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To explore the expression of CD40/CD40L in multiple myeloma(MM) patients and its influence on prognosis.Thirty patients with MM treated in Cangzhou People's Hospital from May 2016 to June 2017 were selected and divided into MM group, then 30 healthy people with a physical examination in our hospital at the same time were selected as the normal group. The serum CD40/CD40L levels of the patients in the two groups was detected by flow cytometry, and its correlation with the lymphocyte population, pathological grade and prognostic significance of MM patients was anaysis.The expression of CD40 in serum of the patients in MM group was significantly higher than those in normal group (P0.05). The expression of CD40L in serum of the patients in MM group showed no significant difference as compared with those in normal group (P0.05). The levels of CD40 and CD40L in the patients before and after chemotherapy showed no difference(P0.05). The levels of Ts and NK cells in the patients of MM group were lower than those in normal group (P0.05). The proportion of total B lymphocytes, Th and Th/Ts cells between the two groups showed no significant difference (P0.05). The CD40 level was correlated with the serum total B lymphocyte level of the patients in MM group (r=0.877, P=0.005). There was a correlation with CD40L and Th cells in the serum of MM patients (r=-0.783, P=0.035). The expression of serum CD40 in the patients at phase III-IV was higher than those of the patients at phase I-II, the levels of serum CD40L in MM patients at different periods showed no significant difference(P0.05). The survival rate of MM patients with high CD40 expression was lower than that of MM patients with low CD40 expression (χThe increasing of CD40 level in MM patients is related to the pathological grade of the patients. Chemotherapy can reduce the level of CD40. The increasing of CD40 is an important factor for the poor prognosis of MM patients. CD40L level is not meaningful for MM treatment and prognosis.CD40/CD40L在MM患者中的表达及其对预后的意义.探讨CD40/CD40L在多发性骨髓瘤（MM）患者中的表达及其对预后的影响.选取2016年5月-2017年6月于沧州市人民医院接受治疗的MM患者30例，作为MM组，同期选取在本院体检的健康人30例为正常组，采用流式细胞术检测两组人员血清中化疗前后CD40/CD40L的水平，分析其与MM患者淋巴细胞群、病理分级及预后的相关性.MM组患者血清中CD40表达水平显著高于正常组（P0.05）；MM组患者血清中CD40L表达水平与正常组比较无统计学意义（P0.05）；MM组患者化疗前后的CD40、CD40L水平比较无统计学差异（P0.05）；Ts细胞及NK细胞水平在MM组患者中的表达低于正常组（P0.05），总B淋巴细胞、Th细胞、Th/Ts占比两组比较无统计学意义（P0.05）；CD40水平与MM组患者血清总B淋巴细胞水平存在相关性（r=0.877，P=0.005），CD40L与MM患者血清中Th细胞存在相关性（r=-0.783，P=0.035）；III-IV期MM患者血清CD40表达高于I-II期MM患者，不同时期MM组患者血清CD40L水平比较无统计学意义（P0.05）；CD40高表达MM患者生存率低于CD40低表达的MM患者（χMM血清中CD40水平升高与患者病理分级有关，接受化疗能够降低CD40水平，CD40升高是MM患者预后较差的重要因素，CD40L水平与MM治疗及预后无意义.

Individuals' right to privacy includes control over access to their location information. With the advent of location-based services and personal transport services (such as ridesharing), the risk of location privacy breaches is increased greatly. The potential negative effects of location privacy leakages include spam location-based service flooding, threats to personal safety (such as physical attacks), and intrusion related to access to private places (such as homes and hospitals). Therefore, protecting the privacy of users' real locations is becoming increasingly important. This is often achieved using a pseudo-location near the real location, but existing pseudo-location generators, such as NRand and the uniform random method, suffer from statistical inference, which can infer the obfuscation domain to cover the real location. In this paper, we propose an intelligent pseudo-location recommendation (IPLR) method to reduce the risk of a statistical inference attack. In IPLR, we generate a random substitute of the real location to attract the adversary and thus hide the real location. Then, the pseudo-location is generated in the neighborhood of the random substitute location following a normal distribution; the random substitute location is changed frequently to confuse attackers. In particular, we define three levels of location privacy, ie, address level, street level, and district level, to evaluate the effectiveness of the IPLR method. Our experimental study using simulation data demonstrates that the proposed IPLR method achieves lower risk of location privacy leakage and higher probabilities of safety in all three levels of location privacy than NRand and the random method. It also demonstrates the effectiveness of the proposed IPLR to balance location privacy and service quality.

To mine the medication rules of herbal prescriptions for gout caused by heat-damp accumulation syndrome, and explore the possible mechanism of the core herbs, we collected the relevant literature of gout caused by heat-damp accumulation syndrome in CNKI, medication rules of herbal prescriptions are analyzed by using TCMISS(V2.5) software, and the compatibility of core drugs and new prescriptions were mined.KEGG pathway analysis was performed by BATMAN-TCM, an online analysis tool, and the potential signaling pathways of core drugs compatibility to treat gout caused by heat-damp accumulation syndrome were revealed. The results showed that six core drugs and three new prescriptions were found out of the 136 prescriptions. The core compatibility herbs in clinical treament of gout caused by heat-damp accumulation syndrome, Phellodendri Chinrnsis Cortex, Achyranthis Bidentatae Radix and Achyranthis Bidentatae Radix, which potential signaling pathways were purine metabolic pathway and neura activity ligand receptor interaction signaling pathway. Therefore, for gout caused by heat-damp accumulation syndrome, the mainly used therapies of TCM were clearing heat and drying dampness,inducing diuresis for removing edema, dispeling wind and eliminating dampness. The mechnisms of core compatibility herbs may be achieved through the intervention of purine metabolic pathway and neural activity ligand receptor interaction signaling pathway.

To investigate the effect of Lang-chuang-ding Decoction (, LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells (PBMCs) of females with systemic lupus erythematosus (SLE).PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The mRNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction (PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR.After treated with medicated serum for 48 h, the mRNA expression levels of CD70 in PBMCs of SLE patients were signifificantly higher than those of healthy donors (P0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were signifificantly higher than those treated with fetal bovine serum (P0.01).LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.

To explore the effects of three Chinese herbal antidotes, i.e. Herba Artemisiae annuae (A), Herba Hedyotis diffusae (H) and Rhizoma Cimicifugae (C), all were ingredients of Jiedu Quyu Ziyin Recipe, for adjusting the regulated on activation normal T cell expressed and secreated (RANTES), gene expression in serum and renal tissue of MRL/lpr mice.Fifty-four MRL/lpr mice were randomized into 9 groups, with 6 in each, and intragastrically infused with A, H, C, A+H, H+C, A+C, A+H+C (all in dosage-form of decoction), prednisone suspension and physiological saline, respectively for 12 weeks. RANTES expression in serum and renal tissue of animals were detected with ELISA and RT-PCR at the end of the study.Levels of RANTES expression was significantly reduced in the prednisone treated group after treatment. Excepting no significant change being observed in the groups treated with A and C, the changes in the other groups were all milder than those in the group treated with A+H+C.Chinese herbal antidotes A, H and C in combination can significantly inhibit the RANTES expression in serum and renal tissue of MRL/lpr mice.

To explore the expression of human leucocyte antigen DR (HLA-DR) gene in peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients of various TCM syndrome types in order to seek an objective index for syndrome differentiation of SLE.SLE patients were sorted into various syndrome types by TCM syndrome differentiation, i. e. Pi-Shen yang-deficiency type (A), yin-deficiency with internal heat type (B), qi-yin deficiency type (C) and Gan-Shen yin-deficiency type (D). Their peripheral blood sample was collected for detecting HLA-DR positive lymphocytes and monocytes in PBMC using flow cytometry.Comparisons among patients of various syndrome type showed that the proportion of HLA-DR positive monocytes and lymphocytes in PBMC in patients of type A was the lowest (P0.01); proportion of HLA-DR positive lymphocytes in patients of type B was significantly higher than in those of type C and D (P0.01), and that in type D was higher than in type C (P0.05); total proportion of HLA-DR positive cells in patients of type A was the lowest, that in type B was higher than that in type C and D (P0.01, P0.05), while no significant difference was found between the latter two types (P0.05).HLA-DR could be taken as an objective index for reference in syndrome differentiation of SLE.

We propose an efficient approach to mine frequent Itemsets on data streams. It is a memory efficient and accurate one-pass algorithm that can deal with batch updates. The proposed algorithm performers well by dividing all frequent itemsets into frequent equivalence classes and pruning all redundant itemsets except for those that represent GLB (Greatest Lower Bound) and LUB (Least Upper Bound) of the frequent equivalence classes. The number of GLB and LUB is much less than the number of frequent itemsets. The experimental evaluation on synthetic and real datasets shows that the algorithm is very accurate and requires significantly lower memory than other well-known one-pass algorithms.