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Maintaining proteostasis in the endoplasmic reticulum (ER) is critical for cell viability and plant survival under adverse conditions. The unfolded protein response (UPR) pathways interact with reactive oxygen species (ROS) to precisely trigger adaptive outputs or cell death under ER stress with varying degrees. However, little information is known about the relationship between UPR signalling and ROS regulation. Here, Arabidopsis GOLGI ANTI-APOPTOTIC PROTEIN1 (GAAP1)-GAAP4 were found to play redundant positive roles under ER stress. Genetic analysis showed that GAAP4 played a role in INOSITOL-REQUIRING ENZYME (IRE1)-dependent and -independent pathways. In addition, GAAPs played negative roles to activate the adaptive UPR under conditions of stress. Quantitative biochemical analysis showed that mutations in GAAP genes decreased the oxidised glutathione content and altered the pattern of ROS and glutathione in early ER stress. When plants were challenged with unmitigated ER stress, mutations in GAAP advanced ROS accumulation, which was associated with a decline in adaptive UPR. These data indicated that GAAPs resist cell death by regulating glutathione content to inhibit ROS accumulation and maintain UPR during ER stress. They provide a basis for further analysis of the regulation of cell fate decision under ER stress.

More and more research had focused on the osteogenesis of demineralized dentin in clinic, especially when the first application of deminerized dentin in 2008. The study tried to compare the osteogenetic ability of the demineralized dentin block, which were processed two different regents by VacuaSonic system. The extracted human permanent teeth were demineralized by two different methods. Then the MC3T3-E1 cells were invited to culture on the surface of these demineralized dentin blocks (DDB). The cell attachment, proliferation and differentiation were tested. Adhesion of MC3T3-E1 on DDM was observed using scanning electron microscopy and confocal test, the Alizarin Red S, ALP activity, and the protein of BMP-2/-7 and OCN were employed to confirm the level of cell differentiation. The P value was set at 0.05. The microfilaments established a good contact and formed a network in Group A. The Group A had more full cytoskeleton and actin stretched more obviously than Group C, the number of cells on three scaffolds were difference (p < 0 05). The MTT results showed no cytotoxicity in all experiment groups, and Group C had a significant difference in cell proliferation than other groups (p < 0 05) except for day 1. While when related to the cell differentiation, Group A showed a similar result with Group C, but in Alizarin Red S, Group A had a superior result (p < 0 05). The tooth dentin scaffold processed with composite acids in Group A presents the superiority in osteoconduction and preferable osteogenesis ability, which could be an alternative method to process the tooth scaffold.

Previously, a number of previous studies on human leukocyte antigen G (HLA-G) and preeclampsia (PE) have demonstrated that expression of HLA-G is significantly reduced in women with PE. However, no study has confirmed whether maternal serum HLA-G could be used as a clinical test when HLA-G1/-G5 isoforms were measured. Therefore, the present study is to develop a novel HLA-G ELISA which is able to detect all isoforms of HLA-G and then to perform a retrospective case-control study to investigate clinical significance of maternal serum HLA-G for predicting PE.A recombinant HLA-G fragment which containing partial sequences of HLA-G α1 and α2 domains was constructed to develop two novel monoclonal antibodies against HLA-G. A novel HLA-G sandwich ELISA which could detect all isoforms of HLA-G was developed. By using the ELISA, predictive effectiveness of maternal serum HLA-G in a retrospective case control study was evaluated.At the first trimester and early second trimester, detection of maternal serum HLA-G had the sensitivity of 54.3% and 48.5% and the specificity of 97.8% and 96.3% in the prediction of PE. These were significantly higher than those at the third trimester (P 0.05).HLA-G isoforms other than HLA-G1/-G5 are expressed in some pregnant women who have low level or lack HLA-G1/-G5. Measurement of all HLA-G isoforms in maternal serum could be used as a clinical test for early prediction of PE.

Background: Human leukocyte antigen G (HLA-G), a non-classic class I antigen, has been well documented in that its concentrations in maternal peripheral blood are significantly lower in pregnant women with preeclampsia (PE) than those pregnant women without PE. However, the clinical significance of measuring serum HLA-G as a test for predicting PE development has not been studied. Methods: First, we determined cutoff values for serum HLA-G levels for PE in a retrospective case-control study with 100 PE patients and 300 non-PE pregnant women. Then, we performed a prospective unselected cohort study with 3,439 pregnant women. In the prospective study, pregnant women whose serum HLA-G levels were lower than the cutoff values and who had other PE risk factors were included in a PE rule-in group. In contrast, pregnant women whose serum HLA-G levels were greater than the cutoff values and who had no other PE risk factors were included in a PE rule-out group. The participants were followed up until end of study. Finding: The cutoff values of serum HLA-G levels for PE were established in the retrospective study. In the subsequent prospective study, we demonstrated that serum HLA-G levels lower than 80 U/mL at 10−13 gestational weeks (GW) in the PE rule-in group showed a positive predictive value for PE development with 83.78% sensitivity and 87.93% specificity while serum HLA-G levels higher than 80 U/mL at 10−13 GW in the PE rule-out group had a negative predictive value with 88.57% sensitivity and 97.14% specificity. Interpretation: Serum HLA-G levels lower than 80 U/mL in the first trimester of pregnancy can be used to predict the absence or presence of PE development in the third trimester of pregnancy. Funding Statement: Allowance to SMY for new technique development from provincial and municipal government department of Sichuan, Chengdu, P. R. China. Declaration of Interests: All authors declare no competing interests. Ethics Approval Statement: The hospital review board (Second Affiliated Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, P.R. China) approved the study protocol. All participants provided their written informed consent.

Background Congenital thrombotic thrombocytopenic purpura (TTP) is rare and is prone to misdiagnosis or missed diagnosis in clinical. The relationship between genotype and phenotype needs further study. Methods A 15-hour-old Chinese girl develops jaundice. Her platelet counts suddenly decreases with bleeding spots on the left side of chest, upper abdomen, and bilateral groin on the fourth day after birth. The plasma ADAMTS13 activity and inhibitor are detected by residual collagen binding assay. ADAMTS 13 gene is detected by next generation sequencing. Results The plasma ADAMTS13 activity of the patient is shown to be severely deficient, but without inhibitor. Gene sequencing analysis shows that the patient carries a compound heterozygote mutation of ADAMTS13 gene, one is c.1564T>C, p.(Cys522Arg) on exon 13 of the ADAMTS13 gene, a heterozygote missense mutation. It is identified as a de novo suspected pathological variation. The other is c.330+1G>A on intron 3 of the ADAMTS13 gene, a heterozygote splicing mutation. Her father and elder sister carry c.1564T>C, p.(Cys522Arg) on exon 13 of the ADAMTS13 gene, a heterozygote missense mutations. Her mother carries c.330+1G>A on intron 3 of the ADAMTS13 gene, a heterozygote splicing mutation. Conclusions The deficiency of ADAMTS13 caused by one heterozygote missense mutation and the other heterozygote splicing mutation are responsible for the episode of this congenital TTP patient.

This paper investigates the unsteady structures and the hydrodynamics of cavitating flows. Experimental results are presented for a Clark-Y hydrofoil fixed at α = 0°, 5° and 8°, for various cavitation numbers, from subcavitating flow to supercavitation. The high-speed video camera and the particle image velocimetry (PIV) are applied to observe the transient flow structures. Statistics of the cavity lengths, the velocity and vorticity distributions, as well as the turbulent intensities are presented to quantify the unsteady process. Meanwhile, the dynamic measurement system is used to record the dynamic characteristics. The experimental results show that the flow structures and the hydrodynamics of the cavitation vary considerably with various combinations of angles of attack and cavitation numbers. Under various conditions, the cavitation can be generally grouped as the inception cavitation, the sheet cavitation, the cloud cavitation and the supercavitation. The cloud cavitation exhibits noticeable unsteady characteristics. Experimental evidence indicates that the hydrodynamics are clearly affected by the cavitating flow structures, the amplitude of the load fluctuation is much higher in the cloud cavitating cases.

A series of CeO2 nanocrystals with different sizes have been synthesized with DNA as the template by a hydrothermal synthesis method. The peroxidase-like activity of the CeO2 nanocrystals was found to be strongly enhanced by DNA doping.

ASTC (allatostatin C) and ASTCC (allatostatin double C) are two neuropeptide genes that encode allatostatin C-like peptides. Whether these peptides inhibit or have other effects on juvenile hormone (JH) synthesis in the corpora allata remains in question. The juvenile hormone acid O-methyltransferase (JHAMT), a key gene in the JH biosynthesis pathway, was selected to study the effects of ASTC and ASTCC on juvenile hormones. In this study, we first characterized the expression patterns and correlations between ASTC and ASTCC in Clostera anastomosis under natural conditions, and then the functions of these two genes were investigated by RNAi. The results showed the expression of JHAMT was strongly positive correlated with ASTC and ASTCC in the heads of larvae after ASTC and ASTCC were knocked down simultaneously, while negative correlations were found in the heads of female adults. These results suggest that both ASTC and ASTCC may stimulate JH biosynthesis in larvae while inhibit in female adults of C. anastomosis. And after either ASTC or ASTCC was knocked down alone, the correlation between the two genes were positive, indicating ASTC and ASTCC may function dependently in female heads. In addition, simultaneous suppression of ASTC and ASTCC increased the mortalities of larvae and pupae.

The goat genome is the research basis for the protection and utilization of goat resources, which is important for breeding and improving goat breeds. At present, with the continuous improvement of goat reference genome, various important research progress in goat origin, evolution and adaptability has been achieved. In this review, we summarize the research progress in the goat genome in detail, encompassing goat genome structure, genome map (genetic, physical and comparative maps), goat high throughput sequencing and SNP chip development. We aim to provide a theoretical foundation for the development of goat genome selection.

Background Primary neonatal hypocholinesterase is rare; its genetic pattern and mutation still need to be further studied. Methods The patient and his parents are studied using next-generation sequencing technology. Results A boy one day after birth is admitted to the Neonatal Intensive Care Unit at our hospital after experiencing intermittent vomiting for 12 hours. The patient's serum cholinesterase level (113 - 283 U/L) is lower than normal value (4,000 - 12,600 U/L). Many factors of low serum cholinesterase are excluded. We highly suspect that it may be related to congenital factors. Molecular genetic test results show that the patient carried the BCHE gene (NM_000055.2) and has homozygous frameshift mutations at exon 2 c.401dupA (p.Asn134fs) of chromosome 3q26. It is a pathogenicity mutation. This locus mutation belongs to a novel pathogenic mutation. As a result of this mutation, the 134th amino acid Asn began to frameshift and the translation is terminated early. It can cause the Encoding of protein to truncate and lose its normal function. His parents' serum cholinesterase levels (father: 5,135 U/L; mother: 4,367 U/L) are in the normal value range, but his parents carried a heterozygous BCHE gene. Conclusions This study suggests that gene sequence detection should be carried out early in hypocholinesterase of nknown cause in neonates. This study can not only improve understanding of the etiology and pathological mechanism of hypocholinesterase, but also it can enlarge the hypocholinesterase gene mutation spectrum.

The thermosensitive hydrogel was prepared by embedding β-cyclodextrin into chitosan/αβ-glycerol phosphate system. The optimal parameters were that the ratio of CS/β-CD was 2/1 (w/w) and the ratio of CS-β-CD/αβ-GP was 9.0/1.0 (v/v), where the gelation time was less than 3 min. The hydrogels had obvious lamellar structure, self-comparability, fractal characteristic and had good sustained release efficiency on the hydrophobic drug. Furthermore, the release rate of CS-β-CD-In hydrogel was slower than that of CS-β-CD hydrogel which indicated that the model drug (Asprin), added as Asp-β-CD inclusion, was beneficial for sustaining the release from the modified hydrogel system. The analysis of release kinetics indicated that both CS-β-CD based hydrogel and CS-β-CD-In hydrogel showed best fit with Peppas model and had potential as sustained release drug delivery system.

Cassandra database system is one of the universal databases. To achieve high performance, we should allocate memory space rationally according to actual demands. Otherwise, it will influence reading and writing performance. Actually, we always allocate memory space according to experience and repeated attempts which usually won’t give us the best answer. To solve this problem, firstly we analyze the reading and writing processing of the Cassandra database and find out the corresponding memory space which will influence system performance. Secondly, we build up a relationship model between system performance and memory allocation and name it as The Memory Model of Reading and Writing Performance. We have already applied the relationship model to real database servers to guide memory allocation and performance evaluation. Simulation results show that this memory model could well describe the quantization relationship of memory space and system performance.

Here, time-decoupled comprehensive two-dimensional ultra-high liquid chromatography (UHPLC) coupled with an ion mobility (IM)-high resolution mass spectrometer (HRMS) was established and used to analyze ginsenosides from the main roots of white ginseng (WG) and red ginseng (RG), which enabled the separation of complex samples in four dimensions (2D-LC, ion mobility, and mass spectrometry). The incompatibility of mobile phases, dilution effect, and long analysis time, which are the main shortcomings of traditional comprehensive 2D-LC methods, were largely avoided in this newly established 2D-UHPLC method. The orthogonality of this system was 55%, and the peak capacity was 4392. Under the optimized 2D-UHPLC-IM-MS method, 201 ginsenosides were detected from white and red ginseng samples. Among them, 10 pairs of co-eluting isobaric ginseng saponins that were not resolved by 2D-UHPLC-HRMS were further resolved using 2D-UHPLC-IM-MS. In addition, 24 ginsenoside references were analyzed by UHPLC-IM-MS to obtain their collision cross section (CCS) values and ion mobility characteristics. Finally, the established new method combined with multivariate statistical analysis was successfully applied to differentiate WG and RG, and 9 ginsenosides were found to be the potential biomarkers by S-Plot and the values of max fold change, which could be used for classifying WG and RG samples. Overall, the obtained results demonstrate the applicability and potential of the established time-decoupled online comprehensive 2D-UHPLC-IM-MS system, and it will be extended to the analysis of other targeted or untargeted compounds, especially co-eluting isomers in more herbal extracts.

The aim of this study was to discuss the feasibility of porous mineralized collagen plug and bilayer mineralized collagen-guided bone regeneration membrane in site preservation in extraction sockets. The third mandibular premolars on both sides were extracted from four dogs, thus there were 16 alveolar sockets in all dogs and were randomly assigned into three groups. Group A had six alveolar sockets, and groups B and C had five alveolar sockets, respectively. Each alveolar socket of group A was immediately implanted with a porous mineralized collagen plug and covered with a bilayer mineralized collagen-guided bone regeneration membrane after tooth extraction. Alveolar sockets of group B were implanted with porous mineralized collagen plug only, and group C was set as blank control without any implantation. The healing effects of the extraction sockets were evaluated by gross observation, morphological measurements, and X-ray micro-computed tomography after twelve weeks. Twelve weeks after operation, both groups A and B had more amount of new bone formation compared with group C; in terms of the degree of alveolar bone height, group A was lower than groups B and C with significant differences; the bone mineral density in the region of interest and bone remodeling degree in group A were higher than those of groups B and C. As a result, porous mineralized collagen plug could induce the regeneration of new bone in extraction socket, and combined use of porous mineralized collagen plug and bilayer mineralized collagen guided bone regeneration membrane could further reduce the absorption of alveolar ridge and preserve the socket site.

Although hypothermia therapy is effective to treat neonatal hypoxic-ischemic encephalopathy, many neonatal patients die or suffer from severe neurological dysfunction. Erythropoietin is considered one of the most promising neuroprotective agents. We hypothesized that erythropoietin combined with hypothermia will improve efficacy of neonatal hypoxic-ischemic encephalopathy treatment. In this study, 41 neonates with moderate/severe hypoxic-ischemic encephalopathy were randomly divided into a control group (hypothermia alone for 72 hours, n = 20) and erythropoietin group (hypothermia + erythropoietin 200 IU/kg for 10 days, n = 21). Our results show that compared with the control group, serum tau protein levels were lower and neonatal behavioral neurological assessment scores higher in the erythropoietin group at 8 and 12 days. However, neurodevelopmental outcome was similar between the two groups at 9 months of age. These findings suggest that erythropoietin combined with hypothermia reduces serum tau protein levels and improves neonatal behavioral neurology outcome but does not affect long-term neurodevelopmental outcome.

Modern software systems are growing ever more complex, and the component-based software engineering (CBSE) is viewed as an opportunity to deal with the increasing complexity of measurement and control system. This paper analyzes the task profile of measurement and control system (GMCS) and discusses the component-based design methods. The paper submits a novel model called CV-MDN for software of GMCS that includes five parts: user controller, display views, domain model, data services and network service. The five parts provide cooperation method with software buses. Finally, the paper introduces the architecture of software design and it analyzes the software system functions. The CV-MDN model can be formed a general method for design of GMCS.

With the development of gene chip and breeding technology, genomic selection in plants and animals has become research hotspots in recent years. Genomic selection has been extensively applied to all kinds of economic livestock, due to its high accuracy, short generation intervals and low breeding costs. In this review, we summarize genotyping technology and the methods for genomic breeding value estimation, the latter including the least square method, RR-BLUP, GBLUP, ssGBLUP, BayesA and BayesB. We also cover basic principles of genomic selection and compare their genetic marker ranges, genomic selection accuracy and operational speed. In addition, we list common indicators, methods and influencing factors that are related to genomic selection accuracy. Lastly, we discuss latest applications and the current problems of genomic selection at home and abroad. Importantly, we envision future status of genomic selection research, including multi-trait and multi-population genomic selection, as well as impact of whole genome sequencing and dominant effects on genomic selection. This review will provide some venues for other breeders to further understand genome selection.

Introduction The Kham Tibetans are one of several Tibetan ethnic subgroups living in the Kham area of China. Because studies on the high-altitude adaptation of the Kham people are scant, the main aim of this study is to investigate whether the response to hypoxia, especially polycythemia status, in the Kham Tibetans is different from other Tibetan ethnic subgroups. Methods The primary investigation was conducted on 346 native Kham Tibetan adults (268 men and 78 women) from 3 herdsmen villages located in Hongyuan County situated at an altitude of greater than 3600 m. The participants were aged 46.2±14.1 (21–82; mean±SD with range) years. Anthropometric measurements such as weight, height, waist circumference, body mass index, and blood pressure, as well as laboratory blood tests such as glycosylated hemoglobin, hemoglobin, total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and uric acid were analyzed. Results The concentrations of hemoglobin were 171.3±12.9 (66–229) mg·L -1 and 151.4±16.4 (86–190) mg·L -1 in men and women, respectively. The frequency of polycythemia was found to be 25.5 and 21.8% in men and women, respectively. Polycythemia was found to be significantly associated with glycosylated hemoglobin concentrations, hypertension, and hyperuricemia ( P =0.002, 0.023, and 0.009, respectively). Conclusions There is a higher frequency of polycythemia in the Kham Tibetans when compared with reported studies from other Tibetan ethnic subgroups living on the Qinghai-Tibet plateau.

Novel ethyl cellulose/chitosan microspheres (ECCMs) were prepared by the method of w/o/w emulsion and solvent evaporation. The microspheres were spherical, adhesive, and aggregated loosely with a size not bigger than 5 μm. The drug loading efficiency of berberine hydrochloride (BH) loaded in microspheres were affected by chitosan (CS) concentration, EC concentration and the volume ratio of V(CS)/V(EC). ECCMs prepared had sustained release efficiency on BH which was changed with different preparation parameters. In addition, the pH value of release media had obvious effect on the release character of ECCMs. The release rate of BH from sample B was only a little more than 30% in diluted hydrochloric acid (dHCl) and that was almost 90% in PBS during 24 h. Furthermore, the drug release data were fitted to different kinetic models to analyze the release kinetics and the mechanism from the microspheres. The released results of BH indicated that ECCMs exhibited non-Fickian diffusion mechanism in dHCl and diffusion-controlled drug release based on Fickian diffusion in PBS. So the ECCMs might be an ideal sustained release system especially in dHCl and the drug release was governed by both diffusion of the drug and dissolution of the polymeric network.

Thread level speculation has been proposed and researched to parallelize traditional sequential applications on homogeneous multi-core architecture. In this paper, a heterogeneous multi-core hardware simulation system is present, which provides with TLS execution mechanism. With a novel TLS programming model and a number of new speculative tuning techniques, benchmarkGzipis parallelized from-3% to 195% on a four-core processor, and the speedup of the test benchmarks are 30%, 43% and 156%, respectively with arbitrary, hotspot and insight speculation.

Abnormal aggregation of α-synuclein (α-syn) is central to the pathogenesis of Parkinson's disease (PD). Histone deacetylase 6 (HDAC6) was previously shown to control major cell response pathways to the cytotoxic ubiquitinated aggregates in some protein aggregation diseases. Whether it influences the aggregation process of α-syn in PD models and its related mechanisms are not completely known. Here, we characterized the expression and function of HDAC6 in the ubiquitin-proteasome system impairment-induced PD model. Our results showed that HDAC6 inhibition further exacerbated the nigrostriatal dopamine neurodegeneration and upregulated α-syn oligomers levels, whereas HDAC6 overexpression in vitro showed the opposite effects. More importantly, we provided evidence for the first time that HDAC6 regulating α-syn oligomers levels were related to its ability to trigger the heat shock response in a heat shock protein 90-dependent manner. HDAC6 mediated the dissociation of heat shock protein 90-heat shock factor 1-containing complex, and the activation of heat shock factor 1, which led to the expression of major molecular chaperones to prevent the deleterious α-syn aggregation. Thus, we propose that HDAC6 appears as a key modulator of cell protective response to the cytotoxic α-syn aggregates and may serve as a potential target for therapy development in PD.

the processing capacity of a server is unable to satisfy the parallel processing of mass data and also improves data transmission in storage system. This paper has adopted active storage system to transfer calculation task and management ability to data end, improving the parallel processing capability of application program and reducing the data transmission rate in the system. During processing arriving process, this research has complied with Poisson flow, and the service time for each processor is negative exponential distribution and task service rule is the mixing model of “first come, first served”. Through experiment, it is proved that the data transmission rate within MMC system is reduced by 15% than random allocation of task on average and the system speed-up ratio is improved by 2.1 than average.

Along with the developing of information technology, Java platform a plays more and more important role in building enterprise information system. Especially after the cloud computing emerged, Java applications are used as kinds of services to provide the users some function. Users use these services remotely, and the applications should give the users some trust evidence. Control-flow integrity endows the software the ability to show the applications' behaviors conform to the users' expectations. But the Java virtual machine's memory organization is different from physical machine and hinders the application of CFI. This paper gives out the a novel way to enforce the control flow integrity to the Java applications based on the memory organization of the Java virtual machine. The method presented in this paper can provide the user information about an applications behavior, and significantly improve the security of a Java application.

Phosphate ore was decomposed by using thermogravimetric (TG) and differential scanning calorimetric (DSC). The TG-DSC curves contained different sizes and heating rates. The results showed that phosphate ore had four weight loss stages at the range of 40 ~ 1100°C. With the particle size decreasing, the third weight loss stage became more obvious in TG curves and the endothermic peak became sharper in DSC curve ; the starting and ending temperature of thermal decomposition process moved to higher,the weight loss rate also tended to increase; the activation energy of phosphate ore endothermic decomposition process was calculated by Owaza method in 600~800°C, the average activation energy was 202.80 kJ / mol. It provided a basis for the utilizing of phosphate ore.

This paper gives out a novel way, TCFI4J, to enforce the control flow integrity to the Java applications based on Java virtual machine. TCFI4J combines the trusted computing technology and Java virtual machine together. It takes full advantage of the Trusted Platform Module (TPM) and gives full consideration to the memory organization of the JVM. TCFI4J takes the integrity of part of JVMs memory image into account for the control flow integrity enforcement. The method presented in this paper can provide the user information about an applications behavior. It can significantly improve the security of a Java application with a tolerable performance impact.

typically, integrity measurement technology for Java application is based on the whole file, which is too coarse to meet different requirements. In this paper, we propose a novel fine-grained integrity measurement method for Java application: trusted class measurement module (TCMM), which is a class integrity measurement method according to organization structure of Java application file. It not only satisfies necessary trust but also has less impact on system performance.

The article totally discusses the influence of polytetrafluoroethylene (PTFE) to the property of room temperature vulcanization silicone rubber (RTV) pollution flashover coating, inclduding hydrophobic, corrosion resistance and heat resistance. It is turned out that the PTFE can effectively improve the corrosion resistance of the coating only if the contents of PTFE has no impact on the mechanical property, flame retaradant and heat resisting property of the coating and highly enhances the hydrophobicity property.

Recent studies have demonstrated that human leukocyte antigen G (HLA-G) may play an important role in autoimmune diseases. The present study is to investigate whether or not HLA-G is associated with sacroiliitis stages of ankylosing spondylitis (AS), a systemic autoimmune disease. Plasma levels of soluble HLA-G (sHLA-G) and HLA-G expression on the surface of peripheral blood mononuclear cells (PBMCs) were measured in 55 AS patients and 49 healthy controls by using a specific HLA-G ELISA and flow cytometric (FCM) analysis, respectively. Association of HLA-G expression with sacroiliitis stages of the patients was statistically analyzed. The plasma sHLA-G concentrations were noticeably lower in the AS patients when compared to the healthy controls while the mean fluorescence intensity (MFI) of the HLA-G expression on the surface of PBMCs was significantly higher in the AS patients than in the healthy controls (both P

With the development of industrial production and the improvement people's living standards, all kinds of discharged wastewater cause serious pollution for nitrogen and phosphorus.In this study, the water of Dianchi lake was treated by Ultrasonic – flocculation. The results show ultrasound-flocculation sedimentation is better than only flocculation sedimentation in removing the total phosphorus, total nitrogen and reducing COD, BOD and turbidity respectively increased 16.19%, 12.71%, 9.1%, 20%, 18.3%.