14 results on '"Ziglari T"'
Search Results
2. Cancer stem cell-derived extracellular vesicles preferentially target MHC-II– macrophages and PD1+ T cells in the tumor microenvironment
- Author
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Gonzalez-Callejo, P., primary, Guo, Z., additional, Ziglari, T., additional, Claudio, N.M., additional, Oshimori, N., additional, Seras-Franzoso, J., additional, and Pucci, F., additional
- Published
- 2022
- Full Text
- View/download PDF
3. Outbreak of severe disseminated aspergillosis in a flock of ostrich (Struthio camelus)
- Author
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Khosravi, A. R., Shokri, H., Ziglari, T., Naeini, A. R., Mousavi, Z., and Hashemi, H.
- Published
- 2008
4. Assessment of growth-inhibiting effect of some plant essential oils on different Fusarium isolates
- Author
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Naeini, A., primary, Ziglari, T., additional, Shokri, H., additional, and Khosravi, A.R., additional
- Published
- 2010
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5. Evaluation of the IgY production against Hsp90 of Candida albicans
- Author
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Ziglari, T., primary, Khosravi, A.R., additional, Tadjbdkhsh, H., additional, Asadi, F., additional, Modirsaneii, M., additional, Shokri, H., additional, Nikbakhtborojeni, G.R., additional, and Rostamibeshman, M., additional
- Published
- 2009
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6. Candida species isolated from nails and their in vitro susceptibility to antifungal drugs in the department of Dermatology (University of Tehran, Iran)
- Author
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Khosravi, A.R., primary, Shokri, H., additional, Mansouri, P., additional, Katiraee, F., additional, and Ziglari, T., additional
- Published
- 2008
- Full Text
- View/download PDF
7. A study of mycoflora of the external ear canals in dromedary Camels in Iran
- Author
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Ali Reza Khosravi, Shokri, H., Ziglari, T., and Niasari-Naslaji, A.
8. Cancer stem cell-derived extracellular vesicles preferentially target MHC-II–macrophages and PD1+ T cells in the tumor microenvironment
- Author
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Patricia Gonzalez-Callejo, Zihan Guo, Tahereh Ziglari, Natalie Marcia Claudio, Kayla Hoang Nguyen, Naoki Oshimori, Joaquim Seras-Franzoso, Ferdinando Pucci, Institut Català de la Salut, [Gonzalez-Callejo P] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Bionanoplasmonics Group, CIC biomaGUNE, Donostia-San Sebastián, Spain. [Guo Z, Nguyen KH] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Program in Biomedical Sciences, Oregon Health and Science University, Portland, Oregon, United States of America. [Ziglari T] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. [Claudio NM] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Otolaryngology-Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America. [Oshimori N] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Program in Biomedical Sciences, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Otolaryngology-Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Dermatology, Oregon Health and Science University, Portland, Oregon, United States of America. [Seras-Franzoso J] Grup de Direccionament i Alliberament Farmacològic, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Pucci F] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Program in Biomedical Sciences, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Otolaryngology-Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America, and Vall d'Hebron Barcelona Hospital Campus
- Subjects
Coll - Càncer ,Multidisciplinary ,Neoplasms::Neoplasms::Neoplasms by Site::Head and Neck Neoplasms::Squamous Cell Carcinoma of Head and Neck [DISEASES] ,células::estructuras celulares::espacio extracelular::vesículas extracelulares [ANATOMÍA] ,Cells::Cellular Structures::Extracellular Space::Extracellular Vesicles [ANATOMY] ,Cap - Càncer ,Cèl·lules canceroses ,neoplasias::neoplasias::neoplasias por localización::neoplasias de cabeza y cuello::carcinoma de células escamosas de cabeza y cuello [ENFERMEDADES] ,células::células madre::células madre neoplásicas [ANATOMÍA] ,Cèl·lules mare ,Cells::Stem Cells::Neoplastic Stem Cells [ANATOMY] - Abstract
Cèl·lules mare del càncer; Càncers de cap i coll; Macròfags Cancer stem cells; Head and neck cancers; Macrophages Células madre del cáncer; Cánceres de cabeza y cuello; Macrófagos Immunotherapy is an approved treatment option for head and neck squamous cell carcinoma (HNSCC). However, the response rate to immune checkpoint blockade is only 13% for recurrent HNSCC, highlighting the urgent need to better understand tumor-immune interplay, with the ultimate goal of improving patient outcomes. HNSCC present high local recurrence rates and therapy resistance that can be attributed to the presence of cancer stem cells (CSC) within tumors. CSC exhibit singular properties that enable them to avoid immune detection and eradication. How CSC communicate with immune cells and which immune cell types are preferentially found within the CSC niche are still open questions. Here, we used genetic approaches to specifically label CSC-derived extracellular vesicles (EVs) and to perform Sortase-mediated in vivo proximity labeling of CSC niche cells. We identified specific immune cell subsets that were selectively targeted by EVCSC and that were found in the CSC niche. Native EVCSC preferentially targeted MHC-II–macrophages and PD1+ T cells in the tumor microenvironment, which were the same immune cell subsets enriched within the CSC niche. These observations indicate that the use of genetic technologies able to track EVs without in vitro isolation are a valuable tool to unveil the biology of native EVCSC. European Molecular Biology Organization (EMBO):Patricia Gonzalez-Callejo short-term fellowship; V Foundation for Cancer Research (VFCR):Natalie M Claudio,Ferdinando Pucci 2019-012.
- Published
- 2023
9. Cancer stem cell-derived extracellular vesicles preferentially target MHC-II-macrophages and PD1+ T cells in the tumor microenvironment.
- Author
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Gonzalez-Callejo P, Guo Z, Ziglari T, Claudio NM, Nguyen KH, Oshimori N, Seras-Franzoso J, and Pucci F
- Subjects
- Humans, Squamous Cell Carcinoma of Head and Neck pathology, T-Lymphocytes pathology, Tumor Microenvironment, Cell Line, Tumor, Neoplasm Recurrence, Local pathology, Neoplastic Stem Cells metabolism, Carcinoma, Squamous Cell pathology, Head and Neck Neoplasms pathology, Extracellular Vesicles pathology
- Abstract
Immunotherapy is an approved treatment option for head and neck squamous cell carcinoma (HNSCC). However, the response rate to immune checkpoint blockade is only 13% for recurrent HNSCC, highlighting the urgent need to better understand tumor-immune interplay, with the ultimate goal of improving patient outcomes. HNSCC present high local recurrence rates and therapy resistance that can be attributed to the presence of cancer stem cells (CSC) within tumors. CSC exhibit singular properties that enable them to avoid immune detection and eradication. How CSC communicate with immune cells and which immune cell types are preferentially found within the CSC niche are still open questions. Here, we used genetic approaches to specifically label CSC-derived extracellular vesicles (EVs) and to perform Sortase-mediated in vivo proximity labeling of CSC niche cells. We identified specific immune cell subsets that were selectively targeted by EVCSC and that were found in the CSC niche. Native EVCSC preferentially targeted MHC-II-macrophages and PD1+ T cells in the tumor microenvironment, which were the same immune cell subsets enriched within the CSC niche. These observations indicate that the use of genetic technologies able to track EVs without in vitro isolation are a valuable tool to unveil the biology of native EVCSC., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Gonzalez-Callejo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2023
- Full Text
- View/download PDF
10. Gene Expression Profiling of Lymph Node Sub-Capsular Sinus Macrophages in Cancer.
- Author
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Pellin D, Claudio N, Guo Z, Ziglari T, and Pucci F
- Subjects
- Animals, Head and Neck Neoplasms immunology, Humans, Lymph Nodes cytology, Mice, Mice, Inbred C57BL, Carcinoma, Squamous Cell immunology, Gene Expression Profiling methods, Lymph Nodes immunology, Macrophages immunology
- Abstract
Lymph nodes are key lymphoid organs collecting lymph fluid and migratory cells from the tissue area they survey. When cancerous cells arise within a tissue, the sentinel lymph node is the first immunological organ to mount an immune response. Sub-capsular sinus macrophages (SSMs) are specialized macrophages residing in the lymph nodes that play important roles as gatekeepers against particulate antigenic material. In the context of cancer, SSMs capture tumor-derived extracellular vesicles (tEVs), a form of particulate antigen released in high amounts by tumor cells. We and others have recently demonstrated that SSMs possess anti-tumor activity because in their absence tumors progress faster. A comprehensive profiling of SSMs represents an important first step to identify the cellular and molecular mechanisms responsible for SSM anti-tumor activity. Unfortunately, the isolation of SSMs for molecular analyses is very challenging. Here, we combined an optimized dissociation protocol, careful marker selection and stringent gating strategies to highly purify SSMs. We provide evidence of decreased T and B cell contamination, which allowed us to reveal the gene expression profile of this elusive macrophage subset. Squamous cell carcinomas induced an increase in the expression of Fc receptors, lysosomal and proteasomal enzymes in SSMs. Imaging of mouse and patient lymph nodes confirmed the presence of the top differentially expressed genes. These results suggest that SSMs respond to tumor formation by upregulating the machinery necessary for presentation of tumor particulate antigens to B cells., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Pellin, Claudio, Guo, Ziglari and Pucci.)
- Published
- 2021
- Full Text
- View/download PDF
11. Contribution of Particle-Induced Lysosomal Membrane Hyperpolarization to Lysosomal Membrane Permeabilization.
- Author
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Ziglari T, Wang Z, and Holian A
- Subjects
- Animals, Cell Death drug effects, Cytosol metabolism, Female, Hydrogen-Ion Concentration, Inflammasomes metabolism, Intracellular Membranes drug effects, Lysosomes drug effects, Macrophages, Alveolar drug effects, Macrophages, Alveolar metabolism, Male, Membrane Potentials drug effects, Mice, Inbred C57BL, Models, Biological, NLR Family, Pyrin Domain-Containing 3 Protein metabolism, Permeability, Potassium metabolism, Reproducibility of Results, Silicon Dioxide toxicity, Mice, Intracellular Membranes physiology, Lysosomes metabolism, Membrane Potentials physiology, Nanoparticles toxicity
- Abstract
Lysosomal membrane permeabilization (LMP) has been proposed to precede nanoparticle-induced macrophage injury and NLRP3 inflammasome activation; however, the underlying mechanism(s) of LMP is unknown. We propose that nanoparticle-induced lysosomal hyperpolarization triggers LMP. In this study, a rapid non-invasive method was used to measure changes in lysosomal membrane potential of murine alveolar macrophages (AM) in response to a series of nanoparticles (ZnO, TiO
2 , and CeO2 ). Crystalline SiO2 (micron-sized) was used as a positive control. Changes in cytosolic potassium were measured using Asante potassium green 2. The results demonstrated that ZnO or SiO2 hyperpolarized the lysosomal membrane and decreased cytosolic potassium, suggesting increased lysosome permeability to potassium. Time-course experiments revealed that lysosomal hyperpolarization was an early event leading to LMP, NLRP3 activation, and cell death. In contrast, TiO2 - or valinomycin-treated AM did not cause LMP unless high doses led to lysosomal hyperpolarization. Neither lysosomal hyperpolarization nor LMP was observed in CeO2 -treated AM. These results suggested that a threshold of lysosomal membrane potential must be exceeded to cause LMP. Furthermore, inhibition of lysosomal hyperpolarization with Bafilomycin A1 blocked LMP and NLRP3 activation, suggesting a causal relation between lysosomal hyperpolarization and LMP.- Published
- 2021
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12. Determination of the relative contribution of the non-dissolved fraction of ZnO NP on membrane permeability and cytotoxicity.
- Author
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Ziglari T, Anderson DS, and Holian A
- Subjects
- Animals, Erythrocytes drug effects, Female, Hemolysis drug effects, Humans, Lysosomes metabolism, Macrophages drug effects, Male, Mice, Inbred C57BL, Nanoparticles chemistry, Solubility, THP-1 Cells, Zinc Oxide chemistry, Cell Membrane Permeability drug effects, Cell Survival drug effects, Nanoparticles toxicity, Zinc Oxide toxicity
- Abstract
Background: While the role of lysosomal membrane permeabilization (LMP) in NP-induced inflammatory responses has been recognized, the underlying mechanism of LMP is still unclear. The assumption has been that zinc oxide (ZnO)-induced LMP is due to Zn
2+ ; however, little is known about the role of ZnO nanoparticles (NP) in toxicity. Methods: We examined the contribution of intact ZnO NP on membrane permeability using red blood cells (RBC) and undifferentiated THP-1 cells as models of particle-membrane interactions to simulate ZnO NP-lysosomal membrane interaction. The integrity of plasma membranes was evaluated by transmission electron microscopy (TEM) and confocal microscopy. ZnO NP dissolution was determined using ZnAF-2F, Zn2+ specific probe. The stability of ZnO NP inside the phagolysosomes of phagocytic cells, differentiated THP-1, alveolar macrophages, and bone marrow-derived macrophages, was determined. Results: ZnO NP caused significant hemolysis and cytotoxicity under conditions of negligible dissolution. Fully ionized Zn2 SO4 caused slight hemolysis, while partially ionized ZnO induced significant hemolysis. Confocal microscopy and TEM images did not reveal membrane disruption in RBC and THP-1 cells, respectively. ZnO NP remained intact inside the phagolysosomes after a 4 h incubation with phagocytic cells. Conclusions: These studies demonstrate the ability of intact ZnO NP to induce membrane permeability and cytotoxicity without the contribution of dissolved Zn2+ , suggesting that ZnO NP toxicity does not necessarily depend upon Zn2+ . The stability of ZnO NP inside the phagolysosomes suggests that LMP is the result of the toxic effect of intact ZnO NP on phagolysosomal membranes.- Published
- 2020
- Full Text
- View/download PDF
13. Comparison of glutathione S-transferase activity and concentration in aflatoxin-producing and their non-toxigenic counterpart isolates.
- Author
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Ziglari T, Allameh A, Razzaghi-Abyaneh M, Khosravi AR, and Yadegari MH
- Subjects
- Aspergillus enzymology, Aspergillus isolation & purification, Aspergillus flavus enzymology, Aspergillus flavus isolation & purification, Chromatography, Affinity, Enzyme-Linked Immunosorbent Assay, Mycelium enzymology, Mycelium metabolism, Species Specificity, Aflatoxins biosynthesis, Aspergillus metabolism, Aspergillus flavus metabolism, Glutathione Transferase metabolism
- Abstract
In this study, two techniques were used to compare the specific activity and total concentration of mycelial glutathione S-transferase (GST) in fungal strains isolated from natural sources. The fungi identified as Aspergillus parasiticus and Aspergillus flavus have been divided into two groups based on their ability to produce aflatoxins. Altogether 26 fungi were isolated, among which 12 were capable of producing varying levels of aflatoxin and 14 were proved to be non-toxigenic. GST specific activity in mycelial preparation was measured spectrophotometrically using 2,1-chloro-2,4-dinitrobenzene as the substrate. The results showed that the mean GST activity in toxigenic isolates was 25.06 +/- 9.8 micromol/mg protein/min which was 2.8-fold greater than that measured in non-toxigenic isolates (8.84 +/- 5.5 micromol/mg protein/min). Moreover, the GST concentration was compared in toxigenic and non-toxigenic isolates using an Enzyme Linked Immunosorbent Assay based on antigen (fungal preparation) and antibody (antibody produced against fungal GST in rabbit). The results of ELISA showed that the mean GST level in toxigenic and non-toxigenic fungi was 1.17 +/- 0.55 and 0.40 +/- 0.24, respectively. These results further confirm that the aflatoxin production in the fungal strains is correlated with GST expression and using ELISA, it is possible to discriminate aflatoxin-producing fungi from their non-toxigenic counterparts.
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- 2008
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14. Unusual presentation of tinea cruris due to Trichophyton mentagrophytes var. mentagrophytes.
- Author
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Khosravi AR, Mansouri P, Naraghi Z, Shokri H, and Ziglari T
- Subjects
- Adult, Dermatomycoses drug therapy, Dermatomycoses microbiology, Humans, Itraconazole administration & dosage, Male, Manganese Compounds administration & dosage, Oxides administration & dosage, Pulse Therapy, Drug, Tinea drug therapy, Tinea microbiology, Treatment Outcome, Dermatomycoses diagnosis, Dermatomycoses pathology, Skin pathology, Tinea diagnosis, Tinea pathology, Trichophyton isolation & purification
- Abstract
Tinea cruris is a subacute or chronic dermatophytosis. This report describes a 30-year-old man presenting with a history of bullous erythematous along with itching of his crural regions. On the basis of clinical findings, the lesions were diagnosed as contact dermatitis and pustular psoriasis, so he was treated by local and systemic corticosteroids but pustular lesions were progressed on the affected sites. By using mycological and histological examinations, dermatophytosis caused by Trichophyton mentagrophytes var. mentagrophytes was confirmed. The patient was successfully treated with itraconazole and local antifungal treatments.
- Published
- 2008
- Full Text
- View/download PDF
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