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Globalization and climate change have intensified the need to address the marginalization of R&D for neglected and zoonotic diseases. We propose that drug repurposing, using enabling technologies such as artificial intelligence, can address this need at a lower cost than de novo R&D processes. [ABSTRACT FROM AUTHOR]

The COVID-19 pandemic has exposed critical weaknesses in the global health system, highlighting the urgent need for a coordinated international approach to pandemic prevention and management. As negotiations for a new WHO pandemic treaty progress, the effective integration of animal welfare is crucial. This paper aims to investigate the perspectives of key civil society organizations on the integration of animal welfare provisions into the pandemic treaty. Through a thematic analysis of documents prepared by FOUR PAWS, Wildlife Conservation Society, and Action for Animal Health between 2020–2023, five major themes are identified: prevention of zoonotic spillover, One Health approach, animal health systems and infrastructure, sustainable and ethical animal management practices, and policy coherence and governance. A comparative analysis of these themes against the April 2024 draft of the pandemic treaty reveals areas of alignment and divergence. Due to the ongoing controversies and the need for further improvements, the WHO's intergovernmental negotiating body was unable to finalize the treaty text for the 77th World Health Assembly in May 2024, leading to an extended mandate until 2025. Based on the findings, the paper proposes recommendations to strengthen the integration of animal welfare into the treaty, arguing that incorporating these recommendations is critical for developing a transformative, equitable, and effective treaty that addresses the systemic drivers of pandemic risk. [ABSTRACT FROM AUTHOR]

Background: Borneo, the third largest island in the world, is facing a significant burden of emerging and re-emerging vector-borne diseases due to rapid changes in primary tropical rainforests and urban landscapes. These vector-borne diseases include the endemic epidemic cycles that occur in the more populated and urbanized areas, as well as the possible transmission through enzootic and sylvatic transmission cycles that occur mainly in the overlapping landscapes or among the indigenous population in the forest. The island will be changed significantly in the future due to the increase in human activities, especially mega events such as the relocation of the Indonesian capital to Nusantara in East Kalimantan Borneo, increasing urbanization, agriculture, hydropower projects, ecotourism activities in Sabah, North Borneo, and Sarawak, Central and South Borneo. Therefore, an overview of the current situation of vector-borne diseases is crucial for the next possible epidemic preparedness. Methods: This study conducted the PRISMA-ScR scoping review and formulated a set of research questions to identify current trends in vector-borne diseases in Borneo. These questions aim to identify which diseases have been studied, what geographical areas have been covered by the research, how the One Health approach—encompassing human, animal and environmental factors—is integrated, and what gaps and challenges exist in addressing these diseases. Results: A total of 2241 references were screened for eligibility and 117 articles were selected for review. The majority of the materials focused on mosquitoes and malaria, and the One Health elements focused mainly on humans. Conclusions: This review has identified the most and least studied vector-borne diseases and highlighted some of the gaps in knowledge and research on vector-borne diseases on the island of Borneo. Future studies should particularly focus on other neglected diseases such as Zika, chikungunya, Japanese encephalitis, filariasis and tick-borne diseases. In addition, advanced surveillance systems will be developed to improve early detection and response specifically for remote regions where vector-borne diseases are endemic or emerging. [ABSTRACT FROM AUTHOR]

Simple Summary: This work provides an overview on the impact of bats' diet on their intestinal microorganism community and its influence on potential pathogenicity. Human expansion generates natural habitat alterations, which force bats to seek alternative sites, leading to contact with other animals, including humans. Exploring the relationship between the dietary habits of bats and the variety of their microbiome could contribute elucidating the potential role of bats as reservoirs of multidrug-resistant microorganisms and their implications in the dissemination of mutating viruses and antibiotic-resistant bacteria in the environment and possibly in their transmission to human and to domestic and wild animals. In this review, we tentatively tried to connect the most recent findings on the bat microbiome and to investigate on their microbial communities, that may vary even in conspecific hosts and are influenced by host physiology, feeding behavior and diet, social interactions, but also by habitat diversity and climate change. From a conservation perspective, understanding the potentially negative and indirect effects of habitat destruction on animal microbiota can also play a crucial role in the conservation and management of the host itself. According to the One Health concept, which recognizes an interdependence between humans, animals, and the environment, bat microbiota represents an indicator of host and environmental health, besides allowing for evaluation of the risk of emerging infectious diseases. We noticed that a growing number of studies suggest that animal microbiota may respond in various ways to changes in land use, particularly when such changes lead to altered or deficient food resources. We have highlighted that the current literature is strongly focused on the initial phase of investigating the microbial communities found in Chiroptera from various habitats. However, there are gaps in effectively assessing the impacts of pathogens and microbial communities in general in animal conservation, veterinary, and public health. A deeper understanding of bat microbiomes is paramount to the implementation of correct habitat and host management and to the development of effective surveillance protocols worldwide. [ABSTRACT FROM AUTHOR]

The patient was a girl (case 1) and her mother (case 2). The family had purchased a domestic cat five months previously. Three months later, both patients developed eruptions. Mycological examinations were positive in both cases, and the cat tested positive on the hairbrush test. The macroconidia were thick only in the cat strain, and drug susceptibility testing showed mildly low levels of terbinafine and itraconazole. However, a molecular biological analysis of these three strains showed 100% identity with reference strains of Microsporum canis. Since there have been recent reports of drug-resistant dermatophytosis, drug-susceptibility testing is considered necessary. [ABSTRACT FROM AUTHOR]

According to the WHO, more than 90,000 cases of mpox have been reported since the 2022 worldwide outbreak, which resulted in 167 deaths, while a new outbreak in Africa since 2023 has resulted in over 18,000 cases and 617 deaths. Mpox is a zoonosis caused by the monkeypox virus, a double-stranded DNA virus belonging to the Orthopoxvirus genus, which causes smallpox-like illness. Until 2022, cases were predominately located in West and Central Africa, with only sporadic cases and outbreaks reported in other parts of the world. During the 2022 outbreak, the primary mode of transmission was sexual contact among men who have sex with men. The changing epidemiology of mpox resulted in new disease phenotypes and populations at risk, disproportionally affecting people who live with HIV. Commonly presenting as a mild, self-limiting illness, mpox can cause severe and protracted disease in people with HIV with a CD4 count < 200 cell/mm3. The global emergence of mpox that followed and intersected with COVID-19 mobilized the scientific community and healthcare stakeholders to provide accurate diagnostics, preventive vaccines and treatment to those most affected. Despite existing gaps, this rapid response helped to contain the outbreak, but challenges remain as new variants emerge. Preparedness and readiness to respond to the next outbreak is crucial in order to minimize the impact to the most vulnerable. [ABSTRACT FROM AUTHOR]

Climate change is increasingly recognized as a significant driver of ecological and public health changes, particularly concerning vector-borne diseases. This scoping review aims to systematically map the current research on the impact of climate change on vector ecology and the subsequent effects on disease transmission dynamics. We conducted a comprehensive literature review across multiple databases to identify critical vectors, such as mosquitoes, ticks, and fleas. We examined how climate variables like temperature, precipitation, and humidity affect their populations, behaviors, and life cycles. Additionally, we explored the shifting geographic distributions of these vectors, investigating how climate change influences their spread and the emergence of diseases such as malaria, dengue, and Lyme disease in new regions. The review highlights the complex and multifaceted interactions between climate change and vector-borne diseases, emphasizing the necessity of understanding these relationships to inform effective public health strategies. Our findings indicate considerable variability in the impacts of climate change across different regions and vector species, underscoring the need for localized studies and tailored interventions. Moreover, significant research gaps were identified, particularly in predictive modeling, long-term surveillance, and the socio-economic impacts of vector-borne diseases exacerbated by climate change. We suggest directions for future research, including the development of integrated climate-health models and enhanced disease surveillance systems, to better anticipate and mitigate the effects of climate change on vector-borne disease transmission. This review underscores the urgency of addressing climate change as a critical component of global health initiatives and the importance of interdisciplinary approaches in tackling this complex issue. [ABSTRACT FROM AUTHOR]

The bond between humans and dogs is precious and has been treasured since ancient times. Dog ownership is linked to numerous health benefits, such as increased physical activity and social functioning and decreased depression and cardiovascular events. However, dogs can transmit zoonotic diseases to humans, many of which present with cutaneous findings. This review summarizes the dermatologic manifestations, transmission routes, diagnosis, and treatment of zoonotic diseases transmitted by dogs, including vector‐borne, bacterial, viral, fungal, and parasitic infections. This review emphasizes the significance of clinicians obtaining a comprehensive exposure history when patients exhibit a rash of unknown origin. Such an approach can provide valuable epidemiological clues related to diagnosing a zoonotic disease transmitted by a pet dog. Furthermore, identifying the dog as an infection source and subsequent veterinary treatment can help prevent recurrent infections in dermatologic patients. [ABSTRACT FROM AUTHOR]

Dengue and yellow fever have complex cycles, involving urban and sylvatic mosquitoes, and non‐human primate hosts. To date, efforts to assess the effect of climate change on these diseases have neglected the combination of such crucial factors. Recent studies only considered urban vectors. This is the first study to include them together with sylvatic vectors and the distribution of primates to analyse the effect of climate change on these diseases. We used previously published models, based on machine learning algorithms and fuzzy logic, to identify areas where climatic favourability for the relevant transmission agents could change: 1) favourable areas for the circulation of the viruses due to the environment and to non‐human primate distributions; 2) the favourability for urban and sylvatic vectors. We obtained projections of future transmission risk for two future periods and for each disease, and implemented uncertainty analyses to test for predictions reliability. Areas currently favourable for both diseases could keep being climatically favourable, while global favourability could increase a 7% for yellow fever and a 10% increase for dengue. Areas likely to be more affected in the future for dengue include West Africa, South Asia, the Gulf of Mexico, Central America and the Amazon basin. A possible spread of dengue could take place into Europe, the Mediterranean basin, the UK and Portugal; and, in Asia, into northern China. For yellow fever, climate could become more favourable in Central and Southeast Africa; India; and in north and southeast South America, including Brazil, Paraguay, Bolivia, Peru, Colombia and Venezuela. In Brazil, favourability for yellow fever will probably increase in the south, the west and the east. Areas where the transmission risk spread is consistent to the dispersal of vectors are highlighted in respect of areas where the expected spread is directly attributable to environmental changes. Both scenarios could involve different prevention strategies. [ABSTRACT FROM AUTHOR]

Terrestrial mollusks, including the invasive giant African snail (Lissachatina fulica), pose significant public health risks due to their role as carriers of various pathogens, such as Angiostrongylus cantonensis and Angiostrongylus costaricensis. This scoping review aims to provide a comprehensive evaluation of diseases associated with Lissachatina fulica and other terrestrial mollusks, with a particular focus on the prevention, diagnosis, and treatment of these conditions. Following the Joanna Briggs Institute guidelines and the PRISMA-ScR framework, we conducted a systematic search and filtered results, identifying 27 relevant case reports and series for analysis. Our findings reveal that ingesting raw or undercooked snails is the most hazardous exposure route, with a 75% mortality rate in affected adults, particularly in regions where snail consumption is culturally significant, such as France. A. cantonensis is the primary cause of eosinophilic meningitis, while A. costaricensis leads to abdominal angiostrongyliasis. The review also highlights the widespread impact of L. fulica in countries like France, the United States, Brazil, and Colombia, emphasizing the global nature of the threat. Children show consistent vulnerability across all exposure types, underscoring the need for targeted preventive strategies. This review underscores the urgent need for public health interventions, particularly educational campaigns to inform communities about the dangers of L. fulica. Additionally, it highlights the importance of enhancing diagnostic methods and expanding surveillance to better manage the risks associated with these invasive snails. The findings provide valuable insights for the scientific community and recommend a multidisciplinary approach to effectively mitigate the public health risks posed by L. fulica across diverse regions. [ABSTRACT FROM AUTHOR]

Abstract Background Borneo, the third largest island in the world, is facing a significant burden of emerging and re-emerging vector-borne diseases due to rapid changes in primary tropical rainforests and urban landscapes. These vector-borne diseases include the endemic epidemic cycles that occur in the more populated and urbanized areas, as well as the possible transmission through enzootic and sylvatic transmission cycles that occur mainly in the overlapping landscapes or among the indigenous population in the forest. The island will be changed significantly in the future due to the increase in human activities, especially mega events such as the relocation of the Indonesian capital to Nusantara in East Kalimantan Borneo, increasing urbanization, agriculture, hydropower projects, ecotourism activities in Sabah, North Borneo, and Sarawak, Central and South Borneo. Therefore, an overview of the current situation of vector-borne diseases is crucial for the next possible epidemic preparedness. Methods This study conducted the PRISMA-ScR scoping review and formulated a set of research questions to identify current trends in vector-borne diseases in Borneo. These questions aim to identify which diseases have been studied, what geographical areas have been covered by the research, how the One Health approach—encompassing human, animal and environmental factors—is integrated, and what gaps and challenges exist in addressing these diseases. Results A total of 2241 references were screened for eligibility and 117 articles were selected for review. The majority of the materials focused on mosquitoes and malaria, and the One Health elements focused mainly on humans. Conclusions This review has identified the most and least studied vector-borne diseases and highlighted some of the gaps in knowledge and research on vector-borne diseases on the island of Borneo. Future studies should particularly focus on other neglected diseases such as Zika, chikungunya, Japanese encephalitis, filariasis and tick-borne diseases. In addition, advanced surveillance systems will be developed to improve early detection and response specifically for remote regions where vector-borne diseases are endemic or emerging.

Abstract Bats are known reservoirs for a wide range of pathogenic microorganisms, including viruses, bacteria, fungi, helminths, and protozoa, which can be transmitted and infect other zoonotic organisms. Various studies have utilised next-generation sequencing (NGS) to describe the pathogens associated with bats. Although most have characterised microbial communities in specific body fluids, few have analysed the composition and diversity of these microbial communities across different body fluids at the individual level. In this study, we employed two next-generation sequencing techniques: amplicon-based sequencing of the V4 hypervariable region of the 16S- and 18S-rRNA genes and viral metagenomics, to describe the prokaryotic, eukaryotic, and viral communities present in blood, faeces, and oral swab samples collected from two genera of bats (Carollia and Phyllostomus) in the department of Casanare, eastern Colombia. A total of 60 samples corresponding to the three bodily fluids were processed and analysed. The results indicated that the microbial communities across the body fluids were mainly composed of bacteria, fungi, protozoa, and various DNA and RNA viruses, showing a variability of microbial genera and species. The abundances, diversity metrics, and correlations of these microorganisms displayed patterns associated with bat genus and body fluids, suggesting that the ecological characteristics of these microbial communities may be influenced by the ecological and physiological traits of the bats. Additionally, we found similar community compositions of bacteria, some fungal genera, and viruses in the three body fluids, indicating a possible circulation of these microbes within the same bat. This could be due to microbial movement from the gut microbiota to other physiological systems or transmission via blood-feeding vectors. Furthermore, our results revealed the presence of various microbes of public health concern, including Bartonella spp., Mannheimia haemolytica, Rhodotorula spp., Piroplasmida spp., Toxoplasma gondii, Alphacoronavirus spp., and Bat circovirus. The abundance of these pathogenic microbial species across the three bodily fluids suggests potential transmission routes from bats to other organisms, which may contribute to the emergence of zoonotic disease outbreaks. These findings highlight the variability of microorganisms present within the same bat and the different pathogen-host interactions that may regulate the presence and transmission of these zoonotic microbes. Further research is required to elucidate the genomic features, ecological interactions, and biological activities of these microbial communities in bats.

Wastewater-based epidemiology (WBE) has emerged as a valuable surveillance tool for SARS-CoV-2 and other pathogens globally, providing insights into community-level infections, including asymptomatic and pre-symptomatic cases. While most WBE programmes focus on quantitative pathogen assessment, next-generation sequencing (NGS) approaches have enabled more detailed analyses, including variant and recombinant genotype identification for viruses like SARS-CoV-2 and poliovirus. Despite recent NGS advancements allowing for the detection of known and novel viruses in wastewater, many of these tools remain underutilised in routine WBE. This short review critically evaluates the applicability of common NGS tools in routine WBE programmes, assessing their capability for identifying emerging threats with epidemic or pandemic potential. Here, we provide evidence-based recommendations for integrating NGS techniques into WBE and the use of results for informed decision-making within a One Health framework, aiming to enhance global infectious disease surveillance and pandemic preparedness. [ABSTRACT FROM AUTHOR]

A considerable amount of research has revealed that there exists an evolutionary mismatch between ancestral environments and conditions following the rise of agriculture regarding the contact between humans and animal reservoirs of infectious diseases. Based on this evolutionary mismatch framework, we examined whether visual attention exhibits adaptive attunement toward animal targets' pathogenicity. Consistent with our predictions, faces bearing heuristic infection cues held attention to a greater extent than did animal vectors of zoonotic infectious diseases. Moreover, the results indicated that attention showed a specialized vigilance toward processing facial cues connoting the presence of infectious diseases, whereas it was allocated comparably between animal disease vectors and disease-irrelevant animals. On the other hand, the pathogen salience manipulation employed to amplify the participants' contextual-level anti-pathogen motives did not moderate the selective allocation of attentional resources. The fact that visual attention seems poorly equipped to detect and encode animals' zoonotic transmission risk supports the idea that our evolved disease avoidance mechanisms might have limited effectiveness in combating global outbreaks originating from zoonotic emerging infectious diseases. [ABSTRACT FROM AUTHOR]

Objective: To examine the socio-environmental factors associated with the assemblage of small mammals and the prevalence of Leptospira pathogen in poor suburban communities of Terengganu, Malaysia. Methods: We trapped small mammals from 119 trapping points scattered around three suburban communities of Terengganu using sausage-baited live traps. On the average, we set up five traps for three nights at each sampling point during the trapping period. Kidneys of captured animals were harvested and processed for Leptospira investigation. Additionally, environmental survey was conducted at each trapping point to obtain information about possible variables supporting small mammal assemblage. We used a generalized linear model to evaluate the effect of different socio-environmental variables on small mammals’ assemblage. Results: A total of 89 small mammals, specifically, Rattus norvegicus (n=39), Rattus rattus (n=27), Rattus exulans (n=10), Suncus murinus (n=11), and Tupaia glis (n=2) were captured from 1 385 trap nights. Fourteen individuals (15.7%) of the captured animals tested positive for Leptospira bacteria using PCR detection. Results of our generalized linear model showed only residences bordering vacant lots as the variable positively associated with small mammal occurrence in the three study sites. Conclusions: Small mammal community, especially the often neglected species, could harbour and potentially contribute towards pathogenic Leptospira maintenance in the study sites. To adequately control small mammals’ population and subsequent human zoonoses transmission, it is critical to advocate and promote appropriate infrastructure and suburban services, together with good hygiene practices that can reduce the animals’ access to food and harborage.

Abstract Zoonotic diseases remain a persistent threat to global public health. Many major zoonotic pathogens exhibit seasonal patterns associated with climatic variations. Quantifying the impacts of environmental variables such as temperature and humidity on disease transmission dynamics is critical for improving prediction and control measures. This review synthesizes current evidence on the relationships between temperature and humidity and major zoonotic diseases, including malaria, dengue, rabies, anisakiasis, and influenza. Overall, this review highlighted some overarching themes across the different zoonotic diseases examined. Higher temperatures within suitable ranges were generally associated with increased transmission risks, while excessively high or low temperatures had adverse effects. Humidity exhibited complex nonlinear relationships, facilitating transmission in certain temperature zones but inhibiting it in others. Heavy rainfall and high humidity were linked to vector‐borne disease outbreaks such as malaria by enabling vector breeding. However, reduced incidence of some diseases like dengue fever was observed with high rainfall. To address existing knowledge gaps, future research efforts should prioritize several key areas: enhancing data quality through robust surveillance and the integration of high‐resolution microclimate data; standardizing analytical frameworks and leveraging advanced methodologies such as machine learning; conducting mechanistic studies to elucidate pathogen, vector, and host responses to climatic stimuli; adopting interdisciplinary approaches to account for interacting drivers; and projecting disease impacts under various climate change scenarios to inform adaptation strategies. Investing in these research priorities can propel the development of evidence‐based climate‐aware disease prediction and control measures, ultimately safeguarding public health more effectively.

Abstract Background In recent years, Babesia and Bartonella species co-infections in patients with chronic, nonspecific illnesses have continued to challenge and change the collective medical understanding of “individual pathogen” vector-borne infectious disease dynamics, pathogenesis and epidemiology. The objective of this case series is to provide additional molecular documentation of Babesia odocoilei infection in humans in the Americas and to emphasize the potential for co-infection with a Bartonella species. Methods The development of improved and more sensitive molecular diagnostic techniques, as confirmatory methods to assess active infection, has provided increasing clarity to the healthcare community. Results Using a combination of different molecular diagnostic approaches, infection with Babesia odocoilei was confirmed in seven people suffering chronic non-specific symptoms, of whom six were co-infected with one or more Bartonella species. Conclusions We conclude that infection with Babesia odocoilei is more frequent than previously documented and can occur in association with co-infection with Bartonella spp. Graphical Abstract

As society develops and public concern for animal protection grows, the perception and value of dogs is changing in China. In recent years, an increasing number of citizens have kept dogs for companionship and emotional support and have become concerned with the well-being of dogs. Despite changes in the perception of the place of dogs in Chinese society, the legal status of dogs has remained unchanged. Without the proper legal protection, dogs in China run numerous risks. In particular, as the novel coronavirus outbreak spread globally with devastating effects on human health, countless animals have become victims of "pandemic panic." The Covid-19 pandemic affected animal ethics and the human-animal relationship, which underwent significant changes. By tracking the evolution of policies on companion animal management, this study explores the current living conditions of companion animals in China, particularly during the Covid-19 pandemic, as well as social attitudes towards urban animal companions. [ABSTRACT FROM AUTHOR]

Objective: To examine the socio-environmental factors associated with the assemblage of small mammals and the prevalence of Leptospira pathogen in poor suburban communities of Terengganu, Malaysia. Methods: We trapped small mammals from 119 trapping points scattered around three suburban communities of Terengganu using sausage-baited live traps. On the average, we set up five traps for three nights at each sampling point during the trapping period. Kidneys of captured animals were harvested and processed for Leptospira investigation. Additionally, environmental survey was conducted at each trapping point to obtain information about possible variables supporting small mammal assemblage. We used a generalized linear model to evaluate the effect of different socio-environmental variables on small mammals' assemblage. Results: A total of 89 small mammals, specifically, Rattus norvegicus (n =39), Rattus rattus (n =27), Rattus exulans (n =10), Suncus murinus (n =11), and Tupaia glis (n =2) were captured from 1 385 trap nights. Fourteen individuals (15.7%) of the captured animals tested positive for Leptospira bacteria using PCR detection. Results of our generalized linear model showed only residences bordering vacant lots as the variable positively associated with small mammal occurrence in the three study sites. Conclusions: Small mammal community, especially the often neglected species, could harbour and potentially contribute towards pathogenic Leptospira maintenance in the study sites. To adequately control small mammals' population and subsequent human zoonoses transmission, it is critical to advocate and promote appropriate infrastructure and suburban services, together with good hygiene practices that can reduce the animals' access to food and harborage. [ABSTRACT FROM AUTHOR]

Background and Aims: Crimean‐Congo Hemorrhagic Fever (CCHF) is a significant public health concern transmitted by ticks. This study seeks to thoroughly grasp the epidemiology and transmission patterns of CCHF, which is caused by the CCHF virus (CCHFV), a member of the Nairovirus genus in the Bunyaviridae family. Methods: The study investigates the global distribution and endemicity of CCHF, its mortality rates, modes of transmission (including tick bites, contact with infected animal blood, and limited person‐to‐person transmission), and factors influencing its prevalence across different regions. Genetic diversity within CCHFV and its impact on transmission dynamics are explored, along with efforts to control the disease through tick prevention, antiviral treatment, and the development of vaccines and diagnostics. Results: CCHFV exhibits widespread distribution, particularly in the Middle East, Africa, Asia, and Eastern Europe, with an overall mortality rate of approximately 30% and a case fatality rate ranging from 10% to 40%. Transmission occurs primarily through tick bites and contact with infected animal blood, with limited person‐to‐person transmission. Livestock workers, slaughterhouse employees, and animal herders in endemic areas are most affected by their frequent interaction with sick animals and ticks. Genetic diversity within CCHFV contributes to variations in transmission dynamics, complicating control efforts. Antiviral ribavirin shows efficacy in treating CCHF infection. Conclusion: This study underscores the importance of further research to understand the enzootic environment, transmission routes, and genetic diversity of CCHFV for effective control measures, including the development of vaccines, treatment options, and diagnostics. Highlights: Crimean‐Congo Hemorrhagic Fever (CCHF) is prevalent in the Middle East, Africa, Asia, and Eastern Europe, with a mortality rate of ~30%.Transmission primarily occurs through tick bites and contact with infected animal blood, posing risks to livestock workers and animal herders.Genetic diversity within CCHFV influences its transmission dynamics, complicating control efforts.Antiviral ribavirin demonstrates efficacy in treating CCHF infections, highlighting potential therapeutic avenues. [ABSTRACT FROM AUTHOR]

The World Health Organization (WHO) estimates that many human infections are zoonoses, creating a worldwide public health challenge. Among Chlamydia species, Chlamydia felis is the leading cause of conjunctivitis in cats and is a prominent zoonotic species. This study aimed to determine the occurrence and risk of chlamydiosis in cats and dogs in Szeged, Hungary, and surrounding areas. The total nucleic acids from conjunctival swab samples of symptomatic and asymptomatic animals were extracted using an automated nucleic acid extraction system. After that, DNA was amplified by pan-chlamydia PCR. Bacterial and fungal cultures were also performed to detect other microorganisms. Of the 93 animals, 32 (34.4%) were positive for pan-chlamydia PCR. The positivity rates were 33.3% (26/78) in cats and 40.0% (6/15) in dogs. Furthermore, the positivity rates were 37.2% (16/43) in the cat shelter, 42.4% (14/33) in the veterinary clinic, and 11.7% (2/17) in household pets. In total, 103 species were identified through culture-based examinations, including 97 (94.2%) bacterial and 6 fungal (5.8%) species. From both human and animal health perspectives, it is essential to have a detailed understanding of the circumstances of chlamydiosis, given the global impact of zoonotic diseases. [ABSTRACT FROM AUTHOR]

Simple Summary: This paper reviews key issues regarding the spread of diseases that affect both humans and animals, known as zoonotic diseases. These diseases, which make up about 70% of all new and existing diseases, are increasingly interconnected with factors like the environment, society, and economics. The term "zoonoses" is discussed beyond just veterinary medicine, emphasizing its complex nature influenced by human activities and climate change. Bioethical principles and strategies for preventing these diseases are proposed. A case study on animal slaughter during disasters highlights ethical challenges in how we manage health across species, leading to discussions on "zoonoethics". The paper also explores how these diseases emerge and the ethical and political issues around antimicrobial resistance (AMR), with recommendations for tackling AMR. The management of these diseases will require the adoption and acceleration of the "One Health" approach, which recognizes the interconnectedness and interdependence between human, animal, and environmental health. This paper presents a critical review of key issues related to the emergence of new networks for the spread of zoonotic diseases amid the mass extinction of species. Zoonotic and infectious diseases account for approximately 70% of new and existing diseases affecting humans and animals. The initial section argues that the term "zoonoses" should not be confined to single-cause events within veterinary medicine. Instead, zoonoses should be viewed as complex, systemic phenomena shaped by interrelated factors, including environmental, sociocultural, and economic elements, influenced by anthropogenic climate change. The second section presents bioethical principles and potential strategies for those engaged in zoonotic disease prevention. The third section uses the slaughter of animals in disaster settings as a case study to illustrate the need for further clarification of normative and interspecies justice conflicts in One Health ethics. This section concludes with an outlook on "zoonoethics". Section four develops the analysis of the interlinked elements that trigger zoonoses and examines antimicrobial resistance (AMR) from an ethical and political standpoint, concluding with policy recommendations for addressing AMR. Section five offers a critical reflection, integrating contributions from zoonoethics, human ecology, and the ecotheological turn. Finally, section six concludes with a call to action and policy recommendations for an inclusive, intercultural, and gender-sensitive One Health approach. [ABSTRACT FROM AUTHOR]

Background: Bartonellosis, caused by bacteria of the genus Bartonella, is a zoonotic disease with several mammalian reservoir hosts. In Somalia, a country heavily reliant on livestock, zoonotic diseases pose significant public health and economic challenges. To the best of our knowledge, no study has been performed aiming to verify the occurrence of Bartonella spp. in Somalia. This study investigated the occurrence and molecular characterization of Bartonella in dromedary (Camelus dromedarius, Linnaeus, 1758), cattle, sheep, and goats from Somalia. Materials and Methods: 530 blood samples were collected from various animals (155 dromedary, 199 goat, 131 cattle, and 45 sheep) in Benadir and Lower Shabelle regions. DNA was extracted for molecular analysis, and a qPCR assay targeting the NADH dehydrogenase gamma subunit (nuoG) gene was used for Bartonella screening. Positive samples were also subjected to PCR assays targeting seven molecular markers including: nuoG, citrate synthase gene (gltA), RNA polymerase beta‐subunit gene (rpoB), riboflavin synthase gene (ribC), 60 kDa heat‐shock protein gene (groEL), cell division protein gene (ftsZ), and pap31 and qPCR targeting the 16‐23S rRNA internal transcribed spacer (ITS) followed by Sanger sequencing, BLASTn and phylogenetic analysis. Results: Out of 530 tested animals, 5.1% were positive for Bartonella spp. by the nuoG qPCR assay. Goats showed the highest Bartonella occurrence (17/199, 8.5%), followed by sheep (6/44, 6.8%), cattle (4/131, 3.1%), and dromedary (1/155, 1.9%). Goats, sheep, and cattle had higher odds of infection compared to dromedary. Among nuoG qPCR‐positive samples, 11.1%, 14.8%, 11.1%, and 25.9% were positive in PCR assays based on nuoG, gltA, and pap31 genes, and in the qPCR based on the ITS region, respectively. On the other hand, nuoG qPCR‐positive samples were negative in the PCR assays targeting the ribC, rpoB, ftsZ, and groEL genes. While Bartonella bovis sequences were detected in cattle (nuoG and ITS) and goats (gltA), Bartonella henselae ITS sequences were detected in dromedary, goat, and sheep. Phylogenetic analysis placed gltA Bartonella sequence from a goat in the same clade of B. bovis. Conclusion: The present study showed, for the first time, molecular evidence of Bartonella spp. in dromedary and ruminants from Somalia and B. henselae in sheep and goats globally. These findings contribute valuable insights into Bartonella spp. occurrence in Somali livestock, highlighting the need for comprehensive surveillance and control measures under the One Health approach. [ABSTRACT FROM AUTHOR]

The purpose of the study was to analyse the quality of vaccination among the population and to evaluate strategies that contribute to reducing the prevalence of infectious diseases, the number of complications, and the severity of disease. The study also examined pathogens that pose a global threat to the population, which have high risks of outbreaks due to global changes in climatic conditions. The development of vaccines capable of preventing or eliminating an infectious disease, reducing the severity of the disease and the rate of hospitalisation has been studied. In addition, the issue of the causes of low vaccination coverage in Kyrgyzstan, Albania, Bulgaria, and Latvia has been investigated. It was found that diseases that have a geographical distribution in certain climatic zones have a risk of zone expansion due to global climate warming and changes in the habitat of the pathogen or its vectors to other regions. Global health systems are constantly working to create new vaccines and modernise old ones. Despite this, there are many reasons why vaccination coverage is not reaching the target values. These reasons include the availability of vaccines to the public, the level of knowledge of medical personnel and the trust of doctors in vaccination, the level of education of the population and the availability of information about the vaccine, commitment to vaccination in patients, and trust in international medical health systems. This means that low vaccination coverage can lead to a decrease in collective immunity, the occurrence of outbreaks of infectious diseases, and an increase in the burden on the health care system. Therefore, the main strategy for immunisation of the population is to eliminate the causes of low vaccination coverage, take measures to inform the population about vaccines, and increase people's confidence in health systems. [ABSTRACT FROM AUTHOR]

Simple Summary: This review explores the safety of camel meat and the diseases that camels can transmit to humans in the Middle East conducted over the past ten years, emphasizing the need for a One Health approach. An examination of recent studies indicated significant issues with pathogens, including antibiotic-resistant bacteria and contamination with heavy metals and pesticides. The review also highlighted the ongoing risk of diseases like Middle East respiratory syndrome coronavirus (MERS-CoV) and other zoonoses. Findings from this review call for more robust food safety measures and increased cooperation among veterinary and public health authorities to ensure the safety of camel meat and protect public health. The purpose of this review was to investigatethe microbial and chemical safety of camel meat and the zoonotic diseases associated with camels in the Middle East over the past decade, emphasizing the crucial role of a One Health approach. By systematically analyzing recent studies (in the past decade, from 2014), we assessed pathogen prevalence, contamination with heavy metals and pesticide residues, and the impact of zoonotic diseases like Middle East respiratory syndrome coronavirus (MERS-CoV). The findings revealed significant variability in pathogen prevalence, with the frequent detection of traditional foodborne pathogens (e.g., Salmonella and E. coli O157), as well as antibiotic-resistant strains like methicillin-resistant and vancomycin-resistant Staphylococcus aureus and extended-spectrum β-lactamase (ESBL)-producing E. coli, underscoring the need for stringent antibiotic use policies and robust food safety measures. Additionally, the review highlighted substantial contamination of camel meat with heavy metals and pesticide residues, posing significant public health concerns that necessitate stringent regulatory measures and regular monitoring. The persistent occurrence of zoonotic diseases, particularly MERS-CoV, along with other threats like trypanosomiasis, brucellosis, and Clostridium perfringens, emphasizes the importance of strengthening ongoing surveillance. Enhancing investment in diagnostic infrastructures, training programs, and planning capabilities is crucial to address these issues at the camel–human interface in the Middle East. Adopting a One Health perspective is vital to ensuring the safety and quality of camel meat and managing zoonotic risks effectively to ultimately safeguard public health and promote sustainable livestock practices. [ABSTRACT FROM AUTHOR]

Background: In recent years, Babesia and Bartonella species co-infections in patients with chronic, nonspecific illnesses have continued to challenge and change the collective medical understanding of "individual pathogen" vector-borne infectious disease dynamics, pathogenesis and epidemiology. The objective of this case series is to provide additional molecular documentation of Babesia odocoilei infection in humans in the Americas and to emphasize the potential for co-infection with a Bartonella species. Methods: The development of improved and more sensitive molecular diagnostic techniques, as confirmatory methods to assess active infection, has provided increasing clarity to the healthcare community. Results: Using a combination of different molecular diagnostic approaches, infection with Babesia odocoilei was confirmed in seven people suffering chronic non-specific symptoms, of whom six were co-infected with one or more Bartonella species. Conclusions: We conclude that infection with Babesia odocoilei is more frequent than previously documented and can occur in association with co-infection with Bartonella spp. [ABSTRACT FROM AUTHOR]

Copyright of Revista Veterinaria is the property of Universidad Nacional del Nordeste and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: The prioritization of the One Health approach remains lacking in countries like Nepal, where the urgency to tackle this issue has yet to be acknowledged. It is imperative to incentivize rural farmers to enhance the well-being of their crops and livestock for the sake of fostering truly sustainable agriculture. This approach not only aligns with economic sensibility but also paves the way for a more enduring and resilient economy in the long run. By disseminating knowledge about One Health and executing the One Health National Strategy, Nepal stands to enhance its overall health landscape and set a precedent for other South Asian nations. Beyond immediate financial gains, stakeholders including farmers, policymakers, and consumers must adopt a forward-thinking perspective, integrating the One Health approach into their practices to facilitate a sustainable developmental trajectory. Research methods encompassed a thorough examination of One Health publications and the collection of data via a structured questionnaire administered during field visits, supplemented by telephone interviews with participants. The lessons gleaned from the COVID-19 pandemic underscore the significance of addressing health crises stemming from zoonotic diseases--those originating in animals and transferring to humans. Prioritizing the reinforcement of preparedness and response capabilities in rural settings, devising contingency plans, diversifying agricultural produce, investing in eco-friendly farming techniques, and promoting sustainable agricultural practices are pivotal for empowering communities to better withstand future adversities and uncertainties. [ABSTRACT FROM AUTHOR]

In recent decades, the global rise of viral emerging infectious diseases has posed a substantial threat to both human and animal health worldwide. The rapid spread and accumulation of mutations into viruses, and the limited availability of antiviral drugs and vaccines, stress the urgent need for alternative therapeutic strategies. Antimicrobial peptides (AMPs) derived from natural sources present a promising avenue due to their specificity and effectiveness against a broad spectrum of pathogens. The present study focuses on investigating the antiviral potential of oreochromicin‐1 (oreoch‐1), a fish‐derived AMP obtained from Nile tilapia, against a wide panel of animal viruses including canine distemper virus (CDV), Schmallenberg virus (SBV), caprine herpesvirus 1 (CpHV‐1), and bovine herpesvirus 1 (BoHV‐1). Oreoch‐1 exhibited a strong antiviral effect, demonstrating an inhibition of infection at concentrations in the micromolar range. The mechanism of action involves the interference with viral entry into host cells and a direct interaction between oreoch‐1 and the viral envelope. In addition, we observed that the peptide could also interact with the cell during the CDV infection. These findings not only highlight the efficacy of oreoch‐1 in inhibiting viral infection but also emphasize the potential of fish‐derived peptides, specifically oreoch‐1, as effective antiviral agents against viral infections affecting animals, whose potential to spill into humans is high. This research contributes valuable insights to the ongoing quest for novel antiviral drugs with the potential to mitigate the impact of infectious diseases on a global scale. [ABSTRACT FROM AUTHOR]

Copyright of Anuario Turismo y Sociedad is the property of Universidad Externado de Colombia, Facultad de Empresas Turísticas y Hoteleras and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

It is estimated that 60% of infectious diseases, and up to 75% of emerging and reemerging diseases are zoonotic. Inhabitants of tropical rural areas are the most affected by zoonotic diseases. However, knowledge of these inhabitants in vulnerable areas about zoonotic diseases is rarely known and taken into consideration for prevention programs and disease control. Semi–structured questionnaires were applied to 96 inhabitants of Mayan communities from the state of Yucatán, Mexico, to investigate their knowledge and risk perception of rabies, taeniasis/cysticercosis complex, trichinosis, toxoplasmosis, rickettsiosis, Chagas disease, leishmaniasis, leptospirosis and brucellosis. Rabies (88.5%) and Chagas disease (35.4%) were the most well–known diseases while taeniasis/cysticercosis complex (7.5%), brucellosis (3.1%), toxoplasmosis (1%) and trichinosis (1%) were the lesser–known diseases. None of the interviewees mentioned knowing rickettsiosis, leishmaniasis or leptospirosis. Of the inhabitants who had knowledge about rabies, 98.7% mentioned that the disease is spread by the bite of an animal, 60% indicated that the vaccination of animals prevents the disease, and 65% considered that this disease represents a high risk for health. Of the people who recognized Chagas disease, 93.7% mentioned that the disease is acquired through the bite of blood–sucking bugs, commonly referred to as “pics” in the Mayan language. In general, Chagas disease prevention measures were varied and infrequent, such as not touching “pics” (11.8%). Informative talks (46.9%) and television (29.2%) were the means through which they would like to receive information about diseases. Due to the inhabitants of tropical rural communities living with multiple socioeconomic and environmental conditions that increase the risk of exposure and transmission of zoonotic diseases, it is necessary to implement educational programs for control and prevention of zoonosis for short–, medium–, long–term that consider the knowledge, perceptions and necessities of the communities.

Free-ranging Chacma baboon species are known to harbour a wide range of zoonotic parasites, and their frequent close interactions with humans pose a risk of transmission of zoonotic parasites between the two species. This research study focused on understanding parasite dynamics in free-ranging baboon populations that inhabit human-wildlife interface areas, a case of Gwanda State University's Epoch Mine campus in Filabusi at Insiza district. A descriptive and analytical cross-sectional design was used to investigate the prevalence, diversity and association of gastrointestinal parasites among three baboon troops found at the Epoch Mine campus. One hundred and twenty (120) fresh faecal samples were collected from the three troops between February and September 2023. The centrifugal floatation technique was used to process and analyse faecal samples, and parasite ova and cysts were identified using morphological features like shape and size. The prevalence of the parasite taxa and a chi-squared multiple comparison test was computed. Results showed significant differences among most parasite taxa except Coccidia spp and Entamoeba spp among the three troops. The Shannon–Wiener (H′) index was used to calculate diversity and graphs were utilized to present the association. The Kruskal-Wallis tests showed no significant difference in parasite diversity among the three troops. Although troop-parasite association showed different parasite species for each troop, helminths Strongylid spp. and Schistosoma spp. were highly common across all three troops. Troop 1 and 3 were associated with four parasite taxa, whereas Troop 2 had only three. The study reveals the presence of gastro-intestinal parasites of public health concern, as they are capable of causing diseases in humans and domestic animals. The study, therefore, underscores the importance of understanding parasite-host dynamics in mitigating zoonotic disease transmission and suggests the need to generate baseline data for mitigating zoonotic diseases and maintaining a healthy ecosystem.

The USDA/ARS-National Disease Center (NADC) will celebrate its 65th anniversary of existence in November 2026. NADC continues as one of the world's premier animal health research centers conducting basic and applied research on endemic diseases with economic impact on U.S. livestock and wildlife. This research center also supports a program studying important food safety pathogens such as Salmonella, E. coli and Campylobacter. NADC has contributed significantly to the elimination of a few diseases, notably hog cholera and milk fever, and made progress in reducing the impact of many other animal diseases through vaccines, therapies and managerial recommendations. Despite nearly 65 years of targeted research on these diseases and much progress, some of these continue to persist. The reasons for such persistence varies for each disease condition and they are often multifactorial involving host susceptibility, virulence and even environmental conditions. Individually and in aggregate, these disease conditions have a massive economic impact and can be devasting to animal producers, owners and individuals that become infected through zoonotic disease agents such as tuberculosis, leptospirosis and avian influenza. They also diminish the health, well-being and welfare of affected animals, which directly affects the food supply. The NADC is using all available technologies including genomic, biochemical, reverse genetics, and vaccine trials in the target host to combat these significant diseases. We review the progress and reasons for persistence of selected diseases and food safety pathogens as well as the progress and potential outcomes should research and programmatic plans to eliminate these disease conditions cease.

Food insecurity is a serious and immediate concern for the world due to challenges including overpopulation; conflicts; animal, plant, and human diseases; climate change; depletion of resources; and environmental degradation. Long-term solutions for food production must consider the impacts on the environment, water and other resources, human and animal health, and sustainable crop production.The fundamental cause of food insecurity is the rapid and unprecedented increase in human population from approximately 2 billion people in 1925 to over 8 billion in 2023. The need for food has led to major expansion of both crop and food animal production including movement into new areas; increased production requires more resources, some of which are being depleted. This paper focuses on food animal production. Humans depend on three major species for most animal-origin food: Chickens (meat and eggs), cattle (meat and milk), and swine (meat). Each species is currently threatened by diseases that can rapidly spread internationally, and some have zoonotic potential. Diversification of animal-based food, such as expanding aquaculture can help to protect against food shortages should an epizootic occur in one or more of the above species.Cutting-edge science is needed to improve food animal production and pathogen control. This requires an interdisciplinary one-world, one-health approach led by international organizations and funded by the developed world. An optimal response will involve scientists and policy experts from government, the private sector, and universities worldwide. Strengthening all nations' public health infrastructure and veterinary services is essential to this aim.Fortunately, concerns about worldwide food security are concurrent with rapid advances in nearly all aspects of science, which can be applied to sustainably increase food production both locally and globally. There must be a collective will to apply science and to implement policies to solve current food security problems and to prepare for future challenges.

The COVID-19 pandemic has exposed critical weaknesses in the global health system, highlighting the urgent need for a coordinated international approach to pandemic prevention and management. As negotiations for a new WHO pandemic treaty progress, the effective integration of animal welfare is crucial. This paper aims to investigate the perspectives of key civil society organizations on the integration of animal welfare provisions into the pandemic treaty. Through a thematic analysis of documents prepared by FOUR PAWS, Wildlife Conservation Society, and Action for Animal Health between 2020–2023, five major themes are identified: prevention of zoonotic spillover, One Health approach, animal health systems and infrastructure, sustainable and ethical animal management practices, and policy coherence and governance. A comparative analysis of these themes against the April 2024 draft of the pandemic treaty reveals areas of alignment and divergence. Due to the ongoing controversies and the need for further improvements, the WHO's intergovernmental negotiating body was unable to finalize the treaty text for the 77th World Health Assembly in May 2024, leading to an extended mandate until 2025. Based on the findings, the paper proposes recommendations to strengthen the integration of animal welfare into the treaty, arguing that incorporating these recommendations is critical for developing a transformative, equitable, and effective treaty that addresses the systemic drivers of pandemic risk.

Abstract Background Traditional medicine (TM) interventions are plausible therapeutic alternatives to conventional medical interventions against emerging and endemic zoonotic diseases, particularly in low-and middle-income countries that may lack resources and infrastructure. Despite the growing popularity in the usage of TM interventions, their clinical safety and effectiveness are still contested within conventional healthcare in many countries. Methods We conducted a scoping review of the peer-reviewed literature that synthesises and maps the evidence on TM interventions for the treatment and prevention of zoonoses on the Indian subcontinent. The region, a global hotspot of biodiversity and emerging infections, is characterised by high prevalence of TM use. Based on the scientific literature (mostly case study research, n=l06 studies), our review (1) maps the scope of the literature, (2) synthesises the evidence on the application of TM interventions for zoonoses, and (3) critically reflects on the state of TM and identifies areas for future research focus. Results The evidence synthesis confirmed widespread usage of TM interventions for zoonoses on the subcontinent, with the majority of research reported from India (n=99 studies, 93.4%), followed by Pakistan (n=3 studies, 2.8%), Bangladesh (n=2 studies, 1.9%), and Sri Lanka (n=1, 0.9%). Most of the reviewed studies reported on ethno-medicinal uses of plant species, primarily for treating dengue (n=20 studies), tuberculosis (n=18 studies), Escherichia coli infection (n=16 studies), lymphatic filariasis and cholera (n=9 apiece). However, the evidence on the safety and effectiveness of these reported TM interventions is limited, indicating that these data are rarely collected and/or shared within the peer-reviewed literature. Conclusion This review thus highlights that, whilst TMs are already being used and could offer more widely accessible interventions against emerging and endemic zoonoses and ectoparasites, there is an urgent need for rigorous clinical testing and validation of the safety and effectiveness of these interventions.

Background & Aims: Visceral leishmaniasis, a potentially fatal disease caused by Leishmania infantum, is prevalent in the Mediterranean region and the Middle East. In Iran, it is endemic in the Northwest, Southwest, Northeast, Southeast, and central regions, with sporadic occurrences elsewhere. This study aimed to ascertain the period prevalence, epidemiological characteristics, and the most common clinical symptoms and laboratory signs of visceral leishmaniasis in North Khorasan Province. Materials & Methods: Over a nine-year period from 2010 to 2018, clinical records of visceral leishmaniasis cases were scrutinized to gather demographic and clinical data. Diagnosis was confirmed paraclinically through positive smears for Leishman bodies in bone marrow aspirates. Each patient completed a questionnaire. Results: A retrospective analysis was conducted on 24 diagnosed patients. Reported case numbers varied annually, with prevalence ranging from 0.0 to 1.1 per 100,000, peaking in 2017 and reaching its nadir in 2011. The majority of patients were hailed from rural areas (83.3%), with age distribution spanning from 8 months to 62 years. Most patients (95.8%) were under 10 years old, and 66.7% were male. Predominant clinical symptoms and laboratory signs included fever (91.7%), splenomegaly (79.2%), anemia (83.3%), thrombocytopenia (58.3%), and leukopenia (54.2%). Conclusion: The incidence of visceral leishmaniasis in North Khorasan Province surpasses the national average by threefold, indicating its significance as an endemic hotspot for the disease in Iran.

Abstract Background European epidemic intelligence (EI) systems receive vast amounts of information and data on disease outbreaks and potential health threats. The quantity and variety of available data sources for EI, as well as the available methods to manage and analyse these data sources, are constantly increasing. Our aim was to identify the difficulties encountered in this context and which innovations, according to EI practitioners, could improve the detection, monitoring and analysis of disease outbreaks and the emergence of new pathogens. Methods We conducted a qualitative study to identify the need for innovation expressed by 33 EI practitioners of national public health and animal health agencies in five European countries and at the European Centre for Disease Prevention and Control (ECDC). We adopted a stepwise approach to identify the EI stakeholders, to understand the problems they faced concerning their EI activities, and to validate and further define with practitioners the problems to address and the most adapted solutions to their work conditions. We characterized their EI activities, professional logics, and desired changes in their activities using NvivoⓇ software. Results Our analysis highlights that EI practitioners wished to collectively review their EI strategy to enhance their preparedness for emerging infectious diseases, adapt their routines to manage an increasing amount of data and have methodological support for cross-sectoral analysis. Practitioners were in demand of timely, validated and standardized data acquisition processes by text mining of various sources; better validated dataflows respecting the data protection rules; and more interoperable data with homogeneous quality levels and standardized covariate sets for epidemiological assessments of national EI. The set of solutions identified to facilitate risk detection and risk assessment included visualization, text mining, and predefined analytical tools combined with methodological guidance. Practitioners also highlighted their preference for partial rather than full automation of analyses to maintain control over the data and inputs and to adapt parameters to versatile objectives and characteristics. Conclusions The study showed that the set of solutions needed by practitioners had to be based on holistic and integrated approaches for monitoring zoonosis and antimicrobial resistance and on harmonization between agencies and sectors while maintaining flexibility in the choice of tools and methods. The technical requirements should be defined in detail by iterative exchanges with EI practitioners and decision-makers.

Background: Traditional medicine (TM) interventions are plausible therapeutic alternatives to conventional medical interventions against emerging and endemic zoonotic diseases, particularly in low-and middle-income countries that may lack resources and infrastructure. Despite the growing popularity in the usage of TM interventions, their clinical safety and effectiveness are still contested within conventional healthcare in many countries. Methods: We conducted a scoping review of the peer-reviewed literature that synthesises and maps the evidence on TM interventions for the treatment and prevention of zoonoses on the Indian subcontinent. The region, a global hotspot of biodiversity and emerging infections, is characterised by high prevalence of TM use. Based on the scientific literature (mostly case study research, n=l06 studies), our review (1) maps the scope of the literature, (2) synthesises the evidence on the application of TM interventions for zoonoses, and (3) critically reflects on the state of TM and identifies areas for future research focus. Results: The evidence synthesis confirmed widespread usage of TM interventions for zoonoses on the subcontinent, with the majority of research reported from India (n=99 studies, 93.4%), followed by Pakistan (n=3 studies, 2.8%), Bangladesh (n=2 studies, 1.9%), and Sri Lanka (n=1, 0.9%). Most of the reviewed studies reported on ethno-medicinal uses of plant species, primarily for treating dengue (n=20 studies), tuberculosis (n=18 studies), Escherichia coli infection (n=16 studies), lymphatic filariasis and cholera (n=9 apiece). However, the evidence on the safety and effectiveness of these reported TM interventions is limited, indicating that these data are rarely collected and/or shared within the peer-reviewed literature. Conclusion: This review thus highlights that, whilst TMs are already being used and could offer more widely accessible interventions against emerging and endemic zoonoses and ectoparasites, there is an urgent need for rigorous clinical testing and validation of the safety and effectiveness of these interventions. [ABSTRACT FROM AUTHOR]

Canine health is consistently affected by the circulation of the H3N2 strain of canine influenza virus (CIV). Prior research has indicated that the isolation rate of H3N2 CIVs in dogs has gradually increased in China, and these viruses have progressively adapted to humans over the course of their evolution within canine hosts, posing a significant public health threat. However, the key factors influencing the spread of CIVs remain elusive. From January 2020 to December 2022, during the COVID-19 pandemic, strict epidemic prevention policies were implemented in China. Thus, this measure provides an ideal model for studying factors influencing the prevalence of CIVs. In this study, we continuously monitored the prevalence of CIVs in China before and after the COVID-19 pandemic. We found that the pathogen detection rate and seropositivity rate of domestic CIVs significantly declined after the implementation of epidemic control measures. However, after restrictions on human movement were lifted in 2023, the circulation of CIVs gradually increased. Our results demonstrate that restricting human activity directly impacts the epidemic caused by CIVs and provide a theoretical basis for the implementation of control measures during outbreaks of zoonotic diseases in human companion animals. [ABSTRACT FROM AUTHOR]