20 results on '"Zollo, I."'
Search Results
2. Designing the school-family alliance from an inclusive and sustainable perspective: an operational proposal
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Zollo, I. and Galdieri, M.
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Inclusive design ,school-family alliance ,accessibility ,sustainability ,teacher education - Published
- 2022
3. Corrigendum: Age-Related Differences in the Expression of Most Relevant Mediators of SARS-CoV-2 Infection in Human Respiratory and Gastrointestinal Tract (Front. Pediatr., (2021), 9, (697390), 10.3389/fped.2021.697390)
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Berni Canani R., Comegna M., Paparo L., Cernera G., Bruno C., Strisciuglio C., Zollo I., Gravina A. G., Miele E., Cantone E., Gennarelli N., Nocerino R., Carucci L., Giglio V., Amato F., Castaldo G., Berni Canani, R., Comegna, M., Paparo, L., Cernera, G., Bruno, C., Strisciuglio, C., Zollo, I., Gravina, A. G., Miele, E., Cantone, E., Gennarelli, N., Nocerino, R., Carucci, L., Giglio, V., Amato, F., and Castaldo, G.
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neuropilin-1 ,angiotensin-converting enzyme 2 ,transmembrane serine protease-2 ,COVID-19 ,healthy subject - Abstract
In the original article, there was a mistake in the order of Figures 1 and 2 as published. The figures are given in the correct order below. The authors apologize for the error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
- Published
- 2021
4. 305 Development of an adenine base editing-based strategy to allow temporospatial control of correction of W1282X-CFTR.
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Zollo, I., Santos, L., Alves, J., Harrison, P., and Farinha, C.
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- *
ADENINE - Published
- 2024
- Full Text
- View/download PDF
5. L'agire corporeo
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Sibilio, M., Di Gennaro, D. C., and Zollo, I.
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- 2017
6. Didattica semplessa e competenze di lettura nella scuola primaria
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Di Tore, S. and Zollo, I.
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- 2016
7. Age-related differences in the expression of most relevant mediators of SARS-coV-2 infection in the respiratory and gastrointestinal tract
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Canani, Berni R., Comegna, M., Paparo, L., Cernera, G., Bruno, C., Strisciuglio, C., Zollo, I., Gravina, A., Miele, E., Cantone, E., Gennarelli, N., RITA NOCERINO, Carucci, L., Giglio, V., Amato, F., and Castaldo, G.
8. Age-Related Differences in the Expression of Most Relevant Mediators of SARS-CoV-2 Infection in Human Respiratory and Gastrointestinal Tract
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Roberto Berni Canani, Marika Comegna, Lorella Paparo, Gustavo Cernera, Cristina Bruno, Caterina Strisciuglio, Immacolata Zollo, Antonietta Gerarda Gravina, Erasmo Miele, Elena Cantone, Nicola Gennarelli, Rita Nocerino, Laura Carucci, Veronica Giglio, Felice Amato, Giuseppe Castaldo, Berni Canani, R., Comegna, M., Paparo, L., Cernera, G., Bruno, C., Strisciuglio, C., Zollo, I., Gravina, A. G., Miele, E., Cantone, E., Gennarelli, N., Nocerino, R., Carucci, L., Giglio, V., Amato, F., Castaldo, G., Berni Canani, Roberto, Comegna, Marika, Paparo, Lorella, Cernera, Gustavo, Bruno, Cristina, Strisciuglio, Caterina, Zollo, Immacolata, Gravina, Antonietta Gerarda, Miele, Erasmo, Cantone, Elena, Gennarelli, Nicola, Nocerino, Rita, Carucci, Laura, Giglio, Veronica, Amato, Felice, and Castaldo, Giuseppe
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0301 basic medicine ,transmembrane serine protease-2 ,healthy subject ,Pediatrics ,TMPRSS2 ,RJ1-570 ,Virus ,03 medical and health sciences ,0302 clinical medicine ,angiotensin-converting enzyme 2 ,Medicine ,030212 general & internal medicine ,Respiratory system ,Gastrointestinal tract ,business.industry ,Correction ,COVID-19 ,Brief Research Report ,Small intestine ,neuropilin-1 ,030104 developmental biology ,medicine.anatomical_structure ,Expression (architecture) ,healthy subjects ,Pediatrics, Perinatology and Child Health ,Immunology ,Angiotensin-converting enzyme 2 ,business ,Respiratory tract - Abstract
Background: Clinical features of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection seem to differ in children compared to that in adults. It has been hypothesized that the lower clinical severity in children could be influenced by differential expression of the main host functional receptor to SARS-CoV-2, the angiotensin-converting enzyme 2 (ACE2), but data are still conflicting. To explore the origin of age-dependent clinical features of coronavirus disease 2019 (COVID-19), we comparatively evaluated the expression in children and adult subjects of the most relevant mediators of the SARS-CoV-2 infection: ACE2, angiotensin-converting enzyme 1 (ACE1), transmembrane serine protease-2 (TMPRSS2), and neuropilin-1 (NRP1), at upper respiratory tract and small intestine level.Methods: The expression of ACE2, ACE1, TMPRSS2, and NRP1 in nasal epithelium and in small intestine epithelium was investigated by quantitative real-time PCR analysis.Results: We found no differences in ACE2, ACE1, and TMPRSS2 expression in the nasal epithelium comparing children and adult subjects. In contrast, nasal epithelium NRP1 expression was lower in children compared to that in adults. Intestinal ACE2 expression was higher in children compared to that in adults, whereas intestinal ACE1 expression was higher in adults. Intestinal TMPRSS2 and NRP1 expression was similar comparing children and adult subjects.Conclusions: The lower severity of SARS-CoV-2 infection observed in children may be due to a different expression of nasal NRP1, that promotes the virus interaction with ACE2. However, the common findings of intestinal symptoms in children could be due to a higher expression of ACE2 at this level. The insights from these data will be useful in determining the treatment policies and preventive measures for COVID-19.
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- 2021
9. Elexacaftor–Tezacaftor–Ivacaftor Therapy for Cystic Fibrosis Patients with The F508del/Unknown Genotype
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Antonella Miriam Di Lullo, Giuseppe Castaldo, Carmela Colangelo, Immacolata Zollo, Donatello Salvatore, Vito Terlizzi, Giovanni Taccetti, Marika Comegna, Felice Amato, Comegna, M., Terlizzi, V., Salvatore, D., Colangelo, C., Di Lullo, A. M., Zollo, I., Taccetti, G., Castaldo, G., and Amato, F.
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0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,RM1-950 ,Biochemistry ,Microbiology ,Cystic fibrosis ,Gastroenterology ,Article ,Ivacaftor ,cystic fibrosis ,03 medical and health sciences ,theratyping ,0302 clinical medicine ,Internal medicine ,Genotype ,medicine ,biochemistry ,Pharmacology (medical) ,Functional studies ,General Pharmacology, Toxicology and Pharmaceutics ,Allele ,CFTR ,rare mutation ,business.industry ,functional characterization ,personalized medicine ,medicine.disease ,030104 developmental biology ,Infectious Diseases ,030228 respiratory system ,Cystic fibrosi ,Mutation (genetic algorithm) ,Tezacaftor ,Personalized medicine ,Therapeutics. Pharmacology ,business ,medicine.drug - Abstract
The new CFTR modulator combination, elexacaftor/tezacaftor/ivacaftor (Trikafta) was approved by the FDA in October 2019 for treatment of Cystic Fibrosis in patients 6 years of age or older who have at least one F508del mutation in one allele and a minimal-function or another F508del mutation in the other allele. However, there is a group of patients, in addition to those with rare mutations, in which despite the presence of a F508del in one allele, it was not possible to identify any mutation in the other allele. To date, these patients are excluded from treatment with Trikafta in Italy, where the CF patients carrying F508del/unknown represent about 1.3% (71 patients) of the overall Italian CF patients. In this paper we show that the Trikafta treatment of nasal epithelial cells, derived from F508del/Unknown patients, results in a significant rescue of CFTR activity. Based on our findings, we think that the F508del/Unknown patients considered in this study could obtain clinical benefits from Trikafta treatment, and we strongly suggest their eligibility for this type of treatment. This study, adding further evidence in the literature, once again confirms the validity of functional studies on nasal cells in the cystic fibrosis theratyping and personalized medicine.
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- 2021
10. La progettazione del sé professionale alla fine dell’adolescenza
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Di Vita, A, Zanniello, G, Lucisano, P, Zanniello, G, Giuliani, A, Sposetti, P, Szpunar, G, Fioretti, F, Di Vita, A, De Santis, M, Crispoldi, S, Biasi, V, Caggiano, V, Ciraci, AM, Moretti, G, Morini, AL, La Rocca, C, Casale, E, Zollo, I, Sibilio, M, Nuzzaci, A, Marcozzi, I, Calenda, M, Ferrantino, C, Petolicchio, A, Milito, F, Rubat du Mérac, E, De Santis, C, Marco De Luca, A, Tammaro, R, Tiso, M, Montalbetti, K, Orizio, E, Kasa, P, de Luca, AM, Vegliante, R, Miranda, S, De Angelis, M, Santonicola, M, Marzano, A, Lisimberti, C, Rossi, L, Leone, T, Salerni, A, Castellana, G, Margottini, M, Rossi, F, De Carlo, F, Poce, A, Amenduni, F, Re, MR, La Marca, A, Di Martino, V, Gülbay, E, Mitrovic, S, Silva, L, Batini, F, Bartolucci, M, Maccario, D, Menichetti, L, Cavicchioli, C, and Consoli, G
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strategia di orientamento ,progetto di vita professionale ,competenze trasversali ,guidance strategy ,transversal skills ,high school-university ,professional life project ,liceo-università ,Settore M-PED/03 - Didattica E Pedagogia Speciale - Abstract
Si è conclusa il 15 maggio 2019 la prima parte di una ricerca- intervento ispirata alla teoria del pensiero narrativo, ai principi dell’educational counseling e della didattica orientativa. Duran- te l’anno scolastico 2018-2019, 93 studenti (50 femmine e 43 maschi) del quarto anno di cinque licei di Palermo hanno partecipato a un’iniziativa sperimentale in cui scuola e università hanno collaborato con un duplice scopo: ridurre negli studenti il gap rilevato tra le conoscenze in uscita dal liceo e quelle richieste all’inizio dei corsi universitari e potenziare in loro la capacità di formulare il proprio progetto di vita professionale. È stata sperimentata l’azione congiunta di tre tipi di interventi: la scrittura espressiva per prefigurare il proprio futuro formativo-professionale, il colloquio di orientamento per esercitare la propria capacità decisionale e la co-conduzione di laboratori pomeridiani da parte di un docente universitario e di un docente di liceo della stessa area disciplinare. Si è voluto verificare fino a che punto la partecipazione all’iniziativa facilitasse lo sviluppo negli studenti liceali di tre competenze trasversali che incidono significativamente nel processo di maturazione della scelta formativo-professionale: l’immagine adeguata del «sé» attuale, l’autoefficacia percepita e la capacità decisionale. La valutazione iniziale del grado di sviluppo delle tre competenze che si intendeva promuovere è stata effettuata nel novembre del 2018 e quella finale nel mese di maggio del 2019; inoltre, una valutazione intermedia dell’immagine del «sé» attuale è stata effettuata anche a gennaio e a marzo del 2019. Per verificare la significatività delle differenze «pre-post» è stato usato il test statistico t. Le differenze risultano significative per il costrutto dell’autoefficacia (p=0,004) e per la dimensione «energia» (p=0,0002) dell’immagine del «sé» attuale. Dopo lo svolgimento delle attività gli alunni si percepiscono più capaci di affrontare i compiti scolastici. The first part of an intervention-research, inspired on the the- ory of narrative thought, the principles of educational coun- seling and guidance in teaching, was concluded on May 15th 2019. During the school year 2018-2019, students (n=93, 50 females and 43 males) attending the fourth year of high school in Palermo, participated in an experimental initiative in which schools and university collaborated with a dual purpose: 1) to reduce in the students the gap between the acquired knowl- edges at the end of high school courses and those required at the beginning of university courses and, 2) to strengthen the ability to formulate their own professional life project. The joint action of three types of interventions was experimented: expressive writing to prefigure one’s own educational-profes- sional future, the guidance interview to exercise one’s decision- making skill and the conduction of afternoon teaching work- shops held by a university professor and a high school teacher from the same discipline. It was intended to verify if the par- ticipation in this initiative would facilitate, in high school stu- dents, the development of three transversal skills that signifi- cantly affect the process of maturation of the educational-pro- fessional choice: the adequate image of the current «oneself», the perceived self-efficacy and the decision-making skill. The initial assessment of the development degree of the three skills that were intended to promote, was carried out firstly, in November 2018 and lastly but not least, in May 2019. More- over, an intermediate assessment of the current «oneself» im- age was carried out in January and March 2019. The statistical t-test was used to verify the significance of the «pre-post» dif- ferences. There is, not only a significant difference in the con- struct of self-efficacy (p = 0.004) but also in the «energy» di- mension of the current «oneself» image (p = 0.0002). After carrying out the activities, the students perceive themselves as more able of dealing with school tasks.
- Published
- 2020
11. Tipologie di ricerca didattica in Italia (2016-2018)
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Giuseppe Zanniello, Lucisano, P, Zanniello, G, Giuliani, A, Sposetti, P, Szpunar, G, Fioretti, F, Di Vita, A, De Santis, M, Crispoldi, S, Biasi, V, Caggiano, V, Ciraci, AM, Moretti, G, Morini, AL, La Rocca, C, Casale, E, Zollo, I, Sibilio, M, Nuzzaci, A, Marcozzi, I, Calenda, M, Ferrantino, C, Petolicchio, A, Milito, F, Rubat du Mérac, E, De Santis, C, Marco De Luca, A, Tammaro, R, Tiso, M, Montalbetti, K, Orizio, E, Kasa, P, de Luca, AM, Vegliante, R, Miranda, S, De Angelis, M, Santonicola, M, Marzano, A, Lisimberti, C, Rossi, L, Leone, T, Salerni, A, Castellana, G, Margottini, M, Rossi, F, De Carlo, F, Poce, A, Amenduni, F, Re, MR, La Marca, A, Di Martino, V, Gülbay, E, Mitrovic, S, Silva, L, Batini, F, Bartolucci, M, Maccario, D, Menichetti, L, Cavicchioli, C, Consoli, G, and Giuseppe Zanniello
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teaching research methods, teaching research topics, perspectives of didactic research ,metodi della ricerca didattica, temi della ricerca didattica, prospettive della ricerca didattica ,Settore M-PED/03 - Didattica E Pedagogia Speciale - Abstract
In Italia, nel triennio 2016-2018, si è fatta ricerca didattica sul processo di insegnamento- apprendimento in tre modi: l’analisi fenomenologica e l’osservazione della dimensione quantitativa della realtà educativa; la costruzione di categorie interpretative del collegamento delle azioni dei docenti con i cambiamenti osservati nei discenti; la formulazione di principi e orientamenti per la pratica didattica. I temi delle ricerche didattiche svolte nel periodo sono raggruppabili in quattordici aree: la didattica universitaria, la didattica inclusiva, la didattica interculturale, la didattica orientativa, la didattica metacognitiva, la raccolta e l’analisi di pratiche didattiche, le nuove tecnologie per l’insegnamento e l’apprendimento, la valutazione degli alunni, la valutazione degli insegnanti e degli istituti scolastici, la didattica personalizzata, la progettazione degli interventi formativi, la formazione iniziale degli insegnanti, i servizi educativi per l’infanzia, le didattiche delle discipline. Per la cumulabilità dei risultati delle ricerche didattiche è avvertita l’esigenza di procedure metodologiche condivise dall’intera comunità scientifica. In the 2016-2018 three-year period, the Italian teaching research was carried out on the teaching- learning process in three ways: the phenomenological analysis and the observation of the quantitative dimension of the educational reality; the construction of categories to interpret the connections between teachers' actions and the changes observed in the learners; the formulation of principles and guidelines for the teaching practice. The themes of the teaching research carried out in the period can be grouped into fourteen areas: university teaching, inclusive teaching, intercultural teaching, orientation teaching, metacognitive teaching, the collection and analysis of teaching practices, new technologies for teaching and learning, pupils' evaluation, teachers' and schools' evaluation, personalized teaching, planning of training interventions, initial teacher training, educational services for children, didactics of the disciplines. Because of the accumulation of the results of educational research, there is a need for sharing methodological procedures within the entire scientific community.
- Published
- 2020
12. Identification of an ultra-rare Alu insertion in the CFTR gene: Pitfalls and challenges in genetic test interpretation.
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Esposito S, Zollo I, Villella VR, Scialò F, Giordano S, Esposito MV, Salemme N, Di Domenico C, Cernera G, Zarrilli F, Castaldo G, and Amato F
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- Humans, Infant, Newborn, Male, Female, Cystic Fibrosis Transmembrane Conductance Regulator genetics, Alu Elements genetics, Cystic Fibrosis genetics, Cystic Fibrosis diagnosis, Genetic Testing methods
- Abstract
Cystic fibrosis (CF) is a life-limiting genetic disorder characterized by defective chloride ion transport due to mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Early detection through newborn screening programs significantly improves outcomes for individuals with CF by enabling timely intervention. Here, we report the identification of an Alu element insertion within the exon 15 of CFTR gene, initially overlooked in standard next-generation sequencing analyses. However, using traditional molecular techniques, based on polymerase chain reaction and Sanger sequencing, allowed the identification of the Alu element and the reporting of a correct diagnosis. Our analysis, based on bioinformatics tools and molecular techniques, revealed that the Alu element insertion severely affects the gene expression, splicing patterns, and structure of CFTR protein. In conclusion, this study emphasizes the importance of how the integration of human expertise and modern technologies represents a pivotal step forward in genomic medicine, ensuring the delivery of precision healthcare to individuals affected by genetic diseases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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13. In silico analysis and theratyping of an ultra-rare CFTR genotype (W57G/A234D) in primary human rectal and nasal epithelial cells.
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Kleinfelder K, Lotti V, Eramo A, Amato F, Lo Cicero S, Castelli G, Spadaro F, Farinazzo A, Dell'Orco D, Preato S, Conti J, Rodella L, Tomba F, Cerofolini A, Baldisseri E, Bertini M, Volpi S, Villella VR, Esposito S, Zollo I, Castaldo G, Laudanna C, Sorsher EJ, Hong J, Joshi D, Cutting G, Lucarelli M, Melotti P, and Sorio C
- Abstract
Mutation targeted therapy in cystic fibrosis (CF) is still not eligible for all CF subjects, especially for cases carrying rare variants such as the CFTR genotype W57G/A234D (c.169T>G/c.701C>A). We performed in silico analysis of the effects of these variants on protein stability, which we functionally characterized using colonoids and reprogrammed nasal epithelial cells. The effect of mutations on cystic fibrosis transmembrane conductance regulator (CFTR) protein was analyzed by western blotting, forskolin-induced swelling (FIS), and Ussing chamber analysis. We detected a residual CFTR function that increases following treatment with the CFTR modulators VX661±VX445±VX770, correlates among models, and is associated with increased CFTR protein levels following treatment with CFTR correctors. In vivo treatment with VX770 reduced sweat chloride concentration to non-CF levels, increased the number of CFTR-dependent sweat droplets, and induced a 6% absolute increase in predicted FEV1% after 27 weeks of treatment indicating the relevance of theratyping with patient-derived cells in CF., Competing Interests: P.M. was paid for her expert testimony by Vertex Pharmaceuticals., (© 2023 The Author(s).)
- Published
- 2023
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14. Correction to: L1077P CFTR pathogenic variant function rescue by Elexacaftor-Tezacaftor-Ivacaftor in cystic fbrosis patient-derived air-liquid interface (ALI) cultures and organoids: in vitro guided personalized therapy of non-F508del patients.
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Lo Cicero S, Castelli G, Blaconà G, Bruno SM, Sette G, Pigliucci R, Villella VR, Esposito S, Zollo I, Spadaro F, De Maria R, Biffoni M, Cimino G, Amato F, Lucarelli M, and Eramo A
- Published
- 2023
- Full Text
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15. L1077P CFTR pathogenic variant function rescue by Elexacaftor-Tezacaftor-Ivacaftor in cystic fibrosis patient-derived air-liquid interface (ALI) cultures and organoids: in vitro guided personalized therapy of non-F508del patients.
- Author
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Lo Cicero S, Castelli G, Blaconà G, Bruno SM, Sette G, Pigliucci R, Villella VR, Esposito S, Zollo I, Spadaro F, Maria R, Biffoni M, Cimino G, Amato F, Lucarelli M, and Eramo A
- Subjects
- Humans, Cystic Fibrosis Transmembrane Conductance Regulator genetics, Cystic Fibrosis drug therapy, Cystic Fibrosis genetics
- Abstract
Cystic fibrosis (CF) is caused by defects of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CFTR-modulating drugs may overcome specific defects, such as the case of Trikafta, which is a clinically approved triple combination of Elexacaftor, Tezacaftor and Ivacaftor (ETI) that exhibited a strong ability to rescue the function of the most frequent F508del pathogenic variant even in genotypes with the mutated allele in single copy. Nevertheless, most rare genotypes lacking the F508del allele are still not eligible for targeted therapies. Via the innovative approach of using nasal conditionally reprogrammed cell (CRC) cell-based models that mimic patient disease in vitro, which are obtainable from each patient due to the 100% efficiency of the cell culture establishment, we theratyped orphan CFTR mutation L1077P. Protein studies, Forskolin-induced organoid swelling, and Ussing chamber assays congruently proved the L1077P variant function rescue by ETI. Notably, this rescue takes place even in the context of a single-copy L1077P allele, which appears to enhance its expression. Thus, the possibility of single-allele treatment also arises for rare genotypes, with an allele-specific modulation as part of the mechanism. Of note, besides providing indication of drug efficacy with respect to specific CFTR pathogenic variants or genotypes, this approach allows the evaluation of the response of single-patient cells within their genetic background. In this view, our studies support in vitro guided personalized CF therapies also for rare patients who are nearly excluded from clinical trials., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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16. Comparative Analysis of a Human Neutralizing mAb Specific for SARS-CoV-2 Spike-RBD with Cilgavimab and Tixagevimab for the Efficacy on the Omicron Variant in Neutralizing and Detection Assays.
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Passariello M, Esposito S, Manna L, Rapuano Lembo R, Zollo I, Sasso E, Amato F, and De Lorenzo C
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- Humans, Antibodies, Monoclonal therapeutic use, Antibodies, Neutralizing, Antibodies, Viral therapeutic use, SARS-CoV-2 genetics, COVID-19
- Abstract
The recent pandemic years have prompted the scientific community to increasingly search for and adopt new and more efficient therapeutic and diagnostic approaches to deal with a new infection. In addition to the development of vaccines, which has played a leading role in fighting the pandemic, the development of monoclonal antibodies has also represented a valid approach in the prevention and treatment of many cases of CoronaVirus Disease 2019 (COVID-19). Recently, we reported the development of a human antibody, named D3, showing neutralizing activity against different SARS-CoV-2 variants, wild-type, UK, Delta and Gamma variants. Here, we have further characterized with different methods D3's ability to bind the Omicron-derived recombinant RBD by comparing it with the antibodies Cilgavimab and Tixagevimab, recently approved for prophylactic use of COVID-19. We demonstrate here that D3 binds to a distinct epitope from that recognized by Cilgavimab and shows a different binding kinetic behavior. Furthermore, we report that the ability of D3 to bind the recombinant Omicron RBD domain in vitro results in a good ability to also neutralize Omicron-pseudotyped virus infection in ACE2-expressing cell cultures. We point out here that D3 mAb maintains a good ability to recognize both the wild-type and Omicron Spike proteins, either when used as recombinant purified proteins or when expressed on pseudoviral particles despite the different variants, making it particularly useful both from a therapeutic and diagnostic point of view. On the basis of these results, we propose to exploit this mAb for combinatorial treatments with other neutralizing mAbs to increase their therapeutic efficacy and for diagnostic use to measure the viral load in biological samples in the current and future pandemic waves of coronaviruses.
- Published
- 2023
- Full Text
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17. The Immune Response to SARS-CoV-2 Vaccine in a Cohort of Family Pediatricians from Southern Italy.
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Cortese P, Amato F, Davino A, De Franchis R, Esposito S, Zollo I, Di Domenico M, Solito E, Zarrilli F, Gentile L, Cernera G, and Castaldo G
- Subjects
- Humans, BNT162 Vaccine, Leukocytes, Mononuclear, Tumor Necrosis Factor-alpha, SARS-CoV-2, Pediatricians, Italy, Immunity, COVID-19 Vaccines, COVID-19 prevention & control
- Abstract
In Italy, from January 2021, the Ministry of Health indicated a vaccination plan against COVID for frail patients and physicians based on a three-dose scheme. However, conflicting results have been reported on which biomarkers permit immunization assessment. We used several laboratory approaches (i.e., antibodies serum levels, flow cytometry analysis, and cytokines release by stimulated cells) to investigate the immune response in a cohort of 53 family pediatricians (FPs) at different times after the vaccine. We observed that the BNT162b2-mRNA vaccine induced a significant increase of specific antibodies after the third (booster) dose; however, the antibody titer was not predictive of the risk of developing the infection in the six months following the booster dose. The antigen stimulation of PBMC cells from subjects vaccinated with the third booster jab induced the increase of the activated T cells (i.e., CD4
+ CD154+ ); the frequency of CD4+ CD154+ TNF-α+ cells, as well as the TNF-α secretion, was not modified, while we observed a trend of increase of IFN-γ secretion. Interestingly, the level of CD8+ IFN-γ+ (independently from antibody titer) was significantly increased after the third dose and predicts the risk of developing the infection in the six months following the booster jab. Such results may impact also other virus vaccinations.- Published
- 2023
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18. Corrigendum: Age-Related Differences in the Expression of Most Relevant Mediators of SARS-CoV-2 Infection in Human Respiratory and Gastrointestinal Tract.
- Author
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Berni Canani R, Comegna M, Paparo L, Cernera G, Bruno C, Strisciuglio C, Zollo I, Gravina AG, Miele E, Cantone E, Gennarelli N, Nocerino R, Carucci L, Giglio V, Amato F, and Castaldo G
- Abstract
[This corrects the article DOI: 10.3389/fped.2021.697390.]., (Copyright © 2021 Berni Canani, Comegna, Paparo, Cernera, Bruno, Strisciuglio, Zollo, Gravina, Miele, Cantone, Gennarelli, Nocerino, Carucci, Giglio, Amato and Castaldo.)
- Published
- 2021
- Full Text
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19. Age-Related Differences in the Expression of Most Relevant Mediators of SARS-CoV-2 Infection in Human Respiratory and Gastrointestinal Tract.
- Author
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Berni Canani R, Comegna M, Paparo L, Cernera G, Bruno C, Strisciuglio C, Zollo I, Gravina AG, Miele E, Cantone E, Gennarelli N, Nocerino R, Carucci L, Giglio V, Amato F, and Castaldo G
- Abstract
Background: Clinical features of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection seem to differ in children compared to that in adults. It has been hypothesized that the lower clinical severity in children could be influenced by differential expression of the main host functional receptor to SARS-CoV-2, the angiotensin-converting enzyme 2 (ACE2), but data are still conflicting. To explore the origin of age-dependent clinical features of coronavirus disease 2019 (COVID-19), we comparatively evaluated the expression in children and adult subjects of the most relevant mediators of the SARS-CoV-2 infection: ACE2, angiotensin-converting enzyme 1 (ACE1), transmembrane serine protease-2 (TMPRSS2), and neuropilin-1 (NRP1), at upper respiratory tract and small intestine level. Methods: The expression of ACE2, ACE1, TMPRSS2 , and NRP1 in nasal epithelium and in small intestine epithelium was investigated by quantitative real-time PCR analysis. Results: We found no differences in ACE2, ACE1 , and TMPRSS2 expression in the nasal epithelium comparing children and adult subjects. In contrast, nasal epithelium NRP1 expression was lower in children compared to that in adults. Intestinal ACE2 expression was higher in children compared to that in adults, whereas intestinal ACE1 expression was higher in adults. Intestinal TMPRSS2 and NRP1 expression was similar comparing children and adult subjects. Conclusions: The lower severity of SARS-CoV-2 infection observed in children may be due to a different expression of nasal NRP1 , that promotes the virus interaction with ACE2. However, the common findings of intestinal symptoms in children could be due to a higher expression of ACE2 at this level. The insights from these data will be useful in determining the treatment policies and preventive measures for COVID-19., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Berni Canani, Comegna, Paparo, Cernera, Bruno, Strisciuglio, Zollo, Gravina, Miele, Cantone, Gennarelli, Nocerino, Carucci, Giglio, Amato and Castaldo.)
- Published
- 2021
- Full Text
- View/download PDF
20. Elexacaftor-Tezacaftor-Ivacaftor Therapy for Cystic Fibrosis Patients with The F508del/Unknown Genotype.
- Author
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Comegna M, Terlizzi V, Salvatore D, Colangelo C, Di Lullo AM, Zollo I, Taccetti G, Castaldo G, and Amato F
- Abstract
The new CFTR modulator combination, elexacaftor/tezacaftor/ivacaftor (Trikafta) was approved by the FDA in October 2019 for treatment of Cystic Fibrosis in patients 6 years of age or older who have at least one F508del mutation in one allele and a minimal-function or another F508del mutation in the other allele. However, there is a group of patients, in addition to those with rare mutations, in which despite the presence of a F508del in one allele, it was not possible to identify any mutation in the other allele. To date, these patients are excluded from treatment with Trikafta in Italy, where the CF patients carrying F508del/unknown represent about 1.3% (71 patients) of the overall Italian CF patients. In this paper we show that the Trikafta treatment of nasal epithelial cells, derived from F508del/Unknown patients, results in a significant rescue of CFTR activity. Based on our findings, we think that the F508del/Unknown patients considered in this study could obtain clinical benefits from Trikafta treatment, and we strongly suggest their eligibility for this type of treatment. This study, adding further evidence in the literature, once again confirms the validity of functional studies on nasal cells in the cystic fibrosis theratyping and personalized medicine.
- Published
- 2021
- Full Text
- View/download PDF
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