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Your search keyword '"Zhuo Yinghong"' showing total 2 results
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2 results on '"Zhuo Yinghong"'

1. Biocompatiable silk fibroin/carboxymethyl chitosan/strontium substituted hydroxyapatite/cellulose nanocrystal composite scaffolds for bone tissue engineering

Author

Zhang Xiaoyun, Yue-Ping Chen, Dong Panfeng, Jie Han, Jian Mo, Feng Yang, and Zhuo Yinghong

Subjects
Scaffold, Artificial bone, Cell Survival, Fibroin, Biocompatible Materials, 02 engineering and technology, Bone healing, Biochemistry, Bone and Bones, Nanocomposites, 03 medical and health sciences, chemistry.chemical_compound, stomatognathic system, Structural Biology, Osteogenesis, Materials Testing, Humans, Cellulose, Bone regeneration, Molecular Biology, 030304 developmental biology, 0303 health sciences, Chitosan, Tissue Engineering, Tissue Scaffolds, Chemistry, General Medicine, 021001 nanoscience & nanotechnology, Chemical engineering, Strontium, Nanoparticles, Hydroxyapatites, Biocomposite, 0210 nano-technology, Fibroins, Protein adsorption
Abstract

Bone defects arise from trauma, skeletal diseases or tumor resections have become a critical clinical challenge. Biocomposite materials as artificial bone repair materials provide a promising approach for bone regeneration. In this study, we used silk fibroin (SF), carboxymethyl chitosan (CMCS), cellulose nanocrystals (CNCs) and strontium substituted hydroxyapatite (Sr-HAp) to prepare the biocomposite scaffolds of SF/CMCS, SF/CMCS/CNCs, SF/CMCS/CNCs/Sr-HAp. The characterization results showed that all the SF-based scaffolds have a porous sponge-like structure with porosities over 80%. In addition, there was a significant increase in compressive strength of SF/CMCS/Sr-HAp/CNCs scaffold when compared to that of SF/CMCS scaffolds, while maintaining high porosity with lower swelling ratio. All the SF-based scaffolds were non-toxic and had a good hemocompatibility. Comparing to the SF/CMCS scaffold, the scaffolds with addition of Sr-HAp and/or CNCs showed enhanced protein adsorption and ALP activity. In addition, higher expression of osteogenic gene markers such as RUNX2, ALP, OCN, OPN, BSP and COL-1 further substantiated the applicability of SF/CMCS/Sr-HAp/CNCs scaffolds for bone related applications. Hence, this study suggests that SF/CMCS/Sr-HAp/CNCs scaffolds have a potential in non-loading bone repair application.

Published
2019

2. Good cell compatibility and permeability of silk fibroin/chitosan composite scaffolds.

Author

Zhang Xiaoyun, Chen Yueping, Song Shilei, Zhang Chi, Zhuo Yinghong, Yang Nan, Zhan Huasong, and Xu Canhong

Subjects
SILK fibroin, CELL permeability, NUCLEAR magnetic resonance, PARTICLE size distribution, CO-cultures
Abstract

BACKGROUND: Silk fibroin and chitosan are commonly used as scaffolds in tissue engineering, but there are some shortcomings in their separate application. When they are mixed, they can be modified each other. They give full play to each other's advantages and become ideal composite scaffolds. OBJECTIVE: To prepare Silk fibroin/chitosan composite scaffold and determineits properties. METHODS: The silk fibroin/chitosan composite scaffolds were prepared by freeze-drying method. The morphology and structure of the composite scaffolds were examined by scanning electron microscopy. The properties of the composite scaffolds were tested by thermogravimetric analysis, mechanical properties test, and cytotoxicity test. The quaternion chitosan was prepared. The nuclear magnetic resonance spectrum was detected by nuclear magnetic resonance instrument. The potential and particle size distribution were detected by Zeta potentiometer. The protection of DNA was detected by gel eledrophoresis. The binding with DNA was observed by transmission electron microscope. RESULTS AND CONCLUSION: (1) The results of scanning electron microscopy showed that the silk fibroin/chitosan composite scaffolds had a good three-dimensional pore structure, with a pore size of 50-100 urn. (2) The results of thermogravimetric analysis showed that when the temperature was less than 200 °C, the mass loss rate of silk fibroin/chitosan composite scaffold was lower. When the temperature increased to 200-500 °C, the mass loss rate of the scaffold began to accelerate, and the loss amount increased. At 800 °C, the residual mass of the composite scaffold was 38%. (3) The maximum strain of silk fibroin/chitosan composite scaffold reached 94.94%, and the maximum stress was 7.01 MPa. (4) The results of CCK-8 experiment showed that silk fibroin/chitosan composite scaffolds had no cytotoxicity to rabbit bone marrow mesenchymal stem cells and had good cell compatibility. (5) The results of nuclear magnetic resonance spectra showed that the quaternion degree of quaternary ammonium chitosan was about 20%. (6) The particle size distribution of quatemized chitosan was (588.56±52.39) nm, and the surface of quatemized chitosan was positively charged with a potential of (16.3±3.92) mV, which was beneficial to the combination with DNA. (7) The results of gel electrophoresis experiments showed that the higher the proportion of quaternion chitosan, the better the encapsulation of DNA. When the ratio of chitosan/DNA was 1 : 3, the encapsulation effect was achieved. (8) The results of transmission electron microscopy showed that most of the particles of quatemized chitosan/DNA were solid and round; the particle size difference was small; and the average particle size was about 200 nm. (9) The results showed that silk fibroin/chitosan composite scaffolds had a good biocompatibility and cell permeability, which was conducive to the growth of cells between scaffolds. [ABSTRACT FROM AUTHOR]

Published
2020
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