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Purpose The abnormal function and survival of chondrocytes result in articular cartilage failure, which may accelerate the onset and development of osteoarthritis (OA). This study is aimed to investigate the role of LINC01094 in chondrocyte apoptosis. Methods The viability and apoptosis of lipopolysaccharide (LPS)-induced chondrocytes were evaluated through CCK-8 assay and flow cytometry analysis, respectively. The expression levels of LINC01094, miR-577 and MTF1 were detected by qRT-PCR. Dual luciferase reporter tests were implemented for the verification of targeted relationships among them. Western blotting was employed to measure the levels of pro-apoptotic proteins (Caspase3 and Caspase9). Results The viability of LPS-induced chondrocytes was overtly promoted by loss of LINC01094 or miR-577 upregulation, but could be repressed via MTF1 overexpression. The opposite results were observed in apoptosis rate and the levels of Caspase3 and Caspase9. LINC01094 directly bound to miR-577, while MTF1 was verified to be modulated by miR-577. Both LINC01094 and MTF1 were at high levels, whereas miR-577 was at low level in OA synovial fluid and LPS-induced chondrocytes. Furthermore, the highly expressed miR-577 abolished the influences of MTF1 overexpression on LPS-induced chondrocytes. Conclusions Silencing of LINC01094 represses the apoptosis of chondrocytes through upregulating miR-577 expression and downregulating MTF1 levels, providing a preliminary insight for the treatment of OA in the future.

Abstract Background Osteoarthritis (OA), characterized by inflammation and articular cartilage degradation, is a prevalent arthritis among geriatric population. This paper was to scrutinize the novel mechanism of long noncoding RNA (lncRNA) NEAT1 in OA etiology. Methods A total of 10 OA patients and 10 normal individuals was included in this study. Cell model of OA was built in human normal chondrocytes induced by lipopolysaccharide (LPS). An OA Wistar rat model was established through intra-articular injection of L-cysteine and papain mixtures (proportion at 1:2) into the right knee. Quantitative reverse transcription-polymerase chain reaction was employed to ascertain the expression levels of NEAT1, microRNA (miR)-374b-5p and post-GPI attachment to protein 1 (PGAP1), while dual-luciferase reporter experiments were used for the validation of target relationship among them. Cell cycle and apoptosis were calculated by flow cytometry analysis. CCK-8 assay was done to evaluate the proliferative potentials of chondrocytes. The levels of cell cycle-related proteins (Cyclin A1, Cyclin B1 and Cyclin D2) and pro-apoptotic proteins (Caspase3 and Caspase9) were measured by western blotting. Tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and IL-6 levels were determined via ELISA. Hematoxylin & eosin (HE) Staining was used for pathological examination in OA rats. Results Pronounced downregulation of NEAT1 and PGAP1 and high amounts of miR-374b-5p were identified in OA patients, LPS-induced chondrocytes and OA rats. NEAT1 targeted miR-374b-5p to control PGAP1 expression. Loss of NEAT1 or upregulation of miR-374b-5p dramatically accelerated apoptosis, led to the G1/S arrest and promoted the secretion of inflammatory cytokines in LPS-induced chondrocytes, while ectopic expression of PGAP1 exhibited the opposite influences on chondrocytes. Additionally, we further indicated that upregulation of miR-374b-5p attenuated the effects of PGAP1 overexpression on LPS-induced chondrocytes. Conclusions Reduced NEAT1 induces the development of OA via miR-374b-5p/PGAP1 pathway. This suggests that the regulatory axis NEAT1/miR-374b-5p/PGAP1 is a novel and prospective target for OA treatment.

A Cu–10Al–6Mn–3Fe–4Ga (wt.%) single crystal was simply prepared, and its microstructure, reversible martensitic transformation, mechanical and SME properties were investigated. The results show that this single crystal consists of dominant 2H martensite retained L21 parent and fine Fe-rich nanoparticles. Its reversible martensitic transformation temperatures are respectively 313 K (Ms), 299 K (Mf), 339 K (As), and 354 K (Af), which are suitable for practical applications. A large stress platform with a strain of approximately 8% was associated with the reorientation of existing martensite and stabilization of stress-induced martensite. Furthermore, full shape recovery characteristics are obtained all the time while changing the pre-strains from 6% to 11%. The maximum shape memory effect is up to 9.2% with a 100% shape recovery rate.

Giant axonal neuropathy (GAN) is a very rare autosomal recessive disorder characterized by abnormally large and dysfunctional neuronal axons. Mutations in the GAN gene have been identified as the cause of this disorder. In this report, we performed a detailed phenotypic assessment of a Chinese patient with GAN. An array-based exon capture test and targeted next-generation sequencing were used to detect the suspected mutation sites. Compound heterozygous mutations of p.S79L (c.236C > T) in the BTB domain and p.T489S (c.1466C > G) in the kelch domain were identified in the proband’s genome. S79L was a known mutation, and T489S was reported for the first time. The p.S79L and p.T489S were confirmed in the proband’s mother and father, respectively. Both mutations were located in highly conserved regions and affected the predicted protein crystal structures. The proband’s sural biopsy revealed the classical GAN phenotype of swollen axons filled with closely packed neurofilaments. The combined application of the next-generation sequencing platform and bioinformatics analyses was an effective method for diagnosing GAN. The novel compound mutations of S79L and T489S in the GAN gene were likely the cause of the patient’s GAN symptoms. Our findings enrich the spectrum of mutations associated with this rare type of axonopathy.

The pilot well BYP5 is a cored well drilled to explore the oil and gas bearing properties of the highly thermally evolved lower sub-member of the third member of the Paleogene Shahejie Formation in the Bonan deep sag of the Zhanhua Sag, Bohai Bay Basin. The coring interval depth ranges from 4 267.0 to 4 338.1 m. To reveal the oil and gas bearing properties of the cored interval, pyrolysis of frozen, sealed fragments was conducted on typical samples and the escaping gas from the core was collected and quantified for composition analysis. The results show that the cored interval is a high-quality hydrocarbon source rock rich in organic matter and carbonates, with a maturity (Ro) of about 1.2%. Efficient hydrocarbon generation and expulsion likely occurred during thermal evolution, causing the current low free hydrocarbon (S1) and hydrogen index (IH) values. The content of the hydrocarbon gas from the core was generally low, ranging from 0.001 to 0.01 cm3/g, with an average of 0.005 cm3/g. Segments with relatively high levels of escaping hydrocarbon gas corresponded to those with relatively high pyrolysis S1 values. The escaping gas was mainly composed of CH4, CO2, H2, and C2H6, with mole percentages of H2 ranging from 1.08% to 19.23%, with an average of 7.09%, indicating hydrogen-rich characteristics. H2 showed a significant positive correlation with CO2 and a negative correlation with CH4. The escaping gas from the core was likely trapped in-situ, and the formation of H2 might be related to the cleavage of hetero-bonds and demethylation during the pyrolysis of organic matter. Further research is suggested on the formation mechanism, geological exploration, and evaluation of natural hydrogen released during organic matter pyrolysis, so as to provide a basis for the decision-making in the exploration and development of this type of natural hydrogen resource.

Spirulina is capable of using light energy and fixing carbon dioxide to synthesize a spectrum of organic substances, including proteins, polysaccharides, and unsaturated fatty acids, making it one of the most coveted food resources for humanity. Conventionally, Spirulina products are formulated into algal powder tablets or capsules. However, the processing and preparation of these products, involving screw pump feeding, extrusion, high-speed automation, and high-temperature dewatering, often result in the rupture of cell filaments, cell fragmentation, and the unfortunate loss of vital nutrients. In contrast, fresh Spirulina, cultivated within a closed photobioreactor and transformed into an edible delight through harvesting, washing, filtering, and sterilizing, presents a refreshing taste and odor. It is gradually earning acceptance as a novel health food among the general public. This review delves into the manufacturing processes of fresh Spirulina, analyzes its nutritional advantages over conventional algal powder, and ultimately prospects the avenues for fresh Spirulina’s application in modern food processing. The aim is to provide valuable references for the research and development of new microalgal products and to propel the food applications of microalgae forward.

High-quality lacustrine shale beds within the Paleogene Hetaoyuan Formation in the Biyang Sag, Nanxiang Basin, exhibit significant potential for shale oil resources. Previous studies on the oil-bearing potential and hydrocarbon occurrence of shales in the Biyang Sag were relatively limited, potentially hindering advancements for shale oil exploration. This study focuses on the shales found in the third sub-member of the third member within the Hetaoyuan Formation in well Y1, which locates in the southern area of the Biyang Sag. Through various geochemical analyses such as Rock-Eval pyrolysis, multi-temperature pyrolysis and X-ray diffraction, this research systematically investigates the shale oil contents, hydrocarbon occurrence characteristics, and influencing factors. These results indicate that the lithofacies assemblages of the shale primarily consist of felsic shale facies, dolomitic shale facies and mixed shale facies, with a well-developed laminar structure. The source rock quality is classified as good to excellent, and the thermal maturity stage of oil window. The predominant organic maceral is sapropelic, with organic matter types ranging from Ⅰ to Ⅱ1. Oil content increases with burial depth, ranged from adsorbed hydrocarbons in the upper section to free hydrocarbons in the lower section. Detrital mineral content and total organic carbon (TOC) content are identified as the main factors influencing free and adsorbed hydrocarbon content, respectively. Overall, the oil saturation index (OSI) of the shale in the lower section of the Hetaoyuan Formation exceeds 100 mg/g, with an average free hydrocarbon content over 3 mg/g, indicating promising prospects for shale oil exploration and development.

Intensity-modulated direct-detection (IM/DD) optical systems are most widely employed in short-reach optical interconnects due to their simple structure and cost-effectiveness. However, IM/DD systems face mixed linear and nonlinear channel impairments, mainly induced by the combination of square-law detection and chromatic dispersion, as well as the utilization of low-cost non-ideal transceivers. To solve this issue, recent years have witnessed a growing trend of introducing machine learning technologies such as neural networks (NNs) into IM/DD systems for channel equalization. NNs usually present better system performance than traditional approaches, and various types of NNs have been investigated. Despite the excellent system performance, the associated high computational complexity is a major drawback that hinders the practical application of NN-based equalizers. This paper focuses on the performance and complexity trade-off of NNs employed in IM/DD systems, presenting a systematic review of the current status of NN-based equalizers as well as a number of effective complexity reduction approaches. The future trends of leveraging advanced NN in IM/DD links are also discussed.

Objective To evaluate the efficacy and safety of plasma-derived human coagulation factor Ⅷ (FⅧ) in the treatment of patients with hemophilia A. Methods A multi-center and open, SAT(single-arm trials) clinical study was conducted. A total of 54 subjects with hemophilia A were enrolled in 5 research centers. FⅧ was injected according to the subjects' weight, severity of disease and other factors, and the transfusion efficiency of FⅧ activity at 10 min after the first infusion of the first bleeding event was taken as the main efficacy indexes. The improvement scores of bleeding symptoms and signs within 24 h after the first infusion of the first bleeding event were the secondary efficacy indexes. The pathogenic microbial indexes and FⅧ inhibitors were detected on 90(th) and 180(th) day after treatment. Results The transfusion efficiency of FⅧ activity of 54 subjects at 10 min after the first infusion was 171.9% on average, with median of 169.5%, both higher than the target value of 100%. Within 24 h after the first infusion, the improvement of bleeding symptoms and signs of the subjects were scored, among which 19 cases (35.2%) were "obvious", 35 cases (64.8%) were "good", and the total clinical effective rate reached 100%. Five subjects (9.3%) had six drug-related adverse events. On 90(th) and 180(th) day after treatment, hepatitis B surface antigen, hepatitis C antibody, HIV antibody, treponema pallidum antibody and FⅧ inhibitors were detected, and no negative to positive cases were found. Conclusion After infusion, the FⅧ preparation can significantly improve the FⅧ activity level in hemophilia A patients in a short period of time, which has high infusion efficiency and can achieve better treatment efficacy, and can also effectively control and relieve bleeding symptoms and signs, with good overall safety.

The Junggar Basin is rich in oil and gas resources, with huge exploration potential, which is one of the main arenas of increasing oil and gas reserve and producing in China. In recent years, CNPC has made major breakthroughs in exploration in the Junggar Basin, revealing the orderly symbionic distribution pattern of conventional and conventional oil and gas. In order to further learn from the oil and gas achievements and exploration and development experience in neighboring areas and the new geological theories, for formulating SINOPEC's oil and gas exploration strategy in the Junggar Basin, the potential and distribution characteristics of the oil and gas resources in the Junggar Basin are systematically analyzed in this paper and it is pointed out that no enough attention is paid to natural gas and unconventional petroleum. The key breakthrough direction and exploration strategy of SINOPEC in the Junggar Basin are put forward in the paper after reviewing the major exploration breakthroughs in multiple fields, multiple strata, and multiple types in recent years and combining with the oil and gas geolo-gical conditions and exploration practice of SINOPEC's mining rights area. The research reveals that the Junggar Basin has entered a new period of exploration dominated by the middle and lower assemblage, and the exploration ideas must be adjusted accordingly. We must insist on the transformation from early outside-source exploration to intra-source and near-source exploration, and from early conventional petroleum exploration to both conventional and unconventional petroleum exploration. In order to make big exploration breakthrough of SINOPEC and realize the target of increasing reserves and production in the near and medium term in the Junggar Basin as soon as possible, four exploration strategies are proposed in the paper, including further figuring out the resources, establishing an integrated research team of conventional and unconventional petroleum, strengthening the joint research of geological and engineering integration, and increasing the support of risk exploration.

The geological conditions of Hashan area on the northwestern margin of the Junggar Basin are complicated and it is difficult to explore. It is of great significance to clarify the sedimentary and evolutionary characte-ristics of source rocks in the Permian Fengcheng Formation in this area for expanding oil and gas exploration on the basin margin. Based on the analysis of whole-rock XRD, elemental geochemistry, organic matter abundance, thin section identification, and lithofacies association characteristics, this paper conducted a comparative analysis of the sedimentary characteristics, lithology, and lithofacies association characteristics of the Fengcheng Formation in the Mahu Sag and Hashan area, restored the ancient sedimentary environment evolution sequence in the study area, and established a model of ancient sedimentary evolution. The research results show that the lithofacies association characteristics and ancient sedimentary environment evolution sequence of the Fengcheng Formation in the Hashan area are highly similar to those in the Mahu Sag, which are generally fine-grained sediments mixed from multiple sources in an alkaline lake with a volcanic background, and various lithofacies associations are developed. The sedimentary paleoenvironment has phased evolution characteristics. The large number of typical alkaline minerals in the second member of Fengcheng Formation in Hashan area reveals the development of another lake basin center in addition to the Mahu Sag. During the deposition of the first member of Fengcheng Formation (P1f1), the lake level was relatively higher, the water salinity was low, the climate was semi-arid, and volcanic sedimentation was developed, with lithofacies mainly composed of organic-rich blocky tuffaceous limestone. In the lower part of the second member of Fengcheng Formation (P1f2), the basin began to shrink, the climate became relatively drier, the water became saltier, the environment became more limited, and the lithofacies were mainly organic-rich layered dolomitic mudstone. In the top of the P1f2 and the lower part of the third member of Fengcheng Formation (P1f3), the environment was relatively closed, the water salinity was higher, a large number of alkaline minerals were developed, and the lithofacies were mainly organic-rich layered alkaline dolomitic mudstone and organic-rich layered mixed shale. In the upper part of P1f3, the input of terrigenous debris increased, salinization weakened, a fan-delta system was developed, and the lithofacies were mainly organic-rich blocky fine sandstone. The sedimentary environment of the Fengcheng Formation has a controlling effect on organic matter enrichment. Overall, a deep-water environment with less input of debris, warm and humid conditions, and relatively lower salinity is more conducive to organic matter enrichment.

The Shawan Sag is one of the important exploration areas in the hinterland of the Junggar Basin. In order to better clarify the source of crude oil from the Permian, Jurassic and Cretaceous reservoirs in the sag and identify hydrocarbon accumulation processes and enrichment pattern, the authors conducted a carbon isotope and biomarker analysis on the typical crude oil samples from the eastern Shawan Sag in this study, and further carried out crude oil classification and oil-source correlation based on the analysis of geochemical characteristics of crude oil. The results indicate that the crude oil from different strata in the eastern Shawan Sag can be divided into three types. The first type mainly occurs in the Permian and Jurassic reservoirs, and its whole oil δ13C value and Pr/Ph ratio range from -31.0‰ to -29.0‰ and from 1.0 to 2.0, respectively. The relative contents of αααR regular sterane display the characteristics of C27 < C28 < C29, and the terpanes are mainly characterized by C20 < C21>C23 TT and C24TeT/C26TT < 1, with the gammacerane index lower than 0.30. This type of crude oil is mainly derived from the source rocks in the Middle Permian Lower Wuerhe Formation. In the oil samples from the Upper Permian Upper Wuerhe Formation, Ts content is below the detection limit, the β-carotane/C30 hopane ratio is greater than 1, and the tricyclic terpane distribution displays the characteristics of C20>C21>C23TT. The distribution fraction of methyl phenanthrene reflects that the crude oil is in the stage of high-maturity evolution. All these indicate the mixing of crude oil generated in the Lower Permian source rocks. The second type occurs in the Middle Jurassic reservoirs. The whole oil δ13C value and individual n-alkanes δ13C value are higher than -29.0‰. The Pr/Ph ratio is relatively higher, ranging from 2.0 to 2.5, with C24TeT/C26TT>1, which indicate that this type of crude oil is mainly derived from the Jurassic source rocks. The third type occurs in the Lower Cretaceous reservoirs, and its whole oil δ13C value and Pr/Ph ratio are lower than -31.0‰ and 1.0, respectively. The αααR regular sterane contents display the characteristics of C27≈C28

Compared with the Mahu Sag in the Junggar Basin, the adjacent Hashan area has undergone multi-stage napping and complicated oil-gas formation and evolution process, making its shale oil exploration facing many challenges, such as unclear dominant lithofacies and enrichment law. In order to optimize the dominant lithofacies of the Lower Permian Fengcheng Formation shale in Hashan area and summarize the enrichment model of shale oil, by taking more than 200 samples from 8 exploration wells in the area, including Hashan 5 and Hashen X1 wells, and using methods such as core and ordinary thin section observation, a systematic classification of shale lithofacies types was carried out based on the study of sedimentary environment and facies. A comprehensive evaluation of reservoir physical properties and oil content was conducted based on lithofacies by combining experimental methods such as XRD, scanning electron microscopy, low-temperature nitrogen adsorption, and pyrolysis. The enrichment law of the Fengcheng Formation shale oil in Hashan area was preliminarily revealed based on the correlation analysis of shale mineral composition, organic matter abundance and oil content. The results show that the Fengcheng Formation shale in Hashan area can be divided into four lithofacies types: terrigenous clastic, dolomitic diamict, alkaline mineral diamict and volcanic clastic diamict. The (argillaceous) siltstone in terrigenous clastic lithofacies, the dolomitic sandstone in dolomitic diamict lithofacies, and the volcanic clastic diamict lithofacies have good physical properties and oil content, which are good shale oil reservoirs. The shale oil enrichment patterns can be divided into three types: in the terrigenous clastic lithofacies of the outer delta front, the shale oil enrichment patterns are mainly migrated from adjacent strata. In the shallow- semi-deep lake section, the dolomitic mixed-deposit lithofacies are mainly the shale oil enrichment model of the same layer fracture migration and adjacent layer migration, while the volcaniclastic mixed-deposit lithofacies are mainly the self-generated and self-stored matrix shale oil enrichment model.

Abstract Today's world faces the dual pressure of carbon dioxide (CO2) emission reduction and an energy crisis. Microalgae, which can use solar energy to convert CO2 to organic matter, have emerged as a promising and renewable cell factory for producing nutrients, biofuels, and various high value‐added compounds (HVACs). They possess numerous advantages, such as high photosynthetic efficiency, fast growth rate, and use of agro‐industrial waste and nonagricultural land for cultivation. Microalgae can also effectively remove eutrophic elements (e.g., nitrogen and phosphorus) from wastewater and atmospheric pollutants (e.g., SOx and NOx) from flue gas, thus providing great environmental benefits. However, microalgae‐based production often faces low productivity, limiting applicability in industrial settings. Genetic and metabolic modifications of certain microalgal strains have proven effective in improving productivity. Here, we review the latest developments regarding the microalgae‐based production of platform compounds, biofuels, and other HVACs. Although still in the early exploration stage, the rapid development of gene editing tools, a deeper understanding of the metabolic pathways of microalgae and their regulatory mechanisms, and further optimization of cultivation procedures and photosynthetic efficiency can eventually enable the launch of microalgae‐based biomanufacturing for green industrial production. Therefore, this technology is strategically important for solving the current energy crisis problems of excessive CO2 emissions and environmental pollution. This review provides information about the advancement and development of microalgae‐based production over the past two decades and discusses possible future directions in the field.

In order to evaluate the shale oil prospecting potential of the Mesoproterozoic Hongshuizhuang Formation in the Jibei Depression, the analyses of routine Rock-Eval pyrolysis, matrix porosity and permeability, mercury intrusion and nitrogen adsorption, argon ion polishing SEM and X-ray diffraction of bulk rock of typical core samples were carried out in this study. The shale oil forming conditions of the Hongshuizhuang Formation were studied, the shale oil resource was assessed, and the prospecting potential was discussed based on previous results. The results show that the lagoon facies black shale in the middle section of the Hongshuizhuang Formation is mainly 35-55 m thick with organic matter types of Ⅱ1 and Ⅱ2, and is a set of good- to excellent-quality source rock. The equivalent vitrinite reflectance of organic-rich black shale is 0.80%-1.25%. The matrix porosity is mainly 1.0%-8.0%, and the matrix permeability is (2-100)×10-6 μm2. The pores are dominated by inorganic pores and fissures with the diameter of pore larger than 50 nm. The values of S1 after recovery is mainly 1.60-6.50 mg/g, and the content of residual hydrocarbon gas distributes 0.13-0.24 m3/t, indicating good hydrocarbon potential. The average content of brittle minerals is 61.9%-72.3%, with good frangibility. The shale oil geological resource is about 12×108 t, and the shale oil recoverable resource is about 0.72×108 t for organic matter rich shale of the Hongshuizhuang Formation. These suggested that the organic matter rich shale of the Hongshuizhuang Formation in the Jibei Depression has a resource basis for shale oil exploration with good prospecting potential.

In this article, trellis-coded modulation-enabled probabilistic shaping (TEPS) scheme with a simplified Viterbi decoder is proposed. Trellis-coded modulation (TCM) is embedded in the probabilistic shaping (PS) technique to improve the performance of PS signals in bandwidth-limited intensity modulation and direct detection (IMDD) systems. Further, to reduce the calculation complexity of TCM decoding, the constellation partition decision-assisted (CPDA)-Viterbi decoder is proposed which decides the received signal before decoding to reduce the branches. To verify the performance of the proposed scheme, a 32-GBaud TEPS-four-level pulse-amplitude modulation (PAM4) experimental system with 10G-class O-band directly-modulated laser (DML) is built over a 20-km standard single-mode fiber (SSMF) transmission link. The experimental results show that TEPS-PAM4 achieved up to 1.80-dB higher receiver sensitivity than PS-PAM4 at 7% forward error correction (FEC) limit with the same net rate. Moreover, with the multiplications reduced by up to 45.7% compared with the Viterbi decoder, TEPS-PAM4 with CPDA-Viterbi decoder has up to 1.50-dB higher receiver sensitivity limit than PS-PAM4 at 7% FEC.

Since the development of unconventional oil and gas business, Nuclear Magnetic Resonance (NMR) technology has been gradually applied in the evaluation for unconventional reservoirs due to the merits such as nondestructive, sensitive and fast, this technology has become one of the important methods in shale oil and gas reservoir evaluation. Therefore, based on the experimental principle of NMR technology, this paper focuses on the applications of NMR technology in the full-scale integrated characterization of pore and fracture distribution, characterization of shale porosity, pore wettability, fluid mobility and fluid classification, etc. In addition, the applications of NMR in describing water migration, methane adsorption and desorption, carbon dioxide displacement and other fluid behaviors, obtaining organic matter information, oil shale interface area, determining organic pores and inorganic pores, analyzing pore connectivity, and obtaining information about high-viscosity asphalt and kerogen are also briefly reviewed. Finally, the shortcomings of NMR and the development trend of NMR in shale reservoir evaluation are analyzed.

Como nuevo tipo de contaminante, los microplásticos han estado atrayendo una gran atención durante el proceso de monitorización del medio marino. Las redes de arrastre utilizadas en la actualidad (con un tamaño de malla de 330 μm) no son realmente adecuadas para la recogida de partículas de microplástico más pequeñas debido a su gran tamaño de malla. Además, el método de captura con redes de arrastre requiere de operaciones complicadas, durante las cuales las muestras son susceptibles de contaminación en un entorno abierto. En este contexto, se desarrolló un novedoso sistema de muestreo in situ y de clasificación de microplásticos de agua de mar que integra un procedimiento de recogida, separación y enriquecimiento, y cuenta también con un sistema de elución alternativo aire-agua y de recogida con clasificación de tres capas (con tamaños de malla de membrana de 300, 100 y 50 μm). Entre sus ventajas se incluyen su facilidad de utilización, la reducción en el tiempo de su operación, el reciclado del agua del mar y la formación de un espacio cerrado con el fin de evitar la contaminación de las muestras. Además, es capaz de medir con precisión el volumen de agua de mar filtrada y las pruebas de elución realizadas con él demuestran que su tasa de recuperación alcanza más de un 90 % en cinco segundos. Posteriormente se llevó a cabo un experimento en tierra con el fin de recoger muestras de microplásticos utilizando el nuevo sistema. Las muestras después fueron analizadas e identificadas por espectroscopia micro-Raman, encontrándose microplásticos de diferentes tipos (PE, PET, PA, PEVA, PS y PVS); colores (translúcido, blanco, azul y amarillo), y formas (espuma, fragmentos, fibras y gránulos) en las membranas filtrantes de 100 y 50 μm, pero no en la membrana filtrante de 300 μm. Los resultados del estudio revelan que el nuevo sistema de muestreo resulta realmente eficaz en la recogida de microplásticos que por lo regular es omitida por las redes de arrastre y que puede utilizarse como complemento a dicho sistema.

At present, studies of the shales in Qianjiang Formation from the Qianjiang Depression has been widely documented and significant progress has been made in the aspects of geochemical characteristics and reservoir physical properties.However, the complexity of pores, the main minerals forming pore throats and their influencing factors is still poorly understood. In this work, based on fractal theory and combined with nitrogen adsorption and high-pressure mercury injection experiments, Wells BX7 and BYY2 at different deposition locations were selected to evaluate the specific surface fractal characteristics, pore structure fractal characteristics and pore throat fractal characteristics of the Eq34-10 rhythmic shale of the Qianjiang Formation and analyses the influencing factors.The results show that the pore surface of shale in study are a is relatively flat, with the fractal dimension D1 being close to 2, and the roughness of the pore surface is mainly affected by the characteristics of the clay minerals. Compared with Well BX7 at the sedimentary edge, Well BYY2 at the sedimentary center has simpler fractal characteristics of reservoir pore structure. The larger the proportion of pore volume with a small pore size is, the more complex the fractal characteristics of the pore structure are. The fractal dimension D2 of the reservoir pore structure in Well BX7 is mainly affected by clay minerals and quartz, while dolomiteis the dominant controlling factor for the fractal dimension D2 of the reservoir pore structure in Well BYY2. Dolomite is the main mineral that constitutes the pore throat of the Well BX7 reservoir. With the increase in dolomite, the pore throat features are complex, and the pore connectivity becomes poor. The pore throat diameter formed by quartz in the Well BYY2 reservoir is small.The increase in quartz will worsen the pore connectivity, and the pore throat diameter formed by calcite is large. The occurrence of shale oil will reduce the fractal dimension of pores and have little influence on the fractal characteristics of pore throats. The presence of salt minerals can block the pores and make the pore connectivity poor.

To study the mechanism of Cu toxicity on wheat, the characteristics of Cu stress in pivotal growth periods of wheat were explored by field planting methods. The results showed that at the tillering stage, the concentrations of Cu in the leaf cell fluid were significantly higher than those in the cell wall, and the Cu was primarily enriched in cell fluid. At the jointing and heading stages, the Cu concentration in the leaf cell wall was significantly higher than that in the cell fluid, and the main enrichment was transferred to the cell wall. During the above three growth stages, no Cu was discovered in leaf organelles. Further studies showed that the total soluble protein content in wheat leaves at the tillering and jointing stages showed a trend of first rising and then falling with increased Cu dosage. At the heading stage, under low and medium Cu stress, the total soluble protein content showed no remarkable change. Malondialdehyde (MDA) content at the tillering stage increased with the increase of Cu concentration in the soil, while MDA content did not change noticeably at the jointing and heading stages. At the tillering and heading stages, the low concentrations of Cu increased peroxidase (POD) activity. The POD activity decreased gradually with the increased Cu concentration. However, at the high concentrations of Cu, there was no significant difference in the activity of POD. At the jointing stage, the POD activity did not change significantly under the low Cu stress while it was evidently inhibited under high Cu stress. Based on the above studies, further analyses on the correlation between canopy spectral characteristics and the Cu accumulation at different growth stages of leaf cells were performed, and a new combined index SIPI/NDVI705 performed well in Cu content prediction. The results showed that at different growth stages, different sensitive spectral characteristic parameters should be used to predict the Cu content in leaf cells. [ABSTRACT FROM AUTHOR]

This paper analyzes the typical technical problems arising from dumping and tamping collision interferences in the working faces of conventional mechanized solid backfilling mining (SBM). Additionally, the technical and consecutive characteristics of the solid intelligent backfilling (SIB) method, the execution device, and the corresponding process categories of the SIB process are analyzed. A design for an SIB process flow is presented. Critical algorithms, including automatic recognition and optimization planning based on the cost function and laying the algorithm foundation, are proposed to develop a backfilling process control system. A joint simulation test system is built on a MATLAB/Simulink simulation toolkit (MSST) to simulate and test the optimized algorithms. The results show that the optimized algorithm can realize the automatic optimization planning and automatic interference-recognition adjustment of the backfilling process under actual engineering conditions. In conclusion, this paper analyzes typical technical problems in the conventional backfilling process, designs the SIB process flow, and develops key algorithms to achieve the automatic control of the backfilling process.

How to improve the stability and processability of organic-inorganic hybrid perovskite quantum dots (OHPQD) is an important topic in recent research. In this work, the stability and machinability of OHPQD were improved by blending with PU elastomer. The CsPbBr3−PU composites can be achieved easily by pouring molding process with rather excellent humidity and thermal stability. The tensile strength of 14 ​MPa and elongation of 580% at break of PU/CsPbBr3 elastomer composites with self-repair properties were obtained, which was attributed to the introduction of disulfide bonds. It lays a foundation for the application of OHPQD.

The matrix type shale oil will become the main contributer for increasing shale oil reserves and production in China. Maturity is an important factor for the controlling to the distribution of sweet spot area for matrix type shale oil. The vitrinite reflectance suppression ranges of continental organic rich shale with different organic matter type were analyzed, and the maturity limit of sweet spot area for continental matrix type shale oil was discussed taking the lower Es3 (third member of Shahejie Formation) and upper Es4 (fourth member of Shahejie Formation) sub-members of the Dongying Sag as a case study. The vitrinite reflectance suppression ranges of continental organic rich shale are positively correlated to organic matter types. The actual maturity (EqVRo) of organic matter rich shale of the lower Es3 in subsags of Dongying Sag was mainly at the range of 0.69%-1.05%, and that of the upper Es4 is mainly at the range of 0.74%-1.20%. Combining with the analysis of geological and engineering sweet spot conditions, the EqVRo of organic matter rich shale of both two sub-members range 0.74%-1.20%. The deeper areas of four sub-sags in the Dongying Sag all have favorable prospecting of commercial development for matrix type shale oil in the lower Es3 and upper Es4 sub-members.

Abstract Background This study aimed to investigate the anesthetic effect of butorphanol with different doses in patients undergoing gastroscopy and colonoscopy. Methods 480 patients undergoing gastroscopy and colonoscopy were recruited and randomly divided into four groups to receive different doses of butorphanol (Group A = 2.5 μg/kg, Group B = 5 μg/kg, Group C = 7.5 μg/kg and Group D = 10 μg/kg). Butorphanol was administered 5 min before propofol infusion. The primary outcome was the incidence of body movement. Secondary outcomes were postoperative recovery time, length of stay in the Post-Anesthesia Care Unit (PACU), the total dose of propofol, and the incidence of intraoperative hypoxemia, propofol injection pain, cough, postoperative nausea and vomiting, drowsiness, and dizziness. Results The incidence of body movement and the dose of propofol in Group C and D were lower than those in Group A and B (P

Abstract Background Postoperative cognitive dysfunction is a common postoperative complication in elderly patients. In elderly patients, the decline of organ function and neuromuscular junction function make them more sensitive to muscle relaxants. They are more likely to experience residual muscle relaxation after surgery, which may cause various adverse events. Neostigmine, a commonly used muscle relaxant antagonist, can reduce the expression of inflammatory factors, thereby reducing the pro-inflammatory response and neurodegeneration of the cerebral cortex and hippocampus after surgery. The study aimed at observing the effect of different doses of neostigmine on postoperative cognitive function and peripheral inflammatory factors in elderly patients. Methods One hundred thirty-two elderly patients who underwent a radical section of gastrointestinal cancer at First Affiliated Hospital of Dalian Medical University were divided into neostigmine and saline groups at a 2:1 ratio. Neostigmine was intravenously injected in the post-anesthesia care unit (PACU) according to the train-of-four ratio (TOFR) T4/T1. When TOFR was ≤0.5, 0.04 mg/kg neostigmine was administered, whereas when TOFR was > 0.5, 0.02 mg/kg neostigmine was injected. The main observation indexes were cognitive function, interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) in peripheral blood at the different times before and after the surgery. Secondary observation indicators include the number of atropine injection, extubating time, PACU residence time, incidence of hypoxemia, hypercapnia, and postoperative nausea and vomiting in PACU, time of exhaustion, and length of hospitalization. Results The extubating and PACU times in 0.04 mg/kg and 0.02 mg/kg groups were significantly shorter than those in the control group (P

Pore space containing mobile shale oil was more precisely characterized by comparing the change of adsorption volume of low-temperature nitrogen experiments and the change of mercury intake volume in high-pressure mercury injection experiments and the change of pore diameter using field emission scanning electron microscopy. The Eq34-10 rhythmic mobile hydrocarbon mainly occurs in dolomitic intercrystalline/intergranular pores and clay mineral interbedded pores, and movable hydrocarbon is relatively rich in lamellar shale. Low temperature nitrogen adsorption and high-pressure mercury injection measurements of pore size were spliced with pore size of 80 nm, and the results showed that movable hydrocarbon mainly occurs in the range of pore size less than 200 nm, and also occurs in the micron sized pores. Relatively more movable shale oil occurs in pores from 90 nm to 200 nm. The samples with low clay mineral content can yield movable hydrocarbon within the pores of ≤5 nm. The higher the porosity, the larger the average pore size, and the richer the movable shale oil.

In this paper, a simplified soft-output direct detection faster than Nyquist (SO-DD-FTN) algorithm is proposed to eliminate severe inter-symbol interference in optical interconnects. The feasibility of the proposed algorithm is verified in a C-band 56-Gb/s 4-ary pulse amplitude modulation (PAM-4) system using 10G-class optics, and the 3-dB system bandwidth is only 5.5 GHz. Compared to conventional SO-DD-FTN, the simplified SO-DD-FTN not only reduces 90% multiplication complexity and 80% addition complexity, but also has almost no performance degradation. Experimental results show that, for the back-to-back case, the achieved receiver sensitivity using simplified SO-DD-FTN has 4.5-dB improvement compared with conventional direct detection faster than Nyquist (DD-FTN). As for 20-km standard single-mode fiber (SSMF) transmission application, since the system suffers from both optical/electronic bandwidth limitation and dispersion-induced power fading, the conventional DD-FTN cannot achieve the bit error rate (BER) threshold of 10-3, while the simplified SO-DD-FTN can achieve the BER threshold at received optical power of -14.5 dBm. In conclusion, the proposed simplified SO-DD-FTN algorithm achieves superior performance with low computational complexity and has the potential for applying to optical interconnects.

Due to an increasing energy demand and the depletion of conventional oil, there is ever increasing demand for unconventional shale oil and gas resources. As the most hydrocarbon-rich sag in the Junggar Basin, the development prospects for shale oil in the Mahu Sag have become a focus of research. However, so far, there have been few studies of the oil-bearing properties of shale and shale oil mobility in the sag. This paper redresses this using a range of methods, such as pyrolysis and multi-temperature step pyrolysis. The results show that the Fengcheng Formation shales are generally good quality source rocks. The main body of the shale is low mature-mature, and the type of organic matter is mostly Type II kerogen. In the depth intervals at 4616.45~4640.30 m, 4661.25~4695.20 m, 4728.30~4759.80 m, 4787.60~4812.30 m, and 4876.70~4940.25 m, the oil-bearing properties of the shale and shale oil mobility are good, with an average S1 of more than 1.5 mg/g, OSI of more than 100 mg/g.TOC-1, a ratio of free/adsorbed oil (S1−1+S1−2/S2−1) of more than 3, and a ratio of free/total oil (S1−1+S1−2/S1−1+S1−2+S2−1) of more than 80%. The second member (P1f2) and the lower part of the first member (P1f1) of the formation offer the most promising commercial prospects. Shale oil mobility in the formation is greatly affected by the abundance of organic matter. The higher the TOC value, the greater the hydrocarbon generation capacity, and the better its adsorption capability in the shale. The Fengcheng Formation shale is mature, and shale oil mobility is good. Impacted by the main reservoir space, the felsic shale in the formation has optimal shale oil mobility, with the shale oil being characterized by self-generation and self-storage, and accumulation in adjacent layers.