Shi, Guolin, Chang, Zhuo, Zhang, Pan, Zou, Xiaohang, Zheng, Xinmin, Liu, Xiru, Yan, Jinxiao, Xu, Huiyun, Tian, Zhenhao, Zhang, Nu, Cui, Ning, Sun, Leming, Xu, Guangkui, and Yang, Hui
The bone marrow (BM) niches are the complex microenvironments that surround cells, providing various external stimuli to regulate a range of haematopoietic stem cell (HSC) behaviours. Recently, it has been proposed that the fate decision of HSCs is often correlated with significantly altered biophysical signals of BM niches. To thoroughly elucidate the effect of mechanical microenvironments on cell fates, we constructed 2D and 3D cell culture hydrogels using polyacrylamide to replicate the mechanical properties of heterogeneous sub‐niches, including the inherent rigidity of marrow adipose tissue (2 kPa), perivascular tissue (8 kPa) and endosteum region (35 kPa) in BM. Our observations suggest that HSCs can respond to the mechanical heterogeneity of the BM microenvironment, exhibiting diversity in cell mechanics, haematopoietic pool maintenance and differentiated lineages. Hydrogels with higher stiffness promote the preservation of long‐term repopulating HSCs (LT‐HSCs), while those with lower stiffness support multi‐potent progenitors (MPPs) viability in vitro. Furthermore, we established a comprehensive transcriptional profile of haematopoietic subpopulations to reflect the multipotency of haematopoietic stem and progenitor cells (HSPCs) that are modulated by niche‐like stiffness. Our findings demonstrate that HSPCs exhibit completely distinct downstream differentiated preferences within hydrogel systems of varying stiffness. This highlights the crucial role of tissue‐specific mechanical properties in HSC lineage decisions, which may provide innovative solutions to clinical challenges. [ABSTRACT FROM AUTHOR]