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Subarachnoid hemorrhage (SAH) is a devastating stroke caused by ruptured intracranial aneurysms, leading to blood accumulation around the brain. Early brain injury (EBI) within 72 h post-SAH worsens prognosis, primarily due to intense neuroinflammation. Microglia, pivotal in central nervous system defense and repair, undergo M1 to M2 polarization post-SAH, with M1 exacerbating neuroinflammation. Propofol (PPF), an anesthetic with anti-inflammatory properties, shows promise in mitigating neuroinflammation in SAH by modulating microglial activation. It likely acts through microRNAs like miR-140-5p, which attenuates microglial activation and inflammation by targeting TREM-1 and the NF-κB pathway. Understanding these mechanisms could lead to new therapeutic approaches for SAH-related EBI. In this study, BV-2 cell was used to establish in vitro model of SAH, and the expression of miR-140-5p and TREM-1 was detected after modeling. Microglial activity, apoptosis, the inflammatory pathway and response, oxidative damage, and M1/M2 polarization of microglia were evaluated by drug administration or transfection according to experimental groups. Finally, the targeting relationship between miR-140-5p and TREM-1 was verified by dual luciferase reporter assays, and the effect of PPF on the miR-140-5p/TREM-1/NF-κB signaling cascade was evaluated by RT‒qPCR or Western blotting. PPF effectively mitigates apoptosis, neuroinflammation, oxidative damage, and M1 microglial polarization in SAH. In SAH cells, PPF upregulates miR-140-5p and downregulates TREM-1. Mechanistically, PPF boosts miR-140-5p expression, while TREM-1, a downstream target of miR-140-5p, inhibits NF-κB signaling by regulating TREM-1, promoting M1 to M2 microglial polarization. Reduced miR-140-5p or increased TREM-1 counters PPF's therapeutic impact on SAH cells. In conclusion, PPF plays a neuroprotective role in SAH by regulating the miR-140-5p/TREM-1/NF-κB signaling axis to inhibit neuroinflammation and M1 polarization of microglia.

The West Qinling Orogenic Belt (WQOB) in central China records the tectonic evolution and deep geodynamics process associated with plate collision on the northeastern margin of the Qinghai–Tibet Plateau. The study of the deep–seated West Qinling structure is beneficial for revealing the evolution of the West Qinling crust during the continental collision orogeny, and also has significance for mineral exploration. In this study, the crustal structure and the distribution of major faults in the West Qinling Orogen are calculated by processing geophysical aeromagnetic and ground gravity data. The density and magnetic susceptibility differences between the West Qinling crust and the upper mantle are calculated by fitting, and the depth and trend of the faults related are inferred from the gravity and magnetic inversion results. Due to the overall subduction of the lower crust of the Ruoergai to the West Qinling Orogenic Belt, the structural deformation within the West Qinling is strong, and the geophysical magnetic field and gravity field are suddenly changed. It is indicated that the formation of the Diebu–Sanhe fault may have a strong correlation with the Mianlue suture zone, and their intersection may penetrate into the lower crust at a depth of about 50 km or more. The West Qinling structure represents important conduits for migrating magmatic–derived hydrothermal fluids. During the continental collision, the West Qinling major faults provided hydrothermal migration channels for ore–forming materials in different tectonic periods. Meanwhile, multiple the geological activities led to the formation of mineral deposits related to magmatic hydrothermal fluids.

This paper aims to achieve bedrock geologic mapping in the overburden area using big data, distributed computing, and deep learning techniques. First, the satellite Bouguer gravity anomaly with a resolution of 2′×2′ in the range of E66°-E96°, N40°-N55° and 1:5000000 Asia-European geological map are used to design a dataset for bedrock prediction. Then, starting from the gravity anomaly formula in the spherical coordinate system, we deduce the non-linear functional between rock density ρ and rock mineral composition m, content p, buried depth h, diagenesis time t and other variables. We analyze the feasibility of using deep neural network to approximate the above nonlinear generalization. The problem of solving deep neural network parameters is transformed into a non-convex optimization problem. We give an iterative, gradient descent-based solution algorithm for the non-convex optimization problem. Utilizing neural architecture search (NAS) and human-designed approach, we propose a geological-geophysical mapping network (GGMNet). The dataset for the network consists of both gravity anomaly and a priori geological information. The network has fast convergence speed and stable iteration during the training process. It also has better performance than a single neural network search or human-designed architectures, with the mean pixel accuracy (MAP) = 63.1% and the frequency weighted intersection over union (FWIoU) = 42.88. Finally, the GGMNet is used to predict the rock distribution of the Junggar Basin.

Objectives: Endothelial-to-mesenchymal transition (EndoMT) is a significant biological phenomenon wherein endothelial cells undergo a loss of their endothelial traits and progressively acquire mesenchymal characteristics. Consequently, this transformation leads to both a compromised ability to maintain lumen permeability and alterations in vascular structure, which hampers the preservation of blood-brain barrier integrity. This study aimed to investigate inflammation-induced EndoMT and its etiology, with the goal of impeding the infiltration of peripheral inflammation into the central nervous system. Materials and Methods: Lipolysaccharide (LPS) was administered intraperitoneally to mice several times to establish a chronic inflammatory model. A cellular inflammatory model was established by LPS in human brain microvascular endothelial cells (HBMECs). The mRNA expressions of inflammatory cytokines interleukin-1β (IL-1β) and IL-6 were detected by real-time polymerase chain reaction (PCR). Immunofluorescence staining of platelet endothelial cell adhesion molecule-1 (CD31) and alpha smooth muscle actin (α-SMA) was conducted to assess the level of EndoMT. The expression levels of Occludin, zona occludens protein 1 (ZO-1), Sestrin2, microtubule-associated protein1 light chain 3 (LC3) and inducible nitric oxide synthase (iNOS) were detected by western blotting. Results: LPS treatment induced the downregulation of ZO-1 and Occludin, which was accompanied by the elevated expressions of iNOS, α-SMA, Sestrin2 and LC3-II in the mouse cortex and HBMECs. Mechanistically, the knockdown of Sestrin2 in HBMECs exacerbated the EndoMT induced by LPS treatment, while the overexpression of Sestrin2 inhibited this process. Moreover, the induction of autophagy by rapamycin rescued the EndoMT induced by Sestrin2 knockdown. Conclusion: This study revealed that Sestrin2 inhibited endothelial inflammation and EndoMT via enhanced autophagy, which may provide a potential drug target for cerebrovascular inflammatory injury.

Abstract Background As a common disorder of the gastrointestinal tract, irritable bowel syndrome (IBS) can have negative effects on patients and society, with irritable bowel syndrome with constipation(IBS-C) accounting for a large proportion of these effects. The main clinical manifestations of IBS-C are constipation, abdominal pain, and abdominal distension, which seriously impact the quality of life of patients. The mechanisms of IBS are complex, and the gut-brain axis has been an emerging and recognized theoretical system in recent years. Based on the theory of the gut-brain axis and the theory of Chinese medicine, we designed this study to evaluate the efficacy of one-finger meditation massage in treating IBS-C. Methods/design This is a randomized controlled trial. Eligible patients with irritable bowel syndrome (IBS-C) wererandomized 1:1 to a test group (massage plus probiotics) and a control group (probiotics). Patients in the test group weretreated once every 10 days for three consecutive courses of treatment (i.e., three months) and weregiven Bifidobacterium trifolium capsules 630 mg/dose three times daily 30 min after meals every day during the treatment period, with follow-up observations at the end of the third and sixth months of the treatment period. The control group weregiven Bifidobacterium trifolium capsules 630 mg/dose, 3 times a day for 3 months, with follow-up observations at the end of the third and sixth months of the treatment period. The primary outcome indicators are the concentrations of 5-HT and substance P and the IBS Severity Scale (IBS-SSS) assessment. Secondary outcomes are the Bristol Rating Scale (BRSA) score, the IBS Quality of Life Questionnaire (IBS-QOL scale) score, and the assessment of the effectiveness of the evidence. The results wereassessed at the pretreatment, posttreatment, and follow-up stages. Any side effects weresubject to assessment. Discussion The aim of this trial is to provide a new method of treatment based on pharmacological treatment that is easy to use, easy to promote and has proven efficacy and to establish the efficacy and safety of treating IBS-C through this trial. Registration for Trial Chinese Clinical Trial Registry ChiCTR2200066417 on 5 December 2022. https://www.chictr.org.cn/bin/project/edit?pid=183461

Evacuation signs are a key factor in the effectiveness of occupants’ urgent escape from buildings. Different sign features may have disparate impacts on individual and crowd behavior. This study aims to quantitatively investigate the effectiveness of building evacuation signs with different features during pedestrian evacuation using eye-tracking devices. Ten experiments were conducted in a building in T-junction scenarios, and four sets of features related to evacuation signs (color, position, graphics, and flashing) were considered. SMI BeGaze was used to analyze the eye movements of the occupants. The fixation duration of the evacuees and the ratio of signs detected and followed were quantitatively derived and compared for each experiment. The results show that it is easier for evacuees to detect signs at a low sightline, and signs posted at that level of sight can provide better guidance. When signs are posted high up, red signs are easier to detect than green signs. Most evacuees prefer to follow the evacuation signs once they detect them; however, there is no significant difference in the effect of what is posted on the evacuation signs, such as a running man or an arrow. Conversely, flashing of signs is highly helpful in influencing evacuees’ behavior, but flashing is not helpful in detecting signs. More importantly, red signs with a running man graphic located in a low position have the best guiding effects on evacuees. The fixation behaviors of evacuees vary according to sign features, particularly in regard to flashing and color. Evacuees stare longer at twinkling signs. Differences in the colors and positions of signs result in significantly different perceptual behaviors among evacuees. Our findings are useful for building designers and provide guidance for developing effective evacuation strategies.

Terrorist attacks are a catastrophic man-made emergent phenomenon. The current situation of international violent terrorism is severe. Public places with high-density crowds are more likely to be attacked by violent terrorists. To effectively reduce the number of casualties caused by violent terrorist incidents, it is crucial to take preventive management at the beginning of the incident. This paper analyzed the characteristics of the self-organization of crowds and terrorists in public places during a suicide bombing attack and conducted simulation experiments with an agent-based model. The simulation results show that unobstructed escape routes and controlling external sites will improve the evacuation efficiency of the crowd when a violent terrorist incident occurs; the pedestrian flow in public places affects the overall evacuation time; and timely and effective control of violent terrorists can reduce the number of casualties among the crowd. The optimized strategies obtained through experiments will help public security organs prevent violent terrorist incidents in public places and improve the efficiency of early disposal.

Guidance signs are generally considered to be key in improving the efficiency of emergency building evacuations. During a group evacuation, how obedient is the group to the evacuation signs? Would different evacuation signs affect the judgment of evacuated groups? Evacuation experiments were conducted to explore the influence of evacuation signs on the path selection of evacuation groups. Fourteen classes with 514 participants participated in these experiments, and each class participated in one experiment. Six classes conducted the experiments without guidance signs, and the rest conducted the experiments with guidance signs. The influences of the sign's color (red and green), pattern (arrow and running man), and status (whether it flashes) on the route selection were studied. The results show that (1) the evacuation signs significantly affect the route choices of the evacuated groups. (2) The herding effect during the evacuation process does not influence the guiding effect of evacuation signs. (3) Evacuation signs of different colors and patterns have different guiding effects, and the Green running man sign has the best effect. (4) The constant bright, or flashing evacuation sign corresponding to the flashing Red running man has a significant impact on the route selection of the evacuated group. The results may provide a reference for improving the emergency evacuation guidance model of office buildings or other buildings with a large flow of people and optimize ideas for the design and layout of emergency evacuation signs.

Omicron variant of SARS-CoV-2 has become the predominant variant worldwide. VV116 is an oral drug with robust anti-SARS-CoV-2 efficacy in preclinical studies. We conducted an open, prospective cohort study to evaluate its safety and effectiveness in Chinese participants infected with the omicron variant from March 8th, 2022 to March 24th, 2022. 136 hospitalized nonsevere patients confirmed with COVID-19 were enrolled including 60 patients who received VV116 (300 mg, BID×5 days) in the treatment group and 76 patients who didn’t receive VV116 in the control group besides standard treatment. Viral load shedding time and adverse events were collected during the follow-up. There was no significant difference in baseline characteristics between the VV116 group and the control group, except for a higher symptom prevalence in the control group (P = 0.021). The median time from the first positive test to the first VV116 administration was 5 (range: 2-10) days. Participants who received VV116 within 5 days since the first positive test had a shorter viral shedding time than the control group (8.56 vs 11.13 days), and cox regression analysis showed adjusted HR of 2.37 [95%CI 1.50-3.75], P

AbstractWRKY proteins are a class of transcription factors involved in plant growth and development, biotic and abiotic stress, and other biological processes. In order to systematically study the structure and function of WRKY proteins in cabbage, we used the whole genome sequence of cabbage in the Brassica database (BRAD) and bioinformatics methods. A total of 150 WRKY genes were identified in cabbage, named BolWRKY1-BolWRKY150. These genes were unevenly distributed on 9 chromosomes. In this study, the WRKY proteins were classified into three classes: I, II and III. Cis-acting elements analysis showed that the BolWRKY gene was involved in multiple hormone and stress responses. The protein interaction network analysis suggested that BolWRKY19/100/105 genes might play similar functions in the same stress response. Based on transcriptome data analysis, we found that different BolWRKY genes showed tissue-specific expression. Quantitative real-time PCR analysis showed that several BolWRKY genes were strongly induced by drought stress and ABA (abscisic acid) treatment, indicating members of BolWRKY gene family were closely related to the stress responses such as drought. Our study lays a foundation for further understanding the regulatory mechanism and function of the WRKY gene in response to abiotic stress.

The evaluation simulation of urban green space landscape planning scheme based on PSO-BP neural network model is carried out in this paper. PSO-BP neural network can combine the principle of landscape ecology, integrate more evaluation indicators of ecology and urban development into the urban green space landscape planning scheme, and simply understand and predict human behavior, so as to make a more comprehensive evaluation and prediction of the urban green space landscape planning scheme. It not only has superior memory storage and learning ability, but also can simply understand and predict human behavior, so that more influencing factors that cannot be added in the past can be considered in the scheme evaluation and analysis, and the evaluation of urban green space landscape planning scheme is more comprehensive, scientific and reasonable. Experiments show that PSO-BP neural network has smaller error and better generalization ability than BP neural network. PSO-BP neural network rating model can analyze its more reasonable proportion according to the relationship between different types of green space and indicators, and give corresponding adjustment suggestions, which has guiding significance for the modification and adjustment of urban green space landscape planning scheme.

Land type survey is an important task of land resources survey and the basis of scientific management of land resources. With the increasingly prominent problems of population, resources, and environment, there is an urgent need for a fast and accurate classification method of large-scale land use and land cover based on remote sensing data. Traditional machine learning classification methods based on pixel classification achieved sufficient results and are widely used, such as maximum likelihood classification and random forests method. However, with the development of the novel technology of deep learning, in practical application, for multi-classified land resources, how to use the fast and effective classification method of low and medium resolution RS images needs further research. This paper takes the land resource classification of the Tonghe medium resolution RS dataset of the third land survey in China as an example to screen and compare traditional machine learning classification methods and semantic segmentation models FC-DenseNet56, GCN, BiSeNet, U-Net, DeepLabV3, AdapNet, and PSPNet, which aim to select the optimal feature extraction model. The results show that the classification accuracy of the U-Net model can reach 93.62%, which is more accurate and effective than traditional machine learning methods and other semantic segmentation models. It is suitable for multi-classification tasks of land cover resources in low and medium resolution RS images and shows a superior effect in practical application. Besides, the conclusion of this study can provide a demonstration for large-scale land cover resources investigation using low and medium resolution RS images.

The gravity gradient tensor, which has a higher resolution than gravity, is used in a variety of fields, including the discovery of energy resources, auxiliary navigation, and national defense building. Our team has achieved significant advancements in various essential technologies, such as high-resolution accelerometers, and has constructed China’s first self-controllable shipboard gravity gradient measurement system. In the laboratory, accuracy is determined using the mass gravitation technique, static test accuracy of Tuv and Txy is 7.22 E and 3.58 E, while dynamic test accuracy of Tuv and Txy is 9.09 E and 4.16 E. For outfield shipborne test measurement, the internal accord accuracy of Tuv and Txy of the repeat line is 28.2E@750m and 28.8E@750m, and that of the intersection point is 28.2E@750m and 26.8E@750m. The performance of the system is completely validated by dynamic and static testing, laying the groundwork for the practical implementation of gravity gradient technology.

Based on the blasting seismic detection data obtained in the southeast of North China in recent years, this paper comprehensively analyzes and studies the crust-mantle lithospheric structure and seismological characteristics of different tectonic regions, such as offshore basins, west Shandong uplift, Tanlu fault zone and Jiangsu-Shandong orogenic belt. The low-velocity Pg waves in Dongying depression and Northern Jiangsu basin reveal the unstable basement structure with extremely thick sediments. The travel time of Pg wave is characterized by relatively low propagation velocity and small crustal thickness of offshore continental margin; the first break time and high apparent velocity of Pg wave in west Shandong uplift indicate that the sedimentary basement is relatively thin. The Pm wave shows the characteristic of dominant wave in the first-order velocity discontinuity of the crust-mantle interface, which reflects the high crustal velocity and stable structure in west Shandong uplift. The Pm and Pl wave are obviously complicated, which can reflect the crust-mantle lithospheric structure of the transitional zone between Tanlu fault zone and Jiangsu-Shandong orogenic belt. The small time difference between Pn and PL waves can be regarded as the highly destructive seismological manifestation of Tanlu fault zone on the crust-lithosphere scale. Based on many geophysical phenomena such as electrical structure, density structure and terrestrial heat flow, it is believed that the lithospheric destruction degree of Tanlu fault zone and Jiangsu-Shandong orogenic belt was high during the destruction of the North China Craton.

Non-human primates (NHP) share a close relationship with humans due to a genetic homology of 75–98.5%. NHP and humans have highly similar tissue structures, immunity, physiology, and metabolism and thus often can act as hosts to the same pathogens. Agriculture, meat consumption habits, tourism development, religious beliefs, and biological research have led to more extensive and frequent contact between NHPs and humans. Deadly viruses, such as rabies virus, herpes B virus, Marburg virus, Ebola virus, human immunodeficiency virus, and monkeypox virus can be transferred from NHP to humans. Similarly, herpes simplex virus, influenza virus, and yellow fever virus can be transmitted to NHP from humans. Infectious pathogens, including viruses, bacteria, and parasites, can affect the health of both primates and humans. A vast number of NHP-carrying pathogens exhibit a risk of transmission to humans. Therefore, zoonotic infectious diseases should be evaluated in future research. This article reviews the research evidence, diagnostic methods, prevention, and treatment measures that may be useful in limiting the spread of several common viral pathogens via NHP and providing ideas for preventing zoonotic diseases with epidemic potential.

The deformation behavior of metal materials in plastic forming is intimately related to deformation conditions, which are greatly affected by deformation rate, forming temperature, and plastic variables. Macroscopic mechanical properties research is an important basis and technical means to analyze the process parameters and deformation process of metal plastic forming. Therefore, to reveal the influence mechanism of macroscopic mechanical properties of metal materials, and establish material constitutive models under different deformation conditions, it is of great significance to choose reasonable forming parameters and prevent forming defects. There are substantial variances in the macroscopic mechanical characteristics of different materials in the deformation process. In order to accurately predict its deformation behavior, the phenomenological constitutive model, the microscopic constitutive model reflecting the microscopic deformation mechanism, and the artificial neural network constitutive model based on the neural network were constructed respectively on the basis of macroscopic mechanical tests and microscopic microstructure tests. On the basis of the existing research results, the advantages and disadvantages of phenomenological constitutive model, microscopic constitutive model, and neural network constitutive model are compared and analyzed, respectively. The research results of this paper will provide support for the selection of constitutive models for reasonably predicting the deformation behavior of metal materials.

The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation and resorption activities. Notably, B-cell–derived exosomes inhibited bone homeostasis in fracture healing. In humans, a decrease in the number of B cells during the epiphyseal phase stimulated fracture healing. Then, as the numbers of osteoblasts increased during the callus reconstruction stage, the number of B cells gradually recovered, which reduced additional bone regeneration. Thus, B cells are key regulators of fracture healing and inhibit excessive bone regeneration by producing multiple osteoblast inhibitors.

Anthocyanins are among the most important factors affecting the leaf colour of kale, and MYB proteins are key transcription factors regulating their synthesis in plants. In this study, purple-leaf kale ‘D07’ was used as a test material, and the complete coding sequence of BoMYB was determined by homologous cloning. Bioinformatics analysis showed that the cDNA sequence of the BoMYB coding region was 753 base pairs in length and encoded a predicted 250-amino acid protein with a mass of 25 kDa and an isoelectric point of 9.08. The BoMYB protein was predicted to contain two MYB conserved domains and to be localized in the cell nucleus. A phylogenetic tree indicated that the amino acid sequence encoded by BoMYB in kale was most closely related to the MYB protein sequence of Brassica napus. The expression patterns of BoMYB were evaluated in different tissues of ‘D07’ by quantitative real-time polymerase chain reaction, and the results showed that BoMYB was expressed in kale roots, stems, inner leaves, and outer leaves. However, the expression levels were tissue specific and correlated with the anthocyanin content in each tissue. To confirm the effect of BoMYB on anthocyanin synthesis and accumulation, ectopic expression in Arabidopsis was performed. Morphological observation showed that overexpression of BoMYB increased the accumulation of anthocyanidins in transgenic Arabidopsis. Thus, BoMYB may encode an MYB transcription factor, which positively regulates the synthesis of anthocyanins in kale.

Baicalin is a natural flavonoid glycoside that confers protection against myocardial ischemia/reperfusion (I/R) injury. However, its mechanism has not been fully understood. This study focused on elucidating the role of ferroptosis in baicalin-generated protective effects on myocardial ischemia/reperfusion (I/R) injury by using the myocardial I/R rat model and oxygen–glucose deprivation/reoxygenation (OGD/R) H9c2 cells. Our results show that baicalin improved myocardial I/R challenge–induced ST segment elevation, coronary flow (CF), left ventricular systolic pressure , infarct area, and pathological changes and prevented OGD/R-triggered cell viability loss. In addition, enhanced lipid peroxidation and significant iron accumulation along with activated transferrin receptor protein 1 (TfR1) signal and nuclear receptor coactivator 4 (NCOA4)-medicated ferritinophagy were observed in in vivo and in vitro models, which were reversed by baicalin treatment. Furthermore, acyl-CoA synthetase long-chain family member 4 (ACSL4) overexpression compromised baicalin-generated protective effect in H9c2 cells. Taken together, our findings suggest that baicalin prevents against myocardial ischemia/reperfusion injury via suppressing ACSL4-controlled ferroptosis. This study provides a novel target for the prevention of myocardial ischemia/reperfusion injury.

The aim of this study is to investigate the role of microRNA-499 (miR-499) in hepatocellular carcinoma tumor growth and the underlying molecular mechanisms. The expression of miR-499 was significantly decreased in hepatocellular carcinoma tissues compared with that in adjacent normal tissues. Furthermore, miR-499 overexpression in HEPG2 cell was related to the tumor growth in nude mice xenograft models. Likewise, miR-499 mimic or inhibitor decreased or accelerated cell proliferation, respectively. Mechanistically, miR-499 directly targeted the 3′- untranslated region of astrocyte elevated gene-1 and downregulate astrocyte elevated gene-1 expression. Restoration of astrocyte elevated gene-1 expression in hepatocellular carcinoma cells reversed the inhibitory effect of miR-499 on cell growth. In addition, astrocyte elevated gene-1 and miR-499 expression were inversely correlated in human and mice hepatocellular carcinoma tissues. Our study identified miR-499 as a tumor-suppressive miR in hepatocellular carcinoma, thus providing a candidate therapeutic target for the future diagnosis or treatment of hepatocellular carcinoma.

Since the Mesozoic, southeastern North China Craton has experienced intense crustal thinning and lithosphere destruction. Some of the responses of the deep activity in the upper crust crystalline basement have been retained in a series of tectonic evolution. The study of the upper crust velocity structure, especially the properties of the basement interface, is of great significance for studying the tectonic evolution and seismic hazard in the southeastern part of North China. In this study, we selected Pg waves of the blasting seismic data in the southeastern part of North China in recent years, which reflect the west Shandong uplift, offshore sedimentary basins and the Tanlu Fault zone and the Sulu orogenic transition zone, to study the structural and seismological characteristics of basement in North China Craton. The results of this study showed as follows: First, the obvious lag of Pg wave arrival time in Dongying depression and North Jiangsu basin reveals the thick sedimentary, low velocity and unstable basement structure. Second, the advance Pg wave arrival time with high apparent velocity, which reflects the basement structure of the west Shandong uplift, indicates the thin sediments and the shallow basement. Third, combined with many geophysical phenomena, such as electrical structure, density structure and terrestrial heat flow, we hold that the Tanlu tectonic belt and the Sulu orogenic belt have experienced great lithosphere destruction and there is shallow crust and the thinnest lithosphere in the vicinity of the Tanlu fault zone. Keywords: Blasting seismic detection, Southeastern North China, Crystalline basement, Pg arrival time

As a relatively new method of processing non-stationary signal with high time-frequency resolution, S transform can be used to analyze the time-frequency characteristics of seismic signals. It has the following characteristics: its time-frequency resolution corresponding to the signal frequency, reversible inverse transform, basic wavelet that does not have to meet the permit conditions. We combined the threshold method, proposed the S-transform threshold filtering on the basis of S transform time-frequency filtering, and processed airgun seismic records from temporary stations in “Yangtze Program” (the Anhui experiment). Compared with the results of the bandpass filtering, the S transform threshold filtering can improve the signal to noise ratio (SNR) of seismic waves and provide effective help for first arrival pickup and accurate travel time. The first arrival wave seismic phase can be traced farther continuously, and the Pm seismic phase in the subsequent zone is also highlighted. Keywords: S transform, Time-frequency filtering, Airgun data, Threshold filtering, De-noising

Soil salinisation is a growing threat to global agriculture, reducing crop yields. Brassicaceae crops are vital vegetables and cash crops. Salt stress significantly affects the growth and development of Brassicaceae crops. A better understanding of the molecular and physiological mechanisms of salt tolerance is of theoretical and practical importance to improve Brassicaceae crop's salt tolerance and crop quality. Combined with previous research results, we discuss recent advances in research on salt stress response and salt tolerance in Brassicaceae crops. We summarised recent research progress on the physiological and molecular mechanisms of ionic homeostasis, antioxidant regulation, hormonal regulation and accumulation of osmotic-adjustment substances. We also discussed the molecular mechanism of Brassicaceae crop salt tolerant varieties from the perspective of differentially expressed genes, differentially expressed proteins and metabolites through transcriptome, proteome and metabonomic analysis methods. This paper summarises the molecular mechanisms in the perspective of differentially expressed genes, differentially expressed proteins, and metabolites through transcriptomic, proteome and metabolomics analysis. The review provides abundant data for accelerating the breeding of salt-tolerant Brassicaceae and laid a foundation for understanding the mechanism of salt tolerance of Brassicaceae crops and breeding salt-tolerance varieties. [ABSTRACT FROM AUTHOR]

Abstract Fungi from the Pyricularia genus cause blast disease in many economically important crops and grasses, such as wheat, rice, and Cenchrus grass JUJUNCAO. Structure variation associated with the gain and loss of effectors contributes largely to the adaptive evolution of this fungus towards diverse host plants. A telomere-to-telomere genome assembly would facilitate the identification of genome-wide structural variations through comparative genomics. Here, we report a telomere-to-telomere, near-complete genome assembly of a Pyricularia penniseti isolate JC-1 infecting JUJUNCAO. The assembly consists of eight core chromosomes and two supernumerary chromosomes, named mini1 and mini2, spanning 42.1 Mb. We annotated 12,156 protein-coding genes and identified 4.54% of the genome as repetitive sequences. The two supernumerary chromosomes contained fewer genes and more repetitive sequences than the core chromosomes. Our genome and results provide valuable resources for the future study in genome evolution, structure variation and host adaptation of the Pyricularia fungus.

BACKGROUND: Electroacupuncture (EA) is a promising alternative therapy for migraine, with mitochondrial dysfunction hypothesized as a pivotal mechanism in migraine pathophysiology. This research endeavors to investigate the therapeutic potential of EA in addressing migraines and shed light on the associated mechanisms linked to mitochondrial anomalies. MATERIALS AND METHODS: Migraine in rats was induced by 10 mg/kg nitroglycerin, followed by 2/15 Hz EA treatment at GB20 and LR3. Nociceptive behavior was recorded via a camera and analyzed using EthoVision XT 12.0 software. The hind-paw withdrawal threshold was assessed using the von Frey test. We assessed the levels of calcitonin gene-related peptide (CGRP), nitric oxide (NO), and endothelin (ET) – key parameters in migraine pathophysiology using immunohistochemistry and enzyme-linked immunosorbent assay. Mitochondrial morphology in brain tissues was observed through transmission electron microscopy. Reactive oxygen species (ROS) level in mitochondria was measured by flow cytometry. The levels of PINK1 and Parkin were assessed using Western blot analysis. RESULTS: EA at GB20 and LR3 decreased nociceptive behaviors (resting and grooming) and increased exploratory and locomotor behaviors in migraine rats. The hind-paw withdrawal threshold in migraine rats was significantly elevated following EA treatment. Post-EA treatment, levels of CGRP and NO decreased, while ET level increased, suggesting an alteration in pain and vascular physiology. Notably, EA treatment mitigated the mitochondrial damage and reduced ROS level in the brain tissues of migraine rats. EA treatment upregulated the expression of PINK1 and Parkin in migraine rats. CONCLUSION: EA at GB20 and LR3 may treat migraine by alleviating PINK1/Parkin-mediated mitochondrial dysfunction.