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754 results on '"Zuppi, Cecilia"'

28. Bile acids cause secretory phospholipase A2 activity enhancement, revertible by exogenous surfactant administration

Author

Luca, Daniele, Minucci, Angelo, Zecca, Enrico, Piastra, Marco, Pietrini, Domenico, Carnielli, Virgilio P., Zuppi, Cecilia, Tridente, Ascanio, Conti, Giorgio, and Capoluongo, Ettore D.

Subjects
Aspiration and aspirators -- Research, Bile acids -- Physiological aspects, Bile acids -- Health aspects, Phospholipases -- Physiological aspects, Phospholipases -- Health aspects, Health care industry
Abstract

Byline: Daniele Luca (1), Angelo Minucci (2), Enrico Zecca (3), Marco Piastra (1), Domenico Pietrini (1), Virgilio P. Carnielli (4,5), Cecilia Zuppi (2), Ascanio Tridente (6), Giorgio Conti (1), Ettore D. Capoluongo (2) Keywords: Bile acids; Phospholipase A2; Surfactant; Lung injury; Neonate; Infant Abstract: Background and purposes Bile acids have been implicated in some forms of acute lung injury, including meconium aspiration and bile acid pneumonia in neonates, or aspiration related ARDS in adults. Secretory phospholipase A2 (sPLA2) is now known as a key enzyme in the lung injury pathways and is supposed to be responsible for surfactant dysfunction. Our aim was to investigate the interaction between bile acids and sPLA2 in an extracellular environment representing an in vitro model of aspiration. Methods In vitro study using broncho-alveolar lavage (BAL) of 23 neonates/infants ( Results High concentrations of BA (5 umol/l) significantly increased (P = 0.012) sPLA2 activity, leading to increased surfactant catabolism. This finding was not observed with lower BA concentration and this is consistent with available literature data and may indicate an anionic activation of the enzyme by bile acids. Increased activity was significantly reverted by the addition of exogenous surfactant (P = 0.004) which was able to reduce sPLA2 activity almost to the baseline level. Conclusions BA are likely to contribute to lung injury, causing surfactant inactivation through the increased sPLA2 activity. Other mechanisms cannot be excluded and require further studies to be clarified. Author Affiliation: (1) Pediatric Intensive Care Unit, Department of Anaesthesiology and Intensive Care, University Hospital 'A.Gemelli', Catholic University of the Sacred Heart, L.go A. Gemelli 8, 00168, Rome, Italy (2) Laboratory of Clinical Molecular Biology, Institute of Biochemistry and Clinical Biochemistry, University Hospital 'A.Gemelli', Catholic University of the Sacred Heart, Rome, Italy (3) Division of Neonatology, Department of Pediatrics, University Hospital 'A.Gemelli', Catholic University of the Sacred Heart, Rome, Italy (4) Division of Neonatology, Institute of Mother and Child Health 'G. Salesi', Polytechnical University of Marche, Ancona, Italy (5) Nutrition Unit, Institute of Child Health and Great Ormond Street Hospital, London, UK (6) Prince Charles Hospital, Merthyr, UK Article History: Registration Date: 02/10/2008 Received Date: 17/06/2008 Accepted Date: 21/09/2008 Online Date: 14/10/2008

Published
2009

32. Epithelial lining fluid free IGF-I-to-PAPP-A ratio is associated with bronchopulmonary dysplasia in preterm infants

Author

Capoluongo, Ettore, Ameglio, Franco, Lulli, Paola, Minucci, Angelo, Santonocito, Concetta, Concolino, Paola, Di Stasio, Enrico, Boccacci, Simona, Vendettuoli, Valentina, Giuratrabocchetta, Giuseppe, De Cunto, Angela, Tana, Milena, Romagnoli, Costantino, Zuppi, Cecilia, and Vento, Giovanni

Subjects
Insulin-like growth factor 1 -- Research, Bronchopulmonary dysplasia -- Physiological aspects, Bronchopulmonary dysplasia -- Research, Infants (Premature) -- Physiological aspects, Infants (Premature) -- Research, Biological sciences
Abstract

Preterm newborns developing retinopathy of prematurity (ROP) and bronchopulmonary dysplasia (BPD) show persistently low levels of insulin-like growth factor-I (IGF-I) in sera. They also present higher free IGF-I concentrations in epithelial lining fluids (ELFs) and lung tissues. Pregnancy-associated plasma protein-A (PAPP-A) is a metalloproteinase that dissociates three binding proteins from the active form of IGF-I, namely free IGF-I. The present study analyzes the ELF concentrations of free IGF-I, PAPP-A, and their ratios in preterm newborns developing or not BPD, defined as [O.sub.2] dependence at 36 wk postmenstrual age. Bronchoalveolar lavage fluids of 41 infants (34 without and 7 with BPD) were analyzed on the 2nd and 4th day after birth. Infants developing BPD showed increased ELF free IGF-I and decreased PAPP-A concentrations on both days 2 and 4 compared with newborns without BPD. A nonsignificant trend between these 2 days was observed for free IGF-I (increasing) and PAPP-A (decreasing). On the same days, the free IGF-I-to-PAPP-A ratio was always significantly higher in patients developing BPD. These differences were more significant than those of IGF-I or PAPP-A when individually evaluated. A multivariate analysis confirmed the significance for free IGF-I on day 4, whereas the ratio was confirmed on both days 2 and 4. The same ratio was significantly correlated with some indexes of disease severity, such as hours of oxygen administration, days of hospitalization, and ROP severity scores. Finally, the ratio between ELF free IGF-I and PAPP-A appears to be a useful marker for lung injury of premature newborns. insulin-like growth factor I; pregnancy-associated plasma protein-A

Published
2007

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