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The VEGF receptor is mock-coupled with a known active compound and the active groups of the inhibitor which can bind to VEGF were analyzed. Using asiatic acid as a lead compound, 10 novel skeleton candidate compounds were designed through introduction of the active groups onto the special location and synthesized simultaneously. Furthermore, the structure of these compounds was determined by

The heat transfer and pressure drop characteristics of the mixture are the design basis of evaporators and optimization of Organic Rankine Cycle (ORC). As a promising mixture for ORC systems, R245fa/R141b with different mass fractions (1/0, 0.75/0.25, 0.5/0.5, 0.25/0.75 and 0/1) were selected as the working fluid. The heat transfer coefficient (HTC) and frictional pressure drop in a horizontal microfin tube with 8.6 mm equivalent diameter were measured. During the experimental tests, the saturation pressure is vary from 0.15 to 0.25 MPa (saturation temperature from 25.3 to 60.5 ℃), mass fluxes from 203.19 to 306.64 kg•m-2 s-1, and heat fluxes from 7 to 15 kW m-2. Additionally, based on the empirical correlations in the literature, we developed a correlation with higher prediction accuracy. The results indicate that the HTC and pressure drop of R245fa/R141b increase with mass flux, while decrease with saturation pressure. The increase of the mass fraction of R245fa results in an increase of the HTC and friction pressure drop for R245fa/R141b. The correlation of Cavallini–Kattan are consistent with the experimental data of heat transfer with mean absolute deviations of 16.68%. When mass flux is less than 200 kg m-2 s-1 and vapor quality is less than 0.2, modified Dittus–Boelter correlation can predict 87.3% data points in the error range of ±30% with a MAD of 6.75%. As to frictional pressure drop, the deviations of ±10.34% experimental data are obtained by the modified Filho correlation.

An experimental investigation on the boiling heat transfer and frictional pressure drop of R245fa in a 7 mm horizontal micro-fin tube was performed. The results show that in terms of flow boiling heat transfer characteristics, boiling heat transfer coefficient (HTC) increases with mass velocity of R245fa, while it decreases with the increment of saturation temperature and heat flux. With the increase of vapor quality, HTC has a maximum and the corresponding vapor quality is about 0.4, which varies with the operating conditions. When vapor quality is larger than the transition point, HTC can be promoted more remarkably at higher mass velocity or lower saturation temperature. Among the four selected correlations, KANDLIKAR correlation matches with 91.6% of experimental data within the deviation range of ±25%, and the absolute mean deviation is 11.2%. Also, in terms of frictional pressure drop characteristics of flow boiling, the results of this study show that frictional pressure drop increases with mass velocity and heat flux of R245fa, while it decreases with the increment of saturation temperature. MULLER-STEINHAGEN-HECK correlation shows the best prediction accuracy for frictional pressure drop among the four widely used correlations. It covers 84.1% of experimental data within the deviation range of ±20%, and the absolute mean deviation is 10.1%.

Although cervical cancer is the most common gynecological cancer, it is rare in pregnant women. Treatment of locally invasive cervical cancer during pregnancy is often complex and challenging. Neoadjuvant chemotherapy (NACT) is a possible treatment option. Here we report two cases of cervical cancer diagnosed during pregnancy, one of which was sensitive to NACT, while the other was not sensitive to chemotherapy but showed a good response to concurrent chemoradiotherapy (CCRT) and intra-arterial chemotherapy. Both of these cases have not revealed any signs of tumor recurrence and their children are growing healthily.

Vascular mild cognitive impairment (VaMCI) represents the early stage of symptoms of vascular cognitive impairment (VCI). There are many intervention factors in this period. If the active treatment can delay the further development of the disease and even reduce the risk of transforming into vascular dementia (VaD). As a widely used imaging method, multimode magnetic resonance imaging can evaluate the brain structure and function of patients with VaMCI noninvasively and explore the relationship between brain structure, function and cognitive function change. It is beneficial to provide an idea for early diagnosis of VaMCI and to further understand the neuropathologic mechanism of its occurrence, which has broad application prospects.In this paper, the research status and new methods of VaMCI are reviewed by using multi-mode magnetic resonance imaging in recent years.

In order to improve the overall performance of organic Rankine cycle (ORC) systems, a novel evaluation method using the combination of grey relational method and entropy theory was presented. Based on a comprehensive evaluation indicator, which is constituted by 4 indicators in terms of thermodynamics, economy and environment, system performance was evaluated under different turbine inlet pressure and pinch point temperature difference. Besides, a performance comparison of six different working fluids was conducted. The results show that ORC system can achieve a better comprehensive performance at lower pinch point temperature difference in the evaporator. And there is an optimal turbine inlet pressure, which is significantly influenced by the inlet temperature of exhaust gas, to minimize the value of comprehensive evaluation index (CEI). As a low global warming potential (GWP) working fluid, butane produces the best comprehensive performance for exhaust temperature range of 443–483 K, and R1233zd(E) gives the highest value of CEI in exhaust temperature range of 483–513 K. Additionally, R1234yf is recommended for exhaust temperature lower than 443 K.

We used microfluidic technology for preparing gas-liquid Janus emulsions; we firstly proposed a one-step preparation method of micro-grippers and then characterized the function of oriented and precise delivery behavior. Because of the enrichment of Fe3O4 nanoparticles, the micro-gripper can reach a speed of 1.5 mm s-1 driven by a magnetic field. The micro-gripper's body is made of a poly(N-isopropylacrylamide) hydrogel, a reversible temperature-responsive polymer. The thermo-sensitivity of hydrogels offers the function of grasping, to closely integrate with the target carried, ensuring the stability of the carrying process. The reversible variation of the hydrogel allows the micro-gripper to be reusable and have a long shelf life.

Ethnopharmacological relevance Ginsenoside Rg1 (Rg1), one of the major bioactive ingredients of Panax ginseng C. A. Mey, has neuroprotective effects in animal models of depression, but the mechanism underlying these effects is still largely unknown Aim of the study Gap junction intercellular communication (GJIC) dysfunction is a potentially novel pathogenic mechanism for depression. Thus, we investigated that whether antidepressant-like effects of Rg1 were related to GJIC. Materials and methods Primary rat prefrontal cortical and hippocampal astrocytes cultures were treated with 50 μM CORT for 24 h to induce gap junction damage. Rg1 (0.1, 1, or 10 μM) or fluoxetine (1 μM) was added 1 h prior to CORT treatment. A scrape loading and dye transfer assay was performed to identify the functional capacity of gap junctions. Western blot was used to detect the expression and phosphorylation of connexin43 (Cx43), the major component of gap junctions. Results Treatment of primary astrocytes with CORT for 24 h inhibited GJIC, decreased total Cx43 expression, and increased the phosphorylation of Cx43 at serine368 in a dose-dependent manner. Pre-treatment with 1 μM and 10 μM Rg1 significantly improved GJIC in CORT-treated astrocytes from the prefrontal cortex and hippocampus, respectively, and this was accompanied by upregulation of Cx43 expression and downregulation of Cx43 phosphorylation. Conclusion These findings provide the first evidence indicating that Rg1 can alleviate CORT-induced gap junction dysfunction, which may have clinical significance in the treatment of depression.

In situ sediments were collected at different sites of the Danjiangkou Reservoir using a columnar sediment sampler, and the release rate of N and P at the sediment-water interface was determined through static incubation experiments and the diffusion model of interstitial water molecules. The results showed that there was a significant difference in the release rate for N and P from sediments collected at five sampling sites. The release rates of NH

Transient Receptor Potential Melastatin 4 (TRPM4) is a nonselective channel conducting monovalent ions and indirectly regulates intracellular Ca(2+). Aberrant expression has been reported in a number of cancers. However, the biological function of TRPM4 in endometrial carcinoma (EC) is still unknown. We find that decreased TRPM4 expression is significantly correlated with a poor prognosis, overall survival (OS, P

A novel power and cooling system combined system which coupled organic Rankine cycle (ORC) with vapor compression refrigeration cycle (VCRC) was proposed. R245fa and butane were selected as the working fluid for the power and refrigeration cycle, respectively. A performance comparison and analysis for the combined system was presented. The results show that dual-pressure ORC-VCRC system can achieve an increase of 7.1% in thermal efficiency and 6.7% in exergy efficiency than that of basic ORC-VCRC. Intermediate pressure is a key parameter to both net power and exergy efficiency of dual-pressure ORC-VCRC system. Combined system can produce maximum net power and exergy efficiency at 0.85 MPa for intermediate pressure and 2.4 MPa for high pressure, respectively. However, superheated temperature at expander inlet has little impact on the two indicators. It can achieve higher overall COP, net power and exergy efficiency at smaller difference between condensation temperature and evaporation temperature of VCRC.

The CO

Knowledge of the effect of different vegetation restoration measures on soil organic carbon (SOC) and total soil nitrogen (TN) is of importance to better understand carbon and nitrogen cycling in terrestrial ecosystems and deal with the global greenhouse effect. The differences in SOC and TN content in 0-100 cm soil profile between different restoration measures and their impact factors were investigated in the Loess Plateau. The results showed that artificial vegetation restoration led to a significant increase in both SOC and TN content in the 0-100 cm soil profile compared with that of cropland. The highest increase in average SOC content was observed in the artificial woodland(1.43 times that of the cropland), followed by the artificial shrubland (1.36 times) and artificial grassland (1.21 times); whereas the highest increase in average TN content was observed in the artificial woodland (1.30 times that of the cropland), followed by the artificial grassland (1.21 times) and artificial shrubland (1.13 times). Compared with the cropland, there was a significant difference in SOC and TN content and fine root density up to a maximum depth of100 cm in the artificial woodland and shrubland, but about 60 cm in the artificial grassland. The fine root density, soil C:N ratio and aboveground litter production of artificial woodland, shrubland and grassland were significantly higher than that of cropland, and fine root density was significantly linearly correlated with SOC and TN (P0.01). The quantity and quality of fine root and litter biomass might be the dominant factors contributing to the observed difference in SOC and TN contents between the different artificial vegetation types in the Loess Plateau.研究植被恢复对土壤碳氮动态的影响,对了解陆地生态系统碳氮循环,应对全球温室效应具有重要意义.本研究以黄土丘陵区不同人工恢复植被为对象,以农田为参照,分析了不同人工植被恢复方式对0～100 cm剖面土壤有机碳(SOC)和全氮(TN)含量影响的差异及其影响因素.结果表明: 退耕还林还草显著提高了土壤的SOC和TN含量.退耕后,SOC和TN含量均较农田明显提高.0～100 cm土层SOC平均含量人工乔木林为农田的1.43倍,增幅最大;其次是人工灌木,为1.36倍;最后是人工草地,为1.21倍.0～100 cm土层TN平均含量人工乔木林增幅最大,是农田的1.30倍;其次是人工草地,为1.21倍;而人工灌木增幅最小,为1.13倍.与农田相比,人工恢复植被类型间SOC和TN含量及细根密度的差异在土壤剖面深度上表现出不同,人工乔木和灌木最明显,影响深度＞100 cm;草地最小,仅为60 cm.恢复植被的细根密度、C∶N和凋落物量显著高于农作物,细根密度与SOC、TN呈显著线性相关(

Three-dimensional model of chemical vapor deposition reaction in polysilicon reduction furnace was established by considering mass, momentum and energy transfer simultaneously. Then, CFD software was used to simulate the flow, heat transfer and chemical reaction process in reduction furnace and to analyze the change law of deposition characteristic along with the H2 mole fraction, silicon rod height and silicon rod diameter. The results show that with the increase of H2 mole fraction, silicon growth rate increases firstly and then decreases. On the contrary, SiHCl3 conversion rate and unit energy consumption decrease firstly and then increase. Silicon production rate increases constantly. The optimal H2 mole fraction is 0.8-0.85. With the growth of silicon rod height, SiHCl3 conversion rate, silicon production rate and silicon growth rate increase, while unit energy consumption decreases. In terms of chemical reaction, the higher the silicon rod is, the better the performance is. In the view of the top-heavy situation, the actual silicon rod height is limited to be below 3 m. With the increase of silicon rod diameter, silicon growth rate decreases firstly and then increases. Besides, SiHCl3 conversion rate and silicon production rate increase, while unit energy consumption first decreases sharply, then becomes steady. In practice, the bigger silicon rod diameter is more suitable. The optimal silicon rod diameter must be over 120 mm.

The present study was performed to investigate the therapeutic performance of polymer-lipid hybrid nanoparticles towards the delivery of lapatinib (LPT) in breast cancers. We have successfully developed the lapatinib-loaded polymer-lipid hybrid nanosystem and showed its therapeutic potential in in vitro and in vivo models of breast cancer. The nanoformulations consisted of a polymeric core (poly[lactide-co-glycolide]-D-a-tocopheryl polyethylene glycol 1000 succinate [PLGA–TPGS]), which was then enveloped by a PEGylated lipid layer (DSPE-PEG) (PLPT) to maintain the structural integrity. The PLPT formulation controlled the drug release in pH 7.4 conditions and accelerated the release at pH 5.5 conditions. The PLPT showed a remarkable cellular internalization and efficiently killed the MCF-7 cancer cells in a time- and concentration-dependent manner. Moreover, LPT-loaded nanoparticles effectively induced apoptosis of cancer cells than compared to free LPT. Pharmacokinetic data suggested that nanoparticles could significantly enhance the blood circulation time of LPT by reducing the uptake by a reticuloendothelial system (RES). The prolonged blood circulation of PLPT could allow the preferential accumulation of drug in the tumor tissues. Importantly, PLPT significantly reduced the tumor burden of cancerous mice and effectively controlled the tumor cell proliferation. TUNEL assay further showed a greater apoptosis of tumor tissues in the PLPT treated mice group. Our results suggest that the use of a hybrid system may allow a decrease in the dosage regimen without the loss of therapeutic effect. Overall, lapatinib-loaded hybrid nanoparticles hold great potential for achieving an optimal therapeutic effect in breast cancer treatment. The present anticancer drug delivery system could be potentially applied for the treatment of other cancers.

Due to the impact of multi-period structure in Zhujiahe coal,Mine structure complicated and Has caused a certain impact on coal mine safety production. article Randomly selected 30 from 170 borehole date in coal mine.This paper analyzes 1, 2, 3 times of trend surface on No. 5-2 coal seam floor elevation.exploring the trend surface analysis method research in mine geological structure regular.

The aim of the present study was to investigate the tumor targeting potential of a mannose-conjugated pH-sensitive nanosystem for the effective delivery of gefitinib (Gnb) to lung cancers. We have successfully demonstrated the potential of mannose-tagged nanomicelles as an efficient vector to transport the anticancer drug. The micelles had nanosized particles with excellent dispersity index. The nanomicelles exhibited a pH-responsive nature with enhanced drug release in acidic pH conditions. Fluorescence and flow cytometer analysis showed a superior cellular uptake for the mannose-tagged nanomicelles. Confocal laser scanning microscopy study revealed a receptor-mediated cellular internalization process. The M-NP-Gnb showed a superior anticancer effect in A549 cancer cells and remarkably inhibited cancer cell proliferation. Furthermore, M-NP-Gnb significantly increased the proportion of cells in the apoptosis and necrosis region. Importantly, the half-life of Gnb encapsulated in nanomicelles increased by about 5-fold compared to that of free Gnb. The augmented half-life clearly indicates the maximum residence time of the drug in the systemic circulation. Consistently, M-NP-Gnb showed a 7-fold higher accumulation of drug in tumor tissues compared to that of free Gnb. Overall, PLGA/His-based nanomicelles could be a promising delivery system to increase the therapeutic efficiency of Gnb in lung cancers.

Dynamic performance is important to the controlling and monitoring of the organic Rankine cycle(ORC) system so to avoid the occurrence of unwanted conditions. A small scale waste heat recovery system with organic Rankine cycle was constructed and the dynamic behavior was presented. In the dynamic test, the pump was stopped and then started. In addition, there was a step change of the flue gas volume flow rate and the converter frequency of multistage pump, respectively. The results indicate that the working fluid flow rate has the shortest response time, followed by the expander inlet pressure and the expander inlet temperature. The operation frequency of pump is a key parameter for the ORC system. Due to a step change of pump frequency (39.49–35.24 Hz), the expander efficiency and thermal efficiency drop by 16% and 21% within 2 min, respectively. Besides, the saturated mixture can lead to an increase of the expander rotation speed.

The energy consumption of several central air conditioning systems in summer was researched by the method of exergy analysis. Combined with actual example,the exergy loss of all the equipments and the exergy efficiency of three systems were calculated. The results show that the exergy efficiency of three systems is very low. Relatively speaking, the exergy efficiency of primary return air conditioning system with supplying air in dew point is highest. The equipment of highest exergy loss is air-conditioned room, while the exergy loss of surface air cooler is smallest. Based on this, several improvement measures were proposed to reduce exergy loss and improve exergy efficiency.

The article summarizes the regularity of faults structure by statistics of Geological Exploration and many production geological data , analysis of the characteristics of the fault structure in No.5 coal . The results indicate that there are 6 large and medium-sized faults in No.5 coal and the faults are mainly NEE and NE trending. The small faults are more development in coal area , and the faults are messy but mainly EW trending secondly NW . The fault structure caused certain negative influence to safety production .

Turbine and the working fluid are two key factors that affect the performance of an organic Rankine cycle (ORC). A one-dimensional design for an ORC radial inflow turbine is conducted in this paper. An entire three-dimensional model of the designed turbine including the nozzle, rotor, and volute, is developed and its performance is predicted by Computational Fluid Dynamics. Peng-Robinson equation is employed to predict the properties of working fluids. The simulation shows that the numerical results agree well with the designed value. Additionally, the performance comparison between R245fa and R245fa/R134a is carried out. It is concluded that when R245fa and R134a are mixed with a ratio of 7:3, the power output and isentropic efficiency of the turbine are 9.3% and 2.5% higher than pure R245fa, respectively. What’s more, the off-design performance of the radial turbine using R245fa/R134a is numerically investigated. The results show that there is little change for isentropic efficiency of the radial inflow turbine when the rotational speed varies from 95% rpm to 115% rpm. However, the pressure ratio has a great impact on turbine efficiency. The performance prediction method based on preliminary design and numerical simulation can be applied to the future optimal design of a radial-inflow turbine using zeotropic mixture fluids.

Explains the principle and reaction mechanism of desulfurization catalyst. Introduces the test applying desulfurization catalyst in WFGD (wet flue gas desulfurization) system of 320MW generating units operating in thermal power plant. Desulfurization efficiency is improved significantly by using desulfurization catalyst of 100 μ g/g to 600 μ g/g concentrations. To save electricity, stopping the top level slurry pump is the priority.

An experimental system for heat recovery from low-temperature flue gas based on Organic Rankine Cycle (ORC) was constructed. In the system, R123 was selected as working fluid, a scroll expander was used to produce work, and fin tubes heat exchanger was designed as evaporator. Low-temperature flue gas produced by an liquefied petroleum gas (LPG) stove was used as the heat source to simulate industrial flue gas, and its temperature can be controlled in the range of 90–220 °C. Relationships between output performance of the system and the evaporating pressure, temperature of the heat source as well as the superheat degree of the working fluid were investigated. The results show that the cycle efficiency, the output power of the expander and its exergetic efficiency increase whilst the heat recovery efficiency decreases with the increment of the evaporating pressure at a certain temperature of the heat source. The influence of the superheat degree of the working fluid on the system output parameters is slight. Under the present experimental conditions, the maximum output power of the expander is 645 W, and the cycle efficiency and the heat recovery efficiency are 8.5% and 22%, respectively.

Ginsenoside Rg1 (Rg1) exhibits antidepressant-like activity by increasing neurogenesis and dendritic spine density without discernible side effects. However, the molecular mechanisms underlying Rg1 antidepressant activity remain poorly understood. As the dysfunction of gap junctions between astrocytes in the prefrontal cortex (PFC) is implicated in major depression disorder, the aim of this study was to investigate the effects of Rg1 on astrocyte gap junctions in the PFC. Rats exposed to chronic unpredictable stress (CUS) were administered Rg1 (5, 10, and 20mg/kg) for 28days and analyzed for depressive symptoms using the sucrose preference and forced swimming tests. Functional and morphological changes of gap junction channels in the PFC were evaluated using dye transfer and electron microscopy, respectively. The expression of connexin 43 (Cx43) was analyzed by western blotting. Rg1 markedly alleviated depression-like behavior in rats. Long-term Rg1 treatment of CUS-exposed rats also significantly prevented the decrease in dye diffusion and improved the ultrastructure of astrocyte gap junctions in the PFC, indicating beneficial effects on the functional activity of gap junction channels in the brain. In addition, Rg1 upregulated Cx43 expression in the PFC reduced by CUS exposure, which significantly correlated with its antidepressant-like effects. The results demonstrate that Rg1-induced antidepressant effects are might be mediated, in part, by protecting astrocyte gap junctions within the prefrontal cortex.

Based on the comprehension of the concepts about the post-evaluation of the old industrial buildings group construction progress, this paper proposes the evaluation content and the improved LFA method for the construction progress post- evaluation of the reusing project of the old industrial buildings group Furthermore, this paper conducts the progress post-evaluation on the specific projects and analyses the reasons of these differences. Some suggestions and countermeasures are also put forth to improve the old industry buildings group reusing progress.

At present, Nansi Lake restoration is maily focused on reducing extraneous pollution, however, it is unclear about the endogenous pollution. In this study, twelve intact sediment cores were collected from four sub-lakes (Nanyang Lake, Dushan Lake, Zhaoyang Lake and Weishan Lake) in Nansi Lake, and the fluxes of NH4+-N, PO43--P, NO3--N, TN and TP at sediment-water interface were calculated based on static incubation of sediment cores with a laboratory-scale benthic chamber. The incubation results showed fluxes of PO43--P, NO3--N, TN and TP in Nanyang Lake were the highest and as follows: 2.73, 7.55, 44.43 and 3.06 mg/m2.d, respectivly, and the flux of NH4+-N in Nanyang Lake, Dushan Lake and Zhaoyang Lake had little difference ranged from 8.99 to 10.19 mg/m2.d. This study indicated that during the sampling period sediment acted as a source of nitrogen as well as phosphorus to the overlying water body in Nansi Lake.

Taking the ratio of heat transfer area to net power and heat recovery efficiency into account, a multi-objective mathematical model was developed for organic Rankine cycle (ORC). Working fluids considered were R123, R134a, R141b, R227ea and R245fa. Under the given conditions, the parameters including evaporating and condensing pressures, working fluid and cooling water velocities were optimized by simulated annealing algorithm. The results show that the optimal evaporating pressure increases with the heat source temperature increasing. Compared with other working fluids, R123 is the best choice for the temperature range of 100–180 °C and R141b shows better performance when the temperature is higher than 180 °C. Economic characteristic of system decreases rapidly with the decrease of heat source temperature. ORC system is uneconomical for the heat source temperature lower than 100 °C.

Objectives: The use of gasless laparoscopy with an abdominal wall-lifting device for benign gynecological diseases was compared to conventional laparoscopy with CO2 pneumoperitoneum. Methods: From February 2007 to July 2007, 76 women with uterine and/or adnexal benign diseases and candidates for laparoscopic surgery were recruited in this study. Thirty-two women underwent gasless laparoscopic surgery and 44 women underwent pneumoperitoneum laparoscopic surgery. Results: Diverse pathologies, including adnexal cyst, uterine myoma and ectopic pregnancy, were treated successfully with gasless laparoscopic surgery. Compared with the patients in the pneumoperitoneum group, the similar hospital stay (P=0.353) and intraoperative blood loss (P=0.157) were observed. However, the mean operative time in the gasless group was significantly longer than that in the pneumoperitoneum group (P=0.003). No severe intraoperative or postoperative complications were found in either group, except for one case of laparotomic conversion in the pneumoperitoneum group due to dense pelvic adhesions. The total hospital charges were significantly less in the gasless group than in the pneumoperitoneum group (P=0.001). In 38 cases of ovarian cyst resection, the mean operative time in the gasless group remained longer than that in the pneumoperitoneum group (P=0.017). The total hospital charges were also significantly less in the gasless group than in the pneumoperitoneum group (P

Orchard and black locust are two typical plants for comprehensive control in the small watershed of land uses in Loess area. The analysis of soil carbon sequestration function changes of growing two plants is important to gain a deep understanding of soil carbon cycle process and its influencing factors of terrestrial ecosystems under the condition of small watershed comprehensive control. The experiment was conducted in the Changwu State Key Agro-Ecological Station, Shanxi, China. SOC, TN, fine root biomass and litter amount were determined at different age apple orchards and black locusts on the slope land of Wangdonggou watershed to study the variation characteristics of soil organic carbon and its influencing factors under two measurements. The results showed that: (1) SOC and TN contents in apple orchards significantly decreased with the increased age, whereas those in black locust showed an increased tendency with the age increased. Compared with the adjacent cropland,the SOC and TN contents in year 3, year 8, year 12 and year 18 apple orchards were decreased 3. 26%, 10. 54%, 18. 08%, 22. 55% and - 8. 08%, - 0. 48%, 4. 97%, 16. 91%, respectively. However,SOC and TN contents increased 5. 31%, 32. 36%, 44. 13% and 2. 49%, 15. 75%, 24. 22%, in year 12, year 18 and year 25 black locusts, respectively. (2) The fine root biomass in year 3, year 8, year 12, and year 18 apple orchards were about 25. 97% 66. 23%, 85. 71% and 96. 10% of the adjacent cropland, respectively; and the litter amounts were all 0 g . m-2. However, compared with adjacent cropland, The fine root biomass in year 12, year 18 and year 25 black locusts were increased 23. 53%, 79. 41%, 157. 35%, respectively; and the litter input rates were 194, 298 , 433 g . (m2 . a) -1, respectively. (3) The difference of organic matter input was the major factor which drove the variability of soil carbon sequestration function of apple orchard and black locust ecosystems.

Understanding the effects of nitrogen fertilization on soil respiration rate and its temperature sensitivity (Q10) is of critical importance to predict the variability of soil respiration in cropland. A field experiment was established in a rain-fed spring maize cropland (Zea mays L. ) in the State Key Agro-Ecological Experimental Station in the Loess Plateau in Changwu County, Shaanxi Province, China. The experiment comprised of two treatments: no N-fertilizer application ( CK) and N-fertilizer application with 160 kg N · hm(-2) (N). Soil respiration rate, soil temperature, soil moisture, yields, aboveground biomass and root biomass were measured in two continuous spring maize growing seasons from April 2013 to September 2014. The cumulative soil CO2 emissions were increased by 35% in 2013 and 54% in 2014 in N treatment as compared to CK treatment. Though nitrogen fertilization significantly increased the cumulative soil CO2 emissions (P0.05), it did decrease evidently the temperature sensitivity of soil respiration (P0.05) . The Q10 values in N treatment were decreased by 27% and 17% compared with CK treatment in 2013 and 2014, respectively. Nitrogen fertilization significantly increased the maize yields, aboveground biomass and root biomass (P0.05). Root biomasses in N treatment were 32% and 123% greater than those in CK treatment of 2013 and 2014, respectively. Nitrogen fertilization had no marked influence on soil temperature or moisture. Root biomass was a critical biotical factor for variation of soil respiration under nitrogen fertilization.

The (anaphylactoid) hypersensibility mechanism of ″imprinting templates″ characteristics of traditional Chinese medicine (TCM) injection supramolecules was clarified to lay the foundation to build safety evaluation method. Based on the previous publication on special impact of Chinese medicine theories on supramolcular chemistry, combined with the natural origination of (anaphylactoid) hypersensitized special rules as well as the sensitization phenomenon of cordate houttuynia injection, the impact of the structure characteristics of ″imprinting templates″ in TCM injection supramolecules on its (anaphylactoid) hypersensibility was clarified. In Chinese medicine injections, the supramolecular structures can independently be generated, showing overall apparent (anaphylactoid) hypersensibility nature, and their structure characteristics were dependent on the strength. In addition, (anaphylactoid) hypersensitive critical supramolecular structure was present. When it was administrated by ″injection″, it's structure was not easy to be destroyed, often showing apparent immunogenicity, whereas if it was administrated by ″oral″, the structure would be destroyed by the gastrointestinal tract, showing weaker or no apparent immunogenicity. Therefore, there are differences in (anaphylactoid) hypersensibility between ″injection″ and ″oral″ administration of TCM. TCM injections would produce the supramolecules between ″molecular society″ by independent reaction of supramolecular ″imprinting template″ (chemical determinants), showing apparent immune process of recognition, copying, and storage. Single molecule is a special example for this. The screening of anaphylactoid (sensitinogen) includes the single ingredients and their forming supramolecules for TCM injection. This is the unique feature for safety evaluation of Chinese medicine injection.

Background Oral squamous cell carcinoma (OSCC) or cancers of oral cavity is one of the most common cancers worldwide with high rate of mortality and morbidity. At present, chemotherapy is one of the most effective treatments; however it often fails to meet the requirements in the clinical therapy. In the present study, we have successfully formulated ligand-decorated cancer-targeted CDDP-loaded PLGA-PEG/NR7 nanoparticles and demonstrated the feasibility of using NR7 peptide for targeted delivery, rapid intracellular uptake, and enhanced cytotoxic effect in receptor-overexpressed OSCC cancer cells. Results Nanosized particles were formed and sustained release patterns were observed for PLGA/NR7 nanoparticles. Significantly higher cellular uptake was observed in HN6 OSCC cancer cells and superior anticancer effects are observed from the optimized targeted nanoparticles. Furthermore, Live/Dead assay showed a higher extent of red fluorescence was observed for the cells exposed with PLGA/NR7 than compared with non-targeted PLGA NP. The presence of the NR7-targeting moiety on the surface of PLGA carriers could allow the specific receptor-mediated internalization, enhanced cellular uptake, and higher cell killing potency. Especially, PLGA/NR7 NP exhibited a superior apoptosis effect in HN6 cancer cells with around ~45 % (early and late apoptotic stage) and ~59 % after 24 and 48 h incubation, respectively. It is apparent that the actively targeted micelles will deliver more anticancer agent to cancer cell than non-targeted one. Conclusion Altogether, our results show the feasibility and promise of a cell-targeted anticancer nanomedicine strategy that can be effective for the treatment of oral squamous cell carcinoma. The present work might be of great importance to the further exploration of the potential application of PLGA/NR7 in the clinically relevant animal models.