Search

Your search keyword '"Zhu, Meng-Yang"' showing total 183 results
Start Over Author "Zhu, Meng-Yang"
183 results on '"Zhu, Meng-Yang"'

17. Restoration of Noradrenergic Function in Parkinson’s Disease Model Mice

Author

Cui, Kui, primary, Yang, Fan, additional, Tufan, Turan, additional, Raza, Muhammad U., additional, Zhan, Yanqiang, additional, Fan, Yan, additional, Zeng, Fei, additional, Brown, Russell W., additional, Price, Jennifer B., additional, Jones, Thomas C., additional, Miller, Gary W., additional, and Zhu, Meng-Yang, additional

Published
2021
Full Text
View/download PDF

40. Effects of Chronic Social Defeat on Expression of Dopamine β-Hydroxylase in Rat Brains

Author

FAN, YAN, CHEN, PING, LI, YING, and ZHU, MENG-YANG

Subjects
Male, Transcription, Genetic, Desipramine, Brain, Adrenalectomy, Dopamine beta-Hydroxylase, Spironolactone, Article, Antidepressive Agents, Rats, Inbred F344, Rats, Up-Regulation, Mifepristone, Hormone Antagonists, Fluoxetine, Animals, Rats, Long-Evans, RNA, Messenger, Social Behavior, Stress, Psychological, Mineralocorticoid Receptor Antagonists
Abstract

It is documented that stress activates the locus coeruleus-norepinephrine system. However, there are far few reports regarding effects of stress on the expression of dopamine β-hydroxylase, a hallmark enzyme of the noradrenergic neuron. In the present study, adult Fischer 344 rats were subjected to chronic social defeat for 4 weeks. Dopamine β-hydroxylase expressional levels in the locus coeruleus and its terminal regions were measured by in situ hybridization and western blotting. The results showed that immediately following chronic social defeat there are significantly increased mRNA and protein levels of dopamine β-hydroxylase in the locus coeruleus, and dopamine β-hydroxylase protein levels in the hippocampus, frontal cortex and amygdala, compared with those in the control. This chronic social defeat-induced upregulation of dopamine β-hydroxylase was completely abolished by adrenalectomy, and/or by treatment with corticosteroid receptor antagonists, mifepristone and spironolactone, either alone or in combination. Furthermore, treatment with desipramine, an antidepressant with specific inhibitory effects on norepinephrine transport, prevented an increased dopamine β-hydroxylase expression by chronic social defeat in the locus coeruleus and its main terminal regions such as the hippocampus, frontal cortex and amygdala. However, treatment with fluoxetine, an antidepressant with specific inhibition for serotonin transport, only selectively blocked increased dopamine β-hydroxylase protein levels in the hippocampus caused by CSD. The present findings indicate that chronic social defeat activates the locus coeruleus-norepinephrine system by upregulating the expression of dopamine β-hydroxylase, which may increase norepinephrine synthesis. This chronic social defeat induced upregulation of DBH expression was mediated through corticosterone and corticosteroid receptors, with possible interference from antidepressants.

Published
2013

45. Micro RNAs 29b and 181a down-regulate the expression of the norepinephrine transporter and glucocorticoid receptors in PC12 cells.

Author

Deng, Maoxian, Tufan, Turan, Raza, Muhammad U., Jones, Thomas C., and Zhu, Meng‐Yang

Subjects
MICRORNA, NORADRENALINE regulation, DOWNREGULATION, GLUCOCORTICOID receptors, PROGRAMMED cell death 1 receptors
Abstract

Micro RNAs are short non-coding RNAs that provide global regulation of gene expression at the post-transcriptional level. Such regulation has been found to play a role in stress-induced epigenetic responses in the brain. The norepinephrine transporter ( NET) and glucocorticoid receptors are closely related to the homeostatic integration and regulation after stress. Our previous studies demonstrated that NET mRNA and protein levels in rats are regulated by chronic stress and by administration of corticosterone, which is mediated through glucocorticoid receptors. Whether mi RNAs are intermediaries in the regulation of these proteins remains to be elucidated. This study was undertaken to determine possible regulatory effects of mi RNAs on the expression of NET and glucocorticoid receptors in the noradrenergic neuronal cell line. Using computational target prediction, we identified several candidate mi RNAs potentially targeting NET and glucocorticoid receptors. Western blot results showed that over-expression of miR-181a and miR-29b significantly repressed protein levels of NET, which is accompanied by a reduced [3H] norepinephrine uptake, and glucocorticoid receptors in PC12 cells. Luciferase reporter assays verified that both miR-181a and miR-29b bind the 3′ UTR of mRNA of NET and glucocorticoid receptors. Furthermore, exposure of PC12 cells to corticosterone markedly reduced the endogenous levels of miR-29b, which was not reversed by the application of glucocorticoid receptor antagonist mifepristone. These observations indicate that miR-181a and miR-29b can function as the negative regulators of NET and glucocorticoid receptor translation in vitro. This regulatory effect may be related to stress-induced up-regulation of the noradrenergic phenotype, a phenomenon observed in stress models and depressive patients. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results