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Many bacteria have been proved to change physical (viscosity, wettability, and tension), and compositions of crude oil, which can make it easier for oil to be released from rock pores and achieve the purpose of improving recovery, which is called Microbial Enhanced Oil Recovery (MEOR). Our team has previously isolated six emulsified and viscosity-reducing bacteria (Bacillus. sp.) in low permeability layers (Chang 4+5 and Chang 6) of Ordos Basin. However, environmental tolerance of the strains is limited, and the components of crude oil used by the strains were also different. The combination of strains of different species and genera may enhance the effects of single bacteria, surpass the tolerance upper limit, and optimize the viscosity reduction and degradation. Therefore, in this study, it is extremely necessary to study the bacterial consortium. Two consortia were obtained and each consortium consisted of three bacterial strains and was designated as Consortium A (51+61, 61+H-1, 51+H-1; A-ALL) and Consortium B (34(2)+42, 34(2)+A-3; 42+A-3. B-BLL). The performance of the mixed strains was evaluated by the analysis of change in emulsification rate, crude oil composition, viscosity, and the tolerance (temperature, salinity, and pH) though GC-MS, rotational rheometer, and other methods. The results showed that bacterial consortiums had higher alkali resistance and could survive temperatures of 55 °C and salinity of 50 g/L in comparison to single bacterium. The emulsification rate was 22%-48%. Consortium B has better effects than Consortium A. The viscosity reduction rate of the Consortium A after 7 days was exceeded 30% as a whole, and the rate of Consortium B was more than 35%. The crude oil of Consortium B is basically non-stick to flask. Compared with single bacteria, the utilization components of crude oil to bacteria are still different, including both long chain hydrocarbons and short chain hydrocarbons. However, the proportion of long chain n-alkanes is further reduced compared with that of single bacteria, and the highest ratio is reduced by 23.81% (B-ALL). Overall, the bacterial consortium outperforms the single strain in terms of tolerance, viscosity reduction, and degradation, which further optimizes the application of MEOR.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to analyze the polymerization distribution of xylo-oligosaccharides from birchwood and beechwood xylans following hydrolysis by the xylanase (XynA) from Bacillus subtilis, which was obtained by recombinant expression and purified by Ni2+ affinity column chromatography. The hydrolysis products of birchwood and beechwood xylans were analyzed by thin layer chromatography (TLC) and MALDI-TOF-MS. In the birchwood hydrolysate, the main neutral xylo-oligosaccharides were xylobiose (X2 ) and xylotriose (X3 ), and the acidic xylo-oligosaccharides (polymerization degree 4-12) were attached via a single methyl-glucuronic acid sidechain (MeG). Both X2 and X3 were identified in the beechwood xylan hydrolysate and acidic xylo-oligosaccharides (polymerization degree 4-16), which were structurally similar to those in the birchwood xylan hydrolysate. Therefore, the recombinant xylanase, XynA, has the potential to produce xylobiose (X2 ) and xylotriose (X3 ) as well as acidic xylo-oligosaccharides (MeGXn ) that would be applied in food industry. PRACTICAL APPLICATIONS: Xylo-oligosaccharides are a novel functional food additive, which has great potential in improving the quality of food. Xylo-oligosaccharides with methyl-glucuronic acid sidechains (MeG) are acidic xylo-oligosaccharides (MeGXn), which can be applied in the preparation of drugs for the treatment of cystitis and mucopolysaccharidosis. In this study, xylanase XynA was first obtained by gene cloning and expression and then used to hydrolyze the birchwood and beechwood xylans. The polymerization distribution of xylo-oligosaccharides generated during the enzymatic digestion was then determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Through enzyme hydrolysis, we are able to produce xylobiose and xylotriose for food additives.

Highlights l COVID-19 mortality risk factors were similar to those of SARS and MERS. l Advanced age, hypertension, diabetes, chronic lung disease, increased LDH, CRP, neutrophils and BUN and decreased albumin were positively correlated with COVID-19 mortality. l The laboratory indicators of poor outcomes with the highest degrees of difference were similar among the three coronavirus diseases. l Low lymphocyte subtype counts might be a factor in COVID-19 mortality., SUMMARY Objective: Coronavirus Disease 2019 (COVID-19) is a pandemic. This systematic review compares mortality risk factors including clinical, demographic and laboratory features of COVID-19, Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). The aim is to provide new strategies for COVID-19 prevention and treatment. Methods: We performed a systematic review with meta-analysis, using five databases to compare the predictors of death for COVID-19, SARS and MERS. A random-effects model meta-analysis calculated odds ratios (OR) and 95% confidence intervals (95% CI). Results: 845 articles up through 11/4/2020 were retrieved, but only 28 studies were included in this meta-analysis. The results showed that males had a higher likelihood of death than females (OR = 1.82, 95% CI 1.56-2.13). Age (OR = 7.86, 95% CI 5.46-11.29), diabetes comorbidity (OR = 3.73, 95% CI 2.35-5.90), chronic lung disease (OR = 3.43, 95% CI 1.80-6.52) and hypertension (OR = 3.38, 95% CI 2.45-4.67) were the mortality risk factors. The laboratory indicators lactic dehydrogenase (OR = 37.52, 95% CI 24.68-57.03), C-reactive protein (OR = 12.11, 95% CI 5.24-27.98), and neutrophils (OR = 17.56, 95% CI 10.67-28.90) had stronger correlations with COVID-19 mortality than with SARS or MERS mortality. Consolidation and ground-glass opacity imaging features were similar among COVID-19, SARS, and MERS patients. Conclusions: COVID-19’s mortality factors are similar to those of SARS and MERS. Age and laboratory indicators could be effective predictors of COVID-19 mortality outcomes.

1,2-Dichloroethane (1,2-DCE) is a widely used chlorinated organic toxicant, but little is known about the cerebellar dysfunction induced by excessive exposure to it. To uncover 1,2-DCE-induced neurotoxicity in cerebellar granular cells (CGCs), and to investigate the underlying mechanisms, we explored this, both in vitro and in vivo. Our findings showed significant cell viability inhibition in human CGCs (HCGCs) treated with 1,2-DCE. Flow cytometry and mitochondrial membrane potential analyses discovered an increase in apoptotic-mediated cell death in HCGCs after 1,2-DCE treatment. This HCGC apoptosis was involved in the increases of protein expression in Cytochrome c, Caspase-3, Bad, Bim, transformation related protein 53, Caspase-8, tumor necrosis factor-α, and Survivin. Quantitative real-time PCR (qPCR) and western blot confirmed the increases in Cytochrome c, Caspase-3, cleaved Caspase-3, and Bad in HCGCs after 1,2-DCE treatment. Bax inhibitor peptide V5 rescued 1,2-DCE-induced HCGC apoptosis. Furthermore, 80 CD-1 male mice were exposed to 1,2-DCE by inhalation at 0, 100, 350, and 700 mg/m3 for 6 h/day for 4 weeks. An open field test found abnormal neurobehavioral changes in the mice exposed to 1,2-DCE. Histopathological examination showed significantly shrunken and hypereosinophilic cytoplasm with nuclear pyknosis in mouse CGCs from the 700 mg/m3 1,2-DCE group. TdT-mediated dUTP nick-end labeling assay verified significant increases in apoptotic positive cells in the mouse CGCs after 1,2-DCE exposure. We confirmed the increases in the expressions of Cytochrome c, Caspase-3, cleaved Caspase-3 and Bad in the mice exposed to 1,2-DCE. These findings suggest that 1,2-DCE exposure can induce CGC apoptosis and cerebellar dysfunction, at least in part, through mitochondrial pathway.