58 results on '"Zillinger T"'
Search Results
2. 182 Chronic ER stress promotes cGAS/mtDNA-induced autoimmunity via ATF6 in myotonic dystrophy type 2
- Author
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Rösing, S., primary, Ullrich, F., additional, Meisterfeld, S., additional, Schmidt, F., additional, Mlitzko, L., additional, Croon, M., additional, Eberl, N., additional, Schlee, M., additional, Hilbig, D., additional, Reuner, U., additional, Rapp, A., additional, Mirtschink, P., additional, Drukewitz, S., additional, Zillinger, T., additional, Beissert, S., additional, Paeschke, K., additional, Hartmann, G., additional, Trifunovic, A., additional, Bartok, E., additional, and Günther, C., additional
- Published
- 2023
- Full Text
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3. NLRP3-independent inflammasome activation by proteasome inhibitors
- Author
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Ullrich, F., Andryka, K., Schmitz, S., Soler, S. B., Zillinger, T., Hartmann, G., and Bartok, E.
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Medizin ,ComputingMethodologies_GENERAL - Abstract
Poster-Abstract
- Published
- 2021
4. Influence of RNA secondary structure on the activation of Toll-like receptors 7 and 8: W02.002
- Author
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Zillinger, T., Wimmenauer, V., Latz, E., Hartmann, G., and Barchet, W.
- Published
- 2012
5. Building Blocks for a Smart Space for Learning^TM.
- Author
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Simon, B., Sobernig, S., Wild, F., Aguirre, S., Brantner, S., Dolog, P., Neumann, G., Huber, G., Klobucar, T., Markus, S., Miklos, Z., Nejdl, W., Olmedilla, D., Salvachua, J., Sintek, M., and Zillinger, T.
- Published
- 2006
- Full Text
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6. Influence of Rna Secondary Structure on the Activation of Toll-Like Receptors 7 and 8
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Zillinger, T., Wimmenauer, V., Latz, E., Gunther Hartmann, and Barchet, W.
7. Building blocks for a smart space for learning™
- Author
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Simon, B., Sobernig, S., Wild, F., Aguirre, S., Brantner, S., Peter Dolog, Neumann, G., Huber, G., Klobucar, T., Markus, S., Miklos, Z., Nejdl, W., Olmedilla, D., Salvachua, J., Sintek, M., and Zillinger, T.
8. Induction and antiviral activity of ferret myxovirus resistance (Mx) protein 1 against influenza A viruses.
- Author
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Farrukee R, Schwab LSU, Barnes JB, Brooks AG, Londrigan SL, Hartmann G, Zillinger T, and Reading PC
- Subjects
- Animals, Humans, Virus Replication drug effects, Antiviral Agents pharmacology, Cell Line, Mice, Immunity, Innate, Lung virology, Lung immunology, Myxovirus Resistance Proteins genetics, Myxovirus Resistance Proteins metabolism, Ferrets, Influenza A virus immunology, Orthomyxoviridae Infections immunology, Orthomyxoviridae Infections virology
- Abstract
Myxovirus resistance (Mx) proteins are products of interferon stimulated genes (ISGs) and Mx proteins of different species have been reported to mediate antiviral activity against a number of viruses, including influenza A viruses (IAV). Ferrets are widely considered to represent the 'gold standard' small animal model for studying pathogenesis and immunity to human IAV infections, however little is known regarding the antiviral activity of ferret Mx proteins. Herein, we report induction of ferret (f)Mx1/2 in a ferret lung cell line and in airway tissues from IAV-infected ferrets, noting that fMx1 was induced to higher levels that fMx2 both in vitro and in vivo. Overexpression confirmed cytoplasmic expression of fMx1 as well as its ability to inhibit infection and replication of IAV, noting that this antiviral effect of fMx1was modest when compared to cells overexpressing either human MxA or mouse Mx1. Together, these studies provide the first insights regarding the role of fMx1 in cell innate antiviral immunity to influenza viruses. Understanding similarities and differences in the antiviral activities of human and ferret ISGs provides critical context for evaluating results when studying human IAV infections in the ferret model., (© 2024. The Author(s).)
- Published
- 2024
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9. Varicella zoster virus-induced autophagy in human neuronal and hematopoietic cells exerts antiviral activity.
- Author
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Heinz JL, Hinke DM, Maimaitili M, Wang J, Sabli IKD, Thomsen M, Farahani E, Ren F, Hu L, Zillinger T, Grahn A, von Hofsten J, Verjans GMGM, Paludan SR, Viejo-Borbolla A, Sancho-Shimizu V, and Mogensen TH
- Subjects
- Humans, Autophagy-Related Protein-1 Homolog metabolism, Autophagy-Related Protein-1 Homolog genetics, Virus Replication, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Varicella Zoster Virus Infection virology, Viral Envelope Proteins genetics, Viral Envelope Proteins metabolism, Cell Line, Intracellular Signaling Peptides and Proteins metabolism, Intracellular Signaling Peptides and Proteins genetics, Host-Pathogen Interactions, Autophagy, Herpesvirus 3, Human physiology, Herpesvirus 3, Human pathogenicity, Neurons virology
- Abstract
Autophagy is a degradational pathway with pivotal roles in cellular homeostasis and survival, including protection of neurons in the central nervous system (CNS). The significance of autophagy as antiviral defense mechanism is recognized and some viruses hijack and modulate this process to their advantage in certain cell types. Here, we present data demonstrating that the human neurotropic herpesvirus varicella zoster virus (VZV) induces autophagy in human SH-SY5Y neuronal cells, in which the pathway exerts antiviral activity. Productively VZV-infected SH-SY5Y cells showed increased LC3-I-LC3-II conversion as well as co-localization of the viral glycoprotein E and the autophagy receptor p62. The activation of autophagy was dependent on a functional viral genome. Interestingly, inducers of autophagy reduced viral transcription, whereas inhibition of autophagy increased viral transcript expression. Finally, the genotype of patients with severe ocular and brain VZV infection were analyzed to identify potential autophagy-associated inborn errors of immunity. Two patients expressing genetic variants in the autophagy genes ULK1 and MAP1LC3B2, respectively, were identified. Notably, cells of both patients showed reduced autophagy, alongside enhanced viral replication and death of VZV-infected cells. In conclusion, these results demonstrate a neuro-protective role for autophagy in the context of VZV infection and suggest that failure to mount an autophagy response is a potential predisposing factor for development of severe VZV disease., (© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.)
- Published
- 2024
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10. Immune Profiling of Vulvar Squamous Cell Cancer Discovers a Macrophage-rich Subtype Associated with Poor Prognosis.
- Author
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Condic M, Rohr A, Riemann S, Staerk C, Ayub TH, Doeser A, Zillinger T, Merkelbach-Bruse S, Buettner R, Barchet W, Rudlowski C, Mustea A, and Kübler K
- Subjects
- Female, Humans, Biomarkers, Tumor analysis, Vascular Endothelial Growth Factor A, Prognosis, Epithelial Cells chemistry, Vulvar Neoplasms metabolism, Carcinoma, Squamous Cell metabolism
- Abstract
The incidence rates of vulvar squamous cell cancer (VSCC) have increased over the past decades, requiring personalized oncologic approaches. Currently, lymph node involvement is a key factor in determining prognosis and treatment options. However, there is a need for additional immune-related biomarkers to provide more precise treatment and prognostic information. Here, we used IHC and expression data to characterize immune cells and their spatial distribution in VSCC. Hierarchical clustering analysis identified distinct immune subtypes, of which the macrophage-rich subtype was associated with adverse outcome. This is consistent with our findings of increased lymphogenesis, lymphatic invasion, and lymph node involvement associated with high macrophage infiltration. Further in vitro studies showed that VSCC-associated macrophages expressed VEGF-A and subsequently induced VEGF-A in the VSCC cell line A-431, providing experimental support for a pro-lymphangiogenic role of macrophages in VSCC. Taken together, immune profiling in VSCC revealed tumor processes, identified a subset of patients with adverse outcome, and provided a valuable biomarker for risk stratification and therapeutic decision making for anti-VEGF treatment, ultimately contributing to the advancement of precision medicine in VSCC., Significance: Immunoprofiling in VSCC reveals subtypes with distinct clinical and biological behavior. Of these, the macrophage-rich VSCC subtype is characterized by poor clinical outcome and increased VEGF-A expression, providing a biomarker for risk stratification and therapeutic sensitivity., (© 2024 The Authors; Published by the American Association for Cancer Research.)
- Published
- 2024
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11. Chronic endoplasmic reticulum stress in myotonic dystrophy type 2 promotes autoimmunity via mitochondrial DNA release.
- Author
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Rösing S, Ullrich F, Meisterfeld S, Schmidt F, Mlitzko L, Croon M, Nattrass RG, Eberl N, Mahlberg J, Schlee M, Wieland A, Simon P, Hilbig D, Reuner U, Rapp A, Bremser J, Mirtschink P, Drukewitz S, Zillinger T, Beissert S, Paeschke K, Hartmann G, Trifunovic A, Bartok E, and Günther C
- Subjects
- Humans, DNA, Mitochondrial genetics, Autoimmunity genetics, Leukocytes, Mononuclear metabolism, RNA, Nucleotidyltransferases genetics, Nucleotidyltransferases metabolism, Endoplasmic Reticulum Stress genetics, Myotonic Dystrophy genetics, Myotonic Dystrophy metabolism, Interferon Type I, Autoimmune Diseases
- Abstract
Myotonic dystrophy type 2 (DM2) is a tetranucleotide CCTG repeat expansion disease associated with an increased prevalence of autoimmunity. Here, we identified an elevated type I interferon (IFN) signature in peripheral blood mononuclear cells and primary fibroblasts of DM2 patients as a trigger of chronic immune stimulation. Although RNA-repeat accumulation was prevalent in the cytosol of DM2-patient fibroblasts, type-I IFN release did not depend on innate RNA immune sensors but rather the DNA sensor cGAS and the prevalence of mitochondrial DNA (mtDNA) in the cytoplasm. Sublethal mtDNA release was promoted by a chronic activation of the ATF6 branch of the unfolded protein response (UPR) in reaction to RNA-repeat accumulation and non-AUG translated tetrapeptide expansion proteins. ATF6-dependent mtDNA release and resulting cGAS/STING activation could also be recapitulated in human THP-1 monocytes exposed to chronic endoplasmic reticulum (ER) stress. Altogether, our study demonstrates a novel mechanism by which large repeat expansions cause chronic endoplasmic reticulum stress and associated mtDNA leakage. This mtDNA is, in turn, sensed by the cGAS/STING pathway and induces a type-I IFN response predisposing to autoimmunity. Elucidating this pathway reveals new potential therapeutic targets for autoimmune disorders associated with repeat expansion diseases., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
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12. A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNA.
- Author
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de Regt AK, Anand K, Ciupka K, Bender F, Gatterdam K, Putschli B, Fusshöller D, Hilbig D, Kirchhoff A, Hunkler C, Wolter S, Grünewald A, Wallerath C, Schuberth-Wagner C, Ludwig J, Paeschke K, Bartok E, Hagelueken G, Hartmann G, Zillinger T, Geyer M, and Schlee M
- Subjects
- Immune Tolerance, RNA, Double-Stranded genetics, RNA, Mitochondrial genetics, RNA, Mitochondrial metabolism, RNA, Viral genetics, RNA, Viral metabolism, Humans, DEAD Box Protein 58 chemistry, DEAD Box Protein 58 genetics, DEAD Box Protein 58 metabolism, Isoleucine genetics, Receptors, Immunologic chemistry, Receptors, Immunologic genetics, Receptors, Immunologic metabolism
- Abstract
RIG-I is a cytosolic receptor of viral RNA essential for the immune response to numerous RNA viruses. Accordingly, RIG-I must sensitively detect viral RNA yet tolerate abundant self-RNA species. The basic binding cleft and an aromatic amino acid of the RIG-I C-terminal domain(CTD) mediate high-affinity recognition of 5'triphosphorylated and 5'base-paired RNA(dsRNA). Here, we found that, while 5'unmodified hydroxyl(OH)-dsRNA demonstrated residual activation potential, 5'-monophosphate(5'p)-termini, present on most cellular RNAs, prevented RIG-I activation. Determination of CTD/dsRNA co-crystal structures and mutant activation studies revealed that the evolutionarily conserved I875 within the CTD sterically inhibits 5'p-dsRNA binding. RIG-I(I875A) was activated by both synthetic 5'p-dsRNA and endogenous long dsRNA within the polyA-rich fraction of total cellular RNA. RIG-I(I875A) specifically interacted with long, polyA-bearing, mitochondrial(mt) RNA, and depletion of mtRNA from total RNA abolished its activation. Altogether, our study demonstrates that avoidance of 5'p-RNA recognition is crucial to prevent mtRNA-triggered RIG-I-mediated autoinflammation., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2023
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13. C-terminal variants in CDC42 drive type I interferon-dependent autoinflammation in NOCARH syndrome reversible by ruxolitinib.
- Author
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Kapp FG, Kretschmer S, Beckmann CCA, Wäsch L, Molitor A, Carapito R, Schubert M, Lucas N, Conrad S, Poignant S, Isidor B, Rohlfs M, Kisaarslan AP, Schanze D, Zenker M, Schmitt-Graeff A, Strahm B, Peters A, Yoshimi A, Driever W, Zillinger T, Günther C, Maharana S, Guan K, Klein C, Ehl S, Niemeyer CM, Unal E, Bahram S, Hauck F, Lee-Kirsch MA, and Speckmann C
- Subjects
- Humans, Infant, Newborn, cdc42 GTP-Binding Protein, Inflammasomes genetics, Nitriles, Syndrome, Interferon Type I, Lymphohistiocytosis, Hemophagocytic etiology
- Abstract
C-terminal variants in CDC42 encoding cell division control protein 42 homolog underlie neonatal-onset cytopenia, autoinflammation, rash, and hemophagocytic lymphohistiocytosis (NOCARH). Pyrin inflammasome hyperactivation has been shown to contribute to disease pathophysiology. However, mortality of NOCARH patients remains high despite inflammasome-focused treatments. Here, we demonstrate in four NOCARH patients from three families that cell-intrinsic activation of type I interferon (IFN) is a previously unrecognized driver of autoinflammation in NOCARH. Our data show that aberrant innate immune activation is caused by sensing of cytosolic nucleic acids released from mitochondria, which exhibit disturbances in integrity and dynamics due to CDC42 dysfunction. In one of our patients, treatment with the Janus kinase inhibitor ruxolitinib led to complete remission, indicating that inhibition of type I IFN signaling may have an important role in the management of autoinflammation in patients with NOCARH., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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14. Inhibition of cellular RNA methyltransferase abrogates influenza virus capping and replication.
- Author
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Tsukamoto Y, Hiono T, Yamada S, Matsuno K, Faist A, Claff T, Hou J, Namasivayam V, Vom Hemdt A, Sugimoto S, Ng JY, Christensen MH, Tesfamariam YM, Wolter S, Juranek S, Zillinger T, Bauer S, Hirokawa T, Schmidt FI, Kochs G, Shimojima M, Huang YS, Pichlmair A, Kümmerer BM, Sakoda Y, Schlee M, Brunotte L, Müller CE, Igarashi M, and Kato H
- Subjects
- Animals, Humans, Mice, RNA, Messenger metabolism, RNA, Viral biosynthesis, Streptomyces chemistry, Computer Simulation, A549 Cells, RNA Caps metabolism, Virus Replication drug effects, Alphainfluenzavirus drug effects, Betainfluenzavirus drug effects, Biological Products chemistry, Biological Products pharmacology, Antiviral Agents chemistry, Antiviral Agents pharmacology, Tubercidin analogs & derivatives, Tubercidin pharmacology, Methyltransferases antagonists & inhibitors, Enzyme Inhibitors chemistry, Enzyme Inhibitors pharmacology
- Abstract
Orthomyxo- and bunyaviruses steal the 5' cap portion of host RNAs to prime their own transcription in a process called "cap snatching." We report that RNA modification of the cap portion by host 2'-O-ribose methyltransferase 1 (MTr1) is essential for the initiation of influenza A and B virus replication, but not for other cap-snatching viruses. We identified with in silico compound screening and functional analysis a derivative of a natural product from Streptomyces , called trifluoromethyl-tubercidin (TFMT), that inhibits MTr1 through interaction at its S -adenosyl-l-methionine binding pocket to restrict influenza virus replication. Mechanistically, TFMT impairs the association of host cap RNAs with the viral polymerase basic protein 2 subunit in human lung explants and in vivo in mice. TFMT acts synergistically with approved anti-influenza drugs.
- Published
- 2023
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15. Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver T RM cells.
- Author
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Valencia-Hernandez AM, Zillinger T, Ge Z, Tan PS, Cozijnsen A, I McFadden G, Lahoud MH, Caminschi I, Barchet W, Heath WR, and Fernandez-Ruiz D
- Subjects
- Humans, Toll-Like Receptor 9, Adjuvants, Immunologic pharmacology, Oligodeoxyribonucleotides pharmacology, CD8-Positive T-Lymphocytes, Liver, Liposomes, Vaccines
- Abstract
Tissue resident memory T cells (T
RM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating TRM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8+ TRM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8+ TRM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remain the most efficient inducers of liver TRM cells among all adjuvants tested. Moreover, their combination with the cationic liposome DOTAP further enhances the potency, particularly of the class B ODN CpG 1668 and the human TLR9 ligand CpG 2006 (CpG 7909). This study informs the design of efficient liver TRM -based vaccines for their potential translation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2023
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16. Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner.
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Schumann T, Ramon SC, Schubert N, Mayo MA, Hega M, Maser KI, Ada SR, Sydow L, Hajikazemi M, Badstübner M, Müller P, Ge Y, Shakeri F, Buness A, Rupf B, Lienenklaus S, Utess B, Muhandes L, Haase M, Rupp L, Schmitz M, Gramberg T, Manel N, Hartmann G, Zillinger T, Kato H, Bauer S, Gerbaulet A, Paeschke K, Roers A, and Behrendt R
- Subjects
- Mice, Animals, SAM Domain and HD Domain-Containing Protein 1 genetics, Immunity, Innate genetics, Membrane Proteins metabolism, Nucleotidyltransferases metabolism, Interferon Type I metabolism, Nucleic Acids
- Abstract
Defects in nucleic acid metabolizing enzymes can lead to spontaneous but selective activation of either cGAS/STING or RIG-like receptor (RLR) signaling, causing type I interferon-driven inflammatory diseases. In these pathophysiological conditions, activation of the DNA sensor cGAS and IFN production are linked to spontaneous DNA damage. Physiological, or tonic, IFN signaling on the other hand is essential to functionally prime nucleic acid sensing pathways. Here, we show that low-level chronic DNA damage in mice lacking the Aicardi-Goutières syndrome gene SAMHD1 reduced tumor-free survival when crossed to a p53-deficient, but not to a DNA mismatch repair-deficient background. Increased DNA damage did not result in higher levels of type I interferon. Instead, we found that the chronic interferon response in SAMHD1-deficient mice was driven by the MDA5/MAVS pathway but required functional priming through the cGAS/STING pathway. Our work positions cGAS/STING upstream of tonic IFN signaling in Samhd1-deficient mice and highlights an important role of the pathway in physiological and pathophysiological innate immune priming., (© 2022 Schumann et al.)
- Published
- 2023
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17. Retinoic Acid-Inducible Gene I Activation Inhibits Human Respiratory Syncytial Virus Replication in Mammalian Cells and in Mouse and Ferret Models of Infection.
- Author
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Schwab LSU, Farrukee R, Eléouët JF, Rameix-Welti MA, Londrigan SL, Brooks AG, Hurt AC, Coch C, Zillinger T, Hartmann G, and Reading PC
- Subjects
- Animals, Humans, Ferrets, Lung, Virus Replication, Antiviral Agents pharmacology, Tretinoin, Respiratory Syncytial Virus, Human physiology, Respiratory Syncytial Virus Infections
- Abstract
Infections caused by human respiratory syncytial virus (RSV) are associated with substantial rates of morbidity and mortality. Treatment options are limited, and there is urgent need for the development of efficient antivirals. Pattern recognition receptors such as the cytoplasmic helicase retinoic acid-inducible gene (RIG) I can be activated by viral nucleic acids, leading to activation of interferon-stimulated genes and generation of an "antiviral state." In the current study, we activated RIG-I with synthetic RNA agonists (3pRNA) to induce resistance to RSV infection in vitro and in vivo. In vitro, pretreatment of human, mouse, and ferret airway cell lines with RIG-I agonist before RSV exposure inhibited virus infection and replication. Moreover, a single intravenous injection of 3pRNA 1 day before RSV infection resulted in potent inhibition of virus replication in the lungs of mice and ferrets, but not in nasal tissues. These studies provide evidence that RIG-I agonists represent a promising antiviral drug for RSV prophylaxis., Competing Interests: Potential conflicts of interest. G. H. is an inventor on a patent about RIG-I ligands. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed., (© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)
- Published
- 2022
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18. Correction: Monocyte-derived macrophages aggravate pulmonary vasculitis via cGAS/STING/IFN-mediated nucleic acid sensing.
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Kessler N, Viehmann SF, Krollmann C, Mai K, Kirschner KM, Luksch H, Kotagiri P, Böhner AMC, Huugen D, de Oliveira Mann CC, Otten S, Weiss SAI, Zillinger T, Dobrikova K, Jenne DE, Behrendt R, Ablasser A, Bartok E, Hartmann G, Hopfner KP, Lyons PA, Boor P, Rösen-Wolff A, Teichmann LL, Heeringa P, Kurts C, and Garbi N
- Published
- 2022
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19. SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA.
- Author
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Maharana S, Kretschmer S, Hunger S, Yan X, Kuster D, Traikov S, Zillinger T, Gentzel M, Elangovan S, Dasgupta P, Chappidi N, Lucas N, Maser KI, Maatz H, Rapp A, Marchand V, Chang YT, Motorin Y, Hubner N, Hartmann G, Hyman AA, Alberti S, and Lee-Kirsch MA
- Subjects
- Antiviral Agents, Autoimmune Diseases of the Nervous System, Exonucleases genetics, Humans, Immunity, Innate genetics, Nervous System Malformations, SAM Domain and HD Domain-Containing Protein 1 genetics, Interferon Type I genetics, RNA, Double-Stranded genetics
- Abstract
Recognition of pathogen-derived foreign nucleic acids is central to innate immune defense. This requires discrimination between structurally highly similar self and nonself nucleic acids to avoid aberrant inflammatory responses as in the autoinflammatory disorder Aicardi-Goutières syndrome (AGS). How vast amounts of self RNA are shielded from immune recognition to prevent autoinflammation is not fully understood. Here, we show that human SAM-domain- and HD-domain-containing protein 1 (SAMHD1), one of the AGS-causing genes, functions as a single-stranded RNA (ssRNA) 3'exonuclease, the lack of which causes cellular RNA accumulation. Increased ssRNA in cells leads to dissolution of RNA-protein condensates, which sequester immunogenic double-stranded RNA (dsRNA). Release of sequestered dsRNA from condensates triggers activation of antiviral type I interferon via retinoic-acid-inducible gene I-like receptors. Our results establish SAMHD1 as a key regulator of cellular RNA homeostasis and demonstrate that buffering of immunogenic self RNA by condensates regulates innate immune responses., Competing Interests: Declaration of interests A.A.H. is a founder of Dewpoint Therapeutics and Caraway Therapeutics and is an advisor to Dewpoint therapeutics. S.A. is an advisor to Dewpoint Therapeutics., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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20. Monocyte-derived macrophages aggravate pulmonary vasculitis via cGAS/STING/IFN-mediated nucleic acid sensing.
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Kessler N, Viehmann SF, Krollmann C, Mai K, Kirschner KM, Luksch H, Kotagiri P, Böhner AMC, Huugen D, de Oliveira Mann CC, Otten S, Weiss SAI, Zillinger T, Dobrikova K, Jenne DE, Behrendt R, Ablasser A, Bartok E, Hartmann G, Hopfner KP, Lyons PA, Boor P, Rösen-Wolff A, Teichmann LL, Heeringa P, Kurts C, and Garbi N
- Subjects
- Animals, Lung, Macrophages, Membrane Proteins metabolism, Mice, Nucleotidyltransferases, Interferon Type I, Nucleic Acids, Vasculitis
- Abstract
Autoimmune vasculitis is a group of life-threatening diseases, whose underlying pathogenic mechanisms are incompletely understood, hampering development of targeted therapies. Here, we demonstrate that patients suffering from anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) showed increased levels of cGAMP and enhanced IFN-I signature. To identify disease mechanisms and potential therapeutic targets, we developed a mouse model for pulmonary AAV that mimics severe disease in patients. Immunogenic DNA accumulated during disease onset, triggering cGAS/STING/IRF3-dependent IFN-I release that promoted endothelial damage, pulmonary hemorrhages, and lung dysfunction. Macrophage subsets played dichotomic roles in disease. While recruited monocyte-derived macrophages were major disease drivers by producing most IFN-β, resident alveolar macrophages contributed to tissue homeostasis by clearing red blood cells and limiting infiltration of IFN-β-producing macrophages. Moreover, pharmacological inhibition of STING, IFNAR-I, or its downstream JAK/STAT signaling reduced disease severity and accelerated recovery. Our study unveils the importance of STING/IFN-I axis in promoting pulmonary AAV progression and identifies cellular and molecular targets to ameliorate disease outcomes., (© 2022 Kessler et al.)
- Published
- 2022
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21. Induction of Interferon-Stimulated Genes Correlates with Reduced Growth of Influenza A Virus in Lungs after RIG-I Agonist Treatment of Ferrets.
- Author
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Schwab LSU, Londrigan SL, Brooks AG, Hurt AC, Sahu A, Deng YM, Moselen J, Coch C, Zillinger T, Hartmann G, and Reading PC
- Subjects
- Animals, Antiviral Agents pharmacology, Ferrets metabolism, Humans, Immunity, Innate, Interferons metabolism, Leukocytes, Mononuclear metabolism, Lung, Mice, Virus Replication genetics, Influenza A virus genetics, Influenza, Human
- Abstract
Intracellular RIG-I receptors represent key innate sensors of RNA virus infection, and RIG-I activation results in the induction of hundreds of host effector genes, including interferon-stimulated genes (ISGs). Synthetic RNA agonists targeting RIG-I have shown promise as antivirals against a broad spectrum of viruses, including influenza A virus (IAV), in both in vitro and mouse models of infection. Herein, we demonstrate that treatment of a ferret airway epithelial (FRL) cell line with a RIG-I agonist rapidly and potently induced expression of a broad range of ISGs and resulted in potent inhibition of growth of different IAV strains. In ferrets, a single intravenous injection of RIG-I agonist was associated with upregulated ISG expression in peripheral blood mononuclear cells and lung tissue, but not in nasal tissues. In a ferret model of viral contact transmission, a single treatment of recipient animals 24 h prior to cohousing with IAV-infected donors did not reduce virus transmission and shedding but did result in reduced lung virus titers 6 days after treatment. A single treatment of the IAV-infected donor animals also resulted in reduced virus titers in the lungs 2 days later. Thus, a single intravenous treatment with RIG-I agonist prior to infection or to ferrets with an established IAV infection can reduce virus growth in the lungs. These findings support further development of RIG-I agonists as effective antiviral treatments to limit the impact of IAV infections, particularly in reducing virus replication in the lower airways. IMPORTANCE RIG-I agonists have shown potential as broad-spectrum antivirals in vitro and in mouse models of infection. However, their antiviral potential has not been reported in outbred animals such as ferrets, which are widely regarded as the gold standard small animal model for human IAV infections. Herein, we demonstrate that RIG-I agonist treatment of a ferret airway cell line resulted in ISG induction and inhibition of a broad range of human influenza viruses. A single intravenous treatment of ferrets also resulted in systemic induction of ISGs, including in lung tissue, and when delivered to animals prior to IAV exposure or to animals with established IAV infection treatment resulted in reduced virus replication in the lungs. These data demonstrate the effectiveness of single RIG-I treatment against IAV in the ferret model and highlight the importance of future studies to optimize treatment regimens and delivery routes to maximize their ability to ameliorate IAV infections.
- Published
- 2022
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22. Expression of a Functional Mx1 Protein Is Essential for the Ability of RIG-I Agonist Prophylaxis to Provide Potent and Long-Lasting Protection in a Mouse Model of Influenza A Virus Infection.
- Author
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Schwab LSU, Villalón-Letelier F, Tessema MB, Londrigan SL, Brooks AG, Hurt A, Coch C, Zillinger T, Hartmann G, and Reading PC
- Subjects
- Animals, Antiviral Agents, Influenza A virus, Interferons, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Proteins, DEAD Box Protein 58, Myxovirus Resistance Proteins genetics, Orthomyxoviridae Infections
- Abstract
RIG-I is an innate sensor of RNA virus infection and its activation induces interferon-stimulated genes (ISGs). In vitro studies using human cells have demonstrated the ability of synthetic RIG-I agonists (3pRNA) to inhibit IAV replication. However, in mouse models of IAV the effectiveness of 3pRNA reported to date differs markedly between studies. Myxoma resistance (Mx)1 is an ISG protein which mediates potent anti-IAV activity, however most inbred mouse strains do not express a functional Mx1. Herein, we utilised C57BL/6 mice that do (B6.A2G- Mx1 ) and do not (B6-WT) express functional Mx1 to assess the ability of prophylactic 3pRNA treatment to induce ISGs and to protect against subsequent IAV infection. In vitro, 3pRNA treatment of primary lung cells from B6-WT and B6.A2G- Mx1 mice resulted in ISG induction however inhibition of IAV infection was more potent in cells from B6.A2G- Mx1 mice. In vivo, a single intravenous injection of 3pRNA resulted in ISG induction in lungs of both B6-WT and B6.A2G- Mx1 mice, however potent and long-lasting protection against subsequent IAV challenge was only observed in B6.A2G- Mx1 mice. Thus, despite broad ISG induction, expression of a functional Mx1 is critical for potent and long-lasting RIG-I agonist-mediated protection in the mouse model of IAV infection.
- Published
- 2022
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23. Deficiency in coatomer complex I causes aberrant activation of STING signalling.
- Author
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Steiner A, Hrovat-Schaale K, Prigione I, Yu CH, Laohamonthonkul P, Harapas CR, Low RRJ, De Nardo D, Dagley LF, Mlodzianoski MJ, Rogers KL, Zillinger T, Hartmann G, Gantier MP, Gattorno M, Geyer M, Volpi S, Davidson S, and Masters SL
- Subjects
- COP-Coated Vesicles metabolism, Coat Protein Complex I metabolism, Electron Transport Complex I metabolism, Humans, Signal Transduction, Leukocytes, Mononuclear metabolism, Nucleotidyltransferases genetics, Nucleotidyltransferases metabolism
- Abstract
Coatomer complex I (COPI) mediates retrograde vesicular trafficking from Golgi to the endoplasmic reticulum (ER) and within Golgi compartments. Deficiency in subunit alpha causes COPA syndrome and is associated with type I IFN signalling, although the upstream innate immune sensor involved was unknown. Using in vitro models we find aberrant activation of the STING pathway due to deficient retrograde but probably not intra-Golgi transport. Further we find the upstream cytosolic DNA sensor cGAS as essentially required to drive type I IFN signalling. Genetic deletion of COPI subunits COPG1 or COPD similarly induces type I IFN activation in vitro, which suggests that inflammatory diseases associated with mutations in other COPI subunit genes may exist. Finally, we demonstrate that inflammation in COPA syndrome patient peripheral blood mononuclear cells and COPI-deficient cell lines is ameliorated by treatment with the small molecule STING inhibitor H-151, suggesting targeted inhibition of the cGAS/STING pathway as a promising therapeutic approach., (© 2022. The Author(s).)
- Published
- 2022
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24. Recessive NLRC4-Autoinflammatory Disease Reveals an Ulcerative Colitis Locus.
- Author
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Steiner A, Reygaerts T, Pontillo A, Ceccherini I, Moecking J, Moghaddas F, Davidson S, Caroli F, Grossi A, Castro FFM, Kalil J, Gohr FN, Schmidt FI, Bartok E, Zillinger T, Hartmann G, Geyer M, Gattorno M, Mendonça LO, and Masters SL
- Subjects
- CARD Signaling Adaptor Proteins genetics, CARD Signaling Adaptor Proteins metabolism, Calcium-Binding Proteins genetics, Female, Genome-Wide Association Study, Humans, Inflammasomes metabolism, Middle Aged, Colitis, Ulcerative diagnosis, Colitis, Ulcerative genetics, Hereditary Autoinflammatory Diseases
- Abstract
Purpose: NLRC4-associated autoinflammatory disease (NLRC4-AID) is an autosomal dominant condition presenting with a range of clinical manifestations which can include macrophage activation syndrome (MAS) and severe enterocolitis. We now report the first homozygous mutation in NLRC4 (c.478G > A, p.A160T) causing autoinflammatory disease with immune dysregulation and find that heterozygous carriers in the general population are at increased risk of developing ulcerative colitis., Methods: Circulating immune cells and inflammatory markers were profiled and historical clinical data interrogated. DNA was extracted and sequenced using standard procedures. Inflammasome activation assays for ASC speck formation, pyroptosis, and IL-1β/IL-18 secretion confirmed pathogenicity of the mutation in vitro. Genome-wide association of NLRC4 (A160T) with ulcerative colitis was examined using data from the IBD exomes portal., Results: A 60-year-old Brazilian female patient was evaluated for recurrent episodes of systemic inflammation from six months of age. Episodes were characterized by recurrent low-grade fever, chills, oral ulceration, uveitis, arthralgia, and abdominal pain, followed by diarrhea with mucus and variable skin rash. High doses of corticosteroids were somewhat effective in controlling disease and anti-IL-1β therapy partially controlled symptoms. While on treatment, serum IL-1β and IL-18 levels remained elevated. Genetic investigations identified a homozygous mutation in NLRC4 (A160T), inherited in a recessive fashion. Increased ASC speck formation and IL-1β/IL-18 secretion confirmed pathogenicity when NLRC4 (A160T) was analyzed in human cell lines. This allele is significantly enriched in patients with ulcerative colitis: OR 2.546 (95% 1.778-3.644), P = 0.01305., Conclusion: NLRC4 (A160T) can either cause recessively inherited autoinflammation and immune dysregulation, or function as a heterozygous risk factor for the development of ulcerative colitis., (© 2021. The Author(s).)
- Published
- 2022
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25. Animal models of SARS-CoV-2 and COVID-19 for the development of prophylactic and therapeutic interventions.
- Author
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Renn M, Bartok E, Zillinger T, Hartmann G, and Behrendt R
- Subjects
- Animals, Disease Models, Animal, Drug Development, COVID-19 Drug Treatment
- Abstract
Infections of the Coronavirus SARS-CoV-2 continue to spread around the globe, causing Coronavirus Disease (COVID)-19. Infected people are at risk of developing acute interstitial pneumonia, which can result in lethal complications, particularly in patients with pre-existing co-morbidities. Novel prophylactic and therapeutic interventions are urgently needed to limit the infection-associated health risk for the population and to contain the pandemic. Animal models are indispensable to assessing the efficacy and safety of potential new antivirals, vaccines, and other innovative therapies, such as nucleic acid agonists of innate immune sensing receptors. In this review, we provide an overview of the commonly used animal models to study SARS-CoV-2 and COVID-19, including a summary of their susceptibility to infection, the spectrum of symptoms elicited, and the potential for drug development in each model. We hope that this review will help researchers to decide on the right model organism to quickly address their specific scientific questions., Competing Interests: Declaration of Competing Interest Marcel Renn and Gunther Hartmann were co-founders of Rigontec. All other authors declare that there are no conflicts of interest., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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26. MAPK-pathway inhibition mediates inflammatory reprogramming and sensitizes tumors to targeted activation of innate immunity sensor RIG-I.
- Author
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Brägelmann J, Lorenz C, Borchmann S, Nishii K, Wegner J, Meder L, Ostendorp J, Ast DF, Heimsoeth A, Nakasuka T, Hirabae A, Okawa S, Dammert MA, Plenker D, Klein S, Lohneis P, Gu J, Godfrey LK, Forster J, Trajkovic-Arsic M, Zillinger T, Haarmann M, Quaas A, Lennartz S, Schmiel M, D'Rozario J, Thomas ES, Li H, Schmitt CA, George J, Thomas RK, von Karstedt S, Hartmann G, Büttner R, Ullrich RT, Siveke JT, Ohashi K, Schlee M, and Sos ML
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Animals, Cell Cycle Checkpoints drug effects, Cell Death drug effects, Cell Line, Tumor, Cytokines metabolism, ErbB Receptors metabolism, Female, Gene Expression Regulation, Neoplastic drug effects, Humans, Immune Evasion drug effects, Interferon Regulatory Factor-1 metabolism, Mice, Mice, Inbred C57BL, Neoplasms pathology, Oncogenes, Signal Transduction drug effects, DEAD Box Protein 58 metabolism, Immunity, Innate drug effects, Inflammation pathology, MAP Kinase Signaling System drug effects, Neoplasms metabolism, Protein Kinase Inhibitors pharmacology, Receptors, Immunologic metabolism
- Abstract
Kinase inhibitors suppress the growth of oncogene driven cancer but also enforce the selection of treatment resistant cells that are thought to promote tumor relapse in patients. Here, we report transcriptomic and functional genomics analyses of cells and tumors within their microenvironment across different genotypes that persist during kinase inhibitor treatment. We uncover a conserved, MAPK/IRF1-mediated inflammatory response in tumors that undergo stemness- and senescence-associated reprogramming. In these tumor cells, activation of the innate immunity sensor RIG-I via its agonist IVT4, triggers an interferon and a pro-apoptotic response that synergize with concomitant kinase inhibition. In humanized lung cancer xenografts and a syngeneic Egfr-driven lung cancer model these effects translate into reduction of exhausted CD8
+ T cells and robust tumor shrinkage. Overall, the mechanistic understanding of MAPK/IRF1-mediated intratumoral reprogramming may ultimately prolong the efficacy of targeted drugs in genetically defined cancer patients., (© 2021. The Author(s).)- Published
- 2021
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27. ADAR1 edits the SenZ and SenZ-ability of RNA.
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Zillinger T and Bartok E
- Subjects
- Adenosine Deaminase genetics, Adenosine Deaminase metabolism, Animals, Mice, RNA Editing, RNA-Binding Proteins genetics, RNA-Binding Proteins metabolism, Autoimmune Diseases of the Nervous System, RNA genetics
- Abstract
Some RNAs can assume a Z conformation, an unusual, left-handed turn. In this issue of Immunity, three studies report that mutations in the Zα-RNA binding domain of the adenosine deaminase ADAR1 are sufficient to induce autoinflammatory disease in mice, which models human Aicardí-Goutières syndrome, highlighting the important role of Z-RNA editing in limiting innate immune recognition of endogenous RNA., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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28. Author Correction: An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1.
- Author
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Wegner J, Zillinger T, Schlee-Guimaraes TM, Bartok E, and Schlee M
- Published
- 2021
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29. Malaria parasites both repress host CXCL10 and use it as a cue for growth acceleration.
- Author
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Ofir-Birin Y, Ben Ami Pilo H, Cruz Camacho A, Rudik A, Rivkin A, Revach OY, Nir N, Block Tamin T, Abou Karam P, Kiper E, Peleg Y, Nevo R, Solomon A, Havkin-Solomon T, Rojas A, Rotkopf R, Porat Z, Avni D, Schwartz E, Zillinger T, Hartmann G, Di Pizio A, Quashie NB, Dikstein R, Gerlic M, Torrecilhas AC, Levy C, Nolte-'t Hoen ENM, Bowie AG, and Regev-Rudzki N
- Subjects
- 3' Untranslated Regions, Chemokine CXCL10 genetics, DEAD Box Protein 58 metabolism, ELAV-Like Protein 1 metabolism, Extracellular Vesicles metabolism, Host-Parasite Interactions, Humans, Life Cycle Stages, Malaria, Falciparum immunology, Monocytes metabolism, Plasmodium falciparum growth & development, Plasmodium falciparum metabolism, Protein Biosynthesis, RNA, Protozoan metabolism, Receptors, Immunologic metabolism, Ribosomes metabolism, THP-1 Cells, Chemokine CXCL10 metabolism, Malaria, Falciparum parasitology, Plasmodium falciparum physiology
- Abstract
Pathogens are thought to use host molecular cues to control when to initiate life-cycle transitions, but these signals are mostly unknown, particularly for the parasitic disease malaria caused by Plasmodium falciparum. The chemokine CXCL10 is present at high levels in fatal cases of cerebral malaria patients, but is reduced in patients who survive and do not have complications. Here we show a Pf 'decision-sensing-system' controlled by CXCL10 concentration. High CXCL10 expression prompts P. falciparum to initiate a survival strategy via growth acceleration. Remarkably, P. falciparum inhibits CXCL10 synthesis in monocytes by disrupting the association of host ribosomes with CXCL10 transcripts. The underlying inhibition cascade involves RNA cargo delivery into monocytes that triggers RIG-I, which leads to HUR1 binding to an AU-rich domain of the CXCL10 3'UTR. These data indicate that when the parasite can no longer keep CXCL10 at low levels, it can exploit the chemokine as a cue to shift tactics and escape., (© 2021. The Author(s).)
- Published
- 2021
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30. An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1.
- Author
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Wegner J, Zillinger T, Schlee-Guimaraes TM, Bartok E, and Schlee M
- Subjects
- B-Lymphocytes drug effects, B-Lymphocytes immunology, Cell Transdifferentiation, Epigenesis, Genetic, GPI-Linked Proteins, Humans, Lipopolysaccharides pharmacology, Models, Biological, Primary Cell Culture, Signal Transduction, Single-Cell Analysis, THP-1 Cells, B-Lymphocytes cytology, Membrane Proteins genetics, Toll-Like Receptor 4 metabolism
- Abstract
Antigen-presenting myeloid cells like monocytes detect invading pathogens via pattern recognition receptors (PRRs) and initiate adaptive and innate immune responses. As analysis of PRR signaling in primary human monocytes is hampered by their restricted expandability, human monocyte models like THP-1 cells are commonly used for loss-of-function studies, such as with CRISPR-Cas9 editing. A recently developed transdifferentiation cell culture system, BLaER1, enables lineage conversion from malignant B cells to monocytes and was found superior to THP-1 in mimicking PRR signaling, thus being the first model allowing TLR4 and inflammasome pathway analysis. Here, we identified an important caveat when investigating TLR4-driven signaling in BLaER1 cells. We show that this model contains glycosylphosphatidylinositol (GPI) anchor-deficient cells, which lack CD14 surface expression when differentiated to monocytes, resulting in diminished LPS/TLR4 but not TLR7/TLR8 responsiveness. This GPI anchor defect is caused by epigenetic silencing of PIGH, leading to a random distribution of intact and PIGH-deficient clones after single-cell cloning. Overexpressing PIGH restored GPI-anchored protein (including CD14) expression and LPS responsiveness. When studying CD14- or other GPI-anchored protein-dependent pathways, researchers should consider this anomaly and ensure equal GPI-anchored protein expression when comparing cells that have undergone single-cell cloning, e. g. after CRISPR-Cas9 editing., (© 2021. The Author(s).)
- Published
- 2021
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31. Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteraction.
- Author
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Franz S, Pott F, Zillinger T, Schüler C, Dapa S, Fischer C, Passos V, Stenzel S, Chen F, Döhner K, Hartmann G, Sodeik B, Pessler F, Simmons G, Drexler JF, and Goffinet C
- Subjects
- Alphavirus pathogenicity, Alphavirus Infections metabolism, Alphavirus Infections virology, Cell Line, Cell Line, Tumor, Cell Membrane metabolism, Chikungunya Fever metabolism, Chikungunya Fever virology, Chikungunya virus pathogenicity, Endosomes metabolism, Humans, Membrane Proteins physiology, RNA-Binding Proteins physiology, Virus Internalization, Alphavirus Infections prevention & control, Chikungunya Fever prevention & control, Membrane Proteins metabolism, RNA-Binding Proteins metabolism
- Abstract
Interferon-induced transmembrane (IFITM) proteins restrict membrane fusion and virion internalization of several enveloped viruses. The role of IFITM proteins during alphaviral infection of human cells and viral counteraction strategies are insufficiently understood. Here, we characterized the impact of human IFITMs on the entry and spread of chikungunya virus and Mayaro virus and provide first evidence for a CHIKV-mediated antagonism of IFITMs. IFITM1, 2, and 3 restricted infection at the level of alphavirus glycoprotein-mediated entry, both in the context of direct infection and cell-to-cell transmission. Relocalization of normally endosomal IFITM3 to the plasma membrane resulted in loss of antiviral activity. rs12252-C, a naturally occurring variant of IFITM3 that may associate with severe influenza in humans, restricted CHIKV, MAYV, and influenza A virus infection as efficiently as wild-type IFITM3 Antivirally active IFITM variants displayed reduced cell surface levels in CHIKV-infected cells involving a posttranscriptional process mediated by one or several nonstructural protein(s) of CHIKV. Finally, IFITM3-imposed reduction of specific infectivity of nascent particles provides a rationale for the necessity of a virus-encoded counteraction strategy against this restriction factor., (© 2021 Franz et al.)
- Published
- 2021
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32. Absence of cGAS-mediated type I IFN responses in HIV-1-infected T cells.
- Author
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Elsner C, Ponnurangam A, Kazmierski J, Zillinger T, Jansen J, Todt D, Döhner K, Xu S, Ducroux A, Kriedemann N, Malassa A, Larsen PK, Hartmann G, Barchet W, Steinmann E, Kalinke U, Sodeik B, and Goffinet C
- Subjects
- Animals, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, Cells, Cultured, DNA, Viral physiology, Exodeoxyribonucleases genetics, Exodeoxyribonucleases metabolism, Herpesvirus 1, Human physiology, Host-Pathogen Interactions, Humans, Immunity, Innate, Interferon Regulatory Factor-3 metabolism, Mice, Nucleotidyltransferases genetics, Phosphoproteins genetics, Phosphoproteins metabolism, Phosphorylation, Species Specificity, Virus Replication, CD4-Positive T-Lymphocytes virology, HIV-1 physiology, Interferon Type I metabolism, Nucleotidyltransferases metabolism
- Abstract
The DNA sensor cGAS catalyzes the production of the cyclic dinucleotide cGAMP, resulting in type I interferon responses. We addressed the functionality of cGAS-mediated DNA sensing in human and murine T cells. Activated primary CD4
+ T cells expressed cGAS and responded to plasmid DNA by upregulation of ISGs and release of bioactive interferon. In mouse T cells, cGAS KO ablated sensing of plasmid DNA, and TREX1 KO enabled cells to sense short immunostimulatory DNA. Expression of IFIT1 and MX2 was downregulated and upregulated in cGAS KO and TREX1 KO T cell lines, respectively, compared to parental cells. Despite their intact cGAS sensing pathway, human CD4+ T cells failed to mount a reverse transcriptase (RT) inhibitor-sensitive immune response following HIV-1 infection. In contrast, infection of human T cells with HSV-1 that is functionally deficient for the cGAS antagonist pUL41 (HSV-1Δ UL41 N) resulted in a cGAS-dependent type I interferon response. In accordance with our results in primary CD4+ T cells, plasmid challenge or HSV-1Δ UL41 N inoculation of T cell lines provoked an entirely cGAS-dependent type I interferon response, including IRF3 phosphorylation and expression of ISGs. In contrast, no RT-dependent interferon response was detected following transduction of T cell lines with VSV-G-pseudotyped lentiviral or gammaretroviral particles. Together, T cells are capable to raise a cGAS-dependent cell-intrinsic response to both plasmid DNA challenge or inoculation with HSV-1Δ UL41 N. However, HIV-1 infection does not appear to trigger cGAS-mediated sensing of viral DNA in T cells, possibly by revealing viral DNA of insufficient quantity, length, and/or accessibility to cGAS., Competing Interests: Competing interest statement: W.B. is an employee of IFM Therapeutics., (Copyright © 2020 the Author(s). Published by PNAS.)- Published
- 2020
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33. Immune Sensing of Synthetic, Bacterial, and Protozoan RNA by Toll-like Receptor 8 Requires Coordinated Processing by RNase T2 and RNase 2.
- Author
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Ostendorf T, Zillinger T, Andryka K, Schlee-Guimaraes TM, Schmitz S, Marx S, Bayrak K, Linke R, Salgert S, Wegner J, Grasser T, Bauersachs S, Soltesz L, Hübner MP, Nastaly M, Coch C, Kettwig M, Roehl I, Henneke M, Hoerauf A, Barchet W, Gärtner J, Schlee M, Hartmann G, and Bartok E
- Subjects
- CRISPR-Cas Systems, Cell Line, Endoribonucleases immunology, Erythrocytes immunology, Erythrocytes parasitology, Escherichia coli chemistry, Escherichia coli immunology, Gene Editing methods, Humans, Listeria monocytogenes chemistry, Listeria monocytogenes immunology, Monocytes microbiology, Monocytes parasitology, Neutrophils microbiology, Neutrophils parasitology, Plasmodium falciparum chemistry, Plasmodium falciparum immunology, Primary Cell Culture, RNA Stability, RNA, Bacterial immunology, RNA, Protozoan immunology, Serratia marcescens chemistry, Serratia marcescens immunology, Staphylococcus aureus chemistry, Staphylococcus aureus immunology, Streptococcus chemistry, Streptococcus immunology, THP-1 Cells, Toll-Like Receptor 8 immunology, Endoribonucleases metabolism, Monocytes immunology, Neutrophils immunology, RNA, Bacterial metabolism, RNA, Protozoan metabolism, Toll-Like Receptor 8 metabolism
- Abstract
Human toll-like receptor 8 (TLR8) activation induces a potent T helper-1 (Th1) cell response critical for defense against intracellular pathogens, including protozoa. The receptor harbors two distinct binding sites, uridine and di- and/or trinucleotides, but the RNases upstream of TLR8 remain poorly characterized. We identified two endolysosomal endoribonucleases, RNase T2 and RNase 2, that act synergistically to release uridine from oligoribonucleotides. RNase T2 cleaves preferentially before, and RNase 2 after, uridines. Live bacteria, P. falciparum-infected red blood cells, purified pathogen RNA, and synthetic oligoribonucleotides all required RNase 2 and T2 processing to activate TLR8. Uridine supplementation restored RNA recognition in RNASE2
-/- or RNASET2-/- but not RNASE2-/- RNASET2-/- cells. Primary immune cells from RNase T2-hypomorphic patients lacked a response to bacterial RNA but responded robustly to small-molecule TLR8 ligands. Our data identify an essential function of RNase T2 and RNase 2 upstream of TLR8 and provide insight into TLR8 activation., Competing Interests: Declaration of Interests W.B. is an employee of IFM Therapeutics. The other authors declare no competing interests., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2020
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34. Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor.
- Author
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Passos V, Zillinger T, Casartelli N, Wachs AS, Xu S, Malassa A, Steppich K, Schilling H, Franz S, Todt D, Steinmann E, Sutter K, Dittmer U, Bohne J, Schwartz O, Barchet W, and Goffinet C
- Subjects
- CRISPR-Cas Systems, Cell Line, Cell Membrane drug effects, Cell Membrane metabolism, Gene Editing, Gene Expression Regulation, Gene Knockout Techniques, Genotype, HEK293 Cells, HIV Infections virology, Host-Pathogen Interactions physiology, Humans, Interferon-alpha, Membrane Proteins genetics, Nitriles, Pyrazoles pharmacology, Pyrimidines, T-Lymphocytes virology, Virion metabolism, nef Gene Products, Human Immunodeficiency Virus genetics, nef Gene Products, Human Immunodeficiency Virus metabolism, Anti-HIV Agents pharmacology, HIV-1 drug effects, Membrane Proteins metabolism, Membrane Proteins pharmacology
- Abstract
When expressed in virus-producing cells, the cellular multipass transmembrane protein SERINC5 reduces the infectivity of HIV-1 particles and is counteracted by HIV-1 Nef. Due to the unavailability of an antibody of sufficient specificity and sensitivity, investigation of SERINC5 protein expression and subcellular localization has been limited to heterologously expressed SERINC5. We generated, via CRISPR/Cas9-assisted gene editing, Jurkat T-cell clones expressing endogenous SERINC5 bearing an extracellularly exposed hemagglutinin (HA) epitope [Jurkat SERINC5 (iHA knock-in) T cells]. This modification enabled quantification of endogenous SERINC5 protein levels and demonstrated a predominant localization in lipid rafts. Interferon alpha (IFN-α) treatment enhanced cell surface levels of SERINC5 in a ruxolitinib-sensitive manner in the absence of modulation of mRNA and protein quantities. Parental and SERINC5 (iHA knock-in) T cells shared the ability to produce infectious wild-type HIV-1 but not an HIV-1 Δ nef mutant. SERINC5-imposed reduction of infectivity involved a modest reduction of virus fusogenicity. An association of endogenous SERINC5 protein with HIV-1 Δ nef virions was consistently detectable as a 35-kDa species, as opposed to heterologous SERINC5, which presented as a 51-kDa species. Nef-mediated functional counteraction did not correlate with virion exclusion of SERINC5, arguing for the existence of additional counteractive mechanisms of Nef that act on virus-associated SERINC5. In HIV-1-infected cells, Nef triggered the internalization of SERINC5 in the absence of detectable changes of steady-state protein levels. These findings establish new properties of endogenous SERINC5 expression and subcellular localization, challenge existing concepts of HIV-1 Nef-mediated antagonism of SERINC5, and uncover an unprecedented role of IFN-α in modulating SERINC5 through accumulation at the cell surface. IMPORTANCE SERINC5 is the long-searched-for antiviral factor that is counteracted by the HIV-1 accessory gene product Nef. Here, we engineered, via CRISPR/Cas9 technology, T-cell lines that express endogenous SERINC5 alleles tagged with a knocked-in HA epitope. This genetic modification enabled us to study basic properties of endogenous SERINC5 and to verify proposed mechanisms of HIV-1 Nef-mediated counteraction of SERINC5. Using this unique resource, we identified the susceptibility of endogenous SERINC5 protein to posttranslational modulation by type I IFNs and suggest uncoupling of Nef-mediated functional antagonism from SERINC5 exclusion from virions., (Copyright © 2019 Passos et al.)
- Published
- 2019
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35. Human TLR8 Senses RNA From Plasmodium falciparum -Infected Red Blood Cells Which Is Uniquely Required for the IFN-γ Response in NK Cells.
- Author
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Coch C, Hommertgen B, Zillinger T, Daßler-Plenker J, Putschli B, Nastaly M, Kümmerer BM, Scheunemann JF, Schumak B, Specht S, Schlee M, Barchet W, Hoerauf A, Bartok E, and Hartmann G
- Subjects
- Animals, Cell Line, Tumor, Disease Models, Animal, HEK293 Cells, Humans, Immunity, Innate immunology, Interleukin-12 immunology, Interleukin-18 immunology, Lymphocyte Activation immunology, Mice, Monocytes immunology, Plasmodium falciparum immunology, RNA, Protozoan genetics, THP-1 Cells, Toll-Like Receptor 7 immunology, Erythrocytes parasitology, Interferon-gamma immunology, Killer Cells, Natural immunology, Malaria, Falciparum immunology, RNA, Protozoan immunology, Toll-Like Receptor 8 immunology
- Abstract
During blood-stage malaria, the innate immune system initiates the production of pro-inflammatory cytokines, including IFN-γ, that are critical to host defense and responsible for severe disease. Nonetheless, the innate immune pathways activated during this process in human malaria remain poorly understood. Here, we identify TLR8 as an essential sensor of Plasmodium falciparum -infected red blood cells (iRBC). In human immune cells, iRBC and RNA purified from iRBC were detected by TLR8 but not TLR7 leading to IFN-γ induction in NK cells. While TLR7 and 9 have been shown to lead to IFN-γ in mice, our data demonstrate that TLR8 was the only TLR capable of inducing IFN-γ release in human immune cells. This unique capacity was mediated by the release of IL-12p70 and bioactive IL-18 from monocytes, the latter via a hitherto undescribed pathway. Altogether, our data are the first reported activation of TLR8 by protozoan RNA and demonstrate both the critical role of TLR8 in human blood-stage malaria and its unique functionality in the human immune system. Moreover, our study offers important evidence that mouse models alone may not be sufficient to describe the human innate immune response to malaria.
- Published
- 2019
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36. Targeted Nanoparticle Delivery of Bifunctional RIG-I Agonists to Pancreatic Cancer.
- Author
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Zillinger T and Hartmann G
- Subjects
- Humans, Nanoparticles, Pancreatic Neoplasms
- Published
- 2019
- Full Text
- View/download PDF
37. SIVcol Nef counteracts SERINC5 by promoting its proteasomal degradation but does not efficiently enhance HIV-1 replication in human CD4+ T cells and lymphoid tissue.
- Author
-
Kmiec D, Akbil B, Ananth S, Hotter D, Sparrer KMJ, Stürzel CM, Trautz B, Ayouba A, Peeters M, Yao Z, Stagljar I, Passos V, Zillinger T, Goffinet C, Sauter D, Fackler OT, and Kirchhoff F
- Subjects
- Animals, CD4-Positive T-Lymphocytes metabolism, Cells, Cultured, Colobus virology, HEK293 Cells, Humans, Jurkat Cells, Membrane Proteins genetics, Proteasome Endopeptidase Complex metabolism, Proteolysis, Simian Immunodeficiency Virus genetics, CD4-Positive T-Lymphocytes virology, Gene Products, nef physiology, HIV-1 physiology, Lymphoid Tissue virology, Membrane Proteins metabolism, Virus Replication genetics
- Abstract
SERINC5 is a host restriction factor that impairs infectivity of HIV-1 and other primate lentiviruses and is counteracted by the viral accessory protein Nef. However, the importance of SERINC5 antagonism for viral replication and cytopathicity remained unclear. Here, we show that the Nef protein of the highly divergent SIVcol lineage infecting mantled guerezas (Colobus guereza) is a potent antagonist of SERINC5, although it lacks the CD4, CD3 and CD28 down-modulation activities exerted by other primate lentiviral Nefs. In addition, SIVcol Nefs decrease CXCR4 cell surface expression, suppress TCR-induced actin remodeling, and counteract Colobus but not human tetherin. Unlike HIV-1 Nef proteins, SIVcol Nef induces efficient proteasomal degradation of SERINC5 and counteracts orthologs from highly divergent vertebrate species, such as Xenopus frogs and zebrafish. A single Y86F mutation disrupts SERINC5 and tetherin antagonism but not CXCR4 down-modulation by SIVcol Nef, while mutation of a C-proximal di-leucine motif has the opposite effect. Unexpectedly, the Y86F change in SIVcol Nef had little if any effect on viral replication and CD4+ T cell depletion in preactivated human CD4+ T cells and in ex vivo infected lymphoid tissue. However, SIVcol Nef increased virion infectivity up to 10-fold and moderately increased viral replication in resting peripheral blood mononuclear cells (PBMCs) that were first infected with HIV-1 and activated three or six days later. In conclusion, SIVcol Nef lacks several activities that are conserved in other primate lentiviruses and utilizes a distinct proteasome-dependent mechanism to counteract SERINC5. Our finding that evolutionarily distinct SIVcol Nefs show potent anti-SERINC5 activity supports a relevant role of SERINC5 antagonism for viral fitness in vivo. Our results further suggest this Nef function is particularly important for virion infectivity under conditions of limited CD4+ T cell activation., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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38. Suppressive oligodeoxynucleotides containing TTAGGG motifs inhibit cGAS activation in human monocytes.
- Author
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Steinhagen F, Zillinger T, Peukert K, Fox M, Thudium M, Barchet W, Putensen C, Klinman D, Latz E, and Bode C
- Subjects
- Cell Line, Cytosol, DNA genetics, Exodeoxyribonucleases genetics, HEK293 Cells, Humans, Lupus Erythematosus, Systemic genetics, Lupus Erythematosus, Systemic immunology, Phosphoproteins genetics, Telomere genetics, Interferon Type I biosynthesis, Monocytes immunology, Nucleotide Motifs genetics, Nucleotidyltransferases antagonists & inhibitors, Oligodeoxyribonucleotides genetics, Oligonucleotides genetics
- Abstract
Type I interferon (IFN) is a critical mediator of autoimmune diseases such as systemic lupus erythematosus (SLE) and Aicardi-Goutières Syndrome (AGS). The recently discovered cyclic-GMP-AMP (cGAMP) synthase (cGAS) induces the production of type I IFN in response to cytosolic DNA and is potentially linked to SLE and AGS. Suppressive oligodeoxynucleotides (ODN) containing repetitive TTAGGG motifs present in mammalian telomeres have proven useful in the treatment of autoimmune diseases including SLE. In this study, we demonstrate that the suppressive ODN A151 effectively inhibits activation of cGAS in response to cytosolic DNA, thereby inhibiting type I IFN production by human monocytes. In addition, A151 abrogated cGAS activation in response to endogenous accumulation of DNA using TREX1-deficient monocytes. We demonstrate that A151 prevents cGAS activation in a manner that is competitive with DNA. This suppressive activity of A151 was dependent on both telomeric sequence and phosphorothioate backbone. To our knowledge this report presents the first cGAS inhibitor capable of blocking self-DNA. Collectively, these findings might lead to the development of new therapeutics against IFN-driven pathologies due to cGAS activation., (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2018
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39. G-rich DNA-induced stress response blocks type-I-IFN but not CXCL10 secretion in monocytes.
- Author
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Herzner AM, Wolter S, Zillinger T, Schmitz S, Barchet W, Hartmann G, Bartok E, and Schlee M
- Subjects
- Cells, Cultured, Chemokine CXCL10 genetics, GC Rich Sequence, Gene Expression Regulation immunology, Humans, Immunity, Innate drug effects, Immunologic Factors pharmacology, Interferon-beta genetics, Interferon-beta metabolism, Oligonucleotides pharmacology, Protein Biosynthesis, Stress, Physiological, Chemokine CXCL10 metabolism, Interferon-alpha metabolism, Leukocytes, Mononuclear metabolism
- Abstract
Excessive inflammation can cause damage to host cells and tissues. Thus, the secretion of inflammatory cytokines is tightly regulated at transcriptional, post-transcriptional and post-translational levels and influenced by cellular stress responses, such as endoplasmic reticulum (ER) stress or apoptosis. Here, we describe a novel type of post-transcriptional regulation of the type-I-IFN response that was induced in monocytes by cytosolic transfection of a short immunomodulatory DNA (imDNA), a G-tetrad forming CpG-free derivative of the TLR9 agonist ODN2216. When co-transfected with cytosolic nucleic acid stimuli (DNA or 3P-dsRNA), imDNA induced caspase-3 activation, translational shutdown and upregulation of stress-induced genes. This stress response inhibited the type-I-IFN induction at the translational level. By contrast, the induction of most type-I-IFN-associated chemokines, including Chemokine (C-X-C Motif) Ligand (CXCL)10 was not affected, suggesting a differential translational regulation of chemokines and type-I-IFN. Pan-caspase inhibitors could restore IFN-β secretion, yet, strikingly, caspase inhibition did not restore global translation but instead induced a compensatory increase in the transcription of IFN-β but not CXCL10. Altogether, our data provide evidence for a differential regulation of cytokine release at both transcriptional and post-transcriptional levels which suppresses type-I-IFN induction yet allows for CXCL10 secretion during imDNA-induced cellular stress., Competing Interests: G.H. is co-founder and shareholder of the Rigontec GmbH. All other authors declare no competing financial interests.
- Published
- 2016
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40. cGAS-Mediated Innate Immunity Spreads Intercellularly through HIV-1 Env-Induced Membrane Fusion Sites.
- Author
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Xu S, Ducroux A, Ponnurangam A, Vieyres G, Franz S, Müsken M, Zillinger T, Malassa A, Ewald E, Hornung V, Barchet W, Häussler S, Pietschmann T, and Goffinet C
- Subjects
- Biological Transport, Cell Line, DNA, Viral metabolism, Humans, Immunity, Innate, Membrane Proteins metabolism, T-Lymphocytes immunology, HIV-1 immunology, Interferon Type I metabolism, Macrophages immunology, Membrane Fusion, Nucleotides, Cyclic metabolism, Nucleotidyltransferases metabolism, T-Lymphocytes virology
- Abstract
Upon sensing cytoplasmic retroviral DNA in infected cells, cyclic GMP-AMP (cGAMP) synthase (cGAS) produces the cyclic dinucleotide cGAMP, which activates STING to trigger a type I interferon (IFN) response. We find that membrane fusion-inducing contact between donor cells expressing the HIV envelope (Env) and primary macrophages endogenously expressing the HIV receptor CD4 and coreceptor enable intercellular transfer of cGAMP. This cGAMP exchange results in STING-dependent antiviral IFN responses in target macrophages and protection from HIV infection. Furthermore, under conditions allowing cell-to-cell transmission of HIV-1, infected primary T cells, but not cell-free virions, deliver cGAMP to autologous macrophages through HIV-1 Env and CD4/coreceptor-mediated membrane fusion sites and induce a STING-dependent, but cGAS-independent, IFN response in target cells. Collectively, these findings identify an infection-specific mode of horizontal transfer of cGAMP between primary immune cells that may boost antiviral responses, particularly in infected tissues in which cell-to-cell transmission of virions exceeds cell-free infection., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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41. Sequence-specific activation of the DNA sensor cGAS by Y-form DNA structures as found in primary HIV-1 cDNA.
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Herzner AM, Hagmann CA, Goldeck M, Wolter S, Kübler K, Wittmann S, Gramberg T, Andreeva L, Hopfner KP, Mertens C, Zillinger T, Jin T, Xiao TS, Bartok E, Coch C, Ackermann D, Hornung V, Ludwig J, Barchet W, Hartmann G, and Schlee M
- Subjects
- Animals, Cell Line, Cells, Cultured, DNA, Complementary genetics, DNA, Complementary immunology, DNA, Viral genetics, HEK293 Cells, Humans, Immunization, Mice, DNA, Complementary chemistry, DNA, Viral chemistry, DNA, Viral immunology, HIV-1 genetics, HIV-1 immunology, Interferon-alpha immunology, Nucleotidyltransferases genetics
- Abstract
Cytosolic DNA that emerges during infection with a retrovirus or DNA virus triggers antiviral type I interferon responses. So far, only double-stranded DNA (dsDNA) over 40 base pairs (bp) in length has been considered immunostimulatory. Here we found that unpaired DNA nucleotides flanking short base-paired DNA stretches, as in stem-loop structures of single-stranded DNA (ssDNA) derived from human immunodeficiency virus type 1 (HIV-1), activated the type I interferon-inducing DNA sensor cGAS in a sequence-dependent manner. DNA structures containing unpaired guanosines flanking short (12- to 20-bp) dsDNA (Y-form DNA) were highly stimulatory and specifically enhanced the enzymatic activity of cGAS. Furthermore, we found that primary HIV-1 reverse transcripts represented the predominant viral cytosolic DNA species during early infection of macrophages and that these ssDNAs were highly immunostimulatory. Collectively, our study identifies unpaired guanosines in Y-form DNA as a highly active, minimal cGAS recognition motif that enables detection of HIV-1 ssDNA.
- Published
- 2015
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42. Exonuclease TREX1 also Has a Sweet Tooth.
- Author
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Barchet W and Zillinger T
- Subjects
- Animals, Humans, Cytosol enzymology, Exodeoxyribonucleases metabolism, Hexosyltransferases metabolism, Membrane Proteins metabolism, Phosphoproteins metabolism
- Abstract
TREX1 regulates innate immune responses by counteracting DNA accumulation in the cytosol. In this issue of Immunity, Hasan et al. (2015) show that TREX1 also safeguards the cell against free glycan build-up in the endoplasmic reticulum, thereby preventing glycan-induced inflammation., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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43. A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2'O-Methylated Self RNA.
- Author
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Schuberth-Wagner C, Ludwig J, Bruder AK, Herzner AM, Zillinger T, Goldeck M, Schmidt T, Schmid-Burgk JL, Kerber R, Wolter S, Stümpel JP, Roth A, Bartok E, Drosten C, Coch C, Hornung V, Barchet W, Kümmerer BM, Hartmann G, and Schlee M
- Subjects
- Amino Acid Sequence, Animals, Cells, Cultured, DEAD Box Protein 58, Enzyme Activation genetics, Enzyme Activation immunology, Histidine genetics, Humans, Methylation, Methyltransferases genetics, Mice, Protein Structure, Tertiary, RNA chemistry, RNA immunology, RNA, Viral immunology, Receptors, Immunologic, Yellow fever virus genetics, DEAD-box RNA Helicases genetics, Immune Tolerance genetics, RNA genetics, RNA Processing, Post-Transcriptional genetics, Yellow fever virus enzymology
- Abstract
The cytosolic helicase retinoic acid-inducible gene-I (RIG-I) initiates immune responses to most RNA viruses by detecting viral 5'-triphosphorylated RNA (pppRNA). Although endogenous mRNA is also 5'-triphosphorylated, backbone modifications and the 5'-ppp-linked methylguanosine ((m7)G) cap prevent immunorecognition. Here we show that the methylation status of endogenous capped mRNA at the 5'-terminal nucleotide (N1) was crucial to prevent RIG-I activation. Moreover, we identified a single conserved amino acid (H830) in the RIG-I RNA binding pocket as the mediator of steric exclusion of N1-2'O-methylated RNA. H830A alteration (RIG-I(H830A)) restored binding of N1-2'O-methylated pppRNA. Consequently, endogenous mRNA activated the RIG-I(H830A) mutant but not wild-type RIG-I. Similarly, knockdown of the endogenous N1-2'O-methyltransferase led to considerable RIG-I stimulation in the absence of exogenous stimuli. Studies involving yellow-fever-virus-encoded 2'O-methyltransferase and RIG-I(H830A) revealed that viruses exploit this mechanism to escape RIG-I. Our data reveal a new role for cap N1-2'O-methylation in RIG-I tolerance of self-RNA., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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44. Antiviral immunity via RIG-I-mediated recognition of RNA bearing 5'-diphosphates.
- Author
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Goubau D, Schlee M, Deddouche S, Pruijssers AJ, Zillinger T, Goldeck M, Schuberth C, Van der Veen AG, Fujimura T, Rehwinkel J, Iskarpatyoti JA, Barchet W, Ludwig J, Dermody TS, Hartmann G, and Reis e Sousa C
- Subjects
- Animals, Base Pairing, Base Sequence, DEAD Box Protein 58, Female, Genome, Viral genetics, Male, Mice, RNA, Viral genetics, Reoviridae physiology, DEAD-box RNA Helicases metabolism, Diphosphates metabolism, Immunity, Innate, RNA, Viral chemistry, RNA, Viral metabolism, Reoviridae genetics, Reoviridae immunology
- Abstract
Mammalian cells possess mechanisms to detect and defend themselves from invading viruses. In the cytosol, the RIG-I-like receptors (RLRs), RIG-I (retinoic acid-inducible gene I; encoded by DDX58) and MDA5 (melanoma differentiation-associated gene 5; encoded by IFIH1) sense atypical RNAs associated with virus infection. Detection triggers a signalling cascade via the adaptor MAVS that culminates in the production of type I interferons (IFN-α and β; hereafter IFN), which are key antiviral cytokines. RIG-I and MDA5 are activated by distinct viral RNA structures and much evidence indicates that RIG-I responds to RNAs bearing a triphosphate (ppp) moiety in conjunction with a blunt-ended, base-paired region at the 5'-end (reviewed in refs 1, 2, 3). Here we show that RIG-I also mediates antiviral responses to RNAs bearing 5'-diphosphates (5'pp). Genomes from mammalian reoviruses with 5'pp termini, 5'pp-RNA isolated from yeast L-A virus, and base-paired 5'pp-RNAs made by in vitro transcription or chemical synthesis, all bind to RIG-I and serve as RIG-I agonists. Furthermore, a RIG-I-dependent response to 5'pp-RNA is essential for controlling reovirus infection in cultured cells and in mice. Thus, the minimal determinant for RIG-I recognition is a base-paired RNA with 5'pp. Such RNAs are found in some viruses but not in uninfected cells, indicating that recognition of 5'pp-RNA, like that of 5'ppp-RNA, acts as a powerful means of self/non-self discrimination by the innate immune system.
- Published
- 2014
- Full Text
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45. Binding-pocket and lid-region substitutions render human STING sensitive to the species-specific drug DMXAA.
- Author
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Gao P, Zillinger T, Wang W, Ascano M, Dai P, Hartmann G, Tuschl T, Deng L, Barchet W, and Patel DJ
- Subjects
- Amino Acid Sequence, Animals, Antineoplastic Agents chemistry, Antineoplastic Agents metabolism, Antineoplastic Agents therapeutic use, Binding Sites, Chemokines metabolism, Crystallography, X-Ray, HEK293 Cells, Humans, Interferon Type I metabolism, Membrane Proteins chemistry, Membrane Proteins genetics, Mice, Molecular Dynamics Simulation, Molecular Sequence Data, Mutagenesis, Site-Directed, Neoplasms drug therapy, Protein Structure, Tertiary, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Sequence Alignment, Xanthones chemistry, Xanthones therapeutic use, Membrane Proteins metabolism, Xanthones metabolism
- Abstract
The drug DMXAA (5,6-dimethylxanthenone-4-acetic acid) showed therapeutic promise against solid tumors in mouse models but subsequently failed in human clinical trials. DMXAA was later discovered to activate mouse, but not human, STING, an adaptor protein in the cyclic dinucleotide cGAMP-mediated signaling pathway, inducing type I interferon expression. To facilitate the development of compounds that target human STING, we combined structural, biophysical, and cellular assays to study mouse and human chimeric proteins and their interaction with DMXAA. We identified a single substitution (G230I) that enables a DMXAA-induced conformational transition of hSTING from an inactive "open" to an active "closed" state. We also identified a substitution within the binding pocket (Q266I) that cooperates with G230I and the previously identified S162A binding-pocket point substitution, rendering hSTING highly sensitive to DMXAA. These findings should facilitate the reciprocal engineering of DMXAA analogs that bind and stimulate wild-type hSTING and their exploitation for vaccine-adjuvant and anticancer drug development., (Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
46. Host-cell sensors for Plasmodium activate innate immunity against liver-stage infection.
- Author
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Liehl P, Zuzarte-Luís V, Chan J, Zillinger T, Baptista F, Carapau D, Konert M, Hanson KK, Carret C, Lassnig C, Müller M, Kalinke U, Saeed M, Chora AF, Golenbock DT, Strobl B, Prudêncio M, Coelho LP, Kappe SH, Superti-Furga G, Pichlmair A, Vigário AM, Rice CM, Fitzgerald KA, Barchet W, and Mota MM
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Animals, Blotting, Western, DEAD-box RNA Helicases immunology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Gene Expression Profiling, Green Fluorescent Proteins, Immunohistochemistry, Interferon Regulatory Factor-3 metabolism, Interferon Regulatory Factor-7 metabolism, Interferon-Induced Helicase, IFIH1, Liver immunology, Luciferases, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microarray Analysis, Oligonucleotides genetics, Plasmodium genetics, Real-Time Polymerase Chain Reaction, Statistics, Nonparametric, Immunity, Innate immunology, Interferon Type I immunology, Liver parasitology, Plasmodium immunology, Signal Transduction immunology
- Abstract
Before they infect red blood cells and cause malaria, Plasmodium parasites undergo an obligate and clinically silent expansion phase in the liver that is supposedly undetected by the host. Here, we demonstrate the engagement of a type I interferon (IFN) response during Plasmodium replication in the liver. We identified Plasmodium RNA as a previously unrecognized pathogen-associated molecular pattern (PAMP) capable of activating a type I IFN response via the cytosolic pattern recognition receptor Mda5. This response, initiated by liver-resident cells through the adaptor molecule for cytosolic RNA sensors, Mavs, and the transcription factors Irf3 and Irf7, is propagated by hepatocytes in an interferon-α/β receptor-dependent manner. This signaling pathway is critical for immune cell-mediated host resistance to liver-stage Plasmodium infection, which we find can be primed with other PAMPs, including hepatitis C virus RNA. Together, our results show that the liver has sensor mechanisms for Plasmodium that mediate a functional antiparasite response driven by type I IFN.
- Published
- 2014
- Full Text
- View/download PDF
47. Middle East respiratory syndrome coronavirus accessory protein 4a is a type I interferon antagonist.
- Author
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Niemeyer D, Zillinger T, Muth D, Zielecki F, Horvath G, Suliman T, Barchet W, Weber F, Drosten C, and Müller MA
- Subjects
- Amino Acid Sequence, Coronavirus growth & development, Coronavirus Infections immunology, Coronavirus Infections metabolism, Humans, Middle East, Molecular Sequence Data, RNA, Double-Stranded metabolism, Sequence Homology, Amino Acid, Severe Acute Respiratory Syndrome immunology, Severe Acute Respiratory Syndrome metabolism, Coronavirus pathogenicity, Coronavirus Infections virology, Immunity, Innate immunology, Interferon Type I antagonists & inhibitors, Severe Acute Respiratory Syndrome virology, Viral Regulatory and Accessory Proteins metabolism
- Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory infection with as yet unclear epidemiology. We previously showed that MERS-CoV counteracts parts of the innate immune response in human bronchiolar cells. Here we analyzed accessory proteins 3, 4a, 4b, and 5 for their abilities to inhibit the type I interferon response. Accessory protein 4a was found to block interferon induction at the level of melanoma differentiation-associated protein 5 (MDA5) activation presumably by direct interaction with double-stranded RNA.
- Published
- 2013
- Full Text
- View/download PDF
48. Oxidative damage of DNA confers resistance to cytosolic nuclease TREX1 degradation and potentiates STING-dependent immune sensing.
- Author
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Gehrke N, Mertens C, Zillinger T, Wenzel J, Bald T, Zahn S, Tüting T, Hartmann G, and Barchet W
- Subjects
- Animals, Cells, Cultured, DNA Repair, Guanosine analogs & derivatives, Guanosine metabolism, Humans, Interferon Type I, Lupus Erythematosus, Systemic genetics, Lupus Erythematosus, Systemic immunology, Lupus Erythematosus, Systemic metabolism, Mice, Mice, Inbred C57BL, Neutrophils metabolism, Oxidation-Reduction, Oxidative Stress, Reactive Oxygen Species metabolism, DNA metabolism, DNA Damage, Exodeoxyribonucleases metabolism, Membrane Proteins metabolism, Phosphoproteins metabolism
- Abstract
Immune sensing of DNA is critical for antiviral immunity but can also trigger autoimmune diseases such as lupus erythematosus (LE). Here we have provided evidence for the involvement of a damage-associated DNA modification in the detection of cytosolic DNA. The oxidized base 8-hydroxyguanosine (8-OHG), a marker of oxidative damage in DNA, potentiated cytosolic immune recognition by decreasing its susceptibility to 3' repair exonuclease 1 (TREX1)-mediated degradation. Oxidizative modifications arose physiologically in pathogen DNA during lysosomal reactive oxygen species (ROS) exposure, as well as in neutrophil extracellular trap (NET) DNA during the oxidative burst. 8-OHG was also abundant in UV-exposed skin lesions of LE patients and colocalized with type I interferon (IFN). Injection of oxidized DNA in the skin of lupus-prone mice induced lesions that closely matched respective lesions in patients. Thus, oxidized DNA represents a prototypic damage-associated molecular pattern (DAMP) with important implications for infection, sterile inflammation, and autoimmunity., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
49. Structure-function analysis of STING activation by c[G(2',5')pA(3',5')p] and targeting by antiviral DMXAA.
- Author
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Gao P, Ascano M, Zillinger T, Wang W, Dai P, Serganov AA, Gaffney BL, Shuman S, Jones RA, Deng L, Hartmann G, Barchet W, Tuschl T, and Patel DJ
- Subjects
- Animals, Crystallography, X-Ray, Cyclic GMP metabolism, Humans, Interferon Regulatory Factor-3 metabolism, Interferon Type I metabolism, Membrane Proteins antagonists & inhibitors, Membrane Proteins genetics, Mice, Models, Molecular, Mutagenesis, Protein Conformation, Signal Transduction, Structure-Activity Relationship, Antiviral Agents pharmacology, Membrane Proteins chemistry, Membrane Proteins metabolism, Nucleotides, Cyclic metabolism, Xanthones pharmacology
- Abstract
Binding of dsDNA by cyclic GMP-AMP (cGAMP) synthase (cGAS) triggers formation of the metazoan second messenger c[G(2',5')pA(3',5')p], which binds the signaling protein STING with subsequent activation of the interferon (IFN) pathway. We show that human hSTING(H232) adopts a "closed" conformation upon binding c[G(2',5')pA(3',5')p] and its linkage isomer c[G(2',5')pA(2',5')p], as does mouse mSting(R231) on binding c[G(2',5')pA(3',5')p], c[G(3',5')pA(3',5')p] and the antiviral agent DMXAA, leading to similar "closed" conformations. Comparing hSTING to mSting, 2',5'-linkage-containing cGAMP isomers were more specific triggers of the IFN pathway compared to the all-3',5'-linkage isomer. Guided by structural information, we identified a unique point mutation (S162A) placed within the cyclic-dinucleotide-binding site of hSTING that rendered it sensitive to the otherwise mouse-specific drug DMXAA, a conclusion validated by binding studies. Our structural and functional analysis highlights the unexpected versatility of STING in the recognition of natural and synthetic ligands within a small-molecule pocket created by the dimerization of STING., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
50. Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.
- Author
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Gao P, Ascano M, Wu Y, Barchet W, Gaffney BL, Zillinger T, Serganov AA, Liu Y, Jones RA, Hartmann G, Tuschl T, and Patel DJ
- Subjects
- 2',5'-Oligoadenylate Synthetase chemistry, Adenosine Triphosphate metabolism, Amino Acid Sequence, Animals, Crystallography, X-Ray, DNA chemistry, DNA metabolism, Guanosine Triphosphate metabolism, Humans, Mice, Models, Chemical, Models, Molecular, Molecular Sequence Data, Nucleotidyltransferases metabolism, Sequence Alignment, Dinucleoside Phosphates metabolism, Nucleotides, Cyclic metabolism, Nucleotidyltransferases chemistry, Second Messenger Systems
- Abstract
Recent studies identified cyclic GMP-AMP (cGAMP) as a metazoan second messenger triggering an interferon response. cGAMP is generated from GTP and ATP by cytoplasmic dsDNA sensor cGAMP synthase (cGAS). We combined structural, chemical, biochemical, and cellular assays to demonstrate that this second messenger contains G(2',5')pA and A(3',5')pG phosphodiester linkages, designated c[G(2',5')pA(3',5')p]. We show that, upon dsDNA binding, cGAS is activated through conformational transitions, resulting in formation of a catalytically competent and accessible nucleotide-binding pocket for generation of c[G(2',5')pA(3',5')p]. We demonstrate that cyclization occurs in a stepwise manner through initial generation of 5'-pppG(2',5')pA prior to cyclization to c[G(2',5')pA(3',5')p], with the latter positioned precisely in the catalytic pocket. Mutants of cGAS dsDNA-binding or catalytic pocket residues exhibit reduced or abrogated activity. Our studies have identified c[G(2',5')pA(3',5')p] as a founding member of a family of metazoan 2',5'-containing cyclic heterodinucleotide second messengers distinct from bacterial 3',5' cyclic dinucleotides., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
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