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To explore the feasibility of constructing a functional biomaterial complex with regenerated silk fibroin membrane and immortalized chondrocytes in vivo. Rat auricular chondrocytes (RACs) were transfected with the lentivirus vector pGC-FU-hTERT-3FLAG or pGC-FU-GFP-3FLAG, encoding the human telomerase reverse transcriptase (hTERT) or GFP gene. The effects of regenerated silk fibroin film on the adhesion, growth of immortalized chondrocytes and expression of collagen II in vitro were analyzed with immunofluorescent histochemistry. Immortalized RACs were transformed. Induction by nutrient medium promoted higher expression levels of collagen II in transformed chondrocytes. The regenerated silk fibroin film was not cytotoxic to immortalized chondrocytes and had no adverse influence on their adhesion. Collagen II expression was good in the immortalized chondrocytes in vivo. The construction of a silk-based biomaterial complex with immortalized chondrocytes may provide a feasible kind of functional biomaterial for the repair of cartilage defects in clinical applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1071–1078, 2014.

To explore the feasibility of constructing a functional biomaterial complex with regenerated silk fibroin membrane and immortalized chondrocytes in vivo. Rat auricular chondrocytes (RACs) were transfected with the lentivirus vector pGC-FU-hTERT-3FLAG or pGC-FU-GFP-3FLAG, encoding the human telomerase reverse transcriptase (hTERT) or GFP gene. The effects of regenerated silk fibroin film on the adhesion, growth of immortalized chondrocytes and expression of collagen II in vitro were analyzed with immunofluorescent histochemistry. Immortalized RACs were transformed. Induction by nutrient medium promoted higher expression levels of collagen II in transformed chondrocytes. The regenerated silk fibroin film was not cytotoxic to immortalized chondrocytes and had no adverse influence on their adhesion. Collagen II expression was good in the immortalized chondrocytes in vivo. The construction of a silk-based biomaterial complex with immortalized chondrocytes may provide a feasible kind of functional biomaterial for the repair of cartilage defects in clinical applications.

Qualitative simulation is effective methods processing incomplete knowledge, how to represent qualitative knowledge and reduce spurious behaviors is its research emphasis. Contraposing the limitations of existing methods representing qualitative knowledge and filtering spurious behaviors, an approach is proposed to qualitatively model and simulate on the basis of normal cloud model. In this research, the cloud numeric characteristics and cloud measurement are introduced into this approach; consequently, it can more accurately assess the qualitative knowledge, and expand the variables expression and quantity space; meanwhile, it is constructed the means of modeling and simulation by adopting more amply portraying the system status, more intuitively transforming status and more accurately filtering spurious behaviors. Furthermore, in simulation algorithm, cloud measurement indices are employed to significantly select the successor status which can not be determined by existing constraint means. The results of simulation example demonstrate that our approach effectively improves the ability to filter spurious behaviors, and it is a feasible method.

Based on the QSIM reasoning of Kuipers, comparative constraint is presented to reduce the diagnosis space. The transfer regulation of qualitative constrain is used in the simulation and reasoning of system diagnosis. This arithmetic takes observed faulty state as the beginning, to diagnosis the discrepancy of variable and location of the causation according to the transfer regulation of qualitative constraint, reasons from faulty diagnosed to examine the result and delete the redundancy of diagnosis result. In the example of Condensation refrigeration system, the constraint relation is built according to qualitative difference equation. Aim at the bad efficiency of refrigeration, the mixture of air or Freon superfluously is diagnosed as the result faulty source, is consistent with the factual system.