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Nasal septal cartilages (NSCs) and mandibular condyle cartilages (MCCs) are two important cartilages for craniomaxillofacial development. However, the role of FLRT2 in the formation of NSCs and MCCs remains undiscovered. NSCs and MCCs were used for immunocytochemistry staining of collagen II, toluidine blue staining, and alcian blue staining. Quantitative reverse transcription‑PCR and western blot were used to detect mRNA and protein expressions of FLRT2, N-cadherin, collagen II, aggrecan, and SOX9. Cell proliferation of MCCs and NSCs was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell counting kit‑8 assay. Cell migration of MCCs and NSCs was examined by wound healing assay and Transwell. Chondrogenesis of MCCs and NSCs were similar in morphological characteristics, while different in cell proliferation, migration, and extracellular matrix. FLRT2 promotes the proliferation and migration of NSCs. There were up-regulation of N-cadherin and down-regulation of collagen II, aggrecan, and SOX9 in NSC with knock down FLRT2. The current study, as demonstrated by Xie et al., reveals that FLRT2 overexpression in Sprague-Dawley neonatal rats promotes the proliferation and migration of NSCs and MCCs, decreases N-cadherin while increases collagen II, aggrecan, and SOX9 in NSC and MCCs. Altogether, FLRT2 mediates chondrogenesis of NSCs and MCCs.

Byline: Qian. Jiang, Yifei. Chen, Fangyong. Zhu, Yujia. Xie, Zhidan. Zhu, Xiaojing. Sun OBJECTIVES: Patients with condylar fractures were treated with osteopeptide injections as an adjuvant therapy to assess pain [...]

The viscoelastic properties of food materials will change significantly while drying is in progress, which greatly influences the food deformation caused by drying. This study aims to predict the viscoelastic mechanical behavior of Hami melon during drying using the fractional derivative model. To characterize the relaxation characteristics, based on the finite difference method, an improved Grünwald–Letnikov fractional stress relaxation model is proposed to derive an approximate discrete numerical solution of the relaxation modulus by applying the time fractional calculus. The Laplace transform method is used to verify the obtained results, and the equivalence of the two methods is proved. In addition, the stress relaxation tests prove that the fractional derivative model has a better prediction effect on the stress relaxation behavior of viscoelastic food than classical Zener model. The significant correlations between the fractional order and the stiffness coefficient and the moisture content is also studied. Which is negative correlation and positive correlation respectively. [ABSTRACT FROM AUTHOR]