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1. Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours

Author

Bujko Mateusz, Kober Paulina, Statkiewicz Małgorzata, Mikula Michal, Ligaj Marcin, Zwierzchowski Lech, Ostrowski Jerzy, and Siedlecki Janusz Aleksander

Subjects
Diseases of the digestive system. Gastroenterology, RC799-869
Abstract

Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.

Published
2015
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3. Single nucleotide polymorphisms in the bovine SLC2A12 and SLC5A1 glucose transporter genes – the effect on gene expression and milk traits of Holstein Friesian cows.

Author

Zwierzchowski, Lech, Ostrowska, Małgorzata, Żelazowska, Beata, and Bagnicka, Emilia

Subjects
*SINGLE nucleotide polymorphisms, *GLUCOSE transporters, *GENE expression, *COWS, *GENES, *DAIRY farm management, *BOVINE mastitis
Abstract

In this study, novel single nucleotide polymorphisms (SNPs) were found in the 5′-regulatory regions (promoters) of the bovine glucose transporter (GT) genes SLC2A12 and SLC5A1. These polymorphisms were shown to associate with certain milk production traits in HF cows, including milk yield, milk composition, and somatic cell count. It was shown that the SNP g.-671C > G (NC_037336.1: g.72224078C > G) in the SLC2A12 gene could be an effective marker of cattle production traits and that genotypes CC and CG are associated with the best productivity. The polymorphisms found in the SLC5A1 gene promoter also influenced milk production traits in HF cows, albeit to a lesser extent, and we propose that these polymorphisms could be useful as genetic markers for milk production traits in marker-assisted selection (MAS); however, this must be confirmed on larger populations of cattle. In addition, the presence of polymorphisms within promoter regions appears to affect the expression of GT genes in the cow mammary gland and modify transcription factor (TF) binding capacity. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Additional file 10: Table S1. of Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma

Author

Kosciuczuk, Ewa, Paweł Lisowski, Jarczak, Justyna, Majewska, Alicja, Rzewuska, Magdalena, Zwierzchowski, Lech, and Bagnicka, Emilia

Abstract

Gene clusters differing in expression between the CoPS-1/2 (coagulase-positive Staphyloccoci in 1st or 2nd lactation) and H (Healthy) groups in the parenchyma of the cow mammary gland. Table S2. Gene clusters differing in expression between the CoPS-3/4 (coagulase-positive Staphyloccoci in 3rd or 4th lactation) and H (Healthy) groups in the parenchyma of the cow mammary gland. Table S3. Gene clusters differing in expression between the CoNS-1/2 (coagulase-negative Staphyloccoci in 1st or 2nd lactation) and H (Healthy) groups in the parenchyma of the cow mammary gland. Table S4. Gene clusters differing in expression between the CoNS-3/4 (coagulase-positive Staphyloccoci in 3rd or 4th lactation) and H (Healthy) groups in the parenchyma of the cowmammary gland. (DOCX 65Â kb)

Published
2017
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25. Additional file 3: Figure S1. of Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma

Author

Kosciuczuk, Ewa, Paweł Lisowski, Jarczak, Justyna, Majewska, Alicja, Rzewuska, Magdalena, Zwierzchowski, Lech, and Bagnicka, Emilia

Abstract

Figure S1. Significantly enriched Gene Ontology (GO) categories of genes differentially expressed in mammary secretory tissue between CoPS-1/2 and H cows. Figure S2. Gene network graphical representation. Cellular movement, hematological system development and function, and immune cell trafficking in secretory tissue between CoPS-1/2 and H cows. Figure S3. Significantly enriched Gene Ontology (GO) categories of genes differentially expressed in mammary secretory tissue between CoPS-3/4 and H cows. Figure S4. Gene network graphical representation: (A) Metabolic Disease, (B) Cellular Assembly and Organization, (C) Inflammatory Response, (D) Infectious Disease, Tissue Development in secretory tissue between CoPS-3/4 and H cows. Figure S5. Significantly enriched Gene Ontology (GO) categories of genes differentially expressed in mammary secretory tissue between CoNS-1/2 and H cows. Figure S6. Gene network graphical representation. Cell-To-Cell Signaling and Interaction in mammary secretory tissue between CoNS-1/2 and H cows. Figure S7. Significantly enriched Gene Ontology (GO) categories of genes differentially expressed in mammary secretory tissue between CoNS- 3/4 and H cows. Figure S8. Gene network graphical representation: (A) Cell Morphology, (B) Cell Assembly and Organization, (C) Cellular Growth and Proliferation, (D) Organismal Injury and Abnormalities, (E) Humoral Immune Response - in secretory tissue between CoNS-3/4 and H cows.(DOCX 1683 kb)

Published
2017
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29. Maternal placenta modulates a deleterious fetal mutation†

Author

Xu, Hongen, primary, Pausch, Hubert, additional, Venhoranta, Heli, additional, Rutkowska, Karolina, additional, Wurmser, Christine, additional, Rieblinger, Beate, additional, Flisikowska, Tatiana, additional, Frishman, Dmitrij, additional, Zwierzchowski, Lech, additional, Fries, Ruedi, additional, Andersson, Magnus, additional, Kind, Alexander, additional, Schnieke, Angelika, additional, and Flisikowski, Krzysztof, additional

Published
2017
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31. Single Nucleotide Polymorphism Discovery in Bovine Pituitary Gland Using RNA-Seq Technology

Author

Pareek, Chandra Shekhar, primary, Smoczyński, Rafał, additional, Kadarmideen, Haja N., additional, Dziuba, Piotr, additional, Błaszczyk, Paweł, additional, Sikora, Marcin, additional, Walendzik, Paulina, additional, Grzybowski, Tomasz, additional, Pierzchała, Mariusz, additional, Horbańczuk, Jarosław, additional, Szostak, Agnieszka, additional, Ogluszka, Magdalena, additional, Zwierzchowski, Lech, additional, Czarnik, Urszula, additional, Fraser, Leyland, additional, Sobiech, Przemysław, additional, Wąsowicz, Krzysztof, additional, Gelfand, Brian, additional, Feng, Yaping, additional, and Kumar, Dibyendu, additional

Published
2016
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38. Defensins: Natural component of human innate immunity

Author

Jarczak, Justyna, primary, Kościuczuk, Ewa M., additional, Lisowski, Paweł, additional, Strzałkowska, Nina, additional, Jóźwik, Artur, additional, Horbańczuk, Jarosław, additional, Krzyżewski, Józef, additional, Zwierzchowski, Lech, additional, and Bagnicka, Emilia, additional

Published
2013
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49. Glucose transporters in cattle - a review.

Author

Ostrowska, Małgorzata, Jarczak, Justyna, and Zwierzchowski, Lech

Subjects
GLUCOSE transporters, ANIMAL cell biotechnology, BODY fluids, LIPID synthesis, PROTEIN synthesis, GENETIC polymorphisms, CATTLE physiology
Abstract

Glucose is the major energy source for the animal cells. It is important substrate for protein and lipid synthesis. This sugar is absorbed into the cells from body fluids via glucose transporters -- structurally related trans-membrane proteins. There are two types of glucose transport - passive and active. The facilitative-glucose-transporter family of proteins (solute co-transporters GLUT, encoded by SLC2A genes) participates in energy-independent process of glucose transport. The Na+/ glucose co-transporter family proteins SGLT (solute carriers, encoded by SLC5A genes) mediate the Na+-linked transport of glucose against the electrochemical gradient. In this review, we describe genomic structure and function of the bovine glucose transporters. Intra-species comparative analyses o the amino acid identities of glucose transporter proteins is also described, as well as the information on the nucleotide sequence polymorphisms in the bovine glucose transporter genes. [ABSTRACT FROM AUTHOR]

Published
2015

50. Expression patterns of β-defensin and cathelicidin genes in parenchyma of bovine mammary gland infected with coagulase-positive or coagulasenegative Staphylococci.

Author

Kosciuczuk, Ewa M., Lisowski, Pawel, Jarczak, Justyna, Krzyzewski, Jozef, Zwierzchowski, Lech, and Bagnicka, Emilia

Subjects
BOVINE mastitis, CATTLE diseases, GENOMES, MAMMARY glands, STAPHYLOCOCCUS
Abstract

Background Mastitis is still considered to be the most economically important infectious disease in dairy cattle breeding. The immune response in mammary gland tissues could help in developing support strategies to combat this disease. The role of neutrophils and macrophages in the innate response of mammary gland is well known. However, the immune response in mammary gland tissues, including levels of antimicrobial peptide transcripts, has not been well recognized. Moreover, most studies are conducted in vitro, on cell cultures, or on artificially infected animals, with analysis being done within a several dozen hours after infection The aim of the study was to examine the in vivo transcript levels of beta-defensin and cathelicidins genes in cow mammary gland secretory tissue (parenchyma) with the chronic, recurrent and incurable mammary gland inflammation induced by coagulase-positive or coagulase-negative Staphyloccoci vs. bacteria-free tissue. Results The mRNA of DEFB1, BNBD4, BNBD5, BNBD10 and LAP genes, but not of TAP gene, were detected in all investigated samples regardless of the animals' age and microbiological status of the mammary gland, but at different levels. The expression of most of the betadefensin genes was shown to be much higher in tissues derived from udders infected with bacteria (CoPS or CoNS) than from bacteria-free udders, regardless of parity. Cathelicidins (CATH4, CATH5 and CATH6) showed expression patterns contrasting those of β-defensins, with the highest expression in tissues derived from bacteria-free udders. Conclusion Increased expression of genes encoding β-defensins in the infected udder confirms their crucial role in the defense of the cow mammary gland against mastitis. On the other hand, the elevated cathelicidin transcripts in non-infected tissues indicate their role in the maintenance of healthy mammary tissues. The expression levels of investigated genes are likely to depend on the duration of the infection and type of bacteria. [ABSTRACT FROM AUTHOR]

Published
2014
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