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• A systematic solution for full-field 3D motion and deformation of high speed rotation object is presented, which combines high-speed rotation image acquisition using low-speed camera with 3D digital image correlation, both low-cost, flexible and accurate. • The process of phase-locked signal generation is explained, which is used to trigger the camera in a "phase-stepping" way so as to allow low-speed camera to achieve the entire circle images acquisition of high-speed rotation asynchronously. • A layer-by-layer matching method based on closest point is proposed to search uncoded targets between two rotated images, futherly the 3D motion parameters can be obtained after 3D reconstruction and singular value decomposition. • A binocular rotation correlating strategy is proposed to correlate the speckle subsets between a referece image and a rotated image, which is accurate and robust for the decorrelation problem in DIC. • In addition to the 3D motion and deformation measurement of high-speed rotating objects, our method can also be used for full-field 3D deformation measurement of high-speed vibration, and online fault diagnosis under various cyclic alternating load conditions, etc. The full-field and non-contact measurement of three-dimensional motion and deformation of high-speed rotating machinery is a very challenging task in the engineering field. Two issues, the cost of high-speed camera and the decorrelation between rotated images, are hard to avoid for investigators. In this paper, a systematic solution is presented, which combines high-speed rotation image acquisition using low-speed camera with 3D digital image correlation, both low-cost, flexible and accurate. Firstly, the frequency and initial phase of high-speed rotation are calculated by FFT, and temporal phase-locked signal is generated to trigger the dual-camera asynchronously to collect the circumferential images with equal phase interval. Then a layer-by-layer matching method is proposed to search uncoded targets between two rotated images, while a binocular rotation correlating strategy to correlate the speckle subsets. Finally, the corresponded uncoded targets and speckle subsets are reconstructed in three dimension via the camera calibration results, furtherly the 3D motion and deformation fields can be obtained. Five experiments, including two measurement repeatability tests, an accuracy test, a rigid motion measurement of rotating table and a dynamic deformation measurement of ventilation fan, prove the accuracy and effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

Objectives: Aminoglycoside-induced cochlear ototoxicity causes inner ear hair cells (HCs) loss and leads to hearing impairment in patients, but no treatment completely eliminates the ototoxic effect. This study aims to determine the effectiveness of (-)-Epigallocatechin-3-gallate (EGCG) as a protective agent against aminoglycoside-induced ototoxicity., Methods: Zebrafish were exposed to EGCG for 24 h and then co-treated with EGCG and ototoxic agent (amikacin and gentamicin) for 5 h to explore the protective effect of EGCG on zebrafish HCs. Network pharmacology analysis and molecular docking simulation were conducted to explore its potential mechanism, and in vitro cell experiments were used to validate the outcome of the result., Result: EGCG against ototoxicity of aminoglycosides in zebrafish HCs. Network pharmacology analysis and molecular docking showing that molecules related to cellular response regulation to oxidative stress, including AKT1, DHFR, and MET, may be the target proteins of EGCG, which were verified in vitro experiments. Further experiments demonstrated thatEGCG can antagonize the death of HCs caused by amikacin and gentamicin by reducing intracellular reactive oxygen species (ROS) accumulation and anti-apoptosis., Conclusion: EGCG can be an otoprotective drug against aminoglycosides-induced ototoxicity, prevent cellular apoptosis and significantly reduce oxidative stress., (Copyright © 2021 Elsevier B.V. All rights reserved.)