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129 results on '"Zoia, Chiara Paola"'

1. Harmonization of sensorimotor deficit assessment in a registered multicentre pre-clinical randomized controlled trial using two models of ischemic stroke

Author

Valente, Alessia, Mariani, Jacopo, Seminara, Serena, Tettamanti, Mauro, Pignataro, Giuseppe, Perego, Carlo, Sironi, Luigi, Pedata, Felicita, Amantea, Diana, Bacigaluppi, Marco, Vinciguerra, Antonio, Diamanti, Susanna, Viganò, Martina, Santangelo, Francesco, Zoia, Chiara Paola, Rodriguez-Menendez, Virginia, Castiglioni, Laura, Rzemieniec, Joanna, Dettori, Ilaria, Bulli, Irene, Coppi, Elisabetta, Di Santo, Chiara, Cuomo, Ornella, Gullotta, Giorgia Serena, Butti, Erica, Bagetta, Giacinto, Martino, Gianvito, De Simoni, Maria-Grazia, Ferrarese, Carlo, Fumagalli, Stefano, and Beretta, Simone

Published
2023
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2. Glibenclamide-Loaded Engineered Nanovectors (GNVs) Modulate Autophagy and NLRP3-Inflammasome Activation

Author

Saresella, Marina, primary, Zoia, Chiara Paola, additional, La Rosa, Francesca, additional, Bazzini, Chiara, additional, Sala, Gessica, additional, Grassenis, Erica, additional, Marventano, Ivana, additional, Hernis, Ambra, additional, Piancone, Federica, additional, Conti, Elisa, additional, Sesana, Silvia, additional, Re, Francesca, additional, Seneci, Pierfausto, additional, Ferrarese, Carlo, additional, and Clerici, Mario, additional

Published
2023
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5. Author Correction: Common variants in Alzheimer’s disease and risk stratification by polygenic risk scores

Author

de Rojas, Itziar, Moreno-Grau, Sonia, Tesi, Niccolo, Grenier-Boley, Benjamin, Andrade, Victor, Jansen, Iris E., Pedersen, Nancy L., Stringa, Najada, Zettergren, Anna, Hernández, Isabel, Antúnez, Carmen, Antonell, Anna, Tankard, Rick M., Bis, Joshua C., Sims, Rebecca, Bellenguez, Céline, Quintela, Inés, González-Perez, Antonio, Calero, Miguel, Macías, Juan, Blesa, Rafael, Cervera-Carles, Laura, Menéndez-González, Manuel, Royo, Jose Luís, Moreno, Fermin, Huerto Vilas, Raquel, Baquero, Miquel, Diez-Fairen, Mónica, Lage, Carmen, García-González, Pablo, Valero, Sergi, Ullgren, Abbe, Naj, Adam C., Lemstra, Afina W., Benussi, Alberto, Rábano, Alberto, Padovani, Alessandro, Squassina, Alessio, de Mendonça, Alexandre, Arias Pastor, Alfonso, Kok, Almar A. L., Meggy, Alun, Pastor, Ana Belén, Espinosa, Ana, Corma-Gómez, Anaïs, Sanabria, Ángela, DeStefano, Anita L., Schneider, Anja, Haapasalo, Annakaisa, Kinhult Ståhlbom, Anne, Tybjærg-Hansen, Anne, Hartmann, Annette M., Spottke, Annika, Corbatón-Anchuelo, Arturo, Rongve, Arvid, Borroni, Barbara, Arosio, Beatrice, Nacmias, Benedetta, Nordestgaard, Børge G., Kunkle, Brian W., Charbonnier, Camille, Masullo, Carlo, Martínez Rodríguez, Carmen, Muñoz-Fernandez, Carmen, Dufouil, Carole, Graff, Caroline, Ferreira, Catarina B., Chillotti, Caterina, Reynolds, Chandra A., Fenoglio, Chiara, Van Broeckhoven, Christine, Clark, Christopher, Pisanu, Claudia, Satizabal, Claudia L., Holmes, Clive, Buiza-Rueda, Dolores, Aarsland, Dag, Rujescu, Dan, Alcolea, Daniel, Galimberti, Daniela, Wallon, David, Seripa, Davide, Grünblatt, Edna, Dardiotis, Efthimios, Düzel, Emrah, Scarpini, Elio, Conti, Elisa, Rubino, Elisa, Gelpi, Ellen, Rodriguez-Rodriguez, Eloy, Duron, Emmanuelle, Boerwinkle, Eric, Ferri, Evelyn, Tagliavini, Fabrizio, Küçükali, Fahri, Pasquier, Florence, Sanchez-Garcia, Florentino, Mangialasche, Francesca, Jessen, Frank, Nicolas, Gaël, Selbæk, Geir, Ortega, Gemma, Chêne, Geneviève, Hadjigeorgiou, Georgios, Rossi, Giacomina, Spalletta, Gianfranco, Giaccone, Giorgio, Grande, Giulia, Binetti, Giuliano, Papenberg, Goran, Hampel, Harald, Bailly, Henri, Zetterberg, Henrik, Soininen, Hilkka, Karlsson, Ida K., Alvarez, Ignacio, Appollonio, Ildebrando, Giegling, Ina, Skoog, Ingmar, Saltvedt, Ingvild, Rainero, Innocenzo, Rosas Allende, Irene, Hort, Jakub, Diehl-Schmid, Janine, Van Dongen, Jasper, Vidal, Jean-Sebastien, Lehtisalo, Jenni, Wiltfang, Jens, Thomassen, Jesper Qvist, Kornhuber, Johannes, Haines, Jonathan L., Vogelgsang, Jonathan, Pineda, Juan A., Fortea, Juan, Popp, Julius, Deckert, Jürgen, Buerger, Katharina, Morgan, Kevin, Fließbach, Klaus, Sleegers, Kristel, Molina-Porcel, Laura, Kilander, Lena, Weinhold, Leonie, Farrer, Lindsay A., Wang, Li-San, Kleineidam, Luca, Farotti, Lucia, Parnetti, Lucilla, Tremolizzo, Lucio, Hausner, Lucrezia, Benussi, Luisa, Froelich, Lutz, Ikram, M. Arfan, Deniz-Naranjo, M. Candida, Tsolaki, Magda, Rosende-Roca, Maitée, Löwenmark, Malin, Hulsman, Marc, Spallazzi, Marco, Pericak-Vance, Margaret A., Esiri, Margaret, Bernal Sánchez-Arjona, María, Dalmasso, Maria Carolina, Martínez-Larrad, María Teresa, Arcaro, Marina, Nöthen, Markus M., Fernández-Fuertes, Marta, Dichgans, Martin, Ingelsson, Martin, Herrmann, Martin J., Scherer, Martin, Vyhnalek, Martin, Kosmidis, Mary H., Yannakoulia, Mary, Schmid, Matthias, Ewers, Michael, Heneka, Michael T., Wagner, Michael, Scamosci, Michela, Kivipelto, Miia, Hiltunen, Mikko, Zulaica, Miren, Alegret, Montserrat, Fornage, Myriam, Roberto, Natalia, van Schoor, Natasja M., Seidu, Nazib M., Banaj, Nerisa, Armstrong, Nicola J., Scarmeas, Nikolaos, Scherbaum, Norbert, Goldhardt, Oliver, Hanon, Oliver, Peters, Oliver, Skrobot, Olivia Anna, Quenez, Olivier, Lerch, Ondrej, Bossù, Paola, Caffarra, Paolo, Dionigi Rossi, Paolo, Sakka, Paraskevi, Mecocci, Patrizia, Hoffmann, Per, Holmans, Peter A., Fischer, Peter, Riederer, Peter, Yang, Qiong, Marshall, Rachel, Kalaria, Rajesh N., Mayeux, Richard, Vandenberghe, Rik, Cecchetti, Roberta, Ghidoni, Roberta, Frikke-Schmidt, Ruth, Sorbi, Sandro, Hägg, Sara, Engelborghs, Sebastiaan, Helisalmi, Seppo, Botne Sando, Sigrid, Kern, Silke, Archetti, Silvana, Boschi, Silvia, Fostinelli, Silvia, Gil, Silvia, Mendoza, Silvia, Mead, Simon, Ciccone, Simona, Djurovic, Srdjan, Heilmann-Heimbach, Stefanie, Riedel-Heller, Steffi, Kuulasmaa, Teemu, del Ser, Teodoro, Lebouvier, Thibaud, Polak, Thomas, Ngandu, Tiia, Grimmer, Timo, Bessi, Valentina, Escott-Price, Valentina, Giedraitis, Vilmantas, Deramecourt, Vincent, Maier, Wolfgang, Jian, Xueqiu, Pijnenburg, Yolande A. L., Smith, A. David, Saenz, Aldo, Bizzarro, Alessandra, Lauria, Alessandra, Vacca, Alessandro, Solomon, Alina, Anastasiou, Anna, Richardson, Anna, Boland, Anne, Koivisto, Anne, Daniele, Antonio, Greco, Antonio, Marianthi, Arnaoutoglou, McGuinness, Bernadette, Fin, Bertrand, Ferrari, Camilla, Custodero, Carlo, Ferrarese, Carlo, Ingino, Carlos, Mangone, Carlos, Reyes Toso, Carlos, Martínez, Carmen, Cuesta, Carolina, Muchnik, Carolina, Joachim, Catharine, Ortiz, Cecilia, Besse, Céline, Johansson, Charlotte, Zoia, Chiara Paola, Laske, Christoph, Anastasiou, Costas, Palacio, Dana Lis, Politis, Daniel G., Janowitz, Daniel, Craig, David, Mann, David M., Neary, David, Jürgen, Deckert, Daian, Delphine, Belezhanska, Diyana, Kohler, Eduardo, Castaño, Eduardo M., Koutsouraki, Effrosyni, Chipi, Elena, De Roeck, Ellen, Costantini, Emanuele, Vardy, Emma R. L. C., Piras, Fabrizio, Roveta, Fausto, Piras, Federica, Prestia, Federico Ariel, Assogna, Francesca, Salani, Francesca, Sala, Gessica, Lacidogna, Giordano, Novack, Gisela, Wilcock, Gordon, Thonberg, Håkan, Kölsch, Heike, Weber, Heike, Boecker, Henning, Etchepareborda, Ignacio, Piaceri, Irene, Tuomilehto, Jaakko, Lindström, Jaana, Laczo, Jan, Johnston, Janet, Deleuze, Jean-François, Harris, Jenny, Schott, Jonathan M., Priller, Josef, Bacha, Juan Ignacio, Snowden, Julie, Lisso, Julieta, Mihova, Kalina Yonkova, Traykov, Latchezar, Morelli, Laura, Brusco, Luis Ignacio, Rainer, Malik, Takalo, Mari, Bjerke, Maria, Del Zompo, Maria, Serpente, Maria, Sanchez Abalos, Mariana, Rios, Mario, Peltonen, Markku, Herrman, Martin J., Kohler, Matias, Rojo, Matias, Jones, Matthew, Orsini, Michela, Medel, Nancy, Olivar, Natividad, Fox, Nick C., Salvadori, Nicola, Hooper, Nigel M., Galeano, Pablo, Solis, Patricia, Bastiani, Patrizia, Passmore, Peter, Heun, Reinhard, Antikainen, Riitta, Olaso, Robert, Perneczky, Robert, Germani, Sandra, López-García, Sara, Love, Seth, Mehrabian, Shima, Bagnoli, Silvia, Kochen, Silvia, Andreoni, Simona, Teipel, Stefan, Todd, Stephen, Pickering-Brown, Stuart, Natunen, Teemu, Tegos, Thomas, Laatikainen, Tiina, Strandberg, Timo, Polvikoski, Tuomo M., Matoska, Vaclav, Ciullo, Valentina, Cores, Valeria, Solfrizzi, Vincenzo, Lisetti, Viviana, Sevillano, Zulma, Abdelnour, C., Aguilera, N., Alarcon, E., Alegret, M., Benaque, A., Boada, M., Buendia, M., Cañabate, P., Carracedo, A., de Rojas, I., Diego, S., Espinosa, A., Gailhajenet, A., García-González, P., Gil, S., Guitart, M., González-Pérez, A., Hernández, I., Ibarria, M., Lafuente, A., Macias, J., Maroñas, O., Martín, E., Martínez, M.T., Marquié, M., Mauleón, A., Montrreal, L., Moreno-Grau, S., Moreno, M., Orellana, A., Ortega, G., Pancho, A., Pelejá, E., Pérez-Cordon, A., Pineda, J.A., Preckler, S., Quintela, I., Real, L.M., Rosende-Roca, M., Ruiz, A., Sáez, M.E., Sanabria, A., Serrano-Rios, M., Sotolongo-Grau, O., Tárraga, L., Valero, S., Vargas, L., Adarmes-Gómez, A.D., Alarcón-Martín, E., Alonso, M.D., Álvarez, I., Álvarez, V., Amer-Ferrer, G., Antequera, M., Antúnez, C., Baquero, M., Bernal, M., Blesa, R., Bullido, M.J., Burguera, J.A., Calero, M., Carrillo, F., Carrión-Claro, M., Casajeros, M.J., Clarimón, J., Cruz-Gamero, J.M., de Pancorbo, M.M., del Ser, T., Diez-Fairen, M., Escuela, R., Garrote-Espina, L., Fortea, J., Franco-Macías, E., Frank-García, A., Garcia Madrona, S., Gómez-Garre, P., Hevilla, S., Jesús, S., Labrador Espinosa, M.A., Lage, C., Legaz, A., Lleó, A., Lopez de Munain, A., López-García, S., Macias-García, D., Manzanares, S., Marín, M., Marín-Muñoz, J., Marín, T., Martín Montes, A., Martínez, B., Martínez, C., Martínez, V., Martínez-Lage Álvarez, P., Medina, M., Mendioroz Iriarte, M., Menéndez-González, M., Mir, P., Molinuevo, J.L., Pastor, P., Pérez Tur, J., Periñán-Tocino, T., Pineda-Sanchez, R., Piñol-Ripoll, G., Rábano, A., Real de Asúa, D., Rodrigo, S., Rodríguez-Rodríguez, E., Royo, J.L., Sanchez del Valle Díaz, R., Sánchez-Juan, P., Sastre, I., Vicente, M.P., Vigo-Ortega, R., Vivancos, L., Macleod, C., McCracken, C., Brayne, Carol, Bresner, Catherine, Grozeva, Detelina, Bellou, Eftychia, Sommerville, Ewen W., Matthews, F., Leonenko, Ganna, Menzies, Georgina, Windle, Gill, Harwood, Janet, Phillips, Judith, Bennett, K., Luckuck, Lauren, Clare, Linda, Woods, Robert, Saad, Salha, Burholt, Vanessa, Kehoe, Patrick Gavin, Garcia-Ribas, Guillermo, Sánchez-Juan, Pascual, Pastor, Pau, Pérez-Tur, Jordi, Piñol-Ripoll, Gerard, Lopez de Munain, Adolfo, García-Alberca, Jose María, Bullido, María J., Álvarez, Victoria, Lleó, Alberto, Real, Luis M., Mir, Pablo, Medina, Miguel, Scheltens, Philip, Holstege, Henne, Marquié, Marta, Sáez, María Eugenia, Carracedo, Ángel, Amouyel, Philippe, Schellenberg, Gerard D., Williams, Julie, Seshadri, Sudha, van Duijn, Cornelia M., Mather, Karen A., Sánchez-Valle, Raquel, Serrano-Ríos, Manuel, Orellana, Adelina, Tárraga, Lluís, Blennow, Kaj, Huisman, Martijn, Andreassen, Ole A., Posthuma, Danielle, Clarimón, Jordi, Boada, Mercè, van der Flier, Wiesje M., Ramirez, Alfredo, Lambert, Jean-Charles, van der Lee, Sven J., Ruiz, Agustín, Smith, A David, Saenz, Aldo, Bizzarro, Alessandra, Lauria, Alessandra, Vacca, Alessandro, Solomon, Alina, Anastasiou, Anna, Richardson, Anna, Boland, Anne, Koivisto, Anne, Daniele, Antonio, Greco, Antonio, Marianthi, Arnaoutoglou, McGuinness, Bernadette, Fin, Bertrand, Ferrari, Camilla, Custodero, Carlo, Ferrarese, Carlo, Ingino, Carlos, Mangone, Carlos, Reyes Toso, Carlos, Martínez, Carmen, Cuesta, Carolina, Muchnik, Carolina, Joachim, Catharine, Ortiz, Cecilia, Besse, Céline, Johansson, Charlotte, Zoia, Chiara Paola, Laske, Christoph, Anastasiou, Costas, Palacio, Dana Lis, Politis, Daniel G, Janowitz, Daniel, Craig, David, Mann, David M, Neary, David, Jürgen, Deckert, Daian, Delphine, Belezhanska, Diyana, Kohler, Eduardo, Castaño, Eduardo M, Koutsouraki, Effrosyni, Chipi, Elena, De Roeck, Ellen, Costantini, Emanuele, Vardy, Emma R L C, Piras, Fabrizio, Roveta, Fausto, Piras, Federica, Prestia, Federico Ariel, Assogna, Francesca, Salani, Francesca, Sala, Gessica, Lacidogna, Giordano, Novack, Gisela, Wilcock, Gordon, Thonberg, Håkan, Kölsch, Heike, Weber, Heike, Boecker, Henning, Etchepareborda, Ignacio, Piaceri, Irene, Tuomilehto, Jaakko, Lindström, Jaana, Laczo, Jan, Johnston, Janet, Deleuze, Jean-François, Harris, Jenny, Schott, Jonathan M, Priller, Josef, Bacha, Juan Ignacio, Snowden, Julie, Lisso, Julieta, Mihova, Kalina Yonkova, Traykov, Latchezar, Morelli, Laura, Brusco, Luis Ignacio, Rainer, Malik, Takalo, Mari, Bjerke, Maria, Del Zompo, Maria, Serpente, Maria, Sanchez Abalos, Mariana, Rios, Mario, Peltonen, Markku, Herrman, Martin J, Kohler, Matias, Rojo, Matias, Jones, Matthew, Orsini, Michela, Medel, Nancy, Olivar, Natividad, Fox, Nick C, Salvadori, Nicola, Hooper, Nigel M, Galeano, Pablo, Solis, Patricia, Bastiani, Patrizia, Passmore, Peter, Heun, Reinhard, Antikainen, Riitta, Olaso, Robert, Perneczky, Robert, Germani, Sandra, López-García, Sara, Love, Seth, Mehrabian, Shima, Bagnoli, Silvia, Kochen, Silvia, Andreoni, Simona, Teipel, Stefan, Todd, Stephen, Pickering-Brown, Stuart, Natunen, Teemu, Tegos, Thomas, Laatikainen, Tiina, Strandberg, Timo, Polvikoski, Tuomo M, Matoska, Vaclav, Ciullo, Valentina, Cores, Valeria, Solfrizzi, Vincenzo, Lisetti, Viviana, Sevillano, Zulma, Abdelnour, C., Aguilera, N., Alarcon, E., Alegret, M., Benaque, A., Boada, M., Buendia, M., Cañabate, P., Carracedo, A., Corbatón-Anchuelo, A., de Rojas, I., Diego, S., Espinosa, A., Gailhajenet, A., García-González, P., Gil, S., Guitart, M., González-Pérez, A., Hernández, I., Ibarria, M., Lafuente, A., Macias, J., Maroñas, O., Martín, E., Martínez, M. T., Marquié, M., Mauleón, A., Montrreal, L., Moreno-Grau, S., Moreno, M., Orellana, A., Ortega, G., Pancho, A., Pelejá, E., Pérez-Cordon, A., Pineda, J. A., Preckler, S., Quintela, I., Real, L. M., Rosende-Roca, M., Ruiz, A., Sáez, M. E., Sanabria, A., Serrano-Rios, M., Sotolongo-Grau, O., Tárraga, L., Valero, S., Vargas, L., Adarmes-Gómez, A. D., Alarcón-Martín, E., Alonso, M. D., Álvarez, I., Álvarez, V., Amer-Ferrer, G., Antequera, M., Antúnez, C., Baquero, M., Bernal, M., Blesa, R., Buiza-Rueda, D., Bullido, M. J., Burguera, J. A., Calero, M., Carrillo, F., Carrión-Claro, M., Casajeros, M. J., Clarimón, J., Cruz-Gamero, J. M., de Pancorbo, M. M., Del Ser, T., Diez-Fairen, M., Escuela, R., Garrote-Espina, L., Fortea, J., Franco-Macías, E., Frank-García, A., García-Alberca, J. M., Garcia Madrona, S., Garcia-Ribas, G., Gómez-Garre, P., Hevilla, S., Jesús, S., Labrador Espinosa, M. A., Lage, C., Legaz, A., Lleó, A., Lopez de Munain, A., López-García, S., Macias-García, D., Manzanares, S., Marín, M., Marín-Muñoz, J., Marín, T., Martín Montes, A., Martínez, B., Martínez, C., Martínez, V., Martínez-Lage Álvarez, P., Medina, M., Mendioroz Iriarte, M., Menéndez-González, M., Mir, P., Molinuevo, J. L., Pastor, P., Pérez Tur, J., Periñán-Tocino, T., Pineda-Sanchez, R., Piñol-Ripoll, G., Rábano, A., Real de Asúa, D., Rodrigo, S., Rodríguez-Rodríguez, E., Royo, J. L., Sanchez Del Valle Díaz, R., Sánchez-Juan, P., Sastre, I., Vicente, M. P., Vigo-Ortega, R., Vivancos, L., Macleod, C., McCracken, C., Brayne, Carol, Bresner, Catherine, Grozeva, Detelina, Bellou, Eftychia, Sommerville, Ewen W, Matthews, F., Leonenko, Ganna, Menzies, Georgina, Windle, Gill, Harwood, Janet, Phillips, Judith, Bennett, K., Luckuck, Lauren, Clare, Linda, Woods, Robert, Saad, Salha, Burholt, Vanessa, Rongve, Arvid, Brussels Heritage Lab, Clinical sciences, Neuroprotection & Neuromodulation, and Neurology

Subjects
polygenic risk scores, Multidisciplinary, Common variants, Neuroscience(all), neurology, Medizin, General Physics and Astronomy, ddc:500, General Chemistry, Alzheimer's disease, General Biochemistry, Genetics and Molecular Biology, RISK STRATIFICATION
Abstract

The original version of this Article omitted from the author list the 212th author Patrizia Mecocci, who is from the Institute of Gerontology and Geriatrics, Department of Medicine, University of Perugia, Perugia, Italy. Consequently, the “Sample Contribution” section of Author Contributions was updated to add “P.M” between “P.D.” and “R.C.”. Additionally, the original version of this Article contained the incorrect affiliation for author Patrick Gavin Kehoe, which incorrectly read “German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany”. The correct version replaces this affiliation with “Bristol Medical School (THS), University of Bristol, Southmead Hospital, Bristol, UK”. This has been corrected in both the PDF and HTML versions of the Article. CA extern

Published
2023

6. sj-pdf-1-jcb-10.1177_0271678X231159958 - Supplemental material for Harmonization of sensorimotor deficit assessment in a registered multicentre pre-clinical randomized controlled trial using two models of ischemic stroke

Author

Valente, Alessia, Mariani, Jacopo, Seminara, Serena, Tettamanti, Mauro, Pignataro, Giuseppe, Perego, Carlo, Sironi, Luigi, Pedata, Felicita, Amantea, Diana, Bacigaluppi, Marco, Vinciguerra, Antonio, Diamanti, Susanna, Viganò, Martina, Santangelo, Francesco, Zoia, Chiara Paola, Rodriguez-Menendez, Virginia, Castiglioni, Laura, Rzemieniec, Joanna, Dettori, Ilaria, Bulli, Irene, Coppi, Elisabetta, Di Santo, Chiara, Cuomo, Ornella, Gullotta, Giorgia Serena, Butti, Erica, Bagetta, Giacinto, Martino, Gianvito, De Simoni, Maria-Grazia, Ferrarese, Carlo, Fumagalli, Stefano, and Beretta, Simone

Subjects
110320 Radiology and Organ Imaging, FOS: Clinical medicine, FOS: Biological sciences, Medicine, Cell Biology, 110305 Emergency Medicine, 110306 Endocrinology, Biochemistry, 69999 Biological Sciences not elsewhere classified, 110904 Neurology and Neuromuscular Diseases, Neuroscience
Abstract

Supplemental material, sj-pdf-1-jcb-10.1177_0271678X231159958 for Harmonization of sensorimotor deficit assessment in a registered multicentre pre-clinical randomized controlled trial using two models of ischemic stroke by Alessia Valente, Jacopo Mariani, Serena Seminara, Mauro Tettamanti, Giuseppe Pignataro, Carlo Perego, Luigi Sironi, Felicita Pedata, Diana Amantea, Marco Bacigaluppi, Antonio Vinciguerra, Susanna Diamanti, Martina Viganò, Francesco Santangelo, Chiara Paola Zoia, Virginia Rodriguez-Menendez, Laura Castiglioni, Joanna Rzemieniec, Ilaria Dettori, Irene Bulli, Elisabetta Coppi, Chiara Di Santo, Ornella Cuomo, Giorgia Serena Gullotta, Erica Butti, Giacinto Bagetta, Gianvito Martino, Maria-Grazia De Simoni, Carlo Ferrarese, Stefano Fumagalli, Simone Beretta and for the TRICS study group in Journal of Cerebral Blood Flow & Metabolism

Published
2023
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7. Alzheimer's Disease Prevention through Natural Compounds: Cell- Free, In Vitro, and In Vivo Dissection of Hop (Humulus lupulus L.) Multitarget Activity

Author

Palmioli, Alessandro, Mazzoni, Valeria, De Luigi, Ada, Bruzzone, Chiara, Sala, Gessica, Colombo, Laura, Bazzini, Chiara, Zoia, Chiara Paola, Inserra Maria Giovanna, Salmona, Mario, De Noni, Ivano, Ferrarese, Carlo, Diomede, Luisa, and Airoldi, Cristina

Subjects
hop, UPLC-HR-MS, anti-Aβ compounds, Alzheimer's disease, Caenorhabditis elegans, NMR
Abstract

The relevant social and economic costs associated with aging and neurodegenerative diseases, particularly Alzheimer’s disease (AD), entail considerable efforts to develop effective preventive and therapeutic strategies. The search for natural compounds, whose intake through diet can help prevent the main biochemical mechanisms responsible for AD onset, led us to screen hops, one of the main ingredients of beer. To explore the chemical variability of hops, we characterized four hop varieties, i.e., Cascade, Saaz, Tettnang, and Summit. We investigated the potential multitarget hop activity, in particular its ability to hinder Aβ1− 42 peptide aggregation and cytotoxicity, its antioxidant properties, and its ability to enhance autophagy, promoting the clearance of misfolded and aggregated proteins in a human neuroblastoma SH-SY5Y cell line. Moreover, we provided evidence of in vivo hop efficacy using the transgenic CL2006Caenorhabditis elegans strain expressing the Aβ3−42 peptide. By combining cell-free and in vitro assays with nuclear magnetic resonance (NMR) and MS-based metabolomics, NMR molecular recognition studies, and atomic force microscopy, we identified feruloyl and p-coumaroylquinic acids flavan-3-ol glycosides and procyanidins as the main anti-Aβ components of hop.

Published
2022
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8. Alzheimer’s Disease Prevention through Natural Compounds: Cell-Free, In Vitro, and In Vivo Dissection of Hop (Humulus lupulus L.) Multitarget Activity

Author

Palmioli, Alessandro, primary, Mazzoni, Valeria, additional, De Luigi, Ada, additional, Bruzzone, Chiara, additional, Sala, Gessica, additional, Colombo, Laura, additional, Bazzini, Chiara, additional, Zoia, Chiara Paola, additional, Inserra, Mariagiovanna, additional, Salmona, Mario, additional, De Noni, Ivano, additional, Ferrarese, Carlo, additional, Diomede, Luisa, additional, and Airoldi, Cristina, additional

Published
2022
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9. Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer’s Disease Patients

Author

La Rosa, Francesca, primary, Zoia, Chiara Paola, additional, Bazzini, Chiara, additional, Bolognini, Alessandra, additional, Saresella, Marina, additional, Conti, Elisa, additional, Ferrarese, Carlo, additional, Piancone, Federica, additional, Marventano, Ivana, additional, Galimberti, Daniela, additional, Fenoglio, Chiara, additional, Scarpini, Elio, additional, and Clerici, Mario, additional

Published
2022
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11. Common variants in Alzheimer's disease and risk stratification by polygenic risk scores

Author

de Rojas, I, Moreno-Grau, S, Tesi, N, Grenier-Boley, B, Andrade, V, Jansen, I, Pedersen, N, Stringa, N, Zettergren, A, Hernández, I, Montrreal, L, Antúnez, C, Antonell, A, Tankard, R, Bis, J, Sims, R, Bellenguez, C, Quintela, I, González-Perez, A, Calero, M, Franco-Macías, E, Macías, J, Blesa, R, Cervera-Carles, L, Menéndez-González, M, Frank-García, A, Royo, J, Moreno, F, Huerto Vilas, R, Baquero, M, Diez-Fairen, M, Lage, C, García-Madrona, S, García-González, P, Alarcón-Martín, E, Valero, S, Sotolongo-Grau, O, Ullgren, A, Naj, A, Lemstra, A, Benaque, A, Pérez-Cordón, A, Benussi, A, Rábano, A, Padovani, A, Squassina, A, de Mendonça, A, Arias Pastor, A, Kok, A, Meggy, A, Pastor, A, Espinosa, A, Corma-Gómez, A, Martín Montes, A, Sanabria, Á, Destefano, A, Schneider, A, Haapasalo, A, Kinhult Ståhlbom, A, Tybjærg-Hansen, A, Hartmann, A, Spottke, A, Corbatón-Anchuelo, A, Rongve, A, Borroni, B, Arosio, B, Nacmias, B, Nordestgaard, B, Kunkle, B, Charbonnier, C, Abdelnour, C, Masullo, C, Martínez Rodríguez, C, Muñoz-Fernandez, C, Dufouil, C, Graff, C, Ferreira, C, Chillotti, C, Reynolds, C, Fenoglio, C, Van Broeckhoven, C, Clark, C, Pisanu, C, Satizabal, C, Holmes, C, Buiza-Rueda, D, Aarsland, D, Rujescu, D, Alcolea, D, Galimberti, D, Wallon, D, Seripa, D, Grünblatt, E, Dardiotis, E, Düzel, E, Scarpini, E, Conti, E, Rubino, E, Gelpi, E, Rodriguez-Rodriguez, E, Duron, E, Boerwinkle, E, Ferri, E, Tagliavini, F, Küçükali, F, Pasquier, F, Sanchez-Garcia, F, Mangialasche, F, Jessen, F, Nicolas, G, Selbæk, G, Ortega, G, Chêne, G, Hadjigeorgiou, G, Rossi, G, Spalletta, G, Giaccone, G, Grande, G, Binetti, G, Papenberg, G, Hampel, H, Bailly, H, Zetterberg, H, Soininen, H, Karlsson, I, Alvarez, I, Appollonio, I, Giegling, I, Skoog, I, Saltvedt, I, Rainero, I, Rosas Allende, I, Hort, J, Diehl-Schmid, J, Van Dongen, J, Vidal, J, Lehtisalo, J, Wiltfang, J, Thomassen, J, Kornhuber, J, Haines, J, Vogelgsang, J, Pineda, J, Fortea, J, Popp, J, Deckert, J, Buerger, K, Morgan, K, Fließbach, K, Sleegers, K, Molina-Porcel, L, Kilander, L, Weinhold, L, Farrer, L, Wang, L, Kleineidam, L, Farotti, L, Parnetti, L, Tremolizzo, L, Hausner, L, Benussi, L, Froelich, L, Ikram, M, Deniz-Naranjo, M, Tsolaki, M, Rosende-Roca, M, Löwenmark, M, Hulsman, M, Spallazzi, M, Pericak-Vance, M, Esiri, M, Bernal Sánchez-Arjona, M, Dalmasso, M, Martínez-Larrad, M, Arcaro, M, Nöthen, M, Fernández-Fuertes, M, Dichgans, M, Ingelsson, M, Herrmann, M, Scherer, M, Vyhnalek, M, Kosmidis, M, Yannakoulia, M, Schmid, M, Ewers, M, Heneka, M, Wagner, M, Scamosci, M, Kivipelto, M, Hiltunen, M, Zulaica, M, Alegret, M, Fornage, M, Roberto, N, van Schoor, N, Seidu, N, Banaj, N, Armstrong, N, Scarmeas, N, Scherbaum, N, Goldhardt, O, Hanon, O, Peters, O, Skrobot, O, Quenez, O, Lerch, O, Bossù, P, Caffarra, P, Dionigi Rossi, P, Sakka, P, Hoffmann, P, Holmans, P, Fischer, P, Riederer, P, Yang, Q, Marshall, R, Kalaria, R, Mayeux, R, Vandenberghe, R, Cecchetti, R, Ghidoni, R, Frikke-Schmidt, R, Sorbi, S, Hägg, S, Engelborghs, S, Helisalmi, S, Botne Sando, S, Kern, S, Archetti, S, Boschi, S, Fostinelli, S, Gil, S, Mendoza, S, Mead, S, Ciccone, S, Djurovic, S, Heilmann-Heimbach, S, Riedel-Heller, S, Kuulasmaa, T, Del Ser, T, Lebouvier, T, Polak, T, Ngandu, T, Grimmer, T, Bessi, V, Escott-Price, V, Giedraitis, V, Deramecourt, V, Maier, W, Jian, X, Pijnenburg, Y, Andreoni, S, Ferrarese, C, Sala, G, Zoia, C, Kehoe, P, Garcia-Ribas, G, Sánchez-Juan, P, Pastor, P, Pérez-Tur, J, Piñol-Ripoll, G, Lopez de Munain, A, García-Alberca, J, Bullido, M, Álvarez, V, Lleó, A, Real, L, Mir, P, Medina, M, Scheltens, P, Holstege, H, Marquié, M, Sáez, M, Carracedo, Á, Amouyel, P, Schellenberg, G, Williams, J, Seshadri, S, van Duijn, C, Mather, K, Sánchez-Valle, R, Serrano-Ríos, M, Orellana, A, Tárraga, L, Blennow, K, Huisman, M, Andreassen, O, Posthuma, D, Clarimón, J, Boada, M, van der Flier, W, Ramirez, A, Lambert, J, van der Lee, S, Ruiz, A, de Rojas, Itziar, Moreno-Grau, Sonia, Tesi, Niccolo, Grenier-Boley, Benjamin, Andrade, Victor, Jansen, Iris E, Pedersen, Nancy L, Stringa, Najada, Zettergren, Anna, Hernández, Isabel, Montrreal, Laura, Antúnez, Carmen, Antonell, Anna, Tankard, Rick M, Bis, Joshua C, Sims, Rebecca, Bellenguez, Céline, Quintela, Inés, González-Perez, Antonio, Calero, Miguel, Franco-Macías, Emilio, Macías, Juan, Blesa, Rafael, Cervera-Carles, Laura, Menéndez-González, Manuel, Frank-García, Ana, Royo, Jose Luís, Moreno, Fermin, Huerto Vilas, Raquel, Baquero, Miquel, Diez-Fairen, Mónica, Lage, Carmen, García-Madrona, Sebastián, García-González, Pablo, Alarcón-Martín, Emilio, Valero, Sergi, Sotolongo-Grau, Oscar, Ullgren, Abbe, Naj, Adam C, Lemstra, Afina W, Benaque, Alba, Pérez-Cordón, Alba, Benussi, Alberto, Rábano, Alberto, Padovani, Alessandro, Squassina, Alessio, de Mendonça, Alexandre, Arias Pastor, Alfonso, Kok, Almar A L, Meggy, Alun, Pastor, Ana Belén, Espinosa, Ana, Corma-Gómez, Anas, Martín Montes, Angel, Sanabria, Ángela, DeStefano, Anita L, Schneider, Anja, Haapasalo, Annakaisa, Kinhult Ståhlbom, Anne, Tybjærg-Hansen, Anne, Hartmann, Annette M, Spottke, Annika, Corbatón-Anchuelo, Arturo, Rongve, Arvid, Borroni, Barbara, Arosio, Beatrice, Nacmias, Benedetta, Nordestgaard, Børge G, Kunkle, Brian W, Charbonnier, Camille, Abdelnour, Carla, Masullo, Carlo, Martínez Rodríguez, Carmen, Muñoz-Fernandez, Carmen, Dufouil, Carole, Graff, Caroline, Ferreira, Catarina B, Chillotti, Caterina, Reynolds, Chandra A, Fenoglio, Chiara, Van Broeckhoven, Christine, Clark, Christopher, Pisanu, Claudia, Satizabal, Claudia L, Holmes, Clive, Buiza-Rueda, Dolores, Aarsland, Dag, Rujescu, Dan, Alcolea, Daniel, Galimberti, Daniela, Wallon, David, Seripa, Davide, Grünblatt, Edna, Dardiotis, Efthimios, Düzel, Emrah, Scarpini, Elio, Conti, Elisa, Rubino, Elisa, Gelpi, Ellen, Rodriguez-Rodriguez, Eloy, Duron, Emmanuelle, Boerwinkle, Eric, Ferri, Evelyn, Tagliavini, Fabrizio, Küçükali, Fahri, Pasquier, Florence, Sanchez-Garcia, Florentino, Mangialasche, Francesca, Jessen, Frank, Nicolas, Gaël, Selbæk, Geir, Ortega, Gemma, Chêne, Geneviève, Hadjigeorgiou, Georgios, Rossi, Giacomina, Spalletta, Gianfranco, Giaccone, Giorgio, Grande, Giulia, Binetti, Giuliano, Papenberg, Goran, Hampel, Harald, Bailly, Henri, Zetterberg, Henrik, Soininen, Hilkka, Karlsson, Ida K, Alvarez, Ignacio, Appollonio, Ildebrando, Giegling, Ina, Skoog, Ingmar, Saltvedt, Ingvild, Rainero, Innocenzo, Rosas Allende, Irene, Hort, Jakub, Diehl-Schmid, Janine, Van Dongen, Jasper, Vidal, Jean-Sebastien, Lehtisalo, Jenni, Wiltfang, Jens, Thomassen, Jesper Qvist, Kornhuber, Johannes, Haines, Jonathan L, Vogelgsang, Jonathan, Pineda, Juan A, Fortea, Juan, Popp, Julius, Deckert, Jürgen, Buerger, Katharina, Morgan, Kevin, Fließbach, Klaus, Sleegers, Kristel, Molina-Porcel, Laura, Kilander, Lena, Weinhold, Leonie, Farrer, Lindsay A, Wang, Li-San, Kleineidam, Luca, Farotti, Lucia, Parnetti, Lucilla, Tremolizzo, Lucio, Hausner, Lucrezia, Benussi, Luisa, Froelich, Lutz, Ikram, M Arfan, Deniz-Naranjo, M Candida, Tsolaki, Magda, Rosende-Roca, Maitée, Löwenmark, Malin, Hulsman, Marc, Spallazzi, Marco, Pericak-Vance, Margaret A, Esiri, Margaret, Bernal Sánchez-Arjona, María, Dalmasso, Maria Carolina, Martínez-Larrad, María Teresa, Arcaro, Marina, Nöthen, Markus M, Fernández-Fuertes, Marta, Dichgans, Martin, Ingelsson, Martin, Herrmann, Martin J, Scherer, Martin, Vyhnalek, Martin, Kosmidis, Mary H, Yannakoulia, Mary, Schmid, Matthias, Ewers, Michael, Heneka, Michael T, Wagner, Michael, Scamosci, Michela, Kivipelto, Miia, Hiltunen, Mikko, Zulaica, Miren, Alegret, Montserrat, Fornage, Myriam, Roberto, Natalia, van Schoor, Natasja M, Seidu, Nazib M, Banaj, Nerisa, Armstrong, Nicola J, Scarmeas, Nikolaos, Scherbaum, Norbert, Goldhardt, Oliver, Hanon, Oliver, Peters, Oliver, Skrobot, Olivia Anna, Quenez, Olivier, Lerch, Ondrej, Bossù, Paola, Caffarra, Paolo, Dionigi Rossi, Paolo, Sakka, Paraskevi, Hoffmann, Per, Holmans, Peter A, Fischer, Peter, Riederer, Peter, Yang, Qiong, Marshall, Rachel, Kalaria, Rajesh N, Mayeux, Richard, Vandenberghe, Rik, Cecchetti, Roberta, Ghidoni, Roberta, Frikke-Schmidt, Ruth, Sorbi, Sandro, Hägg, Sara, Engelborghs, Sebastiaan, Helisalmi, Seppo, Botne Sando, Sigrid, Kern, Silke, Archetti, Silvana, Boschi, Silvia, Fostinelli, Silvia, Gil, Silvia, Mendoza, Silvia, Mead, Simon, Ciccone, Simona, Djurovic, Srdjan, Heilmann-Heimbach, Stefanie, Riedel-Heller, Steffi, Kuulasmaa, Teemu, Del Ser, Teodoro, Lebouvier, Thibaud, Polak, Thomas, Ngandu, Tiia, Grimmer, Timo, Bessi, Valentina, Escott-Price, Valentina, Giedraitis, Vilmantas, Deramecourt, Vincent, Maier, Wolfgang, Jian, Xueqiu, Pijnenburg, Yolande A L, Andreoni, Simona, Ferrarese, Carlo, Sala Gessica, Zoia, Chiara Paola, Kehoe, Patrick Gavin, Garcia-Ribas, Guillermo, Sánchez-Juan, Pascual, Pastor, Pau, Pérez-Tur, Jordi, Piñol-Ripoll, Gerard, Lopez de Munain, Adolfo, García-Alberca, Jose María, Bullido, María J, Álvarez, Victoria, Lleó, Alberto, Real, Luis M, Mir, Pablo, Medina, Miguel, Scheltens, Philip, Holstege, Henne, Marquié, Marta, Sáez, María Eugenia, Carracedo, Ángel, Amouyel, Philippe, Schellenberg, Gerard D, Williams, Julie, Seshadri, Sudha, van Duijn, Cornelia M, Mather, Karen A, Sánchez-Valle, Raquel, Serrano-Ríos, Manuel, Orellana, Adelina, Tárraga, Lluís, Blennow, Kaj, Huisman, Martijn, Andreassen, Ole A, Posthuma, Danielle, Clarimón, Jordi, Boada, Mercè, van der Flier, Wiesje M, Ramirez, Alfredo, Lambert, Jean-Charles, van der Lee, Sven J, Ruiz, Agustín, de Rojas, I, Moreno-Grau, S, Tesi, N, Grenier-Boley, B, Andrade, V, Jansen, I, Pedersen, N, Stringa, N, Zettergren, A, Hernández, I, Montrreal, L, Antúnez, C, Antonell, A, Tankard, R, Bis, J, Sims, R, Bellenguez, C, Quintela, I, González-Perez, A, Calero, M, Franco-Macías, E, Macías, J, Blesa, R, Cervera-Carles, L, Menéndez-González, M, Frank-García, A, Royo, J, Moreno, F, Huerto Vilas, R, Baquero, M, Diez-Fairen, M, Lage, C, García-Madrona, S, García-González, P, Alarcón-Martín, E, Valero, S, Sotolongo-Grau, O, Ullgren, A, Naj, A, Lemstra, A, Benaque, A, Pérez-Cordón, A, Benussi, A, Rábano, A, Padovani, A, Squassina, A, de Mendonça, A, Arias Pastor, A, Kok, A, Meggy, A, Pastor, A, Espinosa, A, Corma-Gómez, A, Martín Montes, A, Sanabria, Á, Destefano, A, Schneider, A, Haapasalo, A, Kinhult Ståhlbom, A, Tybjærg-Hansen, A, Hartmann, A, Spottke, A, Corbatón-Anchuelo, A, Rongve, A, Borroni, B, Arosio, B, Nacmias, B, Nordestgaard, B, Kunkle, B, Charbonnier, C, Abdelnour, C, Masullo, C, Martínez Rodríguez, C, Muñoz-Fernandez, C, Dufouil, C, Graff, C, Ferreira, C, Chillotti, C, Reynolds, C, Fenoglio, C, Van Broeckhoven, C, Clark, C, Pisanu, C, Satizabal, C, Holmes, C, Buiza-Rueda, D, Aarsland, D, Rujescu, D, Alcolea, D, Galimberti, D, Wallon, D, Seripa, D, Grünblatt, E, Dardiotis, E, Düzel, E, Scarpini, E, Conti, E, Rubino, E, Gelpi, E, Rodriguez-Rodriguez, E, Duron, E, Boerwinkle, E, Ferri, E, Tagliavini, F, Küçükali, F, Pasquier, F, Sanchez-Garcia, F, Mangialasche, F, Jessen, F, Nicolas, G, Selbæk, G, Ortega, G, Chêne, G, Hadjigeorgiou, G, Rossi, G, Spalletta, G, Giaccone, G, Grande, G, Binetti, G, Papenberg, G, Hampel, H, Bailly, H, Zetterberg, H, Soininen, H, Karlsson, I, Alvarez, I, Appollonio, I, Giegling, I, Skoog, I, Saltvedt, I, Rainero, I, Rosas Allende, I, Hort, J, Diehl-Schmid, J, Van Dongen, J, Vidal, J, Lehtisalo, J, Wiltfang, J, Thomassen, J, Kornhuber, J, Haines, J, Vogelgsang, J, Pineda, J, Fortea, J, Popp, J, Deckert, J, Buerger, K, Morgan, K, Fließbach, K, Sleegers, K, Molina-Porcel, L, Kilander, L, Weinhold, L, Farrer, L, Wang, L, Kleineidam, L, Farotti, L, Parnetti, L, Tremolizzo, L, Hausner, L, Benussi, L, Froelich, L, Ikram, M, Deniz-Naranjo, M, Tsolaki, M, Rosende-Roca, M, Löwenmark, M, Hulsman, M, Spallazzi, M, Pericak-Vance, M, Esiri, M, Bernal Sánchez-Arjona, M, Dalmasso, M, Martínez-Larrad, M, Arcaro, M, Nöthen, M, Fernández-Fuertes, M, Dichgans, M, Ingelsson, M, Herrmann, M, Scherer, M, Vyhnalek, M, Kosmidis, M, Yannakoulia, M, Schmid, M, Ewers, M, Heneka, M, Wagner, M, Scamosci, M, Kivipelto, M, Hiltunen, M, Zulaica, M, Alegret, M, Fornage, M, Roberto, N, van Schoor, N, Seidu, N, Banaj, N, Armstrong, N, Scarmeas, N, Scherbaum, N, Goldhardt, O, Hanon, O, Peters, O, Skrobot, O, Quenez, O, Lerch, O, Bossù, P, Caffarra, P, Dionigi Rossi, P, Sakka, P, Hoffmann, P, Holmans, P, Fischer, P, Riederer, P, Yang, Q, Marshall, R, Kalaria, R, Mayeux, R, Vandenberghe, R, Cecchetti, R, Ghidoni, R, Frikke-Schmidt, R, Sorbi, S, Hägg, S, Engelborghs, S, Helisalmi, S, Botne Sando, S, Kern, S, Archetti, S, Boschi, S, Fostinelli, S, Gil, S, Mendoza, S, Mead, S, Ciccone, S, Djurovic, S, Heilmann-Heimbach, S, Riedel-Heller, S, Kuulasmaa, T, Del Ser, T, Lebouvier, T, Polak, T, Ngandu, T, Grimmer, T, Bessi, V, Escott-Price, V, Giedraitis, V, Deramecourt, V, Maier, W, Jian, X, Pijnenburg, Y, Andreoni, S, Ferrarese, C, Sala, G, Zoia, C, Kehoe, P, Garcia-Ribas, G, Sánchez-Juan, P, Pastor, P, Pérez-Tur, J, Piñol-Ripoll, G, Lopez de Munain, A, García-Alberca, J, Bullido, M, Álvarez, V, Lleó, A, Real, L, Mir, P, Medina, M, Scheltens, P, Holstege, H, Marquié, M, Sáez, M, Carracedo, Á, Amouyel, P, Schellenberg, G, Williams, J, Seshadri, S, van Duijn, C, Mather, K, Sánchez-Valle, R, Serrano-Ríos, M, Orellana, A, Tárraga, L, Blennow, K, Huisman, M, Andreassen, O, Posthuma, D, Clarimón, J, Boada, M, van der Flier, W, Ramirez, A, Lambert, J, van der Lee, S, Ruiz, A, de Rojas, Itziar, Moreno-Grau, Sonia, Tesi, Niccolo, Grenier-Boley, Benjamin, Andrade, Victor, Jansen, Iris E, Pedersen, Nancy L, Stringa, Najada, Zettergren, Anna, Hernández, Isabel, Montrreal, Laura, Antúnez, Carmen, Antonell, Anna, Tankard, Rick M, Bis, Joshua C, Sims, Rebecca, Bellenguez, Céline, Quintela, Inés, González-Perez, Antonio, Calero, Miguel, Franco-Macías, Emilio, Macías, Juan, Blesa, Rafael, Cervera-Carles, Laura, Menéndez-González, Manuel, Frank-García, Ana, Royo, Jose Luís, Moreno, Fermin, Huerto Vilas, Raquel, Baquero, Miquel, Diez-Fairen, Mónica, Lage, Carmen, García-Madrona, Sebastián, García-González, Pablo, Alarcón-Martín, Emilio, Valero, Sergi, Sotolongo-Grau, Oscar, Ullgren, Abbe, Naj, Adam C, Lemstra, Afina W, Benaque, Alba, Pérez-Cordón, Alba, Benussi, Alberto, Rábano, Alberto, Padovani, Alessandro, Squassina, Alessio, de Mendonça, Alexandre, Arias Pastor, Alfonso, Kok, Almar A L, Meggy, Alun, Pastor, Ana Belén, Espinosa, Ana, Corma-Gómez, Anas, Martín Montes, Angel, Sanabria, Ángela, DeStefano, Anita L, Schneider, Anja, Haapasalo, Annakaisa, Kinhult Ståhlbom, Anne, Tybjærg-Hansen, Anne, Hartmann, Annette M, Spottke, Annika, Corbatón-Anchuelo, Arturo, Rongve, Arvid, Borroni, Barbara, Arosio, Beatrice, Nacmias, Benedetta, Nordestgaard, Børge G, Kunkle, Brian W, Charbonnier, Camille, Abdelnour, Carla, Masullo, Carlo, Martínez Rodríguez, Carmen, Muñoz-Fernandez, Carmen, Dufouil, Carole, Graff, Caroline, Ferreira, Catarina B, Chillotti, Caterina, Reynolds, Chandra A, Fenoglio, Chiara, Van Broeckhoven, Christine, Clark, Christopher, Pisanu, Claudia, Satizabal, Claudia L, Holmes, Clive, Buiza-Rueda, Dolores, Aarsland, Dag, Rujescu, Dan, Alcolea, Daniel, Galimberti, Daniela, Wallon, David, Seripa, Davide, Grünblatt, Edna, Dardiotis, Efthimios, Düzel, Emrah, Scarpini, Elio, Conti, Elisa, Rubino, Elisa, Gelpi, Ellen, Rodriguez-Rodriguez, Eloy, Duron, Emmanuelle, Boerwinkle, Eric, Ferri, Evelyn, Tagliavini, Fabrizio, Küçükali, Fahri, Pasquier, Florence, Sanchez-Garcia, Florentino, Mangialasche, Francesca, Jessen, Frank, Nicolas, Gaël, Selbæk, Geir, Ortega, Gemma, Chêne, Geneviève, Hadjigeorgiou, Georgios, Rossi, Giacomina, Spalletta, Gianfranco, Giaccone, Giorgio, Grande, Giulia, Binetti, Giuliano, Papenberg, Goran, Hampel, Harald, Bailly, Henri, Zetterberg, Henrik, Soininen, Hilkka, Karlsson, Ida K, Alvarez, Ignacio, Appollonio, Ildebrando, Giegling, Ina, Skoog, Ingmar, Saltvedt, Ingvild, Rainero, Innocenzo, Rosas Allende, Irene, Hort, Jakub, Diehl-Schmid, Janine, Van Dongen, Jasper, Vidal, Jean-Sebastien, Lehtisalo, Jenni, Wiltfang, Jens, Thomassen, Jesper Qvist, Kornhuber, Johannes, Haines, Jonathan L, Vogelgsang, Jonathan, Pineda, Juan A, Fortea, Juan, Popp, Julius, Deckert, Jürgen, Buerger, Katharina, Morgan, Kevin, Fließbach, Klaus, Sleegers, Kristel, Molina-Porcel, Laura, Kilander, Lena, Weinhold, Leonie, Farrer, Lindsay A, Wang, Li-San, Kleineidam, Luca, Farotti, Lucia, Parnetti, Lucilla, Tremolizzo, Lucio, Hausner, Lucrezia, Benussi, Luisa, Froelich, Lutz, Ikram, M Arfan, Deniz-Naranjo, M Candida, Tsolaki, Magda, Rosende-Roca, Maitée, Löwenmark, Malin, Hulsman, Marc, Spallazzi, Marco, Pericak-Vance, Margaret A, Esiri, Margaret, Bernal Sánchez-Arjona, María, Dalmasso, Maria Carolina, Martínez-Larrad, María Teresa, Arcaro, Marina, Nöthen, Markus M, Fernández-Fuertes, Marta, Dichgans, Martin, Ingelsson, Martin, Herrmann, Martin J, Scherer, Martin, Vyhnalek, Martin, Kosmidis, Mary H, Yannakoulia, Mary, Schmid, Matthias, Ewers, Michael, Heneka, Michael T, Wagner, Michael, Scamosci, Michela, Kivipelto, Miia, Hiltunen, Mikko, Zulaica, Miren, Alegret, Montserrat, Fornage, Myriam, Roberto, Natalia, van Schoor, Natasja M, Seidu, Nazib M, Banaj, Nerisa, Armstrong, Nicola J, Scarmeas, Nikolaos, Scherbaum, Norbert, Goldhardt, Oliver, Hanon, Oliver, Peters, Oliver, Skrobot, Olivia Anna, Quenez, Olivier, Lerch, Ondrej, Bossù, Paola, Caffarra, Paolo, Dionigi Rossi, Paolo, Sakka, Paraskevi, Hoffmann, Per, Holmans, Peter A, Fischer, Peter, Riederer, Peter, Yang, Qiong, Marshall, Rachel, Kalaria, Rajesh N, Mayeux, Richard, Vandenberghe, Rik, Cecchetti, Roberta, Ghidoni, Roberta, Frikke-Schmidt, Ruth, Sorbi, Sandro, Hägg, Sara, Engelborghs, Sebastiaan, Helisalmi, Seppo, Botne Sando, Sigrid, Kern, Silke, Archetti, Silvana, Boschi, Silvia, Fostinelli, Silvia, Gil, Silvia, Mendoza, Silvia, Mead, Simon, Ciccone, Simona, Djurovic, Srdjan, Heilmann-Heimbach, Stefanie, Riedel-Heller, Steffi, Kuulasmaa, Teemu, Del Ser, Teodoro, Lebouvier, Thibaud, Polak, Thomas, Ngandu, Tiia, Grimmer, Timo, Bessi, Valentina, Escott-Price, Valentina, Giedraitis, Vilmantas, Deramecourt, Vincent, Maier, Wolfgang, Jian, Xueqiu, Pijnenburg, Yolande A L, Andreoni, Simona, Ferrarese, Carlo, Sala Gessica, Zoia, Chiara Paola, Kehoe, Patrick Gavin, Garcia-Ribas, Guillermo, Sánchez-Juan, Pascual, Pastor, Pau, Pérez-Tur, Jordi, Piñol-Ripoll, Gerard, Lopez de Munain, Adolfo, García-Alberca, Jose María, Bullido, María J, Álvarez, Victoria, Lleó, Alberto, Real, Luis M, Mir, Pablo, Medina, Miguel, Scheltens, Philip, Holstege, Henne, Marquié, Marta, Sáez, María Eugenia, Carracedo, Ángel, Amouyel, Philippe, Schellenberg, Gerard D, Williams, Julie, Seshadri, Sudha, van Duijn, Cornelia M, Mather, Karen A, Sánchez-Valle, Raquel, Serrano-Ríos, Manuel, Orellana, Adelina, Tárraga, Lluís, Blennow, Kaj, Huisman, Martijn, Andreassen, Ole A, Posthuma, Danielle, Clarimón, Jordi, Boada, Mercè, van der Flier, Wiesje M, Ramirez, Alfredo, Lambert, Jean-Charles, van der Lee, Sven J, and Ruiz, Agustín

Abstract

Genetic discoveries of Alzheimer’s disease are the drivers of our understanding, and together with polygenetic risk stratification can contribute towards planning of feasible and efficient preventive and curative clinical trials. We first perform a large genetic association study by merging all available case-control datasets and by-proxy study results (discovery n = 409,435 and validation size n = 58,190). Here, we add six variants associated with Alzheimer’s disease risk (near APP, CHRNE, PRKD3/NDUFAF7, PLCG2 and two exonic variants in the SHARPIN gene). Assessment of the polygenic risk score and stratifying by APOE reveal a 4 to 5.5 years difference in median age at onset of Alzheimer’s disease patients in APOE ɛ4 carriers. Because of this study, the underlying mechanisms of APP can be studied to refine the amyloid cascade and the polygenic risk score provides a tool to select individuals at high risk of Alzheimer’s disease.

Published
2021

12. Multicentre translational Trial of Remote Ischaemic Conditioning in Acute Ischaemic Stroke (TRICS): protocol of multicentre, parallel group, randomised, preclinical trial in female and male rat and mouse from the Italian Stroke Organization (ISO) Basic Science network

Author

Tettamanti, M, Beretta, S, Pignataro, G, Fumagalli, S, Perego, C, Sironi, L, Pedata, F, Amantea, D, Bacigaluppi, M, Vinciguerra, A, Valente, A, Diamanti, S, Mariani, J, Viganò, M, Santangelo, F, Zoia, C, Rogriguez-Menendez, V, Castiglioni, L, Rzemieniec, J, Dettori, I, Bulli, I, Coppi, E, Gullotta, G, Bagetta, G, Martino, G, Ferrarese, C, De Simoni, M, Tettamanti, Mauro, Beretta, Simone, Pignataro, Giuseppe, Fumagalli, Stefano, Perego, Carlo, Sironi, Luigi, Pedata, Felicita, Amantea, Diana, Bacigaluppi, Marco, Vinciguerra, Antonio, Valente, Alessia, Diamanti, Susanna, Mariani, Jacopo, Viganò, Martina, Santangelo, Francesco, Zoia, Chiara Paola, Rogriguez-Menendez, Virginia, Castiglioni, Laura, Rzemieniec, Joanna, Dettori, Ilaria, Bulli, Irene, Coppi, Elisabetta, Gullotta, Giorgia Serena, Bagetta, Giacinto, Martino, Gianvito, Ferrarese, Carlo, De Simoni, Maria Grazia, Tettamanti, M, Beretta, S, Pignataro, G, Fumagalli, S, Perego, C, Sironi, L, Pedata, F, Amantea, D, Bacigaluppi, M, Vinciguerra, A, Valente, A, Diamanti, S, Mariani, J, Viganò, M, Santangelo, F, Zoia, C, Rogriguez-Menendez, V, Castiglioni, L, Rzemieniec, J, Dettori, I, Bulli, I, Coppi, E, Gullotta, G, Bagetta, G, Martino, G, Ferrarese, C, De Simoni, M, Tettamanti, Mauro, Beretta, Simone, Pignataro, Giuseppe, Fumagalli, Stefano, Perego, Carlo, Sironi, Luigi, Pedata, Felicita, Amantea, Diana, Bacigaluppi, Marco, Vinciguerra, Antonio, Valente, Alessia, Diamanti, Susanna, Mariani, Jacopo, Viganò, Martina, Santangelo, Francesco, Zoia, Chiara Paola, Rogriguez-Menendez, Virginia, Castiglioni, Laura, Rzemieniec, Joanna, Dettori, Ilaria, Bulli, Irene, Coppi, Elisabetta, Gullotta, Giorgia Serena, Bagetta, Giacinto, Martino, Gianvito, Ferrarese, Carlo, and De Simoni, Maria Grazia

Abstract

Introduction Multicentre preclinical randomised controlled trials (pRCT) are emerging as a necessary step to confirm efficacy and improve translation into the clinic. The aim of this project is to perform two multicentre pRCTs (one in rats and one in mice) to investigate the efficacy of remote ischaemic conditioning (RIC) in an experimental model of severe ischaemic stroke. Methods and analysis Seven research laboratories within the Italian Stroke Organization (ISO) Basic Science network will participate in the study. Transient endovascular occlusion of the proximal right middle cerebral artery will be performed in two species (rats and mice) and in both sexes. Animals will be randomised to receive RIC by transient surgical occlusion of the right femoral artery, or sham surgery, after reperfusion. Blinded outcome assessment will be performed for dichotomised functional neuroscore (primary endpoint) and infarct volume (secondary endpoint) at 48 hours. A sample size of 80 animals per species will yield 82% power to detect a significant difference of 30% in the primary outcome in both pRCTs. Analyses will be performed in a blind status and according to an intention-to-treat paradigm. The results of this study will provide robust, translationally oriented, high-quality evidence on the efficacy of RIC in multiple species of rodents with large ischaemic stroke. Ethics and dissemination This is approved by the Animal Welfare Regulatory Body of the University of Milano Bicocca, under project license from the Italian Ministry of Health. Trial results will be subject to publication according to the definition of the outcome presented in this protocol.

Published
2020

13. Multicentre translational Trial of Remote Ischaemic Conditioning in Acute Ischaemic Stroke (TRICS): protocol of multicentre, parallel group, randomised, preclinical trial in female and male rat and mouse from the Italian Stroke Organization (ISO) Basic Science networkMulticentre translational Trial of Remote Ischaemic Conditioning in Acute Ischaemic Stroke (TRICS): protocol of multicentre, parallel group, randomised, preclinical trial in female and male rat and mouse from the Italian Stroke Organization (ISO) Basic Science network

Author

Tettamanti, Mauro, primary, Beretta, Simone, additional, Pignataro, Giuseppe, additional, Fumagalli, Stefano, additional, Perego, Carlo, additional, Sironi, Luigi, additional, Pedata, Felicita, additional, Amantea, Diana, additional, Bacigaluppi, Marco, additional, Vinciguerra, Antonio, additional, Valente, Alessia, additional, Diamanti, Susanna, additional, Mariani, Jacopo, additional, Viganò, Martina, additional, Santangelo, Francesco, additional, Zoia, Chiara Paola, additional, Rogriguez-Menendez, Virginia, additional, Castiglioni, Laura, additional, Rzemieniec, Joanna, additional, Dettori, Ilaria, additional, Bulli, Irene, additional, Coppi, Elisabetta, additional, Gullotta, Giorgia Serena, additional, Bagetta, Giacinto, additional, Martino, Gianvito, additional, Ferrarese, Carlo, additional, and De Simoni, Maria Grazia, additional

Published
2020
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14. Modulation of RAS and PI3-AKT pathway by Stavudine (d4T) in PBMC of Alzheimer’s Disease Patients

Author

Rosa, Francesca La, primary, Zoia, Chiara Paola, additional, Bazzini, Chiara, additional, Bolognini, Alessandra, additional, Saresella, Marina, additional, Conti, Elisa, additional, Ferrarese, Carlo, additional, Piancone, Federica, additional, Marventano, Ivana, additional, Galimberti, Daniela, additional, Fenoglio, Chiara, additional, Scarpini, Elio, additional, and Clerici, Mario, additional

Published
2020
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17. Oxidative stress and altered mitochondrial dynamics in neurodegenerative processes

Author

Formenti, M, Aprea, F, SALA, GESSICA, ZOIA, CHIARA PAOLA, RIVA, CHIARA, MARINIG, DANIELE, MARTORANA, FRANCESCA, SALA, BARBARA, Buglione, E, Kolodkin, A, Savarese, L, Balling, R, Papa, M, ALBERGHINA, LILIA, FERRARESE, CARLO, COLANGELO, ANNA MARIA, Formenti, M, Aprea, F, Sala, G, Zoia, C, Riva, C, Marinig, D, Martorana, F, Sala, B, Buglione, E, Kolodkin, A, Savarese, L, Balling, R, Papa, M, Alberghina, L, Ferrarese, C, and Colangelo, A

Subjects
oxidative stre, fusion, mitochondrial biogenesis, mitochondrial dynamic, neurodegeneration, fission
Abstract

Aims: Mitochondria are very dynamic organelles. Mitochondrial dynamics (alternation of fission/fusion cycles and their axonal transport) and biogenesis are crucial for neuronal homeostasis and synaptic function; moreover, alteration of the balance between fission and fusion can determine the fate of neuronal cells. Fission processes require the intervention of Fis1 and the active form of Drp1, whereas fusion is primarily regulated by Opa1 and Mfn1/2. We here focused on correlation between oxidative stress and mitochondrial dynamics and biogenesis. Materials and Methods: We compared the effect of hydrogen peroxide and Rotenone (a neurotoxin that inhibits the Complex-I of electrons transport chain), on primary cortical neurons or models of neurodegeneration based on neuroblastoma or PC12 cells. Protein levels and distribution (cytoplasm, mitochondria or nuclei) were assessed by western blots and immunofluorescence imaging. Results: Exposure of neurons or neuroblastoma to Rotenone determined a dose-dependent (200-400-800 nM) decrease of P-Drp1 and a biphasic trend of Opa1 and Mfn2 in a time-dependent manner (1-3-6-24h). In PC12 cells, alterations of mitochondrial proteins in response to hydrogen peroxide was paralleled by differential distribution (cytoplasm/nuclei) of oxidative-stress sensors (DJ-1, Nrf2) and proteins regulating mitochondrial biogenesis (mtTFAM and PGC1a), while determining a dose- and time-dependent increase of ROS and ATP depletion. Interestingly, human fibroblasts showed similar changes in response to rotenone but a different trend in response to Amyloid-beta oligomers. Discussion/Conclusions: These studies suggest 1) the existence of a complex cross-talk underlying mitochondrial dynamics and biogenesis, 2) alteration of mitochondrial dynamic proteins as biomarkers of mitochondrial dysfunction in neurodegenerative processes.

Published
2016

19. THE TOXICITY OF AMYLOID-BETA OLIGOMERS AND TAU HYPERPHOSPHORYLATION ARE PREVENTED FROM LIPID-BASED NANOPARTICLES IN HUMAN PRIMARY FIBOBLASTS

Author

ZOIA, CHIARA PAOLA, CONTI, ELISA, CEREDA, DILETTA, RODRIGUEZ MENENDEZ, VIRGINIA, ANDREONI, SIMONA, GREGORI, MARIA, SESANA, MARIA SILVIA, MASSERINI, MASSIMO ERNESTO, TREMOLIZZO, LUCIO, FERRARESE, CARLO, Zoia, C, Conti, E, Cereda, D, RODRIGUEZ MENENDEZ, V, Andreoni, S, Gregori, M, Sesana, M, Masserini, M, Tremolizzo, L, and Ferrarese, C

Subjects
nanoparticples, therapy for AD, peripheral model, oxidative stress
Abstract

Objective: Abeta leads to neurodegeneration and memory impairment via critical signal transduction processes, both at central and peripheral level. Studying the oligomeric-Abeta toxicity could helpful to clarify these phenomena, and to develop therapeutic and effective prevention technologies. Nanoparticles (NPs) biocompatibility and their ability to modulate Abeta toxicity were investigated in primary cultures from human fibroblasts. Methods: Control fibroblasts were incubated with Abeta oligomers and NP, functionalized or not with a ApoE monomer (mApo). By MTT assay we evaluate energetic metabolism and cell viability. Western Blot and p-Elisa were performed to evidence any modulation of MAPK and Tau hyperphosphorylation. By uptake studies and confocal images, we investigated the involvement of NPs in modulating the Abeta-induced excitotoxicity and their interaction with oligomers. By commercial kit, malondialdehyde levels were tested. Results: NPs do not affect cell viability, they prevent the Abeta-induced deficit of mitochondrial activity (p

Published
2016

21. Modulation of mapk phosphorylation in fibroblasts from alzheimer patients: erk involvment in glutamate transport and tau hyperphosporylation

Author

ZOIA, CHIARA PAOLA, CEREDA, DILETTA, CONTI, ELISA, TREMOLIZZO, LUCIO, ISELLA, VALERIA, FERRARESE, CARLO, Compagnoni, P, Ulisse, A, Verga, E, Zoia, C, Compagnoni, P, Ulisse, A, Verga, E, Cereda, D, Conti, E, Tremolizzo, L, Isella, V, and Ferrarese, C

Subjects
ERK, SAPKinase, TAU, Alzheimer, glutamate transporter
Abstract

Amyloid-beta (Abeta) affects cellular functions leading to neurodegeneration and memory impairment via critical signal transduction processes, including also the ERK1/2 pathway. We used fibroblasts from Alzheimer’s disease (AD) patients and age-related controls to investigate possible alterations of MAPK-pathway associated to the various stages of disease that might be helpful to clarify any molecular mechanisms involved in this systemic pathology. Phosphorylated p38- and JNK-MAPK were observed in fibroblasts from MCI (mild cognitive impairment) subjects, AD patients and in oligomeric-Abeta treated cells from healthy subjects, without any correlation with disease severity. On the other hand, ERK1/2 phosphorylation was reduced in fibroblasts from mild and moderate AD, compared to severe AD patients. Also in Abeta-treated fibroblasts from control subjects, a similar decrease was shown. In MCI subjects, a trend to phospho-ERK1/2 decrease was observed too. A specific correlation was demonstrated between ERK1/2 phosphorylation and disease severity.When the four converter-MCI cases were considered, the statistical significance is improved and correlation is confirmed. Moreover, ERK phosphorylation has been involved in hyper-phosphorylation of tau, which is evident before deposition of Abeta fibrils, through the activation of p70S6-kinase. In addition, we show that the ERK pathway is specifically involved in EAAT1 molecular translation, modulating its promoter and thus impairing glutamate transport, as described in fibroblasts from AD patients. These results suggest that the ERK signaling could be of great importance for the study of the pathogenesis and the disease severity, and to investigate new treatments in AD

Published
2015

22. Lipid-based nanoparticles counteract the oligomer abeta toxicity and tau phosphorylation in an ex-vivo biomolecular model of alzheimer disease

Author

ZOIA, CHIARA PAOLA, CONTI, ELISA, RODRIGUEZ MENENDEZ, VIRGINIA, GREGORI, MARIA, SESANA, MARIA SILVIA, MASSERINI, MASSIMO ERNESTO, FERRARESE, CARLO, Ulisse, A, Zoia, C, Conti, E, Ulisse, A, RODRIGUEZ MENENDEZ, V, Gregori, M, Sesana, M, Masserini, M, and Ferrarese, C

Subjects
Alzheimer, Nanoparticles, ERK, TAU , Abeta, primary fibroblats
Published
2015

23. Behavioural Anomalies in Neurodegenerative Diseases: Role of the Immune Response

Author

GRANA, DENISE, TREMOLIZZO, LUCIO, CONTI, ELISA, ZOIA, CHIARA PAOLA, APPOLLONIO, ILDEBRANDO, FERRARESE, CARLO, Grana, D, Tremolizzo, L, Conti, E, Zoia, C, Appollonio, I, and Ferrarese, C

Subjects
MED/26 - NEUROLOGIA, Alzheimer’s Disease (AD), Behavioural and Psychological Symptoms of Dementia (BPSD), Translocator protein 18-kDa (TSPO-18), neuroinflammation, chemotaxis, neurosteroids
Published
2015

24. THE TOXICITY OF AMYLOID-BETA OLIGOMERS AND TAU HYPERPHOSPHORYLATION ARE PREVENTED FROM LIPID-BASED NANOPARTICLES IN HUMAN PRIMARY FIBOBLASTS

Author

Zoia, C, Conti, E, Cereda, D, RODRIGUEZ MENENDEZ, V, Andreoni, S, Gregori, M, Sesana, M, Masserini, M, Tremolizzo, L, Ferrarese, C, ZOIA, CHIARA PAOLA, CONTI, ELISA, CEREDA, DILETTA, RODRIGUEZ MENENDEZ, VIRGINIA, ANDREONI, SIMONA, GREGORI, MARIA, SESANA, MARIA SILVIA, MASSERINI, MASSIMO ERNESTO, TREMOLIZZO, LUCIO, FERRARESE, CARLO, Zoia, C, Conti, E, Cereda, D, RODRIGUEZ MENENDEZ, V, Andreoni, S, Gregori, M, Sesana, M, Masserini, M, Tremolizzo, L, Ferrarese, C, ZOIA, CHIARA PAOLA, CONTI, ELISA, CEREDA, DILETTA, RODRIGUEZ MENENDEZ, VIRGINIA, ANDREONI, SIMONA, GREGORI, MARIA, SESANA, MARIA SILVIA, MASSERINI, MASSIMO ERNESTO, TREMOLIZZO, LUCIO, and FERRARESE, CARLO

Abstract

Objective: Abeta leads to neurodegeneration and memory impairment via critical signal transduction processes, both at central and peripheral level. Studying the oligomeric-Abeta toxicity could helpful to clarify these phenomena, and to develop therapeutic and effective prevention technologies. Nanoparticles (NPs) biocompatibility and their ability to modulate Abeta toxicity were investigated in primary cultures from human fibroblasts. Methods: Control fibroblasts were incubated with Abeta oligomers and NP, functionalized or not with a ApoE monomer (mApo). By MTT assay we evaluate energetic metabolism and cell viability. Western Blot and p-Elisa were performed to evidence any modulation of MAPK and Tau hyperphosphorylation. By uptake studies and confocal images, we investigated the involvement of NPs in modulating the Abeta-induced excitotoxicity and their interaction with oligomers. By commercial kit, malondialdehyde levels were tested. Results: NPs do not affect cell viability, they prevent the Abeta-induced deficit of mitochondrial activity (p<0.001) and MAPK phosphorylation (p<0.001). mApo cross cell membrane, bind oligomers and modulate glutamate uptake in a dose dependent manner (p<0.01); they counteract the increase of Abeta–induced lipid peroxidation (p<0.002) and Tau-hyperphosphorylation (p<0.0001). Conclusion: These results suggest that NPs could represent a useful tool to antagonize Abeta oligomers toxicity, counteracting the cellular stress, protein epigenetic regulation and glutamate cytotoxicity. The modulations of MAPK pathways, altered by Abeta oligomers, might clarify any molecular mechanisms that mirror those involved in the early AD phases, and NPs may be useful to study new potential therapeutic strategies.

Published
2016

25. Peripheral sink effect as possible nanotherapeutic strategy for alzheimer’s disease: an ex vivo study

Author

Conti, E, Zoia, C, Grana, D, DA RE, F, Gregori, M, Re, F, Masserini, M, Ferrarese, C, Tremolizzo, L, CONTI, ELISA, ZOIA, CHIARA PAOLA, GRANA, DENISE, DA RE, FULVIO, GREGORI, MARIA, RE, FRANCESCA, MASSERINI, MASSIMO ERNESTO, FERRARESE, CARLO, TREMOLIZZO, LUCIO, Conti, E, Zoia, C, Grana, D, DA RE, F, Gregori, M, Re, F, Masserini, M, Ferrarese, C, Tremolizzo, L, CONTI, ELISA, ZOIA, CHIARA PAOLA, GRANA, DENISE, DA RE, FULVIO, GREGORI, MARIA, RE, FRANCESCA, MASSERINI, MASSIMO ERNESTO, FERRARESE, CARLO, and TREMOLIZZO, LUCIO

Published
2016

26. Donepezil modulates the endogenous immune response: implications for Alzheimer's disease

Author

Conti, Elisa, primary, Tremolizzo, Lucio, additional, Santarone, Marta Elena, additional, Tironi, Marco, additional, Radice, Isabella, additional, Zoia, Chiara Paola, additional, Aliprandi, Angelo, additional, Salmaggi, Andrea, additional, Dominici, Roberto, additional, Casati, Marco, additional, Appollonio, Ildebrando, additional, and Ferrarese, Carlo, additional

Published
2016
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27. Lipid-based nanoparticles counteract the oligomer abeta toxicity and tau phosphorylation in an ex-vivo biomolecular model of alzheimer disease

Author

Zoia, C, Conti, E, Ulisse, A, RODRIGUEZ MENENDEZ, V, Gregori, M, Sesana, M, Masserini, M, Ferrarese, C, ZOIA, CHIARA PAOLA, CONTI, ELISA, RODRIGUEZ MENENDEZ, VIRGINIA, GREGORI, MARIA, SESANA, MARIA SILVIA, MASSERINI, MASSIMO ERNESTO, FERRARESE, CARLO, Zoia, C, Conti, E, Ulisse, A, RODRIGUEZ MENENDEZ, V, Gregori, M, Sesana, M, Masserini, M, Ferrarese, C, ZOIA, CHIARA PAOLA, CONTI, ELISA, RODRIGUEZ MENENDEZ, VIRGINIA, GREGORI, MARIA, SESANA, MARIA SILVIA, MASSERINI, MASSIMO ERNESTO, and FERRARESE, CARLO

Published
2015

28. Modulation of mapk phosphorylation in fibroblasts from alzheimer patients: erk involvment in glutamate transport and tau hyperphosporylation

Author

Zoia, C, Compagnoni, P, Ulisse, A, Verga, E, Cereda, D, Conti, E, Tremolizzo, L, Isella, V, Ferrarese, C, ZOIA, CHIARA PAOLA, CEREDA, DILETTA, CONTI, ELISA, TREMOLIZZO, LUCIO, ISELLA, VALERIA, FERRARESE, CARLO, Zoia, C, Compagnoni, P, Ulisse, A, Verga, E, Cereda, D, Conti, E, Tremolizzo, L, Isella, V, Ferrarese, C, ZOIA, CHIARA PAOLA, CEREDA, DILETTA, CONTI, ELISA, TREMOLIZZO, LUCIO, ISELLA, VALERIA, and FERRARESE, CARLO

Abstract

Amyloid-beta (Abeta) affects cellular functions leading to neurodegeneration and memory impairment via critical signal transduction processes, including also the ERK1/2 pathway. We used fibroblasts from Alzheimer’s disease (AD) patients and age-related controls to investigate possible alterations of MAPK-pathway associated to the various stages of disease that might be helpful to clarify any molecular mechanisms involved in this systemic pathology. Phosphorylated p38- and JNK-MAPK were observed in fibroblasts from MCI (mild cognitive impairment) subjects, AD patients and in oligomeric-Abeta treated cells from healthy subjects, without any correlation with disease severity. On the other hand, ERK1/2 phosphorylation was reduced in fibroblasts from mild and moderate AD, compared to severe AD patients. Also in Abeta-treated fibroblasts from control subjects, a similar decrease was shown. In MCI subjects, a trend to phospho-ERK1/2 decrease was observed too. A specific correlation was demonstrated between ERK1/2 phosphorylation and disease severity.When the four converter-MCI cases were considered, the statistical significance is improved and correlation is confirmed. Moreover, ERK phosphorylation has been involved in hyper-phosphorylation of tau, which is evident before deposition of Abeta fibrils, through the activation of p70S6-kinase. In addition, we show that the ERK pathway is specifically involved in EAAT1 molecular translation, modulating its promoter and thus impairing glutamate transport, as described in fibroblasts from AD patients. These results suggest that the ERK signaling could be of great importance for the study of the pathogenesis and the disease severity, and to investigate new treatments in AD

Published
2015

29. Peripheral sink effect as possible nanotherapeutic strategy for alzheimer’s disease: an ex vivo study

Author

Conti, E, Zoia, C, Tremolizzo, L, Grana, D, Gregori, M, Re, F, Masserini, M, Ferrarese, C, CONTI, ELISA, ZOIA, CHIARA PAOLA, TREMOLIZZO, LUCIO, GRANA, DENISE, GREGORI, MARIA, RE, FRANCESCA, MASSERINI, MASSIMO ERNESTO, FERRARESE, CARLO, Conti, E, Zoia, C, Tremolizzo, L, Grana, D, Gregori, M, Re, F, Masserini, M, Ferrarese, C, CONTI, ELISA, ZOIA, CHIARA PAOLA, TREMOLIZZO, LUCIO, GRANA, DENISE, GREGORI, MARIA, RE, FRANCESCA, MASSERINI, MASSIMO ERNESTO, and FERRARESE, CARLO

Published
2015

30. Behavioural Anomalies in Neurodegenerative Diseases: Role of the Immune Response

Author

Grana, D, Tremolizzo, L, Conti, E, Zoia, C, Appollonio, I, Ferrarese, C, GRANA, DENISE, TREMOLIZZO, LUCIO, CONTI, ELISA, ZOIA, CHIARA PAOLA, APPOLLONIO, ILDEBRANDO, FERRARESE, CARLO, Grana, D, Tremolizzo, L, Conti, E, Zoia, C, Appollonio, I, Ferrarese, C, GRANA, DENISE, TREMOLIZZO, LUCIO, CONTI, ELISA, ZOIA, CHIARA PAOLA, APPOLLONIO, ILDEBRANDO, and FERRARESE, CARLO

Published
2015

31. MAPK phosphorylation in fibroblasts from Alzheimer's disease patients: ERK involvement in EAAT1 expression and modulation with disease severity

Author

ZOIA, CHIARA PAOLA, CONTI, ELISA, SUSANI, EMANUELA LAURA, COSTANTINO, GISELLA, CEREDA, DILETTA, ISELLA, VALERIA, TREMOLIZZO, LUCIO, FERRARESE, CARLO, Zoia, C, Conti, E, Susani, E, Costantino, G, Cereda, D, Isella, V, Tremolizzo, L, and Ferrarese, C

Subjects
MED/26 - NEUROLOGIA, MAPK phosphorylation, Alzheimer's disease, fibroblast
Published
2013

34. Longitudinal effects on plasma abeta levels of imatinib, a gamma-secretase activating protein inhibitor

Author

TREMOLIZZO, LUCIO, CONTI, ELISA, ZOIA, CHIARA PAOLA, GAMBACORTI PASSERINI, CARLO, FERRARESE, CARLO, Rizzo, C, Weksler, M, Weksler, B, Tremolizzo, L, Conti, E, Zoia, C, Rizzo, C, GAMBACORTI PASSERINI, C, Weksler, M, Weksler, B, and Ferrarese, C

Subjects
amyloid beta, imatinib
Abstract

Imatinib Mesylate is a competitive tyrosine-kinase inhibitor used in the treatment of multiple types of leukaemia, including Ph+ chronic myelogenous leukaemia (CML). The recent report that Imatinib inhibits gamma-secretase activating protein (gSAP), reducing beta-amyloid- (Abeta) production without affecting Notch-1 cleavage has led to the proposal of a possible application of this drug in AD therapeutics. In fact, one major drawback of Semagacestat, a direct inhibitor of gamma-secretase, was identified in the low Abeta/Notch therapeutic index, with the consequent failure of the phase III trial. Imatinib peripheral administration (20 mg/kg/day) was able to reduce Abeta content in mouse brain. Usually, leukaemia patients receive an average oral dose of 400 mg/day (∼5–6 mg/kg/day). In this longitudinal study we investigated if Imatinib administration in 10 leukemic patients could reduce plasma Abeta1–40 levels. Patients’ blood was sampled prior to treatment (time T0) and after 15, 30, and 60-to-90 days of treatment. Abeta1–40 plasma levels were assessed by ELISA as previously described. No significant reduction in plasma Abeta1–40 was observed at any time point. In fact, ratios to T0 of Abeta1–40 T15-, T30- and T90 were: 1.07 ± 0.39, 1.37 ± 0.59, and 1.48 ± 0.61, respectively, indicating no decrease with Imatinib. While these results are negative at standard dosing of Imatinib for CML, they do not exclude the possibility that Abeta lowering effects might be present at higher doses of this drug or with second generation tyrosine-kinase inhibitors, such as Dasatinib or Nilotinib, which can cross the blood brain barrier more easily. Further exploration of this issue might lead to advances in AD therapeutics

Published
2013

35. Donepezil modulates the endogenous immune response in Alzheimer’s disease, an ex vivo and in vitro study

Author

TREMOLIZZO, LUCIO, CONTI, ELISA, ZOIA, CHIARA PAOLA, APPOLLONIO, ILDEBRANDO, FERRARESE, CARLO, Santarone, M, Tironi, M, Tremolizzo, L, Conti, E, Santarone, M, Tironi, M, Zoia, C, Appollonio, I, and Ferrarese, C

Subjects
Donepezil, immune response, abeta
Abstract

Donepezil (DNPZ), an acetyl-cholinesterase inhibitor (AChEI), is widely used as a symptomatic drug in AD, given the involvement of cholinergic damage in the onset of memory loss. DNPZ, besides being able to improve acetylcholine (ACh) transmission, might have a neuroprotective effect reducing toxic Abeta fibrils and regulating the immune response. Considering that we previously showed a specific increase of plasma anti-Abeta1–42 antibodies in AD patients treated with AChEI, we evaluated in this study the role of DNPZ in favouring a Th2 phenotype, involved in modulating the immune humoral response. Moreover we hypothesized that this mechanism might be mediated by the a7-nicotinic ACh receptor expressed in lymphocytes. 60 patients with mild or moderate AD, either treated (n=22) or not (n=38) with DNPZ, and 30 controls were enrolled. AD DNPZ+ showed significantly higher plasma levels of anti-Abeta antibodies than DNPZ− (+40%) and lower levels of Abeta 1–42 than controls (−50%). In a subgroup of subjects, GATA-3, a transcription factor involved in Th2 differentiation, and a7nAChR expression was evaluated. No differences were found in GATA-3 mRNA in AD DNPZ+ when compared to DNPZ− and controls. On the opposite, we found by chromatine immunoprecipitation a significant three-fold increase of the association of GATA-3 with the IL-5-promoter in DNPZ+ patients, which also showed a significant twofold increase in a7-nAChR mRNA, with respect to DNPZ-ones. In vitro analyses demonstrated that the capacity of DNPZ to modulate GATA-3 expression is mediated by a7nAChR, since MLA, a specific antagonist, prevents it. Further studies are needed to better understand the role of DNPZ in modulating the immune response against Abeta, possibly ameliorating therapeutic strategies for AD

Published
2013

41. Glutamatergic Homeostasis Impairment in Multiple Sclerosis Patients

Author

Gironi, M, Vaghi, M, Mendozzi, L, Cavarretta, R, ZOIA, CHIARA PAOLA, FRIGO, MAURA, CAVALETTI, GUIDO ANGELO, FERRARESE, CARLO, Gironi, M, Zoia, C, Vaghi, M, Mendozzi, L, Cavarretta, R, Frigo, M, Cavaletti, G, and Ferrarese, C

Subjects
MED/26 - NEUROLOGIA, EAAT, disease severity, glutamate uptake
Abstract

Background: Background There is huge body of evidences concerning the detrimental role of glutamate excess in Multiple Sclerosis (MS). Excessive neuronal stimulation, due to deficient removal of glutamate and\or excessive release by activated Tcell and microglia, may trigger an enzymatic cascade leading to cell death. For MS, however, is still not cleared-up whether this imbalance relies to a glutamate overproduction or to its reduced clearance. Our group previously reported an impairment of glutamate transporter (EAAT) in neurodegenerative diseases at platelets (P) level, mirroring that present at central nervous system. Design/Methods: Design/Methods: We investigated P-EAAT activity as Glutamate-uptake marker, in primary progressive (PP=17), secondary (SP=16), relapsing remitting (RR=25) and benign (BB=12) MS population (=70) and 60 Healthy controls (HC). Sodium/energy-dependent-glutamate-uptake was studied in platelets measuring tritiated-Glu by beta-counter, following the original method of Mangano and Schwarcz, and activity and affinity were expressed by Vm and Km. Results: Results: Reduced glutamate-uptake values were found in MS compared to controls (18,23 1.82; 26.26 2.03 pmoles/mg prot/30min S.E.; p=0.03). Statistical analysis by one-way ANOVA showed p

Published
2012

43. Oxidative stress and glutamate uptake impairment in Multiple Sclerosis: any possible relationship?

Author

Gironi, M, ZOIA, CHIARA PAOLA, Mariani, E, Battifoglio, M, Cursano, C, Angelini, A, Stolfo, V, Rovaris, M, Saresella, M, Calabrese, E, Nemni, R, Tortorella, P, Grossi, E, Verga, E, Frigo, M, CAVALETTI, GUIDO ANGELO, FERRARESE, CARLO, Gironi, M, Zoia, C, Mariani, E, Battifoglio, M, Cursano, C, Angelini, A, Stolfo, V, Rovaris, M, Saresella, M, Calabrese, E, Nemni, R, Tortorella, P, Grossi, E, Verga, E, Frigo, M, Cavaletti, G, and Ferrarese, C

Subjects
MED/26 - NEUROLOGIA, oxidative stress, excitotoxicity, glutamate transportrs, disease severity
Abstract

Glutamate-uptake (GLU-up) impairment and an associated excitotoxic neuronal damage are widely documented in neurodegenerative diseases, as Multiple Sclerosis (MS) can be considered. Several factors negatively affect GLU-up, among them is the oxidative stress (OXS). Considering the suggested involvement of both GLU-up and OXS in neurodegenerative mechanisms of MS, we investigated a possible relationship between these factors. Fifty MS patients (age 33–64 years) with different disease course and 55 controls (HC, age 33–60 years) were studied for several serum markers of OXS and antioxidant factors, namely: coenzyme-Q10, total, oxidized and reduced forms of glutathione, malondialdehyde, reactive-oxygen-species, anti-oxidized-low-density lipoproteins antibodies (Anti-oxLDL), and anti-oxidant-power. Activity and affinity of glutamate platelet transporters were also studied. Coenzyme-Q10 and malondialdehyde were determined by an isocratic HPLC with UV detection, glutathione by HPLC with fluorescence detection, reactive-oxygen-species were photometrically quantified, anti-oxLDL were detected by ELISA, anti-oxidant-power was measured by the Cu++ reduction, subsequently detected by a chromogenic reaction. Sodium/energy-dependent-glutamate-uptake was studied in platelets measuring tritiated-Glu by b-counter and normalized to proteins’ amount. Reduced GLU-up values was found in MS compared to HC (19 ± 1.8; 28 ± 2.4 pmoles/mg prot/30 min ± SEM; p B 0.003), even if in benign MS subgroup no uptake decrease was observed. Coenzyme-Q10, one of the most powerful antioxidant was lower in MS (517 ± 35.7 lg/L) compared to HC (626 ± 27.7 lg/L p.02). While oxidated- glutathione levels were higher in SM (62 ± 3.7 U/ml) versus HC (48 ± 4.3U/ml, p = 0.01). A trend for higher antioxLDL in MS patients (35 ± 2.5 U/ml) versus HC (31 ± 4.7 U/ml) was found as well. We described a reduced GLU-up activity in MS patients compared to HC; Vm was significantly decreased (p.003), whereas Km was unaffected. Hence, we might suggest that the uptake impairment is associated to a reduced GLU- transporter expression or to a structural damage. OXS maybe one of the mechanisms involved in both these processes. Interestingly, we found a higher antioxidant ‘‘protection’’ in benign MS, where GLU-up was increased versus RR and SP SM (p.05). Correlation analyses between all OXS markers measured and GLU-up as well as across different courses of MS patients will be discussed

Published
2011

46. Novel therapeutic targets in neuropsychiatric disorders: the neuroepigenome

Author

Tremolizzo, L, RODRIGUEZ MENENDEZ, V, Conti, E, Zoia, C, Cavaletti, G, Ferrarese, C, TREMOLIZZO, LUCIO, RODRIGUEZ MENENDEZ, VIRGINIA, CONTI, ELISA, ZOIA, CHIARA PAOLA, CAVALETTI, GUIDO ANGELO, FERRARESE, CARLO, Tremolizzo, L, RODRIGUEZ MENENDEZ, V, Conti, E, Zoia, C, Cavaletti, G, Ferrarese, C, TREMOLIZZO, LUCIO, RODRIGUEZ MENENDEZ, VIRGINIA, CONTI, ELISA, ZOIA, CHIARA PAOLA, CAVALETTI, GUIDO ANGELO, and FERRARESE, CARLO

Abstract

The neuroepigenome, i.e., the epigenome of the nervous system, has become interesting for therapeutics in the last years due to widespread availability of dedicated drugs. A pivotal role for neuroepigenetics is certainly implied, both in physiology and pathology, by the highly dynamic structural and functional rearrangements that constantly occur into the nervous system, globally known as plasticity. Moreover, the idea that the pathophysiology of several neuropsychiatric disorders might involve epigenetic mechanisms is increasingly taking place due to accumulating experimental data and by the evidence of a synergistic interaction between genes and environment beneath most sporadic forms of these diseases. In this paper we will review the available evidence on the use of epigenome-modifying drugs in the field of neuropsychiatry, shortly describing for each disease the underlying assumptions of an epigenetic dysregulation. © 2014 Bentham Science Publishers.

Published
2014

49. Imunohystochemical characterization of the distribution of glutamate receptors, 'high affinity' membrane bound glutamate transporters and of Glutamate Carboxipeptidase II (GCPII) in in vitro cultures of embryonic dorsal root ganglia

Author

CAROZZI, VALENTINA ALDA, ZOIA, CHIARA PAOLA, MARMIROLI, PAOLA LORENA, FERRARESE, CARLO, CAVALETTI, GUIDO ANGELO, TREDICI, GIOVANNI, Carozzi, V, Zoia, C, Marmiroli, P, Ferrarese, C, Cavaletti, G, and Tredici, G

Subjects
glutamate, sensory neurons, peripheral nervous system
Published
2009

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