33 results on '"Zoi, Vasiliki"'
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2. Antitumor activity of 5-hydroxy-3′,4′,6,7-tetramethoxyflavone in glioblastoma cell lines and its antagonism with radiotherapy
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Papapetrou Panagiota, Dimitriadis Kyriakos, Galani Vasiliki, Zoi Vasiliki, Giannakopoulou Maria, Papathanasopoulou Vasiliki A., Sioka Chrissa, Tsekeris Pericles, Kyritsis Athanassios P., Lazari Diamanto, and Alexiou George A.
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5-hydroxy-3′,4′,6,7-tetramethoxyflavone ,glioblastoma ,radiotherapy ,Biology (General) ,QH301-705.5 - Abstract
5-Hydroxy-3′,4′,6,7-tetramethoxyflavone (TMF) is a plant-origin flavone known for its anti-cancer properties. In the present study, the cytotoxic effect of TMF was evaluated in the U87MG and T98G glioblastoma (GBM) cell lines. The effect of TMF on cell viability was assessed with trypan blue exclusion assay and crystal violet staining. In addition, flow cytometry was performed to examine its effect on the different phases of the cell cycle, and in vitro scratch wound assay assessed the migratory capacity of the treated cells. Furthermore, the effect of in vitro radiotherapy was also evaluated with a combination of TMF and radiation. In both cell lines, TMF treatment resulted in G0/G1 cell cycle arrest, reduced cell viability, and reduced cell migratory capacity. In contrast, there was an antagonistic property of TMF treatment with radiotherapy. These results demonstrated the antineoplastic effect of TMF in GBM cells in vitro, but the antagonistic effect with radiotherapy indicated that TMF should be further evaluated for its possible antitumor role post-radiotherapy.
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- 2024
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3. Natural Compounds in Glioma Therapy
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Zoi, Vasiliki, primary, Giannakopoulou, Maria, additional, Alexiou, George A., additional, Kyritsis, Athanassios P., additional, and Sioka, Chrissa, additional
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- 2023
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4. Design Principles and Applications of Fluorescent Kinase Inhibitors for Simultaneous Cancer Bioimaging and Therapy.
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Ganai, Ab Majeed, Vrettos, Eirinaios I., Kyrkou, Stavroula G., Zoi, Vasiliki, Khan Pathan, Tabasum, Karpoormath, Rajshekhar, Bouziotis, Penelope, Alexiou, George A., Kastis, George A., Protonotarios, Nicholas E., and Tzakos, Andreas G.
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THERAPEUTIC use of antineoplastic agents ,PROTEIN kinase inhibitors ,FLUORESCENT dyes ,DIAGNOSTIC imaging ,MOLECULAR structure ,DRUG efficacy ,TUMORS ,INDIVIDUALIZED medicine - Abstract
Simple Summary: This review highlights the recent advances in the development and application of dual function kinase inhibitors that also bear fluorescent properties (fluorescent kinase inhibitors), thus can be used as theranostics in the field of cancer. This is a rapidly growing field with significant potential for cancer therapy and diagnosis. This work mainly focuses on the key design principles that guide the development of these multifunctional compounds, emphasizing the integration of essential components such as the kinase cytotoxic warhead, the fluorophore, the linkers, and additional modular elements to enhance the efficacy of the final assembled compound. We anticipate this review to propel the advancement of this field by improving the understanding of the design principles and ultimately leading to the development of more effective tools for the concurrent diagnosis and treatment of cancer. Kinase inhibitors are potent therapeutic agents in cancer treatment, but their effectiveness is frequently restricted by the inability to image the tumor microenvironment. To address this constraint, kinase inhibitor–fluorophore conjugates have emerged as promising theranostic agents, allowing for simultaneous cancer diagnosis and treatment. These conjugates are gaining attention for their ability to visualize malignant tissues and concurrently enhance therapeutic interventions. This review explores the design principles governing the development of multimodal inhibitors, highlighting their potential as platforms for kinase tracking and inhibition via bioimaging. The structural aspects of constructing such theranostic agents are critically analyzed. This work could shed light on this intriguing field and provide adequate impetus for developing novel theranostic compounds based on small molecule inhibitors and fluorophores. [ABSTRACT FROM AUTHOR]
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- 2024
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5. A Novel Fluorescent Gemcitabine Prodrug That Follows a Nucleoside Transporter‐Independent Internalization and Bears Enhanced Therapeutic Efficacy With Respect to Gemcitabine.
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Vrettos, Eirinaios Ι., Kyrkou, Stavroula G., Zoi, Vasiliki, Giannakopoulou, Maria, Chatziathanasiadou, Maria V., Kanaki, Zoi, Agalou, Adamantia, Bistas, Vasileios‐Panagiotis, Kougioumtzi, Anastasia, Karampelas, Theodoros, Diamantis, Dimitrios A., Murphy, Carol, Beis, Dimitris, Klinakis, Apostolos, Tamvakopoulos, Constantin, Kyritsis, Athanasios P., Alexiou, George A., and Tzakos, Andreas G.
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NUCLEOSIDE transport proteins ,DRUG monitoring ,FLUORESCENT dyes ,CONFOCAL microscopy ,ANTINEOPLASTIC agents ,ENDOCYTOSIS ,BLOOD plasma - Abstract
The multiplexity of cancer has rendered it the second leading cause of mortality worldwide and theragnostic prodrugs have gained popularity in recent years as a means of treatment. Theragnostic prodrugs enable the simultaneous diagnosis and therapy of tumors via high‐precision real‐time drug release monitoring. Herein, we report the development of the small theragnostic prodrug GF, based on the nucleoside anticancer agent gemcitabine and the fluorescent dye 5(6)‐carboxyfluorescein. We have successfully demonstrated its efficient internalization in tumor cells, showing localization throughout both the early and late endocytic pathways. Its mechanism of cell internalization was evaluated, confirming its independence from nucleoside transporters. Its cellular localization via confocal microscopy revealed a clathrin‐mediated endocytosis mechanism, distinguishing it from analogous compounds studied previously. Furthermore, GF exhibited stability across various pH values and in human blood plasma. Subsequently, its in vitro cytotoxicity was assessed in three human cancer cell lines (A549, U87 and T98). Additionally, its pharmacokinetic profile in mice was investigated and the consequent drug release was monitored. Finally, its in vivo visualization was accomplished in zebrafish xenotransplantation models and its in vivo efficacy was evaluated in A549 xenografts. The results unveiled an intriguing efficacy profile, positioning GF as a compelling candidate warranting further investigation. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Antineoplastic Activity of 9″-Lithospermic Acid Methyl Ester in Glioblastoma Cells
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Tzitiridou, Panagiota, primary, Zoi, Vasiliki, additional, Papagrigoriou, Theodora, additional, Lazari, Diamanto, additional, Sioka, Chrissa, additional, Alexiou, Georgios A., additional, and Kyritsis, Athanassios P., additional
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- 2024
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7. Nuclear Medicine and Cancer Theragnostics: Basic Concepts
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Zoi, Vasiliki, primary, Giannakopoulou, Maria, additional, Alexiou, George A., additional, Bouziotis, Pinelopi, additional, Thalasselis, Savvas, additional, Tzakos, Andreas G., additional, Fotopoulos, Andreas, additional, Papadopoulos, Athanassios N., additional, Kyritsis, Athanassios P., additional, and Sioka, Chrissa, additional
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- 2023
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8. Nuclear Medicine and Cancer Theragnostics
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Zoi, Vasiliki, primary, Giannakopoulou, Maria, additional, Alexiou, George A, additional, Bouziotis, Pinelopi, additional, Thalasselis, Savvas, additional, Tzakos, Andreas G, additional, Fotopoulos, Andreas, additional, Kyritsis, Athanassios P, additional, and Sioka, Chrissa, additional
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- 2023
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9. The Role of Curcumin in Cancer: A Focus on the PI3K/Akt Pathway.
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Zoi, Vasiliki, Kyritsis, Athanassios P., Galani, Vasiliki, Lazari, Diamanto, Sioka, Chrissa, Voulgaris, Spyridon, and Alexiou, Georgios A.
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PROTEIN kinases , *CANCER invasiveness , *GLIOMAS , *CELL proliferation , *BREAST tumors , *HEAD & neck cancer , *CELLULAR signal transduction , *PROSTATE tumors , *METASTASIS , *CELL lines , *CURCUMIN , *MOLECULAR structure , *GROWTH factors , *TUMORS , *PHOSPHOTRANSFERASES , *PATHOLOGIC neovascularization , *CELL survival , *CYTOKINES , *DISEASE progression - Abstract
Simple Summary: Cancer has a major impact on societies across the world. In an attempt to find new treatment options for cancer, attention has shifted to natural compounds. Curcumin is a polyphenol isolated from the roots of turmeric that possesses many biological properties. It acts on the regulation of different aspects of tumor development and interconnects with major signaling pathways that are dysregulated in cancer, such as the phosphatidylinositol-3-kinase/protein kinase B pathway. In this review, the diverse effects of curcumin on the regulation of this pathway in different malignancies will be discussed. Cancer is a life-threatening disease and one of the leading causes of death worldwide. Despite significant advancements in therapeutic options, most available anti-cancer agents have limited efficacy. In this context, natural compounds with diverse chemical structures have been investigated for their multimodal anti-cancer properties. Curcumin is a polyphenol isolated from the rhizomes of Curcuma longa and has been widely studied for its anti-inflammatory, anti-oxidant, and anti-cancer effects. Curcumin acts on the regulation of different aspects of cancer development, including initiation, metastasis, angiogenesis, and progression. The phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) pathway is a key target in cancer therapy, since it is implicated in initiation, proliferation, and cancer cell survival. Curcumin has been found to inhibit the PI3K/Akt pathway in tumor cells, primarily via the regulation of different key mediators, including growth factors, protein kinases, and cytokines. This review presents the therapeutic potential of curcumin in different malignancies, such as glioblastoma, prostate and breast cancer, and head and neck cancers, through the targeting of the PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Association of Melatonin Administration in Pregnant Ewes with Growth, Redox Status and Immunity of Their Offspring
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Efterpi Bouroutzika, Maria Giovanna Ciliberti, Mariangela Caroprese, Ekaterini Theodosiadou, Serafeim Papadopoulos, Sotiria Makri, Zoi-Vasiliki Skaperda, Georgios Kotsadam, Marios-Lazaros Michailidis, George Valiakos, Stella Chadio, Dimitris Kouretas, and Irene Valasi
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melatonin ,antioxidant biomarkers ,cytokines ,IgG ,lambs ,prenatal stress ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
In this study, the effects of melatonin treatment on growth, redox status and immunity in prenatally stressed newborn lambs were evaluated. Thirty-seven newborn lambs were allocated into two groups (melatonin-MEL and control-CON), based on whether their mothers were treated with melatonin implants or not, respectively. All pregnant ewes were exposed to heat stress. The body weight of lambs was recorded at birth (L0), and then on days 15 (L15) and 40 (L40). Redox biomarkers [total antioxidant capacity (TAC), glutathione (GSH), thiobarbituric acid reactive substances (TBARS)] were assayed in blood samples collected from lambs on days L0, L1, L2, L5, L10 and L40. Chemical analysis and antioxidant capacity were evaluated in colostrum and milk samples collected at the same time points with blood samples. Cytokines (IL-1β, IL-6, IL-10, IFN-γ) and immunoglobulin (IgG) were assayed in blood and colostrum samples collected from ewes on days L0 and L1, and in lambs’ blood on days L0, L1 and L2. The results revealed that body weight gain of newborn lambs did not differ between the two groups (p > 0.05). Better redox status was found in MEL lambs until L2, as well as higher antioxidant capacity in the colostrum of MEL ewes compared to CON ones on day L0 (p < 0.05). In MEL ewes’ colostrum, higher protein content was measured on day L0 and higher fat content on L1 compared to CON group (p < 0.05). The highest level of IL-6 was found in MEL ewes on L1, with a concomitant increase of IL-10 level in MEL lambs in comparison to CON lambs on L2. Moreover, CON colostrum resulted in a higher level of IL-10 within time, coupled with an increased level of IgG found in lambs’ plasma on L2 (p = 0.04). This study indicated that melatonin could be administered as antioxidant and immune-modulatory regime in prenatally stressed offspring in order to cope with the crucial first days of their life. This effect of melatonin was also amplified by crosstalk between IL-6, IL-10 and IgG production, resulting in an improved quality of produced milk.
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- 2021
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11. Μελέτη της αντικαρκινικής δράσης και του μηχανισμού της κουρκουμίνης στο γλοιοβλάστωμα
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Zoi, Vasiliki, Γαλάνη, Βασιλική, Βούλγαρης, Σπυρίδων, Αλεξίου, Γεώργιος, Τσέκερης, Περικλής, Βούλγαρη, Παρασκευή, Κυρίτσης, Αθανάσιος, and Ζήκου, Αναστασία
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Χημειο-Ακτινοθεραπεία ,Curcumin ,Mechanism of anticancer effect ,Κουρκουμίνη ,Μηχανισμός αντικαρκινικής δράσης ,Γλοιοβλάστωμα ,Oncology-Glioblastoma treatment ,Chemo-Radiotherapy ,Glioblastoma ,Ογκολογία-Θεραπεία Γλοιοβαστώματος - Abstract
Το γλοιοβλάστωμα είναι ένας από τους πιο θανατηφόρους όγκους του κενρικού νευρικού συστήματος, με μέση επιβίωση των ασθενών περίπου 15 μήνες μετά τη διάγνωση. Οι τρέχουσες θεραπευτικές επιλογές που βασίζονται στην χειρουργική εκτομή, ακολουθούμενη από συνδυασμό χημειοθεραπείας και ακτινοθεραπείας δεν αυξάνουν σημαντικά την επιβίωση των ασθενών. Για το λόγο αυτό, η ανάγκη εύρεσης νέων ασφαλών και αποτελεσματικών αντιγλοιωματικών παραγόντων κρίνεται απαραίτητη. Στο πλαίσιο αυτό, πολλές σύγχρονες ερευνητικές προσπάθειες έχουν στραφεί στην μελέτη φυτικών εκχυλισμάτων ή μορίων που απομονώνονται από φυτά. Η κουρκουμίνη είναι μια πολυφαινόλη που απομονώνεται από το φυτό Curcuma longa και έχει επιδείξει σημαντικές αντικαρκινικές ιδιότητες σε διάφορους τύπους νεοπλασιών. Στην παρούσα μελέτη, διερευνήθηκε η αντιγλοιωματική δράση της κουρκουμίνης και ο μηχανισμός αυτής σε 2 κυτταρικές σειρές ανθρώπινου γλοιοβλαστώματος in vitro, είτε ως μονοθεραπεία, είτε συνδυαστικά με τρέχουσες θεραπευτικές προσεγγίσεις, όπως είναι η ακτινοθεραπεία και η χημειοθεραπεία με τον εγκεκριμένο για γλοιοβλάστωμα αλκυλιωτικό παράγοντα τεμοζολομίδη. Για το σκοπό αυτό, αξιολογήθηκε η επίδραση της κουρκουμίνης στον κυτταρικό πολλαπλασιασμό με τη δοκιμασία κυτταροτοξικότητας μεθυλ-τριαζολυλ-τετραζολίου (ΜΤΤ), τη χρωστική μικροσκοπίου-Trypan Blue Exclusion Assay και τη χρώση με κρυσταλλικό ιώδες (Crystal Violet). Επιπλέον, διερευνήθηκε η δράση της στον κυτταρικό κύκλο με κυτταρομετρία ροής και η επίδραση στην μεταναστευτική ικανότητα των κυττάρων με την τεχνική της προσωμοίωσης τραύματος δι’ αμυχής. Ο έλεγχος της ύπαρξης ή όχι συνέργειας μεταξή κουρκουμίνης και ακτινοθεραπείας ή κουρκουμίνης και τεμοζολομίδης αξιολογήθηκε με την τεχνική Trypan Blue και το λογισμικό CompuSyn (με χρήση του Δείκτη Συνδυασμού CI). Η επίδραση της κουρκουμίνης στην έκφραση της προαποπτωτικής πρωτείνης Bcl2, καθώς και της προ-κασπάσης 3 αξιολογήθηκε με ανοσοαποτύπωση κατά Western blot. Τέλος, πραγματοποιήθηκε δοκιμασία τοξικότητας της κουρκουμίνης σε μοντέλο ιχθύος ζέβρα (zebrafish). Με βάση τα αποτελέσματα της παρούσας διατριβής, η κουρκουμίνη ανέστειλλε σημαντικά τον κυτταρικό πολλαπλασιασμό και στις 2 κυτταρικές σειρές και προκάλεσε δοσο-εξαρτώμενη παύση του κυτταρικού κύκλου στη φάση G2/M. Η κουρκουμίνη επίσης, ανέστειλε τη μετανάστευση των κυττάρων και στις δύο σειρές, μείωσε τα επίπεδα της πρωτείνης Bcl2, καθώς και της προ-κασπάσης 3, υποδεικνύοντας ότι η δράση της σχετίζεται με επαγωγή της απόπτωσης. Επιπλέον, η προκατεργασία των κυττάρων με αυξανόμενες συγκεντρώσεις κουρκουμίνης πριν από την έκθεση σε ακτινοβολία μείωσε τη βιωσιμότητα των κυττάρων σε υψηλότερο βαθμό από τη μονοθεραπεία με κουρκουμίνη ή ακτινοβολία (συνεργική δράση). Η συγχορήγηση κουρκουμίνης και τεμοζολομίδης εμφάνισε επίσης συνεργική δράση στους περισσότερους συνδυασμούς. Τέλος, σε in vivo μοντέλο zebrafish, η μέση θανατηφόρος συγκέντρωση (LC50) της κουρκουμίνης ήταν χαμηλότερη από τις τιμές IC50 για τις 2 κυτταρικές σειρές, επομένως η κουρκουμίνη εμφανίζει μεγαλύτερη εκλεκτικότητα προς τα καρκινικά κύτταρα. Τα αποτελέσματά μας καταδεικνύουν ότι η κουρκουμίνη είναι ένας πολλά υποσχόμενος αντιγλοιωματικός παράγοντας που μπορεί να ευαισθητοποίησει τα κύτταρα του γλοιοβλαστώματος στη δράση της ακτινοθεραπείας και της χημειοθεραπείας. Glioblastoma is one of the deadliest tumors of the central nervous system, with an average patient survival of approximately 15 months after diagnosis. Current treatment options based on surgical resection followed by a combination of chemotherapy and radiotherapy do not significantly increase patient survival. For this reason, the need to find new safe and effective antiglioma agents is considered essential. In this context, many modern research efforts have turned to the study of plant extracts or molecules isolated from plants. Curcumin is a polyphenol isolated from the plant Curcuma longa and has shown significant anticancer properties in various types of neoplasms. In the present study, the antiglioma activity of curcumin and its underlying mechanism were investigated in 2 human glioblastoma cell lines in vitro, either as monotherapy or in combination with current therapeutic approaches, such as radiotherapy and chemotherapy with the approved alkylating agent temozolomide. For this purpose, the effect of curcumin on cell proliferation was evaluated by methyl-triazolyl-tetrazolium (MTT) cytotoxicity assay, Trypan Blue Exclusion Assay and Crystal Violet staining. In addition, its effect on cell cycle progression was investigated by flow cytometry and the effect on the migratory ability of the cells by the scratch wound healing assay. Testing for synergism of curcumin and radiotherapy or curcumin and temozolomide was assessed by the Trypan Blue Exclusion Assay and CompuSyn software (based on the measurement of Combination Index). The effect of curcumin on the expression of the pro-apoptotic protein Bcl2, as well as pro-caspase 3 was evaluated by Western blot immunoprinting. Finally, a toxicity test of curcumin was performed in a zebrafish model. Based on the results of the present thesis, curcumin significantly inhibited cell proliferation in both cell lines and induced dose-dependent cell cycle arrest in the G2/M phase. Curcumin also inhibited cell migration in both lines, reduced the levels of Bcl2 protein, as well as pro-caspase 3, indicating that its antiproliferative action is related to the induction of apoptosis. In addition, pretreatment of cells with increasing concentrations of curcumin before exposure to radiation reduced cell viability to a greater extent than monotherapy with curcumin or radiation (synergistic effect). Co-administration of curcumin and temozolomide also showed a synergistic effect in most tested combinations. Finally, in an in vivo zebrafish model, the mean lethal concentration (LC50) of curcumin was lower compared to its IC50 values against glioblastoma cells, suggesting that curcumin shows more selectivity towards cancer cells. Our results demonstrate that curcumin is a promising antiglioma agent that can sensitize glioblastoma cells to both radiotherapy and chemotherapy. 159 σ.
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- 2023
12. Μελέτη της αντικαρκινικής δράσης της λινεαρόλης σε κυτταρικές σειρές γλοιοβλαστώματος
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Zoi, Vasiliki, Αλεξίου, Γεώργιος, Βούλγαρης, Σπυρίδων, and Γαλάνη, Βασιλική
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Ακτινοθεραπεία ,Linearol ,Φυτικές ουσίες ,Λινεαρόλη ,Radiotherapy ,Γλοιοβλάστωμα ,Natural compounds ,Glioblastoma - Abstract
Το γλοιοβλάστωμα είναι ένας από τους πιο θανατηφόρους όγκους του κενρικού νευρικού συστήματος, με μέση επιβίωση των ασθενών περίπου 15 μήνες μετά τη διάγνωση. Οι τρέχουσες θεραπευτικές επιλογές που βασίζονται στην χειρουργική εκτομή, ακολουθούμενη από συνδυασμό χημειοθεραπείας και ακτινοθεραπείας δεν αυξάνουν σημαντικά την επιβίωση των ασθενών. Για το λόγο αυτό, η ανάγκη εύρεσης νέων ασφαλών και αποτελεσματικών αντικαρκινικών παραγόντων κρίνεται απαραίτητη. Στο πλαίσιο αυτό, οι τρέχουσες ερευνητικές προσπάθειες έχουν στραφεί στην μελέτη φυτικών εκχυλισμάτων ή μορίων που απομονώνονται από φυτά. Η λινεαρόλη είναι ένα διτερπένιο τύπου ent-καουρανίου που απομονώνεται από φυτά του γένους Sideritis και έχει επιδείξει σημαντικές αντιφλεγμονώδεις, αντιοξειδωτικές και αντιμικροβιακές ιδιότητες. Στην παρούσα μελέτη, διερευνήθηκε η πιθανή αντικαρκινική δράση της λινεαρόλης σε κυτταρικές σειρές ανθρωπινου γλοιοβλαστώματος μόνη ή σε συνδυασμό με ακτινοθεραπεία. Για το σκοπό αυτό χρησιμοποιήθηκαν οι σειρές U87 και Τ98 και αξιολογήθηκε η επίδραση της λινεαρόλης στον κυτταρικό πολλαπλασιασμό με τη μέθοδο Trypan Blue Exclusion Assay, η δράση της στον κυτταρικό κύκλο με κυτταρομετρία ροής και η επίδραση στην μεταναστευτική ικανότητα των κυττάρων με την τεχνική της προσωμοίωσης τραύματος δι’ αμυχής. Ο έλεγχος της ύπαρξης ή όχι συνέργειας μεταξή λινεαρόλης και ακτινοθεραπείας αξιολογήθηκε με την τεχνική Trypan Blue και το λογισμικό CompuSyn. Η λινεαρόλη ανέστειλλε σημαντικά τον κυτταρικό πολλαπλασιασμό και προκάλεσε στάση του κυτταρικού κύκλου στη φάση S. Επιπλέον, η προκατεργασία των κυττάρων Τ98 με αυξανόμενες συγκεντρώσεις λινεαρόλης πριν από την έκθεση σε ακτινοβολία 2Gy μείωσε τη βιωσιμότητα των κυττάρων σε υψηλότερο βαθμό από τη μονοθεραπεία με λινεαρόλη ή ακτινοβολία. Η λινεαρόλη επίσης, ανέστειλε τη μετανάστευση κυττάρων και στις δύο δοκιμασμένες κυτταρικές σειρές. Τα αποτελέσματά μας καταδεικνύουν για πρώτη φορά ότι η λινεαρόλη είναι ένας πολλά υποσχόμενος ανιτγλοιωματικός παράγοντας και απαιτούνται περαιτέρω μελέτες για την πλήρη κατανόηση του υποκείμενου μηχανισμού της κυτταροτοξικής της δράσης. Glioblastoma is one of the deadliest tumors of the central nervous system, with an average patient survival of approximately 15 months after diagnosis. Current treatment options based on surgical resection followed by a combination of chemotherapy and radiotherapy do not significantly increase patient survival. For this reason, the need to find new safe and effective anticancer agents is considered essential. In this context, current research efforts have moved towards the study of plant extracts or molecules isolated from plants. Linearol is an ent-kaurane-type diterpene isolated from plants of the genus Sideritis and has shown significant anti-inflammatory, antioxidant and antimicrobial properties. In the present study, the potential antitumor activity of linearol was investigated in human glioblastoma cell lines alone or in combination with radiotherapy. For this purpose, the U87 and T98 lines were used and the effect of linearol on cell proliferation was evaluated by the Trypan Blue Exclusion Assay, its effect on cell cycle by flow cytometry and the effect on the migratory capacity of the cells by the scratch wound healing assay. In order to determine the existence or not of a synergistic relationship between linearol and radiotherapy the Trypan Blue Exclusion Assay was used along with CompuSyn software. Linearol significantly inhibited cell proliferation and induced cell cycle arrest in S phase. Furthermore, pretreatment of T98 cells with increasing concentrations of linearol prior to exposure to 2Gy radiation reduced cell viability to a greater extent than linearol or radiation monotherapy. Linearol also inhibited cell migration in both tested cell lines. Our results demonstrate for the first time that linearol is a promising antiglioma agent, and further studies are needed to fully understand the underlying mechanism of its cytotoxic activity. 79 σ.
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- 2023
13. Assessment of Antioxidant and Antimutagenic Properties of Red and White Wine Extracts In Vitro
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Fotios Tekos, Sotiria Makri, Zoi-Vasiliki Skaperda, Anastasia Patouna, Kallirroi Terizi, Ioannis D. Kyriazis, Yorgos Kotseridis, Eleni Vaskani Mikropoulou, Georgios Papaefstathiou, Maria Halabalaki, and Kouretas Demetrios
- Subjects
wine ,antioxidants ,polyphenols ,free radicals ,oxidative stress ,Microbiology ,QR1-502 - Abstract
Wine is an alcoholic beverage of complex composition obtained through the fermentation of grape must. The consumption of wine has already been associated with a multitude of beneficial effects due to its high polyphenolic content. In this study, four Greek emblematic wines from two red (i.e., Xinomavro and Agiorgitiko) and two white (i.e., Assyrtiko and Malagouzia) varieties were analyzed for the estimation of their antioxidant profiles. To address this question, we assessed their ability to scavenge both synthetic and endogenous free radicals, such as DPPH•, ABTS+•, OH•, O2−, their potential reducing power, and their antimutagenic and antigenotoxic properties. All varieties exhibited potent antioxidant activity, as indicated by the results of methods above, with the red wines appearing more effective than the white ones regarding antioxidant capacity. Our small-scale study is the first to reveal that these wine varieties may have the ability to scavenge the most reactive endogenous radicals. In the future, this finding must be accompanied by larger studies to fill a knowledge gap in the scientific literature concerning a holistic approach of the in vitro antioxidant action of plant polyphenolic compounds. Conclusively, we believe that wines possess high bioactivity that allow them to settle in the industry of food additives and medicinal products.
- Published
- 2021
- Full Text
- View/download PDF
14. Therapeutic Potential of Linearol in Combination with Radiotherapy for the Treatment of Glioblastoma In Vitro
- Author
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Zoi, Vasiliki, primary, Papagrigoriou, Theodora, additional, Tsiftsoglou, Olga S., additional, Alexiou, George A., additional, Giannakopoulou, Maria, additional, Tzima, Eftychia, additional, Tsekeris, Pericles, additional, Zikou, Anastasia, additional, Kyritsis, Athanasios P., additional, Lazari, Diamanto, additional, and Galani, Vasiliki, additional
- Published
- 2023
- Full Text
- View/download PDF
15. Intraoperative Flow Cytometry for the Evaluation of Meningioma Grade
- Author
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Alexiou, George A., primary, Markopoulos, Georgios S., additional, Vartholomatos, Evrysthenis, additional, Goussia, Anna C., additional, Dova, Lefkothea, additional, Dimitriadis, Savvas, additional, Mantziou, Stefania, additional, Zoi, Vasiliki, additional, Nasios, Anastasios, additional, Sioka, Chrissa, additional, Kyritsis, Athanasios P., additional, Voulgaris, Spyridon, additional, and Vartholomatos, George, additional
- Published
- 2023
- Full Text
- View/download PDF
16. Siderol Inhibits Proliferation of Glioblastoma Cells and Acts Synergistically with Temozolomide
- Author
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Giannakopoulou, Maria, primary, Dimitriadis, Kiriakos, additional, Koromili, Maria, additional, Zoi, Vasiliki, additional, Vartholomatos, Evrysthenis, additional, Galani, Vasiliki, additional, Kyritsis, Athanassios P., additional, Alexiou, George A., additional, and Lazari, Diamanto, additional
- Published
- 2022
- Full Text
- View/download PDF
17. COVID-19 presenting with persistent hiccup and myocardial infarction in a peritoneal dialysis patient: A case report
- Author
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Bacharaki, Dimitra, primary, Giannakopoulos, Panagiotis, additional, Markakis, Konstantinos, additional, Papas, Christos, additional, Theodorou, Aikaterini, additional, Zoi, Vasiliki, additional, Tsivgoulis, Georgios, additional, and Lionaki, Sophia, additional
- Published
- 2022
- Full Text
- View/download PDF
18. Calciphylaxis: A Long Road to Cure with a Multidisciplinary and Multimodal Approach
- Author
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Zoi, Vasiliki, primary, Bacharaki, Dimitra, additional, Sardeli, Aggeliki, additional, Karagiannis, Minas, additional, and Lionaki, Sophia, additional
- Published
- 2022
- Full Text
- View/download PDF
19. Clinical presentation and outcomes of chronic dialysis patients with COVID-19: A single center experience from Greece
- Author
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Bacharaki, Dimitra, primary, Karagiannis, Minas, additional, Sardeli, Aggeliki, additional, Giannakopoulos, Panagiotis, additional, Tziolos, Nikolaos Renatos, additional, Zoi, Vasiliki, additional, Piliouras, Nikitas, additional, Arkoudis, Nikolaos-Achilleas, additional, Oikonomopoulos, Nikolaos, additional, Tzannis, Kimon, additional, Kavatha, Dimitra, additional, Antoniadou, Anastasia, additional, Vlahakos, Demetrios, additional, and Lionaki, Sophia, additional
- Published
- 2022
- Full Text
- View/download PDF
20. Radiosensitization and Radioprotection by Curcumin in Glioblastoma and Other Cancers
- Author
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Zoi, Vasiliki, primary, Galani, Vasiliki, additional, Tsekeris, Pericles, additional, Kyritsis, Athanasios P., additional, and Alexiou, George A., additional
- Published
- 2022
- Full Text
- View/download PDF
21. Association of Melatonin Administration in Pregnant Ewes with Growth, Redox Status and Immunity of Their Offspring
- Author
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Bouroutzika, Efterpi, primary, Ciliberti, Maria Giovanna, additional, Caroprese, Mariangela, additional, Theodosiadou, Ekaterini, additional, Papadopoulos, Serafeim, additional, Makri, Sotiria, additional, Skaperda, Zoi-Vasiliki, additional, Kotsadam, Georgios, additional, Michailidis, Marios-Lazaros, additional, Valiakos, George, additional, Chadio, Stella, additional, Kouretas, Dimitris, additional, and Valasi, Irene, additional
- Published
- 2021
- Full Text
- View/download PDF
22. Assessment of Antioxidant and Antimutagenic Properties of Red and White Wine Extracts In Vitro
- Author
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Sotiria Makri, Kouretas Demetrios, Maria Halabalaki, Zoi-Vasiliki Skaperda, Eleni V Mikropoulou, Fotios Tekos, Yorgos Kotseridis, Georgios Papaefstathiou, Ioannis D. Kyriazis, Anastasia Patouna, and Kallirroi Terizi
- Subjects
Antioxidant ,food.ingredient ,DPPH ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,free radicals ,Biochemistry ,Microbiology ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,food ,medicine ,oxidative stress ,Food science ,wine ,Molecular Biology ,polyphenols ,030304 developmental biology ,Wine ,0303 health sciences ,ABTS ,Food additive ,food and beverages ,QR1-502 ,antioxidants ,chemistry ,Polyphenol ,White Wine ,030220 oncology & carcinogenesis ,Fermentation - Abstract
Wine is an alcoholic beverage of complex composition obtained through the fermentation of grape must. The consumption of wine has already been associated with a multitude of beneficial effects due to its high polyphenolic content. In this study, four Greek emblematic wines from two red (i.e., Xinomavro and Agiorgitiko) and two white (i.e., Assyrtiko and Malagouzia) varieties were analyzed for the estimation of their antioxidant profiles. To address this question, we assessed their ability to scavenge both synthetic and endogenous free radicals, such as DPPH•, ABTS+•, OH•, O2−, their potential reducing power, and their antimutagenic and antigenotoxic properties. All varieties exhibited potent antioxidant activity, as indicated by the results of methods above, with the red wines appearing more effective than the white ones regarding antioxidant capacity. Our small-scale study is the first to reveal that these wine varieties may have the ability to scavenge the most reactive endogenous radicals. In the future, this finding must be accompanied by larger studies to fill a knowledge gap in the scientific literature concerning a holistic approach of the in vitro antioxidant action of plant polyphenolic compounds. Conclusively, we believe that wines possess high bioactivity that allow them to settle in the industry of food additives and medicinal products.
- Published
- 2021
23. Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
- Author
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Zoi, Vasiliki, primary, Galani, Vasiliki, additional, Vartholomatos, Evrysthenis, additional, Zacharopoulou, Natalia, additional, Tsoumeleka, Eftichia, additional, Gkizas, Georgios, additional, Bozios, Georgios, additional, Tsekeris, Pericles, additional, Chousidis, Ieremias, additional, Leonardos, Ioannis, additional, Tzakos, Andreas G., additional, Kyritsis, Athanasios P., additional, and Alexiou, George A., additional
- Published
- 2021
- Full Text
- View/download PDF
24. The Role of Curcumin in Cancer Treatment
- Author
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Zoi, Vasiliki, primary, Galani, Vasiliki, additional, Lianos, Georgios D., additional, Voulgaris, Spyridon, additional, Kyritsis, Athanasios P., additional, and Alexiou, George A., additional
- Published
- 2021
- Full Text
- View/download PDF
25. Modelling human variability in toxicokinetic and toxicodynamic processes using Bayesian meta‐analysis, physiologically‐based modelling and in vitro systems
- Author
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Emma Di Consiglio, Emanuela Testai, Zoi-Vasiliki Skaperda, Emma E.J. Kasteel, Nicoletta Santori, Susanna Vichi, Keyvin Darney, Laura Turco, Nynke I. Kramer, Dimitrios Kouretas, Franca M. Buratti, Camille Béchaux, and Leonie S. Lautz
- Subjects
Human variability ,Toxicodynamics ,Bayesian probability ,Computational biology ,Biology - Published
- 2021
- Full Text
- View/download PDF
26. Assessment of Antioxidant and Antimutagenic Properties of Red and White Wine Extracts In Vitro
- Author
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Tekos, Fotios Makri, Sotiria Skaperda, Zoi-Vasiliki Patouna, Anastasia Terizi, Kallirroi Kyriazis, Ioannis D. Kotseridis, Yorgos Mikropoulou, Eleni Vaskani Papaefstathiou, Georgios and Halabalaki, Maria Demetrios, Kouretas
- Subjects
food and beverages - Abstract
Wine is an alcoholic beverage of complex composition obtained through the fermentation of grape must. The consumption of wine has already been associated with a multitude of beneficial effects due to its high polyphenolic content. In this study, four Greek emblematic wines from two red (i.e., Xinomavro and Agiorgitiko) and two white (i.e., Assyrtiko and Malagouzia) varieties were analyzed for the estimation of their antioxidant profiles. To address this question, we assessed their ability to scavenge both synthetic and endogenous free radicals, such as DPPH center dot, ABTS(+center dot), OH center dot, O-2(-), their potential reducing power, and their antimutagenic and antigenotoxic properties. All varieties exhibited potent antioxidant activity, as indicated by the results of methods above, with the red wines appearing more effective than the white ones regarding antioxidant capacity. Our small-scale study is the first to reveal that these wine varieties may have the ability to scavenge the most reactive endogenous radicals. In the future, this finding must be accompanied by larger studies to fill a knowledge gap in the scientific literature concerning a holistic approach of the in vitro antioxidant action of plant polyphenolic compounds. Conclusively, we believe that wines possess high bioactivity that allow them to settle in the industry of food additives and medicinal products.
- Published
- 2021
27. Assessment of Antioxidant and Antimutagenic Properties of Red and White Wine Extracts In Vitro
- Author
-
Tekos, Fotios, primary, Makri, Sotiria, additional, Skaperda, Zoi-Vasiliki, additional, Patouna, Anastasia, additional, Terizi, Kallirroi, additional, Kyriazis, Ioannis D., additional, Kotseridis, Yorgos, additional, Mikropoulou, Eleni Vaskani, additional, Papaefstathiou, Georgios, additional, Halabalaki, Maria, additional, and Demetrios, Kouretas, additional
- Published
- 2021
- Full Text
- View/download PDF
28. Whey protein concentrate improves antioxidant capacity, faecal microbiota and fatty acid profile of growing piglets
- Author
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N Goutzourelas, Konstantinos Gerasopoulos, Sotiria Makri, Ioannis Kafantaris, N. D. Moschonas, Stylianos Kokkas, D. Kantas, Chrysanthi Mitsagga, Konstantinos Petrotos, B. Kotsampasi, Ioannis Giavasis, V. Koukoumis, Zoi-Vasiliki Skaperda, D. Stagos, and Dimitris Kouretas
- Subjects
chemistry.chemical_classification ,Whey protein ,Antioxidant ,biology ,Thiobarbituric acid ,medicine.medical_treatment ,0402 animal and dairy science ,Fatty acid ,04 agricultural and veterinary sciences ,Glutathione ,040401 food science ,040201 dairy & animal science ,Lactic acid ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Catalase ,Genetics ,medicine ,TBARS ,biology.protein ,Animal Science and Zoology ,Food science ,Agronomy and Crop Science - Abstract
A feeding trial involving growing piglets was undertaken to establish whether feed supplemented with whey protein concentrate (WPC), exhibiting antioxidant properties, had any effects on welfare and meat quality. For that purpose, 48 weaned piglets (20-days-old) were assigned to two experimental groups receiving standard or experimental diet for 30 days. Blood and tissue collection were performed at various time-points. The following oxidative stress markers were assessed: reduced glutathione (GSH), catalase activity, total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), protein carbonyls (CARB) and hydrogen peroxide (H2O2) decomposition activity. The effects on bacterial growth and the fatty acid profile of meat were also assessed. Results showed that piglets fed with the WPC-supplemented diet had significantly increased antioxidant mechanisms in almost all tissues tested, as indicated by increases in GSH, H2O2 decomposition activity and TAC compared with the control group. Piglets fed with the experimental diet exhibited decreased oxidative stress-induced damage to lipids and proteins, as shown by decreases in TBARS and CARB in the WPC group compared with the control group. In addition, the experimental diet enhanced growth of facultative probiotic bacteria and lactic acid bacteria and inhibited growth of pathogen populations. In addition, WPC inclusion in piglets' diet increased n-3 fatty acids significantly and decreased n-6/n-3 ratio significantly compared with the control group. The current study showed that WPC inclusion in the diet had a significant effect on welfare and meat quality of growing piglets.
- Published
- 2019
- Full Text
- View/download PDF
29. Modelling human variability in toxicokinetic and toxicodynamic processes using Bayesian meta‐analysis, physiologically‐based modelling and in vitro systems
- Author
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Testai, Emanuela, primary, Bechaux, Camille, additional, Buratti, Franca M., additional, Darney, Keyvin, additional, Di Consiglio, Emma, additional, Kasteel, Emma E.J., additional, Kramer, Nynke I., additional, Lautz, Leonie S., additional, Santori, Nicoletta, additional, Skaperda, Zoi‐Vasiliki, additional, Kouretas, Dimitrios, additional, Turco, Laura, additional, and Vichi, Susanna, additional
- Published
- 2021
- Full Text
- View/download PDF
30. Association of Melatonin Administration in Pregnant Ewes with Growth, Redox Status and Immunity of Their Offspring
- Author
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E. Theodosiadou, Georgios Kotsadam, S. Chadio, George Valiakos, I. Valasi, Sotiria Makri, Zoi-Vasiliki Skaperda, Marios-Lazaros Michailidis, S. Papadopoulos, Mariangela Caroprese, Efterpi Bouroutzika, Maria Giovanna Ciliberti, and Dimitris Kouretas
- Subjects
Antioxidant ,IgG ,Thiobarbituric acid ,Offspring ,Veterinary medicine ,animal diseases ,medicine.medical_treatment ,melatonin ,lambs ,Article ,Melatonin ,chemistry.chemical_compound ,Animal science ,SF600-1100 ,medicine ,TBARS ,General Veterinary ,biology ,antioxidant biomarkers ,Glutathione ,cytokines ,QL1-991 ,prenatal stress ,chemistry ,biology.protein ,Colostrum ,Animal Science and Zoology ,Antibody ,Zoology ,medicine.drug - Abstract
In this study, the effects of melatonin treatment on growth, redox status and immunity in prenatally stressed newborn lambs were evaluated. Thirty-seven newborn lambs were allocated into two groups (melatonin-MEL and control-CON), based on whether their mothers were treated with melatonin implants or not, respectively. All pregnant ewes were exposed to heat stress. The body weight of lambs was recorded at birth (L0), and then on days 15 (L15) and 40 (L40). Redox biomarkers [total antioxidant capacity (TAC), glutathione (GSH), thiobarbituric acid reactive substances (TBARS)] were assayed in blood samples collected from lambs on days L0, L1, L2, L5, L10 and L40. Chemical analysis and antioxidant capacity were evaluated in colostrum and milk samples collected at the same time points with blood samples. Cytokines (IL-1β, IL-6, IL-10, IFN-γ) and immunoglobulin (IgG) were assayed in blood and colostrum samples collected from ewes on days L0 and L1, and in lambs’ blood on days L0, L1 and L2. The results revealed that body weight gain of newborn lambs did not differ between the two groups (p >, 0.05). Better redox status was found in MEL lambs until L2, as well as higher antioxidant capacity in the colostrum of MEL ewes compared to CON ones on day L0 (p <, 0.05). In MEL ewes’ colostrum, higher protein content was measured on day L0 and higher fat content on L1 compared to CON group (p <, 0.05). The highest level of IL-6 was found in MEL ewes on L1, with a concomitant increase of IL-10 level in MEL lambs in comparison to CON lambs on L2. Moreover, CON colostrum resulted in a higher level of IL-10 within time, coupled with an increased level of IgG found in lambs’ plasma on L2 (p = 0.04). This study indicated that melatonin could be administered as antioxidant and immune-modulatory regime in prenatally stressed offspring in order to cope with the crucial first days of their life. This effect of melatonin was also amplified by crosstalk between IL-6, IL-10 and IgG production, resulting in an improved quality of produced milk.
- Published
- 2021
- Full Text
- View/download PDF
31. In vitro and in vivo redox-related effects of nanomaterials: A comprehensive approach
- Author
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Periklis Vardakas, Demetrios Kouretas, A.-F. Trompeta, Aristidis Tsatsakis, Costas A. Charitidis, Zoi-Vasiliki Skaperda, and Fotios Tekos
- Subjects
Chemistry ,In vivo ,Biophysics ,General Medicine ,Toxicology ,Redox ,In vitro ,Nanomaterials - Published
- 2021
- Full Text
- View/download PDF
32. Adverse Health Effects of Environmental Noise.
- Author
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Zoi, Vasiliki and Papanastasiou, Theofilos
- Subjects
BIBLIOMETRICS ,MEDLINE ,NOISE ,ONLINE information services ,PUBLIC health ,SYSTEMATIC reviews ,ENVIRONMENTAL exposure ,RESIDENTIAL patterns - Published
- 2014
33. A Novel Fluorescent Gemcitabine Prodrug That Follows a Nucleoside Transporter-Independent Internalization and Bears Enhanced Therapeutic Efficacy With Respect to Gemcitabine.
- Author
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Vrettos EΙ, Kyrkou SG, Zoi V, Giannakopoulou M, Chatziathanasiadou MV, Kanaki Z, Agalou A, Bistas VP, Kougioumtzi A, Karampelas T, Diamantis DA, Murphy C, Beis D, Klinakis A, Tamvakopoulos C, Kyritsis AP, Alexiou GA, and Tzakos AG
- Subjects
- Humans, Animals, Mice, Cell Line, Tumor, Endocytosis drug effects, Fluoresceins chemistry, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Nucleoside Transport Proteins metabolism, Drug Liberation, Gemcitabine, Deoxycytidine analogs & derivatives, Deoxycytidine chemistry, Deoxycytidine pharmacology, Prodrugs chemistry, Prodrugs pharmacology, Zebrafish, Fluorescent Dyes chemistry
- Abstract
The multiplexity of cancer has rendered it the second leading cause of mortality worldwide and theragnostic prodrugs have gained popularity in recent years as a means of treatment. Theragnostic prodrugs enable the simultaneous diagnosis and therapy of tumors via high-precision real-time drug release monitoring. Herein, we report the development of the small theragnostic prodrug GF, based on the nucleoside anticancer agent gemcitabine and the fluorescent dye 5(6)-carboxyfluorescein. We have successfully demonstrated its efficient internalization in tumor cells, showing localization throughout both the early and late endocytic pathways. Its mechanism of cell internalization was evaluated, confirming its independence from nucleoside transporters. Its cellular localization via confocal microscopy revealed a clathrin-mediated endocytosis mechanism, distinguishing it from analogous compounds studied previously. Furthermore, GF exhibited stability across various pH values and in human blood plasma. Subsequently, its in vitro cytotoxicity was assessed in three human cancer cell lines (A549, U87 and T98). Additionally, its pharmacokinetic profile in mice was investigated and the consequent drug release was monitored. Finally, its in vivo visualization was accomplished in zebrafish xenotransplantation models and its in vivo efficacy was evaluated in A549 xenografts. The results unveiled an intriguing efficacy profile, positioning GF as a compelling candidate warranting further investigation., (© 2024 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
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