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In China's coal mine backfill mining projects, certain mines demonstrate an extensive filling distance that exceeds 3000 m. However, there are few researches concerning the theoretical underpinnings for slurry long-distance pipeline transportation. Gangue particles suspending in filling slurry is the prerequisite for successful long-distance pipeline transportation. This paper conducts theoretical researches on the heightened sensitivity issue of particle settling within coal-based solid waste filling slurry (CSWFS) for long-distance pipeline transportation, which can easily lead to unstable slurry concentration then pipe blockage or bursting accidents. The mechanical model for particle settling and critical non-settling particle diameter for long-distance pipeline transportation are developed based on the ''solid-liquid two-phase carrier suspension fluid'', and reveal the intrinsical mechanism of particle settling within CSWFS for long-distance pipeline transportation. Through the calculation of the mechanical model for critical non-settling particle diameter and particle settling, coupled with the particle diameter screening experiment, the value of critical non-settling particle diameter is determined to be 0.0067 m, and the density of solid-liquid two-phase carrier suspension fluid is 1712.37 kg/m3. The CSWFS is classified as a semi-stable slurry, lacking time-dependent suspending state required for long-distance pipeline transportation, which could lead to pipe blockage accidents. Further research into effective methods is urgently required to ensure adequate safety and adaptability for long-distance pipeline transportation of CSWFS.

Abstract Background Dancers have a higher prevalence of hallux valgus (HV) than the general population. However, no reports specifically addressing treatment strategies for HV in dancers, or their effectiveness have been published. This study aimed to determine the effects of 4 weeks of foot exercises on subjective clinical outcomes, the HV angle, and change of foot plantar pressure during specific dance techniques. Methods Thirteen female elite adolescent dancesport athletes (age: 16.4 ± 3.3 years) completed the program. The HV angle was measured based on foot photographs. Clinical assessments were performed using the visual analog scale for first metatarsophalangeal (1st MTP) joint pain, and the Foot and Ankle Outcome Score. The changes in plantar pressure during two dance techniques (demi-pointe and plié) were measured. The plantar pressure was measured in six areas of the foot. All of these parameters were compared before and after the intervention by using chi-square tests and Student’s paired t-test (statistical significance: p

Abstract The rapid expansion of information technology and the intensification of population aging are two prominent features of contemporary societal development. Investigating older adults’ acceptance and use of technology is key to facilitating their integration into an information-driven society. Given this context, the technology acceptance of older adults has emerged as a prioritized research topic, attracting widespread attention in the academic community. However, existing research remains fragmented and lacks a systematic framework. To address this gap, we employed bibliometric methods, utilizing the Web of Science Core Collection to conduct a comprehensive review of literature on older adults’ technology acceptance from 2013 to 2023. Utilizing VOSviewer and CiteSpace for data assessment and visualization, we created knowledge mappings of research on older adults’ technology acceptance. Our study employed multidimensional methods such as co-occurrence analysis, clustering, and burst analysis to: (1) reveal research dynamics, key journals, and domains in this field; (2) identify leading countries, their collaborative networks, and core research institutions and authors; (3) recognize the foundational knowledge system centered on theoretical model deepening, emerging technology applications, and research methods and evaluation, uncovering seminal literature and observing a shift from early theoretical and influential factor analyses to empirical studies focusing on individual factors and emerging technologies; (4) moreover, current research hotspots are primarily in the areas of factors influencing technology adoption, human-robot interaction experiences, mobile health management, and aging-in-place technology, highlighting the evolutionary context and quality distribution of research themes. Finally, we recommend that future research should deeply explore improvements in theoretical models, long-term usage, and user experience evaluation. Overall, this study presents a clear framework of existing research in the field of older adults’ technology acceptance, providing an important reference for future theoretical exploration and innovative applications.

Abstract The upper respiratory tract is the initial site of SARS-CoV-2 infection. Nasal spike-specific secretory immunoglobulin A (sIgA) correlates with protection against Omicron breakthrough infection. We report that intranasal vaccination using human adenovirus serotype 5 (Ad5) vectored Omicron spike in people who previously vaccinated with ancestral vaccine could induce robust neutralizing sIgA in the nasal passage. Nasal sIgA was predominantly present in dimeric and multimeric forms and accounted for nearly 40% of total proteins in nasal mucosal lining fluids (NMLFs). A low-level IgG could also be detected in NMLFs but not IgM, IgD, and IgE. After a complete nasal wash, sIgA in the nasal passage could be replenished rapidly within a few hours. A comparison of purified paired serum IgA, serum IgG, and nasal sIgA from the same individuals showed that sIgA was up to 3-logs more potent than serum antibodies in binding to spikes and in neutralizing Omicron subvariants. Serum IgG and IgA failed to neutralize XBB and BA.2.86, while nasal sIgA retained potent neutralization against these newly emerged variants. Further analysis showed that sIgA was more effective than IgG or IgA in blocking spike-mediated cell-to-cell transmission and protecting hACE2 mice from XBB challenge. Using a sIgA monoclonal antibody as a reference, we estimated that the total nasal sIgA contains about 2.6–3.9% spike-specific sIgA in NMLFs collected approximately one month after intranasal vaccination. Our study provided insights for developing intranasal vaccines that can induce sIgA to build an effective and mutation-resistant first-line immune barrier against constantly emerging variants.

IntroductionThis study examined the relationship between ball release points and pitching performance among professional baseball pitchers, with a focus on variability.MethodsWe used open-source data to compare ball release point variability between Major League Baseball (MLB) and Minor League Baseball (MiLB) players. The relationship between pitching performance and variability was analyzed using multiple regression analysis.ResultsMLB players exhibited smaller ball release point variability compared to MiLB players. The analysis showed that pitching performance was strongly related to ball release point variability, especially in the horizontal direction on the coronal plane. Horizontal ball release point variability was most strongly related to strikeout ability among pitching performances.DiscussionThese results suggest that reducing horizontal ball release point variability may improve pitching performance, particularly by increasing strikeouts and reducing home runs allowed. This study provides a data-driven approach to understanding the mechanics of pitching and can be applied to the development of advanced training methods and technical solutions aimed at improving pitching performance in baseball players.

IntroductionAlmost all cases of malignant acanthosis nigricans with oral manifestations occurred in older age groups. Here, we report a case of malignant acanthosis nigricans in a young female presented with chief complaints of oral mucosal hyperplasia, who had previously been diagnosed with allergies.Case presentationA 30-year-old female developed oral hyperplasia and rash following seafood consumption, with subsequent resolution of the rash but persistent oral lesions and the appearance of pigmentation on her hands, neck, and axillae. Clinical examination revealed papillomatosis, hyperemia, and velvety hyperplasia in the oral cavity. Biopsy results confirmed papillary hyperplasia. Despite the patient’s belief in good health, she was advised to undergo further systemic examinations. Elevated serum tumor markers and histologic analysis of an endoscopic biopsy confirmed gastric cancer with duodenal infiltration, leading to the diagnosis of malignant acanthosis nigricans. Unfortunately, the patient passed away due to heart failure during chemotherapy treatment.ConclusionsThe majority of patients with malignant acanthosis nigricans present with oral lesions before the underlying malignancy is detected, emphasizing the importance of timely comprehensive systemic examination. Furthermore, our case suggests that age may not be a restrictive factor for the development of malignant acanthosis nigricans, and the presence of a rash can potentially obscure the cutaneous manifestations associated with this condition.

Abstract Background This study aimed to determine the risk factors of hallux valgus angle among preprofessional adolescent dancesport athletes. Methods A total of 275 athletes, (73 males and 202 females) aged between the ages of 11 and 18 years, participated in this study. A cross‐sectional questionnaire was used to survey their demographic characteristics (sex and age), training information (starting age, weekly training time, and athletic career [number of years of training at this specific dancesport school]), and measured their height and weight. The hallux valgus angle was measured based on foot photographs. The chi‐square test was used to compare the difference with prevalence of hallux valgus between male and female athletes. A normal distribution test was performed, and based on the test results, unpaired t‐test and multiple logistic regression were conducted to identify training factors for the hallux valgus in this cohort. Results Chi‐square test showed higher prevalence of hallux valgus in female elite adolescent dancesport athletes than males. The t‐test results did not show any significant differences between the hallux valgus group and non‐hallux valgus groups with start age, athletic career, and weekly training time. Multiple logistic regression analysis with hallux valgus as the dependent variable revealed that the female sex was a strong predictor of a higher prevalence of hallux valgus (odds ratio [OR]: 3.954, 95% confidence interval 95% CI: 2.193–7.131, and p

The geographical origin was one of the important factors affecting the quality of hawthorn. To discriminate the geographical origin of hawthorn rapidly and nondestructively, hawthorns from five different provincial production areas were used as samples, and visible-shortwave infrared (410~2500 nm) band hyperspectral data were obtained for the pedicel face (G), side (C), and bottom (D) of each sample by using a near-infrared hyperspectral imaging system. Partial least squares discriminant analysis (PLS-DA), support vector machine (SVM), and random forests (RF) classification models were built by multivariate scattering correction (MSC), first derivative (D1), SG smoothing (Savitzky-Golay, SG), and standard normal transform (SNV) four preprocessing methods. The results showed that the D-D1-SVM model discriminated optimally, with 100% accuracy in both the training and prediction sets. To simplify the model, successive projections algorithm (SPA) and competitive adaptive reweighted sampling algorithm (CARS) were applied to select feature wavelength. The multivariate data analysis revealed that the D-SPA-SVM model had the best performance, with an accuracy of 95.2% and 93% for the training and prediction sets, respectively. This study could provide technical support for rapid and non-destructive identification of hawthorn origin.

Abstract Background Anhui Province is currently facing an increase in imported malaria cases as a result of globalization and international travel. In response, Anhui Province has implemented a comprehensive adaptive framework to effectively address this threat. Methods This study collected surveillance data from 2012 to 2022 in Anhui Province. Descriptive statistics were used to analyze the epidemiological characteristics of imported malaria cases. Additionally, multivariate logistic regression was employed to identify factors associated with severe malaria. Documents were reviewed to document the evolution of the adaptive framework designed to combat imported malaria. The effectiveness of the adaptive framework was evaluated based on the rates of timely medical visits, timely diagnosis, and species identification. Results During the study period, a total of 1008 imported malaria cases were reported across 77 out of 105 counties in Anhui Province, representing a coverage of 73.33%. It was found that 10.52% of imported cases went undiagnosed for more than seven days after onset. The multivariate analysis revealed several potential risk factors for severe malaria, including increasing age (OR = 1.049, 95%CI:1.015–1.083), occupation (waitperson vs. worker, OR = 2.698, 95%CI:1.054–6.906), a longer time interval between onset and the initial medical visit (OR = 1.061, 95%CI:1.011–1.114), and misdiagnosis during the first medical visit (OR = 5.167, 95%CI:2.535–10.533). Following the implementation of the adaptive framework, the rates of timely medical visits, timely diagnosis, and species identification reached 100.00%, 78.57%, and 100.00%, respectively. Conclusions Anhui Province has successfully developed and implemented an adaptive framework for addressing imported malaria, focusing on robust surveillance, prompt diagnosis, and standardized treatment. The experiences gained from this initiative can serve as a valuable reference for other non-endemic areas.

The continuous emergence of highly immune-evasive SARS-CoV-2 variants has challenged vaccine efficacy. A vaccine that can provide broad protection is desirable. We evaluated the immunogenicity of a series of monovalent and bivalent adenovirus-vectored vaccines containing the spikes of Wildtype (WT), Beta, Delta, Omicron subvariants BA.1, BA.2, BA.2.12.1, BA.2.13, BA.3, BA.5, BQ.1.1, and XBB. Vaccination in mice using monovalent vaccines elicited the highest neutralizing titers against each self-matched strain, but against other variants were reduced 2− to 73-fold. A bivalent vaccine consisting of WT and BA.5 broadened the neutralizing breadth against pre-Omicron and Omicron subvariants except XBB. Among bivalent vaccines based on the strains before the emergence of XBB, a bivalent vaccine consisting of BA.2 and BA.5 elicited the most potent neutralizing antibodies against Omicron subvariants, including XBB. In mice primed with injected WT vaccine, intranasal booster with a bivalent vaccine containing XBB and BA.5 could elicit broad serum and respiratory mucosal neutralizing antibodies against all late Omicron subvariants, including XBB. In mice that had been sequentially vaccinated with WT and BA.5, intranasal booster with a monovalent XBB vaccine elicited greater serum and mucosal XBB neutralizing antibodies than bivalent vaccines containing XBB. Both monovalent and bivalent XBB vaccines induced neutralizing antibodies against EG.5. Unlike the antibody response, which is highly variant-specific, mice receiving either monovalent or bivalent vaccines elicited comparable T-cell responses against all variants. Furthermore, intranasal but not intramuscular booster induced antigen-specific lung resident T cells. This study provides insights into the design of the COVID-19 vaccine and vaccination strategies.

We study an avascular spherical solid tumour model with cell physiological age and resource constraints in vivo. We divide the tumour cells into three components: proliferating cells, quiescent cells and dead cells in necrotic core. We assume that the division rate of proliferating cells is nonlinear due to the nutritional and spatial constraints. The proportion of newborn tumour cells entering directly into quiescent state is considered, since this proportion can respond to the therapeutic effect of drug. We establish a nonlinear age-structured tumour cell population model. We investigate the existence and uniqueness of the model solution and explore the local and global stabilities of the tumour-free steady state. The existence and local stability of the tumour steady state are studied. Finally, some numerical simulations are performed to verify the theoretical results and to investigate the effects of different parameters on the model.

Abstract Wound infections pose a major healthcare issue, affecting the well‐being of millions of patients worldwide. Effective intervention and on‐site detection are important in wound management. However, current approaches are hindered by time‐consuming analysis and a lack of technology for real‐time monitoring and prompt therapy delivery. In this study, a smart wound patch system (SWPS) designed for wireless closed‐loop and in‐situ wound management is presented. The SWPS integrates a microfluidic structure, an organic electrochemical transistor (OECT) based sensor, an electrical stimulation module, and a miniaturized flexible printed circuit board (FPCB). The OECT incorporates a bacteria‐responsive DNA hydrogel‐coated gate for continuous monitoring of bacterial virulence at wound sites. Real‐time detection of OECT readings and on‐demand delivery of electrical cues to accelerate wound healing is facilitated by a mobile phone application linked with an FPCB containing low‐power electronics equipped with parallel sensing and stimulation circuitry. In this proof‐of‐concept study, the functionality of the SWPS is validated and its application both in vitro and in vivo is demonstrated. This proposed system expands the arsenal of tools available for effective wound management and enables personalized treatment.

Enzyme-induced carbonate precipitation (EICP) is an emanating, eco-friendly and potentially sound technique that has presented promise in various geotechnical applications. However, the durability and microscopic characteristics of EICP-treated specimens against the impact of drying-wetting (D-W) cycles is under-explored yet. This study investigates the evolution of mechanical behavior and pore characteristics of EICP-treated sea sand subjected to D-W cycles. The uniaxial compressive strength (UCS) tests, synchrotron radiation micro-computed tomography (micro-CT), and three-dimensional (3D) reconstruction of CT images were performed to study the multiscale evolution characteristics of EICP-reinforced sea sand under the effect of D-W cycles. The potential correlations between microstructure characteristics and macro-mechanical property deterioration were investigated using gray relational analysis (GRA). Results showed that the UCS of EICP-treated specimens decreases by 63.7% after 15 D-W cycles. The proportion of mesopores gradually decreases whereas the proportion of macropores increases due to the exfoliated calcium carbonate with increasing number of D-W cycles. The microstructure in EICP-reinforced sea sand was gradually disintegrated, resulting in increasing pore size and development of pore shape from ellipsoidal to columnar and branched. The gray relational degree suggested that the weight loss rate and UCS deterioration were attributed to the development of branched pores with a size of 100–1000 μm under the action of D-W cycles. Overall, the results in this study provide a useful guidancee for the long-term stability and evolution characteristics of EICP-reinforced sea sand under D-W weathering conditions.

The insulin receptor (IR) plays a crucial role in the growth and metabolism of animals. However, there are still many questions regarding the IR in crustaceans, particularly their role in shrimp growth and glucose metabolism. In this study, we identified a novel insulin-like receptor gene in Litopenaeus vannamei and cloned its full length of 6439 bp. This gene exhibited a highly conserved sequence and structural characteristics. Phylogenetic analysis confirmed it as an unreported RTK2-type IR, namely, LvRTK2. Expression pattern analysis showed that LvRTK2 is primarily expressed in female reproductive and digestive organs. Through a series of in vivo and in vitro experiments, including glucose treatment, exogenous insulin treatment, and starvation treatment, LvRTK2 was confirmed to be involved in the endogenous glucose metabolic pathway of shrimp under different glucose variations. Moreover, long-term and short-term interference experiments with LvRTK2 revealed that the interference significantly reduced the shrimp growth rate and serum glucose clearance rate. Further studies indicated that LvRTK2 may regulate shrimp growth by modulating the downstream PI3K/AKT signaling pathway and a series of glucose metabolism events, such as glycolysis, gluconeogenesis, glycogen synthesis, and glycogenolysis. This report on the characteristics and functions of LvRTK2 confirms the important role of RTK2-type IRs in regulating shrimp growth and glucose metabolism.

This study, grounded in psychological capital theory and the work resource model, investigates the impact of construction workers’ work resilience on their leisure sports participation. An interdisciplinary integration model was developed, incorporating work resilience, safety and health awareness, and leisure sports participation, providing empirical evidence for enhancing the well-being of the construction workforce. The study specifically examines the roles of physiological resilience, psychological resilience, social support, and occupational engagement in this context. A survey conducted with 663 construction workers in China and Korea revealed the following: (1) physiological resilience, psychological resilience, social support, and occupational engagement significantly and positively influence workers’ leisure sports participation; (2) safety and health awareness serves as a significant mediator between work resilience and leisure sports participation among construction workers. These findings underscore the importance of improving the work environment and creating semi-natural restorative landscapes to promote the physical and mental health of construction workers, as well as their engagement in leisure sports. The study calls for governmental and community initiatives, including policy support and resource allocation, to comprehensively enhance the overall well-being of construction workers.

Background: Recurrent aphthous ulcer (RAU) had high prevalence and lacked widely recognized treatment. Total glucosides of paeony (TGP) was used in the treatment of RAU in recent years. This study was to summarize the efficacy and safety of TGP in the treatment of RAU.Methods: We searched eight commonly used databases for relevant studies that published before 1 November 2023. Primary outcome was visual analogue scale (VAS). Secondary outcomes included overall response rate, significant response rate, ulcer healing time, interval, number of ulcers, and serum inflammatory factors. We conducted the meta-analysis, assessed risk of bias and the confidence of the evidence, by using Stata 15.0, Review Manager 5.4, and Gradepro.Results: Nine randomized controlled trials (RCTs) encompassing 883 patients with RAU were included in the final analysis. The VAS in the TGP group was lower than that in the control group (MD = −1.18, 95% CI = −1.58 to −0.78, p < 0.001, moderate-certainty evidence), subgroup analysis suggested longer (>8 weeks) medication and observation led to a more significant reduction in pain (p = 0.02). Moreover, TGP had higher overall response rate (RR = 1.18, 95% CI = 1.04 to 1.33, p = 0.008, very low-certainty evidence) and significant response rate (RR = 1.72, 95% CI = 1.38 to 2.14, p < 0.001, very low-certainty evidence), accelerated ulcer healing (MD = −1.79, 95% CI = −2.67 to −0.91, p < 0.001, low-certainty evidence), and extended intervals (MD = 23.60, 95% CI = 14.17 to 33.03, p < 0.001, very low-certainty evidence). The efficacy of TGP in reducing the number of ulcers showed no significant difference compared to the control group (MD = −1.66, 95% CI = −3.60 to 0.28, p = 0.09, low-certainty evidence). Moreover, TGP treatment was associated with a higher incidence of abdominal symptoms (RR = 3.27, 95% CI = 1.62 to 6.60, p < 0.001).Conclusion: TGP appears to hold promise as a widely-used clinical therapeutic option for treating RAU. Nevertheless, further rigorous studies of high quality are required to validate its effectiveness.Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=471154, Identifier CRD42023471154

Smoking has gradually become a very common behavior, and the related situation in different groups also presents different forms. Due to the differences of individual smoking cessation time and the interference of environmental factors in the spread of smoking behavior, we establish a stochastic giving up smoking model with quit-smoking duration. We also consider the saturated incidence rate. The total population is composed of potential smokers, smokers, quitters and removed. By using Itô's formula and constructing appropriate Lyapunov functions, we first ensure the existence of a unique global positive solution of the stochastic model. In addition, a threshold condition for extinction and permanence of smoking behavior is deduced. If the intensity of white noise is small, and $ \widetilde{\mathcal{R}}_0 < 1 $, smokers will eventually become extinct. If $ \widetilde{\mathcal{R}}_0 > 1 $, smoking will last. Then, the sufficient condition for the existence of a unique stationary distribution of the smoking phenomenon is studied as $ R_0^s > 1 $. Finally, conclusions are explained by numerical simulations.

Balance is important in lifesaving competitions. We aimed to investigate the relationship between dynamic balance and physical characteristics and functions in elite lifesavers by measuring the foot muscle cross-sectional area, ankle joint muscle strength, toe grasp strength, plantar superficial sensation, and dynamic balance (Y-balance test). In this observational study, we measured the foot muscle cross-sectional area, ankle dorsiflexion, plantar flexion, external flexion, isometric muscle strength, toe grasp strength, and superficial foot sensation of 15 adult lifesavers (12 males). The results show that toe grasp strength and ankle plantar flexion isometric muscle strength are particularly important for the dynamic balance of elite lifesavers working on sandy surfaces. Sand training improves intrinsic muscle strength and dynamic balance function. However, high training intensity may reduce plantar surface sensation; this needs to be verified through sand training interventions in the future.

Dinoflagellates and heterotrophic bacteria are two major micro-organism groups within marine ecosystems. Their coexistence has led to a co-evolutionary relationship characterized by intricate interactions that not only alter their individual behaviors but also exert a significant influence on the broader biogeochemical cycles. Our review commenced with an analysis of bacterial populations, both free-living and adherent to dinoflagellate surfaces. Members of Alphaproteobacteria, Gammaproteobacteria, and the Cytophaga–Flavobacterium–Bacteroides group are repeatedly found to be associated with dinoflagellates, with representation by relatively few genera, such as Methylophaga, Marinobacter, and Alteromonas. These bacterial taxa engage with dinoflagellates in a limited capacity, involving nutrient exchange, the secretion of pathogenic substances, or participation in chemical production. Furthermore, the genomic evolution of dinoflagellates has been profoundly impacted by the horizontal gene transfer from bacteria. The integration of bacterial genes into dinoflagellates has been instrumental in defining their biological characteristics and nutritional strategies. This review aims to elucidate the nuanced interactions between dinoflagellates and their associated bacteria, offering a detailed perspective on their complex relationship.

Searching for natural products with anti-tumor activity is an important aspect of cancer research. Seaweed polysaccharides from brown seaweed have shown promising anti-tumor activity; however, their structure, composition, and biological activity vary considerably, depending on many factors. In this study, 16 polysaccharide fractions were extracted and purified from three large brown seaweed species (Sargassum horneri, Scytosiphon lomentaria, and Undaria pinnatifida). The chemical composition analysis revealed that the polysaccharide fractions have varying molecular weights ranging from 8.889 to 729.67 kDa, and sulfate contents ranging from 0.50% to 10.77%. Additionally, they exhibit different monosaccharide compositions and secondary structures. Subsequently, their anti-tumor activity was compared against five tumor cell lines (A549, B16, HeLa, HepG2, and SH-SY5Y). The results showed that different fractions exhibited distinct anti-tumor properties against tumor cells. Flow cytometry and cytoplasmic fluorescence staining (Hoechst/AO staining) further confirmed that these effective fractions significantly induce tumor cell apoptosis without cytotoxicity. qRT-RCR results demonstrated that the polysaccharide fractions up-regulated the expression of Caspase-3, Caspase-8, Caspase-9, and Bax while down-regulating the expression of Bcl-2 and CDK-2. This study comprehensively compared the anti-tumor activity of polysaccharide fractions from large brown seaweed, providing valuable insights into the potent combinations of brown seaweed polysaccharides as anti-tumor agents.

The active thermal management (ATM) method can improve the current capability of power devices safely by utilizing the thermal limit of the device. However, the existing methods are not suitable for power devices affected by random convection. This is because the randomness of convection will lead to the uncertainty of increased current capability, which is unacceptable in most engineering applications. To overcome the shortcomings of existing ATM methods, this paper proposed a novel ATM method for power devices under the influence of random convection. In the proposed method, the short-time current capability of the power device is maximized according to the thermal inertia of the device and maximum load current. The continuous current capability of the power device is determined by a maximum continuous current that satisfies the over-temperature risk constraint of the device. To accurately estimate the maximum continuous current, an uncertainty model is presented for the convective thermal resistance of the power device based on wavelet packet transform and Markov chain. A series of experimental studies are carried out by taking a power MOSFET as an example. The experimental results show that the proposed method can safely improve the output performance of the power device without causing random fluctuations in the current capability.

The frequent occurrence of extreme weather events (EWEs) in recent years has posed major hazards to urban transportation as well as socioeconomic impacts. A quantitative evaluation of the urban transportation resilience to minimize the impact caused by EWEs becomes critical to the rapid recovery of urban transportation after disasters. However, there is, generally, a lack of reliable data sources to monitor urban transportation performance under EWEs. This empirical study proposes a performance indicator (displacement) and quantitative method for evaluating the urban transportation performance under EWEs based on bus GPS trajectory datasets. Furthermore, the transportation resilience of it is quantified, and the variation is compared across temporal and spatial dimensions. The method is applied in a case study of Fuzhou, China, under rainstorm events. The results show that the Gulou and Jinan subareas have the highest transportation resilience during the yellow and red rainstorm warnings. By formulating an emergency plan and taking mitigation measures, the transportation performance in the Jinan subarea during the red rainstorm warning was improved by 36% compared to the yellow rainstorm warning. The empirical study not only fills the knowledge gap for quantifying the transportation resilience across the geographical boundary under rainstorm events, but also estimates the operation status of the road network. The results will help policymakers prioritize the resource distribution and develop effective policies or measures to further improve transportation resilience in the city.

Abstract As an important edge device of power grid, smart meters enable the detection of illegal behaviors such as electricity theft by analyzing large-scale electricity consumption data. Electricity theft poses a major threat to the economy and the security of society. Electricity theft detection (ETD) methods can effectively reduce losses and suppress illegal behaviors. On electricity consumption data from smart meters, ETD methods always train deep learning models. However, these methods are limited to extract different electricity consumption characteristics between independent users, and the pattern differences between users cannot be actively learned. Such difficulty prevents ETD further performance improvement. Therefore, a novel ETD method is proposed, which is the first attempt to apply supervised contrastive learning for electricity theft detection. On the one hand, our method allows the detection model to improve its detection performance by actively comparing users’ representation vectors. On the other hand, in order to obtain high-quality augmented views, largest triangle three buckets time series downsampling is adopted innovatively to improve model stability through data augment. Experiments on real-world datasets show that our model outperforms state-of-the-art models.

Abstract Objectives Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile of circRNA in lung adenocarcinoma (LUAD). Methods This study included 14 pairs of postoperative lung adenocarcinoma specimens, including cancer tissues and matched adjacent tissues. Second‐generation sequencing was applied to the specimens to determine the circRNA expression in them among the 5242 distinct circRNAs detected. Results We identified a total of 18 significantly dysregulated circRNAs in the LUAD tissues: upregulation in four and downregulation in 14. ROC (The receiver operating characteristic curve) further suggested that hsa_circ_0120106, has_circ_0007342, has_circ_0005937, and circRNA_0000826 could potentially be used as biomarkers in the diagnosis of LUAD. Furthermore, study of the circRNA–microRNA (miRNA)–messenger RNA (mRNA) revealed interactions between the 18 dysregulated circRNA and several cancer‐related miRNAs. Finally, a further Kyoto Encyclopedia of Genes and Genomes analysis showed that the cell cycle phase transition, p53 signaling pathway, AMP‐activated protein kinase (AMPK) relative signaling pathway, and so on were key putative pathways in the process of LUAD. Conclusions These findings demonstrated the correlation between abnormality in circRNA expression and LUAD, which lays the foundation of making CircRNAs candidate biomarkers in the diagnosis of LUAD.

Fibula transplantation plays an irreplaceable role in restoring the function and morphology of the defected mandible. However, the complex load-bearing environment of the mandible makes it urgent to accurately reconstruct the mandible, ensure the position of the condyle after surgery, and restore the patient’s occlusal function and contour. The intervention of digital design and three-dimensional (3D) printed titanium mesh provides a more efficient method and idea to solve this problem. Digital design guides the accurate positioning, osteotomy, and simultaneous implant placement during surgery, and 3D printed titanium mesh ensures stable condyle position after surgery, restoring good mandibular function. The double-layer folded fibula maintains the vertical height of the mandible and a good facial contour, and simultaneous implant placement can establish a good occlusal relationship. This study conducted a retrospective analysis of five patients with jaw defects who underwent digital fibula reconstruction over the past 3 years. It was found that the surgical protocol combining digital design, 3D printed intraoperative guides, 3D printed titanium mesh, free fibula flap, immediate implant, and occlusal reconstruction to repair jaw defects had more ideal facial appearance and biological function. It will provide a more reliable surgical protocol for clinical management of large mandibular defects.

A stochastic mathematical model is proposed to study how environmental heterogeneity and the augmentation of mosquitoes with [Formula: see text] bacteria affect the outcomes of dengue disease. The existence and uniqueness of the positive solutions of the system are studied. Then the V-geometrically ergodicity and stochastic ultimate boundedness are investigated. Further, threshold conditions for successful population replacement are derived and the existence of a unique ergodic steady-state distribution of the system is explored. The results show that the ratio of infected to uninfected mosquitoes has a great influence on population replacement. Moreover, environmental noise plays a significant role in control of dengue fever.

ABSTRACTProlonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but not in macaques with low viral loads (< 105, SIVlow). After a second vaccination, the immune responses are robustly enhanced in all uninfected and SIVlow macaques. These responses also show a moderate increase in 70% SIVhi macaques but decline sharply soon after. Further analysis reveals that decreased antibody and CMI responses are associated with reduced circulating follicular helper T cell (TFH) counts and aberrant CD4/CD8 ratios, respectively, indicating that dysregulation of CD4+ T cells by SIV infection impairs the COVID-19 vaccine-induced immunity. Ad5-vectored COVID-19 vaccine shows no impact on SIV loads or SIV-specific CMI responses. Our study underscores the necessity of frequent booster vaccinations in HIV-infected patients and provides indicative biomarkers for predicting vaccination effectiveness in these patients.

Abstract The highly contagious SARS-CoV-2 Omicron subvariants severely attenuated the effectiveness of currently licensed SARS-CoV-2 vaccines based on ancestral strains administered via intramuscular injection. In this study, we generated a recombinant, replication-incompetent human adenovirus type 5, Ad5-S-Omicron, that expresses Omicron BA.1 spike. Intranasal, but not intramuscular vaccination, elicited spike-specific respiratory mucosal IgA and residential T cell immune responses, in addition to systemic neutralizing antibodies and T cell immune responses against most Omicron subvariants. We tested intranasal Ad5-S-Omicron as a heterologous booster in mice that previously received intramuscular injection of inactivated ancestral vaccine. In addition to inducing serum broadly neutralizing antibodies, there was a significant induction of respiratory mucosal IgA and neutralizing activities against Omicron subvariants BA.1, BA.2, BA.5, BA.2.75, BF.7 as well as pre-Omicron strains Wildtype, Beta, and Delta. Serum and mucosal neutralizing activities against recently emerged XBB, BQ.1, and BQ.1.1 could also be detected but were much lower. Nasal lavage fluids from intranasal vaccination contained multimeric IgA that can bind to at least 10 spike proteins, including Omicron subvariants and pre-Omicron strains, and possessed broadly neutralizing activities. Intranasal vaccination using Ad5-S-Omicron or instillation of intranasal vaccinee’s nasal lavage fluids in mouse nostrils protected mice against Omicron challenge. Taken together, intranasal Ad5-S-Omicron booster on the basis of ancestral vaccines can establish effective mucosal and systemic immunity against Omicron subvariants and multiple SARS-CoV-2 variants. This candidate vaccine warrants further development as a safe, effective, and user-friendly infection and transmission-blocking vaccine.

ABSTRACT Latent viral reservoir is recognized as the major obstacle to achieving a functional cure for HIV infection. We previously reported that arsenic trioxide (As2O3) combined with antiretroviral therapy (ART) can reactivate the viral reservoir and delay viral rebound after ART interruption in chronically simian immunodeficiency virus (SIV)-infected macaques. In this study, we further investigated the effect of As2O3 independent of ART in chronically SIV-infected macaques. We found that As2O3-only treatment significantly increased the CD4/CD8 ratio, improved SIV-specific T cell responses, and reactivated viral latency in chronically SIVmac239-infected macaques. RNA-sequencing analysis revealed that As2O3 treatment downregulated the expression levels of genes related to HIV entry and infection, while the expression levels of genes related to transcription initiation, cell apoptosis, and host restriction factors were significantly upregulated. Importantly, we found that As2O3 treatment specifically induced apoptosis of SIV-infected CD4+ T cells. These findings revealed that As2O3 might not only impact viral latency, but also induce the apoptosis of HIV-infected cells and thus block the secondary infection of bystanders. Moreover, we investigated the therapeutic potential of this regimen in acutely SIVmac239-infected macaques and found that As2O3 + ART treatment effectively restored the CD4+ T cell count, delayed disease progression, and improved survival in acutely SIV-infected macaques. In sum, this work provides new insights to develop As2O3 as a component of the “shock-and-kill” strategy toward HIV functional cure. IMPORTANCE Although antiretroviral therapy (ART) can effectively suppress the viral load of AIDS patients, it cannot functionally cure HIV infection due to the existence of HIV reservoir. Strategies toward HIV functional cure are still highly anticipated to ultimately end the pandemic of AIDS. Herein, we investigated the direct role of As2O3 independent of ART in chronically SIV-infected macaques and explored the underlying mechanisms of the potential of As2O3 in the treatment of HIV/SIV infection. Meanwhile, we investigated the therapeutic effects of ART+As2O3 in acutely SIVmac239-infected macaques. This study showed that As2O3 has the potential to be launched into the “shock-and-kill” strategy to suppress HIV/SIV reservoir due to its latency-reversing and apoptosis-inducing properties.

In this paper, a method of generating true random numbers obeying multiple distribution characteristics is proposed. First, two resistance-capacitance (RC) self-excited oscillation circuits are used to generate two jittered, periodically unstable square wave signals, and then a text high-precision and high-frequency quartz crystal oscillator is used to sample and measure these two jittered signals to obtain their periods. Due to the randomness of the external environment, there is Gaussian white noise in the circuit, so the periods of these two signals are random variables subject to the Gaussian distribution. Finally, we use a text field-programmable gate array (FPGA) to perform secondary processing on these two random signals, which can generate true random number sequences that conform to the distribution characteristics of Gaussian distribution, Poisson distribution, 0–1 distribution, uniform distribution, etc. The random numbers generated by the circuit can be applied to the field of intelligent control related to automatic control and machine learning. This method uses a physical entropy source to generate high-quality true random sequences, which are easy to build, low in circuit cost, and small in size. The experimental results show that the method is low-cost, highly reliable and easy to integrate, providing an effective solution for the generation of true random numbers in electronic systems.

In order to analyze the propagation path of vibration noise, and carry out finite element numerical calculation research based on electro-magnetic–solid-acoustic multiphysics coupling, a single-phase two-column dry-type transformer is used as the noise source in this paper. According to the distribution characteristics of the spatial sound field and the vibration noise amplitude–frequency characteristics of the transformer, a topology design method of the space coiled acoustic metamaterial (SCAMM) unit is proposed and the analytical calculation methods of acoustic parameters such as sound pressure reflection/transmission coefficient of various SCAMM units are deduced. Besides, the effects of parameters such as the longitudinal width a, the waveguide width b, the ratio of waveguide width to longitudinal width b/aand transverse width t on the SCAMM unit sound transmission loss (STL) are studied, and the anti-resonance frequencies are adjusted by parameter optimization method to achieve the optimal sound insulation effect. In the end, the sound field control barrier based on the SCAMM for transformer vibration and noise suppression is designed, and relevant experiments are carried out. The experiments show that the sound field control barrier based on the SCAMM can reduce the transformer noise sound pressure level (SPL) of the main noise frequencies by up to 25 dB with a thickness of only 4 cm and ventilation.

Introduction: There has been a lack of treatments available to lower the frequency of recurrent aphthous ulcers (RAUs) until now. Total glucosides of paeony (TGP) is a botanical drug extracted from the dried roots of Paeonia lactiflora Pall. [Ranunculaceae; Paeoniae Radix Alba]. This study aims to evaluate the efficacy and safety of TGP in the treatment of RAU.Methods: This study was registered with the Chinese Clinical Trial Registry (ChiCTR1900025623). Patients were randomly assigned to the TGP or placebo group and treated with 1.8 g/day for 24 weeks. Participants were observed for a total of 36 weeks and were asked to record ulcer severity, medication, and adverse reactions in the form of diaries or apps every day. The primary outcome was the monthly ulcer-free interval.Results: A total of 79 individuals were enrolled, with 40 assigned to the TGP group and 39 to the placebo group. The dropout rate was 18.18%. In the TGP group, the monthly ulcer-free interval was significantly longer than baseline (median, 9.6 days) since weeks 13–24 (median, 18.5 days) (p < 0.05), and after discontinuation, it was further prolonged (median, 24.7 days) than in weeks 13–24 (p < 0.05). In addition, the monthly ulcer-free interval was longer in the TGP group than in the placebo group (median, 15.9 days) at weeks 25—36 (p < 0.001). There were better improvements in the monthly number of ulcers and monthly area of ulcers, and visual analog scoring in the TGP group at weeks 25—36 (p < 0.001).Conclusion: TGP had a good long-term therapeutic effect on RAU with frequent occurrence.Systematic Review Registration:www.chictr.org.cn, identifier ChiCTR1900025623.

The main purpose of this cross-sectional study was to examine the impact of the inter-limb asymmetry of hamstring strength on jump, sprint and strength performance and to compare the effects of inter-limb asymmetry of hamstring strength with gross force (GF) of the hamstring on these physical qualities in youth volleyball athletes. Eighty-one youth volleyball players (age: 16.6 ± 1.9 years; training experience: 3.0 ± 0.9 years; height: 191.4 ± 7.1 cm; body mass: 78.5 ± 12.9 kg; lean body mass: 63.5 ± 10.5 kg; body fat rate: 18.6% ± 6.1%) performed a mid-season battery of tests consisting of morphological test, depth jump (DJ), counter movement jump (CMJ), squat jump (SJ), 10 m sprint, isometric mid-thigh pull (IMTP) and hamstring strength test. All tests reported good to excellent reliability (ICC range = 0.815–0.996) and acceptable variability (CV range = 3.26–7.84%). Results show a significant negative relationship between inter-limb asymmetry of hamstring strength and all physical qualities (r = −0.271 to −0.445; p < 0.05), and a significant positive relationship between GF of hamstring and all physical qualities (r = 0.303 to 0.664; p < 0.05). Additionally, GF of hamstring was more relevant to IMTP-PF (peak force) (r = 0.664) and inter-limb asymmetry of hamstring strength was more relevant to 10 m sprint (r = −0.445). The findings from this study indicate that, for youth athletes, the GF of the hamstring is crucial for overall lower limb strength performance, and the importance of inter-limb symmetry of hamstring strength increases with the complexity of the task.

Thermochromic materials can adjust their formal reflectivity in response to changes in ambient temperature, so they offer a means of achieving seasonal thermal control and have been a reasonable choice to enhance the solar radiation heat in winter and lower the solar radiation heat in summer. To optimize thermochromic coatings to meet the application requirement on architectural surface color, the effect of the material color was studied on optical properties of thermochromic coatings under two transition temperatures of 25 °C and 33 °C. A measurement system was designed to analyze the reflectivity spectra and solar reflectivity of thermochromic coatings in both colored and colorless phases. Experimental results show that all samples exhibited a significant increase in reflectivity from the colored phase to the colorless phase especially in the 1300 nm–1600 nm and 1900 nm–2100 nm wavelengths. The global reflectivity of the thermochromic coatings could be increased by 3.17%–10.68% under 25 °C and 7.22%–18.23% under 33 °C. From the view of evaluating the optical properties, the purple-blue and dark-green colors are respectively the best choices for thermochromic coatings. The experimental data proposed the efficient reference for application of thermochromic materials in buildings.

The tribological behavior of 42CrMo4/17NiCrMo6-4 under grease lubrication was explored in terms of load, speed, hardness matching, and lubrication quantity. Optical microscopy, scanning electron microscopy, and a surface profilometer were used to investigate the wear mechanism. The results show that hardness matching has the greatest impact on the wear resistance and friction reduction of the friction pair, followed by the load factor, with the impacts of speed and lubricant quantity being minor. Increasing the hardness of 42CrMo4 reduces the friction coefficient and wear volume of the friction pair substantially. When the maximum surface hardness of 42CrMo4 was compared with the lowest surface hardness, the friction coefficient was reduced by 21.5%, and the wear volume was reduced by 87.2%. Abrasive wear is the sort of wear failure that was seen, and as the hardness of 42CrMo4 increased, more severe fatigue wear appeared on 17NiCrMo6-4. While the wear volume initially increases and subsequently lowers with increasing load, the friction coefficient initially decreases and then stabilizes. A synergistic combination of abrasive and adhesive wear occurs under high load, changing the wear type from abrasive wear under low load. The wear volume is decreased by the sticky layer generated under high load conditions, which achieves superior wear prevention. This study is anticipated to offer recommendations for designing gears’ required hardness under various operating circumstances.

Abstract Background Floating toes are a condition and deformity in which some of the toes are afloat. Many functional impairments in floating toes have been previously studied lately and several factors related to floating toes have also been reported. However, no reports have considered the relationship between lifestyle and floating toes among children. The purpose of this study was to reveal the prevalence of floating toes among school children and reveal its relationship with lifestyle. Methods In total, 138 young male baseball players were recruited. Lifestyle was evaluated by using a questionnaire and chosen whether the main lifestyle was Japanese or Western, if the bedding was futons or beds, and if the toilet was Japanese style (a squat toilet) or Western style. Floating toes were defined as toes that were not in contact with the mat. Ankle dorsiflexion in the knee‐flexed and knee‐extended positions was measured in a weight‐bearing position. The relationship between the floating toes and lifestyles, and the comparison of ankle dorsiflexion range of motion between the lifestyles were statistically analyzed. Results Players living in a Western style showed a significantly higher prevalence of floating toes on both feet compared with the players living in a Japanese style (throwing side; 39% vs. 19%, p = 0.04, and non‐throwing side; 43% vs. 19%, p = 0.01). Players living in a Western style with beds showed a significantly smaller range of motion on both sides of ankle dorsiflexion in the knee‐flexed position compared with those who were not (throwing side; 37.2 ± 5.7° vs. 39.0 ± 6.6°, p = 0.04, and non‐throwing side; 36.8 ± 5.8° vs. 38.6 ± 6.1°, p = 0.04). Conclusion Children mainly living in a Western lifestyle showed a significantly higher prevalence of floating toes on both feet compared to those mainly living in a Japanese lifestyle. The prevalence of floating toes may be related to lifestyles among children. Trial registration The study was approved by the institutional review board of the Waseda University Graduate School of Sport Sciences (IRB number 2021–185).

BackgroundA third mRNA vaccine booster is recommended to improve immunity against SARS-CoV-2 in kidney transplant recipients (KTRs). However, the immunity against SARS-CoV-2 Ancestral strain and Delta and Omicron variants elicited by the third dose of inactivated booster vaccine in KTRs remains unknown.MethodsThe blood parameters related to blood cells count, hepatic function, kidney function, heart injury and immunity were explored clinically from laboratory examinations. SARS-CoV-2 specific antibody IgG titer was detected using an enzyme-linked immunosorbent assay. Cellular immunity was analyzed using interferon-γ enzyme-linked immunospot assay.ResultsThe results showed that there were no severe adverse effects and apparent changes of clinical laboratory biomarkers in KTRs and healthy volunteers (HVs) after homologous inactivated vaccine booster. A third dose of inactivated vaccine booster significantly increased anti-Ancestral-spike-trimer-IgG and anti-Ancestral-receptor binding domain (RBD)-IgG titers in KTRs and HVs compared with the second vaccination. However, the anti-Delta-RBD-IgG and anti-Omicron-RBD-IgG titers were significantly lower than anti-Ancestral-RBD-IgG titer in KTRs and HVs after the third dose. Notably, only 25.6% (10/39) and 10.3% (4/39) of KTRs had seropositivity for anti-Delta-RBD-IgG and anti-Omicron-RBD-IgG after booster, which were significantly lower than HVs (anti-Delta-RBD-IgG: 100%, anti-Omicron-RBD-IgG: 77.8%). Ancestral strain nucleocapsid protein and spike specific T cell frequency after booster was not significantly increased in KTRs compared with the second dose, significantly lower than that in HVs. Moreover, 33.3% (12/36), 14.3% (3/21) and 14.3% (3/21) of KTRs were positive for the Ancestral strain and Delta and Omicron spike-specific T cells, which were significantly lower than HVs (Ancestral: 80.8%, Delta: 53.8%, and Omicron: 57.7%).ConclusionsA third dose of inactivated booster vaccine may significantly increase humoral immunity against the Ancestral strain in KTRs, while humoral and cellular immunity against the Delta and Omicron variants were still poor in KTRs.

This paper presents a comparative analysis of thermal and mechanical behaviors of the thermally stratified heat storage system using three different heat transfer materials, which are the binary nitrate salt, ternary nitrate salt and liquid lead–bismuth eutectic (LBE) alloy. The discharging behaviors of the tank using three different heat transfer materials are investigated by using both the algebraic and numerical models. For all the three materials, the algebraic results are consistent with the simulation ones. During the discharging process, in comparison, the thermal stratification thickness of the tank using the binary nitrate salt is relatively smaller, which can lead to a better discharging performance. The comparison results of expanding behaviors of the standby tank reveal that the thermal stratification thicknesses of tanks using the three different heat transfer materials all increase with the standby time increased. Compared with the other two materials, the thermal stratification thickness of the tank using the binary nitrate salt is the smallest at the same standby time. The comparison results of mechanical behaviors of the tank demonstrate that the maximum mechanical stress of the tank using the liquid LBE is the greatest, while the tank using the molten salts can have better mechanical properties.

Abstract Background To develop a risk model based on dosimetric metrics to predict local recurrence in nasopharyngeal carcinoma (NPC) patients treated with intensive modulated radiation therapy (IMRT). Methods 493 consecutive patients were included, among whom 44 were with local recurrence. One-to-two propensity score matching (PSM) was used to balance variables between recurrent and non-recurrent groups. Dosimetric metrics were extracted, and critical dosimetric predictors of local recurrence were identified by Cox regression model. Moreover, recurrent sites and patterns were examined by transferring the recurrent tumor to the pretreatment planning computed tomography. Results After PSM, 44 recurrent and 88 non-recurrent patients were used for dosimetric analysis. The univariate analysis showed that eight dosimetric metrics and homogeneity index were significantly associated with local recurrence. The risk model integrating D 5 and D 95 achieved a C-index of 0.706 for predicting 3-year local recurrence free survival (LRFS). By grouping patients using median value of risk score, patients with risk score ˃ 0.885 had significantly lower 3-year LRFS (66.2% vs. 86.4%, p = 0.023). As for recurrent features, the proportion of relapse in nasopharynx cavity, clivus, and pterygopalatine fossa was 61.4%, 52.3%, and 40.9%, respectively; and in field, marginal, and outside field recurrence constituted 68.2%, 20.5% and 11.3% of total recurrence, respectively. Conclusions The current study developed a novel risk model that could effectively predict the LRFS in NPC patients. Additionally, nasopharynx cavity, clivus, and pterygopalatine fossa were common recurrent sites and in field recurrence remained the major failure pattern of NPC in the IMRT era.

Background: A new form of cell death, copper-dependent cell death (termed cuproptosis), was illustrated in a recent scientific study. However, the biological function or prognostic value of cuproptosis regulators in bladder cancer (BLCA) remains unknown.Materials and Methods: Sequencing data obtained from BLCA samples in TCGA and GEO databases were preprocessed for analysis. Biological function and immune cell infiltration levels evaluated by gene set variation analysis (GSVA) were employed to calculate enrichment scores. Iteration least absolute shrinkage and selection operator (LASSO) and COX regression model were employed to select feature genes and construct a novel cuproptosis-related (CR) score signature. The genomics of drug sensitivity in cancer (GDSC) and tumor immune dysfunction and exclusion (TIDE) analysis were used to predict the chemotherapy and immunotherapy efficacy for BLCA patients. The relative expression of the genes involved in the signature was also verified by real-time quantitative PCR (qRT-PCR) in cell lines and tissues.Results: Expression abundance and the prognostic value of cuproptosis regulators proved that cuproptosis might play a vital part in the carcinogenesis of BLCA. GSVA revealed that cuproptosis regulators might be associated with metabolism and metastasis-related pathways such as TGF-β, protein secretion, oxidative Phosphorylation, MYC targets, MTORC1, and adipogenesis pathways. CR scores could predict the prognosis and evaluate the chemotherapy and immunotherapy efficacies of BLCA. CR scores were positively correlated with EMT, MYC, MTORC1, HEDGEHOG, and E2F signaling pathways; meanwhile, they were negatively correlated with several immune cell infiltration levels such as CD8+ T cells, γδT cells, and activated dendritic cells. Several GEO datasets were used to validate the power of prognostic prediction, and a nomogram was also established for clinical use. The expressions of DDX10, RBM34, and RPL17 were significantly higher in BLCA cell lines and tissues in comparison with those in the corresponding normal controls.Conclusion: Cuproptosis might play an essential role in the progression of BLCA. CR scores could be helpful in the investigation of prognostic prediction and therapeutic efficacy and could make contributions to further studies in BLCA.

Molecular targeted therapy has shown potential in hepatocellular carcinoma (HCC) patients, and immunotherapy applications are developing rapidly. However, clinical guidance for making individualized therapy decisions for HCC patients remains lacking. MDH (Medication Decision in HCC) gene signatures comprising 70 genes were screened using transcriptomic data from multikinase inhibitor (TKI)-resistant HCC cells and HCC patient-derived xenograft model (PDX) models. Four MDH subtypes with distinct biological and clinical characteristics were defined by unsupervised cluster analysis of HCC data from The Cancer Genome Atlas (TCGA) database. To facilitate individualized and reasonable clinical guidance for each HCC patient, we constructed the MDH score. Comprehensive analysis suggested high MDH scores were associated with TKI resistance, a high proportion of stromal cell infiltration and poor survival outcomes. We recommend concomitant stromal activity intervention and immunotherapy for this type of HCC. Moreover, low MDH scores indicate TKI sensitivity, and a combination of targeted and immunotherapy is recommended. The nomogram constructed by iteration least absolute shrinkage and selection operator (LASSO) Cox regression analysis successfully predicted 3- or 5-year survival outcomes and mortality risks of HCC patients. In conclusion, TKI resistance model-based MDH gene signatures provide novel insight into potential mechanisms of drug resistance and heterogeneity in HCC. Integrative analysis plus a simplified decision model may aid personalized treatment and prognostic assessment among HCC patients.

Background: The role of the tumor microenvironment (TME) in predicting prognosis and therapeutic efficacy has been demonstrated. Nonetheless, no systematic studies have focused on TME patterns or their function in the effectiveness of immunotherapy in triple-negative breast cancer.Methods: We comprehensively estimated the TME infiltration patterns of 491 TNBC patients from four independent cohorts, and three cohorts that received immunotherapy were used for validation. The TME subtypes were comprehensively evaluated based on immune cell infiltration levels in TNBC, and the TRG score was identified and systematically correlated with representative tumor characteristics. We sequenced 80 TNBC samples as an external validation cohort to make our conclusions more convincing.Results: Two TME subtypes were identified and were highly correlated with immune cell infiltration levels and immune-related pathways. More representative TME-related gene (TRG) scores calculated by machine learning could reflect the fundamental characteristics of TME subtypes and predict the efficacy of immunotherapy and the prognosis of TNBC patients. A low TRG score, characterized by activation of immunity and ferroptosis, indicated an activated TME phenotype and better prognosis. A low TRG score showed a better response to immunotherapy in TNBC by TIDE (Tumor Immune Dysfunction and Exclusion) analysis and sensitivity to multiple drugs in GDSC (Genomics of Drug Sensitivity in Cancer) analysis and a significant therapeutic advantage in patients in the three immunotherapy cohorts.Conclusion: TME subtypes played an essential role in assessing the diversity and complexity of the TME in TNBC. The TRG score could be used to evaluate the TME of an individual tumor to enhance our understanding of the TME and guide more effective immunotherapy strategies.

The role of ferroptosis in tumor progression and metastasis has been demonstrated. Nonetheless, potential biological function of ferroptosis regulatory pattern in nasopharyngeal carcinoma (NPC) remains unknown. Ferroptosis regulatory patterns of nasopharyngeal carcinoma samples were evaluated based on 113 ferroptosis regulators and three distinct ferroptosis subtypes were determined by unsupervised clustering. The ferroptosis score (FEP score) was identified to quantify ferroptosis patterns within individual tumors by Gaussian finite mixture model and systematically correlated with representative tumor characteristics. Subtype 1 and subtype 3 were consistent with immune activated phenotype, while subtype 2 was consistent with immune suppressed phenotype. High ferroptosis score, characterized by immune activation and suppression of mRNA based stemness index (mRNAsi) and Epstein-Barr virus (EBV) genes, indicated an immune activated tumor microenvironment (TME) phenotype, with better progression free survival (PFS) and lower risk of recurrence and metastasis. Low ferroptosis score, characterized by activation of Wnt and NF-κB signaling pathways and lack of effective immune infiltration, indicated an immune suppressed tumor microenvironment phenotype and poorer survival. High ferroptosis score was also correlated to enhanced response to immunotherapy, and was confirmed to correlate with therapeutic advantages and clinical benefits in an anti-programmed cell death 1 ligand 1 (PD-L1) immunotherapy cohort. As ferroptosis played a crucial role in the tumor microenvironment’s diversity, assessing the ferroptosis pattern within individual tumor with ferroptosis score could enhance our understanding of tumor microenvironment infiltration characterization and help develop more effective immunotherapy.

A shortwave infrared Ge–Si photodetector will become the core device of the LiDAR optical receiver. In order to meet the urgent demand for photodetectors in the LiDAR field, we designed and produced a 32 × 32-pixel Ge–Si photodetector array, which was proposed and developed to meet the performance requirements of the detector array. A dark current detection system for fast scanning and detecting large-scale Ge–Si detector arrays was proposed and developed to achieve the rapid detection of dark current in each detector pixel, with a detection accuracy of less than 1 nA. The system validated the primary performance indicators of the detector array we designed, achieving rapid discrimination of the array performance and rapid localization of damaged pixels. The scanning test results showed that the average dark current of the designed detector array chip was on the nanoampere level, and the proportion of bad points was less than 1%. The consistency of the array chip was high, which meets the requirements of light detection at the receiving end of LiDAR. This work laid the foundation for our subsequent development of a LiDAR prototype system.

Polysaccharides from Grifola frondosa (G. frondosa) have anti-obesity and anti-inflammatory activities. In this study, the major type, molecular weight, homogeneity and structure of a polysaccharide purified from G. frondosa (denoted GFPA) were determined. In high-fat diet (HFD)-treated mice, 8 weeks of GFPA administration efficiently decreased body weight and blood glucose concentration and counteracted hyperlipidemia. GFPA efficiently decreased adipocyte size and ameliorated inflammatory infiltration in the three types of white adipose tissue and alleviated steatosis, fat accumulation and inflammatory infiltration in the livers of HFD-fed mice. GFPA also decreased the concentrations of aspartate aminotransferase, alanine aminotransferase and pro-inflammatory factors in the sera and livers of HFD-treated mice. Furthermore, GFPA was found to regulate lipid metabolism via the inhibition of ceramide levels in HFD-treated mice. GFPA exhibited strong anti-obesity effects via the modulation of chronic inflammation through Toll-like receptor 4/nuclear factor kappa-B signaling, which supports the use of GFPA for the treatment of obesity.

The decision-making control of autonomous driving in complex urban road environments is a difficult problem in the research of autonomous driving. In order to solve the problem of high dimensional state space and sparse reward in autonomous driving decision control in this environment, this paper proposed a Coordinated Convolution Multi-Reward Proximal Policy Optimization (CCMR-PPO). This method reduces the dimension of the bird’s-eye view data through the coordinated convolution network and then fuses the processed data with the vehicle state data as the input of the algorithm to optimize the state space. The control commands acc (acc represents throttle and brake) and steer of the vehicle are used as the output of the algorithm.. Comprehensively considering the lateral error, safety distance, speed, and other factors of the vehicle, a multi-objective reward mechanism was designed to alleviate the sparse reward. Experiments on the CARLA simulation platform show that the proposed method can effectively increase the performance: compared with the PPO algorithm, the line crossed times are reduced by 24 %, and the number of tasks completed is increased by 54 %.

Abstract In this paper, an impulsive quaternion-valued neural networks (QVNNs) model with leakage, discrete, and distributed delays is considered. Based on the homeomorphic mapping method, Lyapunov stability theorem, and linear matrix inequality (LMI) approach, sufficient conditions for the existence, uniqueness, and global robust stability of the equilibrium point of the impulsive QVNNs are provided. A numerical example is provided to confirm the obtained results. A conclusion is presented in the end.