. [ABSTRACT FROM AUTHOR]

Abstract: Social interactions in rodents are rewarding and motivating and social isolation is aversive. Accumulating evidence suggests that disruption of the social environment in adolescence has long-term effects on social interactions, on anxiety-like behavior and on stress reactivity. In previous work we showed that adolescent isolation produced increased reactivity to acute and to repeated stress in female rats, whereas lower corticosterone responses to acute stress and decreased anxiety-related behavior were noted in isolated males. These results indicate a sex specific impact on the effects of social stress in adolescence. However, little is known about whether social isolation impacts behaviors related to affect and whether it does so differently in male and female rats. The present study investigated the impact of adolescent social isolation from day 30–50 of age in male and female Sprague Dawley rats on behavior in the forced swim test at the end of adolescence and in adulthood and on behavior in the sucrose preference test in adulthood. Adult female rats that were isolated in adolescence exhibited increased climbing on the first and second day of the forced swim test and showed an increased preference for sucrose compared to adult females that were group-housed in adolescence. There were no effects in male rats. The results indicate that social isolation in adolescence produces a stable and active behavioral phenotype in adult female rats. [Copyright &y& Elsevier]

Abstract: Stressful experiences during adolescence can alter the trajectory of neural development and contribute to psychiatric disorders in adulthood. We previously demonstrated that adolescent male rats exposed to repeated social defeat stress show changes in mesocorticolimbic dopamine content both at baseline and in response to amphetamine when tested in adulthood. In the present study we examined whether markers of adult dopamine function are also compromised by adolescent experience of social defeat. Given that the dopamine transporter as well as dopamine D1 receptors act as regulators of psychostimulant action, are stress sensitive and undergo changes during adolescence, quantitative autoradiography was used to measure [3H]-GBR12935 binding to the dopamine transporter and [3H]-SCH23390 binding to dopamine D1 receptors, respectively. Our results indicate that social defeat during adolescence led to higher dopamine transporter binding in the infralimbic region of the medial prefrontal cortex and higher dopamine D1 receptor binding in the caudate putamen, while other brain regions analyzed were comparable to controls. Thus it appears that social defeat during adolescence causes specific changes to the adult dopamine system, which may contribute to behavioral alterations and increased drug seeking. [Copyright &y& Elsevier]

Abstract: Evidence suggests that women are more susceptible to stress-related disorders than men. Animal studies demonstrate a similar female sensitivity to stress and have been used to examine the underlying neurobiology of sex-specific effects of stress. Although our understanding of the sex-specific effects of chronic adolescent stress has grown in recent years, few studies have reported the effects of adolescent stress on depressive-like behavior. The purpose of this study was to determine if a chronic mixed modality stressor (consisting of isolation, restraint, and social defeat) during adolescence (PND 37–49) resulted in differential and sustained changes in depressive-like behavior in male and female Wistar rats. Female rats exposed to chronic adolescent stress displayed decreased sucrose consumption, hyperactivity in the elevated plus maze, decreased activity in the forced swim test, and a blunted corticosterone response to an acute forced swim stress compared to controls during both adolescence (PND 48–57) and adulthood (PND 96–104). Male rats exposed to chronic adolescent stress did not manifest significant behavioral changes at either the end of adolescence or in adulthood. These data support the proposition that adolescence may be a stress sensitive period for females and exposure to stress during adolescence results in behavioral effects that persist in females. Studies investigating the sex-specific effects of chronic adolescent stress may lead to a better understanding of the sexually dimorphic incidence of depressive and anxiety disorders in humans and ultimately improve prevention and treatment strategies. [Copyright &y& Elsevier]

Abstract: A number of variables have been shown to moderate the relationship between stress and psychological functioning. Physical activity (PA) has the potential to influence some of these variables but there is mixed evidence that PA can moderate the stress and mental well-being relationship among adolescents. The aim of this cross-sectional study was to investigate if leisure time physical activity moderates the relationship between stress and psychological functioning (depression, anxiety, self-esteem) among Norwegian adolescents 13-18 years old (n =1508). The data were analysed using analysis of variance (MANOVA and MANCOVA). In preliminary analyses, girls reported higher scores of depression and anxiety and boys scored higher on self-esteem. Interaction effects of gender by age were found on all outcome variables. Stress was positively associated with depression and anxiety, and negatively associated with self-esteem. Higher frequency of leisure time physical activity was weakly associated with lower levels of depression and anxiety, and higher levels of self-esteem. The primary analyses revealed no support for leisure time physical activity as a moderator of the association between stress and psychological functioning. The differences between the present study and similar ones were discussed. [Copyright &y& Elsevier]