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Abstract Background Blastocystis is ubiquitous presence in animals and humans worldwide and has a high level genetic diversity. The aim of this study was to conduct a summary of Blastocystis prevalence, subtypes (STs) in humans and animals in China and depict their distribution. Methods We searched for the articles related to epidemiology of Blastocystis in humans and animals throughout China which published from January 1, 1990, to July 31, 2019 in the following databases: PubMed, China National Knowledge Infrastructure (CNKI) and Wanfang database. The keywords were Blastocystis and one of the following ones: STs, subtypes, distribution, epidemiology, prevalence, infection, molecular, geographic, intestinal parasites, genetic diversity and characterization. Results In recent years, various molecular epidemiological studies have been carried out in some provinces/regions of China to identify subtypes of Blastocystis. Infants and young children, school students, hospitalized diarrhea patients, HIV/AIDS patients, tuberculosis patients, and cancer patients as respondents had been included. ST1–ST7 and ST12 were the main subtypes in Chinese population. Moreover, surveys of Blastocystis infection in animal were also conducted in some provinces of China. A variety of animals were investigated including pigs, cattle, sheep, yak, giant panda, and crested ibis (Nipponia nippon) with the main subtypes of ST1–ST8, ST10, ST12–ST14. Conclusions In recent years, some provinces/regions in China have conducted various molecular epidemiological studies to identify the Blastocystis subtypes. It is important to focus on new subtypes and mixed subtypes of infection, while increasing data on ribosomal alleles. We encourage the scientific community to start research on humans and surrounding animals (including domestic and wild animals) to better understand the possibility of Blastocystis transmission between humans and animals. We call for action among researchers studying intestinal parasitic diseases (Blastocystis), start drawing the subtype of Blastocystis and increase the subtype related to its clinical symptoms.

Objective: To develop a bionic animal skin and evaluate its detective effect of Schistosoma japonicum cercariae so as to provide a new technique for the monitoring of the schistosome susceptibility water body., Methods: A bionic animal skin was made with pigskin and the experiments were carried out for evaluating its detective effects of S. japonicum cercariae in laboratory and simulate fields, and at the same time, the sentinel mouse method was used as the control., Results: In the laboratory, the cercariae were found in 10-, 30-, 60-cercaria subgroups in the bionic animal skin group after 1 and 2 hours, but the cercariae were found only in the 60-cercaria subgroup in the sentinel mouse group. In the simulate fields, in the water body with 5 schistosome-infected Oncomelania hupensis snails, the cercariae were found in 2 bionic animal skin devices (2/4) and 3 bionic animal skin devices (3/4) after 2 hours and 4 hours respectively, and in the water body with 10 schistosome-infected O. hupensis snails, the cercariae were found in 4 bionic animal skin devices (4/4) and 3 bionic animal skin devices (3/4) after 2 hours and 4 hours respectively in the bionic animal skin group. In the the sentinel mouse group, in the above-mentioned water bodies (with 5 or 10 schistosome-infected O. hupensis snails), the cercariae were found in 2 sentinel mice (2/4) and 1 sentinel mice (1/4) after 2 hours and 4 hours respectively; and the cercariae were found in 2 sentinel mice (2/4) and 3 sentinel mice (3/4) after 2 hours and 4 hours respectively., Conclusions: The bionic animal skin device can be used to detect the S. japonicum cercariae, and its sensibility and efficiency are superior to the traditional sentinel mouse method.

Objective: To understand the present situation of the chronic schistosomiasis patients' knowledge, attitude and practice on schistosomiasis control in Nanchang City., Methods: The knowledge, attitude and values on schistosomiasis control of 523 chronic schistosomiasis patients in Nanchang County, Jinxian County and Xinjian District in the Poyang Lake District were investigated with questionnaires. And the accuracy rates of the knowledge, attitude and practice among the patient groups of different counties, genders, age groups, occupations and educational levels were analyzed., Results: The accuracy rates of the knowledge, attitude and practice of patients on schistosomiasis control were 95.76%, 82.80%, and 81.73% in Nanchang County; 91.37%, 93.32%, and 76.48% in Jinxian County; 88.25%, 67.56%, and 49.40% in Xinjian District. In the accuracy rates of knowledge, attitude and practice, the differences among the three counties (districts) were statistically significant ( χ 2 = 57.511-301.378, all P < 0.05) ., Conclusions: The accuracy rates of chronic schistosomiasis patients' attitude and practice on schistosomiasis control in Nanchang City remain low. Therefore, the intensity of attitude and practice intervention should be strengthened in the Poyang Lake District in order to enhance the self-protection awareness of the patients.

Objective: To investigate the endemic situation of human Toxoplasma gondii infection in Nanchang City in 2014 and analyze the influencing factors, so as to provide the evidence for control measures., Methods: The high-risk population of T. gondii infection in Nanchang City was collected as an experiment group, and a control group was settled with the ratio of 1∶1. The serum tests and epidemiology surveys were conducted simultaneously, and the influencing factors were analyzed., Results: The total positive rate of human T. gondii was 5.17% (124/2 400) among the whole population in Nanchang City in 2014. The positive rate was 7.50% (90/1 200) in the experiment group and 2.18% (34/1 200) in the control group, and the difference was significant ( χ 2 =26.668, P ＜0.05). The results of univariate analysis indicated that different dietary habits and health habits were closely related to the infection of T. gondii ( χ 2 =16.522, 64.954, both P ＜0.05). The result of Logistic analysis showed that the risk factors of T. gondii infection were occupation, educational levels, raising cats, contacting animals or raw meat, and eating raw or uncooked beef., Conclusions: The high risk population of T. gondii infection is the key population of the toxoplasmosis control. Unhealthy dietary and living habits are key influencing factors of toxoplasmosis, and the related health education and surveillance work should be strengthened.