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Objective With the increasing automation levels in urban rail transit, drivers responsible for operation in GOA2 (semi-automated train operation) mode become the multitasking personnel responsible for monitoring and inspecting compartments in GOA4 (unattended train operation) mode. The emergency disposal operation procedure has changed as a result. To achieve a more refined analysis and evaluation of urban rail transit train operations in the GOA4 mode, it is necessary to conduct an in-depth study on the quantitative assessment method for the emergency disposal operation complexity in terms of key driving post personnel. Method Based on standardized operating procedures, the WDA (work domain analysis) method is used to establish an information structure diagram for key driving post personnel. The emergency disposal operation complexity in terms of key driving post personnel under both GOA2 and GOA4 modes is analyzed from four aspects of information quantity, operation logic, operational scale, and overall complexity. Using door malfunction emergency disposal operations as example, the emergency disposal operation complexity for key driving post personnel in both modes is calculated, and two-tailed Pearson test is carried out for site standardized operational score rate correlation verification. Result & Conclusion The correlation test results show that the correlation coefficient of vehicle door fault emergency disposal operations is -0.827, significance level is 0.042, the complexity of the operation and the score rate is negatively correlated, validating the effectiveness of the SC (step complexity) model.

【Objective】 The fruit radish is a cash crop commonly cultivated in greenhouses in central and northern China. This study aims to determine the most effective drip irrigation schedule to enhance its yield while simultaneously increasing water use efficiency. 【Method】 The cultivar “Ice Cream” was used as the model plant. The irrigation was based on soil matric potential measured at the depth of 20 cm in that whenever it dropped to -10 (T1), -20 (T2), -30 (T3), -40 (T4) or -50 kPa (T5), we irrigated the crops by 10 mm of water. In each treatment, we measured the growth, yield and quality, as well as irrigation water use efficiency (IWUE) of the crop. 【Result】 The total irrigation amount for T1-T5 decreased from 120 to 70 mm. Reducing the soil matric potential threshold for irrigation resulted in reductions in area and fresh weight of leaves, root diameter and length, and weight of the radish, albeit the reduction in root length was not significant. Compared with T1, T2—T5 increased the yield by 2.2% to 44.0%, T2 increased IWUE by 17.3%, and T3—T5 reduced IWUE by 4.1% to 6.6%. With the decrease in the soil matric potential threshold, the soluble solid content and electrical conductivity of the radish both increased first followed by a decline, the soluble solid content peaked in T2 and Vc content increased monotonically. 【Conclusion】 Setting soil matric potential at depth of 20 cm at -20 kPa as the threshold and the irrigating 10 mm of water each time is optimal for improving the yield and IWUE of greenhouse fruit radish.

As an important measure to improve the supply of essential health services, the implementation of hierarchical medical system has encountered obstacles in different regions. Due to different referral standards in hospitals, temporal and geographical factors during treatment, it is difficult to promote the development of regional medical consortiums. Shanghai Pudong New Area People's Hospital, together with seven community health centers, established a Chuansha Medical Consortium, specifically for treating chronic kidney disease (CKD), using an accurate hierarchical management program for CKD supported by information technology, a hierarchical diagnosis and treatment system designed based on CKD management programs recommended by relevant guidelines, a CKD knowledge base in which medical information and referral criteria are incorporated, and a joint management model integrating hospital and community care. Empirical evaluation indicated that the hierarchical diagnosis and treatment model based on a CKD knowledge base could greatly contributed to accurately identifying vulnerable and high-risk population of CKD, timely screening and assessing patients with early stage CKD, and improving the efficiency of health management and diagnosis and treatment.

Objective To investigate the clinical characteristics, treatment and prognosis of the eighth edition of AJCC stage Ⅲ gallbladder cancer (GBC). Methods We collected the clinical data and follow-up results of 3485 patients with AJCC 8th stage Ⅲ gallbladder cancer. Kaplan Meier survival curves of ⅢA and ⅢB, T3N0M0 (ⅢA), T1-2N1M0 (ⅢB) and T3N1M0 (ⅢB) were drawn and compared. Single factor analysis and Cox multiple factor regression analysis were used to analyze the relation between clinical characteristics, treatment plan, stage Ⅲ subtype and prognosis. Results One-year survival rate of stage ⅢB gallbladder cancer patients was 49.70%, higher than those of stage ⅢA(36.41%); the 1-year survival rate of stage T1-2N1M0 (ⅢB) gallbladder cancer patients was 65.52%, higher than those of stage T3N0M0 (ⅢA) (36.41%) and stage T3N1M0 (ⅢB) (37.05%). According to Cox multivariate analysis, age, tumor grade, tumor size, operation mode, radiotherapy, chemotherapy, AJCC 8th TNM specific subtype and T stage were independent related factors affecting the prognosis of stage Ⅲ GBC patients (P < 0.01). Conclusion The overall survival of stage ⅢB GBC is better than that of stage ⅢA. The risk of stage Ⅲ GBC death was T1-2N1M0 (ⅢB) < T3N0M0 (ⅢA) < T3N1M0 (ⅢB). Radical cholecystectomy (number of dissected lymph node≥6), radiotherapy and chemotherapy are beneficial to the improvement of prognosis of stage Ⅲ GBC patients.

Long noncoding RNAs (LncRNAs) play critical roles in the development and progression of cancers. However, little is known about the function and mechanism of lncRNAs in non-small cell lung cancer (NSCLC). In this study, we investigated the expression and functional role of lncRNA small nucleolar RNA host gene 20 (SNHG20) as well as its underlying mechanism in NSCLC. Our results showed that SNHG20 was significantly up-regulated in NSCLC tissues and cells. High SNHG20 expression was implicated with poor prognosis in NSCLC patients. Moreover, SNHG20 knockdown suppressed proliferation, migration and invasion, and induced apoptosis in NSCLC cells. Furthermore, SNHG20 could function as a competing endogenous RNA (ceRNA) to elevate ZEB2 and RUNX2 expression by sponging miR-154. Rescue assays revealed that miR-154 inhibition could reverse the inhibitory effect of SNHG20 silence on proliferation, migration and invasion in NSCLC cells. More importantly, SNHG20 knockdown suppressed tumor growth in NSCLC in vivo through suppressing miR-154 and elevating ZEB2 and RUNX2 expression. In summary, knockdown of lncRNA SNHG20 suppressed proliferation, migration and invasion, and promotes apoptosis through up-regulating ZEB2 and RUNX2 expression by sponging miR-154 in NSCLC, providing a promising therapeutic target for NSCLC patients.

Sarin (GB) and soman (GD) are severely toxic nerve agents that react slowly in water, resulting in long-term poisoning of the water and a serious threat to personnel. Some ions can catalyse GB and GD hydrolysis in water; the relevant research for GB is detailed, whereas that for GD is relatively less so. In this paper, GD hydrolysis catalysed by hypochlorite (ClO−) ions was studied via kinetic experiments. A fluorite-ion-specific electrode was used to monitor F− ions produced, allowing the rate constant and half-life of the GD hydrolysis to be calculated. The results showed that ClO− ions promote GD hydrolysis well; the higher the concentration of ClO−, the faster the GD was hydrolysed. In NaClO solution at pH 8.0 with 3.22×10–3 M ClO− ions, the half-life of GD hydrolysis was 82.5 s, about 875 times shorter than that in water at pH 8.0. The rate constant for catalysis of GD hydrolysis by ClO- ions ++(kc1o−)++ was 2.6 M−1 s−1, about one quarter the value of ++koh− ++but over 1500 times greater than kB and ++kPO4,++ with B representing N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid present as a free base; this result indicated that ClO− ions catalyse GD hydrolysis well.

Peroxides like sodium percarbonate (SPC) and sodium percarbonate-acetylsalicylic acid (SPC-Aspirin) can be used to destroy chemical warfare agents (CWAs) such as HD, VX and GD due to the former's oxidation capacity and nucleophilicity. In this paper, experiments were performed to study factors affecting CWAs recovery from peroxides decontaminant water (decon water) and develop the method to recover residual CWAs from peroxides decon water before GC analysis. Results showed that extraction solvent and neutralization were important for high CWA recovery. DCM was more suitable than PE as extractant for most samples except SPC decon water containing HD. Sodium sulfite could well neutralized decontaminant reactivity in decon water. When suitable conditions of simultaneous neutralization and extraction were carried, CWAs recovery from SPC decon water were greater than 90% at a concentration range of 10 mg l-1 to 10000 mg l-1, the recovery of HD, VX and GD from SPC-Aspirin decon water was more than 90%, 80% and 95% respectively at a concentration range of 100 mg l-1 to 10000 mg l-1, while CWAs recovery were relatively lower from SPC-Aspirin decon water with CWAs concentration of 10 mg l-1 due to the degradation of CWAs during pretreatment processes.

The decontaminant activated by MoO42- (MoO42--H2O2) suitable for subzero environment shows strong oxidizing ability and weak nucleophilicity due to its acid. In this paper, in order to improve nucleophilicity and retain oxidation of MoO42--H2O2 as far as possible, NH3 and NaOH were used as alkaline modifiers, and PhSMe was used as a simulant of HD to study the oxidation rate and products of sulfides by alkaline-modified MoO42--H2O2 below zero. Results showed that the reaction rate constants decreased with the increase of pH in both NH3 and NaOH modified MoO42--H2O2 at -20°C, and the relative ratio of sulfone to sulfoxide increased especially at pH>9. The reaction activation energy Ea of PhSMe oxidation in the alkaline-modified MoO42--H2O2 decontaminants was lower than that in the MoO42--H2O2 decontaminant, which indicated that the sensitivity of the oxidation reaction rate to temperature in MoO42--H2O2 was reduced after modification.

Organophosphate neurotoxic agents like Sarin (GB) and Soman (GD) are lethal to person. Except various kinds of decontaminants, they can be also decomposed in natural environment through nucleophilic reaction, where acidic or alkaline substance was to accelerate their hydrolysis. Most of the papers were about GB hydrolysis. Information on GD hydrolysis was relatively small, especially about kinetics of GD in acidic solution. In view of possible effect of positive ion and negative ion on hydrolysis reaction, a relatively simple composes solution, HCl aqueous solutions, was selected to investigate the factors affecting GD hydrolysis rate. Results showed that GD hydrolysis was accorded with the first-order kinetics equation if pH value was kept constant. Its rate constant was independent of GD initial concentration when the amount of H+ was excess than its requested amount. The apparent hydrolysis rate constant (kobs) in pH of 0.90 was about 0.202 min-1 at 20°C, no matter what initial concentration of GD was. The concentration of H+ was the most important factor affecting its rate. The rate constant (kobs) in HCl aqueous as a function of pH value (0.90~2.80) obeyed an equation in 25°C, that is kobs =0.17×10-0.82×pH. Reaction temperature had an obvious effect on hydrolysis rate of GD. Every 10°C increase in temperature, kobs of GD hydrolysis was improved about 2.5 times. The activation energy value (Ea) of GD hydrolysis in HCl aqueous with a pH value of 0.90 was approximately 64.25 kJ/mol.

Hematopoietic stem and progenitor cells (HSPCs) are cells mainly present in the bone marrow and capable of forming mature blood cells. However, the epigenetic mechanisms governing the homeostasis of HSPCs remain elusive. Here, we demonstrate an important role for histone deacetylase 6 (HDAC6) in regulating this process. Our data show that the percentage of HSPCs in Hdac6 knockout mice is lower than in wild-type mice due to decreased HSPC proliferation. HDAC6 interacts with isocitrate dehydrogenase 1 (IDH1) and deacetylates IDH1 at lysine 233. The deacetylation of IDH1 inhibits its catalytic activity and thereby decreases the 5-hydroxymethylcytosine level of ten-eleven translocation 2 (TET2) target genes, changing gene expression patterns to promote the proliferation of HSPCs. These findings uncover a role for HDAC6 and IDH1 in regulating the homeostasis of HSPCs and may have implications for the treatment of hematological diseases.