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Purpose: Physalis angulata Linn. (Ciplukan) is a plant widely used in traditional medicine in subtropical and tropical regions. Most studies focus on its antioxidant and anti-inflammatory activity. Many studies also reported its therapeutic potential for treating cancer, malaria, hepatitis, rheumatism, liver problems, and tumors, but few studies have reported its anti-fibrosis activity. Here, we aimed to investigate the potential of P. angulata as an antioxidant and anti-inflammatory that may be correlated with its anti-fibrosis action. Methods: In our study, we treated 3T3-L1 and TGF-β-induced 3T3-L1 cells with an ethanol extract of P. angulata. We then monitored the cell's response, evaluated the antioxidant activity using an MTT assay, and observed the cells' migration using the cell scratch assay. We used RT-PCR to determine the expression of HIF-1α and IL-6 on TGF-β-induced 3T3-L1 cells. Results: The ethanol extract of P. angulata showed antioxidant activity and promoted cell proliferation on 3T3-L1 cells. Interestingly, the extract inhibited the migration of TGF-β-induced 3T3-L1 cells. Further analysis revealed that the extract could inhibit HIF-1α expression and suppress IL-6 expression on TGF-β-induced 3T3-L1 cells. Conclusion: The ethanol extract of P. angulata showed antioxidant and anti-inflammation activities in 3T3-L1 cells. Both activities are associated with the antifibrotic activity of P. angulata's ethanol extract. [ABSTRACT FROM AUTHOR]

Cancer is a complicated and ever-evolving disease that remains a significant global cause of disease and mortality. Its complexity, which is evident at the genetic and phenotypic levels, contributes to its diversity and resistance to treatment. Numerous scientific investigations on human and animal models demonstrate the potential of phytochemicals in cancer prevention. Coffee has been shown to possess potent anti-carcinogenic properties, and studies have documented the consumption of coffee as a beverage reduces the risk of cancer occurrence. The major secondary metabolites of coffee, named caffeine and chlorogenic acid, have been linked to anti-inflammatory and antineoplastic effects through various signaling. In light of this, this review article provides a comprehensive analysis based on studies in anticancer effects of coffee, chlorogenic acid, and caffeine published between 2010 and 2023, sourced from Scopus, Pubmed, and Google Scholar databases. We summarize recent advances and scientific evidence on the association of phytochemicals found in coffee with a special emphasis on their biological activities against cancer and their molecular mechanism deemed potential to be used as a novel therapeutic target for cancer prevention and therapy. [ABSTRACT FROM AUTHOR]

Molecular imprinting technology is a promising technique for separating, purifying, and enriching target compounds with high selectivity and sensitivity. The molecularly imprinted polymer (MIP) technique has developed rapidly in the separation and purification of chemical compounds and secondary metabolites of natural products. However, there are real challenges to the isolation of target compounds in natural products such as the unavailability of target compounds and high cost, so it is necessary to develop another approach to MIP synthesis. The dummy template is a solution to the unavailability of target compounds as it will produce a binding site that is specific enough for the target compound through covalent or non‐covalent bonds and the presence of the same functional groups between the target compound and the dummy will result in an interaction between the target compound and the binding site made using the dummy template. In addition, this dummy MIP (DMIP) is the best solution for overcoming the template leakage in traditional MIPs so that the DMIP technique is very good for removing and purifying compounds that are allergenic or toxic in natural product samples (high traceability). This review article will discuss the development of DMIPs in the separation and purification of compounds in natural products and the factors that affect their performance. Our discussion highlights the importance of selecting the right dummy template for optimal DMIP performance. This selection should be based on factors such as functional group similarity, being in the same bioactive compound group, and structural skeleton similarity. In addition, the selection and composition of other DMIP components can also affect the analytical performance of the DMIP. [ABSTRACT FROM AUTHOR]

Breast cancer is the highest number of cancer cases in the world with 7.8 million cases over the last 5 years. The number of cancer cases in Indonesia has also increased from 1.4 per mile (2013) to 1.49 per mile (2018). In treating cancer, people in Indonesia choose surgery (61.8%), chemotherapy (24.9%), radiotherapy (17.3%), and other treatments including traditional health services (Yankestrad) (24.1%). However, all of these options have many side effects and a small percentage of cure, especially if the cancer is detected late. Therefore, a Community Service Activity was held integrated with the Students Workshop Program (PPM-KKNM Integratif) to conduct education of early detection methods for breast cancer, SADARI, and the introduction of yellow Cempaka (Michelia champaca L.) as a natural anti-breast cancer. PPM-KKNM Integratif program activities have been carried out in a hybrid meeting in Babakan Ciwaringin Village, Cirebon. SADARI topic that was delivered through audio-visual media has been proven to increase the knowledge of participants, as indicated by an increase in the average score from 62.28 points in the pre-test to 70.37 points in the post-test. After being given material topics about the Cempaka Kuning and its properties as a candidate for a natural anti-breast cancer drug, the participants' knowledge also increased by 13.54 points from the average pre-test score, so that around 70% of participants got ≥ 80 points. It can be concluded that the aim of the activity was achieved, that the people of Babakan Village, represented by PKK cadres and village officials, have been educated about early detection of breast cancer (SADARI) as well as the efficacy and potential of yellow cempaka bark. After completing the activity, it is hoped that the community will care more about the health of themselves and their families, especially to prevent breast cancer disease. [ABSTRACT FROM AUTHOR]

Copyright of Indonesian Journal of Gastroenterology, Hepatology & Digestive Endoscopy is the property of Indonesian Society of Gastroenterology/Assoc for the Study of the Liver/Soc. for Digestive Endoscopy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

An increasing proportion of the global population have or are at risk of developing non-alcoholic fatty liver disease (NAFLD). Fibrosis is the most important predictor of prognosis in NAFLD patients. Ciplukan (Physalis angulata Linn.) has been reported to have antifibrotic potency in CCl4-induced liver fibrotic rats, so this study was designed to evaluate the antifibrotic effect of ciplukan extract on liver function, inflammation, and cholesterol levels. The liver fibrosis model was established using 20% margarine injected subcutaneously 8 times twice a week for 4 weeks in 35 male and 35 female Wistar rats. The rats were then treated orally with ciplukan extract (CPL) in 70% alcohol, starting the 6th week of treatment with 2 different doses, namely 13.5 mg (CPL-1) and 27 mg (CPL-2) every day for 4 weeks. The histopathological changes in the liver were analyzed by Haematoxylin Eosin (HE) staining. Serum IL-6 and TGF-ß1 levels were determined by ELISA. ALT and cholesterol levels were measured using a diagnostic kit. Single and multiple doses of ciplukan extract with or without standard therapy (Vitamin E) reduced the fibrotic scores to 1.30±0.95 (p=0.001), TGF-ß1 levels to 24.20±2.02 ng/mL (p = 0.000), IL-6 levels to 1.68±0.52 pg/mL (p=0.156), ALT levels to 104.57±2.02 U/mL (p=0.001), and cholesterol levels to 81, 07±2.02 mg/dL (p=0.000). In conclusion, the ciplukan herb ethanol extract possesses potent liver antifibrotic activity, thus it is a potential new liver antifibrotic drug. [ABSTRACT FROM AUTHOR]

The study was purposed to investigate the antibacterial effect of red roselle (Hibiscus sabdariffa L.) calyx decoction on Staphylococcus sp. growth inhibition that could be useful to overcome staphylococcal infection. Materials and Methods: The calyxes of the roselle were extracted using the decoction method by boiling fresh red roselle calyxes until the temperature reached 90°C for 30 min. The antibacterial effect of the decoct was tested against Staphylococcus aureus ATCC 11784, Staphylococcus epidermidis ATCC 13228, Staphylococcus warneri ATCC 3340, and Staphylococcus xylosus ATCC 3342 using the agar diffusion method. The minimum inhibitory concentration value (MIC) was done using the agar macrodilution method. The antibacterial of the decoct then compared to tetracycline Hydrochloride (HCl). Results: The roselle decoct possessed the antibacterial activity with broad spectrum against all tests Staphylococcus in dilutions ranging from 0.41 to 0.81% w/v, except the MIC against S. aureus 11,748, reached the highest MIC value at 0.81–1.62% w/v. The anti-staphylococcal comparison values of roselle decoct against tetracycline HCl were S. aureus (1:5.67), S. epidermidis (1:1.10), S. warneri (1:1.24), and S. xylosus (1:5.04). Conclusion: This study indicated clear evidence supporting the red roselle calyx in the decoct preparation use in treating staphylococcal infection-related to bacteria. [ABSTRACT FROM AUTHOR]

The leaves of Garcinia celebica strongly inhibit the proliferation of MCF-7 human breast adenocarcinoma cell lines. The present study focused on investigating the active anticancer and antiproliferative compound from the G. celebica leaves and assessing its mechanism of action. Ethanol extracts of G. celebica were fractionated based on their polarity using n -hexane, ethyl acetate and water. The antiproliferative properties were tested in vitro against MCF-7 human breast cancer cell lines using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. The active compound was subsequently isolated using column chromatography and identified by nuclear magnetic resonance. The characterized compound was also tested for its antiproliferative properties and the mechanism by which it induces apoptosis in MCF-7 cells by western blot analysis of the activated apoptotic proteins. This resulted in the isolation of a friedolanostane triterpenoid, which was determined to be methyl-3α, 23-dihydroxy-17,14-friedolanstan-8,14,24-trien-26-oat. This compound inhibited MCF-7 cell proliferation in a time- and dose-dependent manner with IC 50 values of 82 and 70 µM for the 24 and 48 h treatments, respectively. Furthermore, the western blot analysis suggested that the compound exerted its anticancer activities by promoting apoptosis through the inhibition of the oncogenic protein Akt, thereby increasing the expression of poly (ADP-ribose) polymerase (PARP) protein. These results suggest that methyl-3α,23-dihydroxy-17,14-friedolanstan-8,14,24-trien-26-oat is the anticancer compound found in G. celebica, providing a basis for its potential use in cancer disease management.