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The members of the genus Conamomum Ridl. (Zingiberaceae) are primarily distributed from Indochina to western Malesia. Knowledge of the distribution of species of Conamomum is important for conservation and natural resource management. The objective of this review is to comprehensively summarise the morphological characteristics, distribution, ecology, diversity, and potential pharmacological effects of Conamomum species worldwide. The current study also provides a scientific reference for future applications by comprehensively reviewing the literature from 1892 to 2024 via several scientific databases, such as Web of Science, Scopus, Google Scholar, PubMed, Science Direct, Springer, and other online databases. A cluster analysis conducted on 12 species of Conamomum provides valuable insights into their morphological similarities and distribution patterns. Conamomum cylindrostachys and C. flavidulum have anther crests that are horned and 2‐lobed, contrasting with the horned and 3‐lobed anther crests observed in other Conamomum species. Regarding distribution, three species – C. odorum, C. rubidum, and C. Vietnamense – are endemic to Vietnam, while C. spiceum and C. flavidulum are both endemic to Malaysia. A key to species of Conamomum based on the morphology is provided. Extracts and isolated compounds from Conamomum plants, mainly essential oils, have been assessed to possess numerous pharmacological properties, such as anti‐inflammatory, anti‐bacterial, anti‐fungal, anti‐larvicidal, and cytotoxic activities. This review provides valuable insights into the morphological, ecological, and pharmacological properties as well as the diversification of Conamomum and identifies the ecological adaptations specific to their habitat in this region. These findings are valuable resources for conservation efforts, sustainable utilization, and future studies. [ABSTRACT FROM AUTHOR]

This research investigates the Zingiberaceae family in Saraburi Province, Thailand, aiming to understand its biodiversity, distribution, ecology, conservation status, and traditional uses. Through systematic exploration, 103 species are identified, including a novel species, Kaempferia chaveerachiae Saensouk, P.Saensouk, and Boonma. Additionally, 23 species are newly reported for distribution in Saraburi Province. Methodologically, a district-wise analysis and the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis were employed to discern species prevalence and geographical patterns. Findings reveal varied species distribution aligned with diverse ecological conditions, informing targeted conservation strategies. Fourteen endemic species and the District Endemism Index emphasize localized conservation significance. Assessment of conservation status highlights critical and vulnerable species, prompting focused conservation initiatives. Phenological analysis underscores adaptability through flowering peaks tied to environmental cues. Ethnobotanical insights highlight the multifunctionality of Zingiberaceae, emphasizing its cultural significance and traditional applications. This study offers valuable insights into Zingiberaceae diversity, advocating for tailored conservation strategies and sustainable practices in Saraburi Province to safeguard its unique flora, habitats, and indigenous knowledge. [ABSTRACT FROM AUTHOR]

Abstract This research presents the discovery of two novel taxa, Curcuma sumonii Saensouk, P.Saensouk, Boonma & Techa, and C. wanchaii Saensouk, P.Saensouk, Maknoi & Boonma, belonging to the Curcuma subgenus Curcuma. Curcuma sumonii was collected in Sukhothai Province, Northern Thailand, while C. wanchaii was found in Kanchanaburi Province, Southwestern Thailand. Our study provides a comprehensive documentation of their morphological attributes, including detailed descriptions supported by visual aids. In addition to morphological data, we offer insights into vernacular names, ecological characteristics, phenology, traditional uses, conservation status, and distribution maps. Morphological comparison Tables 1 and 2 are provided to differentiate between these newly discovered taxa and their allied species. Furthermore, we present an identification key to facilitate the differentiation of these taxa from their allied species. The findings of this study contribute to the understanding of biodiversity within the Curcuma genus and offer valuable information for further conservation efforts and taxonomic research.

This study introduces Curcuma borealis Saensouk, P.Saensouk, and Boonma and C. retrocalcaria Saensouk, P.Saensouk, and Boonma, two newly discovered species within the Curcuma genus of the Zingiberaceae family, found in the Mae Hong Son and Chiang Mai Provinces of Northern Thailand, respectively. This research aims to clarify the taxonomic identity of these species, which were initially confused with C. ecomata Craib and C. chantaranothaii Boonma and Saensouk due to similarities in flower coloration and some morphological characteristics, respectively. Detailed morphological analysis was conducted to distinguish these species. Curcuma borealis is differentiated by its unique anthers lacking appendages or projections at the base of the spurs, in contrast to the appendages or projections present in C. ecomata, while C. retrocalcaria is characterized by anther spurs folding or curving backward under the anther base and with two purple patches at the base of the labellum, distinguishing it from C. chantaranothaii. The findings highlight distinct morphological traits that set C. borealis and C. retrocalcaria apart from existing Curcuma species. The pollen morphology of the two new taxa was also studied here for the first time. This research emphasizes the importance of thorough taxonomic scrutiny for accurate species identification and contributes to our understanding of the diversity within the Curcuma genus. Accurate identification of these species is crucial for conservation efforts, underscoring the need to protect their habitats and maintain biodiversity in the region. [ABSTRACT FROM AUTHOR]

A new species of Maranta from the Brazilian Atlantic Forest is described. The new species is most similar to Maranta ruiziana, from which it differs mainly in its bambusoid habit, non starch‐storing rhizome, homotropic leaves, with blade entirely glabrous on both surfaces and leaf margins entire, florescence components with numerous cymules, sepals longer than the corolla tube, and spheroidal fruit. The new species also presents morphological characters seen in Maranta tuberculata, from which is easily distinguished by its fruits. I characterize the new species by its external morphology, provide a distribution map, and perform a preliminary conservation assessment based on the IUCN criteria. [ABSTRACT FROM AUTHOR]

Family Zingiberaceae is well distributed in Peninsular Malaysia, including the southern region of Johor. A preliminary survey updated the current checklist of wild gingers from Panti Forest Reserve and Labis Forest Reserve. During the brief survey, 28 taxa from 8 genera were recorded. The genera include Amomum, Alpinia, Boesenbergia, Conamomum, Etlingera, Globba, Hornstedtia, Meistera, Scaphochlamys, Sundamomum, and Zingiber malaysianum. Three endemic species to Johor were recorded from the study sites: Scaphochlamys lanceolata, Scaphochlamys klossii var. glomerata, and Zingiber. [ABSTRACT FROM AUTHOR]

This research investigates the Zingiberaceae family in Saraburi Province, Thailand, aiming to understand its biodiversity, distribution, ecology, conservation status, and traditional uses. Through systematic exploration, 103 species are identified, including a novel species, Kaempferia chaveerachiae Saensouk, P.Saensouk, and Boonma. Additionally, 23 species are newly reported for distribution in Saraburi Province. Methodologically, a district-wise analysis and the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis were employed to discern species prevalence and geographical patterns. Findings reveal varied species distribution aligned with diverse ecological conditions, informing targeted conservation strategies. Fourteen endemic species and the District Endemism Index emphasize localized conservation significance. Assessment of conservation status highlights critical and vulnerable species, prompting focused conservation initiatives. Phenological analysis underscores adaptability through flowering peaks tied to environmental cues. Ethnobotanical insights highlight the multifunctionality of Zingiberaceae, emphasizing its cultural significance and traditional applications. This study offers valuable insights into Zingiberaceae diversity, advocating for tailored conservation strategies and sustainable practices in Saraburi Province to safeguard its unique flora, habitats, and indigenous knowledge.

This study introduces Curcuma borealis Saensouk, P.Saensouk, and Boonma and C. retrocalcaria Saensouk, P.Saensouk, and Boonma, two newly discovered species within the Curcuma genus of the Zingiberaceae family, found in the Mae Hong Son and Chiang Mai Provinces of Northern Thailand, respectively. This research aims to clarify the taxonomic identity of these species, which were initially confused with C. ecomata Craib and C. chantaranothaii Boonma and Saensouk due to similarities in flower coloration and some morphological characteristics, respectively. Detailed morphological analysis was conducted to distinguish these species. Curcuma borealis is differentiated by its unique anthers lacking appendages or projections at the base of the spurs, in contrast to the appendages or projections present in C. ecomata, while C. retrocalcaria is characterized by anther spurs folding or curving backward under the anther base and with two purple patches at the base of the labellum, distinguishing it from C. chantaranothaii. The findings highlight distinct morphological traits that set C. borealis and C. retrocalcaria apart from existing Curcuma species. The pollen morphology of the two new taxa was also studied here for the first time. This research emphasizes the importance of thorough taxonomic scrutiny for accurate species identification and contributes to our understanding of the diversity within the Curcuma genus. Accurate identification of these species is crucial for conservation efforts, underscoring the need to protect their habitats and maintain biodiversity in the region.

Curcuma ubonensis Boonma, Saensouk, Maknoi & P. Saensouk sp. nov. (Zingiberaceae) was recently discovered in Ubon Ratchathani Province, Eastern Thailand. Specimen identification involves comparing their morphology with descriptions in similar species' protologues. We performed measurements on specimens collected from their natural habitats, both living and preserved. Despite its resemblances with Curcuma prasina Škorničk. in having greenish inflorescence with small purple flowers that lack of epigynous glands, yet this novel species displays distinct features of a brownish-red margin of petiole (vs. C. prasina has green margin), lamina elliptic, 3.8-7.0 cm wide, margin brownish red (vs. narrowly elliptic, 1.5-3.2 cm wide, margin green); coma bract present (vs. absent), fertile bracts uniquely triangular-obovate, apex rounded (vs. almost orbicular, apex acute), and labellum reddish purple with four white stripes by two stripes rarely pale yellow extending from the basal to half of the sinus, and another two stripes from the basal along the longitudinally of each lobe to half the length of the lobe, with yellow at the middle groove of the labellum almost to the base (vs. purple with a bright yellow and swollen median band composed of two central lines extending from the base to about two-thirds the length of the labellum towards the apex, sides of the labellum with paler lines radiating towards the margin). Meticulous examination and morphological comparison of Curcuma ubonensis with related species within the Curcuma genus reveals its novelty. We comprehensively accounted for its morphological traits, including illustrations, vernacular name, ecological preferences, phenology, distribution, traditional uses, conservation status, distribution map, and a comparative table to its ally species. Furthermore, we present an updated identification key for differentiating species within the Curcuma subgenus Hitcheniopsis in Thailand. [ABSTRACT FROM AUTHOR]

Zingiberales includes eight families and more than 2600 species, with many species having important economic and ecological value. However, the backbone phylogenetic relationships of Zingiberales still remain controversial, as demonstrated in previous studies, and molecular dating based on chloroplast genomes has not been comprehensively studied for the whole order. Herein, 22 complete chloroplast genomes from 21 species in Zingiberales were sequenced, assembled, and analyzed. These 22 genomes displayed typical quadripartite structures, which ranged from 161,303 bp to 163,979 bp in length and contained 111–112 different genes. The genome structures, gene contents, simple sequence repeats, long repeats, and codon usage were highly conserved, with slight differences among these genomes. Further comparative analysis of the 111 complete chloroplast genomes of Zingiberales, including 22 newly sequenced ones and the remaining ones from the national center for biotechnology information (NCBI) database, identified three highly divergent regions comprising ccsA, psaC, and psaC-ndhE. Maximum likelihood and Bayesian inference phylogenetic analyses based on chloroplast genome sequences found identical topological structures and identified a strongly supported backbone of phylogenetic relationships. Cannaceae was sister to Marantaceae, forming a clade that was collectively sister to the clade of (Costaceae, Zingiberaceae) with strong support (bootstrap (BS) = 100%, and posterior probability (PP) = 0.99–1.0); Heliconiaceae was sister to the clade of (Lowiaceae, Strelitziaceae), then collectively sister to Musaceae with strong support (BS = 94–100%, and PP = 0.93–1.0); the clade of ((Cannaceae, Marantaceae), (Costaceae, Zingiberaceae)) was sister to the clade of (Musaceae, (Heliconiaceae, (Lowiaceae, Strelitziaceae))) with robust support (BS = 100%, and PP = 1.0). The results of divergence time estimation of Zingiberales indicated that the crown node of Zingiberales occurred approximately 85.0 Mya (95% highest posterior density (HPD) = 81.6–89.3 million years ago (Mya)), with major family-level lineages becoming from 46.8 to 80.5 Mya. These findings proved that chloroplast genomes could contribute to the study of phylogenetic relationships and molecular dating in Zingiberales, as well as provide potential molecular markers for further taxonomic and phylogenetic studies of Zingiberales. [ABSTRACT FROM AUTHOR]

Curcuma ubonensis Boonma, Saensouk, Maknoi & P. Saensouk sp. nov. (Zingiberaceae) was recently discovered in Ubon Ratchathani Province, Eastern Thailand. Specimen identification involves comparing their morphology with descriptions in similar species' protologues. We performed measurements on specimens collected from their natural habitats, both living and preserved. Despite its resemblances with Curcuma prasina Škorničk. in having greenish inflorescence with small purple flowers that lack of epigynous glands, yet this novel species displays distinct features of a brownish-red margin of petiole (vs. C. prasina has green margin), lamina elliptic, 3.8-7.0 cm wide, margin brownish red (vs. narrowly elliptic, 1.5-3.2 cm wide, margin green); coma bract present (vs. absent), fertile bracts uniquely triangular-obovate, apex rounded (vs. almost orbicular, apex acute), and labellum reddish purple with four white stripes by two stripes rarely pale yellow extending from the basal to half of the sinus, and another two stripes from the basal along the longitudinally of each lobe to half the length of the lobe, with yellow at the middle groove of the labellum almost to the base (vs. purple with a bright yellow and swollen median band composed of two central lines extending from the base to about two-thirds the length of the labellum towards the apex, sides of the labellum with paler lines radiating towards the margin). Meticulous examination and morphological comparison of Curcuma ubonensis with related species within the Curcuma genus reveals its novelty. We comprehensively accounted for its morphological traits, including illustrations, vernacular name, ecological preferences, phenology, distribution, traditional uses, conservation status, distribution map, and a comparative table to its ally species. Furthermore, we present an updated identification key for differentiating species within the Curcuma subgenus Hitcheniopsis in Thailand.

We present a taxonomic treatment for the monospecific genus Koernickanthe (Marantaceae) based on morphology and previous phylogenetic studies. We return K. orbiculata to Maranta once more and have located the last remnant syntype for its basionym, Ischnosiphon orbiculatus, deposited at the Komarov Botanical Institute of RAS herbarium (LE), designating it as lectotype. Nomenclature and taxonomic novelties include the acceptance of the name Maranta orbiculata and its correct application, and also provide lectotypification for its basionym and that of its synonym, Calathea cardenasii. [ABSTRACT FROM AUTHOR]

The paper deals with the seed morphology and anatomy of 32 wild Indian Musaceae by using light microscopy (LM) and scanning electron microscopy (SEM). This is the first comprehensive investigation into the seed micro-morphological and anatomical aspects of two genera of the family. Seed characters including shape, size, surface sculpturing (LM and SEM), seed coat thickness, layer characters and chalazal chamber column were considered. Seed characters play a significant role in the delimitation of taxa as they are more stable than vegetative (anatomical) and pollen features. Four types of seeds (oblate, ovoid, sub-globose and ellipsoid) were observed in the family, oblate seeds being the most common. LM revealed that most of the seeds have verrucate surface sculpturing, but some are smooth-verrucate or smooth. Seed size also plays an important role in the identification of the taxa to some extent. Number of layers and seed coat thickness are directly proportional to seed size. However, the thickness of endotesta, tegmen and inner endosperm layers (aleurone layers) differ in small and large seeds. The present study revealed eight types of outer sculpturing and three inner sculpturing patterns with diverse nature of anticlinal and periclinal cell walls. Based on the seed characters, a phenetic analysis was also carried out. [ABSTRACT FROM AUTHOR]

Floral symmetry studies often focus on the development of monosymmetric and polysymmetric flowers, whereas asymmetric flowers and their position and function within the inflorescence structure are largely neglected. Cannaceae is one of the few families that possesses truly asymmetric flowers, serving as a model to study the characters and mechanisms involved in the development of floral asymmetry and its context within the developing and mature inflorescence. In this study, inflorescence structure and floral morphology of normal asymmetric flowers and 16 aberrant flower collections from Canna indica L. and C. glauca L. were photographed, analyzed, and compared with attention to stamen petaloidy, floral symmetry, and inflorescence branching patterns anterior and posterior to the aberrant flower. In comparison with normal flowers, the aberrant flowers are arranged into abnormal partial florescences, and vary in floral symmetry, orientation, and degree of androecial petaloidy. The appendage of the fertile stamen is universally located distal from the higher order bract, indicating an underlying influence of inflorescence architecture. A synthetic model is proposed to explain the relationship between floral symmetry and inflorescence structure. Data from the observation of aberrant phenotypes strongly support the hypothesis that irregular petaloidy of the stamens is correlated with an asymmetric morphogenetic field within the inflorescence that contributes to the overall floral asymmetry in Canna flowers. [ABSTRACT FROM AUTHOR]

Selection together with variation in floral traits can act to mold floral form, often driven by a plant's predominant or most effective pollinators. To investigate the evolution of traits associated with pollination, we developed a phylogenetic framework for evaluating tempo and mode of pollination shifts across the genus Costus L., known for its evolutionary toggle between traits related to bee and bird pollination. Using a target enrichment approach, we obtained 957 loci for 171 accessions to expand the phylogenetic sampling of Neotropical Costus. In addition, we performed whole genome resequencing for a subset of 20 closely related species with contrasting pollination syndromes. For each of these 20 genomes, a high-quality assembled transcriptome was used as reference for consensus calling of candidate loci hypothesized to be associated with pollination-related traits of interest. To test for the role these candidate genes may play in evolutionary shifts in pollinators, signatures of selection were estimated as dN/dS across the identified candidate loci. We obtained a well-resolved phylogeny for Neotropical Costus despite conflict among gene trees that provide evidence of incomplete lineage sorting and/or reticulation. The overall topology and the network of genome-wide single nucleotide polymorphisms (SNPs) indicate that multiple shifts in pollination strategy have occurred across Costus, while also suggesting the presence of previously undetected signatures of hybridization between distantly related taxa. Traits related to pollination syndromes are strongly correlated and have been gained and lost in concert several times throughout the evolution of the genus. The presence of bract appendages is correlated with two traits associated with defenses against herbivory. Although labellum shape is strongly correlated with overall pollination syndrome, we found no significant impact of labellum shape on diversification rates. Evidence suggests an interplay of pollination success with other selective pressures shaping the evolution of the Costus inflorescence. Although most of the loci used for phylogenetic inference appear to be under purifying selection, many candidate genes associated with functional traits show evidence of being under positive selection. Together these results indicate an interplay of phylogenetic history with adaptive evolution leading to the diversification of pollination-associated traits in Neotropical Costus. [ABSTRACT FROM AUTHOR]

Selection together with variation in floral traits can act to mold floral form, often driven by a plant’s predominant or most effective pollinators. To investigate the evolution of traits associated with pollination, we developed a phylogenetic framework for evaluating tempo and mode of pollination shifts across the genus Costus L., known for its evolutionary toggle between traits related to bee and bird pollination. Using a target enrichment approach, we obtained 957 loci for 171 accessions to expand the phylogenetic sampling of Neotropical Costus. In addition, we performed whole genome resequencing for a subset of 20 closely related species with contrasting pollination syndromes. For each of these 20 genomes, a high-quality assembled transcriptome was used as reference for consensus calling of candidate loci hypothesized to be associated with pollination-related traits of interest. To test for the role these candidate genes may play in evolutionary shifts in pollinators, signatures of selection were estimated as dN/dS across the identified candidate loci. We obtained a well-resolved phylogeny for Neotropical Costus despite conflict among gene trees that provide evidence of incomplete lineage sorting and/or reticulation. The overall topology and the network of genome-wide single nucleotide polymorphisms (SNPs) indicate that multiple shifts in pollination strategy have occurred across Costus, while also suggesting the presence of previously undetected signatures of hybridization between distantly related taxa. Traits related to pollination syndromes are strongly correlated and have been gained and lost in concert several times throughout the evolution of the genus. The presence of bract appendages is correlated with two traits associated with defenses against herbivory. Although labellum shape is strongly correlated with overall pollination syndrome, we found no significant impact of labellum shape on diversification rates. Evidence suggests an interplay of pollination success with other selective pressures shaping the evolution of the Costus inflorescence. Although most of the loci used for phylogenetic inference appear to be under purifying selection, many candidate genes associated with functional traits show evidence of being under positive selection. Together these results indicate an interplay of phylogenetic history with adaptive evolution leading to the diversification of pollination-associated traits in Neotropical Costus.

Mount HamiguitanRange Wildlife Sanctuary is a center of botanical diversity throughout the Philippine archipelago. However, as to how many Zingiberaceae species are present in the area is still poorly known. Thus, floristic surveys were made and collections were conducted from July 2016 to September 2018. In total, 18 species belonging to eight genera representing three tribes of the family were recorded. The most species rich tribe belongs to Alpinieae with 14 species in 6 genera. This study presents an annotated checklist of Zingiberaceae species found in the sanctuary which is beneficial for its efficient biological resource manage-ment

El Hedychium coronarium Koen. es una planta muy interesante que tiene gran aplicación en la industria brasileña del papel y también en la preparación de almidón (araruta) utilizado para cocciones finas. Este trabajo contiene esencialmente una contribución al conocimiento de la anatomía del rizoma y de la estructura del almidón.

Leaf epidermal characteristics are important for phylogenetic and taxonomic studies of many plants, but there is currently insufficient such data for this application in Zingiber species. Therefore, the leaf epidermal micromorphology of 22 species in three sections of Zingiber was investigated by light microscopy and scanning electron microscopy. Differences between various taxonomic groups of Zingiberaceae were also compared to assess their phylogenetic and taxonomic significance. As in other genera of Zingiberaceae, the epidermal cells in both the adaxial and abaxial epidermis of Zingiber species were found to be hexagonal or polygonal, with non-sinuous anticlinal walls that are arranged parallel to leaf veins. Tetracytic stomata are mostly randomly distributed in the intercostal regions of both surfaces and are more common on the abaxial surface. The stomatal density of the species in sect. Pleuranthesis is significantly lower than that in sects. Zingiber and Cryptanthium. There are two types of trichome in Zingiber: so-called "delicate" trichomes are present in most species, while "stout" trichomes with a swollen base are only found in Z. corallinum and Z. montanum. Oil cells occur in both epidermal layers of some species in sects. Zingiber and Cryptanthium, but only in the abaxial epidermis of Z. ellipticum in sect. Pleuranthesis. Crystals are found in the abaxial epidermis only in all species, but are present in both epidermal layers of Z. corallinum and Z. montanum. Although the epidermal morphology is similar in most Zingiber species, stomatal density, type of trichome and distribution of oil cells and crystals offer valuable information for the systematic and taxonomic studies in this genus. [ABSTRACT FROM AUTHOR]

A new species, Globba philippinensis Naive, J.A.G. Dalisay, Bangcaya & Sangvir. from Antique Province, Philippines, is herein described and illustrated. A detailed description, colour plates, discussion of similar taxa, information on its distribution and habitat are provided. A preliminary conservation assessment is proposed and a key to the species of Globba section Nudae subsection Mediocalcaratae is amended. [ABSTRACT FROM AUTHOR]

Questions: Understanding spatial variation in floristic composition is crucial to quantify the extent, patchiness and connectivity of distinct habitats and their spatial relationships. Broad‐scale variation in floristic composition and the degree of uniqueness of different regions remains poorly mapped and understood in several areas across the globe. We here aim to map vegetation heterogeneity in Amazonia. Location: Middle Juruá river region, Amazonas State, Brazil. Methods: We mapped four plant groups by applying machine learning to scale up locally observed community composition and using environmental and remotely sensed variables as predictors, which were obtained as GIS layers. To quantify how reliable our predictions were, we made an assessment of model transferability and spatial applicability. We also compared our floristic composition map to the official Brazilian national‐level vegetation classification. Results: The overall performance of our floristic models was high for all four plant groups, especially ferns, and the predictions were found to be spatially congruent and highly transferable in space. For some areas, the models were assessed not to be applicable, as the field sampling did not cover the spectral or environmental characteristics of those regions. Our maps show extensive habitat heterogeneity across the region. When compared to the Brazilian vegetation classification, floristic composition was relatively homogeneous within dense forests, while floristic heterogeneity in rainforests classified as open was high. Conclusion: Our maps provide geoecological characterization of the regions and can be used to test biogeographical hypotheses, develop species distribution models and, ultimately, aid science‐based conservation and land‐use planning. [ABSTRACT FROM AUTHOR]

The Zingiberales is an order of tropical monocots that exhibits diverse floral morphologies. The evolution of petaloid, laminar stamens, staminodes, and styles contributes to this diversity. The laminar style is a derived trait in the family Cannaceae and plays an important role in pollination as its surface is used for secondary pollen presentation. Previous work in the Zingiberales has implicated YABBY2-like genes, which function in promoting laminar outgrowth, in the evolution of stamen morphology. Here, we investigate the evolution and expression of Zingiberales YABBY2-like genes in order to understand the evolution of the laminar style in Canna. Phylogenetic analyses show that multiple duplication events have occurred in this gene lineage prior to the diversification of the Zingiberales. Reverse transcription-PCR in Canna, Costus, and Musa reveals differential expression across floral organs, taxa, and gene copies, and a role for YABBY2-like genes in the evolution of the laminar style is proposed. Selection tests indicate that almost all sites in conserved domains are under purifying selection, consistent with their functional relevance, and a motif unique to monocot YABBY2-like genes is identified. These results contribute to our understanding of the molecular mechanisms underlying the evolution of floral morphologies.

Floral symmetry studies often focus on the development of monosymmetric and polysymmetric flowers, whereas asymmetric flowers and their position and function within the inflorescence structure are largely neglected. Cannaceae is one of the few families that possesses truly asymmetric flowers, serving as a model to study the characters and mechanisms involved in the development of floral asymmetry and its context within the developing and mature inflorescence. In this study, inflorescence structure and floral morphology of normal asymmetric flowers and 16 aberrant flower collections from Canna indica L. and C. glauca L. were photographed, analyzed, and compared with attention to stamen petaloidy, floral symmetry, and inflorescence branching patterns anterior and posterior to the aberrant flower. In comparison with normal flowers, the aberrant flowers are arranged into abnormal partial florescences, and vary in floral symmetry, orientation, and degree of androecial petaloidy. The appendage of the fertile stamen is universally located distal from the higher order bract, indicating an underlying influence of inflorescence architecture. A synthetic model is proposed to explain the relationship between floral symmetry and inflorescence structure. Data from the observation of aberrant phenotypes strongly support the hypothesis that irregular petaloidy of the stamens is correlated with an asymmetric morphogenetic field within the inflorescence that contributes to the overall floral asymmetry in Canna flowers.

Canna indica is a common ornamental plant with asymmetric flowers having colourful petaloid staminodes. The only fertile stamen comprises a one‐theca anther and a petaloid appendage and represents the lowest stamen number in the order Zingiberales. The molecular mechanism for the asymmetric androecial petaloidy remains poorly understood. Here, we studied the identity specification in Canna stamen.We observed four types of abnormal flower in terms of androecium identity transformation and analysed the corresponding floral symmetry changes. We further tested the expression patterns of B‐ and C‐class MADS‐box genes using in situ hybridization in normal Canna stamen.Homeotic conversions in the androecium were accompanied by floral symmetry changes, and the asymmetric stamen is key in contributing to the floral asymmetry. Both B‐ and C‐class genes exhibited higher expression levels in the anther primordium than in other androecial parts. This asymmetric expression pattern precisely corresponded to the asymmetric identities of the Canna androecium.We identified C. indica as a model species for studying androecial organ identity and floral symmetry synthetically in Zingiberales. We hypothesized that homeotic genes specify floral organ identity in a putative dose‐dependent manner. The results add to the current understanding of organ identity‐related floral symmetry. [ABSTRACT FROM AUTHOR]

This study summarizes the taxonomy of the species of Maranta from northeastern Brazil. While 20 names have been proposed in the region, only 13 are accepted. We provide two new records, M. rugosa and M. polystachya, and five new species are described, of which M. bahiensis and M. villosovaginata are endemic to Bahia, M. chrysogina to Ceará, M. vieirae to Maranhão, and M. lorifolia occurs in Bahia and Espírito Santo. Maranta noctiflora, M. parvifolia, M. phrynioides, and M. rupicola L. have distributions outside of the study area, while M. anderssoniana and M. hatschbachiana are synonymized. Our final checklist includes 21 species, which represent more than half of the species in the genus, highlighting northeastern Brazil as a center of diversity for Maranta. Among the species listed, M. lorifolia, M. tuberculata, and M. zingiberina are thought to be endangered (EN), while M. gigantea is likely critically endangered (CR). Finally, this study provides informal conservation statuses, descriptions, distribution maps, and an identification key to the species. Typifications for M. subterranea and M. polystachya are also provided. [ABSTRACT FROM AUTHOR]

In a typical cincinnus, the neighboring two flowers are generally enantiomorphic, which leads to the pendulum symmetry of the entire cyme. While in a two-flowered Cannaceae cincinnus, the flowers develop the same chirality. In this study, we observed several abnormal cincinni of Canna indica that extended longer than their normal form, which presented a second enantiomorphic flower, thus reflecting a typical pendulum symmetry. The chirality change of the second flower was strongly associated with the position of the lateral cincinnus meristem, which determines the angle size of the cincinnus zigzag shift and may serve as a key factor controlling the formation of pendulum symmetry. We propose that alternating floral chirality and the concomitant pendulum symmetry are the essential characteristics of a typical cincinnus. Accordingly, Canna flowers with the same chirality are arranged in modified cincinni. [ABSTRACT FROM AUTHOR]

The asymmetric flower, lacking any plane of symmetry, is rare among angiosperms. Canna indica L. has conspicuously asymmetric flowers resulting from the presence of a half-fertile stamen, while the other androecial members develop as petaloid staminodes or abort early during development. The molecular basis of the asymmetric distribution of fertility and petaloidy in the androecial whorls remains unknown. Ontogenetic studies have shown that Canna flowers are borne on monochasial (cincinnus) partial florescences within a racemose inflorescence, with floral asymmetry likely corresponding to the inflorescence architecture. Given the hypothesized role of CYC/TB1 genes in establishing floral symmetry in response to the influence of the underlying inflorescence architecture, the spatiotemporal expression patterns of three Canna CYC/TB1 homologs (CiTBL1a, CiTBL1b-1, and CiTBL1b-2) were analyzed during inflorescence and floral development using RNA in situ hybridization and qRT-PCR. In the young inflorescence, both CiTBL1a and CiTBL1b-1 were found to be expressed in the bracts and at the base of the lateral florescence branches, whereas transcripts of CiTBL1b-2 were mainly detected in flower primordia and inflorescence primordia. During early flower development, expression of CiTBL1a and CiTBL1b-1 were both restricted to the developing sepals and petals. In later flower development, expression of CiTBL1a was reduced to a very low level while CiTBL1b-1 was detected with extremely high expression levels in the petaloid androecial structures including the petaloid staminodes, the labellum, and the petaloid appendage of the fertile stamen. In contrast, expression of CiTBL1b-2 was strongest in the fertile stamen throughout flower development, from early initiation of the stamen primordium to maturity of the ½ anther. Heterologous overexpression of CiTBL genes in Arabidopsis led to dwarf plants with smaller petals and fewer stamens, and altered the symmetry of mature flowers. These data provide evidence for the involvement of CYC/TB1 homologs in the development of the asymmetric Cannaceae flower.

Mount Hamiguitan Range Wildlife Sanctuary is a center of botanical diversity throughout the Philippine archipelago. However, as to how many Zingiberaceae species are present in the area is still poorly known. Thus, floristic surveys were made and collections were conducted from July 2016 to September 2018. In total, 18 species belonging to eight genera representing three tribes of the family were recorded. The most species rich tribe belongs to Alpinieae with 14 species in 6 genera. This study presents an annotated checklist of Zingiberaceae species found in the sanctuary which is beneficial for its efficient biological resource management. [ABSTRACT FROM AUTHOR]

Canna indica L. has been traditionally used to treat various diseases. Based on previously reported antithrombotic effect for this plant, two new phenylpropanoid sucrose esters (canindicoside A ( 1 ) and canindicoside B ( 2 )) and seven known compounds: nepetoidin B ( 3) , caffeic acid ( 4 ), ferulic acid ( 5 ), ( R) -(+)-rosmarinic acid ( 6 ), isorinic acid ( 7 ), ( S) -(-)-rosmarinic acid ( 8 ) and ( S )-(-)-rosmarinic acid methyl ester ( 9 ) were isolated from the ethyl acetate extract. Compounds were elucidated by NMR and MS spectroscopic methods. The antiplatelet effect was evaluated using turbidimetric method. Anticoagulant activity was examined by measuring activated partial thromboplastine time (APTT), prothrombin time, and thrombine time (TT). It was shown for the first time that both new phenylpropanoid sucrose esters 1 and 2 , 7 and 9 displayed dose-dependent antiplatelet effects. 2 and 9 had the highest inhibitory activity on both adenosine diphosphate (ADP)- and collagen-induced platelet aggregation. Moreover, 1 , 7 and 9 also exhibited anticoagulant activity. At 0.4 mg/mL, both 1 and 7 prolonged APTT compared to the negative control ( p  < 0.05), suggesting the possible inhibitory impact on the intrinsic coagulation pathway. Moreover, 9 at 0.4 mg/mL exerted higher TT values than the negative control ( p  < 0.05). C. indica and its bioactive phytochemicals are potential candidates for development of anti-thrombosis therapy.

The asymmetric flower, lacking any plane of symmetry, is rare among angiosperms. Canna indica L. has conspicuously asymmetric flowers resulting from the presence of a half-fertile stamen, while the other androecial members develop as petaloid staminodes or abort early during development. The molecular basis of the asymmetric distribution of fertility and petaloidy in the androecial whorls remains unknown. Ontogenetic studies have shown that Canna flowers are borne on monochasial (cincinnus) partial florescences within a racemose inflorescence, with floral asymmetry likely corresponding to the inflorescence architecture. Given the hypothesized role of CYC / TB1 genes in establishing floral symmetry in response to the influence of the underlying inflorescence architecture, the spatiotemporal expression patterns of three Canna CYC / TB1 homologs (CiTBL1a , CiTBL1b-1 , and CiTBL1b-2) were analyzed during inflorescence and floral development using RNA in situ hybridization and qRT-PCR. In the young inflorescence, both CiTBL1a and CiTBL1b-1 were found to be expressed in the bracts and at the base of the lateral florescence branches, whereas transcripts of CiTBL1b-2 were mainly detected in flower primordia and inflorescence primordia. During early flower development, expression of CiTBL1a and CiTBL1b-1 were both restricted to the developing sepals and petals. In later flower development, expression of CiTBL1a was reduced to a very low level while CiTBL1b-1 was detected with extremely high expression levels in the petaloid androecial structures including the petaloid staminodes, the labellum, and the petaloid appendage of the fertile stamen. In contrast, expression of CiTBL1b-2 was strongest in the fertile stamen throughout flower development, from early initiation of the stamen primordium to maturity of the ½ anther. Heterologous overexpression of CiTBL genes in Arabidopsis led to dwarf plants with smaller petals and fewer stamens, and altered the symmetry of mature flowers. These data provide evidence for the involvement of CYC / TB1 homologs in the development of the asymmetric Cannaceae flower. [ABSTRACT FROM AUTHOR]

Recognition and delimitation of taxonomic categories of biological organisms are still challenging and full of controversy. We used Ischnosiphon as a model to unravel the importance of morphometrics as individual-based variables to disentangle the morphological variability of plant species. Ischnosiphon spp. continue to be problematic for users, taxonomists and ecologists, due mainly to the huge morphological variability, the species criteria and circumscription proposed for many taxa and the many habitat and vegetative macro-morphological characters lacking in most currently available exsiccates. Twenty-three morphometric variables were sampled from 228 individuals, belonging to 22 Ischnosiphon spp. Principal components and discriminant multivariate analyses were used to describe and identify patterns of morphological variation in Ischnosiphon. Individual-landmark assessment analysed with multivariate methods captured morphometric intraspecific diversity and morphological variability in Ischnosiphon spp. along with the continuous variation of important morphological traits. By examining the morphology of Ischnosiphon spp. through individual-landmark assessment, we demonstrate that different morphological species concepts used today in the identification of the species are difficult to apply. We propose a replicable and analytical framework to accommodate individual variability in species diagnosis in morphologically diverse plant groups. [ABSTRACT FROM AUTHOR]

The family of pantropical spiral gingers (Costaceae Nakai; c. 125 spp.) can be used as a model to enhance our understanding of the mechanisms underlying Neotropical diversity. Costaceae has higher taxonomic diversity in South and Central America (c. 72 Neotropical species, c. 30 African, c. 23 Southeast Asian), particularly due to a radiation of Neotropical species of the genus Costus L. (c. 57 spp.). However, a well-supported phylogeny of the Neotropical spiral gingers including thorough sampling of proposed species encompassing their full morphologic and geographic variation is lacking, partly due to poor resolution recovered in previous analyses using a small sampling of loci. Here we use a phylogenomic approach to estimate the phylogeny of a sample of Neotropical Costus species using a targeted enrichment approach. Baits were designed to capture conserved elements’ variable at the species level using available genomic sequences of Costus species and relatives. We obtained 832 loci (generating 791,954 aligned base pairs and 31,142 parsimony informative sites) for samples that encompassed the geographical and/or morphological diversity of some recognized species. Higher support values that improve the results of previous studies were obtained when including all the available loci, even those producing unresolved gene trees and having a low proportion of variable sites. Concatenation and coalescent-based species trees methods converge in almost the same topology suggesting a robust estimation of the relationships, even under the high levels of gene tree conflict presented here. The bait set design here presented made inferring a robust phylogeny to test taxonomic hypotheses possible and will improve our understanding of the origins of the charismatic diversity of the Neotropical spiral gingers.

In flowering plants, lateral organs including stamens develop according to the precise regulation of adaxial–abaxial polarity. However, the polarity establishment process is poorly understood in asymmetric stamens. Canna indica (Zingiberales: Cannaceae) is a common ornamental plant with an asymmetric stamen comprising a one-theca anther and a petaloid appendage. In this study, we depicted the monosymmetric-to-asymmetric morphogenesis of C. indica stamen, and the morphogenesis of the monosymmetric stamen of a sister species was used as a contrast. We chose a HD-ZIP III gene family member and a YABBY family member as the adaxial and abaxial polarity marker genes, respectively, and tested their expression using mRNA in situ hybridization. The expression patterns of the two genes changed dynamically and asymmetrically during the stamen development process. Compared with their homologues in Arabidopsis thaliana , these two genes exhibited some specific expression patterns. We hypothesize that the distinctive adaxial–abaxial polarity participates in the irregular morphogenesis of C. indica stamen, which mediates the putative stamen-to-petaloid staminode conversion in this species. [ABSTRACT FROM AUTHOR]

The family of pantropical spiral gingers (Costaceae Nakai; c. 125 spp.) can be used as a model to enhance our understanding of the mechanisms underlying Neotropical diversity. Costaceae has higher taxonomic diversity in South and Central America (c. 72 Neotropical species, c. 30 African, c. 23 Southeast Asian), particularly due to a radiation of Neotropical species of the genus Costus L. (c. 57 spp.). However, a well-supported phylogeny of the Neotropical spiral gingers including thorough sampling of proposed species encompassing their full morphologic and geographic variation is lacking, partly due to poor resolution recovered in previous analyses using a small sampling of loci. Here we use a phylogenomic approach to estimate the phylogeny of a sample of Neotropical Costus species using a targeted enrichment approach. Baits were designed to capture conserved elements' variable at the species level using available genomic sequences of Costus species and relatives. We obtained 832 loci (generating 791,954 aligned base pairs and 31,142 parsimony informative sites) for samples that encompassed the geographical and/or morphological diversity of some recognized species. Higher support values that improve the results of previous studies were obtained when including all the available loci, even those producing unresolved gene trees and having a low proportion of variable sites. Concatenation and coalescent-based species trees methods converge in almost the same topology suggesting a robust estimation of the relationships, even under the high levels of gene tree conflict presented here. The bait set design here presented made inferring a robust phylogeny to test taxonomic hypotheses possible and will improve our understanding of the origins of the charismatic diversity of the Neotropical spiral gingers. [ABSTRACT FROM AUTHOR]

The knowledge on the use of plants of the order Zingiberales by the Malaipandaram tribe inhabited in South India was documented. The data was recorded through questionnaires after proper consultation with the traditional healers and others. The informant consensus factor and use value were analysed. Taxonomic studies were carried out and herbarium specimens were preserved at Catholic Volege Herbarius (CATH) herbarium and live specimens were conserved in the Catholicate College Botanical Garden. A total of 17 ethnobotanically important species were identified in Zingiberales distributed under 5 families, viz., Zingiberaceae, Costaceae, Musaceae, Marantaceae and Cannaceae. The plants were listed with scientific name, local name, family, parts used, preparation methods and use. The commonly used taxa was Curcuma longa with 52 use reports and highest use value of 1.62. In the investigation, endocrinal disorders and tooth pain reported highest Fic of 1. The information collected will be the baseline data for future phytochemical and pharmacological research to develop new drugs and service. [ABSTRACT FROM AUTHOR]

Marantaceae consist of species with asymmetric leaves of two types: those with either a wider left or right half; this asymmetry is related, respectively, to clockwise or counterclockwise convolute vernation. In this study, we analysed whether anatomical differences in the leaf edges, i.e. the anatomical asymmetry, were related to the orientation of the convolute vernation and to the asymmetry of leaf morphology, and whether these differences supported the organization of the clades in the family. Transverse sections of the mid third of the leaf buds expanded to the height of the right and left edges of the blades were prepared for 19 species belonging to 11 genera, using cyto-histological techniques. Anatomical analyses of the blade edges revealed that there is a relationship between morphological asymmetry and anatomical asymmetry that has never before been ascribed to the family. The anatomical data support differences between the arrangements in two of the three Neotropical informal groups. In the Calathea clade, Calathea showed much more similarity with Goeppertia than with Ischnosiphon and Monotagma , since they are the only genera that do not present with anatomical asymmetry. In the Maranta clade, Ctenanthe , Saranthe and Stromanthe appear to be related to one another, as they share strong anatomical asymmetry and fibrous edges. These characteristics, however, are not observed in Myrosma , which in turn is more anatomically similar to Maranta. [ABSTRACT FROM AUTHOR]

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Sixteen species of Costus s.s. are cited to Brazil, all of which can be found in the Brazilian Amazon, while only three also grow in the Atlantic Forest: C. arabicus, C. scaber, and C. spiralis. Studies of the genus from the Brazilian Atlantic Forest evidenced a distinctive fourth taxon, similar overall to C. spiralis, which is an ornithophilous species, however the flower morphology of this new taxon indicates it to be melittophilous. Hybridization and polyploidy (allopolyploidy or autopolyploidy) may play a role as an important source for evolutionary events, especially in recent speciose lineages such as Neotropical Costus. Here we investigated the evolutionary origin of this interesting Costus species using an integrative approach. This approach comprised a morphological analysis, cytogenetic analyses (chromosome counts, CMA/DAPI staining, and fluorescent in situ hybridization), genome size estimations, and a molecular phylogenetic reconstruction (using the plastid regions rps16 and trnL-F, and the nuclear ITS and ETS), aiming to answer the following questions: is it a hybrid?; is it an autopolyploid of C. spiralis?; or, if it is a different species, is it even phylogenetically related to C. spiralis or C. arabicus? The studies performed did not indicate any evidence of polyploidy, however, a chromosomal polymorphism was observed between the populations of the taxon studied. The phylogenetic position of this peculiar taxon with C. erythrophyllus, C. spiralis, and C. varzearum also excludes the putative partial heritage of C. arabicus which had been previously hypothesized. Our results confirm that it is a new, undescribed species, which is confirmed by our morphological observations of floral features. A complete taxonomic treatment of the new species is provided, including a key for the species of the genus in the Atlantic Forest. [ABSTRACT FROM AUTHOR]

Study of recently collected larvae of two species of Ischyomius Chevrolat, 1871 from Napo and Pichincha, Ecuador, tentatively identified as I. denticollis Champion, 1916 and I. singularis Chevrolat, 1871 confirm the classification of the genus Ischyomius within the family Pythidae. Salient larval characters which solidify this placement are: 5 stemmata arranged into an anterior column of 3 and posterior column of 2, parabasal ridges along anterior margins of abdominal tergites, prominent urogomphi with inner urogomphal teeth and various dorsal tubercles, and transverse row of dentiform asperities along anterior margin of sternite 9. Important features of the larva are illustrated with photographs. A key to larvae of world genera of Pythidae is provided as well as natural history information on Ischyomius. [ABSTRACT FROM AUTHOR]

Recent cladistic analysis Of Multiple molecular data from chloroplast and nuclear genomes as well as morphological data have indicated that a reclassification of the family Costaceae is necessary in order to appropriately reflect phylogenetic relationships. The previously described genera Tapeinochilos, Monocostus, and Dimerocostus are all upheld in the new classification. Monocostus and Dimerocostus are found to be sister taxa. The large pantropical genus Costus is found to be polyphyletic and is thus divided into four genera, three of which are new (Cheilocostus, Chamaecostus, Paracostus). Costus has now a more concise generic concept including morphological synapomorphies previously absent due to the polymorphic nature of the prior nonmonophyletic assemblage. Of the three new genera, one (Parocostus) was previously recognized as a subgenus of Costus. Cheilocostus comprises several Asian taxa and is sister to Tapeinochilos, whereas Chamaecostus comprises entirely neotropical taxa and is sister to a neotropical Monocostus + Dimerocostus clade. A reevaluation of the traditional taxonomy indicates that floral characters and pollination syndromes commonly used to identify groups exhibit homoplasy when analyzed in a cladistic framework and are thus unreliable as taxonomic indicators.

This study evaluated the treatment efficiency of municipal wastewater from Phetchaburi in Thailand in an alternated 5-day flooding and 2-day drying wetland system with two plants species, namely, Canna indica and Heliconia psittacorum. The efficiencies of biochemical oxygen demand (BOD5) treatment were in the ranges of 90.5% ± 4.8% and 86.9% ± 7.3% for Canna and Heliconia, respectively. Those of chemical oxygen demand (COD) treatment were in the ranges of 75.5% ± 7.9% and 75.3% ± 9.0% for Canna and Heliconia, respectively. Both plants' removal efficiencies of TN, NH4-N, and TP were greater than 40%. Lead and cadmium accumulation in both plants significantly differed between the upper and lower parts of the plants. However, the lead and cadmium accumulation in Heliconia were greater than their accumulation in Canna. Although Canna had a higher nutrient removal efficiency than Heliconia, there are many varieties of Canna in Thailand. These results indicate that the variety of Canna does not affect the nutrient removal efficiency. In conclusion, a wetland system with alternated flooding and drying conditions can be applied in communities where BOD 5 and COD are the dominant wastewater pollution characteristics. Both ornamental plants are suitable absorbents for lead and cadmium, and although the accumulation is lower in Canna than in Heliconia for both heavy metals, the difference was not significant., (© 2023. The Author(s).)