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Simultaneous thermogravimetric-mass spectrometric (TG-MS) analyzer was used to investigate the pyrolysis characteristics and gas generation rule of three coal samples. Results indicated that Jing-mei (JM), Xinjiang-mei (XM), and Yan-mei (YM) coals had similar pyrolysis characteristics and gas generation law. Their pyrolysis process could be roughly divided into three stages. YM had the highest weight loss and gas generation rates among all the samples. CO and CO _2 components in the escaping gas continued to increase when the temperature reached 800 °C. Coats–Redfern method was employed to calculate the activation energy of three kinds of coal at four temperature stages with different mode functions. The activation energy of the four stages of JM pyrolysis was 33.3 kJ mol ^−1 , 2.66kJ mol ^−1 , 88.51kJ mol ^−1 , and 33.26 kJ mol ^−1 . The result of XM pyrolysis was 51.29 kJ mol ^−1 , 6.84 kJ mol ^−1 , 47.38 kJ mol ^−1 , and 15.65 kJ mol ^−1 . Calculations of XM pyrolysis were 51.35 kJ mol ^−1 , 5.11 kJ mol ^−1 , and 117.49 kJ mol ^−1 , 31.15 kJ mol ^−1 respectively.

Byline: Hong-bin Deng, Jin-hua Wang, Pei-zhi Liu, Cheng-lin Zhou, Jiang-Feng Wu This paper investigates the simulation design of unmanned helicopter with MATLAB, and completes the development of modelling, control system, navigation system, device input system and virtual reality system, eventually accomplishes the simulation system which can alternate between manned mode and unmanned mode freely. According to the flight test, the simulation and test fit well, this indicates the simulation system accurately describes the characteristics of helicopter dynamics and control system.

Aim: To explore the infiltration pathogenesis of CD4(+);T cells following the spinal nerve ligation., Methods: Healthy adult male SD rats were randomly divided into the spinal nerve ligation group (Tx), sham operation group (S), control group (C). the 50& mechanical paw withdrawal threshold ( 50&MWT ) was determined by up-down method; CD4(+);T cells infiltration was assessed by FACS; the mRNA levels of CCL2, CCL5 and CXCL10 were quantitated by RT-qPCR; serum cytokines were tested by ELISA kits., Results: After 3 days since operation, 50&MWT of Tx group was significantly reduced (P<0.01) comparing with S group, C group; on day 14, 50&MWT was up to the minimum value; whereas S group and C group were no difference (P>0.05). After 7 days since operation, CD4(+);T cells infiltration into lumbar segments of the spinal cord in the Tx group increased significantly (P<0.01), and the CCL2, CCL5mRNA expression increased (P<0.05); on day 14, the CD4(+);T cells infiltration in Tx group was higher than S group, C group; but there was no statistical significance. On day 7 and 14 days, serum levels of cytokines were no difference in the three groups., Conclusion: Following spinal nerve ligation, high expression of chemokine promoted peripheral CD4(+);T cells to infiltrate into spinal cord; and the infiltrated CD4(+);T cells maintained the neuropathic pain.

Aim: To transfect Hlx into mouse dendritic cell line DC2.4 and observe the effect of hlx on function of dendritic cells., Methods: The eukaryotic expression vector PIRES2-EGFP/Hlx was transfected into DC2.4 by liposomes. The transfection efficiency was identified through FACS. RT-PCR and Real-time PCR were used to test the transcription level of Hlx in DC2.4. Forty-eight hours after transfection, DC2.4 cells were studied for cytokine production, cell phenotype, phagocytosis, unilateral mixed lymphocyte reaction., Results: The pIRES2-EGFP/Hlx vector was transfected into DC2.4 with the transfection efficiency of up to 60%. Highly expressed Hlx in DC2.4 increased the expression of maturation makers including CD80 and CD86, and major histocompatibility complex-II. Functional assay showed that over-expression of Hlx in DC2.4 increased the interleukin-12 transcription and decreased DC endocytosis. The Hlx modified DC2.4 highly expressed IL-10 and TGF-β at the same time. Furthermore, it was shown that in a unilateral mixed lymphocyte reaction model, Hlx modified DC2.4 inhibited proliferation of lymphocytes., Conclusion: Transient over-expression of Hlx in DC2.4 promotes DC2.4 maturation and up-regulates IL-12, IL-10 and TGF-β expression. However, the Hlx modified DC2.4 cells functionally appear as regulatory dendritic cells.

Aim: To express human HMGB1 B box protein and obtain monoclonal antibodies (mAbs) against HMGB1 B box for further study of the function of human HMGB1 protein., Methods: pET28-HMGB1 B box plasmid transfected the DH5α, then expressed. And the extracted protein was purified by protein purification system. BALB/c mice were immunized with recombinant human HMGB1 B box protein. Hybridoma cell lines secreting mAb against human HMGB1 B box protein were screened by ELISA and subcloning approach. The characteristics of these mAbs were identified by ELISA and Western blot., Results: Two hybridoma cell lines (1D2F4E3 and 2D4E3A2) stable secreting specific mAbs were successfully obtained.Western blot exhitited the two mAbs binded specifically to human HMGB1 B box protein. The immunoglobulin (Ig) class of two mAbs belonged to IgG, their titers were 1×10(6);, and the A(450); of mAb1D2F4E3, 2D4E3A2 were 0.324±0.093, 0.296±0.085, respectively., Conclusion: Two of high specificity mAbs against human HMGB1 B box protein have been successfully prepared, which laid the foundation for further study of biological function of human HMGB1 protein.

The title compound, [Ag(C(27)H(23)BrN(3)O(4))(CH(3)CN)(2)](PF(6))(2), is a mononuclear salt species in which the silver(I) atom is coordinated by one ligand and two acetonitrile mol-ecules and exhibits a distorted T-shaped coordination. The asymmetric unit contains one independent cation and two independent hexa-fluorido-phosphate anions, one of which is disordered over two positions in a 0.756 (11):0.244 (11) ratio. Weak π-π inter-actions between the anthraquinone ring systems [centroid-centroid distance = 3.676 (3) Å], inter-molecular Ag-π inter-actions [Cg⋯Ag = 3.405 Å] and C-H⋯π inter-actions between pairs of adjacent mol-ecules are observed.

Objective: To explore the effects of total saponins of Rubus parviflolius (TSRP) on brain edema and blood brain barrier in rats., Method: The model of local cerebral ischemia was established in rats by reversible inserting a nylon thread into the anterior cerebral artery through the internal carotid artery brain hydrated amount and content change of Evan' s blue (EB) in cortex subjected to 2h middle cererbral artery occlusion (MACO) followed by 6 h, 24 h, 48 h, 72 h reperfusion and effect of TSRP. penetrability of blood brain-barrier (BBB) the index includes brain hydrated amount and penetrability of blood brain-barrier BBB., Result: Com- pared with I/R group. Both brain hydrated amount and the EB content decreased significantly in TSRP groups on the 6 h, 24 h, 48 h, 72 h of reperfusion after 2 hour of cerebral ischemia induced by MACO model., Conclusion: TSRP could decrease brain hydrated amount and markedly lower permeability of blood-brain barrier subjected to 2 h MACO followed by 24 h reperfusion, and this may be a mechanism of TSRP alleviating brain edema during I/R.