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Abstract This paper discusses the seismic performance of five reduced-scale shear walls, including one cast-in-place (CIP) concrete shear wall, two precast concrete (PC) shear walls with overlapping U-bar loop connections, and two PC shear walls with modified form-overlapping U-bar loop connections combined with extruded sleeve connections. A quasi-static test was conducted to evaluate the reliability of the overlapping U-bar loop connections and the modified form by comparing the corresponding mechanical parameters of PC specimens with those of the CIP specimen. Moreover, the differences in seismic performance between the CIP specimen and PC specimens with different connection methods were also analyzed in terms of damage process, hysteretic loops and skeleton curves, load carrying capacity, ductility, equivalent stiffness, and energy dissipation. The experimental findings indicated that the mechanical performances of PC specimens with the modified connection form outperformed those of PC specimens with pure overlapping U-bar loop connections, closely resembling the properties of cast-in-place specimens; the failure mode of PC specimens was consistent with that of the CIP specimen; the generation, distribution and development of cracks in PC specimens were also similar to those in the CIP specimen. Furthermore, although the load-bearing capacity and peak displacement of PC specimens were lower than those of the CIP specimen due to the failure of the post-casted concrete strength to meet the requirements, the ductility, equivalent stiffness, and energy dissipation of PC specimens with the modified connection form closely matched that of the CIP specimen.

Green solvents such as water and ionic liquids (ILs) are pillars of the great mansion of green chemistry and green processing. Initially proposed as a new family of ILs, deep eutectic solvents (DESs) have received fast development in the past two decades. In this contribution, DESs are reviewed critically and the concept is extended to low-melting mixture solvents (LoMMSs), which cover all kinds of materials including ionic compounds, molecular compounds, and metals. Six classes of LoMMSs are proposed as the new classification system and examples are given. Finally, several thermodynamic issues concerning LoMMSs are discussed. Two new concepts, robustness of LoMMSs and high-entropy LoMMSs, are proposed.

Different crumb rubber (CR) particle sizes affect asphalt performance differently. To improve the performance of CR in SBS/CR composite modified asphalt (SBS/CRMA) and to study the enhancement of SBS/CRMA performance with varying ratios of composite CR particle sizes. This study employed three different configurations of SBS/CRMA composite ratios. Several tests and analyses were conducted, Brookfield viscosity measurements, conventional performance tests, storage stability, high- and low-temperature rheology, LAS, Fourier transform infrared spectroscopy (FTIR) and fluorescence microscopy (FM) analysis. The results indicated that, in the composite CR particle size ratio, a finer particle size of CR demonstrated more significant benefits in improving the high- and low-temperature performance of asphalt. However, with aging, the role of a coarse particle size of CR gradually became more prominent. Furthermore, compared to a single CR mesh SBS/CRMA, the 3 S + 5R-6:2:2 composite CR particle size ratio of SBS/CRMA exhibited superior high- and low-temperature deformation and fatigue resistance. Considering the microscopic analysis, it can be observed that the fine particle size of CR exhibited superior compatibility and anti-aging performance compared to the coarse particle size. In the composite CR particle size ratio, adjusting the proportion of coarse and fine CR particle size will improve the performance of SBS/CRMA significantly. Contrary to common belief, it was not necessarily true that a higher ratio of coarse CR particles leads to a better modification effect in SBS/CRMA. On the other hand, the composite CR particle size ratio proved significantly advantageous, thereby providing valuable guidance for enhancing the performance of SBS/CRMA.

This paper aims to address the problems of safety and durability in China’s ballastless track structures, particularly the lack of accurate analysis and methods for predicting the reliability of the new type of prefabricated track structure during the design phase. We propose a reliability prediction method for a new prefabricated track structure, the modular assembled track structure with built-in position retention. By adopting the fuzzy Failure Modes, Effects, and Criticality Analysis (fuzzy FMECA) method, a comprehensive assessment of fault severity, fault occurrence probability, and fault detection difficulty is conducted on the CRTS II slab track structure and the modular assembled track structure with built-in position retention. Consequently, a fault mode hazard assessment model for the new prefabricated track structure is constructed. Based on the assessment model and using a similar product method, a reliability prediction model for the new prefabricated track structure is established, and reliability prediction for the track structure is conducted. The research results indicate that the modular assembled track structure with built-in position retention has lower hazard levels and higher reliability compared to the CRTS II slab track structure. This study provides a scientific basis for the design optimization of new prefabricated track structures, helping to improve their safety and reliability, reduce operating and maintenance costs, and thereby promote the green and low-carbon development of the railway.

An orthogonal test of the component design of aerated concrete was carried out to solve the problems of low strength, easy cracking, and high water absorption of aerated concrete. The influences of cement content, water-binder ratio, foaming agent content, and polypropylene fiber content on the performances of aerated concrete including dry density, compressive strength, and thermal conductivity were investigated. Furthermore, the optimal composition of aerated concrete was determined. The test results showed that the dry density, compressive strength, thermal conductivity, and water absorption of aerated concrete were greatly influenced by the dosage of the foaming agent. Additionally, the water-binder ratio was found to significantly influence the apparent porosity of aerated concrete. Based on the experiment, the optimal combination of components for aerated concrete was 65 wt.% cement and 35% wt.% mineral admixture with a fly ash to slag ratio of 3:1. Meanwhile, the content of water-binder ratio, foaming agent, and polypropylene fibers were 0.4, 4 wt.%, and 0.4 wt.% of the total cementitious material, respectively. Among the mix proportions in this study, aerated concrete prepared by using the above mix proportion had the best performances. Subsequently, the influence of ceramsite content on the strength, dry density, and thermal conductivity of aerated concrete was analyzed. The results showed that the dry density and thermal conductivity of ceramsite aerated concrete increased with the increase of ceramsite content, but the change law of the water absorption was the opposite. Moreover, the compressive strength increased first and then decreased with the increase of ceramsite content, while the apparent porosity was the opposite. The optimal ceramsite content in ceramsite aerated concrete was 20 wt.%.

Abstract The reversible transformation of a nanohelix is one of the most exquisite and important phenomena in nature. However, nanomaterials usually fail to twist into helical crystals. Considering the irreversibility of the previously studied twisting forces, the reverse process (untwisting) is more difficult to achieve, let alone the retwisting of the untwisted crystalline nanohelices. Herein, we report a new reciprocal effect between molecular geometry and crystal structure which triggers a twisting-untwisting-retwisting cycle for tri-cobalt salicylate hydroxide hexahydrate. The twisting force stems from competition between the condensation reaction and stacking process, different from the previously reported twisting mechanisms. The resulting distinct nanohelices give rise to unusual structure elasticity, as reflected in the reversible change of crystal lattice parameters and the mutual transformation between the nanowires and nanohelices. This study proposes a fresh concept for designing reversible processes and brings a new perspective in crystallography.

China has emerged as a prominent global player in the field of railways, with numerous railway construction projects spanning across diverse locations. Railway bridges, as a crucial component of railway construction, warrant significant attention. Meta-analysis, a statistical method that systematically synthesizes research findings, has been utilized to summarize and compare the results of safety risk management studies pertaining to railway bridge construction in China. By integrating social network analysis and evidence-based assessment of the literature, this study explores the interrelationships among risk factors. Within a specific railway bridge construction project, various safety risk factors may originate from common sources, including environmental factors, material and equipment factors, technical factors, management factors, personnel factors, and bridge-specific factors. Notably, there exists coupling among these security risk factors, whereby the presence or occurrence of one factor can influence the probability or severity of consequences associated with other factors. The results reveal that safety risk factors in railway bridge construction accumulate and propagate, thereby impacting the efficacy of safety risk management. Moreover, these factors are significantly influenced by the complexities inherent in the geo-meteorological and social-technical systems. This finding provides valuable insights for innovations in security risk management practices and offers suggestions for future innovation pathways.

The solar temperature field and its temperature effects on the straddle monorail tourist transportation system (SMTTS) significantly affect its mechanical performance. To investigate the distribution law of the daylight temperature field of the unpaved steel beams, the daylight temperature fields of box-type and I-sectional steel beams in different regions were measured and simulated. With the simulation results, the vertical temperature gradient curves of the unpaved steel girders were determined. Afterward, with the historical meteorological data as variables, the parameters of the extreme value model of generalized extreme value (GEV) distribution were obtained, and the representative values of temperature differences in China, based on the recurrence period, were analyzed. By analyzing the temperature difference values in 34 cities in China, an empirical prediction formula for the representative temperature difference value with three parameters was proposed, and the temperature gradient partition was established. The results indicated that the vertical temperature gradients of closed-section steel box girders are about 2–3 °C greater than that of open-section I-beams. Moreover, there are apparent geographical differences in the temperature difference values of steel girders.

To investigate the fatigue and freeze-thaw properties of the geotextile isolation layer in CRTS III ballastless track, a new type of disc multi-point load distribution device was manufactured. The experimental study was carried out on the geotextile isolation layer subjected to the fatigue load and freeze-thaw cycles. The results showed that the mechanical properties of the geotextile isolation layers were affected by the harsh environment and cyclic load. With the increase of fatigue loading cycles, the dynamic stiffness of the geotextile isolation layers increased and tended to be stable, and the nonlinear performance of the geotextile isolation layer gradually weakened. The freeze-thaw cycle greatly influenced the dynamic stiffness of the geotextile isolation layer compared with the fatigue loadings, and the freeze-thaw cycle action could accelerate the elastic loss of the geotextile isolation layer. Under the fatigue load and freeze-thaw cycles, the tensile strength of the geotextile isolation layer decreased and caused damage to the geotextile isolation layers, which should be paid attention to in long-term service.

Wind flow over complex terrain is strongly influenced by the topographical features of the region, resulting in unpredictable local wind characteristics. This paper employs numerical simulation to study the wind flow at a towering peak extending out of a steep mountainside and the wind-induced effect on onsite landscape platforms. First, the wind flow from seven different directions is explored via 3D numerical simulations, and the wind load distribution on the platforms is highlighted. Second, a 2D numerical simulation is conducted to evaluate the wind speedup effect at the side peak, examining the influence of the side peak height and the mountainside steepness on the wind speedup factor. The numerical simulations presented in this research were validated by replicating a published numerical and experimental study. The results illustrate the amplifying and blocking effects of the surrounding topography, yielding unpredictable and nonuniform wind pressure distribution on the platforms. The presence of the side peak leads to a significant increase in the speedup factor, and the side peak height and the mountainside steepness have a moderate influence on the value of the speedup factor. Additionally, the speedup factor obtained from this study varies significantly, especially near the surface, from the recommendations of several wind load standards. Consequently, the impact of the local terrain and the wind speedup effect must be thoroughly assessed to ensure the structural integrity of structures installed at a similar topography.

In this article, a microscopic constitutive model is established that includes friction, plastic, and spring elements and has clear physical meaning. The friction unit reflects the mutual friction between crack surfaces, the plastic unit reflects the development of concrete plasticity, and the fracture of the spring unit reflects the formation and expansion of interior cracks in concrete. In addition, the integration of the random field theory into this model uncovers the physical underpinnings of the relationship between concrete’s nonlinearity and randomness. The multi-scale modeling of the concrete static damage constitutive model is then realized once the parameters of the random field are discovered using the macro test results. In order to apply the model’s applicability in finite element programs, a subroutine was ultimately constructed. The experimental data and the anticipated values from the numerical simulation are in good agreement, supporting the model’s realism.

This study investigated the bonding strength between various cement-based grouting materials (CBGM) and high-strength bolts, the flexural and compressive strength variation of different CBGM with curing age, and the failure characteristics of flexural, compressive and bonding strengths. Moreover, the morphological characteristics of bond-slip curves and their similarities and differences were discussed as well. Based on the mechanical property and safety analysis, a calculation model of the critical anchorage length of high-strength bolt in CBGM was proposed. The results indicate that the flexural and compressive strengths increase with the increase of curing age. The strength growth rate is faster at the initial stage of 3 days, which implies that the CBGM has a significant high-early strength characteristic. Depending on the diameter and type of high-strength bolts employed, the bonding strength of specimens can be divided into two failure forms, i.e., splitting and slipping pull-out. The specimens with threaded bolts easily take place a splitting failure form, but the specimens with plain round bolts tend to be destroyed with a slipping pull-out failure form. The ascending section of bond-slip curve is well fitted with the same stage of CEB-FIP 2010. However, a linear relationship can be employed to characterize the bonding strength between CBGM and high-strength bolt. The bond failure capacity is also linear correlation with the strength of CBGM.

Abstract Both proton exchange membrane fuel cells and uranium‐based nuclear techniques represent two green and advanced energies. However, both of them still face some intractable scientific and industrial problems. For the former, established proton‐conduction materials always suffer one or another defect such as low proton conductivity, high activation energy, bad durability, or just small‐scale product; while for the later, there still lacks available adsorbent to selectively recover of UO22+ from concentrated nitric acid (>1 M) during the spent fuel reprocessing due to the deactivation of the adsorption site or the decomposition of adsorbent under such rigorous conditions. It is found that the above two issues can be well solved by the construction of sulfonic‐pendent vinylene‐linked covalent organic frameworks (COFs), since these COFs contain abundant sulfonic units for both intrinsic proton conduction and UO22+ capture through strong coordination fixation and vinylene linkage that enhances the stability up to 12 M nitric acid (one of the best materials surviving in 12 M HNO3).

According to the assumption of small strains and total strain decomposition of cast iron, the expressions of the inelastic principal strain rate and relative energy dissipation rate of cast iron are derived, and the parameters of the tensile and compressive damage ratio of cast iron in the inelastic phase are proposed. Based on the least energy consumption principle, combined with the above expressions and parameters, the damage ratio strength criterion for cast iron under biaxial stress states is established. The damage ratio variable expression of the cast iron damage ratio strength criterion considering the effect of Lode angle is proposed. Furthermore, the values of the damage ratio variable are verified by the experimental stress–strain curves of cast iron under uniaxial stress states. By comparing the predicted values to 104 experimental data points of cast iron specimens, the failure envelopes of the proposed criterion can represent the failure behavior of cast iron under biaxial loading conditions. To facilitate the practical application in engineering, the further simplified expression of the four-parameter damage ratio strength criterion for cast iron under biaxial stress states is suggested and those empirical parameters are recommended. Compared with some main strength criteria, the simplified criterion is in better agreement with the experimental results under every stress state.

The ultra-high performance concrete (UHPC) was prepared by using the limestone coarse aggregate and ordinary Portland cement. The effects of sand ratio, water to cement ratio (W/C), cementitious materials, type and content of fiber, dosage of water reducer and defoamer on the mechanical and rheological properties of the UHPC were investigated. The correlations among influence factors and performances of UHPC were discussed. Moreover, the internal relationship between loading rate and elasticity modulus of UHPC was also studied. The results indicate that the compressive strength of UHPC first increases and then decreases with the increase of sand ratio, and a maximum compressive strength of UHPC was found at the sand ratio of 41%. The water reducer content of fresh UHPC firstly decreases and then increases with the increase of sand ratio. The compressive strength of UHPC firstly increases and then decreases with the increase of W/C, which also increases with the increase of cementitious materials. The elasticity modulus of UHPC decreases with increase of sand ratio and cementitious materials content, and the compressive strength and elasticity modulus of UHPC cured for 28 d increase with the increase of defoamer dosage. The slump flow of fresh UHPC first increases and then decreases with the increase of water reducer dosage. An optimum fiber content of UHPC can enhance the tensile strength and adhesive property, which can play the restraining deformation and toughening effect.

Heavy-haul railways have a high passing frequency of trains with a large axle weight, causing rapid accumulation of fatigue damage in reinforced concrete (RC) bridge structures, which significantly affects the safety of the bridges. To study the fatigue reliability of RC beams in heavy-haul railways, the fatigue performance function for RC beams in heavy-haul railways was established, and the fatigue reliability assessment method for bridge structures in heavy-haul railways based on the point estimate method (PEM) was developed. An 8 meter-span plate beam in an existing heavy-haul railway illustrates the method. The train axle weight and dynamic coefficient were considered random variables, and the first four moments of equivalent stress ranges were obtained. The traffic quantity of the heavy-haul railways was investigated, and the fatigue reliability was evaluated using the proposed method. In addition, the effects of annual freight volume and train axle weight on fatigue reliability were discussed. Results indicate that PEM can effectively and accurately evaluate the fatigue reliability of RC beams in heavy-haul railways. In the first 20 years of operation, the fatigue failure probability was less than the limit value specified in the standard. The increase in annual traffic volume and train axle weight will cause a significant increase in fatigue failure probability. The research results of this paper are expected to provide an important basis for the design and maintenance of reinforced concrete bridges for heavy-haul railways in the future.

The fundamental understanding of metal-organic frameworks (MOFs) is crucial since the relationship between the macroscopic properties of these materials and their molecular-level structures allows for the development of technological applications and improvements in current performance. The metal centers and attached functional groups dictate MOFs' dynamics, structure, and porosity. The development of the solid-state nuclear magnetic resonance (SSNMR) technique, capable of providing atomic-level information, enables the non-destructive characterization of the structure and dynamics of MOFs have become essential step in ameliorating our understanding and are often complementary to traditional methods. This review aims to briefly introduce the concepts of SSNMR and the methods employed when investigating the dynamics, structure, and mapping of functional groups of microporous materials, including MOFs. This review highlights the best experimental practices when working with these complex systems. The article scrutinizes the information on framework structures, active center, surface position, host-guest interaction, and intermediate interaction through different SSNMR spectrums. Despite all the recent technological advancements, the SSNMR still faces the challenges of large sample quantities, long experimental measurements and data analyses, and complex isotopic labeling, which are enlightened in this review.

The mix proportion, phase diagram components and mechanical properties of ultra-high performance mortar (UHPM) based on simplex-centroid design method were investigated. The effects of material components, sand/binder ratio (S/B) and water/binder ratio (W/B) on the strength and fluidity of UHPM were studied. Simultaneously, the relationship between water reducer dosage and slump flow of UHPM was investigated as well as the mix proportion design and performance prediction. The results showed that the simplex-centroid design method can be used to determine the relationship between influence factors and performance of UHPM. The differences of strength phase diagram for different mix proportion of UHPM were manifested in central position of minimum strength, strength contours and gradient, and position of maximum strength. There has a quadratic function relationship between water reducer dosage, slump flow and W/B. The slump flow of UHPM increases with the increase of water reducer dosage. The UHPM can be prepared based on simplex-centroid experimental design method.

To minimize the adverse effects of high temperatures on the service performance of track structures, research on the application of reflective coatings on track structures is urgently needed. Based on meteorological data and the characteristics of the multi-layer structure of the ballastless track, refined finite element models (FEMs) for the temperature field and temperature effect analysis of the CRTS III slab ballastless track structure on bridges were established. The temperature deformation characteristics and temperature stress distribution of the CRTS III slab ballastless track under natural environmental conditions were investigated. Similarly, the influence of a reflective coating on the structural temperature field and temperature effect was studied. The results showed that the temperature and vertical temperature gradient of the track slab were significantly reduced after the application of the reflective coating. Meanwhile, the thermal deformation and thermal stresses of the track slab and the self-compacting concrete (SCC) layer were minimized. Under high-temperature conditions in summer, the maximum temperature of the track slab decreased from 47.0 °C to 39.6 °C after the application of the reflective coating, and the maximum vertical temperature gradient of the track slab decreased from 61.5 °C/m to 39.1 °C/m after the application of the reflective coating. Under the maximum positive temperature gradient, the peak displacement of the upper arch in the middle of the slab and the peak displacement of the sinking in the slab corner decreased from 0.814 mm and 1.240 mm to 0.441 mm and 0.511 mm, respectively, and the maximum transverse tensile stresses of the track slab reduced from 2.7 MPa to 1.5 MPa as well. In addition, the reflective coating could also inhibit the failure of the interlayer interface effectively. The results of this study can provide a theoretical basis and reference for the application of reflective coatings on ballastless tracks on bridges.

This study investigated the tensile and bonding properties between cement-based grouting materials (CBGM) and high-strength bolts. The associated failure mechanism, load-slip curve, ultimate pull-out load and bond stress were also studied. The effects of anchorage length and square steel tube restraint on the bonding properties were explored on the basis of 24 specimens used in central pull-out testing, and a bond stress–slip constitutive relationship model between high-strength bolts and CBGM was proposed. The results indicate that with the increase in the anchorage length of high-strength bolts, the failure modes of specimens can be divided into three types: the fracture failure of high-strength bolt that occurred when the anchorage lengths ranged from 18 d to 31 d, the specimens that experienced splitting failure with the constraint of square steel tube when the anchorage length was less than 15 d and the high-strength bolt that experienced pull-out failure without the constraint of square steel tubes. When the high-strength bolt experiences tensile failure, the ultimate pull-out load remains constant and the bond stress decreases as the anchorage length of high-strength bolts increases. Due to the lateral constrained effect of the square steel tube, the CBGM embodies a three-dimensional stress state, which can delay the generation and development of internal cracks and enhance the bond strength. A calculation formula was proposed to determine the bond strength between high-strength bolt and CBGM, and a constitutive model of the bond stress–slip constitutive relationship was modeled.

Simply supported prestressed concrete (PC) box girders have been widely adopted in high-speed railway bridges. In complex climatic environments, the corrosion of the prestressing strands always occurs and deteriorates the flexural behavior of PC box girders. In the present study, six T-shaped scaled beams were designed and fabricated according to the specifications for a high-speed railway PC box girder. The corrosion process of the prestressing strand in scaled beams was experimentally simulated by using the constant current accelerated corrosion method. The flexural behavior of corroded high-speed railway simply supported PC box girders was then investigated through four-point bending tests and theoretical investigation. The experimental results showed that strand corrosion significantly decreased the flexural behavior of the test beams. When the mass loss was 12.30%, the cracking load, ultimate load, and ductility decreased by 27.8%, 29.9%, and 11.5%, respectively. The effect of strand corrosion on flexural stiffness displayed a difference before and after concrete cracking. The failure mode changed when strand mass loss was above a critical value (7%). The flexural bearing capacity degradation law of corroded PC beams could be divided into two distinct stages. A strand mass loss of less than 7% could lead to a linear degradation law with a relatively slight reduction. As mass loss increased, it exhibited an exponential and sharp declining trend. An analytical model including the effects of strand cross-section reduction, strand property deterioration, and concrete cracking was also proposed to predict the flexural behavior of corroded PC beams. By comparison with the experimental data, it was found that the model could predict the cracking moment, flexural bearing capacity, and failure mode well.

The concrete track slab and the base slab of the high-speed railway CRTS II track structure are prone to transverse cracks in the initial service period, which are subjected to environmental action and train load. In order to investigate the influence of transverse cracks on chloride ingress of concrete track slab and base slab in a coastal environment, drying-wetting cycle chloride erosion tests were carried out on reinforced concrete track slab and base slab specimens with cracks ranging from 0 mm to 0.6 mm, subjected to continuous bending moment. The chloride ion concentration of concrete along the depth direction was collected during the test process. The experimental results show that the chloride ion concentration of concrete at the crack section is much higher than that at the intact section, and it increases with the increase of crack width in the range of 0.2 mm to 0.6 mm. A chloride diffusion coefficient model of cracked concrete is proposed for slab track based on the experimental results, which can comprehensively consider the effects of surface chloride ion concentration, chloride binding effect, time-varying effect, temperature, relative humidity, and transverse crack width.

In the era of energy conservation and environmental protection, as well as the industrialization of buildings, precast concrete (PC) structures have been developed and increasingly applied in construction industries due to their advantages of outstanding workability and ecofriendliness. In order to verify the reliability of overlapping U-bar loop connections and a modified form of these connections, and study the seismic performance of PC wall–beam–slab joints with these connection methods, three full-scale wall–beam–slab joints were designed and tested under low reversed cyclic loading, including one cast-in-place (CIP) specimen and two PC specimens. Based on the test results, the seismic performance of the PC joints was studied by comparing their damage process, hysteretic loops and skeleton curves, load-carrying capacity, ductility, equivalent stiffness, and energy dissipation with those of the CIP joint. After analyzing the experimental results, the following conclusions can be drawn: the overlapping U-bar loop connection and its modified form are effective and reasonable; the specimen with the modified connection form showed slightly better mechanical properties; the failure mode of the PC joints was consistent with that of the CIP joint; and the generation, distribution, and development of cracks in the PC specimens were similar to those in the CIP specimen. In addition, the stiffness of the PC joints was similar to that of the CIP joint, and the load-carrying capacity, ductility, and energy dissipation of the PC joints were better than those of the CIP joint. Moreover, the research in this paper can also provide some guidance for assembling wall–beam–slab joints in PC shear wall structures.

Abstract Objective MicroRNAs (MiRNAs) is thought to play a critical role in the initiation and progress of ovarian cancer (OC). Although miRNAs has been widely recognized in ovarian cancer, the role of hsa-miR-30a-5p (miR-30a) in OC has not been fully elucidated. Methods Three mRNA datasets of normal ovarian tissue and OC, GSE18520,GSE14407 and GSE36668, were downloaded from Gene Expression Omnibus (GEO) to find the differentially expressed gene (DEG). Then the target genes of hsa-miR-30a-5p were predicted by miRWALK3.0 and TargetScan. Then, the gene overlap between DEG and the predicted target genes of miR-30a in OC was analyzed by Gene Ontology (GO) enrichment analysis. Protein-protein interaction (PPI) network was conducted by STRING and Cytoscape, and the effect of HUB gene on the outcome of OC was analyzed. Results A common pattern of up-regulation of miR-30a in OC was found. A total of 225 DEG, were identified, both OC-related and miR-30a-related. Many DEG are enriched in the interactions of intracellular matrix tissue, ion binding and biological process regulation. Among the 10 major Hub genes analyzed by PPI, five Hub genes were significantly related to the overall poor survival of OC patients, in which the low expression of ESR1,MAPK10, Tp53 and the high expression of YKT,NSF were related to poor prognosis of OC. Conclusion Our results indicate that miR-30a is of significance for the biological progress of OC.

The development of fatigue damage in reinforced concrete (RC) beams is affected by various factors such as repetitive loads and material properties, and there exists a complex nonlinear mapping relationship between their fatigue performance and each factor. To this end, a fatigue performance prediction model for RC beams was proposed based on the deep belief network (DBN) optimized by particle swarm optimization (PSO). The original database of fatigue loading tests was established by conducting fatigue loading tests on RC beams. The mid-span deflection, reinforcement strain, and concrete strain during fatigue loading of RC beams were predicted and evaluated. The fatigue performance prediction results of the RC beam based on the PSO-DBN model were compared with those of the single DBN model and the BP model. The models were evaluated using the R2 coefficient, mean absolute percentage error, mean absolute error, and root mean square error. The results showed that the fatigue performance prediction model of RC beams based on PSO-DBN is more accurate and efficient.

Abstract This paper proposes a new kind of U-type reinforcement ferrule (URF) connection for the assembly of precast concrete (PC) components, which has a good fault-tolerance ability and low cost of construction with a simple post-grouting process. The modified connection features welded U ferrules, which can increase the bonding mechanism and internal stress transmission in the spliced region. A series of quasi-static cyclic tests were performed on URF-connected PC columns with different welding patterns. Comprehensive comparisons were performed on the load-bearing capacity, the plastic energy dissipation ability, the internal force transmission, the column ductility and the stiffness. The results indicated that PC columns with welded URF connections can ensure better lateral resistance than the cast-in-place concrete column. The weld type and length influence the connection performance, and the 15d-welded and full-length welded URF connections presented better connection performance and were suggested for the assembly of PC columns. Then, finite element analyses were performed to further reveal the working and failure mechanisms and the affecting mechanism of some working parameters. Parametric simulations indicated that the increase in the axial load ratio and the concrete strength in the postcast region had a slight but limited effect on improving the lateral resistance of the column connection.

The whole-genome sequencing (WGS) of human adenoviruses (HAdVs) plays an important role in identifying, typing, and mutation analysis of HAdVs. Nowadays, three generations of sequencing have been developed. The accuracy of first-generation sequencing is up to 99.99%, whereas this technology relies on PCR and is time consuming; the next-generation sequencing (NGS) is expensive and not cost effective for determining a few special samples; and the third-generation sequencing technology has a higher error rate. In this study, first, we developed an efficient HAdV genomic DNA extraction method. Using the complete genomic DNA instead of the PCR amplicons as the direct sequencing template and a set of walking primers, we developed the HAdV WGS method based on first-generation sequencing. The HAdV whole genomes were effectively sequenced by a set of one-way sequencing primers designed, which reduced the sequencing time and cost. More importantly, high sequence accuracy is guaranteed. Four HAdV strains (GZ01, GZ02, HK35, and HK91) were isolated from children with acute respiratory diseases (ARDs), and the complete genomes were sequenced using this method. The accurate sequences of the whole inverted terminal repeats (ITRs) at both ends of the HAdV genomes were also acquired. The genome sequence of human adenovirus type 14 (HAdV-B14) strain GZ01 acquired by this method is identical to the sequence released in GenBank, which indicates that this novel sequencing method has high accuracy. The comparative genomic analysis identified that strain GZ02 isolated in September 2010 had the identical genomic sequence with the HAdV-B14 strain GZ01 (October 2010). Therefore, strain GZ02 is the first HAdV-B14 isolate emergent in China (September 2010; GenBank acc no. MW692349). The WGS of HAdV-C2 strain HK91 and HAdV-E4 strain HK35 isolated from children with acute respiratory disease in Hong Kong were also determined by this sequencing method. In conclusion, this WGS method is fast, accurate, and universal for common human adenovirus species B, C, and E. The sequencing strategy may also be applied to the WGS of the other DNA viruses.

The effects of the type and content of fibers, water to cement ratio (W/C), and content of cementitious materials on the shrinkage and creep of ultra-high performance concrete (UHPC) were investigated. The relationships between curing age, shrinkage, and unit creep of the UHPC were also discussed. The results showed that the shrinkage of the UHPC decreased with the increase in W/C, where there existed a quadratic function between shrinkage and W/C. However, the unit creep of the UHPC increased with W/C. The shrinkage and unit creep of the UHPC increased with the increase in the content of the cementitious materials. The type and content of fibers had different effects on the shrinkage and unit creep of the UHPC, that is, the shrinkage of the UHPC first increased and then decreased with the increase in the content of steel fibers, where there existed a quadratic function between them. There was a linear function between the shrinkage of the UHPC and the content of carbon fibers, but the shrinkage of the UHCP first increased and then decreased with the increase in PVA content. The shrinkage and unit creep of the UHPC at the initial curing age were significant, which tended to be constant with the increase in curing age. Although the steel fibers had a significant inhibiting effect on the unit creep of the UHPC, the carbon fibers and PVA had positive and negative effects on the unit creep of the UHPC. The effects of the type and content of fibers on the shrinkage and unit creep of the UHPC were caused by the slenderness ratio, shape, surface roughness, and elasticity modulus of the fibers. The shrinkage and creep of the UHPC were caused by the chemical autogenous shrinkage and free water evaporation of the UHPC.

Due to the demand for increasing trainload and enhancing some existing heavy-haul railways, the low reserve value of bearing capacity is a problem for the 32 m-span simply supported beam. The fatigue behavior of prestressed concrete beams in a heavy-haul railway loaded by 33 t and larger axle weight of trains was experimentally investigated. The experimental results of the fatigue behaviors, including fatigue deformation, crack propagation behavior, and strains of classical materials were obtained and analyzed. A fatigue behavior assessment model was established to investigate the residual stiffness and yield point degradation of the beams loaded by the trainload. The effects of train fatigue cycles and prestress loss on the residual stiffness and yield point degradation models of the beams were analyzed. The results indicated that the crack development process had three stages during the fatigue process: the derivative stage, gradual development stage, and fatigue failure stage. Trainload was the main external factor influencing the fatigue behavior of prestressed concrete beams. The increase in trainload accelerated the degradation rate of the residual stiffness of the beams and yield point, reducing the fatigue life. The prestressing strand was primarily used to delay the concrete cracking in the tension zone. When the beam was not cracked, the prestressed concrete beam showed good fatigue performance, and the degree of prestressing did not affect the fatigue life of the beams. When the maximum fatigue load exceeded the cracking load of the beam, prestress loss in beams became a critical issue that accelerated the degradation rate of fatigue strength and reduced fatigue life. The higher the fatigue damage degree, the more pronounced the effect of prestress loss on the fatigue strength of the beams. The fatigue failure of prestressed concrete beams occurred in the bottom tensile steel bar. Therefore, when the trainload of a heavy-haul railway is greater than the cracking load of the beam, it is recommended to strengthen the beam by prestressing and strictly control the trainload to avoid yield failure.

The secondary development of ABAQUS was carried out to calculate the shrinkage and creep of concrete, and a finite element model of the China Railway Track System (CRTS) II slab ballastless track was established. Then, the interlaminar stress of CRTS II slab ballastless track at different ages of the track slab during laying (AOTSL) caused by concrete shrinkage and creep was studied. The obtained results showed that the stress redistribution occurred in the sliding layer, which resulted in the generation of a gap. Although the gap length was slightly reduced due to the shear cogging, the sliding layer at the slab edge is more prone to produce gaps. Under the effect of shrinkage and creep of the ballastless track, large additional shear stress, up to 0.676 MPa, was induced at the interface between CA mortar and the track slab. Meanwhile, the appearance of additional vertical and lateral forces of the shear cogging was caused by the shrinkage and creep of the ballastless track. Additionally, by further analysis, the recommended AOTSL ranges from 120 days to 180 days.

The trivalent seasonal influenza vaccine was the only approved and available vaccine during the 2016–2018 influenza seasons. It did not include the B/Yamagata strain. In this study, we report an acute respiratory disease outbreak associated with influenza B/Yamagata infections in Guangzhou, Southern China (January through March, 2018). Among the 9914 patients, 2241 (22.6%) were positive for the influenza B virus, with only 312 (3.1%) positive for the influenza A virus. The influenza B/Yamagata lineage dominated during this period in Southern China. The highest incidence of influenza A virus infection occurred in the children aged 5–14 years. In contrast, populations across all age groups were susceptible to the influenza B virus. Phylogenetic, mutations, and 3D structure analyses of hemagglutinin (HA) genes were performed to assess the vaccine-virus relatedness. The recommended A/H1N1 vaccine strain (A/Michigan/45/2015) during both 2017–2018 and 2018–2019 was antigen-specific for these circulating isolates (clade 6B.1) in Spring 2018. An outbreak of influenza B/Yamagata (clade 3) infections in 2018 occurred during the absence of the corresponding vaccine during 2016–2018. The recommended influenza B/Yamagata vaccine strain (B/Phuket/3073/2013) for the following season (2018–2019) was antigen-specific. Although there were only a few influenza B/Victoria infections in Spring 2018, five amino acid mutations were identified in the HA antigenic sites of the 19 B/Victoria isolates (clade 1A), when compared with the 2016–2018 B/Victoria vaccine strain. The number was larger than expected and suggested that the influenza B HA gene may be more variable than previously thought. One of the mutations (K180N) was noted to likely alter the epitope and to potentially affect the viral antigenicity. Seven mutations were also identified in the HA antigenic sites of 2018–2020 B/Victoria vaccine strain, of which some or all may reduce immunogenicity and the protective efficacy of the vaccine, perhaps leading to more outbreaks in subsequent seasons. The combined epidemiological, phylogenetic, mutations, and 3D structural analyses of the HA genes of influenza strains reported here contribute to the understanding and evaluation of how HA mutations affect vaccine efficacy, as well as to providing important data for screening and selecting more specific, appropriate, and effective influenza vaccine candidate strains.

Based on principles of chemical thermodynamics, the relationship between temperature and the Gibbs free energy of erosion products generated during the sulfate attack on cement concrete was deduced. The orientation of chemical reactions of sulfate attack on cement concrete was theoretically determined as well as the critical sulfate ion concentration and the formation conditions of erosion products. The phase composition, microstructure, crystal form, and morphology of erosion products before and after sulfate attack were investigated by environmental scanning electron microscope and energy spectrum analysis (ESEM-EDS) and X-ray diffraction (XRD). The results show that the effects of sulfate ion concentration and temperature on cement concrete sulfate attack are significant, and different influencing factors correlate with each other. The crystal transition temperature between the anhydrite and dihydrate gypsum is 42°C, and the corresponding concentration of sulfate ion is about 2.3 × 10−3 mol/L. Simultaneously, the crystal transition temperature between the thenardite and mirabilite is 32.4°C. Moreover, the theoretical upper limit temperature and sulfate ion lower limit concentration of thaumasite are 44°C and 0.0023 mol/L, respectively. The ESEM-EDS and XRD results imply that the chemical thermodynamics can be used to reveal the erosion mechanism of sulfate attack on cement concrete. The major erosion products of sulfate attack on cement concrete are rod-like ettringite with a larger slenderness ratio, plate-like gypsum, granular sulfate salt, incompletely corroded calcium hydroxide, and residual skeleton of calcium silicate hydrate. The sulfate attack has double effects on mechanical properties of specimens, which can affect the microstructure, phase composition, type, and morphology of erosion products.

The coupled action of concrete carbonation and repeated loading strongly influences the safety of reinforced concrete (RC) structures and substantially reduces service life. A novel corrosion-fatigue life prediction model for RC structures under coupled carbonation and repeated loading was developed. The effect of fatigue damage on concrete carbonation and carbonation-induced corrosion rate was considered, and the acceleration of fatigue damage accumulation due to reinforcement corrosion was considered in this approach. The proposed corrosion-fatigue life prediction model was illustrated by a 6 m-span RC slab in a simply supported slab bridge for the highway, and the effects of traffic frequency, overloading, carbonation environment grade, and environmental temperature and relative humidity on corrosion-fatigue life were discussed. The results indicate that the proposed model can predict the corrosion-fatigue life of RC structures simply and conveniently. Traffic frequency, overloading, carbonation environment grade, and environmental temperature and relative humidity can decrease the corrosion-fatigue life of the RC slab by up to 66.86%, 58.90%, 77.45%, and 44.95%, respectively. The research is expected to provide a framework for the corrosion-fatigue life prediction of RC structures under coupled carbonation and repeated loading.

The exposed facets of oxide nanocrystals are key to their properties. Here, the authors use 17O solid-state NMR spectroscopy to discriminate between oxygen species on different facets of anatase titania nanocrystals, providing compelling evidence for the value of NMR spectroscopy in characterizing faceted oxides.

Human adenovirus type 14 (HAdV-B14p) was originally identified as an acute respiratory disease (ARD) pathogen in The Netherlands in 1955. For approximately fifty years, few sporadic infections were observed. In 2005, HAdV-B14p1, a genomic variant, re-emerged and was associated with several large ARD outbreaks across the U.S. and, subsequently, in Canada, the U.K., Ireland, and China. This strain was associated with an unusually higher fatality rate than previously reported for both this prototype and other HAdV types in general. In China, HAdV-B14 was first observed in 2010, when two unrelated HAdV-B14-associated ARD cases were reported in Southern China (GZ01) and Northern China (BJ430), followed by three subsequent outbreaks. While comparative genomic analysis, including indel analysis, shows that the three China isolates, with whole genome data available, are similar to the de Wit prototype, all are divergent from the U.S. strain (303600; 2007). Although the genomes of strains GZ01 and BJ430 are nearly identical, as per their genome type characterization and percent identities, they are subtly divergent in their genome mutation patterns. These genomes indicate possibly two lineages of HAdV-B14 and independent introductions into China from abroad, or subsequent divergence from one; CHN2012 likely represents a separate sub-lineage. Observations of these simultaneously reported emergent strains in China add to the understanding of the circulation, epidemiology, and evolution of these HAdV pathogens, as well as provide a foundation for developing effective vaccines and public health strategies, including nationwide surveillance in anticipation of larger outbreaks with potentially higher fatality rates associated with HAdV-B14p1.Emerging Microbes & Infections (2017) 6, e92; doi:10.1038/emi.2017.78; published online 1 November 2017

This paper proposed a fast modeling technique (FMT) for the vertical dynamic analysis of the coupled train-track-bridge (TTB) systems, which combines the train subsystems and track-bridge subsystems by the client-server technique to complete the entire TTB analysis on simplex OpenSees simulation platform. Thus, the FMT could dramatically reduce the time consumed of programming and modeling and significantly reduce the amount of data transmission between subsystems for TTB interaction. Moreover, FMT could take full advantage of OpenSees in nonlinear and seismic analysis. So FMT is a practical and convenient approach to analyze the TTB coupling vibration, and especially it is suitable for the junior researchers of TTB interaction. FMT could improve the modeling efficiency to save time.