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To explore the mechanism of circTRRAP (hsa_circ_0081311) in regulating the progression of colorectal cancer (CRC). Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to detect the expression of circTRRAP. Receiver operating characteristic (ROC) curve for circTRRAP was produced. In vitro and in vivo experiments of circTRRAP were conducted. Target miRNAs of circTRRAP were analyzed bymiRanda software. Analyses based on The Cancer Genome Atlas (TCGA) database were conducted to screen the target miRNAs. Luciferase reporter assay was conducted to analyze the interaction between circTRRAP and hsa-miR-194-3p (miR-194-3p). In vitro reverse experiments were conducted to analyze the effect of circTRRAP/miR-194-3p axis on CRC.The results suggested that circTRRAP was up-regulated in CRC patients’ tissues and plasma, and associated with unfavorable clinicopathologic features. The area under curve (AUC) of 0.741 suggested that circTRRAP could serve as a potential biomarker for CRC. CircTRRAP promoted the malignant phenotypes of CRC in vitro and in vivo. CircTRRAP could bind to miR-194-3p, which was significantly down-regulated in CRC tissues and associated with overall survival according to TCGA database. CircTRRAP had no effect on the expression level of miR-194-3p, and miR-194-3p up-regulation had the similar tumor-suppressing effects as circTRRAP down-regulation. The tumor-promoting effect of circTRRAP up-regulation could be reversed by the up-regulation of miR-194-3p. Conclusively, circTRRAP was up-regulated in CRC, and associated with unfavorable clinicopathologic features. CircTRRAP could serve as a promising biomarker for CRC diagnosis. CircTRRAP promoted the progression of CRC by sponging miR-194-3p and could serve as a potential therapeutic target for CRC treatment.

Studies have demonstrated that circular RNAs (circRNAs) play important roles in various types of cancer; however, the mechanisms of circRNAs located in the nucleus have rarely been explored. Here, we report a novel circular RNA circPLCE1 (hsa_circ_0019230) that facilitates the malignant progression of colorectal cancer (CRC) by repressing serine/arginine‐rich splicing factor 2 (SRSF2)‐dependent phospholipase C epsilon 1 (PLCE1) pre‐RNA splicing. Quantitative real‐time polymerase chain reaction was used to determine the expression of circPLCE1 in CRC tissues and cells. Cell Counting Kit‐8, Transwell and flow cytometric assays were used to assess the role of circPLE1 in CRC cell proliferation, migration and apoptosis, respectively. An animal study was conducted to test the role of circPLCE1 in vivo. Furthermore, catRAPID and RPISeq were used to predict the possible binding proteins of circPLCE1. RNA fractionation and RNA immunoprecipitation assays were used to confirm the RNA‐protein interaction. In this study, we found that circPLCE1 was more significantly down‐regulated in CRC tissues compared with that in adjacent normal tissues. However, circPLCE1 knockdown suppressed CRC cell proliferation, migration and invasion and increased apoptosis. Nude mouse experiments showed that ectopic expression of circPLCE1 dramatically increased tumour growth in vivo. Mechanistically, circPLCE1 directly bound to the SRSF2 protein, repressing SRSF2‐dependent PLCE1 pre‐RNA splicing, resulting in the progression of CRC. Individually mutating the binding sites of circPLCE1 abolished the inhibition of PLCE1 mRNA production. Our study revealed a novel molecular mechanism in the regulation of PLCE1 and suggested a new function of circular RNA.

Background Emerging evidence suggests that circular RNAs (circRNAs) are vital regulators in a range of cancers. "miRNA sponge" is the most reported role played by circRNAs in many tumors. The insulin-like growth factor (IGF) 1 pathway plays a key role in the development and progression of many cancers, including colorectal cancer (CRC). The aim of the study is to establish the potential clinical value and driving molecular mechanisms of circRNAs in CRC. Materials and methods Real-time quantitative RT-PCR (qRT-PCR) was performed to measure the circRUNX1 expression in 52 tissue samples from CRC patients. We verified the tumor promotor role of circRUNX1 in cell-based in vitro and in vivo assays. Human growth factor array was used to identify circRUNX1-regulated signaling pathways. We then used a double luciferase reporter assay and RNA fluorescence in situ hybridization to identify the downstream miR-145-5p of circRUNX1. Furthermore, we performed Western blotting and biological function assays to demonstrate if the circRUNX1/miR-145-5p/IGF1 axis is responsible for the proliferation of CRC cells and promotes CRC development. Results By performing qRT-PCR from CRC tissues and paired adjacent normal mucosa tissues, we identified that circRUNX1 expression was significantly upregulated in CRC tissues and positively related with lymph node metastasis, distant metastasis and advanced tumor-node-metastasis tumor stage in patients. Functionally, circRUNX1 knockdown inhibited cell proliferation and migration and promoted apoptosis, whereas its overexpression exerted opposite effects. In vivo, circRUNX1 promoted tumor growth and metastasis. Mechanically, circRUNX1 shared miRNA response elements with IGF1. circRUNX1 competitively bound to miR-145-5p and prevented miR-145-5p from decreasing the expression of IGF1, which facilitated tumor growth. Conclusion Our studies verified that circRUNX1 functions as a tumor promotor in CRC cells by targeting the miR-145-5p/IGF1 signaling pathway and may have potential use as a prognostic indicator and therapeutic target in CRC patients.

To investigate the differentiation of regulatory T cells (Tregs) induced by methylprednisolone (MP) pulse therapy in patients with Systemic Lupus Erythematosus (SLE).We enrolled 30 patients with SLE and analyzed peripheral blood mononuclear cells (PBMCs) before and after MP pulse therapy. Peripheral Tregs, apoptosis of PBMCs subsets, and TGFβ production by monocytes was quantified by flow cytometry. Proliferation and IFN-γ production of CD4Peripheral Tregs was significantly increased after MP pulse therapy (6.76 ± 1.46% vs. 3.82 ± 1.02%, p 0.01), with an expansion of Nrp1MP pulse therapy induces CD4

Background Emerging studies have revealed that circular RNAs (circRNAs) correlate with diverse diseases including cancers. However, little is known about the functions of circRNAs in colorectal cancer (CRC). In our previous research, downregulation of hsa_circ_0140388 (circHUEW1) has been detected in CRC tissues through high-throughput sequencing. However, the underlying mechanism by which circHUWE1 regulates the proliferation and apoptosis in CRC has not been investigated. Materials and methods The levels of circHUWE1 in 58 pairs of CRC tissues and corresponding adjacent healthy tissues were detected by RT-qPCR. In addition, the effects of circHUWE1 on cell proliferation, apoptosis migration and invasion were evaluated by cell proliferation assays, flow cytometry, and transwell assays in HCT116 and SW480 cell lines respectively. Meanwhile, the dual-luciferase reporter system assay was used to explore the interaction between circHUWE1 and miR-486 (hsa-miR-486-5p). Results In this study, we demonstrate that the expression of circHUEW1 is upregulated in CRC tissues. High expression of circHUEW1 was significantly associated with lymphovascular invasion (P =0.036), lymph node metastasis (P =0.017), distant metastasis (P =0.024), and TNM stage (P =0.009). Moreover, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was 0.732, which indicated that circHUWE1 could serve as a potential biomarker in the detection of CRC. Silencing circHUWE1 significantly inhibited the proliferation, migration and invasion capacity of CRC cells in vitro. Mechanistically, we demonstrated that circHUWE1 could sponge miR-486 and the downregulation of miR-486 could reverse the cancer suppressive effects caused by silencing circHUWE1. Conclusion In this study, our results revealed that circHUWE1 may be a potential therapeutic target and diagnostic biomarker for CRC.

Background: Studies have demonstrated that circular RNAs (circRNAs) play important roles in various types of cancer; however, the mechanisms of circRNAs located in the nucleus have rarely been explored. Here, we reported a novel circular RNA circPLCE1 facilitates the malignant progression of colorectal cancer (CRC) via repression of SRSF2-dependent PLCE1 pre-RNA splicing. Methods: qRT-PCR was used to determine the expression of circPLCE1 in CRC tissues and cells. CCK-8, transwell and flow cytometric assays were used to assess the role of circPLCE1 in CRC cell proliferation, migration and apoptosis, respectively. Animal study was carried to test the role of circPLCE1 in vivo. Further, catRAPID and RPISeq were applied to predict the possible binding protein of circPLCE1. RNA fractionation and RIP assays were used to confirm the RNA-protein interaction. Results: In this study, we found that circPLCE1 was significantly downregulated in CRC tissues compared with adjacent normal tissues. However, circPLCE1 knockdown suppresses CRC cell proliferation, migration, invasion and increased apoptosis. Nude mice experiments showed that ectopic expression of circPLCE1 dramatically increased tumor growth in vivo. Mechanically, circPLCE1 directly binding to SRSF2 protein, repressing SRSF2-dependent PLCE1 pre-RNA splicing, resulting in the progression of CRC. Individually mutating the binding sites of circPLCE1 derepressed the production of PLCE1 mRNA. Conclusions: Our studies revealed a novel molecular mechanism in the regulation of PLCE1 and implicated a new function of circular RNA, supporting the pursuit of circPLCE1 as a potential tool for future CRC treatment.

Background Fibulin-5 has recently been considered as a potential tumor suppressor in human cancers. Several studies have shown that it is down-regulated in a variety of tumor types and inhibits tumor growth and metastasis. In this study, the expression of fibulin-5 in colorectal cancer (CRC) and its clinical significance were assessed. Methods Fibulin-5 expression was detected in 31 samples of surgically resected CRC and paired noncancerous tissues using western blot, qRT-PCR, and immunoblotting. Results In this study, the expression levels of fibulin-5 protein and mRNA were down-regulated in CRC tissues as compared with those in paired noncancerous tissues. Low expression of fibulin-5 was significantly correlated with poor prognostic features including lymph node metastasis and advanced tumor-node-metastasis (TNM) tumor stage. Conclusions Fibulin-5 is down-regulated in CRC and the reduced expression of fibulin-5 was correlated with malignant clinicopathological characteristics.

The system of uranyl nitrate aqueous solution and tributylphosphate (TBP) dissolved in kerosene is widely used all around the world, no matter in nuclear fuel cycle or analysis of impurities in uranium. The extraction process is mature, but the amount of consumption of organic reagent and generation of radioactive waste solution is huge, that is not environment friendly. Microfluidic is very popular nowadays, and one of its many applications is extraction. Here in this paper, we studied the flow situation of water and 20%TBP-kerosene, find the relationship between width ratio and flow rate ratio. And we used chemical modification to get a good separated outlet, and we observed the stability of OTS modified glass, that is stable in our circumstance. Finally we use the modified channels to achieve the extraction process, and find the extraction ratio is increases by contact time increases, and has no relationship of initial concentration.

GEO-groove simulation experiment is applied to imitate the Taiyuan soil subsidence in pipeline base and side by soil loss, which eventually leads the pipeline to be deformed and destroyed. The pre-damage standard is set up though analyzing test statistics and drawing it’s sedimentation and damage curve. The damage standard of sewage line is achieved through setting up a very pilot model to the test and analyzing it’s bearing force characteristics, and according to the gotten sedimentation curve of the test, damage characteristics and mechanism of sewage line is analyzed , thus will give the designing and application of pipeline of testing and theory basement, so as to take measures to protect pipeline in practical situations, establish early warning system to pipeline, and guarantee it’s regular service.

For fabrics, the diversification and superior of the properties and the styles can be achieved by blending fibers. To keep up with the demand of the market, PTT, PLA and Rayon were selected as the materials and 10 kinds of PTT/PLA/Rayon blended fabrics with different fiber content were designed and woven. And then the wearabilities of the fabrics were tested respectively. At last, fuzzy evaluation wad used to discuss the affection of the blended ratio on the fabric’s wearability. The results show that various kinds of wearabilities of the fabric are the best when the blending ratio is PTT/ PLA/Rayon 40/30/30.

For gaining the best position ranges of shear sensor on webs, this paper make a finite-element analysis on force measurement rail's webs of shear rail weigher. And we also use a standard force measurement equipment to test it, the test results showed that the repetitive accuracy of rail weighing system reached national standards 0.2 level, international standards, 0.5 level .

By establishing finite element model of load floor based on the field data, this work evaluated vibration of load floor after isolation measures were taken through effective value of acceleration of vibration. And it demonstrated that the floating floor slab was very useful for vibration isolation for load floor whose buildings have been completed. What’s more, the thesis also provided reference standards for further studies on vibration isolation for load floor caused by large-scale rotating machinery on the floor.