1. Single-cell-resolution transcriptome map of human, chimpanzee, bonobo, and macaque brains
- Author
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Malgorzata Santel, Song Guo, Barbara Treutlein, Shen Rong, J. Gray Camp, Anna Tkachev, Marat Sabirov, Ilia Kurochkin, Pavel V. Mazin, Philipp Khaitovich, Matvei Bulat, Svante Pääbo, Dingding Han, Chet C. Sherwood, Olga Efimova, Sabina Kanton, Patricia Guijarro, Zhengzong Qian, and Ekaterina Khrameeva
- Subjects
Resource ,Pan troglodytes ,RNA-Seq ,Macaque ,Evolution, Molecular ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Single-cell analysis ,biology.animal ,Gene expression ,Genetics ,medicine ,Animals ,Humans ,Genetics (clinical) ,030304 developmental biology ,Neurons ,0303 health sciences ,biology ,Brain ,Human brain ,Pan paniscus ,Immunohistochemistry ,medicine.anatomical_structure ,Human evolution ,Evolutionary biology ,Cerebral cortex ,Macaca ,Single-Cell Analysis ,030217 neurology & neurosurgery - Abstract
Identification of gene expression traits unique to the human brain sheds light on the molecular mechanisms underlying human evolution. Here, we searched for uniquely human gene expression traits by analyzing 422 brain samples from humans, chimpanzees, bonobos, and macaques representing 33 anatomical regions, as well as 88,047 cell nuclei composing three of these regions. Among 33 regions, cerebral cortex areas, hypothalamus, and cerebellar gray and white matter evolved rapidly in humans. At the cellular level, astrocytes and oligodendrocyte progenitors displayed more differences in the human evolutionary lineage than the neurons. Comparison of the bulk tissue and single-nuclei sequencing revealed that conventional RNA sequencing did not detect up to two-thirds of cell-type-specific evolutionary differences., Genome Research, 30 (5), ISSN:1088-9051, ISSN:1549-5469
- Published
- 2020
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