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Abstract Chemodynamic therapy represents a novel tumor therapeutic modality via triggering catalytic reactions in tumors to yield highly toxic reactive oxygen species (ROS). Nevertheless, low efficiency catalytic ability, potential systemic toxicity and inefficient tumor targeting, have hindered the efficacy of chemodynamic therapy. Herein, a rationally designed catalytic nanoplatform, composed of folate acid conjugated liposomes loaded with copper peroxide (CP) and chloroquine (CQ; a clinical drug) (denoted as CC@LPF), could power maximal tumor cytotoxicity, mechanistically via maneuvering endogenous and exogenous copper for a highly efficient catalytic reaction. Despite a massive autophagosome accumulation elicited by CP-powered autophagic initiation and CQ-induced autolysosomal blockage, the robust ROS, but not aberrant autophagy, underlies the synergistic tumor inhibition. Otherwise, this combined mode also elicits an early onset, above all, long-term high-level existence of immunogenic cell death markers, associated with ROS and aberrant autophagy -triggered endoplasmic reticulum stress. Besides, CC@LPF, with tumor targeting capability and selective tumor cytotoxicity, could elicit intratumor dendritic cells (mainly attributed to CQ) and tumor infiltrating CD8+ T cells, upon combining with PD-L1 therapeutic antibody, further induce significant anti-tumor effect. Collectively, the rationally designed nanoplatform, CC@LPF, could enhance tumor chemoimmunotherapy via deploying endogenous plus exogenous copper and remodeling tumor microenvironment. Graphical abstract

In this work, two kinds of primary batteries, both of which included a Zn anode, C rod cathode, copper wire and electrolyte composed of Cd2+-contaminated water or soil, were constructed in the first attempt to both remove Cd2+ and generate electricity. Unlike traditional technologies such as electrokinetic remediation with high energy consumption, this technology could realize Cd2+ migration to aggregation and solidification and generate energy at the same time through simultaneous galvanic reactions. The passive surface of Zn and C was proven via electrochemical measurements to be porous to maintain the relatively active galvanic reactions for continuous Cd2+ precipitation. Cd2+ RE (removal efficiency) and electricity generation were investigated under different conditions, based on which two empirical models were established to predict them successfully. In soil, KCl was added to desorb Cd2+ from soil colloids to promote Cd2+ removal. These systems were also proven to remove Cd2+ efficiently when their effects on plants, zebrafish, and the soil bacterial community were tested. LEDs could be lit for days by utilizing the electricity produced herein. This work provides a novel, green, and low-cost route to remediate Cd2+ contamination and generate electricity simultaneously, which is of extensive practical significance in the environmental and energy fields.

Abstract Background Non-small-cell lung cancer (NSCLC) accounts for 80–85% of all lung cancer and is the leading cause of cancer-related deaths globally. Although various treatment strategies have been introduced, the 5-year survival rate of patients with NSCLC is only 20–30%. Thus, it remains necessary to study the pathogenesis of NSCLC and develop new therapeutic drugs. Notably, PYK2 has been implicated in the progression of many tumors, including NSCLC, but its detailed mechanism remains unclear. In this study, we aimed to elucidate the mechanisms through which PYK2 promotes NSCLC progression. Methods The mRNA and protein levels of various molecules were measured using qRT-PCR, western blot (WB), and immunohistochemistry (IHC), respectively. We established stable PYK2 knockdown and overexpression cell lines, and CCK-8, EdU, and clonogenic assays; wound healing, transwell migration, and Matrigel invasion assays; and flow cytometry were employed to assess the phenotypes of tumor cells. Protein interactions were evaluated with co-immunoprecipitation (co-IP), immunofluorescence (IF)-based colocalization, and nucleocytoplasmic separation assays. RNA sequencing was performed to explore the transcriptional regulation mediated by PYK2. Secreted VGF levels were examined using ELISA. Dual-luciferase reporter system was used to detect transcriptional regulation site. PF4618433 (PYK2 inhibitor) and Stattic (STAT3 inhibitor) were used for rescue experiments. A public database was mined to analyze the effect of these molecules on NSCLC prognosis. To investigate the role of PYK2 in vivo, mouse xenograft models of lung carcinoma were established and examined. Results The protein level of PYK2 was higher in human NSCLC tumors than in the adjacent normal tissue, and higher PYK2 expression was associated with poorer prognosis. PYK2 knockdown inhibited the proliferation and motility of tumor cells and caused G1-S arrest and cyclinD1 downregulation in A549 and H460 cells. Meanwhile, PYK2 overexpression had the opposite effect in H1299 cells. The siRNA-induced inhibition of integrins alpha V and beta 1 led to the downregulation of p-PYK2(Tyr402). Activated PYK2 could bind to STAT3 and enhance its phosphorylation at Tyr705, regulating the nuclear accumulation of p-STAT3(Tyr705). This further promoted the expression of VGF, as confirmed by RNA sequencing in a PYK2-overexpressing H1299 cell line and validated by rescue experiments. Two sites in promoter region of VGF gene were confirmed as binding sites of STAT3 by Dual-luciferase assay. Data from the TGCA database showed that VGF was related to the poor prognosis of NSCLC. IHC revealed higher p-PYK2(Tyr402) and VGF expression in lung tumors than in adjacent normal tissues. Moreover, both proteins showed higher levels in advanced TNM stages than earlier ones. A positive linear correlation existed between the IHC score of p-PYK2(Tyr402) and VGF. Knockdown of VGF inhibited tumor progression and reversed the tumor promoting effect of PYK2 overexpression in NSCLC cells. Finally, the mouse model exhibited enhanced tumor growth when PYK2 was overexpressed, while the inhibitors PF4618433 and Stattic could attenuate this effect. Conclusions The Integrin αVβ1-PYK2-STAT3-VGF axis promotes NSCLC development, and the PYK2 inhibitor PF4618433 and STAT3 inhibitor Stattic can reverse the pro-tumorigenic effect of high PYK2 expression in mouse models. Our findings provide insights into NSCLC progression and could guide potential therapeutic strategies against NSCLC with high PYK2 expression levels.

Abstract Rab27A is a small GTPase-mediating exosome secretion, which participates in tumorigenesis of multiple cancer types. Understanding the biological role of Rab27A in non-small cell lung cancer (NSCLC) is of great importance for oncological research and clinical treatment. In this study, we investigate the function and internal mechanism of Rab27A in NSCLC. Results show that Rab27A is overexpressed in NSCLC, and regulates the tumor proliferation, migration, invasion, and cell motility in vitro and in vivo, and is negatively regulated by miR-124. Further research reveals that upregulated Rab27A can induce the production of IFNα in the medium by mediating exosome secretion. Then IFNα activates TYK2/STAT/HSPA5 signaling to promote NSCLC cell proliferation and metastasis. This process can be suppressed by TYK2 inhibitor Cerdulatinib. These results suggest that Rab27A is involved in the pathogenesis of NSCLC by regulating exosome secretion and downstream signaling, and inhibitors targeting this axis may become a promising strategy in future clinical practice.

IntroductionTo analyze the influencing factors for progression from newly diagnosed prediabetes (PreDM) to diabetes within 3 years and establish a prediction model to assess the 3-year risk of developing diabetes in patients with PreDM.MethodsSubjects who were diagnosed with new-onset PreDM at the Physical Examination Center of the First Affiliated Hospital of Soochow University from October 1, 2015 to May 31, 2023 and completed the 3-year follow-up were selected as the study population. Data on gender, age, body mass index (BMI), waist circumference, etc. were collected. After 3 years of follow-up, subjects were divided into a diabetes group and a non-diabetes group. Baseline data between the two groups were compared. A prediction model based on logistic regression was established with nomogram drawn. The calibration was also depicted.ResultsComparison between diabetes group and non-diabetes group: Differences in 24 indicators including gender, age, history of hypertension, fatty liver, BMI, waist circumference, systolic blood pressure, diastolic blood pressure, fasting blood glucose, HbA1c, etc. were statistically significant between the two groups (P0.05). Logistic regression analysis showed that ageing, elevated BMI, male gender, high fasting blood glucose, increased LDL-C, fatty liver, liver dysfunction were risk factors for progression from PreDM to diabetes within 3 years (P

BackgroundExisting studies on the relationship between tea intake and lung diseases have yielded inconsistent results, leading to an ongoing dispute on this issue. The impact of tea consumption on the respiratory system remained elucidating.Materials and methodsWe conducted a two-sample Mendelian randomization (MR) study to evaluate the associations between five distinct tea intake phenotypes and 15 different respiratory outcomes using open Genome-wide association study (GWAS) data. The inverse variance weighted (IVW) was used for preliminary screening and a variety of complementary methods were used as sensitivity analysis to validate the robustness of MR estimates. Pathway enrichment analysis was used to explore possible mechanisms.ResultsIVW found evidence for a causal effect of standard tea intake on an increased risk of lung squamous cell cancer (LSCC) (OR = 1.004; 95% CI = 1.001–1.007; P = 0.00299). No heterogeneity or pleiotropy was detected. After adjustment for potential mediators, including smoking, educational attainment, and time spent watching television, the association was still robust in multivariable MR. KEGG and GO enrichment predicted proliferation and activation of B lymphocytes may play a role in this causal relation. No causalities were observed when evaluating the effect of other kinds of tea intake on various pulmonary diseases.ConclusionOur MR estimates provide causal evidence of the independent effect of standard tea intake (black tea intake) on LSCC, which may be mediated by B lymphocytes. The results implied that the population preferring black tea intake should be wary of a higher risk of LSCC.

The biotoxicity and chemotherapeutic resistance of cisplatin (CDDP) pose a challenge for tumor therapy. Practically, the change in the therapeutic response of tumor from resistance to sensitivity are impressive but challenging. To this end, we propose a strategy of “one stone, three birds” by designing a CuPt nanoalloy to simultaneously eliminate GSH, relieve hypoxia, and promote ROS production for effectively reversing the platinum (IV) (Pt(IV), (c,c,t-[Pt(NH3)2Cl2(OOCCH2CH2COOH)2)) resistance. Notably, the CuPt nanoalloy exhibits ternary catalytic capabilities including mimicking GSH oxidase, catalase and peroxidase. With the subsequent disguise of tumor cell membrane, the CuPt nanoalloy is conferred with homologous targeting ability, making it actively recognize tumor cells and then effectively internalized by tumor cells. Upon entering tumor cell, it gives rise to GSH depletion, hypoxia relief, and oxidative stress enhancement by catalyzing the reaction of GSH and H2O2, which mitigates the vicious milieu and ultimately reinforces the tumor response to Pt(IV) treatment. In vivo results prove that combination therapy of mCuPt and Pt(IV) realizes the most significant suppression on A549 cisplatin-resistant tumor. This study provides a potential strategy to design novel nanozyme for conquering resistant tumor.

In view of the difficult situation of fire prevention, control, disaster resistance and relief in complex ventilation networks, the laws of air flow disturbance, heat transfer and smoke flow diffusion in air flow field, temperature field and smoke flow concentration field are analyzed. According to the evolution law of smoke flow at different fire source locations, an emergency linkage regulation scheme of fire air and smoke flow that can simultaneously meet the requirements of smoke exhaust air volume and stope air volume is proposed. Taking Zhuanlongwan Coal Mine as an application case, the fire smoke emergency linkage control system of Zhuanlongwan Coal Mine was established. In order to analyze the effect of air control and smoke exhaust, combined with the actual tunnel and ventilation parameters, a three-dimensional visualization model of its complex ventilation network was built based on VENTSIM software. The VentFire module is used to simulate the wind and smoke spreading effect of the fire scene in different fire source locations. The smoke exhaust effect and air volume distribution before and after the emergency control scheme is adopted are analyzed. The results show that when a fire occurs in different locations, the emergency smoke exhaust scheme should be started in time. On the basis of meeting the smoke exhaust air volume, the air volume of other key locations can still ensure more than 90% of the normal ventilation period, which can meet the disaster control and relief needs during the disaster of Zhuanlongwan Coal Mine.

BackgroundNon-suicidal self-injury (NSSI) is a common feature among adolescents with mood disorders. Although childhood maltreatment has shown to be associated with non-suicidal self-injury (NSSI), previous studies have yielded mixed results in terms of different subtypes of childhood maltreatment and only few studies have investigated the effects of gender. The present cross-sectional study investigated effects of different types of childhood maltreatment on NSSI, as well as the role of gender in these effects.MethodsIn this cross-sectional study, a total of 142 Chinese adolescent inpatients with mood disorders (37 males and 105 females) were consecutively recruited within a psychiatric hospital. Demographic and clinical characteristics were collected. Participants were administered the Childhood Trauma Questionnaire (CTQ), the Functional Assessment of Self-Mutilation (FASM).Results76.8% of the sample reported engaging NSSI in the previous 12 months. Female participants were more likely to engage in NSSI than males (p

The development of highly efficient magnetic resonance imaging (MRI) contrast agent contributes to the early and accurate diagnosis of cancer. Herein, we developed a novel iron oxide and gadolinium oxide (IO/GdO) nanocomposite as T1-T2 dual modal MRI contrast agent through hydrothermal synthesis method. Subsequently, tumor-targeting ligand (PDGFB-PEG) was further conjugated to the surface of IO/GdO nanocomposite (PDGFB-pIO/GdO) to fabricate tumor-targeting MRI probe for accurate diagnosis of orthotopic prostatic cancer. IO/GdO nanocomposite exhibited obvious mesoporous structure, which would promote the contact between water molecules and Gd or Fe centers and enhance the T1 relaxation rate (r1) and T2 relaxation rate (r2) of IO/GdO composite. In addition, the r1 and r2 values of IO/GdO composite were up to be 11.6 mM−1s−1 and 102.7 mM−1s−1, respectively, and the r2/r1 ratio of IO/GdO nanocomposite was calculated to be 8.85, demonstrating a great potential as dual-mode MRI contrast agent. Further study indicated that PDGFB-pIO/GdO exhibited an excellent biocompatibility. Besides, PDGFB-pIO/GdO presented a higher accumulation in tumor than untargeted pIO/GdO, confirming its excellent in vivo tumor-targeting ability. Lastly, in orthotopic prostatic cancer model, the systemic delivery of PDGFB-pIO/GdO significantly enhanced T1- and T2-weighted signal of orthotopic prostatic cancer, achieving high quality of MR images and realizing accurate diagnosis of orthotopic prostatic cancer. Collectively, we proposed a rational designed nanoplatform, PDGF-B conjugating mesoporous IO/GdO nanocomposites, suggesting potential for diagnostic identification of prostate cancer via T1-T2 dual-modal MRI contrast enhancement.

Abstract The cell membrane is widely considered as a promising delivery nanocarrier due to its excellent properties. In this study, self-assembled Pseudomonas geniculate cell membranes were prepared with high yield as drug nanocarriers, and named BMMPs. BMMPs showed excellent biosafety, and could be more efficiently internalized by cancer cells than traditional red cell membrane nanocarriers, indicating that BMMPs could deliver more drug into cancer cells. Subsequently, the BMMPs were coated with nanoselenium (Se), and subsequently loaded with Mn2+ ions and doxorubicin (DOX) to fabricate a functional nanoplatform (BMMP-Mn2+/Se/DOX). Notably, in this nanoplatform, Se nanoparticles activated superoxide dismutase-1 (SOD-1) expression and subsequently up-regulated downstream H2O2 levels. Next, the released Mn2+ ions catalyzed H2O2 to highly toxic hydroxyl radicals (·OH), inducing mitochondrial damage. In addition, the BMMP-Mn2+/Se nanoplatform inhibited glutathione peroxidase 4 (GPX4) expression and further accelerated intracellular reactive oxygen species (ROS) generation. Notably, the BMMP-Mn2+/Se/DOX nanoplatform exhibited increased effectiveness in inducing cancer cell death through mitochondrial and nuclear targeting dual-mode therapeutic pathways and showed negligible toxicity to normal organs. Therefore, this nanoplatform may represent a promising drug delivery system for achieving a safe, effective, and accurate cancer therapeutic plan.

The refuge chamber provides a safe space where people can stay safe when they are trapped by an accident in a coal mine. The refuge chamber includes several systems such as oxygen supply, air purification, cooling, and dehumidifying. The oxygen supply system is the core of the refuge chamber because it is a closed environment. The oxygen supply time is 96 h according to the relevant standards in America and China. If people stay in the refuge chamber for more than 96 h, the oxygen supply may not be enough, and they can be in danger. It is necessary to efficiently use oxygen and extend the oxygen supply time. Based on the experimental platform of the refuge chamber, this paper conducts an in-depth study on the automatic control of the oxygen supply system. The oxygen supply system and the harmful gas removal system can be automatically and efficiently controlled by the programmable logic controller (PLC) according to the personnel number and the oxygen concentration. The experiment has proved that the system is good and reliable.

The indicative ability of a gas indicator for the spontaneous combustion of coal is affected by the secondary oxidation of oxidized coal, from old goafs, entering a new goaf through air leakages. This phenomenon can affect the accuracy of early warning systems regarding the spontaneous combustion of coal in a goaf. In this research, three kinds of coal were selected to carry out a spontaneous combustion simulation experiment in which a temperature-programmed experimental device was used to analyze the behavior of the index gas towards raw coal and oxidized coal, for which the latter was oxidized at 70 °C, 90 °C, 130 °C, and 150 °C. The results show that the chain alkane ratio in the secondary oxidation process and the trends of oxygen, CO, and C2H4 concentrations are the same as those in the primary oxidation process. On the other hand, the temperature at which C2H4 initially appears, during secondary oxidation, is lower than in primary oxidation. The CO produced in the early stage of secondary oxidation is greater than the CO produced, at the same temperature, in primary oxidation. In this regard, the usage of C2H4 concentration as an indicator with which to judge the occurrence of the spontaneous combustion of coal would allow for an earlier response. In the secondary oxidation process, the temperature of the extreme value of the alkene ratio appears higher than in primary oxidation. The presence of a higher pre-oxidation temperature and a higher proportion of secondary oxidation gas will affect an indicator’s judgement when the primary oxidation enters the severe oxidation stage. The gas produced by secondary oxidation will affect the early warning of the spontaneous combustion of coal in the coal mine goaf, which should be considered in the establishment of an early warning system.

Abstract Background Post-partum depression (PPD) is a growing mental health concern worldwide. There is little evidence in the Chinese context of the relationship between paternal PPD and maternal PPD. Given the growing global concerns this relationship requires further exploration. Methods A survey was conducted with 950 total couples from March 2017 to December 2018. The study was conducted using a standardized questionnaire that included basic demographic information, information on the relationship between the mother-in-law and daughter-in-law, marital satisfaction (both maternal and paternal), and PPD symptoms. Structural Equation Modelling (SEM) analysis was used to explore the underlying mechanism for PPD symptoms in mothers and fathers. Results In 4.4% of the couples both the wife and the husband showed depressive symptoms. Maternal marital satisfaction showed a significant mediating effect on paternal PPD (B = -0.114, p

During coal mine production, the unbalanced emission of stope gas and other toxic and harmful gases can easily lead to major disasters and accidents. In view of the structural characteristics of coal mine excavation ventilation system, a three-dimensional ventilation network (VN) experimental model with an independent stope is constructed and regulated fan frequency conversion (FC) is developed. The concept of influenced degree is proposed, and it is quantified for the stope branch in a complex VN model. An experiment is conducted on FC and air-regulating dilution of quantitative gas release. It is concluded that the gas concentration in the stope of a working face has a power function relationship with the working frequency of the fan. According to mining replacement characteristics, five wind regulation rules for supply and demand matching (SDM) were set during a working day, and the feasibility of dynamic treatment and control adjustment of the gas emission at the mining surface was verified experimentally. Finally, the dynamic SDM control model of stope ventilation has been applied in a mine operated by the Yangquan coal mine. The results show that the stope gas disaster risk can be dispensed quickly and effectively when the emission is unbalanced.

The modification of biochar is essential for the development of multifunctional biochar materials with enhanced remediation effects on contaminated water. In this work, a biochar-based microcatalyst with sunlight sensitivity was synthesized by a creative modification method that involved the rapid fabrication of MnO2 microspheres by high-energy electron beam (HEEB) irradiation, and loading them into corn straw-derived honeycomb-like KOH-modified biochar (MBC) to obtain a sunlight-sensitive microcatalyst (SSM). The honeycomb-like structure of MBC facilitated the improvement in MnO2 dispersion and photocatalytic property through confinement effect. The effects of photocatalyst dosage, initial chlortetracycline (CTC) concentration, solution pH, temperature and coexisting ions on the photocatalytic performance of SSM were systemically investigated. The results indicated that SSM could efficiently degrade CTC in water and swine urine under sunlight, and exhibited high stability against coexistence of urea, Cl− and SO42−. Moreover, SSM showed good reusability in regeneration studies. This work provides a novel method for degrading CTC with potential application prospect.

To solve the existing problems of imperfect sealing and the inaccurate measurement of gas pressure in traditional sealing, the present study builds a new model of capsule-slime sealing device based on wireless pressure gauge. The new sealing device is mainly composed of two sets of capsules, a capsule connecting piece and a slime part, measures the pressure through the wireless communication technology, and seals through chemical reaction in the capsule, which generates gas to expand the capsule and extrude the pre-stored slime. Two methods of extruding pre-stored slime are proposed: in the first method, transverse force is generated by the expansion of the capsules at both ends, pushing the capsules toward the middle through the chute and squeezing the pre-stored slime out; in the second, high-pressure gas generated in the capsules is led into the expansion tube, which is inserted into the storage tube, squeezing it and letting the slime out to complete the sealing process. Then the research studies the effect of sealing under the condition of drilling with the structure of slime storage tube. The results indicate that the maximum standing time of slime wrapped in metal net is 2 h, instead of the expected 10 d, failing to meet the sealing requirements. When the slime viscosity is 1200–3000 mPa·s in the structure of slime storage tube, the best viscosity of slime is achieved and can simulate sealing the drilling hole (5–4, 4–2.36, 2.36–1.18, 1.18–0.15 mm in diameter), as well as the gap caused by mixed sand accumulation. Keywords: Wireless pressure gauge, Capsule-slime, Hole sealing device, Simulated sealing

This study assessed the impact of reducing dissolved oxygen (DO) content on the quality of UHT milk using a flash deoxygenation treatment. Flash deoxygenation was designed based on preheated milk reaching boiling early under low-pressure conditions to remove DO from the milk. Two parameters were designed for flash deoxygenation: preheating temperature 65 °C, −0.08 Mpa, and 70 °C, −0.06 Mpa. The flash conditions were applied to two UHT sterilization conditions (135 °C for 10 s and 145 °C for 5 s). After deoxygenation, the total oxidation (TOTOX) value of UHT milk was reduced by 1.4~1.71, and the protein carbonyl (PC) value was reduced by 1.15~1.52 nmol/mg of protein. The maximum inhibition rates of furusine and 5-HMF were 33.23 ± 1.72% and 25.43 ± 3.14%, respectively. The particle size was reduced by 0.141~0.178 μm. The ketones and stale aldehydes causing oxidized taste in the UHT milk were significantly reduced. This study showed that the oxidation and Maillard reactions of UHT milk were significantly inhibited, stability was improved, and the content of undesirable volatile flavor substances was reduced after flash deoxygenation. Therefore, reducing DO content was beneficial to improving the quality of UHT milk.

Over the past few decades, enzyme-based green and sustainable chemistry has attracted extensive research attention, which provides a promising alternative to the conventional treatment methods of recalcitrant micropollutants. However, enzyme denaturation and stability loss remain critical challenges for its potential applications in industrial wastewater treatment. In this study, laccase from Trametes versicolor (laccase T.) was cross-linked immobilized by ultraporous alumina (UPA) for the sustainable biodegradation of Remazol Brilliant Blue R (RBBR). Through sequential use of an aminosilane coupling agent (3-aminopropyl)triethoxysilane (APTES) and bifunctional cross-linker glutaraldehyde (GA), the synthesized biocatalysts showed better immobilization performances (about 4-fold to physical adsorption). The GA concentration considerably affected the laccase T. cross-linking degree, while the GA post-treatment protocol showed the highest laccase T. immobilization yield with lower activity recovery. Moreover, the biocatalyst stabilities including pH stability, thermal stability, storage stability, and reusability were also studied. Tolerance to broader pH and temperature ranges, better storage stability, good reusability of laccase T. cross-linked UPA(γ) biocatalysts, and their continuous RBRR biodegradation efficiency highlight the potentials of enzyme-based inorganic materials in industrial wastewater treatment, which can broaden our understanding of their practical applications in environmental fields.

Every year, a large amount of tobacco waste liquid (TWL) is discharged into the environment, resulting in serious pollution for the environment. In this work, a TWL-based particle (OACT) was fabricated by CaO, attapulgite (ATP), and TWL, and, then, OACT was coated by amino silicon oil (ASO) to form OACT@ASO. Therein, OACT@ASO had high controlled-release ability for fulvic acid (FA), because of the nanonetworks structure for ATP and the high content of FA in TWL. The release ratio (RR) of FA from OACT@ASO reached 94% at 75 h in deionized water, and 23% at 32 d in silica sand. Furthermore, the release mechanism of FA from OACT@ASO was consistent with the First-order law. Additionally, OACT@ASO also possessed high immobilization capacity for Cu(II), Cd(II), and Pb(II) (CCP) in soil. Notably, a pot experiment indicated that OACT@ASO could facilitate the growth of pakchoi seedlings and decrease the absorption of CCP by pakchoi seedlings. Thus, this study provides a new kind of organic fertilizer which could not only release FA, but also immobilize CCP in soil.

ABSTRACTIn this study, a potential novel cellulolytic bacteriumArthrobacter sp. HPG166 was isolated from the hindgut of root-feeding larvaeHolotrichia parallela. Optimization of fermentation factors for endoglucanase production byArthrobacter sp. HPG166 was carried out via response surface methodology. Sodium carboxymethylcellulose 1.19% (w/v) and beef extract 0.35% (w/v) were the ideal combination of carbon and nitrogen sources for enzyme production; the optimum temperature and pH for cellulase production were 34°C and pH 8.0 respectively. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.411 U mL-1 was obtained. The crude endoglucanase was thermotolerant as it retained 50.31% of its activity after incubation at 70°C for an hour. Metal profile of the enzyme indicated that Mg2+ and Na+ were strong stimulators while Mn2+ and Co+ drastically inhibited its activity. Due to its particular characteristics, this enzyme could have potential for industrial applications.

Generally, crop straw degrades slowly in soil, which is unfavorable for tillage and next crop growth. Thus, it is important to develop a promising technology to boost degradation of straw. Herein, a nanobiosystem has been developed by loading bacterial mixture in nanostructured attapulgite (ATP) and using it as a straw returning agent (SRA). Therein, ATP could effectively anchor bacteria to the surface of straw and greatly facilitate the adhesion and growth of bacteria. Consequently, this technology could effectively accelerate the degradation and transformation of straw into nutrients, including nitrogen (N), phosphorus (P), potassium (K), and organic matters (OM). Pot and field tests indicated that SRA displayed significant positive effects on the growth of the next crop. Importantly, SRA could effectively decrease greenhouse gas emissions from farmland, which is beneficial for the environment. Therefore, this work provides a facile and promising method to facilitate the degradation of straw, which might have a potential application value.

Raman spectroscopy and nonisothermal thermogravimetric analysis (TGA) measurements have been reported for different rank coals (lignite, bituminous coal, and anthracite) and the relationship between the measurements was examined. It was found that the Raman spectra parameters can be used to characterize structure changes in the different rank coals, such as the band area ratios based on the curve-fitted results. Higher ranked coal was found to have higher values of IGR/IAll and IG+GR/IAll but lower values of ID/I(G+GR), IDL/I(G+GR), IS+SL/I(G+GR), and I(GL+GL')/I(G+GR). The oxidation properties of the coal samples were characterized by the reactivity indexes Tig, T20%, and Tmax from TGA data which were found to correlate well with the band area ratios of IGR/IAll, IG+GR/IAll, and IS+SL/I(G+GR). Based on these correlations, the Raman band area ratios were found to correlate with the oxidation activity of coal providing additional structural information which can be used to understand the changes in the TGA measurements.

Background: Non-suicidal self-injury (NSSI) is a common feature among adolescents with mood disorders. Although childhood maltreatment has shown to be associated with non-suicidal self-injury (NSSI), previous studies have yielded mixed results in terms of different subtypes of childhood maltreatment and only few studies have investigated the effects of gender. The present cross-sectional study investigated effects of different types of childhood maltreatment on NSSI, as well as the role of gender in these effects. Methods: In this cross-sectional study, a total of 142 Chinese adolescent inpatients with mood disorders (37 males and 105 females) were consecutively recruited within a psychiatric hospital. Demographic and clinical characteristics were collected. Participants were administered the Childhood Trauma Questionnaire (CTQ), the Functional Assessment of Self-Mutilation (FASM). Results: 76.8% of the sample reported engaging NSSI in the previous 12 months. Female participants were more likely to engage in NSSI than males (p < 0.001). Participants in the NSSI group reported significantly more experiences of emotional abuse (p < 0.001) and emotional neglect (p = 0.005). With regards to gender differences, female participants who have experienced emotional abuse were more likely to engage in NSSI (p = 0.03). Conclusion: As a whole, NSSI represents a frequent phenomenon among adolescent clinical populations and females were more likely to engage in NSSI than males. NSSI was significantly related to experiences of childhood maltreatment and specifically related to emotional abuse and emotional neglect over and above other types of childhood maltreatment. Females were more sensitive to emotional abuse than males. Our study highlights the importance of screening for subtypes of childhood maltreatment as well as considering the effects of gender. [ABSTRACT FROM AUTHOR]