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Li-rich Mn-based layered oxides are proposed to be candidates for high-energy Li-ion batteries. However, their large-scale production is still hampered by poor rate capability and severe voltage decay. It was mainly attributed to the irreversible oxygen loss, which induces transition metal ion migration, electrolyte consumption, and structural evolution. Herein, we propose an effective strategy of phosphorylation, in which the phosphate ion is induced to remove the surface labile oxygen. It urges the Li

This paper discussed 3 innovative comparator topologies proposed in last several years. Depends on the target applications, each of the 3 designs achieved significant performance gains. Compared with conventional design, the comparator with self-calibrating technique has low offset voltage, small die area, low noise, and low power consumption. The comparator with regeneration operating midway realizes dramatically low operation delay with acceptable power consumption. The comparator with latch structure on amplifier stage implements circuit with high speed under low supplied voltage.

Hepatocyte growth factor receptor (HGFR or Met) upregulation has been proven to play important roles in non-small cell lung carcinoma (NSCLC). Interestingly, chemoresistance against epidermal growth factor receptor (EGFR) inhibitors including erlotinib and gefitinib was also related to Met. Targeting bromodomain and extra terminal domain (BET) proteins, especially BRD4, has shown inhibitory effects on lung cancer, but the mechanism is unclear. Herein, we found that JQ1 (BET inhibitor) suppressed NSCLC cell growth, reduced the Met expression, and contributed to inactivation of PI3K/Akt and MAPK/ERK pathways. Moreover, another BET protein inhibitor I-BET151, or BRD4 depletion, also inhibited NSCLC cell growth and downregulated Met. JQ1 inhibited HGF-induced cell growth and Met/PI3K/Akt activation, also inhibited A549 tumor growth in xenograft mouse models, in parallel with Met downregulation. Moreover, JQ1 inhibited the growth of paired erlotinib-sensitive and resistant HCC827 cells in parallel with Met downregulation and PI3K/Akt signaling inactivation. JQ1 also exerted inhibitory influences on the growth of erlotinib-sensitive and resistant HCC827 tumors in xenograft mouse models. These results suggested that targeting BET proteins inhibited NSCLC via downregulating Met and inactivating PI3K/AKT pathway. Our findings reveal a novel mechanism of BET proteins implicated in NSCLC progression with Met taken into consideration.

Supplementary Figure S2 shows SUR1 regulated the growth of NSCLCs, and the expression levels of phosphorylated p70S6K and KLF4.

Supplementary Table S1 shows the clinicopathological characteristics of non-small cell lung carcinoma patients in Fig. 1C.

Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive potassium channels (KATP channels) and the receptor of antidiabetic drugs, such as glibenclamide, which induce insulin secretion in pancreatic β cells. However, the expression and role of SUR1 in cancer are unknown. In this study, we found that SUR1 expression was elevated in human non–small cell lung carcinoma (NSCLC) tissues and cell lines. SUR1 silencing suppressed the growth of NSCLC cells, while SUR1 overexpression promoted cell growth. Targeting SUR1 with glibenclamide suppressed cell growth, cell-cycle progression, epithelial–mesenchymal transition (EMT), and cell migration. Moreover, SUR1 directly interacted with p70S6K and upregulated p70S6K phosphorylation and activity. In addition, glibenclamide inhibited p70S6K, and overexpression of p70S6K partially reversed the growth-inhibiting effect of glibenclamide. Furthermore, glibenclamide upregulated the expression of the tumor suppressor Krüppel-like factor 4 (KLF4), and silencing KLF4 partially reversed the inhibitory effect of glibenclamide on cell growth, EMT, and migration. We found that SUR1 targeted p70S6K to downregulate KLF4 expression by enhancing DNA-methyltransferase 1–mediated methylation of the KLF4 promoter. Finally, in xenograft mouse models, SUR1 expression silencing or glibenclamide treatment inhibited the growth of A549 tumors, downregulated p70S6K activity, and upregulated KLF4 expression. These findings suggested that SUR1 expression was elevated in some NSCLC tissues and functioned as a tumor enhancer. Targeting SUR1 with glibenclamide inhibited NSCLC through downregulation of p70S6K activity and subsequent upregulation of the expression of the tumor suppressor gene KLF4. SUR1 can be developed as a new target for cancer therapy and glibenclamide has potential anticancer effects.

By modulating the d band center via dual metal doping, and thus optimizing the adsorption free energy change for intermediates, the as prepared Ni–Mn–FeP exhibits outstanding catalytic performance toward both the OER (η10 = 185 mV) and HER (η10 = 103 mV).

Herein, a strand displacement amplification (SDA)-assisted CRISPR/Cas12a (LbCpf1) electrochemiluminescence (ECL) biosensor was fabricated for ultrasensitive identification of

Polyunsaturated fatty acid (PUFA) and eicosapentaenoic acid (EPA) are essential for the health of aquatic organisms and human beings. In order to optimize the culture conditions of Chaetoceros lorenzianus, effects of different iron sources (FeC6H5O7, FeCl3, FeSO4) and different iron concentrations (0.10 mg L− 1, 0.25 mg L− 1, 0.50 mg L− 1, 0.75 mg L− 1, 1.00 mg L− 1, 1.25mg L− 1) on C. lorenzianus were studied. The results showed that different iron sources and different iron concentrations had significant effects on the biomass, chlorophyll A, total lipid and fatty acids of C. lorenzianus (P 4 and FeC6H5O7, FeCl3 had better effects on the growth, chlorophyll a, total lipids, and n-3 PUFA (EPA and DHA) content of C. lorenzianus. The optimum concentration of FeCl3 for the growth and synthesis of chlorophyll a in C. lorenzianus was 0.75–1 mg L− 1, and the optimum concentration for lipid accumulation was 0.25 mg L− 1. The secondary culture method can be used in large-scale culture, that is, the initial culture is performed under the optimal iron concentration (0.75mg L− 1 FeCl3) for obtaining higher biomass, and then, the subsequent culture is performed under a low iron concentration(0.25 mg L− 1 FeCl3) to accumulate higher total lipids.

The creation of three-dimensional models from an unorganized set of points is an active research area in computer graphics. One of the purposes of this study is to explore the 3D reconstruction of a cube-type artificial reef (CTAR) set by linear structured light and binocular stereo vision technology in an underwater environment. The experimental setup is composed of two cameras in a stereo vision configuration. The alpha shapes method can be used to construct a surface that most closely reflects the artificial reef set described by the points. A parameter study is conducted to assess the scales of the set (i.e., usable volume, surface area, projected area, height, and base diameter) on the basis of 3D reconstruction. Experimental results show that the quality of 3D reconstruction in an underwater environment is acceptable for estimating the scale size of the CTAR set. According to the measurement of the scale sizing of the CTAR set, the relationships between the parameters of the CTAR set and the number of CTAR modules were determined. Moreover, the usable volume of the CTAR set can be estimated depending on the basis of the number of CTAR modules.

Ni-rich layered oxides are promising cathodes to satisfy the long driving range of electric vehicles. However, Li/Ni mixing becomes a critical issue, which affects nearly all of the aspects of elec...

This work proposed a novel double-engine powered paper photoelectrochemical (PEC) biosensor based on an anode-cathode cooperative amplification strategy and various signal enhancement mechanisms, which realized the monitoring of multiple miRNAs (such as miRNA-141 and miRNA-21). Specifically, C

Low initial Coulombic efficiency (ICE) is an obstacle for practical application of Li-rich Mn-based layered oxides (LLOs), which is closely related with the irreversible oxygen evolution owing to the overoxidized reaction of surface labile oxygen. Here we report a NH4 F-assisted surface multicomponent integration technology to accurately control the ICE, by which oxygen vacancies, spinel-layered coherent structure, and F-doping are skillfully integrated on the surface of treated LLOs microspheres. Though the regulation on the removed amount of labile oxygen by surface integrated structure, the ICE of LLOs cathodes can adjust from starting value to 100 %. X-ray absorption spectroscopy, refined X-ray diffraction, and scanning transmission electron microscopy show that the removed labile oxygen mainly comes from Li2 MnO3 -like structure. Even operating at a high cut-off voltage of 5 V, the capacity retention of integrated sample at 200 mA g-1 is still larger than 98 % after 100 cycles.

We reported an economic and practical ball-milling method for the synthesis of tetrahydroquinoline derivatives via a ‘one-pot’ three-component Diels-Alder reaction of anilines, aldehydes and alkenes catalyzed by phosphotungstic acid at room temperature. For this reaction, a simple “one-pot” ball-milling operation was conducted, readily available starting materials were employed, ‘one-pot’ conditions were applied, and the most important was to use inexpensive and environmentally friendly catalyst phosphotungstic acid. Various tetrahydroquinolines, which might be potentially applicable in the pharmaceutical and biochemical areas, were conveniently synthesized in moderate to excellent yields.

The global demand for bioenergy and nanomaterial utilization has significantly contributed to their source depletion. The impact of outdoor pollutant emissions on residents' health, behavior, physical activity, and sleeping behavior is much less explored. Producing biofuels from organic waste has the potential to unite budget-friendliness and long-term sustainability. Primary biofuels are produced specifically to mitigate environmental problems, and secondary biofuels are produced specifically to mitigate other environmental problems. The high level of pollutants ina certain area can severely restrict human activities and ultimately influence their whole life. The study aims to empirically evaluate the influence of nanomaterials pollutants emissions influencing outdoor recreation in China because its economy is based on fast-paced industrialization and urbanization. Various datasets were collected, including domestic and international tourists from the annual statistics for 2011-2020 from the National Bureau of Statistics of China. Ten years of annual METAR data of visibility (VR), temperature (T), relative humidity (Demirhan), and wind speed (WS) from 30 metrological stations at international airports. Annual average AOD (Aerosol's Optical Depth), O

Herein, a high-sensitivity photoelectrochemical (PEC) biosensor was developed based on CdS quantum dots (QDs) sensitized porous hexagonal boron nitride (h-BN) nanosheets (NSs) and the multiple sites tripodal DNA walker (TDW) formed by catalytic hairpin assembly (CHA). Noticeably, the porous structure of h-BN NSs gives it a lasting gift of large specific surface areas and extensive active reaction sites, which makes it possible to be employed as photoelectric substrate material. The h-BN/CdS QDs composite material promotes the transmission of photogenerated electrons and holes, the outstanding photoelectric conversion efficiency. Meanwhile, the CHA-formed TDWs triggered by miRNA-141 moved on track strand-functionalized electrode, so that a large number of alkaline phosphatase (ALP) was immobilized on the electrode surface and further in situ catalyzed ascorbic acid 2-phosphate (AAP) to produce ascorbic acid (AA) as the electron donor. As the result of the decisive influence of electron donor on PEC biosensor, the sensitive detection of miRNA-141 was realized. The proposed PEC biosensor displayed an excellent linear relationship ranging from 1 fM to 100 nM with a detection limit of 0.73 fM, providing a powerful strategy for early clinical diagnosis and biomedical research.

Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive potassium channels (KATP channels) and the receptor of antidiabetic drugs, such as glibenclamide, which induce insulin secretion in pancreatic β cells. However, the expression and role of SUR1 in cancer are unknown. In this study, we found that SUR1 expression was elevated in human non–small cell lung carcinoma (NSCLC) tissues and cell lines. SUR1 silencing suppressed the growth of NSCLC cells, while SUR1 overexpression promoted cell growth. Targeting SUR1 with glibenclamide suppressed cell growth, cell-cycle progression, epithelial–mesenchymal transition (EMT), and cell migration. Moreover, SUR1 directly interacted with p70S6K and upregulated p70S6K phosphorylation and activity. In addition, glibenclamide inhibited p70S6K, and overexpression of p70S6K partially reversed the growth-inhibiting effect of glibenclamide. Furthermore, glibenclamide upregulated the expression of the tumor suppressor Krüppel-like factor 4 (KLF4), and silencing KLF4 partially reversed the inhibitory effect of glibenclamide on cell growth, EMT, and migration. We found that SUR1 targeted p70S6K to downregulate KLF4 expression by enhancing DNA-methyltransferase 1–mediated methylation of the KLF4 promoter. Finally, in xenograft mouse models, SUR1 expression silencing or glibenclamide treatment inhibited the growth of A549 tumors, downregulated p70S6K activity, and upregulated KLF4 expression. These findings suggested that SUR1 expression was elevated in some NSCLC tissues and functioned as a tumor enhancer. Targeting SUR1 with glibenclamide inhibited NSCLC through downregulation of p70S6K activity and subsequent upregulation of the expression of the tumor suppressor gene KLF4. SUR1 can be developed as a new target for cancer therapy and glibenclamide has potential anticancer effects.

Background: AEG-1 has been proven to be tumor enhancer in gastric cancer. However, its mechanism has not yet been fully clarified. Methods: Gain-of-function and loss-of-function experiments were conducted to determine the role of eIF4E in AEG-1-induced growth of gastric cancer cells and xenografts of a nude mouse model. Western blot analysis and SRB assay were used to determine the protein expression levels and survival cell numbers. Results: Silencing the expression of AEG-1 inhibited the growth of gastric cancer cells in parallel with a decreased eIF4E and cyclin D1 expression; however, the overexpression of AEG-1 promoted cell growth and increased eIF4E and cyclin D1 expression. Moreover, the overexpression of eIF4E partially reversed the AEG-1 silencing-induced reduction of cyclin D1 and the inhibition of cell growth. An eIF4E knockdown also partially reversed the AEG-1 overexpression-induced upregulation of cyclin D1 and cell growth. Notably, manipulating the expression of eIF4E did not affect the expression of AEG-1. Finally, the silencing of AEG-1 expression inhibited the growth of SGC-7901 xenografts in parallel with the downregulation of eIF4E and cyclin D1 expression in the nude mouse model. Conclusion: AEG-1 promoted the growth of gastric cancer through upregulation of eIF4E/cyclin D1 signaling pathway.

Background Marine diatoms were considered as a promising alternative to microbial resources for the development of biofuels, food additives, medicines and chemical materials. Light of wavelength is a principal element that can easily be controlled in microalgal scale-culture and influence the growth, pigment composition, and lipids accumulation of algal cells. With the increasing implementation of light-emitting diodes (LEDs) in microalgal production systems (photobioreactors), a measure of light quality controlled by LED could be suitable to improve microalgal yields. Results In this study, Cylindrotheca Closterium, a widely used diatom, influenced by five light quality (white, red, blue, green and yellow light) was tested for its effects of growth rates, chlorophylls contents, total lipids contents and fatty acids compositions. The growth and chlorophyll experiments also showed that the green and yellow light significantly improve the growth rates and chlorophylls contents than other groups (P C. closterium (P P P C. closterium. White light increased total lipid yields, while blue light was superior in increasing the production of unsaturated fatty acids, especially on the timnodonic acid (EPA). The application of two-step methods to increase the production of biomass and fatty acids is an effective measure for the cultivation of C. closterium; green light is used to increase the growth, followed by white light cultivation to improve total lipids or blue light to enhance the proportion of PUFAs of C. closterium.

The bromodomain and extra‑terminal domain (BET) family proteins are essential epigenetic regulators in lung cancer. However, BET inhibitors have not had the anticipated therapeutic efficacy. Combined treatment using BET inhibitors along with other drugs had favorable therapeutic effects but the underlying molecular mechanisms remain elusive. The aim of the present study was to investigate the antineoplastic effects and mechanisms of a combination of a BET inhibitor and paclitaxel or cisplatin in non‑small cell lung cancer (NSCLC). By using the online Kaplan‑Meier plotter, it was revealed that increased mRNA levels of several BET protein‑coding genes were associated with poor prognosis in NSCLC. SRB assay results revealed that pharmaceutical or genetic targeting of BET proteins suppressed the growth of NSCLC cells. Inhibition of BET protein expression, in combination with the use of chemotherapeutic drugs such as paclitaxel and cisplatin, further restrained NSCLC cell growth in a synergistic manner. Mechanistically, this combination of suppression of BET expression and chemotherapeutic treatment blocked NSCLC cell growth by inhibiting autophagy and promoting apoptosis, which were revealed by both western blot and ELISA results. The present findings revealed a new rationale for using a combination of BET inhibitors with chemotherapy in NSCLC treatment.

Li-rich Layered Oxides (LLOs), considered as the most promising cathode material with high theoretical specific capacity (>250 mAh g−1) in Lithium-Ion Batteries (LIBs), still suffer from severe intrinsic weaknesses such as low initial coulombic efficiency, poor rate capability, limited cycle life and severe voltage decay. These drawbacks are believed to originate from Transition Metal (TM) ion migration, irreversible oxygen release and cathode-electrolyte interfacial reactions etc. Owing to similar cubic close-packed oxygen arrays with LLOs, spinel phase, which greatly facilitates the transport of Li-ion, protection from organic electrolyte, the suppression of structural collapse and pre-extraction combining with the accommodation of Li-ion at low voltage, is widely applied to ameliorate electrochemical performance. In this article, we systematically review recent progresses of spinel phase on LLOs and comprehensively summarize the effectiveness of proposed strategies in improving the electrochemical performance of LLOs, including surface coating, spinel-layered coherent component, in-situ spinel-layered integration and synergistic effects of spinel with other components. Finally, we discuss the main barrier to the commercialization of LLOs and give advice for future development of LLOs for high-energy–density LIBs.

Combustion is the main source of particulate matter with a diameter of 2.5 μm or less (PM2.5). Thus, effective and economical combustion control methods are necessary. In our previous research, we ...

To control PM2.5 from solid waste combustion, combustion simulation, thermogravimetric analysis, and scanning electron microscopy were performed. Remarkable PM2.5 formation is an explosive release accompanied by acute weight loss and heat release over an interval of 300–400 °C. Sawdust starts to emit PM2.5 from approximately 200 °C. Plastic shows a little lag in PM2.5 emission from approximately 350 °C and constantly emits PM2.5 until 600 °C. When the temperature was 55–130 °C above of the ignition temperature or 20−65 °C above of the maximum weight loss temperature, the highest PM2.5 concentration was detected. Among combusted single wastes, plastic emits the maximum amount of PM2.5 at 10.7 mg/g, followed by rags (6.59 mg/g), sawdust (5.70 mg/g), food waste (5.49 mg/g), and paper (2.86 mg/g). Diverse sources have varied morphologies, which would help in identifying PM2.5 from various wastes. PM2.5 emitted by plastic is a well-distributed particulate that is spherical in shape. PM2.5 emitted by rag and fo...

Background: AEG-1 has been proven to be tumor enhancer in gastric cancer. However, its mechanism has not yet been fully clarified. Methods: Gain-of-function and loss-of-function experiments were conducted to determine the role of eIF4E in AEG-1-induced growth of gastric cancer cells and xenografts of a nude mouse model. Western blot analysis and SRB assay were used to determine the protein expression levels and survival cell numbers. Results: Silencing the expression of AEG-1 inhibited the growth of gastric cancer cells in parallel with a decreased eIF4E and cyclin D1 expression; however, the overexpression of AEG-1 promoted cell growth and increased eIF4E and cyclin D1 expression. Moreover, the overexpression of eIF4E partially reversed the AEG-1 silencing-induced reduction of cyclin D1 and the inhibition of cell growth. An eIF4E knockdown also partially reversed the AEG-1 overexpression-induced upregulation of cyclin D1 and cell growth. Notably, manipulating the expression of eIF4E did not affect the expression of AEG-1. Finally, the silencing of AEG-1 expression inhibited the growth of SGC-7901 xenografts in parallel with the downregulation of eIF4E and cyclin D1 expression in the nude mouse model. Conclusion: AEG-1 promoted the growth of gastric cancer through upregulation of eIF4E/cyclin D1 signaling pathway.

In order to improve the hydrothermal deoxygenation of organic acids as well as to reduce the cost, we prepared a Cu-Ce/

For the sake of collecting deep seawater samples automatically and promoting unmanned marine environmental monitoring technology level, a deepwater sample collection for unmanned catamaran is designed. The seawater sample collection system is designed based on the data of the catamaran stability analysis model. The system can be analyzed respectively into four parts, including an auto-telescopic holding mechanism, a wire rope arranging mechanism, a double-body unmanned surface vehicle(USV) and a water docking diversion mechanism. Based on the classification, the internal components of the system are subjected to force analysis calculation and three-dimensional modeling by software, and the finite element analysis is conducted on the key points of the device. Therefore, the deep seawater samples collection system can meet the automatic deepwater collecting demand of USV under the influence of environmental factors such as wind, sea waves and surges.

In order to improve the hydrothermal deoxygenation of organic acids as well as to reduce the cost, we prepared a Cu-Ce/γ-Al2O3 catalyst to study the hydrothermal deoxygenation of stearic acid in the absence of H2. The Brunauer-Emmett-Teller surface area and X-ray diffraction pattern suggest that both CuO and CeO2 exist in the Cu-Ce/γ-Al2O3 catalyst. The crystals of Cu-Ce/γ-Al2O3 were more stable compared with those of the Cu/γ-Al2O3 catalyst after 12 h of hydrothermal liquefaction at 300℃, thereby indicating a better thermal stability of the former. The presence of Cu-Ce/γ-Al2O3 catalyst could greatly improve the conversion of stearic acid (94.71%) and the yield of hydrocarbon (81.41%). A preliminary analysis of the mechanism suggests that decarboxylation is the main step in the deoxygenation during the hydrothermal liquefaction of stearic acid. The addition of Cu/γ-Al2O3 and Cu-Ce/γ-Al2O3 decreased the yield of n-heptadecane and increased the yield of n-octadecane. Besides, the yields of n-paraffins increased drastically from 0.45% to 49.72% along the series from n-nonane to n-hexadecane. Thus, the cracking reactions could be improved in the presence of Cu-Ce/γ-Al2O3, suggesting that it is an efficient deoxygenation catalyst in the hydrothermal liquefaction of organic acid. In addition, the Cu-Ce/γ-Al2O3 catalyst could help in removing the carbonyl groups, and this effectively reduced the amounts of aldehydes and ketones in the bio-oil.