Search

The Botia superciliaris is endemic to China and distributed mainly in the upper reaches of the Yangtze River. The complete mitochondrial genome was 16,597 bp in length, which contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 displacement loop (D-loop) locus. Except for the ND6 gene and eight tRNA genes, all other mitochondrial genes were encoded on the heavy strand. The overall nucleotide composition was 31.6% A, 25.5% T, 27.2% C and 15.7% G, with an AT content of 57.1%. The gene composition and the structural arrangement of the B. superciliaris complete mtDNA were identical to most of the other vertebrates. The molecular data here we presented could play a useful role to study the evolutionary relationships and population genetics of Cobitidae fish. [ABSTRACT FROM AUTHOR]

The polymerase chain reaction method was employed to obtain the complete mitochondrial genome of Leiocassis crassilabris from the Yangtze River, China. The mitogenome of L. crassilabris is 16,534 bp in length, consisting of 37 genes coding for 13 proteins, 22 transfer RNAs, 2 ribosomal RNAs, and 1 control region. Its gene arrangement pattern was identical with that of most vertebrates. Overall base composition of mitogenome is estimated to be 31.8% A, 26.9% T, 26.4% C, and 14.9% G, with a high A+T content (58.7%). This is the first report on the complete mitogenome sequence of L. crassilabris, which can provide a basic data for the studies on phylogenetic analysis and conservation genetics. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome sequence of triploidpengzecrucian carp was 16,580 bp in length. The mitochondrial genome contains 22 transfer RNAs, 13 protein-coding genes, 2 ribosomal RNAs and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition is A 31.53%, T 26.23%, G 16.15%, C 26.09%, with an A + T bias of 57.76%. The complete mtDNA sequence of the geographic triploidpengzecrucian carp was reported in this study for the first time. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome was sequenced from the tetraploidCarassius auratus gibelioin this study. The genome sequence was 16,576 bp in length. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except forND6and eight tRNA genes. The overall base composition is 31.61% A, 25.81% T, 26.62% G, 15.96% C, with an A+T bias of 57.42%. The complete mitogenome data provides useful genetic markers for the studies on the molecular identification, population genetics, phylogenetic analysis and conservation genetics. [ABSTRACT FROM AUTHOR]

The marble goby is a carnivorous fish native to freshwater in Asia-Pacific region, and it is marketed as a high-valued fish in some Asia countries such as China, Thailand and Vietnam. This study presents the complete mitochondrial genome ofO. marmorata, which is 16,525 bp length with the genome organization and gene order being identical to that of the typical vertebrate. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome was sequenced from the freshwater fish,Pseudobagrus medianalis(Siluriformes: Bagridae) in this study. The genome sequence was 16,647 bp in length, and the gene order and contents were identical with the bagridae fishes. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except forND6and eight tRNA genes. The overall base composition is 25.7% A, 30.9% T, 28.0% G, 15.4% C, with an A+T bias of 56.6%. The complete mitogenome data provides useful genetic markers for the studies on the molecular identification, population genetics, phylogenetic analysis and conservation genetics. [ABSTRACT FROM AUTHOR]

We sequenced the complete mitogenomes of (Cyprinus carpio haematopterus) and Russian scattered scale mirror carp (Cyprinus carpio carpio). Comparison of these two mitogenomes revealed that the mitogenomes of these two common carp strains were remarkably similar in genome length, gene order and content, and AT content. There were only 55 bp variations in 16,581 nucleotides. About 1 bp variation was located in rRNAs, 2 bp in tRNAs, 9 bp in the control region and 43 bp in protein-coding genes. Furthermore, forty-three variable nucleotides in the protein-coding genes of the two strains led to four variable amino acids, which were located in the ND2, ATPase 6, ND5 and ND6 genes, respectively. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genomes of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis) were sequenced. Comparison of these two mitochondrial genomes revealed that the mtDNAs of these two common carp varieties were remarkably similar in genome length, gene order and content, and AT content. However, size variation between these two mitochondrial genomes presented here showed 39 site differences in overall length. About 2 site differences were located in rRNAs, 3 in tRNAs, 3 in the control region, 31 in protein-coding genes. Thirty-one variable bases in the protein-coding regions between the two varieties mitochondrial sequences led to three variable amino acids, which were mainly located in the protein ND5 and ND4. [ABSTRACT FROM AUTHOR]

In this study, the complete mitochondrial DNA (mtDNA) sequence of Pseudobagrus truncatus (Siluriformes: Bagridae) was determined. The complete mtDNA genome sequence of P. truncatus is 16,533 bp in size. It consists of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and one non-coding control region. The gene order and genes were the same as that found in other previously reported catfishes. The overall-based composition was 31.6% A, 26.7% T, 14.9% G and 26.8% C, with a high A + T content (58.3%). This complete mitogenome of P. truncatus provides a basic data for studies on species identification, molecular systematics and conservation genetics. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome of Pseudobagrus brevicaudatus (Siluriformes: Bagridae) was sequenced in this study. The total length of the mitogenome is 16,533 bp, with the base composition of 31.6% A, 26.8% T, 15.0%G, 26.6% C. The gene order and genes were the same as that found in other previously reported catfishes, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding control region. Except for ND6 gene and 8 tRNA genes, all other mitochondrial genes were encoded on the heavy strand. This complete mitogenome data provides the basis for taxonomic and conservation research of this and closely related species. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome of Shorthead catfish ( Pelteobagrus eupogon) was obtained by DNA sequencing based on the PCR fragments. The entire genome was 16,562 bp in length, with a gene content of 13 protein-coding, 2 ribosomal RNA, and 22 transfer RNA genes, and order identical to that observed in most other vertebrates. Those genes are arranged compactly with no introns and few intergenic nucleotides. Molecular data here presented provide a useful toll for evolutionary as well as population genetic studied. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome was sequenced from the tetraploid Carassius auratus gibelio in this study. The genome sequence was 16,576 bp in length. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition is 31.61% A, 25.81% T, 26.62% G, 15.96% C, with an A+T bias of 57.42%. The complete mitogenome data provides useful genetic markers for the studies on the molecular identification, population genetics, phylogenetic analysis and conservation genetics.

The marble goby is a carnivorous fish native to freshwater in Asia-Pacific region, and it is marketed as a high-valued fish in some Asia countries such as China, Thailand and Vietnam. This study presents the complete mitochondrial genome of O. marmorata, which is 16,525 bp length with the genome organization and gene order being identical to that of the typical vertebrate.

The complete mitochondrial genomes of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis) were sequenced. Comparison of these two mitochondrial genomes revealed that the mtDNAs of these two common carp varieties were remarkably similar in genome length, gene order and content, and AT content. However, size variation between these two mitochondrial genomes presented here showed 39 site differences in overall length. About 2 site differences were located in rRNAs, 3 in tRNAs, 3 in the control region, 31 in protein-coding genes. Thirty-one variable bases in the protein-coding regions between the two varieties mitochondrial sequences led to three variable amino acids, which were mainly located in the protein ND5 and ND4.

The complete mitochondrial genome was sequenced from the freshwater fish, Pseudobagrus medianalis (Siluriformes: Bagridae) in this study. The genome sequence was 16,647 bp in length, and the gene order and contents were identical with the bagridae fishes. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition is 25.7% A, 30.9% T, 28.0% G, 15.4% C, with an A+T bias of 56.6%. The complete mitogenome data provides useful genetic markers for the studies on the molecular identification, population genetics, phylogenetic analysis and conservation genetics.

The complete mitochondrial genome sequence of triploid pengze crucian carp was 16,580 bp in length. The mitochondrial genome contains 22 transfer RNAs, 13 protein-coding genes, 2 ribosomal RNAs and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition is A 31.53%, T 26.23%, G 16.15%, C 26.09%, with an A + T bias of 57.76%. The complete mtDNA sequence of the geographic triploid pengze crucian carp was reported in this study for the first time.

We sequenced the complete mitogenomes of (Cyprinus carpio haematopterus) and Russian scattered scale mirror carp (Cyprinus carpio carpio). Comparison of these two mitogenomes revealed that the mitogenomes of these two common carp strains were remarkably similar in genome length, gene order and content, and AT content. There were only 55 bp variations in 16,581 nucleotides. About 1 bp variation was located in rRNAs, 2 bp in tRNAs, 9 bp in the control region and 43 bp in protein-coding genes. Furthermore, forty-three variable nucleotides in the protein-coding genes of the two strains led to four variable amino acids, which were located in the ND2, ATPase 6, ND5 and ND6 genes, respectively.

Myostatin (MSTN) gene has negative influence on the growth of muscle, which belongs to transforming growth factor-β family. Using single-strand conformation polymorphism (SSCP), single nucleotide polymorphisms (SNPs) of MSTN gene were detected in yellow catfish. Correlation analysis between MSTN polymorphisms and growth traits was also carried out. Results indicated that five genotypes (AA, BB, CC, CD and DD) were found in the first intron with one deletion site and two mutant sites (T1003del, G1022A and T1063G), two genotypes (EE and EF) were found in the third exon with one mutant site (T132C). The individuals with genotypes AA had a lager value than the individuals with the genotype CD and DD in total length, body length, body height, body width, head length and body weight (Plt;0.05). The female individuals with genotypes AA also had a lager value than the female individuals with genotypes DD in total length, body length, body height, body width, caudal peduncle height, caudal peduncle width and body weight (Plt;0.05). This implies that genotype AA has a positive influence on the growth traits of female yellow catfish, and genotype DD is opposite. Genotypes AA and DD can be used in marker assisted selection of female yellow catfish breeding in the future.

Morphological classification of Bagridae fishes is relatively difficult due to various kinds and similar shape, and the phyletic evolution is not very clear in some species. To provide basic data to the classification of Bagridae and Siluriformes fishes, the complete mitochondrial genome of Pelteobagrus vachelli was obtained by PCR based on 16 primers, which were designed on the basis of related species mtDNA sequences. The complete mitochondrial genome is 16 527 bp in length, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes, and a non-coding control region. The organization and location of genes in the mitochondrial genome of Pelteobagrus vachelli were consistent with Siluriformes fishes published in GenBank. It has high homology with other families, such as Pseudobagrus Bleeker (91%) within Siluriformes. The phylogenetic analysis of Bagridae fishes was made from the level of mitochondria genome based on 9 species of 6 genera, which belong to 4 families of the Siluriformes, combining with 3 outgroups' complete mitochondrial genomes sequences. The result indicated that Pelteobagrus vachelli, Pelteobagrus fulvidraco, Pelteobagrus nitidus and Pseudobagrus tokiensis of the Bagridae formed a monophyletic group; the Pseudobagrus Bleeker and Pelteobagrus Bleeker formed a sister group. Moreover, Pelteobagrus vachelli was more relative to Pelteobagrus nitidus than Pelteobagrus fulvidraco in Pelteobagrus Bleeker.