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IntroductionThe demand for sustainable food packaging materials has led to the exploration of bioactive composite films. This study aimed to prepare and evaluate a composite film made from sweet potato peel polyphenols extract and sweet potato starch (SPS) for its potential use in food packaging.MethodsThe composite film was prepared by uniformly dispersing 0.4% sweet potato peel polyphenols in the SPS matrix. Physicochemical properties and functional characteristics were assessed, including mechanical properties, UV barrier, water and gas barrier properties, antioxidant activity, and antimicrobial abilities against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. The film's efficacy in food packaging was tested using fresh cherry tomatoes, stored at 4°C, to determine its impact on shelf life.ResultsThe starch-based sweet potato peel polyphenols film demonstrated enhanced mechanical properties and excellent UV barrier properties. It showed improved water and gas barrier properties and strong antioxidant activity, with clearance rates above 90% for DPPH and ABTS radicals. The film also exhibited effective antimicrobial abilities against the tested bacteria. Food packaging experiments indicated that the film could extend the shelf life of fresh cherry tomatoes to 7 days when stored at 4°C.DiscussionThe results suggest that the developed composite film has significant potential as an eco-friendly food packaging material. Its multifunctional properties, including UV protection, barrier enhancement, antioxidant activity, and antimicrobial capabilities, make it a promising candidate for extending the shelf life of perishable foods. The film's performance in slowing spoilage and extending the shelf life of cherry tomatoes highlights its practical application prospects in the food industry.

The quick emergence of the COVID-19 pandemic has shaken the foundations of the world's economy. Notably, women entrepreneurs were significantly in danger of income and sales due to the COVID-19 pandemic, which has had significant economic and social impacts. This study examines the effects of the COVID-19 pandemic Standard Operating Procedures (SOPs) on women entrepreneurs’ sales performance, engaged in home-based activities. The chi-square test of association and linear probability model with interactions were applied to analyze the data collected using a questionnaire from 672 women entrepreneurs. Generally, cloth and cosmetics entrepreneurs reported an increase in sales. However, following the COVID-19 pandemic SOPs negatively affected women's entrepreneurial sales performance. Variations in findings were observed by testing the interaction and heterogeneous effects. The household contextual analysis is crucial for understanding the dynamics of women home-based entrepreneurs in the family and cultural context as they reported an increase in sales volume. The study's findings point to the adaptability, resilience, and multiplier effects of women entrepreneurs who faced economic, sociocultural, and institutional restraints encountered during the uncertain period of the COVID-19 pandemic.

IntroductionUnderstanding the microbial diversity and potential functional dynamics within the rhizocompartments of Dendrobium huoshanense is crucial for unraveling the plant–microbe interactions that influence its medicinal properties.MethodsThis study is the first to characterize the microbiome associated with the rhizocompartments of D. huoshanense, including its cultivation medium, rhizosphere, rhizoplane, and root endosphere, using high-throughput sequencing and subsequent bioinformatic analysis.ResultsBacterial phylogenetic diversity was significantly higher in the endosphere than in the rhizosphere, while fungal α-diversity significantly decreased from the cultivation medium to the endosphere. Both bacterial and fungal niche widths decreased from the cultivation medium to the endosphere. β-Diversity analysis revealed distinct spatial patterns in both bacterial and fungal communities across the rhizocompartments, with the most pronounced differences between the cultivation medium and the endosphere. Taxonomically, Proteobacteria and Ascomycota were predominant in the endosphere for bacterial and fungal communities, respectively. Functional predictions showed significant enrichment of pathways related to xenobiotics biodegradation, lipid metabolism, and nitrogen fixation in the endosphere, while functions associated with plant pathogens and saprotrophs were significantly reduced.DiscussionThe results indicate a shift from generalist to specialist microbes from the cultivation medium to the endosphere, suggesting that D. huoshanense exerts strong selective pressure for endophytic fungi. Interestingly, a high proportion of fungi with unknown functions were found in the endosphere, highlighting an area for further research regarding the medicinal efficacy of D. huoshanense. Overall, this study provides foundational data for understanding the adaptive evolution of these microbial communities in response to specific microhabitats.

Dendrobium officinale leaf contains various physiologically active substances. However, no previous studies have reported on the use of D. officinale leaf extract in fermented sweet potato beverages. In this study, Dendrobium officinale leaf and sweet potato were used as raw materials to produce a compound fermented beverage flavored with D. officinale leaf. A sweet potato vinegar folk production method was used, and beneficial microorganisms, including Rhizopus, yeast, and lactic acid bacteria, were inoculated into the fermentation. The quantity of D. officinale leaves, concentration of the sweet potato koji, fermentation time, and temperature during the fermentation process were optimized using orthogonal experiments and analysis of variance. The results indicated that a D. officinale leaf extract of 30%, sweet potato koji concentration of 30%, fermentation time of 5 days, and temperature of 30°C were optimal for beverage formation. Under these conditions, the fermentation broth had a mild flavor of D. officinale leaf and a moderately sweet and sour taste, the biofilm was a transparent gelatinous film, and the utilization efficiency of carbohydrates was high. Excellent flavor, color, and stability of the sweet potato beverage were obtained, with a total sugar content of 15.88 mg/mL and a final pH of 2.65.

Abstract Background For CD19-positive relapsed/refractory B-cell acute lymphoblastic leukemia (r/r B-ALL) after treatment with murine CD19 (mCD19) CAR-T, the reinfusion of mCD19 CAR-T cells may be ineffective due to anti-mouse single-chain variable fragment (scFv) antibody caused by mCD19 CAR. To overcome this immunogenicity, we applied humanized CD19 (hCD19) CAR-T cells to treat r/r B-ALL patients with prior mCD19 CAR-T therapy. Methods Nineteen pediatric and adult patients were included, 16 relapsed after and 3 were primarily resistant to mCD19 CAR-T. All patients presented with more than 5% blasts in bone marrow and/or extramedullary disease, and still showed CD19 antigen expression. Humanized CD19-CARs were lentiviral vectors carrying a second generation CAR with 4–1-BB co-stimulatory and CD3ζ signaling domains. Patient-derived cells were collected for producing CAR-T cells, the median dose of infused hCD19 CAR-T cells was 2.4 × 105/kg (range, 1.0–18.0 × 105/kg). Results hCD19 CAR-T resulted in a complete remission (CR) rate of 68% (13/19). Among 13 remission patients, 11 underwent allogeneic hematopoietic cell transplantation (allo-HCT) (3 were second HCT) and 10 remained in CR; the event-free survival rates at 12–18 months were 91% in 11 patients received following allo-HCT and 69% in all CR patients. Six cases had no response to hCD19 CAR-T, 3 died of disease progression; another 3 received salvage second transplantation, of them, 2 relapsed again (one died). Cytokine release syndrome (CRS) occurred in 95% (18/19) of patients, most CRS events were grade 1 and grade 2 (n = 17), there was only one grade 4 CRS. Two cases experienced grade 1 neurotoxicity. Conclusions Humanized CD19 CAR-T cell therapy could be a treatment option for CD19-positive B-ALL patients who relapsed after or resisted prior murine CD19 CAR-T, hCD19 CAR-T followed by allo-HCT provided a longer remission in CR patients. Nevertheless, the prognosis of non-responders to hCD19 CAR-T remained dismal. Trial registration Chinese Clinical Trial Registry/WHO International Clinical Trial Registry ( ChiCTR1900024456 , URL: www.chictr.org.cn ); registered on July 12, 2019.

We explore the dynamics and determinants of volatility connectedness between cryptocurrencies and energy. We employed a block dynamic equicorrelation model and a group volatility connectedness measurement to measure the cross-equicorrelation and volatility connectedness between cryptocurrencies and energy. We also adopted dynamic model averaging to identify the time-varying drivers. The results suggest that changes in cross-equicorrelation between the two groups were affected by influential global events and increased after the COVID-19 pandemic. Volatilities were transmitted in both directions between cryptocurrencies and energy, but the transmission from energy to cryptocurrencies is by far the strongest. The driver identification implies that the factors related to cryptocurrencies and global financial markets had important roles in explaining the volatility connectedness from cryptocurrencies to energy in some periods after the COVID-19 pandemic, but the effects were marginal. In contrast, factors such as electricity consumption, cryptocurrency turnovers, and VIX were important in affecting the volatility connectedness from energy to cryptocurrencies, and the effects depended on factors and changed over time.

Abstract Background To date, almost all studies regarding chimeric antigen receptor (CAR)‐T cell therapy for B‐cell acute lymphoblastic leukemia (B‐ALL) were performed in refractory/relapsed (r/r) or minimal residual disease‐positive patients. CAR‐T therapy in remission patients has not been reported. Aim To observe the treatment outcome of CAR‐T cells for remission B‐ALL patients with poor prognosis. Methods and Results CAR‐T treatment was applied to two B‐ALL patients in remission status who had poor prognostic factors and refused transplantation, and one case was unable to accept standard chemotherapy owing to multiple complications. The procedure of CAR‐T therapy in these two remission patients was the same as that in r/r B‐ALL patients. Lentiviral vectors encoding second generation CARs composed of CD3ζ and 4‐1BB were used to produce CAR‐T cells. Lymphodepleting agents fludarabine and cyclophosphamide were administered prior to cell infusion. Grade I cytokine release syndrome occurred after each T‐cell infusion and there was no neurotoxicity. CAR‐T treatment followed by non‐intensive maintenance chemotherapy and targeted drugs allowed both patients to obtain a long‐term event‐free survival of more than three and a half years without transplantation. Conclusions CAR‐T therapy could be used in high‐risk B‐ALL patients as a consolidation to avoid transplantation, the combination of CAR‐T and following maintenance therapy may be better than CAR‐T alone for durable remission.

Human Rhinoviruses (RVs) are dominant pathogens causing a wide range of respiratory tract diseases, posing a huge threat to public health worldwide. Viruses belonging to the RV-C species are more likely to cause severe illnesses and are strongly associated with asthma onset or exacerbations than RV-A or RV-B. Rapid and sensitive detection of neutralizing antibodies (NAbs) against RV-C can promote the development of vaccines and antiviral drugs and help in the diagnosis of viral infection. In this study, a rapid neutralization testing system for RV-C15, based on an enzyme-linked immunospot assay (Nt-ELISPOT) was developed. A monoclonal antibody (MAb), named 9F9, with high binding efficacy for RV-C15 conjugated to horseradish peroxidase (HRP), was used to detect RV-C15-infected cells at a concentration of 2 μg/ml. The optimal infectious dose of RV-C15 was set at 1 × 104 TCID50/well and the cells were fixed with 0.5% formaldehyde diluted in PBS after incubation for 20 h. Compared with the traditional cytopathic effect (CPE)-based neutralization assay (Nt-CPE), Nt-ELISPOT significantly shortened the detection period and showed good consistency with the detection of neutralizing titers of both sera and NAbs. Using Nt-ELISPOT, three anti-RV-C15 NAbs were obtained with IC50 values of 0.16, 0.27, and 11.8 μg/ml, respectively. Moreover, 64 human serum samples collected from a wide range of age groups were tested for NAb against RV-C15 by Nt-ELISPOT. The total seroprevalence was 48.4% (31/64) and the positive rate was lowest in the group under 6 years old. Thus, the Nt-ELISPOT established in this study can be used as a high-throughput and rapid neutralization assay for the screening of NAbs and for seroepidemiological investigation against RV-C15.

The COVID-19 infections have profoundly and negatively impacted the whole world. Hence, we have modeled the dynamic spread of global COVID-19 infections with the connectedness approach based on the TVP-VAR model, using the data of confirmed COVID-19 cases during the period of March 23rd, 2020 to September 10th, 2021 in 18 countries. The results imply that, (i) the United States, the United Kingdom and Indonesia are global epidemic centers, among which the United States has the highest degree of the contagion of the COVID-19 infections, which is stable. South Korea, France and Italy are the main receiver of the contagion of the COVID-19 infections, and South Korea has been the most severely affected by the overseas epidemic; (ii) there is a negative correlation between the timeliness, effectiveness and mandatory nature of government policies and the risk of the associated countries COVID-19 epidemic affecting, as well as the magnitude of the net contagion of domestic COVID-19; (iii) the severity of domestic COVID-19 epidemics in the United States and Canada, Canada and Mexico, Indonesia and Canada is almost equivalent, especially for the United States, Canada and Mexico, whose domestic epidemics are with the same tendency; (iv) the COVID-19 epidemic has spread though not only the central divergence manner and chain mode of transmission, but also the way of feedback loop. Thus, more efforts should be made by the governments to enhance the pertinence and compulsion of their epidemic prevention policies and establish a systematic and efficient risk assessment mechanism for public health emergencies.

With the improvement of inclusive financial system, China’s economy has made significant development and growth. It worth in-depth investigation on environmental impact of financial inclusion, since growing GDP usually accompanied by more intensive carbon emission. This paper aims to reveal whether financial inclusion contributes to the carbon reduction in China using county-level dataset. A fixed-effect panel regression approach is adopted to examine the impact of financial inclusion on county-level regional carbon emissions. The estimation results imply that financial inclusion plays an important role in reducing carbon emissions. The mediation effect analysis reveals two channels through which financial inclusion imposes negative impact on the level of regional carbon emissions. One is to elevate the carbon sequestration capacity by increasing vegetation coverage, and the other is to improve the industrial structure through enhanced financial support. In addition to being a bridge between economic opportunity and output, financial inclusion can also act as an effective measure for addressing climate change.

How to utilize financial instrument to deal with environmental issues has been a focal topic. Taking the introduction of green credit program as a “quasi-natural experiment,” the propensity score matching and difference-in-difference approach (PSM-DID) are used to investigate the impact of the green credit policy implemented by Chinese government on firm-level industrial pollutant emissions. The estimation results indicate that the green credit policy significantly reduces corporate sulfur dioxide emissions. Heterogeneity analysis shows this impact is more pronounced for large-scale enterprises and enterprises located in the eastern region. The estimated mediation models reveal that after the implementation of the green credit policy, reduction in sulfur dioxide emissions can be attribute to the increased environmental investment and improved energy consumption intensity. Moreover, the green credit policy is also significantly effective in mitigating the discharge of other common industrial pollutants. Our findings highlight the importance of green credit policies in achieving greener industrial production and more sustainable economic development.

BackgroundReduced intensity conditioning (RIC) before allogeneic hematopoietic stem cell transplantation (allo-HSCT) has been reported to have the same overall survival (OS) as myeloablative conditioning (MAC) for patients with acute myeloid leukemia (AML) in complete remission (CR) and myelodysplastic syndrome (MDS). However, results from different studies are conflicting. Therefore, we conducted a systematic review and meta-analysis guided by PRISMA 2009 to confirm the efficacy and safety of RIC vs. MAC for AML in CR and MDS.MethodsWe search PubMed, Web of Science, Embase, Cochrane central, clinical trial registries and related websites, major conference proceedings, and field-related journals from January 1, 1980, to July 1, 2020, for studies comparing RIC with MAC before the first allo-HSCT in patients with AML in CR or MDS. Only randomized controlled trials (RCTs) were included. OS was the primary endpoint and generic inverse variance method was used to combine hazard ratio (HR) and 95% CI.ResultsWe retrieved 7,770 records. Six RCTs with 1,413 participants (711 in RIC, 702 in MAC) were included. RIC had the same OS (HR = 0.95, 95% CI 0.64–1.4, p = 0.80) and cumulative incidence of relapse as MAC (HR = 1.18, 95% CI 0.88–1.59, p = 0.28). Furthermore, RIC significantly reduced non-relapse mortality more than total body irradiation/busulfan-based MAC (HR = 0.53, 95% CI 0.36–0.80, p = 0.002) and had similar long-term OS and graft failure as MAC.ConclusionRIC conditioning regimens are recommended as an adequate option of preparative treatment before allo-HSCT for patients with AML in CR or MDS.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=185436.

The outbreak of COVID-19 pandemic has caused profound consequences on world economy. In order to explore the long-term impact of the pandemic on economic growth and the effects of different policy responses, this paper combines economic theory with epidemiological model to construct an interdisciplinary model, in which labor supply is dynamically constrained by pandemic conditions. Analysis of model equilibrium suggests that outbreaks of infectious disease reduce labor supply and negatively affect economic output. The accumulation of health capital can suppress the spread of disease and improve the recovery rate of infected individuals, which will alleviate the labor supply constraint caused by the pandemic and lead to an increase in output and consumption. The model is then calibrated to Chinese economy. The simulation results imply that government's public health policy can enhance the role of health capital in promoting economic growth. But the marginal effect of certain policies is diminishing. Therefore, the government needs to balance pandemic prevention and control costs and marginal benefits when formulating public health policies. When the pandemic is under control, the resumption of production is feasible and the economic stimulus package could lead to economic recovery.

This paper constructs a partial equilibrium model under public health emergency shocks based on economic growth theory, and investigates the relationship between government intervention and virus transmission and economic growth path. We found that both close contacts tracing measures and isolation measures are beneficial to human capital stock and economic output per capita, and the effect of close contact tracing measures is better than that of isolation measures. For infectious diseases of different intensities, economic growth pathways differed across interventions. For low contagious public health emergencies, the focus should be on the coordination of isolation and tracing measures. For highly contagious public health emergencies, strict isolation, and tracing measures have limited effect in repairing the negative economic impact of the outbreak. The theoretical model provides a basic paradigm for the future researches to study economic growth under health emergencies, with good scalability and robustness.

Abstract Location-based recommendation services can provide users with convenient services, but this requires monitoring and collecting a large amount of location information. In order to prevent location information from being leaked after monitoring and collection, location privacy must be effectively protected. Therefore, this paper proposes a privacy protection method based on location sensitivity for location recommendation. This method uses location trajectories and check-in frequencies to set a threshold so as to classify location sensitivity levels. The corresponding privacy budget is then assigned based on the sensitivity to add Laplace noise that satisfies the differential privacy. Experimental results show that this method can effectively protect the user’s location privacy and reduce the impact of differential privacy noise on service quality.

Carbon emission leads to environmental and social consequences, which could be severe in the emerging economies. Owing to the dilemma of emission and economic expansion, it is necessary to achieve a more comprehensive understanding of the dynamic relationship between economic growth and carbon emission. Multivariate Wavelet analysis is introduced in addition to the decoupling analysis for BRICS countries. The decoupling analysis detects an obvious trend of economic growth decoupling from carbon emission in China, and generates mixed results for the other countries. Estimates of wavelet coherency suggest that BRICS countries have experienced different kinds of structural changes in growth–emission nexus. Results of partial phase-difference and wavelet gain imply that different resource endowments and growth paths lead to varied impact of economic growth on carbon emission and time-varying characteristics of the causality relationship over different frequencies. Energy structure and trade openness matter for anatomizing this time-varying relationship. To succeed in the fight against climate change, the policy makers need to pay serious attention to the dynamic impact of economic growth, energy structure, and trade openness on carbon emission.

Mung bean (Vigna radiata L.) is an important edible bean in the human diet worldwide. However, its growth, development, and yield may be restricted or limited by insufficient or unbalanced nitrogen (N), phosphorus (P), and potassium (K) fertilization. Despite this, there are few long-term studies of the effects of varying levels of N, P, and K combined fertilizers and the optimal fertilization for improving mung bean yield and quality. This study was conducted to optimize the fertilization strategies for high yield and to improve yield components (pods per plant, seeds per pod, and 100-seed weight) in the Bailv9 mung bean cultivar, 23 treatments were tested in 2013-2015, using a three-factor (N, P, and K fertilizers), five-level quadratic orthogonal rotation combination design. Our studies showed that, the N, P, and K fertilizers significantly influenced the pods per plant and yield, which increased and then decreased with the increasing N, P, and K fertilizers. The 100-seed weight was significantly affected by the N and P fertilization, and it was increased consistently with the increasing N fertilizer, and decreased significantly with the increasing P fertilizer. Whereas, the seeds per pod significantly decreased with the increasing N and K fertilizers, and the P fertilizer had no significant effect on it. The NP interaction had a significant effect on yield and pods per plant at high N levels, while the NK interaction had a significant but opposite effect on yield at low N levels. The optimal fertilization conditions to obtain yield >2,141.69 kg ha-1 were 34.38-42.62 kg ha-1 N, 17.55-21.70 kg ha-1 P2O5, and 53.23-67.29 kg ha-1 K2O. Moreover, the optimal N, P, and K fertilization interval to achieve pods per plant > 23.41 and the optimal N fertilization to achieve a 100-seed weight > 6.58 g intersected with the interval for yield, but the seeds per pod did not. The fertilizer ratio for the maximum yield was N:P2O5:K2O = 1:0.5:1.59. Following three years experimentation, the optimal fertilization measures were validated in 2016-2017, the results indicated that yield increased by 19.6% than that obtained using conventional fertilization. The results of this study provide a theoretical basis and technical guidance for high-yield mung bean cultivation using the optimal fertilization measures.

The adzuki bean (Vigna angularis) is an important grain legume. Fine mapping of quantitative trait loci (QTL) and qualitative trait genes plays an important role in gene cloning, molecular-marker-assisted selection (MAS), and trait improvement. However, the genetic control of agronomic traits in the adzuki bean remains poorly understood. Single-nucleotide polymorphisms (SNPs) are invaluable in the construction of high-density genetic maps. We mapped 26 agronomic QTLs and five qualitative trait genes related to pigmentation using 1,571 polymorphic SNP markers from the adzuki bean genome via restriction-site-associated DNA sequencing of 150 members of an F2 population derived from a cross between cultivated and wild adzuki beans. We mapped 11 QTLs for flowering time and pod maturity on chromosomes 4, 7, and 10. Six 100-seed weight (SD100WT) QTLs were detected. Two major flowering time QTLs were located on chromosome 4, firstly VaFld4.1 (PEVs 71.3%), co-segregating with SNP marker s690-144110, and VaFld4.2 (PEVs 67.6%) at a 0.974 cM genetic distance from the SNP marker s165-116310. Three QTLs for seed number per pod (Snp3.1, Snp3.2, and Snp4.1) were mapped on chromosomes 3 and 4. One QTL VaSdt4.1 of seed thickness (SDT) and three QTLs for branch number on the main stem were detected on chromosome 4. QTLs for maximum leaf width (LFMW) and stem internode length were mapped to chromosomes 2 and 9, respectively. Trait genes controlling the color of the seed coat, pod, stem and flower were mapped to chromosomes 3 and 1. Three candidate genes, VaAGL, VaPhyE, and VaAP2, were identified for flowering time and pod maturity. VaAGL encodes an agamous-like MADS-box protein of 379 amino acids. VaPhyE encodes a phytochrome E protein of 1,121 amino acids. Four phytochrome genes (VaPhyA1, VaPhyA2, VaPhyB, and VaPhyE) were identified in the adzuki bean genome. We found candidate genes VaAP2/ERF.81 and VaAP2/ERF.82 of SD100WT, VaAP2-s4 of SDT, and VaAP2/ERF.86 of LFMW. A candidate gene VaUGT related to black seed coat color was identified. These mapped QTL and qualitative trait genes provide information helpful for future adzuki bean candidate gene cloning and MAS breeding to improve cultivars with desirable growth periods, yields, and seed coat color types.

The quick emergence of the COVID-19 pandemic has shaken the foundations of the world's economy. Notably, women entrepreneurs were significantly in danger of income and sales due to the COVID-19 pandemic, which has had significant economic and social impacts. This study examines the effects of the COVID-19 pandemic Standard Operating Procedures (SOPs) on women entrepreneurs' sales performance, engaged in home-based activities. The chi-square test of association and linear probability model with interactions were applied to analyze the data collected using a questionnaire from 672 women entrepreneurs. Generally, cloth and cosmetics entrepreneurs reported an increase in sales. However, following the COVID-19 pandemic SOPs negatively affected women's entrepreneurial sales performance. Variations in findings were observed by testing the interaction and heterogeneous effects. The household contextual analysis is crucial for understanding the dynamics of women home-based entrepreneurs in the family and cultural context as they reported an increase in sales volume. The study's findings point to the adaptability, resilience, and multiplier effects of women entrepreneurs who faced economic, sociocultural, and institutional restraints encountered during the uncertain period of the COVID-19 pandemic. [ABSTRACT FROM AUTHOR]