1. Liposome fusogenic enzyme-free circuit enables high-fidelity determination of single exosomal RNA
- Author
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Zixin Sun, Xiaohui Chen, Ruyan Niu, Hengyi Chen, Ying Zhu, Chong Zhang, Liu Wang, Huaming Mou, Hong Zhang, and Yang Luo
- Subjects
Exosome ,Fusion ,Liposome ,Single-molecule detection ,Tumor ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Accurate determination of single exosomal inclusions in situ presents a significant challenge due to their extremely low abundance as well sub-100 nm vesicle dimensions. Here, we created a Liposome Fusogenic Enzyme-free circuit (LIFE) approach for the high-fidelity identification of exosome-encapsulated cargoes without destroying the vesicle integrity. The probe-loaded cationic fusogenic liposome could capture and fuse with a single target exosome, enabling probes delivery and target biomolecule-initiated cascaded signal amplification in situ. Then the DNAzyme probe encountered conformal change upon exosomal microRNA activation, and generated a convex DNAzyme structure to cleave the RNA site of substrate probe. After that, the target microRNA could be released to introduce a cleavage cycle to yield amplified fluorescence readout. Therefore, trace cargoes in a single exosome could be accurately determined by elaborately controlling the ratio of introduced LIFE probe, paving the way toward the exploration of a universal sensing platform for the assessment of exosomal cargoes to facilitate early disease diagnosis and personalized treatment.
- Published
- 2023
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