14 results on '"Zhi H. Li"'
Search Results
2. Effect of temperature and salinity on mtDNA copy number of the ridgetail white prawn, Palaemon carinicauda Holthuis, 1950 (Decapoda, Palaemonidae)
- Author
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Jin Q. Sun, Bin L. Yan, Zhi H. Li, Pei Zhang, Huang Gao, Wan Y. Xu, Hang K. Ma, and Qing Q. Zhang
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0106 biological sciences ,Mitochondrial DNA ,Decapoda ,010604 marine biology & hydrobiology ,Temperature salinity diagrams ,Zoology ,010501 environmental sciences ,Aquatic Science ,Biology ,Palaemon carinicauda ,biology.organism_classification ,01 natural sciences ,Salinity ,White (mutation) ,Prawn ,Animal Science and Zoology ,Palaemonidae ,0105 earth and related environmental sciences - Abstract
To explore the effect of temperature and salinity on the mitochondrial DNA (mtDNA) copy number of Palaemon carinicauda Holthuis, 1950, 5 temperature groups (10, 15, 20, 25 and 30°C) and 6 salinity groups (10, 15, 20, 25, 30 and 35) were set up, respectively. Subsequently, the numbers of copies of mtDNA of samples from all groups were detected by the TaqMan probe method. The results showed that the mtDNA copy number in the temperature samples was 2388, 2366, 4158, 4805 and 6027 at the above-mentioned temperature values, respectively. Obviously, the number of mtDNA copies in the cell tends to increase as temperatures rise. In addition, the mtDNA copy numbers of the salinity samples was 2609, 2593, 3215, 3478, 2618 and 2709, respectively, at the experimental salinities as listed above. This indicates, that the copy numbers of mtDNA tend to increase at first, and then again to decrease as the salinity values rise and pass beyond a threshold.
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- 2018
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3. Characterization of an MHC gene in Palaemon carinicauda (Holthuis, 1950) (Caridea: Palaemonidae) and its expression profiles at different post-molt time points
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Bei Xue, Zhi H. Li, Lian Zhao, Huan Gao, Pei Zhang, Hang K. Ma, Wan Y. Xu, Bin L. Yan, and Jin Q. Sun
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0301 basic medicine ,biology ,Zoology ,04 agricultural and veterinary sciences ,Anatomy ,Aquatic Science ,Palaemon carinicauda ,biology.organism_classification ,Major histocompatibility complex ,03 medical and health sciences ,Caridea ,030104 developmental biology ,biology.animal ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Gene ,Palaemonidae - Published
- 2017
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4. Molecular characterization and expression of the p38 MAPK gene from the ridgetail white prawn, Palaemon carinicauda Holthuis, 1950 (Decapoda, Palaemonidae)
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Xiao F. Lai, Pei Zhang, Bei Xue, Bin L. Yan, Zhi H. Li, Lian Zhao, Jian H. Chen, and Huan Gao
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0301 basic medicine ,MAPK/ERK pathway ,biology ,Kinase ,p38 mitogen-activated protein kinases ,Anatomy ,Aquatic Science ,biology.organism_classification ,Molecular biology ,03 medical and health sciences ,Open reading frame ,030104 developmental biology ,Complementary DNA ,Animal Science and Zoology ,Hepatopancreas ,Gene ,Moulting - Abstract
The p38 mitogen-activated protein kinases (MAPK) are a kind of Ser/Thr protein kinases that convert extracellular stimuli into a wide range of cellular response, appearing to function not only in stress stimuli but also in development. To explore the function of p38 MAPK in Palaemon carinicauda Holthuis, 1950, we cloned and characterized the full-length cDNA sequence (GenBank accession number KX893515) (designated as Pc-p38). The results showed that the open reading frame (ORF) of Pc-p38 was 1098 bp and it encoded a protein of 365 amino acids. Pc-p38 contained the conserved structures of a Thr-Gly-Tyr (TGY) motif and a substrate-binding site, Ala-Thr-Arg-Trp (ATRW), and was shown to have a close phylogenetic relationship to other p38 MAPKs in crustaceans. The tissue distribution patterns showed that Pc-p38 was widely expressed in all tissues, with highest expression in the hepatopancreas and ovary. Quantitative real-time PCR revealed that Pc-p38 was upregulated during ecdysis, reaching a peak at 5 min post-moult, suggesting that Pc-p38 may be involved in muscle remodeling after moulting. In addition, the expression of Pc-p38 increased following exposure to different concentrations of mercury, in a dose- and time-dependent manner. In conclusion, an Pc-p38 gene was cloned and its role determined at different times post-moult and after stress from different concentrations of mercury, to further reveal the possible functions of p38 MAPK in P. carinicauda.
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- 2017
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5. Contributors
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Marwan Al Heib, Gerrie van Aswegen, Ömer Aydan, Wilson Blake, Ming Cai, Bing-Rui Chen, Dongfang Chen, Linming Dou, Xia-Ting Feng, Guangliang Feng, Victor I. German, Gareth Goldswain, W.L. Gong, Yossef H. Hatzor, M.C. He, Ben-G. He, Peter K. Kaiser, Seweryn Kokot, Rui Kong, Petr Konicek, Juliusz Kuś, Stanisław Lasocki, Zhi-H. Li, Shaojun Li, Zhihua Li, Jian-Po Liu, Caiping Lu, Richard Lynch, Aleksander Mendecki, Pengzhi Pan, Krishna Kanta Panthi, Shili Qiu, Malyala V.M.S. Rao, F.Q. Ren, Vladimir Shumila, Jan Šílený, Gaolei Song, Chinnappa Srinivasan, Guoshao Su, Boris G. Tarasov, Cezar-Ioan Trifu, Zhaofeng Wang, Xin Wang, Ya-Xun Xiao, Hong Xu, Fei Yan, Yang Yu, Zbigniew Zembaty, Xiwei Zhang, Zhouneng Zhao, Hong Zheng, and G.L. Zhu
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- 2018
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6. Case Histories of Rockburst at Coal Mines
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Caiping Lu, Linming Dou, and Zhi-H. Li
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Mining engineering ,business.industry ,0211 other engineering and technologies ,Coal mining ,02 engineering and technology ,010502 geochemistry & geophysics ,business ,01 natural sciences ,Geology ,021102 mining & metallurgy ,0105 earth and related environmental sciences - Published
- 2018
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7. Contents Vol. 7, 2007
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Maxim S. Petrov, Alexander S. Gordetzov, J. A. Murphy, György M. Buzás, Timothy A. Woodward, Werner Van Steenbergen, Ru F. Chen, H. Oettle, Valerio Di Carlo, Zhi H. Li, Osman Yüksel, Quan B. Zhou, H. Riess, Eduardo Moreno-Osset, Marco Del Chiaro, Lorenzo Fantini, Gwen Lomberk, John A. Dumot, Daniel H. Palmer, Maria A. Caligo, Michelle A. Anderson, Fen Y. Yu, Mark J. Truty, Massimo Falconi, Silvano Presciuttini, Gulsen Yilmaz, David N. Criddle, Roberto Salvia, Ömer Başar, Fernando Gómez, Antonio López, Giorgio Casari, Emre Yıldırım, Jia J. Zhou, Maria Schipper, R.K. Vasishta, Martin E. Fernandez-Zapico, Mansour A. Parsi, María Jesús Martínez, R. Sopena, Pankaj Jain, Robert Sutton, J.D. Wig, Daniel D. Billadeau, Surakit Pungpapong, Jie Wang, Raffaele Pezzilli, P. Neuhaus, Alvaro Pascual-Leone, Euan McLaughlin, Hein G. Gooszen, S.K. Arora, Ji S. Chen, Steven D. Freedman, James M. Scheiman, J. Krysa, Karel J. van Erpecum, Mehmet Ibis, L. Bertacca, Laura Seynaeve, Qi B. Tang, Alessandro Zerbi, Raul Urrutia, Franco Mosca, Madhusudhan R. Sanaka, Nora Sartori, Kyung W. Noh, Paolo Pederzoli, Ricardo A. Cruciani, A. Steger, Ole H. Petersen, S. Majumdar, Leticia de la Cueva, Mikhail V. Kukosh, Fatma Meric Yilmaz, Zeynel Abidin Öztürk, Felipe Fregni, Antonio Maria Morselli-Labate, Philip R. Fischer, D.K. Bakshi, Tomás Ripollés, L. Singh, Biancamaria Longoni, Marco Polese, Matthew J. DiMagno, Erik Buskens, Ugo Boggi, Joel H. Rubenstein, Annemarie C. de Vries, Sandeep Nijhawan, Massimo Raimondo, Seyfettin Köklü, Qiu H. Pan, Marc G. Besselink, Michael B. Wallace, Mohammad A. Al-Haddad, and Ben U. Ridwan
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Hepatology ,Traditional medicine ,business.industry ,Endocrinology, Diabetes and Metabolism ,Gastroenterology ,Medicine ,business - Published
- 2007
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8. In vivo Radioiodide Imaging and Treatment of Pancreatic Cancer Xenografts after MUC1 Promoter-Driven Expression of the Human Sodium-Iodide Symporter
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Qiu H. Pan, Zhi H. Li, Fen Y. Yu, Quan B. Zhou, Jie Wang, Ru F. Chen, Qi B. Tang, Jia J. Zhou, and Ji S. Chen
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Sodium-iodide symporter ,Endocrinology, Diabetes and Metabolism ,Molecular Sequence Data ,Fluorescent Antibody Technique ,Mice, Nude ,Iodine Radioisotopes ,Mice ,In vivo ,Cell Line, Tumor ,Pancreatic cancer ,Animals ,Humans ,Medicine ,Promoter Regions, Genetic ,skin and connective tissue diseases ,MUC1 ,Base Sequence ,Symporters ,Hepatology ,business.industry ,Mucin-1 ,Gastroenterology ,Iodides ,medicine.disease ,Xenograft Model Antitumor Assays ,Pancreatic Neoplasms ,Symporter ,Cancer research ,Female ,CA19-9 ,Tumor-associated glycoprotein 72 ,business ,Ovarian cancer ,HeLa Cells - Abstract
Mucin 1 (MUC1) is a transmembrane glycoprotein that is overexpressed in many tumor types, including breast, pancreatic, and ovarian cancer. The aim of this study was to create a construct containing sodium-iodide symporter (NIS) under the control of the 0.8-kb MUC1 promoter to infect pancreatic cancer cells both in vitro and in vivo, to investigate the potential for radioiodide imaging and ablation of this disease.We amplified the 797-bp MUC1 promoter by two-step nested PCR. Subsequently, a replication-deficient adenoviral construct was created containing the MUC1 promoter followed by the human NIS gene. Iodide uptake assays and immunofluorescence were used to confirm NIS expression and function. Pancreatic cancer xenografts in mice were infected with Ad/MUC1/NIS and then imaged and treated using radioiodide.A 23- and 15.5-fold increase in iodide uptake was observed in Ad/MUC1/NIS-infected MUC1-positive Capan-2 and SW1990 cells with no significant increase observed in MUC1-negative Hela cells or in cells infected with the control virus. The in vivo study showed a clear image of Ad/MUC1/NIS-infected tumor xenografts using (125)I. Administration of a therapeutic dose of (131)I resulted in a regression in size to 76 +/- 15% of their original volume, whereas control tumors continued to increase in size to200% of their original volume.These results show that the 0.8-kb MUC1 promoter was successfully used to drive human NIS-targeted expression in pancreatic cancer cells, and Ad/MUC1/NIS-mediated radiotherapy can make pancreatic cancer xenografts in mice shrinking. This could potentially have applications for both imaging and therapy in other MUC1-positive tumors.
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- 2007
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9. Tricistronic viral vectors co-expressing interleukin-12 (1L-12) and CD80 (B7-1) for the immunotherapy of cancer: Preclinical studies in myeloma
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Zhi H. Li, Robert G. Hawley, Xiao-Yan Wen, A. Keith Stewart, Frank L. Graham, Mary Hitt, Saul Mandelbaum, and Teresa S. Hawley
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Cytotoxicity, Immunologic ,Cancer Research ,DNA, Complementary ,T-Lymphocytes ,medicine.medical_treatment ,Genetic Vectors ,Lymphocyte proliferation ,Biology ,Virus ,Adenoviridae ,Viral vector ,Cancer immunotherapy ,Neoplasms ,Tumor Cells, Cultured ,medicine ,Humans ,Cytotoxic T cell ,Molecular Biology ,Gene Transfer Techniques ,Cancer ,Immunotherapy ,Cytotoxicity Tests, Immunologic ,Flow Cytometry ,medicine.disease ,Interleukin-12 ,Virology ,Retroviridae ,B7-1 Antigen ,Interleukin 12 ,Molecular Medicine ,Drug Therapy, Combination - Abstract
Synergy between interleukin-12 (IL-12) and B7-1 (CD80) for cancer immunotherapy has previously been demonstrated in animal models of breast cancer, lymphoma, and multiple myeloma. With a view to human clinical application, tricistronic retroviral and adenovirus vectors co-expressing IL-12 (IL-12p40 plus IL-12p35) and CD80 were constructed by utilizing two internal ribosome entry site (IRES) sequences to link the three cDNAs. A murine stem cell virus (MSCV)-based retroviral vector (MSCV-hIL12.B7) utilized distinct IRES sequences from the encephalomyocarditis virus (EMCV) and the foot-and-mouth disease virus (FMCV), whereas Ad5-based adenovirus vectors contained transcriptional units with two EMCV IRES sequences under the control of murine (AdMh12.B7) or human (AdHh12.B7) cytomegalovirus promoters. AdMh12.B7 was found to consistently direct higher levels of IL-12 and CD80 expression than AdHh12.B7 following infection of a number of human tumor cell lines. In preclinical studies, the human myeloma cell line U266 was infected with MSCV-hIL12.B7 and a resulting clonal cell line, U/MSCV-h12.B7, was generated with stable expression of CD80 and secreting IL-12 at 1 ng/24 h/106 cells. By comparison, following AdMh12.B7 infection, 81% of infected U266 cells (U/AdMh12.B7) expressed CD80 and secreted IL-12 at 25–50 ng/24 h/106 cells. Both engineered myeloma cell lines stimulated enhanced allogeneic mixed lymphocyte proliferation and provoked increases in cytotoxic T-lymphocyte responses and γ-interferon release from normal donor lymphocytes exposed to parental U266 cells. These results suggest potential clinical utility of AdMh12.B7 in immunotherapy strategies for the treatment of multiple myeloma and other cancers. Cancer Gene Therapy (2001) 8, 361–370
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- 2001
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10. Diagnosis and surgical treatment of solid pseudopapillary neoplasm of the pancreas: analysis of 24 cases
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Ning Guo, Sheng Q Zou, Zhi H. Li, Ru F. Chen, Qing Lin, Quan B. Zhou, Ji S. Chen, and Jie Wang
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Adult ,Male ,medicine.medical_specialty ,Abdominal pain ,Adolescent ,medicine.medical_treatment ,Context (language use) ,Young Adult ,Pancreatectomy ,Postoperative Complications ,medicine ,Carcinoma ,Humans ,Young adult ,Child ,Pancreas ,Retrospective Studies ,business.industry ,General surgery ,Research ,Retrospective cohort study ,Middle Aged ,medicine.disease ,Carcinoma, Papillary ,Pancreatic Neoplasms ,medicine.anatomical_structure ,Treatment Outcome ,Surgery ,Female ,Radiology ,medicine.symptom ,Presentation (obstetrics) ,business - Abstract
Background Our aim was to summarize our experience with the diagnosis and surgical treatment of solid pseudopapillary neoplasm (SPN) of the pancreas to provide a reference for the management of this rare condition. Methods We collected and analyzed retrospective data on the clinical presentation, laboratory investigations, radiologic imaging, pathology and operative details of patients with SPN of the pancreas diagnosed between February 2001 and December 2009. Results In all, 23 of 24 patients were women, and the mean age of all patients was 31 years. The most common clinical presentation was vague abdominal pain. Abdominal imaging showed solid or solid cystic masses in the pancreas, mostly in the tail or head of the gland. All patients were treated surgically. There were no postoperative deaths. After follow-up ranging from 4 to 109 months (median 68 mo), 20 of 22 patients who underwent curative resection were alive with no evidence of disease recurrence. Of the 2 patients with R1 resections, 1 died 42 months after surgery, whereas the other underwent a second operation and was alive after 36 months' follow-up. Conclusion Solid pseudopapillary neoplasm of the pancreas is a relatively indolent tumour. The initial diagnosis of SPN of the pancreas is suggested by radiologic imaging findings but should be considered in the context of clinical and histopathologic characteristics. We advocate for complete surgical resection once SPN is diagnosed.
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- 2011
11. Maximum length sequence brainstem auditory evoked response in low-risk late preterm babies
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Ze D. Jiang, Chao Chen, Zhi H. Li, and Andrew R. Wilkinson
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Male ,Risk ,medicine.medical_specialty ,Term Birth ,Maximum length sequence ,Gestational Age ,Audiology ,Neonatal Screening ,Late preterm ,Evoked Potentials, Auditory, Brain Stem ,Medicine ,Humans ,business.industry ,Perinatal complications ,Infant, Newborn ,Obstetrics and Gynecology ,Head circumference ,Very preterm ,Auditory brainstem response ,Acoustic Stimulation ,Case-Control Studies ,Pediatrics, Perinatology and Child Health ,Linear Models ,Gestation ,Female ,Brainstem ,business ,Infant, Premature ,Brain Stem - Abstract
Recent research indicates that there is delayed development in the more central part of the auditory brainstem in very preterm babies. We aimed to study whether this is also the case for late preterm babies.The maximum length sequence brainstem auditory evoked response (MLS BAER) was used to study functional status of the auditory brainstem. Babies born at 33-36 week gestation and without any major perinatal complications were recruited. MLS BAER was recorded and analyzed at term age.No significant correlation was found between most MLS BAER variables and physiological factors (gender, postconceptional age, bodyweight, and head circumference obtained at time of testing). Wave latencies and amplitudes, and I-V and I-III intervals in the preterm babies were essentially similar to those in the term controls at all click rates. However, III-V interval increased significantly at 227-910/s clicks (p0.05-0.01). All latencies, amplitudes and intervals correlated significantly with click rates (all p0.001). No differences were found in the slopes of MLS BAER variables-rate functions between the later preterm babies and term controls.Babies born at 33-36 weeks gestation without major complications had an increased III-V interval at high-rate stimulation. This suggests that late preterm babies have a mild delay in neural conduction in the more central part of the auditory brainstem.
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- 2010
12. MOLECULAR CHARACTERIZATION AND EXPRESSION OF THE P38 MAPK GENE FROM THE RIDGETAIL WHITE PRAWN, PALAEMON CARINICAUDA HOLTHUIS, 1950 (DECAPODA, PALAEMONIDAE).
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BEI XUE, PEI ZHANG, ZHI H. LI, LIAN ZHAO, XIAO F. LAI, JIAN H. CHEN, HUAN GAO, and BIN L. YAN
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PALAEMONIDAE ,CRUSTACEAN classification ,CRUSTACEAN morphology ,CRUSTACEAN genetics ,PROTEIN kinases ,CELLULAR signal transduction ,INVERTEBRATES - Abstract
Copyright of Crustaceana is the property of Brill Academic Publishers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
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13. Emerging Issues Questioning the Current Treatment Strategies for Lumbar Disc Herniation
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Zhong Y. Wan, Hua Shan, Tang F. Liu, Fang Song, Jun Zhang, Zhi H. Liu, Kun L. Ma, and Hai Q. Wang
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adjacent segment disease ,instrumentation ,lumbar disc herniation ,lumbar fusion ,metallosis ,Surgery ,RD1-811 - Abstract
Lumbar disc herniation is among the common phenotypes of degenerative lumbar spine diseases, significantly affecting patients' quality of life. The practice pattern is diverse. Choosing conservative measures or surgical treatments is still controversial in some areas. For those who have failed conservative treatment, surgery with or without instrumentation is recommended, causing significant expenditures and frustrating complications, that should not be ignored. In the article, we performed a literature review and summarized the evidence by subheadings to unravel the cons of surgical intervention for lumbar disc herniation. There are tetrad critical issues about surgical treatment of lumbar disc herniation, i.e., favorable natural history, insufficient evidence in a recommendation of fusion surgery for patients, metallosis, and implant removal. Firstly, accumulating evidence reveals immune privilege and auto-immunity hallmarks of human lumbar discs within the closed niche. Progenitor cells within human discs further expand the capacity with the endogenous repair. Clinical watchful follow-up studies with repeated diagnostic imaging reveal spontaneous resolution for lumbar disc herniation, even calcified tissues. Secondly, emerging evidence indicates long-term complications of lumbar fusion, such as adjacent segment disease, pseudarthrosis, implant failure, and sagittal spinal imbalance, which get increasing attention. Thirdly, systemic and local reactions (metallosis) for metal instrumentation have been noted with long-term health concerns and toxicity. Fourthly, the indications and timing for spinal implant removal have not reached a consensus. Other challenging issues include postoperative lumbar stiffness. The review provided evidence from a negative perspective for surgeons and patients who attempt to choose surgical treatment. Collectively, the emerging underlying evidence questions the benefits of traditional surgery for patients with lumbar disc herniation. Therefore, the long-term effects of surgery should be closely observed. Surgical decisions should be made prudently for each patient.
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- 2022
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14. Subject Index Vol. 7, 2007
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R.K. Vasishta, L. Bertacca, Daniel H. Palmer, Maria A. Caligo, Jie Wang, Alessandro Zerbi, Martin E. Fernandez-Zapico, Marco Del Chiaro, Hein G. Gooszen, David N. Criddle, Nora Sartori, Roberto Salvia, Fen Y. Yu, Gwen Lomberk, Giorgio Casari, Gulsen Yilmaz, Leticia de la Cueva, Philip R. Fischer, Valerio Di Carlo, Laura Seynaeve, James M. Scheiman, Osman Yüksel, Quan B. Zhou, H. Riess, John A. Dumot, Michelle A. Anderson, Daniel D. Billadeau, Timothy A. Woodward, H. Oettle, P. Neuhaus, Raffaele Pezzilli, Raul Urrutia, Kyung W. Noh, Ömer Başar, Silvano Presciuttini, Massimo Falconi, María Jesús Martínez, Fernando Gómez, Alexander S. Gordetzov, Franco Mosca, Marc G. Besselink, Jia J. Zhou, Marco Polese, Mehmet Ibis, Ji S. Chen, J. Krysa, Steven D. Freedman, Fatma Meric Yilmaz, Sandeep Nijhawan, Massimo Raimondo, R. Sopena, Euan McLaughlin, Qi B. Tang, Maxim S. Petrov, Ole H. Petersen, Matthew J. DiMagno, Eduardo Moreno-Osset, Lorenzo Fantini, D.K. Bakshi, A. Steger, Mikhail V. Kukosh, Paolo Pederzoli, J. A. Murphy, Zeynel Abidin Öztürk, Tomás Ripollés, L. Singh, S. Majumdar, Werner Van Steenbergen, Mark J. Truty, Felipe Fregni, Seyfettin Köklü, Ru F. Chen, Joel H. Rubenstein, Ricardo A. Cruciani, Surakit Pungpapong, Qiu H. Pan, Michael B. Wallace, Annemarie C. de Vries, Maria Schipper, Biancamaria Longoni, Mohammad A. Al-Haddad, György M. Buzás, Zhi H. Li, S.K. Arora, Ben U. Ridwan, Pankaj Jain, Robert Sutton, J.D. Wig, Alvaro Pascual-Leone, Karel J. van Erpecum, Erik Buskens, Ugo Boggi, Antonio López, Emre Yıldırım, Mansour A. Parsi, Madhusudhan R. Sanaka, and Antonio Maria Morselli-Labate
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Index (economics) ,Hepatology ,business.industry ,Endocrinology, Diabetes and Metabolism ,Statistics ,Gastroenterology ,Medicine ,Subject (documents) ,business - Published
- 2007
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