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Objective. To investigate the combined application value of magnetic resonance imaging (MRI) combined with serum alpha-fetoprotein (AFP)-L3 and Golgi protein (GP)-73 in the diagnosis of primary liver cancer. Methods. The data of 200 patients with suspected liver cancer admitted to our hospital from February 2020 to February 2021 were retrospectively analyzed, and they were randomly divided into an experimental group and a control group, with 100 cases in each group. The experimental group received a combined detection of MRI with serum AFP-L3 and GP-73, and the control group adopted traditional diagnostic methods (spiral computed tomography and serum AFP). The diagnostic yields of the two groups were compared. Surgical resection was performed after the diagnosis of primary liver cancer, and the correlation between the efficacy and combined detection of MRI with serum AFP-L3 and GP-73 levels was analyzed. Results. The two groups presented comparable general information (P >0.05). The surgical results showed 160 cases of primary liver cancer, including 75 cases in the experimental group and 85 cases in the control group, and 40 cases of benign liver lesions. The diagnostic accuracy of the experimental group (73/75, 95%) was significantly higher than that of the control group (76/85, 86%) ( P < 0.05 ). The serum levels of AFP-L3, GP-73, and AFP in patients with primary liver cancer were remarkably decreased after surgery ( P < 0.001 ). The preoperative and postoperative AFP-L3, GP-73, and AFP levels of patients with primary liver cancer were significantly higher than those of patients with benign liver lesions. The AUC (95% CI) for the combined detection of MRI and serum AFP-L3 and GP-73 levels in patients with surgically confirmed primary liver cancer was 0.747 (0.619-0.874). Conclusion. MRI combined with serum AFP-L3 and GP-73 presents favorable diagnostic efficiency in the diagnosis of primary liver cancer, which is worthy of clinical application.

Drowning is a leading cause of accidental injury in children and has a great impact on family and society. The prevention and treatment of drowning is of great importance for reducing mortality rate. This consensus reviews the literature on the epidemiology, rescue, resuscitation, and acute clinical management and prevention of drowning. The panel determines the score of available evidence according to the criteria of Oxford Centre for Evidence-Based Medicine and then makes recommendations on evidence based on such criteria, so as to provide a basis for further reducing the mortality and disability rates caused by drowning.

Acid phosphatase (ACPase) is a common component in venom of parasitoids. Although extensive researches regarding this enzyme have been conducted in many other organisms, its characteristics as a venomous enzyme are still sparsely known. In this study, we aimed to reveal the gene expression patterns, and structural and biochemical properties of an ACPase from the venom of Scleroderma guani. The cloned open reading frame of venomous ACPase gene of S. guani was 1218 bp encoding 406 deduced amino acids, shared 40% and 41% identities to ACPases from venoms of Apis mellifera and Pteromalus puparum, respectively. The structural analysis of this ACPase implied common functions and differences to the honeybee venom ACPase. qPCR analysis showed that this gene was abundantly expressed in the venom apparatus, and most highly expressed in the adult stage after one and three days emergence. Activity assay using p-nitrophenyl phosphate as the substrate revealed that the optimal pH and temperature for this venomous enzyme was 4.8 and 45 °C, respectively. NaF is an effective inhibitor for it. The results will enrich our knowledge for the ACPase as toxin, which may contribute to further uncovering its role involved in parasitism.

This article introduces the Unified Theory of Concrete Filled Steel Tube (CFST) into counting strength of composite CFST columns subjected to axial compression. There were four types of composite CFST columns listed, and one equivalent confinement coefficient was proposed with consideration of different sections of confining materials, so the whole cross section can be taken as one new composite material to research axial compressive behaviors of the composite CFST column. One unified design formula was derived from the Unified Theory, which was suitable for solid or hollow, circular or non-circular various cross sections of the composite CFST columns. The ultimate compressive bearing capacity was calculated in comparison with corresponding test results. The results show that the equivalent confinement coefficient can be used to calculate the compressive strength of the composite CFST column, and it has a linear relation with the enhanced coefficient of composite strength. Thus, the equivalent con...

BACKGROUND:Recent studies have shown that extralevator abdominoperineal resection has the potential for reduced circumferential resection margin involvement, intraoperative bowl perforation, and local recurrence rates; however, it has been suggested that extended resection may be associated with inc

A quenched surface was applied in flow boiling, which is fabricated by quenching a piece of copper in carbon nanotube (CNT) fluid.Its performance was compared with bare copper, and the results showed that the quenched surface can enhance the heat flux by 15–20%. Quenched surface was less effective in enhancing heat flux as the flow rate and liquid subcooling was increased.

Despite substantial advances in uncovering constituents of parasitoid venoms due to their potential applications as insecticides and pharmaceuticals, most of these studies are primarily restricted to braconid and ichneumonid wasps. Little information is available regarding virulent factors from venom of Eulophidae. In order to provide insight into the venom components of this family and parasitoid venom evolution, a venom protein repertoire (venomics) of the endoparasitoid wasp, Tetrastichus brontispae was deciphered using a proteomic approach. A large number of diverse venom proteins/peptides were identified, including novel proteins and those proteins commonly found in the venoms of other parasitoids such as serine protease, esterase, dipeptidyl peptidase IV, acid phosphatase, major royal jelly protein, superoxide dismutase, and venom allergen 3/5. Three ion transport peptide-likes (ITPLs) were abundantly detected in T. brontispae venom. Of these, two of them are reported as a novel form for the first time, with the characteristics of lengthened amino acid sequences and additional cysteine residues. These venom ITPLs are obviously apart from other general members within the crustacean hyperglycemic hormone/ion transport peptide (CHH/ITP) family. It implies that they would evolve unique functions essential for parasitism success.

In this work, a systematic study was carried out on the cutting process of expendable pattern, and the impact of process parameters, such as spindle speed, feed rate and cutting depth on the dimensional accuracy of expendable pattern was discussed. The results show that the dimensional accuracy at length and width directions of expendable pattern is less affected by the cutting process, while the machining accuracy at height direction of expendable pattern is greatly influenced by the cutting process. With the increase of spindle speed, the height error reduced undulately; with the increase of feed rate, the height error increases gradually; the selection of cutting depth has a reasonable range, above or below this range will cause the increase of the dimension error.

According to the limited measurement range of specific coordinate measuring machine (CMM), a reverse test method was put forward, and an auxiliary measuring equipment for tiny hole was developed. A measuring method for tiny hole was formulated, which using gauge head trigger signal of CMM as the test signal. The principle which was the tiny hole moving but not moving gauge head was used and the corresponding measurement software was designed so that the small measurement range was expanded. The measurement precision influence factors were analyzed and the measurement scheme was optimized. The results indicate that: then the measurement range of tiny hole is expanded to 1.0mm based on the combination of auxiliary equipment and CMM. The precision error is 0.004 mm which meets the measurement requirements.

Background To analyze the causes and consequence of treatment refusal and abandonment in childhood acute lymphoblastic leukemia (ALL) treated in Wuhan Union Hospital of China. Methods We collected recorded data and interviewed families of the children with ALL diagnosed between January 1997 and August 2007, who refused or abandoned treatment. Results 323 patients were diagnosed with ALL. 173 patients (173/323, 53.6%) refused therapy and 35 (35/323, 10.8%) cases abandoned treatment. 191 (191/208, 91.8%) of these children were telephone/mail-visited. Different people had different reasons for refusal or abandonment. Financial difficulty and belief of ALL incurability were the main reasons for abandonment. Transportation difficulties and fear of severe side effects were also important reasons. Of the 173 patients who refused treatment, 13 patients lost follow-up. 160 parents were interviewed and 1 (6.3%) child was still alive at the date of last follow-up. Of the 35 patients who abandoned treatment, 4 patients lost follow-up. 31 parents were interviewed and 2 (6.5%) children were still alive at the date of last follow-up. Conclusion Medical insurance and a systemic health education are extremely required for childhood ALL in low middle income countries.

In order to offer theory base for the widely use of Roadbood EN-1 soil firming agent in road base, triaxial shearing tests are performed on road base consolidated with Roadbood EN-1 soil firming agent, cement and lime to analyze its mechanical properties. Stress-straining curve, peak strength, remnant strength, elastic modulus and shearing strength of road base consolidated with Roadbood EN-1 soil firming agent, cement and lime under different ambient pressures are measured through triaxial shearing test. The conclusions are peak strength, remnant strength and peak straining have straight line relationship with ambient pressure. Its destruction procedure can be explained with impairment. The stress-strain relation curve also indicated that Duncan-Chang Model could not be applied to road base consolidated with Roadbood EN-1 soil firming agent, cement and lime, while the modified Saenz Formula could be used to describe the stress-strain relation of it,and good agreement was obtained.

In imaging electron optics, for a concentric spherical system composed of two spherical electrodes with electrostatic focusing, the electrostatic potential distribution and the spatial–temporal trajectory of electron motion can be expressed by analytical forms. It is naturally to take such system as an ideal model to investigate the imaging properties, as well as the spatial–temporal aberrations, to analyze its particularity and to find the clue of universalities and regularities. Research on this problem has important significance, which can afford theoretical foundation not only in studying static electron optics for the night vision devices, but also in studying dynamic electron optics for high-speed image converter tubes. In the present paper, based on the practical electron ray equation and electron motion equation for a bi-electrode concentric spherical system with electrostatic focusing, the spatial–temporal trajectories of moving electrons emitted from the photocathode have been solved, the exact and approximate formulae for image position and flight time of electrons, have been deduced. Start from solutions of spatial–temporal trajectories, the electron optical spatial–temporal properties of this system are then discussed. According to the definitions of spatial–temporal aberrations, the paraxial and geometrical lateral aberrations, as well as the paraxial and geometrical temporal aberrations, have been deduced, that are classified by the order of e z / ϕ a c and e r / ϕ a c .

To study the clinical characteristics of ecotopic viral integration site-1 (EVI1) and BCR/ABL positive childhood leukemia.Clinical data of four children with EVI1 and BCR/ABL positive leukemia and eight children with BCR/ABL positive but EVI1 negative chronic myeloid leukemia (CML) were retrospectively analyzed.In the four children with EVI1 and BCR/ABL positive leukemia, two were initially diagnosed with chronic phase of CML, one with accelerated phase of CML and one with high-risk acute lymphoblastic leukemia (ALL). There were no significant differences in clinical characteristics at diagnosis between the patients with EVI1 and BCR/ABL positive leukemia and BCR/ABL positive but EVI1 negative leukemia. CD33 and CD38 were highly expressed and t(9;22) abnormality was present in all patients with EVI1 and BCR/ABL positive leukemia. Two of the 3 children with EVI1 and BCR/ABL positive CML achieved complete remission one or three months after treatment. Acquired negative status conversion occurred for EVI1 but not BCR/ABL in one CML case. The 3 children with EVI1 and BCR/ABL positive CML survived 20, 13 and 14 months, respectively, without recurrence. The child with EVI1 and BCR/ABL positive ALL failed to achieve complete remission after the first course of treatment and discontinued further treatment.Co-expression of EVI1 and BCR/ABL fusion gene can be found in childhood CML and ALL. The relatively rare leukemia has not significant difference respect to clinical characteristics. Prognosis of the disease needs to be determined by clinical studies with a larger sample size.

To study the expression of ecotropic viral integration site (EVI1) gene in childhood acute myeloid leukemia (AML) and the clinical features of EVI1-positive children with AML.The clinical data of EVI1-positive children with AML were collected and analyzed. RT-PCR and real-time quantitative PCR were used for qualitative and quantitative analysis of expression of EVI1. Flow cytometry (FCM) was used for determining the immunophenotypes of bone marrow cells. Multiparameter FCM was used for monitoring minimal residual disease. The karyotypes were determined.Of 241 children with AML, 33 (13.7%) were positive for EVI1 expression. There were no significant differences in age at first visit as well as the white blood cell count, hemoglobin level, and platelet count in peripheral blood between EVI1-positive and EVI1-negative children with AML (P0.05), but EVI1-positive children had a significantly increased proportion of females compared with EVI1-negative children (P0.05). The change in EVI1 expression was not synchronous with clinical remission and the change of MRD: some children had clinical remission or negative conversion of MRD before negative conversion of EVI1, while some had negative conversion of EVI1 before clinical remission or while MRD showed positive. EVI1 gene was usually co-expressed with other fusion genes. CD33 (100%), CD38 (88%), and HLADR (76%) were highly expressed in EVI1-positive children with AML. Abnormal chromosome structure or number was found in 15 patients. Compared with EVI1-negative children, EVI1-positive children had significantly lower complete remission rates after the first course of treatment (P0.05).EVI1-positive children with AML have a poor short-term prognosis. In the development of AML, the activation of EVI1 gene is not isolated, but the result of interactions with other genes or chromosome abnormalities, and the mechanism of activation and its function need further study.

Although infrared focal plane imaging technology has been widely used in target detection and orientation, reducing pixel-pitch or increasing the number of infrared sensor array pixels was unable to meet the practical requirement for the restriction of infrared sensor’s processing technology. This paper proposes a approach that apply multi-element detector array in CS imaging system. The coded measurement technology which uses optical mask and realizes compressed sampling of target scene is described in this paper, on the basis of summarizing CS theory and algorithm. Considering the fact that the reconstruction process of large size image lasts a long time, this paper also presents a high quality image partition CS method, which requires less computing time. The simulation results point the best choice for image partition which requires the least computing time. The validity of this method also been testified using Lena gray image. Finally, according to the inconformity of recovered image via TVQC and TVEQ in pixel lever induced by image stitching, this paper presents a method to decrease the gray differences between recovered imag e blocks. Such method can effectively improve the PSNR value of recovered image, which provides a effective reference in high-resolution infrared CS imaging.

Clinical trials have demonstrated that pediatric acute promyelocytic leukemia (APL) is highly curable. Small-scale studies have reported on the treatment of APL using one or two treatment regimes. Here, we report a multiple center-based study of 119 cases of pediatric APL treated with four regimes based on all-trans-retinoic acid (ATRA). We retrospectively analyzed the clinical characteristics, laboratorial test results and treatment outcome of the pediatric APL patients. Regime 1 used an in-house developed protocol, regime 2 was modified from the PETHEMA LPA99 protocol, regime 3 was modified from the European-APL93 protocol, and regime 4 used a protocol suggested by the British Committee for Standards in Haematology. The overall complete remission rates for the four regimes were 88.9, 87.5, 97.1 and 87.5%, respectively, which exhibited no statistical difference. However, more favorable results were observed for regimes 2 and 3 than regimes 1 and 4, in terms of the estimated 3.5-year disease-free survivals, relapse rates, drug toxicity (including hepatotoxicity, cardiac arrhythmia, and differentiation syndrome) and sepsis. In conclusion, the overall outcomes were more favorable after treatment with regimes 2 and 3 than with regimes 1 and 4, and this may have been due to the specific compositions of regimes 2 and 3.

By using the spatially explicit forest landscape model (LANDIS 6.0 PRO), the percentages of timber-harvesting area in Xiao Xing' anling Mountains under current harvesting scenario and under 11 harvesting scenarios with alternating harvesting intensities (alternating time was 10, 20 and 30 years, respectively) in 2000-2400 under climate change were simulated. Alternating harvesting intensity could increase the harvesting area. Comparing with current harvesting scenario, the simulated scenarios could increase the harvesting area by 3%-5% at short term (10-30 years), 2.5%-7% at medium term (40-60 years), and 3.5%-8% at long term (70-100 years). On the whole, the current total harvesting area was still high. Alternating harvesting-intensity could increase the harvesting area within a definite term, but the effect would be unsustainable. To have a sustainable development of forestry in the study area, it is necessary to reduce the harvesting intensity and change the forest management policy.

For a concentric spherical system composed of two electrodes with electrostatic focusing, the electrostatic potential distribution and the spatial-temporal trajectory of electron motion can be expressed by analytical forms. It is naturally to take such system as an ideal model to investigate the imaging properties, as well as the spatial-temporal aberrations, to analyze its particularity and to find the clue of universalities and regularities. Research on this problem can afford academic foundation not only in studying the static imaging for the night vision tube, but also in studying the dynamic imaging for high speed image converter tube. In the present paper, based on the practical electron ray equation and electron motion equation for a bi-electrode concentric spherical system with electrostatic focusing, the spatial-temporal trajectory of moving electron emitted from the photocathode is solved, the exact and approximate formulae for image position and arriving time, have been deduced. From the solution of spatial-temporal trajectory the electron optical spatial and temporal properties of this system are then discussed, the paraxial and geometrical lateral aberrations with different orders, as well as the paraxial and geometrical temporal aberrations with different orders, are defined and deduced, that are classified by the order of (e z /Φ ac ) 1/2 and (e r /Φ ac ) 1/2

The paraxial solutions play an important role in studying electron optical imaging system and its spatial-temporal aberrations, as was discussed in previous paper [1] , but investigation of a bi-electrode concentric spherical system with electrostatic focusing directly from paraxial electron ray equation and paraxial electron motion equation has not been done before. In this paper, we shall use the paraxial equations to study the spatial-temporal trajectories and their aberrations for a bi-electrode concentric spherical system with electrostatic focusing. In the present paper, start from the paraxial ray equation and paraxial motion equation, the paraxial spatial-temporal trajectory of moving electron emitted from the photocathode has been solved for a bi-electrode concentric spherical system with electrostatic focusing. The paraxial static and dynamic electron optics, as well as the paraxial spatial-temporal aberrations in this system are then discussed, the general regularity of imaging in paraxial optical system has been explored. The paraxial spatial aberrations, as well as the paraxial temporal aberrations with different orders, have been defined and deduced, that are classified by the order of ( ɛ z / ϕ ac ) 1/2 and ( ɛ T / ϕ ac ) 1/2 . Thus we get same conclusions about paraxial spatial and temporal aberrations as we have given in the previous paper and it completely shows that the paraxial spatial-temporal aberrations can be investigated directly from the paraxial ray equation and paraxial motion equation.

To evaluate the therapeutic effects of a combined immunotherapy, high-dose immunoglobulin (HDIG) plus cyclosporine A (CsA) plus prednisone (P), in children with aplastic anemia (AA) and to explore the association of peripheral blood lymphocyte subsets, peripheral blood cells and marrow CD34+ cells with therapeutic effects in AA.The clinical data of 46 children with AA and who received the combined immunotherapy of HDIG + CsA + P were retrospectively studied.Of the 46 children with AA, 31 (67.4%) were responded to the combined immunotherapy. The binary logistic regression analysis showed low absolute neutrophil count (B=4.703, p0.05), low percentage of peripheral blood CD4+ cells (B=0.142, p0.05) and low ratio of peripheral blood CD4+/CD8+ (B=2.945, p0.05)were associated with poor therapeutic effects. The ratio of CD34+/karyocytes of bone marrow in children with AA was lower than that in normal individuals, but it was not significantly related to the therapeutic effect.The combined immunotherapy (HDIG+CsA+P) was effective in children with AA. The absolute neutrophilcount, the percentage of peripheral blood CD4+ and the ratio of peripheral blood CD4+/CD8+ were important prognostic factors in AA.

To investigate the effects of intrathecal ouabain and tizanidine injection for treatment of neuropathic pain in rats.Male SD rats weighing 250-300 g were randomly divided into 5 groups (n = 6), namely the control group, ouabain group, tizanidine group, combined ouabain and tizanidine injection group, and the antagonist group. Intrathecal catheter was implanted 7 days before spinal nerve ligation to establish the neuropathic pain model. Mechanical withdrawal threshold (MWT) before and after intrathecal administration of the agents was recorded in the rats. Isobolographic analysis was performed to evaluate the interactions between the agents.Intrathecal injection of ouabain (0.25-5 microg) or tizanidine (0.5-5 microg) alone produced dose-dependent analgesic effect against the neuropathic pain (P0.05). Isobolographic analysis revealed a synergistic interaction between ouabain and tizanidine. Intrathecal pretreatment with atropine (5 microg) or yohimbine (20 microg) antagonized the effects of ouabain and tizanidine administered alone or in combination (P0.05).Intathecal injection of ouabain or tizanidine produces dose-dependent analgesic effects against neuropathic pain, and their synergistic effect after combined injection probably involves the cholinergic transmission and alpha2 receptor.

The ALL-XH-99 protocol for the treatment of childhood acute lymphoblastic leukemia (ALL) has been performed in the Union Hospital for 10 years. This study aimed to evaluate the therapeutic effectiveness of the protocol for childhood ALL and to investigate the prognostic factors for childhood ALL.This is a retrospective study. The eligible patients were treated with the ALL-XH-99 protocol. However a minor modification based on the ALL-XH-99 protocol was performed in this study, i.e., the high-risk patients as the low- and moderate-risk patients were not administered with cranial irradiation. Event-free survival (EFS) was evaluated using the Kaplan-Meier method and the differences of the EFS among groups were compared with the log-rank test. Prognostic factors for childhood ALL were investigated by the stepwise Cox proportional hazard model.One hundred fifteen patients were eligible for the ALL-XH-99 protocol clinical study. The 115 patients consisted of 62 low-risk, 12 moderate-risk and 41 high-risk patients. The overall EFS at 5 years in the 115 patients was 69.0 +/- 5.0%. The 5-year-EFS in the low-risk, moderate-risk and high-risk patients was 82.0 +/- 6.0%, 77.0 +/- 15.0% and 43.0 +/- 11.0%, respectively (P0.01). Relapse occurred in 16 patients (13.9%) in a median time of 17 months. Without administering cranial irradiation to all of the patients, the incidence of CNS leukemia relapse (2/115, 1.7%) was not higher than that previously reported. Multivariate analysis showed that the risk degree of leukemia, the presence of t (9; 22)/bcr/abl fusion gene and leukocyte count were independent adverse prognostic factors for ALL and their hazard ratio was 1.867, 3.397 and 2.236 respectively.The therapeutic effectiveness of the ALL-XH-99 protocol for childhood ALL is satisfactory, with an EFS rate comparable to that of the developed countries. t (9; 22)/bcr/abl is the most important adverse independent prognostic factor for childhood ALL. Cranial irradiation may be eliminated to reduce late adverse effects in all of ALL patients.

To assess the effectiveness and safety of acupuncture and moxibustion for treatment of ulcerative colitis.Randomized controlled trials or clinical controlled trials of acupuncture and moxibustion for interfere of ulcerative colitis in recent 10 years were reviewed and Meta-analysis was made for the literature results.Altogether 11 papers of clinical study were enrolled. Heterogeneous tests were conducted for the results of the 11 studies, as a result, chi2 = 8.55, P = 0.67. The fixed effect model was used for statistical analysis, after combination OR = 3.82, confidence interval of 95% was 2.65-5.52. The rhombus was located at the right side of the medium line. After Z test, Z = 7.14, P0.01, the therapeutic effect and the cured rate in the treatment group were significantly higher than those of the control group.The therapeutic effect of acupuncture and moxibustion on ulcerative colitis is superior to that of western medicine with safety and less adverse reactions.