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Abstract Explore whether the axillary outcomes differ among HER2 positive subgroups receiving standard dual-targeted therapy, aiming to identify subgroups exhibiting enhanced sensitivity to NAT among HER2-positive/node-positive breast cancer patients. HER2 positive female patients with biopsy-proven node-positive disease from April 2020 to May 2023 were included. All patients underwent standard Neoadjuvant HER2-targeted dual therapy and axillary lymph node dissection (ALND) at Breast Surgery Center of Sichuan Cancer Hospital. Univariate and multivariate analyses were used to identify factors associate with axillary pathological complete response (ApCR). Statistical analysis and graphing were performed using SPSS 24.0 and GraphPad Prism 9.0 software. This study enrolled 215 HER2 positive patients with a total ApCR rate of 76.7%, which included 49 HER2 2+/FISH + and 166 HER2 3 + cases with approximate ApCR rates of 63.3% and 80.7% (P = 0.011). Univariate and multivariate analysis indicated that HER2 3 + disease (OR = 2.43, 95% CI 1.21–4.88, P = 0.012), Ki-67 ≥ 20% disease (OR = 3.00, 95% CI 1.26–7.13, P = 0.013) and NAC regimen of TCb (OR = 2.71, 95% CI 1.39–5.38, P = 0.004) were more likely to achieve ApCR. Further subgroup analysis revealed that HER2 3 + patients receiving TCb regimen showed the highest ApCR rate of 88% compared to other subgroups. HER2 3 + breast cancer had a higher ApCR rate than HER2 2+/FISH + breast cancer during Neoadjuvant HER2-targeted dual therapy. HER2 positive patients could benefit from NAC regimen of TCb in axillary response.

Abstract Background Human health is a complex, dynamic concept encompassing a spectrum of states influenced by genetic, environmental, physiological, and psychological factors. Traditional Chinese Medicine categorizes health into nine body constitutional types, each reflecting unique balances or imbalances in vital energies, influencing physical, mental, and emotional states. Advances in machine learning models offer promising avenues for diagnosing conditions like Alzheimer's, dementia, and respiratory diseases by analyzing speech patterns, enabling complementary non-invasive disease diagnosis. The study aims to use speech audio to identify subhealth populations characterized by unbalanced constitution types. Methods Participants, aged 18–45, were selected from the Acoustic Study of Health. Audio recordings were collected using ATR2500X-USB microphones and Praat software. Exclusion criteria included recent illness, dental issues, and specific medical histories. The audio data were preprocessed to Mel-frequency cepstral coefficients (MFCCs) for model training. Three deep learning models—1-Dimensional Convolution Network (Conv1D), 2-Dimensional Convolution Network (Conv2D), and Long Short-Term Memory (LSTM)—were implemented using Python to classify health status. Saliency maps were generated to provide model explainability. Results The study used 1,378 recordings from balanced (healthy) and 1,413 from unbalanced (subhealth) types. The Conv1D model achieved a training accuracy of 91.91% and validation accuracy of 84.19%. The Conv2D model had 96.19% training accuracy and 84.93% validation accuracy. The LSTM model showed 92.79% training accuracy and 87.13% validation accuracy, with early signs of overfitting. AUC scores were 0.92 and 0.94 (Conv1D), 0.99 (Conv2D), and 0.97 (LSTM). All models demonstrated robust performance, with Conv2D excelling in discrimination accuracy. Conclusions The deep learning classification of human speech audio for health status using body constitution types showed promising results with Conv1D, Conv2D, and LSTM models. Analysis of ROC curves, training accuracy, and validation accuracy showed all models robustly distinguished between balanced and unbalanced constitution types. Conv2D excelled with good accuracy, while Conv1D and LSTM also performed well, affirming their reliability. The study integrates constitution theory and deep learning technologies to classify subhealth populations using noninvasive approach, thereby promoting personalized medicine and early intervention strategies.

Abstract Cell-cell communication is a key aspect of dissecting the complex cellular microenvironment. Existing single-cell and spatial transcriptomics-based methods primarily focus on identifying cell-type pairs for a specific interaction, while less attention has been paid to the prioritisation of interaction features or the identification of interaction spots in the spatial context. Here, we introduce SpatialDM, a statistical model and toolbox leveraging a bivariant Moran’s statistic to detect spatially co-expressed ligand and receptor pairs, their local interacting spots (single-spot resolution), and communication patterns. By deriving an analytical null distribution, this method is scalable to millions of spots and shows accurate and robust performance in various simulations. On multiple datasets including melanoma, Ventricular-Subventricular Zone, and intestine, SpatialDM reveals promising communication patterns and identifies differential interactions between conditions, hence enabling the discovery of context-specific cell cooperation and signalling.

The incorporation of carbon nanotubes (CNTs) can enhance the mechanical properties of concrete. The stress–strain curves of CNT-reinforced concrete under uniaxial compression are investigated through an experimental program with different CNT and steel fiber proportions considered. The test results demonstrate that CNTs can increase both peak stress and peak strain, and steel fibers can further enhance the effect of CNTs. Additionally, steel fibers can effectively enhance both the strength and ductility. Theoretical models for the peak strain, initial elastic modulus, toughness index and relative absorbed energy are established. A theoretical model for the uniaxial compressive constitutive relationship of CNT-reinforced concrete considering CNT and steel fiber content is developed. Finite element (FE) modelling is developed to simulate the axial compression behavior of CNT-reinforced concrete.

Filtering from airborne LiDAR datasets in urban area is one important process during the building of digital and smart cities. However, the existing filters encounter poor filtering performance and heavy computational burden when processing large-scale and complicated urban environments. To tackle this issue, a self-adaptive filtering method based on object primitive global energy minimization is proposed in this paper. In this paper, mode points were first acquired for generating the mode graph. The mode points were the cluster centers of the LiDAR data obtained in a mean shift algorithm. The graph constructed with mode points was named “mode graph” in this paper. By defining the energy function based on the mode graph, the filtering process is transformed to iterative global energy minimization. In each iteration, the graph cuts technique was adopted to achieve global energy minimization. Meanwhile, the probability of each point belonging to the ground was updated, which would lead to a new refined ground surface using the points whose probabilities were greater than 0.5. This process was iterated until two successive fitted ground surfaces were determined to be close enough. Four urban samples with different urban environments were adopted for verifying the effectiveness of the filter developed in this paper. Experimental results indicate that the developed filter obtained the best filtering performance. Both the total error and the Kappa coefficient are superior to those of the other three classical filtering methods.

PurposeTo evaluate whether changes in ultrasound features during neoadjuvant chemotherapy (NAC) could predict axillary node response in clinically node-positive breast cancer patients.MethodsPatients with biopsy-proven node-positive disease receiving NAC between February 2009 and March 2021 were included. Ultrasound (US) images were obtained using a 5-12-MHz linear array transducer before NAC, after two cycles, and at the completion of NAC. Long and short diameter, cortical thickness, vascularity, and hilum status of the metastatic node were retrospectively reviewed according to breast imaging-reporting and data system (BI-RADS). The included population was randomly divided into a training set and a validation set at a 2:1 ratio using a simple random sampling method. Factors associated with node response were identified through univariate and multivariate analyses. A nomogram combining clinical and changes in ultrasonographic (US) features was developed and validated. The receiver operating characteristic (ROC) and calibration plots were applied to evaluate nomogram performance and discrimination.ResultsA total of 296 breast cancer patients were included, 108 (36.5%) of whom achieved axillary pathologic complete response (pCR) and 188 (63.5%) had residual nodal disease. Multivariate regression indicated that independent predictors of node pCR contain ultrasound features in addition to clinical features, clinical features including neoadjuvant HER2-targeted therapy and clinical response, ultrasound features after NAC including cortical thickness, hilum status, and reduction in short diameter ≥50%. The nomogram combining clinical features and US features showed better diagnostic performance compared to clinical-only model in the training cohort (AUC: 0.799 vs. 0.699, P=0.001) and the validation cohort (AUC: 0.764 vs. 0.638, P=0.027).ConclusionsUltrasound changes during NAC could improve the accuracy to predict node response after NAC in clinically node-positive breast cancer patients.

Abstract The stray current of direct current (DC) railway systems causes magnetic disturbance in geomagnetic measurements, which may complicate the identification of useful information. The magnetic disturbance exhibits broadband characteristics in the frequency domain. In this paper, we propose a noise reduction method based on the adaptive Kalman filter to extract useful signals from the geomagnetic data with a high level of noise. The covariance matrixes of both the process noise (Q) and measurement noise (R) can be adaptively estimated to improve the performance of the adaptive Kalman filter. The proposed method is adopted to process the geomagnetic data collected at the Beijing Geomagnetic Observatory (BJI), which is affected by the DC railway system. The magnetic disturbance is largely reduced, and the signal-to-noise ratios of the horizontal and vertical components of the geomagnetic field are improved by more than 14 dB and 27 dB, respectively. The K-indices are calculated to evaluate the performance of the adaptive Kalman filter method. To assess the influence of the adaptive Kalman filter on natural signals, the geomagnetic data that contain rapid variations are processed. The denoising results show that the adaptive Kalman filter can effectively reduce the magnetic disturbance caused by DC railway system without large impact on the natural geomagnetic rapid variations.

Twin-wire and arc additive manufacturing (T-WAAM) has potential advantages in improving deposition efficiency and manufacturing functionally graded materials (FGMs), thus attracting much attention. However, there are few studies on the droplet transfer mode of T-WAAM. This paper analyzes the droplet transfer mode and arc swing in the “TIG + AC” twin-wire cross-arc additive manufacturing by in-situ observation with high-speed photography, revealing what factors influence the T-WAAM on deposition shaping the quality and what are the key mechanisms for process stability. Experiments show that with the main arc current provided by TIG 100 A and the twin-wire AC arc current 10 A, three different droplet transfer modes, namely the “free transfer + free transfer, bridge transfer + free transfer, bridge transfer + bridge transfer,” can be observed with the twin wires under different feeding speeds. The corresponding deposition and arc swing are quite different in quality. Through comparative analysis, it is found that the frequent extinguishment and ignition of the arc between electrode wires is the main factor for the instability in the additive manufacturing process. The “bridge transfer + free transfer” mode can obtain a large arc swing angle and a stable deposition, in which the cross arc has a significant stirring effect on the molten pool, and the deposition shape is well-made.

The rutting depth is an important index to evaluate the damage degree of the pavement. Therefore, establishing an accurate rutting depth prediction model can guide pavement design and provide the necessary basis for pavement maintenance. However, the sample size of pavement rutting depth data is small, and the sampling is not standardized, which makes it hard to establish a prediction model with high accuracy. Based on the data of RIOHTrack’s asphalt pavement structure, this study builds a reliable data-augmented model. In this paper, different asphalt rutting data augmented models based on Gaussian radial basis neural networks are constructed with the temperature and loading of asphalt pavements as the main features. Experimental results show that the method outperforms classical machine learning methods in data augmentation, with an average root mean square error of 3.95 and an average R-square of 0.957. Finally, the augmented data of rutting depth is constructed for training, and multiple neural network models are used for prediction. Compared with unaugmented data, the prediction accuracy is increased by 50%.

Peripheral nerve injury (PNI) is a common clinical problem, which can cause severe disability and dramatically affect a patient’s quality of life. Neural regeneration after PNI is a complex biological process that involves a variety of signaling pathways and genes. Emerging studies demonstrated that long non-coding RNAs (lncRNAs) were abnormally expressed after PNI and played pivotal roles in peripheral nerve regeneration. Based on the rat sciatic nerve injury model, we found that the expression levels of several lncRNAs were increased significantly in the sciatic nerve after injury. Software prediction prompted us to focus on one up-regulated lncRNA, MSTRG.24008.1. Dual-luciferase reporter assay, RNA pull-down assay and RNA interference approach verified that MSTRG.24008.1 regulated neuroregeneration via the miR-331-3p/nucleotide-binding oligomerization domain-like pyrin domain containing 3 (NLRP3)/myelin and lymphocyte protein (MAL) axis in vitro. Subsequently, we performed gastrocnemius muscle gravity and sciatic functional index experiments to evaluate the recovery of injured sciatic nerves after MSTRG.24008.1 siRNA interference in vivo. In conclusion, knockdown of MSTRG.24008.1 promotes the regeneration of the sciatic nerve via the miR-331-3p/NLRP3/MAL axis, which may provide a new strategy to evaluate and repair injured peripheral nerves clinically.

Having upended the traditional software development, which historically was centred exclusively on proprietary, copyright-protected code, open-source has now entered the physical artefact world. In doing so, it has started to change not only how physical products are designed and developed, but also the commercialisation process. In recent years, authors have witnessed entrepreneurs intentionally choosing not to patent their product design and technologies but instead licencing the designs and technologies under open-source licences. The entrepreneurs share their product designs online with their community – people who congregated due to the shared interests in products’ technology or project’s social objectives. Founding a startup firm without excluding others from using their own invention is not a common practice. Therefore, there is reason to ask if this choice a strategic decision or irrational action due to short-sightedness or extreme altruism? Conducting interviews with 65 founders, we grounded a framework explaining that the driver of going open is a result of both intrinsic and extrinsic factors. In addition, we observed the change of identities over time among the entrepreneurs. We hope to use this paper as a pilot study of this emerging socio-technological phenomenon, which is understudied relative to the proprietary product commercialisation process.

Individual tree modeling for terrestrial LiDAR point clouds always involves heavy computation burden and low accuracy toward a complex tree structure. To solve these problems, this paper proposed a self-adaptive optimization individual tree modeling method. In this paper, we first proposed a joint neighboring growing method to segment wood points into object primitives. Subsequently, local object primitives were optimized to alleviate the computation burden. To build the topology relation among branches, branches were separated based on spatial connectivity analysis. And then the nodes corresponding to each object primitive were adopted to construct the graph structure of the tree. Furthermore, each object primitive was fitted as a cylinder. To revise the local abnormal cylinder, a self-adaptive optimization method based on the constructed graph structure was proposed. Finally, the constructed tree model was further optimized globally based on prior knowledge. Twenty-nine field datasets obtained from three forest sites were adopted to evaluate the performance of the proposed method. The experimental results show that the proposed method can achieve satisfying individual tree modeling accuracy. The mean volume deviation of the proposed method is 1.427 m3. In the comparison with two other famous tree modeling methods, the proposed method can achieve the best individual tree modeling result no matter which accuracy indicator is selected.

Building extraction from airborne Light Detection and Ranging (LiDAR) point clouds is a significant step in the process of digital urban construction. Although the existing building extraction methods perform well in simple urban environments, when encountering complicated city environments with irregular building shapes or varying building sizes, these methods cannot achieve satisfactory building extraction results. To address these challenges, a building extraction method from airborne LiDAR data based on multi-constraints graph segmentation was proposed in this paper. The proposed method mainly converted point-based building extraction into object-based building extraction through multi-constraints graph segmentation. The initial extracted building points were derived according to the spatial geometric features of different object primitives. Finally, a multi-scale progressive growth optimization method was proposed to recover some omitted building points and improve the completeness of building extraction. The proposed method was tested and validated using three datasets provided by the International Society for Photogrammetry and Remote Sensing (ISPRS). Experimental results show that the proposed method can achieve the best building extraction results. It was also found that no matter the average quality or the average F1 score, the proposed method outperformed ten other investigated building extraction methods.

Recovery of iron from iron-rich diasporic bauxite ore via reductive roasting followed by magnetic separation has been explored recently. However, the efficiency of alumina extraction in the non-magnetic materials is absent. In this paper, a further study on the digestion of alumina by the Bayer process from non-magnetic material obtained after magnetic separation of reduced iron-rich diasporic bauxite with sodium salts was investigated. The results indicate that the addition of sodium salts can destroy the original occurrences of iron-, aluminum- and silicon-containing minerals of bauxite ore during reductive roasting. Meanwhile, the reactions of sodium salts with complex aluminum- and silicon-bearing phases generate diaoyudaoite and sodium aluminosilicate. The separation of iron via reductive roasting of bauxite ore with sodium salts followed by magnetic separation improves alumina digestion in the Bayer process. When the alumina-bearing material in bauxite ore is converted into non-magnetic material, the digestion temperature decreases significantly from 280 °C to 240 °C with a nearly 99% relative digestion ratio of alumina.