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202 results on '"Zuk, Patricia A."'

1. Human levator veli palatini muscle: a novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations

Author

Bueno, Daniela Franco, Kabayashi, Gerson Shigueru, Pinheiro, Carla Cristina Gomes, Tanikawa, Daniela Y. S., Raposo-Amaral, Cassio Eduardo, Rocha, Diogenes Laercio, Ferreira, José Ricardo Muniz, Shibuya, Yoichiro, Hokugo, Akishige, Jarrahy, Reza, ZuK, Patricia A., and Passos-Bueno, Maria Rita

Published
2020
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2. Osteoblast Interactions Within a Biomimetic Apatite Microenvironment

Author

Tsang, Eric J, Arakawa, Christopher K, Zuk, Patricia A, and Wu, Benjamin M

Subjects
Engineering, Biomedical Engineering, 3T3 Cells, Amino Acid Chloromethyl Ketones, Animals, Apatites, Apoptosis, Bioengineering, Biomimetic Materials, Blood Proteins, Calcium, Caspase Inhibitors, Caspases, Cattle, Cell Adhesion, Cell Survival, Coated Materials, Biocompatible, Mice, Microscopy, Electron, Scanning, Osteoblasts, Phosphates, Serum Albumin, Bovine, Biomimetic apatite, Osteoblast, Protein adsorption
Abstract

Numerous reports have shown that accelerated apatites can mediate osteoblastic differentiation in vitro and bone formation in vivo. However, how cells interact within the apatite microenvironment remains largely unclear, despite the vast literature available today. In response, this study evaluates the in vitro interactions of a well-characterized osteoblast cell line (MC3T3-E1) with the apatite microenvironment. Specifically, cell attachment, spreading, and viability were evaluated in the presence and absence of serum proteins. Proteins were found to be critical in the mediation of cell-apatite interactions, as adherence of MC3T3-E1 cells to apatite surfaces without protein coatings resulted in significant levels of cell death within 24 h in serum-free media. In the absence of protein-apatite interaction, cell viability could be "rescued" upon treatment of MC3T3-E1 cells with inhibitors to phosphate (PO(4) (3-)) transport, suggesting that PO(4) (3-) uptake may play a role in viability. In contrast, rescue was not observed upon treatment with calcium (Ca(2+)) channel inhibitors. Interestingly, a rapid "pull-down" of extracellular Ca(2+) and PO(4) (3-) ions onto the apatite surface could be measured upon the incubation of apatites with α-MEM, suggesting that cells may be subject to changing levels of Ca(2+) and PO(4) (3-) within their microenvironment. Therefore, the biomimetic apatite surface may significantly alter the microenvironment of adherent osteoblasts and, as such, be capable of affecting both cell survival and differentiation.

Published
2011

4. Additional file 1 of Human levator veli palatini muscle: a novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations

Author

Bueno, Daniela Franco, Kabayashi, Gerson Shigueru, Pinheiro, Carla Cristina Gomes, Tanikawa, Daniela Y. S., Raposo-Amaral, Cassio Eduardo, Diogenes Laercio Rocha, Ferreira, José Ricardo Muniz, Shibuya, Yoichiro, Akishige Hokugo, Jarrahy, Reza, ZuK, Patricia A., and Passos-Bueno, Maria Rita

Abstract

Additional file 1. Supplementary Materials.

Published
2020
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7. Human levator veli palatini muscle: A novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations

Author

Bueno, Daniela Franco, primary, Kabayashi, Gerson Shigueru, additional, Pinheiro, Carla Cristina Gomes, additional, Tanikawa, Daniela Y S, additional, Raposo-Amaral, Cassio Eduardo, additional, Rocha, Diogenes Laercio, additional, Ferreira, José Ricardo Muniz, additional, Shibuya, Yoichiro, additional, Hokugo, Ashigue, additional, Jarrahy, Reza, additional, Zuk, Patricia A, additional, and Bueno, Maria Rita Passos, additional

Published
2020
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11. Contributors

Author

Adassi, Alaa, primary, Afrikanova, Iva, additional, Akashi, Koichi, additional, Anversa, Piero, additional, Atala, Anthony, additional, Avecilla, Scott T., additional, Baksh, Dolores, additional, Barrandon, Yann, additional, Bartelmez, Stephen H., additional, Bauer, Steven R., additional, Benhaim, Prosper, additional, Bianco, Paolo, additional, Blau, Helen M., additional, Bode, Hans, additional, Bonner-Weir, Susan, additional, Boulanger, Corinne A., additional, Brittan, Mairi, additional, Bronner-Fraser, Marianne, additional, Broxmeyer, Hal E., additional, Burt, Richard K., additional, Caldwell, Maeve A., additional, Camargo, Fernando D., additional, Caplan, Arnold I., additional, Carmeliet, Peter, additional, Cattaneo, Elena, additional, Chandran, Siddharthan, additional, Chang, Howard Y., additional, Chen, Xin, additional, Cheng, Tao, additional, Childs, Richard W., additional, Choi, Kyunghee, additional, Chowdhury, Dipanjan, additional, Chung, Yun Shin, additional, Collas, Philippe, additional, Crooks, Gay M., additional, Cossu, Giulio, additional, Davis, Brian R., additional, De Angelis, Gabriella Cusella, additional, Dent, Sharon Y.R., additional, Dick, John E., additional, Direkze, Natalie, additional, Dor, Yuval, additional, Driskell, Ryan R., additional, Dulac, Catherine, additional, Dunbar, Cynthia E., additional, Ema, Hideo, additional, Engelhardt, John F., additional, Enver, Tariq, additional, Evrard, Yvonne A., additional, Factor, Valentina M., additional, Finley, K. Rose, additional, Fletcher, Jennifer C., additional, Fuchs, Elaine, additional, Fuller, Margaret T., additional, Globerson, Amiela, additional, Goldberg, Victor M., additional, Goodell, Margaret A., additional, Göttgens, Berthold, additional, Gould, Elizabeth, additional, Green, Anthony Richard, additional, Grisham, Joe W., additional, Grompe, Markus, additional, Haylock, David N., additional, Hazlehurst, Anne L., additional, Hedrick, Marc H., additional, Horb, Marko E., additional, Huang, Jerry I., additional, Humes, H. David, additional, Ishii, Haruhiko, additional, Jin, David K., additional, Jones, D. Leanne, additional, Kajstura, Jan, additional, Kessinger, Anne, additional, Kintner, Chris, additional, Koyano-Nakagawa, Naoko, additional, Krumlauf, Robb, additional, Küntziger, Thomas, additional, LaBarge, Mark A., additional, Lansdorp, Peter M., additional, Lanza, Robert, additional, Lemischka, Ihor, additional, Leri, Annarosa, additional, Lucas, Joseph J., additional, Luttun, Aernout, additional, Lysaght, Michael J., additional, Madlambayan, Gerard J., additional, May, Gillian, additional, McKenzie, Joby L., additional, McKinney-Freeman, Shannon, additional, Melton, Douglas A., additional, Mirescu, Christian, additional, Moore, Malcolm A.S., additional, Morita, Yo-hei, additional, Nadal-Ginard, Bernardo, additional, Nakauchi, Hiromitsu, additional, Orlic, Donald, additional, Potten, Christopher S., additional, Preston, Sean, additional, Prockop, Darwin J., additional, Prokopishyn, Nicole L., additional, Rafii, Shahin, additional, Ramos, Carlos Almeida, additional, Raymond, Pamela A., additional, Robey, Pamela Gehron, additional, Rochat, Ariane, additional, Rodewald, Hans-Reimer, additional, Rosenthal, Nadia, additional, Rossi, Ferdinando, additional, Ruscetti, Francis W., additional, Sampaolesi, Maurilio, additional, Laurea, Maria Paola Santini, additional, Scadden, David T., additional, Seggewiss, Ruth, additional, Sen, Ranjan, additional, Sharp, J. Graham, additional, Shmelkov, Sergey V., additional, Siddiqui, Mohummad Minhaj, additional, Simmons, Paul J., additional, Slayton, William B., additional, Smith, Gilbert H., additional, Sommer, Lukas, additional, Spangrude, Gerald, additional, Srinivasan, Ramaprasad, additional, Szczypka, Mark S., additional, Takahashi, Yoshiyuki, additional, Tejada, Rafael, additional, Terada, Naohiro, additional, Thomas, E. Donnall, additional, Thomson, James A., additional, Thorgeirsson, Snorri S., additional, Tjwa, Marc, additional, Tosh, David, additional, Trainor, Paul A., additional, Traver, David, additional, Tumbar, Tudorita, additional, Vacanti, Joseph P., additional, Verda, Larissa, additional, Verfaillie, Catherine M., additional, Watt, Fiona M., additional, Weir, Gordon C., additional, Wilson, James W., additional, Wright, Nicholas A., additional, Yablonka-Reuveni, Zipora, additional, Yelick, Pamela C., additional, Yoo, Jung U., additional, Zandstra, Peter W., additional, Zakhary, Lisa, additional, Zhang, Wen Jie, additional, Zon, Leonard I., additional, and Zuk, Patricia A., additional

Published
2004
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19. Abstract P36

Author

Bakshi, Reena, primary, Hokugo, Akishige, additional, Segovia, Luis, additional, Zhou, Situo, additional, Rezzadeh, Kameron, additional, Zuk, Patricia, additional, and Jarrahy, Reza, additional

Published
2017
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21. MAPK signaling has stage-dependent osteogenic effects on human adipose-derived stem cells <italic>in vitro</italic>.

Author

Tsang, Eric J., Wu, Benjamin, and Zuk, Patricia

Subjects
ADIPOSE tissues, STEM cells, PROTEIN kinases, JNK mitogen-activated protein kinases, BONE growth
Abstract

Overview: The use of pro-osteogenic growth factors, such as BMP2, in human adipose-derived stem cell (ASC) osteogenesis is well described. Because these growth factors work via signal transduction pathways, such as the mitogen-activated protein kinase (MAPK) cascade, a study of the relationship between MAPK signaling and ASC osteogenesis was conducted. Materials and Methods: ERK, JNK, and p38MAPK activation were measured in ASCs osteo-induced using either dexamethasone or vitamin D3 and correlated with mineralization. Activation and mineralization were also measured without dexamethasone or using the glucocorticoid, cortisone. The expression of the MAPK phosphatase, MKP1, and its relationship to mineralization was also assessed. The effect of decreasing MAPK activation on mineralization through the use of exogenous inhibitors was examined along with siRNA-knockdown and adenoviral overexpression of ERK1/2. Finally, the effect of ERK1/2 overexpression on ASCs induced on PLGA scaffolds was assessed. Results: ASC mineralization in dexamethasone or vitamin D3-induced ASCs correlated with both increased ERK1/2 and JNK1/2 activation. ASCs induced without dexamethasone also mineralized, with JNK1/2 signaling possibly mediating this event. No link between cortisone induction and MAPK signaling could be ascertained. ASCs treated with ERK, JNK, or p38MAPK inhibitors showed decreased osteogenic gene expression and diminished mineralization. Mineralization levels were also affected by viruses designed to inhibit or augment ERK1/2 expression and activity. Finally, ASC mineralization appeared to be a balance between the MAPK kinase activity and MKP1. Conclusions: It is likely that MAPK signaling plays a significant role in ASC osteogenesis, affecting differentiation in kinase- and stage-specific manners. [ABSTRACT FROM AUTHOR]

Published
2018
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24. Abstract 133

Author

Rezzadeh, Kameron S., primary, Hokugo, Akishige, additional, Jewett, Anahid, additional, Kozlowska, Anna, additional, Segovia, Andres, additional, Zuk, Patricia, additional, Zhang, Zheyu, additional, and Jarrahy, Reza, additional

Published
2015
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25. Cell Interactions within Biomimetic Apatite Microenvironments

Author

Tsang, Eric, Wu, Benjamin M1, Zuk, Patricia A, Tsang, Eric, Tsang, Eric, Wu, Benjamin M1, Zuk, Patricia A, and Tsang, Eric

Abstract

Bioactive ceramics, such as calcium phosphate-based materials, have been studied extensively for the regeneration of bone tissue. Accelerated apatite coatings prepared from biomimetic methods is one approach that has had a history of success in both in vitro and in vivo studies for bone regeneration [1]-[4]. However, how cells interact within the apatite microenvironment remains largely unclear, despite the vast literature available today. In response, this thesis evaluates the in vitro interactions of a well-characterized osteoblast cell line with the apatite microenvironment. For this, the cellular response to several aspects of the apatite microenvironment was separately examined in order to piece together a more simplified picture of a complex and dynamic system: (1) the influence of accelerated apatite on local calcium and phosphate concentration, (2) the role of protein adsorption onto apatite surfaces, and (3) apatite surface charge. Furthermore, the immunopotentiating properties of apatite were also characterized by examining monocyte response to 2D and 3D apatite-coated model culture systems in vitro. A rapid "pull-down" of extracellular Ca2+and PO43- ions onto the apatite surface could be measured upon the incubation of apatites in cell culture medium, suggesting that cells may be subject to changing levels of Ca2+and PO43- within their microenvironment. Changing levels of Ca2+and PO43- are likely to have large implications for the biological response to apatites as increasing concentrations above a certain threshold were confirmed to be cytotoxic. Proteins were found to be critical in the mediation of cell-apatite interactions, as adherence of MC3T3-E1 cells to apatite surfaces without protein coatings resulted in significant levels of cell death within 24 hours in serum-free media. In the absence of protein-apatite interaction, cell viability could be restored upon treatment of the cells with inhibitors to PO43- transport, suggesting that PO43- uptake ma

Published
2014

31. Abstract 206

Author

Zuk, Patricia, primary, Li, A, additional, Buck, A, additional, Hokugo, A, additional, Sorice, S, additional, Lee, J, additional, and Jarrahy, R, additional

Published
2013
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49. The Role of Cytoprotective Cytokines in Cardiac Ischemia/Reperfusion Injury

Author

Anderson, Corey D., primary, Heydarkhan-Hagvall, Sepideh, additional, Schenke-Layland, Katja, additional, Yang, Jin Quan, additional, Jordan, Maria C., additional, Kim, Jeanne K., additional, Brown, David A., additional, Zuk, Patricia A., additional, Laks, Hillel, additional, Roos, Kenneth P., additional, MacLellan, W. Robb, additional, and Beygui, Ramin E., additional

Published
2008
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