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Abstract Purpose Cervical cancer (CC), a significant global health threat, necessitates comprehensive understanding for improved therapeutic interventions. Many research indicates that dysregulation of the Hippo-YAP1 pathway leads to uncontrolled proliferation and invasion of tumor cells, promoting the progression of various cancers. This article aims to elucidate the role of RNF6 in CC and its regulation of the Hippo-YAP1 signaling pathway. Methods The public tumor dataset analyses, immunohistochemistry, and western blotting were used to explore the expression of RNF6 in CC. Gain- and loss-of-function assays were conducted to elucidate the role of RNF6 in the proliferation and invasion of CC cells. Transcriptome sequencing was used to explore RNF6’s role in cervical cancer, with validation of its regulation of the Hippo-YAP1 pathway through western blotting and RT-qPCR. Co-transfection of YAP overexpression plasmids into RNF6-silenced CC cells were preformed to confirm YAP1’s pivotal role in RNF6-mediated CC progression. Animal experiments were preformed to further validate RNF6 interference’s inhibitory effect on CC proliferation in vivo. Results Clinical samples and bioinformatics analysis revealed high expression of RNF6 in CC, and closely associated with advanced FIGO (International Federation of Gynecology and Obstetrics) stage, larger tumor size, and poor prognosis. Cellular functional experiments demonstrate that RNF6 promotes the proliferation, invasion, and migration of CC cells, while knockdown of RNF6 yields the opposite effect. Transcriptome sequencing further reveals that RNF6 may promote CC progression through the Hippo-YAP signaling pathway. Western blotting and RT-qPCR further unveil that RNF6 enhances the upregulation of YAP1 protein levels, thereby activating downstream oncogenes CTGF and CYR61 transcription. Additionally, exogenous overexpression of YAP1 reverses the inhibitory effect of RNF6 silencing on CC proliferation and invasion. Furthermore, RNF6 interference significantly attenuates tumor growth in vivo experiments. Conclusion Our research reveals that RNF6 is highly expressed in CC, driving malignant progression by upregulating YAP1 protein expression and enhancing the transcription of downstream target genes CTGF and CYR61, offering potential therapeutic targets for CC treatment.

Abstract To develop new types of dynamic molecular devices with atomic-scale control over electronic function, new types of molecular switches are needed with time-dependent switching probabilities. We report such a molecular switch based on proton-coupled electron transfer (PCET) reaction with giant hysteric negative differential resistance (NDR) with peak-to-valley ratios of 120 ± 6.6 and memory on/off ratios of (2.4 ± 0.6) × 103. The switching dynamics probabilities are modulated by bias voltage sweep rate and can also be controlled by pH and relative humidity, confirmed by kinetic isotope effect measurements. The demonstrated dynamical and environment-specific modulation of giant NDR and memory effects provide new opportunities for bioelectronics and artificial neural networks.

Abstract Photodetection has attracted significant attention for information transmission. While the implementation relies primarily on the photonic detectors, they are predominantly constrained by the intrinsic bandgap of active materials. On the other hand, photothermoelectric (PTE) detectors have garnered substantial research interest for their promising capabilities in broadband detection, owing to the self-driven photovoltages induced by the temperature differences. To get higher performances, it is crucial to localize light and heat energies for efficient conversion. However, there is limited research on the energy conversion in PTE detectors at micro/nano scale. In this study, we have achieved a two-order-of-magnitude enhancement in photovoltage responsivity in the self-rolled tubular tellurium (Te) photodetector with PTE effect. Under illumination, the tubular device demonstrates a maximum photovoltage responsivity of 252.13 V W−1 and a large detectivity of 1.48 × 1011 Jones. We disclose the mechanism of the PTE conversion in the tubular structure with the assistance of theoretical simulation. In addition, the device exhibits excellent performances in wide-angle and polarization-dependent detection. This work presents an approach to remarkably improve the performance of photodetector by concentrating light and corresponding heat generated, and the proposed self-rolled devices thus hold remarkable promises for next-generation on-chip photodetection.

The harmonization of rural human -land relations, centered on the interaction between rural livelihoods and land use, is crucial for sustainable rural development. This study explores the relationship between rural households’ livelihood capital status and land use effects (LUEs) in Qufu City, Shandong Province, China. The analysis is based on data collected through a triangulation method, combining quantitative (questionnaire) and qualitative (interview) approaches. Using a coupling degree (CD) model and a coupling coordination degree (CCD) model within a human -land system framework, this study evaluates the coupling and coordination between rural households’ livelihoods and land use as subsystems. It also examines the impact of LUE and CCD on rural households’ livelihood strategies through Skinner’s reinforcement theory. Results indicate variable CD and CCD across households with different livelihood strategies. In particular, the CD for the four household types ranged from 0.9 to 1, denoting a high-level coupling stage, with off-farm employment-oriented households registering the highest degree of 0.996. In CCD, professional households achieved a high degree of 0.761, whereas the others displayed a medium coupling coordination stage between 0.5 and 0.7. Professional households and balanced households showed superior LUE and CCD, with more than 50% of these households planning to maintain their original strategies. By contrast, households with off-farm employment-oriented or conventional strategies exhibited low LUE and CCD, with fewer than 50% of these households planning to retain their initial strategies. Professional agriculture-based livelihood strategies help improve the livelihood levels of farmers and promote the rational use of cultivated land resources, thereby making them particularly attractive to households engaged in agricultural production. This research contributes insights valuable for promoting the sustainable development of rural households’ livelihoods and land use.

Proton exchange membrane fuel cells (PEMFCs) have been widely acknowledged as a significant advancement in achieving sustainable energy conversion. However, the activation of newly established Pt-ionomer interfaces in the catalyst layer of PEMFCs can be a time-consuming and costly process to ensure proper coupling and performance. In order to gain valuable insights into this crucial activation process, we have conducted a comprehensive analysis and comparison of the commonly employed on-line (such as current or voltage control activation, short-circuiting activation, and air interruption activation) and off-line (including boiling or steaming, acid-treatment, and ultrasonic-treatment) activation methods. Our findings shed light on the underlying mechanisms that contribute to enhanced performance within the catalyst layer, such as the reduction of Pt oxides and hydroxides, improved proton transport, and the reduction of “dead” regions. Moreover, this review emphasizes the significant challenges and future opportunities that lie in further enhancing the performance within the catalyst layer through the activation process.

Inner ear hair cell (HC) damage is irreversible in mammals, but it has been shown that supporting cells (SCs) have the potential to differentiate into HCs. Serpine2, a serine protease inhibitor, encodes protease nexin 1, and this has been suggested to be a factor that promotes HC regeneration. In this study, we overexpressed Serpine2 in inner ear SCs cultured in two- and three-dimensional systems using the adeno-associated virus-inner ear (AAV-ie) vector, which promoted organoid expansion and HC differentiation. Overexpression of Serpine2 in the mouse cochlea through the round window membrane (RWM) injection promoted SC proliferation and HC regeneration, and the regenerated HCs were found to be derived from Lgr5+ SCs. Regenerated HCs have electrophysiological properties that are similar to those of native HCs. Notably, Serpine2 overexpression promoted HC survival and restored hearing of neomycin-damaged mice. In conclusion, our findings indicate that Serpine2 overexpression promotes HC regeneration and suggests that the utilization of inner ear progenitor cells in combination with AAVs might be a promising therapeutic target for hearing restoration.

Hepatitis B virus (HBV) infection poses a challenge to global public health. Persistent liver infection with HBV is associated with an increased risk of developing severe liver disease. The complex interaction between the virus and the host is the reason for the persistent presence of HBV and the risk of tumor development. Chronic liver inflammation, integration of viral genome with host genome, expression of HBx protein, and viral genotype are all key participants in the pathogenesis of hepatocellular carcinoma (HCC). Epigenetic regulation in HBV-associated HCC involves complex interactions of molecular mechanisms that control gene expression and function without altering the underlying DNA sequence. These epigenetic modifications can significantly affect the onset and progression of HCC. This review summarizes recent research on the epigenetic regulation of HBV persistent infection and HBV-HCC development, including DNA methylation, histone modification, RNA modification, non-coding RNA, etc. Enhanced knowledge of these mechanisms will offer fresh perspectives and potential targets for intervention tactics in HBV-HCC.

ObjectiveTo explore any correlation between serum urate (SU) level or insulin resistance (IR) and metabolic dysfunction associated steatotic liver disease (MASLD) in patients with metabolic syndrome (MS).MethodsData from all MASLD patients, diagnosed by liver biopsy, were enrolled and divided into MASLD alone group and MASLD with MS group. They were subdivided into hyperuricemia group and normal SU group to find correlation between SU/IR and MASLD in patients with MS and independent risk factors for MASLD.ResultsData from 539 MASLD patients were analyzed. Body mass index (BMI) (p = 0.000), waist circumference (WC) (p = 0.004), and low-density lipoprotein (LDL) (p = 0.000) were dramatically higher in MASLD with MS group than those with MASLD alone; MASLD with MS patients had significantly more family history of diabetes (p = 0.000) and hypertension (p = 0.000) than patients with MASLD alone. Height (p = 0.000), weight (p = 0.000), BMI (p = 0.000) and WC (p = 0.001), and LDL (p = 0.007) were dramatically higher in hyperuricemia patients than those with normal SU. SU was inversely associated with age (p = 0.000) and high-density lipoprotein (HDL) (p = 0.003), and positively correlated with weight (p = 0.000), BMI (p = 0.000) and WC (p = 0.000), TG (p = 0.000), and LDL (p = 0.000). Logistic Regression analysis showed that age (p = 0.031), TG (p = 0.002), LDL (p = 0.010), HbA1c (p = 0.026), and family history of hypertension (p = 0.000) may be independent risk factors for MASLD in patient with MS.ConclusionInsulin resistance (IR) in MASLD patients with MS, but not higher SU levels, has closer correlation with the occurrence of MASLD in patients with family history of hypertension and diabetes having higher BMI, LDL, HbA1c.

Protein elicitors can induce plant systemic resistance to pathogens. The recognition of a potential elicitor activates intracellular signaling events, leading to plant resistance against pathogens. In this study, a novel protein elicitor was isolated from the culture filtrate of Verticillium nonalfalfae and named PeVn1, which can induce cell death in several plant species. The PeVn1 gene was then cloned and expressed in Escherichia coli. The recombinant protein PeVn1 triggers cell death in Nicotiana benthamiana in NbBAK1 and NbSOBIR1 dependent manner. Through bioassay analysis showed that the recombinant PeVn1 induced early defense induction events, such as reactive oxygen species burst, callose deposition and the activation of defense hormone signaling pathways and defense enzyme activities. Moreover, PeVn1 significantly enhanced resistance of Nicotiana benthamiana to Sclerotinia sclerotiorum, Botrytis cinerea and N. benthamiana mosaic virus and tomato to Pseudomonas syringae pv. Tomato DC3000. In conclusion, our study reveals that PeVn1 protein as a microbe-associated molecular pattern can induce plant immune responses, which provides a theoretical basis for the development of novel protein-induced disease resistance agents.

Niobium carbide (NbC) is often used as a reinforcing phase to improve the mechanical properties of composite coatings, but its low thermal conductivity makes the flow of molten pool poor, and the cladding layer is prone to defects such as non-fusion and slag inclusion. Therefore, in this paper, Ni60–NbC composite coatings with different NbC content were prepared by laser cladding technology, and the 10 vol% graphene-20 vol% NbC-70 vol% Ni60 composite coatings were prepared under the optimal NbC component. The effects of the addition of NbC and graphene on the microstructure and microhardness of the coating were systematically discussed. The results show that the microhardness of 20 vol% NbC composite coating is increased from 680.1 HV to 882.9 HV compared with Ni60 coating without NbC.The composite coating with both NbC and graphene has a microhardness of up to 1048HV, which is 18% higher than the composite coating with only 20 vol% NbC. The mechanism is that the addition of graphene promotes the formation of chromium carbide (Cr23C6, Cr7C3) and increases the fluidity of the molten pool, which causes small niobium carbide particles to dissolve and recondense to form large particles. Large niobium carbide particles and chromium carbide jointly inhibit the excessive growth of other grains. This study provides a new idea for laser cladding of nickel-based composite coatings.

Abstract Releasing pre-strained two-dimensional nanomembranes to assemble on-chip three-dimensional devices is crucial for upcoming advanced electronic and optoelectronic applications. However, the release process is affected by many unclear factors, hindering the transition from laboratory to industrial applications. Here, we propose a quasistatic multilevel finite element modeling to assemble three-dimensional structures from two-dimensional nanomembranes and offer verification results by various bilayer nanomembranes. Take Si/Cr nanomembrane as an example, we confirm that the three-dimensional structural formation is governed by both the minimum energy state and the geometric constraints imposed by the edges of the sacrificial layer. Large-scale, high-yield fabrication of three-dimensional structures is achieved, and two distinct three-dimensional structures are assembled from the same precursor. Six types of three-dimensional Si/Cr photodetectors are then prepared to resolve the incident angle of light with a deep neural network model, opening up possibilities for the design and manufacturing methods of More-than-Moore-era devices.

This is an observational retrospective study analyzed the performance of the Chinese women's national field hockey team during the 2020 Tokyo Olympics and 2021 National Games to assess the impact of opposition quality on performance. Game statistics were collected using notational analysis software for 76 Olympic and 40 National Games matches. Non-parametric Mann-Whitney U tests were used to compare tournaments. No significant differences existed for 35 out of 38 metrics, except Offense in 25-Yd Area (P = 0.013), Handball Style (P = 0.000) and Entry into Arc - Right Lane (P = 0.017). When exclusively considering Chinese national team's observations, superior National Games performance did emerge for Shot (P = 0.046), Goal from Short Corner (P = 0.044), Into the Arc (P = 0.046), Entry into Arc - Q3 (P = 0.009), Dribble into the Arc (P = 0.046), Handball Style into Arc (P = 0.041), Forehand Shot (P = 0.033), and Small Skill Shot (P = 0.014). The study underscores the influence of opposition quality on team performance, with a need for targeted improvements in arc penetration efficacy, conversion rates of shots to goals, and adaptation of tactical approaches against stronger defenses. The research points towards the need for strategic high-performance programs, improved domestic league quality, and a structured youth development system to elevate the overall standard of Chinese field hockey to achieve global competitiveness.

Abstract Mammalian cochlear hair cells (HCs) are essential for hearing, and damage to HCs results in severe hearing impairment. Damaged HCs can be regenerated by neighboring supporting cells (SCs), thus the functional regeneration of HCs is the main goal for the restoration of auditory function in vivo. Here, cochlear SC trans‐differentiation into outer and inner HC by the induced expression of the key transcription factors Atoh1 and its co‐regulators Gfi1, Pou4f3, and Six1 (GPAS), which are necessary for SCs that are destined for HC development and maturation via the AAV‐ie targeting the inner ear stem cells are successfully achieved. Single‐cell nuclear sequencing and lineaging tracing results showed that the majority of new Atoh1‐derived HCs are in a state of initiating differentiation, while GP (Gfi1, Pou4f3) and GPS (Gfi1, Pou4f3, and Six1) enhanced the Atoh1‐induced new HCs into inner and outer HCs. Moreover, the patch‐clamp analysis indicated that newborn inner HCs induced by GPAS forced expression have similar electrophysiological characteristics to those of native inner HCs. Also, GPAS can induce HC regeneration in the HC‐damaged mice model. In summary, the study demonstrates that AAV‐mediated co‐regulation of multiple genes, such as GPAS, is an effective means to achieve functional HC regeneration in the mouse cochlea.

BackgroundThere is a growing interest in the use of complementary therapies for the prevention of disease and the maintenance of health. Furthermore, complementary therapies that incorporate exercise are becoming increasingly prevalent among the older adult, and thus may represent a crucial strategy for the primary and secondary prevention of cardiovascular disease (CVD). Exercise therapy, as a means to prevent and treat cardiovascular diseases, has been gradually applied in clinical practice. It has the advantages of reducing mortality, improving clinical symptoms, restoring physical function and improving quality of life. In recent years, traditional Chinese sports such as Ba Duan Jin and Qigong have developed rapidly. Therefore, a comprehensive systematic review is required to examine interventions involving Ba Duan Jin exercise in healthy adults or those at increased risk of CVD in order to determine the effectiveness of Ba Duan Jin exercise for the primary prevention of CVD.ObjectiveTo investigate the effect of Ba Duan Jin exercise intervention for the primary prevention of cardiovascular diseases.MethodsEight databases were systematically searched from inception to July, 2024 for randomized controlled trials (RCTs) to evaluated the impact of Ba Duan Jin exercise intervention on cardiovascular diseases. The search terms were “Cardiovascular diseases” “Ba Duan Jin” and “Randomized controlled.” The Cochrane risk assessment tool was used to evaluate the study quality, and the meta-analysis was performed using Rev. Man 5.4 software.ResultsSeventeen completed trials were conducted with 1,755 participants who were randomly assigned and met the inclusion criteria. All 17 studies were conducted in China. The meta-analysis indicates that Ba Duan Jin exercise therapy can provide long-term benefits (20–30 years) by reducing all-cause mortality (RR = 0.55, 95% CI: 0.44–0.68, p

Visceral white adipose tissues (WAT) regulate systemic lipid metabolism and inflammation. Dysfunctional WAT drive chronic inflammation and facilitate atherosclerosis. Adipose tissue-associated macrophages (ATM) are the predominant immune cells in WAT, but their heterogeneity and phenotypes are poorly defined during atherogenesis. The scavenger receptor CD36 mediates ATM crosstalk with other adipose tissue cells, driving chronic inflammation. Here, we combined the single-cell RNA sequencing technique with cell metabolic and functional assays on major WAT ATM subpopulations using a diet-induced atherosclerosis mouse model (Apoe-null). We also examined the role of CD36 using Apoe/Cd36 double-null mice. Based on transcriptomics data and differential gene expression analysis, we identified a previously undefined group of ATM displaying low viability and high lipid metabolism and labeled them as “unhealthy macrophages”. Their phenotypes suggest a subpopulation of ATM under lipid stress. We also identified lipid-associated macrophages (LAM), which were previously described in obesity. Interestingly, LAM increased 8.4-fold in Apoe/Cd36 double-null mice on an atherogenic diet, but not in Apoe-null mice. The increase in LAM was accompanied by more ATM lipid uptake, reduced adipocyte hypertrophy, and less inflammation. In conclusion, CD36 mediates a delicate balance between lipid metabolism and inflammation in visceral adipose tissues. Under atherogenic conditions, CD36 deficiency reduces inflammation and increases lipid metabolism in WAT by promoting LAM accumulation.

The transformation of energy structure has brought about new changes in the power system, and the environmentally friendly gas switchgear with the goal of low carbon and environmental protection has been widely spread and applied, but due to its short application time and the accumulation of related research, it continues to carry out life prediction to improve the related operation and maintenance system. The article proposes the environmental protection gas switchgear data processing technology based on data enhancement technology, proposes the environmental protection gas switchgear life prediction technology based on approximate dynamic planning, and verifies the algorithm through case analysis, which proves the effectiveness and accuracy of the proposed method.

Background Lenvatinib plus PD-1 inhibitors and interventional (LPI) therapy have demonstrated promising treatment effects in unresectable hepatocellular carcinoma (HCC). However, biomarkers for predicting the response to LPI therapy remain to be further explored. We aimed to develop a radiomics model to noninvasively predict the efficacy of LPI therapy.Methods Clinical data of patients with HCC receiving LPI therapy were collected in our institution. The clinical model was built with clinical information. Nine machine learning classifiers were tested and the multilayer perceptron classifier with optimal performance was used as the radiomics model. The clinical-radiomics model was constructed by integrating clinical and radiomics scores through logistic regression analysis.Results 151 patients were enrolled in this study (2:1 randomization, 101 and 50 in the training and validation cohorts), of which three achieved complete response, 69 showed partial response, 46 showed stable disease, and 33 showed progressive disease. The objective response rate, disease control rate, and conversion resection rates were 47.7, 78.1 and 23.2%. 14 features were selected from the initially extracted 1223 for radiomics model construction. The area under the curves of the radiomics model (0.900 for training and 0.893 for validation) were comparable to that of the clinical-radiomics model (0.912 for training and 0.892 for validation), and both were superior to the clinical model (0.669 for training and 0.585 for validation). Meanwhile, the radiomics model can categorize participants into high-risk and low-risk groups for progression-free survival (PFS) and overall survival (OS) in the training (HR 1.913, 95% CI 1.121 to 3.265, p=0.016 for PFS; HR 4.252, 95% CI 2.051 to 8.816, p=0.001 for OS) and validation sets (HR 2.347, 95% CI 1.095 to 5.031, p=0.012 for PFS; HR 2.592, 95% CI 1.050 to 6.394, p=0.019 for OS).Conclusion The promising machine learning radiomics model was developed and validated to predict the efficacy of LPI therapy for patients with HCC and perform risk stratification, with comparable performance to clinical-radiomics model.

Abstract Circular RNAs (circRNAs) have been implicated in tumorigenesis and progression of various cancers. However, the underlying mechanisms of circRNAs in hepatocellular carcinoma (HCC) have not been fully elucidated. Herein, a new oncogenic circRNA, hsa_circ_0070039 (circNUP54), was identified to be significantly upregulated in HCC through circRNA sequencing. As verified in 68 HCC samples, circNUP54 overexpression was correlated with aggressive cancerous behaviors and poor outcomes. Moreover, the function experiments showed that knockdown of circNUP54 inhibited the malignant progression of HCC in vitro and in vivo, whereas overexpression of circNUP54 had the opposite role. Mechanistic investigations carried out by RNA pull-down, RNA immunoprecipitation, and immunofluorescence revealed that circNUP54 interacted with the RNA-binding protein Hu-antigen R (HuR) and promoted its cytoplasmic export. The cytoplasmic accumulation of HuR stabilized the downstream BIRC3 mRNA through its binding to the 3′ UTR region. Consequently, the encoded protein of BIRC3, cellular inhibitor of apoptosis 2 (cIAP2), proceeded to activate the NF-κB signal pathway and ultimately contributed to HCC progression. In addition, depletion of BIRC3 rescued the pro-tumorigenic effect of circNUP54 on HCC cells. Overall, this study demonstrated that circNUP54 facilitates HCC progression via regulating the HuR/BIRC3/NF-κB axis, which may serve as a promising therapeutic target for HCC treatment.

Abstract Corticotropin-releasing hormone-binding protein (CRHBP) is involved in many physiological processes. However, it is still unclear what role CRHBP has in tumor immunity and prognosis prediction. Using databases such as the Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Tumor Protein Database, Timer Database, and Gene Expression Profiling Interactive Analysis (GEPIA), we evaluated the potential role of CRHBP in diverse cancers. Further research looked into the relationships between CRHBP and tumor survival prognosis, immune infiltration, immune checkpoint (ICP) indicators, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), DNA methylation, tumor microenvironment (TME), and drug responsiveness. The anticancer effect of CRHBP in liver hepatocellular carcinoma (LIHC) was shown by Western blotting, EdU staining, JC-1 staining, transwell test, and wound healing assays. CRHBP expression is significantly low in the majority of tumor types and is associated with survival prognosis, ICP markers, TMB, and microsatellite instability (MSI). The expression of CRHBP was found to be substantially related to the quantity of six immune cell types, as well as the interstitial and immunological scores, showing that CRHBP has a substantial impact in the TME. We also noticed a link between the IC50 of a number of anticancer medicines and the degree of CRHBP expression. CRHBP-related signaling pathways were discovered using functional enrichment. Cox regression analysis showed that CRHBP expression was an independent prognostic factor for LIHC. CRHBP has a tumor suppressor function in LIHC, according to cell and molecular biology trials. CRHBP has a significant impact on tumor immunity, treatment, and prognosis, and has the potential as a cancer treatment target and prognostic indicator.

Abstract Metal organic framework (MOF) films have attracted abundant attention due to their unique characters compared with MOF particles. But the high‐temperature reaction and solvent corrosion limit the preparation of MOF films on fragile substrates, hindering further applications. Fabricating macro‐sized continuous free‐standing MOF films and transferring them onto fragile substrates are a promising alternative but still challenging. Here, a universal strategy to prepare transferrable macro‐sized continuous free‐standing MOF films with the assistance of oxide nanomembranes prepared by atomic layer deposition and studied the growth mechanism is developed. The oxide nanomembranes serve not only as reactant, but also as interfacial layer to maintain the integrality of the free‐standing structure as the stacked MOF particles are supported by the oxide nanomembrane. The centimeter‐scale free‐standing MOF films can be transferred onto fragile substrates, and all in one device for glucose sensing is assembled. Due to the strong adsorption toward glucose molecules, the obtained devices exhibit outstanding performance in terms of high sensitivity, low limit of detection, and long durability. This work opens a new window toward the preparation of MOF films and MOF film‐based biosensor chip for advantageous applications in post‐Moore law period.

Abstract Complete remission of colorectal cancer (CRC) is still unachievable in the majority of patients by common fractionated radiotherapy, leaving risks of tumor metastasis and recurrence. Herein, clinical CRC samples demonstrated a difference in the phosphorylation of translation initiation factor eIF2α (p‐eIF2α) and the activating transcription factor 4 (ATF4), whose increased expression by initial X‐ray irradiation led to the resistance to subsequent radiotherapy. The underlying mechanism is studied in radio‐resistant CT26 cells, revealing that the incomplete mitochondrial outer membrane permeabilization (iMOMP) triggered by X‐ray irradiation is key for the elevated expression of p‐eIF2α and ATF4, and therefore radio‐resistance. This finding guided to discover that metformin and 2‐DG are synergistic in reversing radio resistance by inhibiting p‐eIF2α and ATF4. Liposomes loaded with metformin and 2‐DG (M/D‐Lipo) are thus prepared for enhancing fractionated radiotherapy of CRC, which achieved satisfactory therapeutic efficacy in both local and metastatic CRC tumors by reversing radio‐resistance and preventing T lymphocyte exhaustion.

Abstract Background The factors affecting the cumulative live birth rate (CLBR) of PCOS (Polycystic ovary syndrom) patients who received in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) needs more research for a better outcome. Methods Here we carried out a retrospective analysis of 1380 PCOS patients who received IVF/ICSI-ET for the first time from January 2014 to December 2016. We divided them into cumulative live birth group (group A) and non-cumulative live birth group (group B) according to whether there were live births. Results The conservative cumulative live birth rate was 63.48%. There were 876 cumulative live births (group A) and 504 non-cumulative live births (group B) according to whether the patients had live births or not. Competition analysis showed that duration of infertility, primary/secondary type of infertility, stimulation protocols, starting dose of gonadotrophins and oocyte retrieved numbers were significantly correlated with CLBR. The Cox proportional risk regression model of PCOS patients showed that stimulation protocols had a significant impact on CLBR. Patients in the GnRH (Gonadotropin-releasing hormone)-antagonist protocol group and the mild stimulation protocol had lower CLBR than those in the prolonged GnRH-agonist protocol, which was statistically significant. PCOS patients with the starting dose of gonadotrophins greater than 112.5u had lower CLBR than those with less than 100u, which was statistically significant. Women with 11–15 oocytes and 16–20 oocytes had higher CLBR than women with 1–9 oocytes, which was statistically significant. Conclusions When we used Prolonged GnRH-agonist protocol, or the first starting dose of gonadotrophins was 100u-112.5u, or the number of oocytes obtained was 11–15 and 16–20, the CLBR of PCOS patients increased significantly after the 1st oocyte collection.

The joining of zirconium alloys is an important issue in the field of nuclear energy since zirconium alloys are often used as nuclear fuel cladding tube. The article reviews the research progress on the diffusion bonding, brazing and resistance welding of zirconium alloys. The main focus of this work is on the process parameters, interfacial microstructure and mechanical properties of joint. For diffusion bonding, the process parameters including temperature and interlayer have significant influence on the properties of joint. The range of temperature and the selection of interlayer was reviewed and the influence of them on interfacial microstructure and properties of joints was also discussed. For brazing, the influence of brazing filler, temperature and holding time on the properties of joint was summarized. For resistance welding, the main process parameters include welding current and contact resistance. Numerical simulation method was often used to describe the influence of process parameters on microstructure during welding end plug with cladding tube. Some suggestions for the further research direction on the welding and joining of zirconium alloys were also proposed.

Sensory hair cells are responsible for detecting and transmitting sound in the inner ear, and damage to HCs leads to hearing loss. HCs do not regenerate spontaneously in adult mammals, which makes the hearing loss permanent. However, hair cells and supporting cells have the same precursors in the inner ear, and in newborn mice, the adjacent SCs can be activated by gene manipulation to differentiate into newly regenerated hair cells. Here, we demonstrate the role of the Ras association domain family member 2 (Rassf2) in supporting cell to hair cell trans-differentiation in the inner ear. Using the AAV vector (AAV-ie) to upregulate Rassf2 expression promoted supporting cell division and hair cell production in cultured cochlear organoids. Also, AAV-Rassf2 enhanced the regenerative ability of Lgr5+ SCs in the postnatal cochlea without impairing hearing, and this might due to the modulation of the Wnt, Hedgehog and Notch signaling pathways. Furthermore, AAV-Rassf2 enhances cochlear supporting cell division and hair cell production in the neomycin injury model. In summary, our results suggest that Rassf2 is a key component in HC regenerative repair, and gene modulation mediated by adeno-associated virus may be a promising gene therapy for hearing repair.

ABSTRACTGenetic editing is a powerful tool for functional characterization of genes in various organisms. With its simplicity and specificity, the CRISPR-Cas9 technology has become a popular editing tool, which introduces site-specific DNA double-strand breaks (DSBs), and then leverages the endogenous repair pathway for DSB repair via homology-directed repair (HDR) or the more error-prone non-homologous end joining (NHEJ) pathways. However, in the Plasmodium parasites, the lack of a typical NHEJ pathway selects for DSB repair through the HDR pathway when a homologous DNA template is available. The AT-rich nature of the Plasmodium genome exacerbates this drawback by making it difficult to clone longer homologous repair DNA templates. To circumvent these challenges, we adopted the hybrid catalytically inactive Cas9 (dCas9)-microbial single-stranded annealing proteins (SSAP) editor to the Plasmodium genome. In Plasmodium yoelii, we demonstrated the use of the dCas9-SSAP, as the cleavage-free gene editor, by targeted gene deletion and gene tagging, even using shorter homologous DNA templates. This dCas9-SSAP method with a shorter DNA template, which did not require DSBs, independent of HDR and NHEJ, would be a great addition to the existing genetic toolbox and could be deployed for the functional characterization of genes in Plasmodium, contributing to improving the ability of the malaria research community in characterizing more than half of genes with unknown functions.IMPORTANCEMalaria caused by Plasmodium parasites infection remains a serious threat to human health, with an estimated 249 million malaria cases and 608,000 deaths worldwide in 2022, according to the latest report from the World Health Organization (WHO). Here, we demonstrated the use of dCas9-single-stranded annealing protein, as the cleavage-free gene editor in Plasmodium yoelii, by targeted deletion and gene tagging, even using shorter homologous DNA templates. This method with a shorter DNA template, which did not require DSBs, independent of HDR and NHEJ, showing the potential significance in greatly improving our ability to elucidate gene functions, would contribute to assisting the malaria research community in deciphering more than half of genes with unknown functions to identify new drug and vaccine targets.

Abstract Mutations in OTOFERLIN (OTOF) lead to the autosomal recessive deafness 9 (DFNB9). The efficacy of adeno‐associated virus (AAV)‐mediated OTOF gene replacement therapy is extensively validated in Otof‐deficient mice. However, the clinical safety and efficacy of AAV‐OTOF is not reported. Here, AAV‐OTOF is generated using good manufacturing practice and validated its efficacy and safety in mouse and non‐human primates in order to determine the optimal injection dose, volume, and administration route for clinical trials. Subsequently, AAV‐OTOF is delivered into one cochlea of a 5‐year‐old deaf patient and into the bilateral cochleae of an 8‐year‐old deaf patient with OTOF mutations. Obvious hearing improvement is detected by the auditory brainstem response (ABR) and the pure‐tone audiometry (PTA) in these two patients. Hearing in the injected ear of the 5‐year‐old patient can be restored to the normal range at 1 month after AAV‐OTOF injection, while the 8‐year‐old patient can hear the conversational sounds. Most importantly, the 5‐year‐old patient can hear and recognize speech only through the AAV‐OTOF‐injected ear. This study is the first to demonstrate the safety and efficacy of AAV‐OTOF in patients, expands and optimizes current OTOF‐related gene therapy and provides valuable information for further application of gene therapies for deafness.

Chinese philosophy is a philosophy of ecology. The basic problem of Chinese philosophy is the relationship between heaven and man, that is, the relationship between nature and man. This is evident in the main schools of Chinese philosophy, such as Confucianism and Taoism. A correct understanding and evaluation of Chinese ecological philosophy has important practical significance for us to reflect on the ecological imbalance and environmental deterioration caused by the industrial civilization, created under the guidance of the mechanical concept of nature and anthropocentrism, and to re-examine the way of human existence, development, thinking and values. This paper aims to explore the elements of eco-philosophy in Chinese philosophy and analyze its influence on the concept, value and practice of ecological civilization.

Abstract OTOF mutations are the principal causes of auditory neuropathy. There are reports on Otof‐related gene therapy in mice, but there is no preclinical research on the drug evaluations. Here, Anc80L65 and the mouse hair cell‐specific Myo15 promoter (mMyo15) are used to selectively and effectively deliver human OTOF to hair cells in mice and nonhuman primates to evaluate the efficacy and safety of OTOF gene therapy drugs. A new dual‐AAV‐OTOF‐hybrid strategy to transfer full‐length OTOF is generated, which can stably restore hearing in adult OTOFp.Q939*/Q939* mice with profound deafness, with the longest duration being at least 150 days, and the best therapeutic effect without difference in hearing from wild‐type mice. An AAV microinjection method into the cochlea of cynomolgus monkeys without hearing impairment is further established and found the OTOF can be safely and effectively driven by the mMyo15 promoter in hair cells. In addition, the therapeutic dose of AAV drugs has no impact on normal hearing and does not cause significant systemic toxicity both in mouse and nonhuman primates. In summary, this study develops a potential gene therapy strategy for DFNB9 patients in the clinic and provides complete, standardized, and systematic research data for clinical research and application.

Wetlands, as a crucial component of urban green spaces, provide important leisure services for residents. Construction of wetlands has increased with the rapid urban expansion and population growth in China over recent decades, especially in semi-arid regions with scarce water resources. However, the residents’ satisfaction with wetland leisure demand remains unclear. This study evaluated the residents’ satisfaction with wetland leisure demand by a framework of physical calculation, taking Yinchuan City, the capital city of Ningxia Hui Autonomous Region, in the semi-arid region of Western China as a case study area. Spatial variations in residents’ satisfaction and their causes were revealed by a supply–demand relationship between the population capacity of wetlands and the total population of communities under a framework of physical calculation. The results indicated that 4.22% of the study area, which covered 7.38% of the total population, was fully satisfied with wetland leisure demand. Residents’ satisfaction in the urban area as a whole is low at 0.49, with a concentric distribution pattern increasing as the distance from the central urban area increases. The high population density and scanty wetlands mainly induced a relative-low residents’ satisfaction in the central urban area, accounting for 12.02% of the area and 32.70% of the population. Meanwhile, a relative-high residents’ satisfaction in the outer ring of the central urban area accounting for 59.10% of the area and 20.63% of the population was primarily due to the adequate capacity of wetlands. Medium residents’ satisfaction was mainly attributed to the road network density, which partially transferred local wetland leisure demand to adjacent areas. Wetland constructions and internal structural optimization to improve the capacity for leisure in densely populated central urban areas could provide a feasible path to alleviate unbalanced conditions. These results could deepen the understanding of supply–demand dynamics in the wetland leisure service and provide valuable information for optimizing wetland allocation in urban construction in semi-arid regions.

The Lower Paleozoic of the Awati Sag and its periphery is a region with relatively low levels of exploration and stands as a frontier for ultra-deep hydrocarbon exploration. Based on outcrop and core samples, this study integrated organic geochemical analysis, total organic carbon (TOC) logging interpretation, and one-dimensional and two-dimensional hydrocarbon accumulation simulations, to clarify the primary source rock of the Lower Paleozoic and its characteristics, as well as its hydrocarbon accumulation mode. The findings indicate the following: (1) The Lower Paleozoic features two sets of industrial source rocks. The Yuertusi Formation, with its considerable thickness (approximately 200 m), widespread distribution, and elevated TOC (averaging approximately 5% from experimental data and logging interpretation), stands out as the Lower Paleozoic’s most pivotal source rock. (2) The Yuertusi and Saergan Formations are in a high-to-over-mature stage, with the Yuertusi initiating oil generation in the early Silurian and transitioning to gas by the late Permian. The Saergan began producing oil in the Carboniferous, followed by gas in the late Permian. (3) The potential ultra-deep gas reservoirs in the Awati Sag are mainly distributed in the structural traps closer to the deep faults in five potential target formations. Deep natural gas typically exhibits mixed-source signatures, with the mixing notably pronounced along the Shajingzi Fault Belt due to influential basin-controlling faults.

Membrane separation technology plays a vital role in natural gas processing due to its cost-effectiveness, excellent efficiency and low energy consumption. In this study, we demonstrate the superior permeability and selection capability of the graphene kirigami (GK) membrane for natural gas purification via molecular dynamics insights. The results indicate that the separation rate of the GK membrane can reach up to 4.1 × 106 GPU (gas permeance unit) with nearly 100% selectivity of the permeating gases mixture (H2S, CO2 and N2) over the non-permeating gas (CH4). Furthermore, the Monte Carlo simulation reveals that the unique geometry of the GK structure introduces a funnel-shaped adsorption channel to guide specific molecules’ separation and enhance the efficiency of the membrane. Compared with the nano-porous graphene (NPG) membrane, the gas separation rate of the GK membrane can be increased by more than four times. Moreover, the GK membrane does not require the modification of functional groups in natural gas purification, which provides great convenience for future applications in practical projects. The considerable potential of the GK membrane underpinned by the findings of this study will attract broad interest in the field of separation and purification.

In this paper, CrCoNi medium-entropy alloy coatings were prepared by laser cladding process, and ultrasonic impact treatment (UIT) with different impact amplitudes (0 μm, 10 μm, 15 μm, 20 μm) was performed on the coating surface to systematically study the microstructure and properties of the coatings. The results showed that the surface deformation of the coatings became more and more severe with the increase of ultrasonic impact amplitude. After the UIT treatment, the impact layer with obvious plastic deformation appeared on the top of the coating, and the depth of the impact layer gradually increased with the increase of the amplitude, and the influence range of the impact layer could be roughly judged according to the increase of the small-angle grain boundary on the top, which was about 200–400 μm at the amplitude of 20 μm. The grain size was 64.067 μm at an amplitude of 20 μm, which was about 34% of the original size. The microhardness of the coating was found to increase with the impact amplitude, and when the impact amplitude reached 20 μm, the microhardness of the coating reached 374.1 HV0.2, which was 122% higher than that of the unimpacted coating. Also, the wear resistance of the Cr–Co–Ni coating increased with the increase of impact amplitude, and the wear weight loss of the coating increased by 85.71% compared to the untreated specimen at an impact amplitude of 20 μm.

Since the 20th century, the world is facing two major problems: the serious deterioration of the ecological environment and the accelerated decline of living quality of people residing in the urban environment. Urban development policies, ecological patterns, production and consumption modes have an important impact on global environmental changes that threaten the future living of mankind. The focus of urban space construction has also turned to the fields of ecological security, energy development, resource management and urban ecological virtuous circulation. Investigating urban spatial structure features from the perspective of urban metabolic system is important for ecological security and resource management. Shangluo urban space in Qinling area is used as the research object and an analysis system of urban spatial structure based on ecological network analysis is proposed in this study. First, an ecological network model of urban space was constructed from the research perspective of ecological network analysis and the system metabolic level was evaluated using metabolic scale, intensity, and efficiency. Second, the system structure of urban metabolic network and its functional relations were analyzed. On this basis, interactive relationships among different components in the metabolic system were explored via symbiosis index and symbiotic, competitive, predatory, and control relations. Meanwhile, the ecological network analysis method is used to construct an analysis system for urban spatial structure in the Qinling mountain area. Finally, the analysis system of urban spatial structure in the Qinling mountain area is applied to examine the urban spatial structure of Shangluo, Qinling Mountains, China. Results showed that resource utilization efficiency of Shangluo City presents a rising trend and the city is located in a strong structural center in the regional space. Predatory and control relations played a dominant role among ecological relations in the urban metabolic structure, and the urban space presented a clear uniaxially extended spatial structure. Sensitivity of internal environment was extremely evident, and the natural spatial structure was an important basis for urban space development in Shangluo. The urban spatial structure analysis system in this research is constructed with the three aspects of “metabolic level”, “ecological relationship” and “compartment weight” of the spatial structure information, which solves the problem that the conventional spatial structure analysis system is difficult to quantify the ecological network information, and it provides a new idea to explain the internal component interaction mechanism and spatial structure composition of the urban system. The results of this study can provide not only useful reference for urban spatial structure analysis of underdeveloped ecological capital areas in China but also a basis for sustainable development planning of urban spaces.

Objective: Adolescents experience the critical period for physical and psychological growth. Few studies focus on the influence of sleep hygiene on the psychological health of adolescence aging from high school to freshmen year. Also, the influence from the COVID-19 pandemic has a public health significance. Methods: A total of 698 students from high schools and colleges were included in the manuscript, and a cross-sectional procedure was conducted, objective to make an epidemiological comparison of the social phobia/depression prevalence, and discuss the effects of potential determinants. Results: Psychological problems including social phobia and depression were prevalent especially among the high school students, with the female gender showing higher possibilities. Current results also indicated that the association between sleep status and the occurrence of social phobia is most obvious among high school students, while relatively higher MMR risks was found both for high school students showing symptoms of social phobia and college freshmen with depressive symptoms. Moreover, the interaction between social phobia and depression was obvious for both populations. Conclusions: Psychological problems including social phobia symptoms and depression are more prevalent among the high school females when compared with their male and freshemen peers. Sgnificant influencial factors for the risk of psychological problems among 2 populations are different, but media multitasking status should be paid attention to for both.

Tendinopathy is a medical condition that includes a spectrum of inflammatory and degenerative tendon changes caused by traumatic or overuse injuries. The pathological mechanism of tendinopathy has not been well defined, and no ideal treatment is currently available. Platelet-rich plasma (PRP) is an autologous whole blood derivative containing a variety of cytokines and other protein components. Various basic studies have found that PRP has the therapeutic potential to promote cell proliferation and differentiation, regulate angiogenesis, increase extracellular matrix synthesis, and modulate inflammation in degenerative tendons. Therefore, PRP has been widely used as a promising therapeutic agent for tendinopathy. However, controversies exist over the optimal treatment regimen and efficacy of PRP for tendinopathy. This review focuses on the specific molecular and cellular mechanisms by which PRP manipulates tendon healing to better understand how PRP affects tendinopathy and explore the reason for the differences in clinical trial outcomes. This article has also pointed out the future direction of basic research and clinical application of PRP in the treatment of tendinopathy, which will play a guiding role in the design of PRP treatment protocols for tendinopathy.

The biogeography-based optimization (BBO) algorithm is known for its simplicity and low computational overhead, but it often struggles with falling into local optima and slow convergence speed. Against this background, this work presents a multi-strategy enhanced BBO variant, named MSBBO. Firstly, the example chasing strategy is proposed to eliminate the destruction of the inferior solutions to superior solutions. Secondly, the heuristic crossover strategy is designed to enhance the search ability of the population. Finally, the prey search–attack strategy is used to balance the exploration and exploitation. To verify the performance of MSBBO, we compare it with standard BBO, seven BBO variants (PRBBO, BBOSB, HGBBO, FABBO, BLEHO, MPBBO and BBOIMAM) and seven meta-heuristic algorithms (GWO, WOA, SSA, ChOA, MPA, GJO and BWO) on multiple dimensions of 24 benchmark functions. It concludes that MSBBO significantly outperforms all competitors both on convergence accuracy, speed and stability, and MSBBO basically converges to the same results on 10,000 dimensions as on 1000 dimensions. Further, MSBBO is applied to six real-world engineering design problems. The experimental results show that our work is still more competitive than other latest optimization techniques (COA, EDO, OMA, SHO and SCSO) on constrained optimization problems.

Objectives: Epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells (RTECs) is a pathogenic factor for renal interstitial fibrosis (RIF). Interleukin-33 (IL-33) is related to the occurrence and development of RIF, but the underlying mechanism remains unclear. Here, we investigated whether IL-33 mediates the EMT of RTECs by activating the NF-κB/Twist1 signalling pathway. Methods: In vivo, the RIF animal model induced by unilateral ureteral obstruction (UUO) was established. The effects of exogenous IL-33 and anti-IL-33 antibodies were evaluated. In vitro, the EMT of RTECs was induced by IL-33. The inhibition of the nuclear factor kappa-B (NF-κB) by the pyrrolidine dithiocarbamate (PDTC) and the knockdown of the suppression of tumorigenicity 2 (ST2) by small interference RNA were used to observe whether IL-33 mediates the EMT of RTECs through the NF-κB/Twist1 signaling pathway. Results: In vivo, exogenous IL-33 significantly aggravated UUO-induced pathological damage and collagen deposition, down-regulated E-cadherin expression, and up-regulated α-smooth muscle actin and Vimentin expressions. Moreover, exogenous IL-33 increased the levels of phospho-IκB-α (p-IκB-α) and phospho-NF-κB p65 (p-NF-κB p65), NF-κB p65 nuclear translocation, and Twist1 nuclear expression. However, these effects were reversed by the anti-IL-33 antibody. In vitro , the increases in the levels of p-IκB-α, p-NF-κB p65, NF-κB p65 nuclear translocation, and Twist1 nuclear expression induced by IL-33 were inhibited by the knockdown of PDTC or ST2. IL-33-mediated EMT of RTECs was also significantly reversed. However, NF-κB inhibitor PDTC had no significant effect on ST2 expression. Conclusions: The IL-33/ST2 axis may up-regulate the expression of Twist1 through the NF-κB signalling pathway, thereby inducing the EMT of RTECs and leading to RIF.

Abstract As a molecular marker of the female reproductive system, Paired Box 8 is widely used in pathological diagnosis of gynecological tumors, but it is not clear whether its expression level is related to the development of uterine corpus endometrial carcinoma and molecular subtype classifications. Here, we show that PAX8 is up-regulated in TP53 mutation category of UCEC, which is result from the low methylation level of PAX8 in UCEC. We have identified that genes connected to ribosome, lysosome, ribosome biogenesis and cell cycle as PAX8 targets and demonstrate that modulation of the PAX8-DDX5 interaction influences c-MYC related cell cycle and cell growth. Our work defines DDX5 as a critical PAX8 co-factor, places the PAX8-DDX5 interaction in biological context, and highlights PAX8 as a key point for development of novel anti-MYC therapies in TP53-mutation UCEC.

The optimization of water chemistry in pressurized water reactor (PWR) primary circuit is one of the most effective ways to achieve both safety and economy for operating PWR nuclear power plants (NPPs). Special attention has been paid to fundamental research and engineering application of Zn-injection water chemistry (ZWC) in PWR primary circuit in recent years in China due to the rapid development of nuclear power. The present paper mainly reports the status of PWR NPPs in China and some fundamental understandings of ZWC on material corrosion. Effects of temperature (T), pH at T value, Zn concentration, Zn-injection sequence and lasting time on electrochemical corrosion behavior, oxide film characteristic and stress corrosion cracking (SCC) susceptibility of nuclear-grade austenitic alloys were investigated. A modified point defect model was used to discuss the effects of ZWC on the oxide films in high-temperature pressurized water. Some water chemistry parameters in PWR primary coolant system are proposed for mitigating corrosion and SCC of nuclear-grade austenitic alloys.

Abstract Circular RNAs have been reported to play essential roles in the tumorigenesis and progression of various cancers. However, the biological processes and mechanisms involved in hepatocellular carcinoma (HCC) remain unclear. Initial RNA-sequencing data and qRT-PCR results in our cohort showed that hsa_circ_0072309 (also called circLIFR) was markedly downregulated in HCC tissues. Kaplan–Meier analysis indicated that higher levels of circLIFR in HCC patients correlated with favorable overall survival and recurrence-free survival rates. Both in vitro and in vivo experiments indicated that circLIFR inhibited the proliferation and invasion abilities of HCC cells. We therefore conducted related experiments to explore the mechanism of circLIFR in HCC. Fluorescence in situ hybridization results revealed that circLIFR was mainly located in the cytoplasm, and RNA immunoprecipitation assays indicated that circLIFR was significantly enriched by Ago2 protein. These results suggested that circLIFR may function as a sponge of miRNAs to regulate HCC progression. We further conducted bioinformatics prediction as well as dual-luciferase reporter assays, and the results of which showed that circLIFR could sponge miR-624-5p to stabilize glycogen synthase kinase 3β (GSK-3β) expression. Loss and gain of function experiments demonstrated that regulation of the expression of miR-624-5p or GSK-3β markedly affected HCC progression induced by circLIFR. Importantly, we also proved that circLIFR could facilitate the degradation of β-catenin and prevent its translocation to the nucleus in HCC cells. Overall, our study demonstrated that circLIFR acts as a tumor suppressor in HCC by regulating miR-624-5p and inactivating the GSK-3β/β-catenin signaling pathway.

Abstract Background Our previous two-dimensional electrophoresis experiment showed that the expression of LASP1 in patients with endometriosis was significantly higher than that of control endometrium. However, the molecular mechanism by which LASP1 is regulated in endometriosis/adenomyosis is unknown. Methods Herein, qPCR was performed to analyze the expression levels of LASP1 and miR-218-5p between endometriosis (Ems) cells and control cells. Fluorescence in situ hybridization was carried out to measure the expression level of miR-218-5p in ectopic endometrium versus normal endometrium. After miR-218-5p mimic or inhibitor were transfected, the transwell experiment was carried out to see the effect of miR-218-5p on the migration of endometrial stromal cells (ESCs). EdU was used to measure cell proliferation rate. Dual-luciferase reporter assay was used to verify the binding of hsa-miR-218-5p to the 3’UTR of LASP1. Western blot and immunofluorescence analysis were carried out to identify the protein expression pattern of LASP1 and EMT markers in endometrial tissue. Results The miR-218-5p is mainly secreted from blood vessels and expressed in the muscle layer around the endometrium, which inhibits the expression level of LASP1 by binding the 3’UTR region of LASP1 in normal ESCs. Overexpression of miR-218-5p impedes the epithelial-to-mesenchymal transition (EMT) and prevents the migration of ESCs and the expression of Vimentin in Ems. Conclusions Our findings revealed that miR-218-5p in endometrial microenvironment prevents the migration of ectopic endometrial stromal cells by inhibiting LASP1.

This paper presents an anti-swing control method to prevent situations where inspection robots detach and fall off transmission lines during obstacle crossing due to excessive swing angles caused by the rotation of the robot around the transmission line. Firstly, an obstacle-crossing model for the inspection robot was constructed and the causes of robot swinging phenomena were analyzed, in addition to their impact on obstacle crossing stability. By combining this with the obstacle-crossing model, a moment balance equation was established for the inspection robot. This equation can be used to solve mapping relationships between body offset and the tilt angle of transmission line gripping arms. We propose an anti-swing control strategy by adjusting the angle of the transmission line gripping arm’s pitching joint to make the body offset approach zero, and by utilizing the advantages of fuzzy logic in the fuzzy PID algorithm compared with the traditional PID algorithm, it can adaptively avoid the occurrence of robot swinging phenomena. The experimental results of obstacle-crossing experiments under no wind and wind turbulence conditions indicated that the proposed anti-swing control method in this study can effectively keep the body offset to within 3 mm. Compared with the methods of not using anti-swing control and using traditional PID anti-swing control, in the absence of wind effects, the peak values of body offset were reduced by 96.53% and 18.85%, respectively. Under the influence of wind turbulence, the peak values of body offset were reduced by 97.02% and 27.12%, respectively. The effectiveness of the anti-swing control method proposed in this paper has thus been verified.

When a remote sensing camera work in push-broom mode, the obtained image usually contains significant stripe noise and random noise due to differences in detector response and environmental factors. Traditional approaches typically treat them as two independent problems and process the image sequentially, which not only increases the risk of information loss and structural damage, but also faces the situation of noise mutual influence. To overcome the drawbacks of traditional methods, this paper leverages the double low-rank characteristics in the underlying prior of degraded images and presents a novel approach for addressing both destriping and denoising tasks simultaneously. We utilize the commonality that both can be treated as inverse problems and place them in the same optimization framework, while designing an alternating direction method of multipliers (ADMM) strategy for solving them, achieving the synchronous removal of both stripe noise and random noise. Compared with traditional approaches, synchronous denoising technology can more accurately evaluate the distribution characteristics of noise, better utilize the original information of the image, and achieve better destriping and denoising results. To assess the efficacy of the proposed algorithm, extensive simulations and experiments were conducted in this paper. The results show that compared with state-of-the-art algorithms, the proposed method can more effectively suppress random noise, achieve better synchronous denoising results, and it exhibits a stronger robustness.

Corrosion release behavior of Alloy 690 in high-temperature water was investigated under the conditions of injected Zn concentrations of 0 ppb, 10 ppb and 50 ppb. A protective oxide film composed of Zn(FexCr1-x)2O4 and Cr2O3 was formed with Zn injection, resulting in a better corrosion resistance. In comparison with the Zn-free condition, the corrosion release rate under the Zn-injection conditions was smaller. The corrosion release inhibiting factors were 1.7 and 1.9 under the conditions of 10 ppb and 50 ppb Zn-injection respectively. A foreseen application of the corrosion and corrosion release rates has been proposed and discussed.

Surface subsidence induced by underground mining is a typical serious geohazard. Numerical approaches such as the discrete element method (DEM) and finite difference method (FDM) have been widely used to model and analyze mining-induced surface subsidence. However, the DEM is typically computationally expensive, and is not capable of analyzing large-scale problems, while the mesh distortion may occur in the FDM modeling of largely deformed surface subsidence. To address the above problems, this paper presents a geometrically and locally adaptive remeshing method for the FDM modeling of largely deformed surface subsidence induced by underground mining. The essential ideas behind the proposed method are as follows: (i) Geometrical features of elements (i.e. the mesh quality), rather than the calculation errors, are employed as the indicator for determining whether to conduct the remeshing; and (ii) Distorted meshes with multiple attributes, rather than those with only a single attribute, are locally regenerated. In the proposed method, the distorted meshes are first adaptively determined based on the mesh quality, and then removed from the original mesh model. The tetrahedral mesh in the distorted area is first regenerated, and then the physical field variables of old mesh are transferred to the new mesh. The numerical calculation process recovers when finishing the regeneration and transformation. To verify the effectiveness of the proposed method, the surface deformation of the Yanqianshan iron mine, Liaoning Province, China, is numerically investigated by utilizing the proposed method, and compared with the numerical results of the DEM modeling. Moreover, the proposed method is applied to predicting the surface subsidence in Anjialing No. 1 Underground Mine, Shanxi Province, China.

Biogeography-based optimization (BBO) cannot effectively solve high-dimensional global optimization problems due to its single migration mechanism and random mutation operator. To get better performance, a dual BBO based on sine cosine algorithm (SCA) and dynamic hybrid mutation is proposed in this work, which named SCBBO. Firstly, the Latin hypercube sampling method is innovatively used to improve the initial population ergodicity. Secondly, a nonlinear transformation parameter and a inertia weight adjustment factor are designed into the position update formula of SCA to make SCBBO suitable for high dimensional environments. Then, a dynamic hybrid mutation operator is designed by combining Laplacian and Gaussian mutation, which helps the algorithm to escape from local optima and balance the exploration and exploitation. Finally, the dual learning strategy is integrated, so the convergence accuracy is further improved by generating dual individuals. Meanwhile, A sequence convergence model is established to prove the algorithm can converge to the global optimal solution with probability 1. Compared with other state-of-the-art evolutionary algorithms, SCBBO effectively improves the optimization accuracy and convergence speed for high-dimensional optimization problems. To further show the superiority of SCBBO, its performance is compared on 1000, 2000, 5000 and 10000 dimensions, respectively. The comparsions show that SCBBO’s optimization results on these dimensions are basically the same. Applying SCBBO to engineering design problems, and the simulation results demonstrate that the proposed method is also effective on constrained optimization problems.

Abstract Recently it is discovered that molecular junctions can be pushed into the Marcus Inverted region of charge transport, but it is unclear which factors are important. This paper shows that the mechanism of charge transport across molecular wires can be switched between the normal and Marcus Inverted regions by fine‐tuning the molecule–electrode coupling strength and the tunneling distance across oligophenylene ethynylene (OPE) wire terminated with ferrocene (Fc) abbreviated as S‐OPEnFc (n = 1–3). Coherent tunneling dominates the mechanism of charge transport in junctions with short molecules (n = 1), but for n = 2 or 3 redox reactions become important. By weakening the molecule—electrode interaction by interrupted conjugation, S‐CH2‐OPEnFc, intramolecular orbital gating can occur pushing the junctions completely into the Marcus Inverted region. These results indicated that weak molecule—electrode coupling is important to push junctions into the Marcus Inverted Region.

Coastal wetlands, the major component of coastal ecotones with indispensable ecosystem services, are threatened by anthropogenic disturbance, resulting in continuous loss of ecosystem functions. Coastal wetland restoration can be implemented to deter the ecosystem losses; however, it is unclear whether it could provide appropriate habitat for the birds using on coastal ecotones. Here, we utilize a newly restored wetland as an example to investigate the impacts of coastal restoration on bird diversity, and test the hypothesis - if a reasonable habitat pattern is created, more birds will be attracted, thereby helping decision-makers develop efficient and sustainable coastal restoration strategies. We used Fragstats for landscape pattern analysis, and derived the variability in different habitat patterns by independent sample T-test and Mann-Whitney U test. The results suggested that the restored wetland exhibited a positive effect on attracting birds, with a total of 70 species, 35 families, and 15 orders of birds being recorded over a three-year period after restoration. Passeriformes are the main species, and accounted for 52.8% of bird species. Additionally, waterbird species, i.e., Ciconiiformes and Anseriformes, accounted for 24.67% of the total number of species. The number of bird species in the wetlands increased annually, especially during the overwintering and the breeding period. Furthermore, the results of this study indicate that water-centered mosaic-type habitat consisting of a relatively low degree of separation and high patch connectivity was beneficial to attracting different types of birds. The number of bird species, density, bird biodiversity index, evenness index, and dominance index for mosaic-type habitats were all higher than those for even habitat pattern with independent patches and sharp boundaries. In particular, the mosaic-type habitat attracted migratory waterbirds such as Anas zonorhyncha, Aix galericulata, Mareca penelope, Hydrophasianus chirurgus, Emberiza pallasi, Xenus cinereus, and Spatula querquedula, which expanded the range of birds attracted by coastal restoration projects. This study illustrated that coastal wetland restoration combined with a creation of water-centered mosaic-type habitat attracted more birds and could provide a reference for the restoration of degraded ecosystems in coastal zones.

ObjectiveTest anxiety is one of the common psychological and behavioral problems of college students, which can result in poor academic performance and even academic failure. Aromatherapy has been proposed as a promising method to reduce test anxiety in college students, but its precise efficacy has not been fully confirmed. This meta-analysis evaluated the effects of aromatherapy on the symptoms of test anxiety in college students to serve as a reference for future research and provide more scientific and exact evidence.MethodsPubMed, The Cochrane Library, Web of Science, Embase, CINAHL, Science Direct, Chinese National Knowledge Infrastructure (CNKI), Chinese Science and Technology Journal Full-Text Database (VIP), and Wanfang Data were electronically searched from inception to June 2022 to identify randomized controlled trials (RCTs) on aromatherapy for treating students’ test anxiety. The Cochrane Risk of Bias Assessment Tool for RCTs was used by two reviewers to critically and independently assess the methodological quality of the included studies. Review Manager 5.4 was used for the meta-analysis. Stata 17.0 was used for sensitivity analysis and Egger’s test.ResultsSeven RCTs included 425 patients, with a moderate risk of bias in the included studies. The meta-analysis found that aromatherapy effectively reduced test anxiety in college students (SMD = −0.67, p < 0.01), with high heterogeneity of results (I2 = 72%). The different types of essential oil devices used in the study are the source of inter-study heterogeneity. Subgroup analysis suggested that most effective methods were aromatherapy with compound essential oils (SMD = −0.91, p < 0.01), and using cloths or pads as the essential oil devices (SMD = −1.03, p < 0.01). There was no difference between aromatherapy and placebo control groups (SMD = −0.65, p = 0.25).ConclusionAromatherapy with inhalation can help relieve test anxiety in college students, but more and higher-quality studies are needed. This study provides a reference for future research designs in terms of the selection of essential oil types and devices and the design of research groups.Systematic review registration[https://www.crd.york.ac.uk/prospero/], identifier [CRD42022345767].