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Your search keyword '"Zlateski, Vladimir"' showing total 7 results
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7 results on '"Zlateski, Vladimir"'

1. Engineering inorganic nanomaterials for the capturing, storage and release of biomolecules

Author

Zlateski, Vladimir, Stark, Wendelin J., and Pérez-Ramírez, Javier

Subjects
ENZYME + ENZYMOLOGIE (BIOCHEMIE), SILIKATE UND SILICIUMDIOXID (ANORGANISCHE CHEMIE), NANOBIOTECHNOLOGIE, DESOXYRIBONUKLEINSÄUREN (NUKLEINSÄUREN), ADSORPTION + ABSORPTION + DESORPTION, NANOBIOTECHNOLOGY, ENZYMES + ENZYMOLOGY (BIOCHEMISTRY), DEOXYRIBONUCLEIC ACIDS (NUCLEIC ACIDS), SILICATES AND SILICON DIOXIDE (INORGANIC CHEMISTRY), Engineering & allied operations, ddc:620
Published
2016
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7. Protein Reduction and Dialysis-Free Work-Up through Phosphines Immobilized on a Magnetic Support: TCEP-Functionalized Carbon-Coated Cobalt Nanoparticles.

Author

Zwyssig A, Schneider EM, Zeltner M, Rebmann B, Zlateski V, Grass RN, and Stark WJ

Abstract

Tris(2-carboxyethyl)phosphine (TCEP) is an often-used reducing agent in biochemistry owing to its selectivity towards disulfide bonds. As TCEP causes undesired consecutive side reactions in various analytical methods (e.g., gel electrophoresis, protein labeling), it is usually removed by means of dialysis or gel filtration. Here, an alternative method of separation is presented, namely the immobilization of TCEP on magnetic nanoparticles. This magnetic reagent provides a simple and rapid approach to remove the reducing agent after successful reduction. A reduction capacity of 70 μmol per gram of particles was achieved by using surface-initiated atom transfer polymerization., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2017
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