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6. [Characterization of β-1, 4-mannanase from Bacillus pumilus and heterologous expression in Lactobacillus casei].

Author

Zou Y, Lin J, Bu X, Jiang L, Chen Z, and Ge X

Subjects
Bacillus genetics, Bacterial Proteins metabolism, Cloning, Molecular, Enzyme Stability, Kinetics, Lacticaseibacillus casei metabolism, beta-Mannosidase metabolism, Bacillus enzymology, Bacterial Proteins chemistry, Bacterial Proteins genetics, Gene Expression, Lacticaseibacillus casei genetics, beta-Mannosidase chemistry, beta-Mannosidase genetics
Abstract

Objective: Lactobacillus casei is widely used in food production and feed industry. The aim of this study was to construct the recombinant expression mannanase Lb. casei., Methods: The mature peptide gene of β-1,4-mannanase from Bacillus pumilus was cloned into expression vectors pELX1 and pELSH, then electroporated into Lb. casei, establishing an intracellular and a secretion expression mannanase Lb. casei respectively., Results: After incubation, the specific activity of β-1,4-mannanase was 23 U/mg whole cell protein for intracellular expression and 8.8 U/mL for secretion expression in supernatant., Conclusion: Mannanase gene expression in Lb. casei provides application prospect and deserves further study.

Published
2015

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