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2. Metabolomic profiling of human pluripotent stem cell differentiation into lung progenitors.

Author

Leibel, Sandra, Tseu, Irene, Zhou, Anson, Hodges, Andrew, Yin, Jun, Bilodeau, Claudia, Goltsis, Olivia, and Post, Martin

Subjects
Cell biology, Metabolomics, Stem cells research
Abstract

Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation toward a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild-type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification toward lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.

Published
2022

8. QS51. Guiding the Way: The Use of a Bioengineered Conduit Reduces Neuroma Formation and Associated Pain Behaviors

Author

Lee, Erica, primary, Enzmann, Bruce, additional, Harris, Thomas, additional, Wong, Alison, additional, Pinni, Sai, additional, von Guionneau, Nicholas, additional, Kothari, Ruchita, additional, Lan, Michael, additional, Qiu, Chenhu, additional, Zhou, Anson, additional, Shores, Jaimie, additional, Latremoliere, Alban, additional, Qu, Lintao, additional, Hoke, Ahmet, additional, Mao, Hai-Quan, additional, and Tuffaha, Sami, additional

Published
2022
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9. A Study of Postoperative Complications Occurring at Home With Pediatric Gastrostomy Feeding Tubes

Author

Aedla, Mihika, primary, Zhou, Anson, additional, Sompel, Kevin, additional, Hu, Katherine, additional, Cheng, Charlotte, additional, Hsu, Jocelyn, additional, Qian, Jason, additional, Zhang, Siya, additional, Ho, Anthony, additional, Slagle, Jacob, additional, Logsdon, Elizabeth A., additional, Sharma, Karun V., additional, and Weiss, Clifford R., additional

Published
2022
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10. P18. A NOVEL BIOENGINEERED CONDUIT REDUCES NEUROMA FORMATION AFTER TARGETED MUSCLE REINNERVATION

Author

Lee, Erica, primary, Enzmann, Bruce, additional, Harris, Thomas, additional, Wong, Alison, additional, Pinni, Sai, additional, von Guionneau, Nicholas, additional, Kothari, Ruchita, additional, Lan, Michael, additional, Qiu, Chenhu, additional, Zhou, Anson, additional, Lee, Alyssa, additional, Shores, Jaimie, additional, Latremoliere, Alban, additional, Qu, Lintao, additional, Hoke, Ahmet, additional, Mao, Hai-Quan, additional, and Tuffaha, Sami, additional

Published
2022
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11. Improving Nerve Coaptation Outcomes in Targeted Muscle Reinnervation: A Novel Bioengineered Device

Author

Lee, Erica, primary, Wong, Alison L., additional, Pinni, Sai, additional, Von Guionneau, Nicholas, additional, Harris, Thomas G.W., additional, Kothari, Ruchita, additional, Lan, Michael, additional, Enzmann, Bruce, additional, Qiu, Chenhu, additional, Zhou, Anson, additional, Shores, Jaimie, additional, Latremoliere, Alban, additional, Höke, Ahmet, additional, Mao, Hai-Quan, additional, and Tuffaha, Sami, additional

Published
2021
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