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5 results on '"Zheng-kun Tang"'

1. Efficacy and safety of pelvic floor magnetic stimulation combined with mirabegron in female patients with refractory overactive bladder: a prospective study

Author

Ping Liang, Qing-lai Tang, Tao Lin, Zheng-kun Tang, Fa-de Liu, Xing-zhu Zhou, and Rong-zhen Tao

Subjects
refractory overactive bladder, pelvic floor magnetic stimulation, mirabegron, combination therapy, incontinence, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

ObjectiveTo observe the efficacy and safety of pelvic floor magnetic stimulation (PFMS) combined with mirabegron in female patients with refractory overactive bladder (OAB) symptoms.Patients and methodsA total of 160 female patients with refractory OAB symptoms were prospectively randomized into two groups. Eighty cases in the combination group accepted PFMS and mirabegron therapy and 80 cases as control only accepted mirabegron therapy (The clinical trial registry number: ChiCTR2200070171). The lower urinary tract symptoms, OAB questionnaire (OAB-q) health-related quality of life (HRQol), symptom bother score and OABSS between two groups were compared at the 1st, 2nd and 4th week ends.ResultsAll of 160 patients were randomly assigned to two groups, of which 80 patients were included in the combination group and 80 in the mirabegron group. The incidences of LUTS, including urgency, frequent urination, and incontinence episodes, in the 2nd week and the 4th week after combination treatment were significantly lower than those in the mirabegron group (p 0.05). With respect to secondary variables, the OAB-q HRQol score in the combination group was statistically superior in comparison with that in the mirabegron group between the 2nd week and the 4th week (p

Published
2024
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2. Erratum to: Characterization of three pathway-specific regulators for high production of monensin in Streptomyces cinnamonensis

Author

Xiao-Mei Li, Chun-Yan Lin, Zheng-Kun Tang, Guang-Rong Zhao, Ai-Ping Pang, Yue Zhang, Jianjun Qiao, and Jie Zhang

Subjects
biology, Monensin, General Medicine, biology.organism_classification, Applied Microbiology and Biotechnology, Streptomyces, Complementation, Reverse transcription polymerase chain reaction, Metabolic pathway, chemistry.chemical_compound, Biochemistry, chemistry, Transcription (biology), Gene cluster, Gene, Biotechnology
Abstract

Monensin, a polyether ionophore antibiotic, is produced by Streptomyces cinnamonensis and worldwide used as a coccidiostat and growth-promoting agent in the field of animal feeding. The monensin biosynthetic gene cluster (mon) has been reported. In this study, the potential functions of three putatively pathway-specific regulators (MonH, MonRI, and MonRII) were clarified. The results from gene inactivation, complementation, and overexpression showed that MonH, MonRI, and MonRII positively regulate monensin production. Both MonH and MonRI are essential for monensin biosynthesis, while MonRII is non-essential and could be completely replaced by additional expression of monRI. Transcriptional analysis of the mon cluster by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and electrophoresis mobility shift assays (EMSAs) revealed a co-regulatory cascade process. MonH upregulates the transcription of monRII, and MonRII in turn enhances the transcription of monRI. MonRII is an autorepressor, while MonRI is an autoactivator. MonH activates the transcription of monCII-monE, and upregulates the transcription of monT that is repressed by MonRII. monAX and monD are activated by MonRI, and upregulated by MonRII. Co-regulation of those post-polyketide synthase (post-PKS) genes by MonH, MonRI, and MonRII would contribute to high production of monensin. These results shed new light on the transcriptional regulatory cascades of antibiotic biosynthesis in Streptomyces.

Published
2017

3. Characterization of three pathway-specific regulators for high production of monensin in Streptomyces cinnamonensis

Author

Zheng-Kun Tang, Xiao-Mei Li, Ai-Ping Pang, Chun-Yan Lin, Yue Zhang, Jie Zhang, Jianjun Qiao, and Guang-Rong Zhao

Subjects
0301 basic medicine, Transcription, Genetic, Genetic Complementation Test, 030106 microbiology, Gene Expression Regulation, Bacterial, General Medicine, Applied Microbiology and Biotechnology, Streptomyces, Anti-Bacterial Agents, 03 medical and health sciences, 030104 developmental biology, Multigene Family, Gene Silencing, Cloning, Molecular, Monensin, Gene Deletion, Metabolic Networks and Pathways, Biotechnology
Abstract

Monensin, a polyether ionophore antibiotic, is produced by Streptomyces cinnamonensis and worldwide used as a coccidiostat and growth-promoting agent in the field of animal feeding. The monensin biosynthetic gene cluster (mon) has been reported. In this study, the potential functions of three putatively pathway-specific regulators (MonH, MonRI, and MonRII) were clarified. The results from gene inactivation, complementation, and overexpression showed that MonH, MonRI, and MonRII positively regulate monensin production. Both MonH and MonRI are essential for monensin biosynthesis, while MonRII is non-essential and could be completely replaced by additional expression of monRI. Transcriptional analysis of the mon cluster by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and electrophoresis mobility shift assays (EMSAs) revealed a co-regulatory cascade process. MonH upregulates the transcription of monRII, and MonRII in turn enhances the transcription of monRI. MonRII is an autorepressor, while MonRI is an autoactivator. MonH activates the transcription of monCII-monE, and upregulates the transcription of monT that is repressed by MonRII. monAX and monD are activated by MonRI, and upregulated by MonRII. Co-regulation of those post-polyketide synthase (post-PKS) genes by MonH, MonRI, and MonRII would contribute to high production of monensin. These results shed new light on the transcriptional regulatory cascades of antibiotic biosynthesis in Streptomyces.

Published
2017

5. DasR positively controls monensin production at two-level regulation in Streptomyces cinnamonensis

Author

Xiao-Mei Li, Zheng-Kun Tang, Jianjun Qiao, Guang-Rong Zhao, Yue Zhang, and Chun-Yan Lin

Subjects
0301 basic medicine, animal structures, 030106 microbiology, Bioengineering, Applied Microbiology and Biotechnology, Streptomyces, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Bioreactors, Bacterial Proteins, Gene cluster, Genes, Regulator, Animals, Electrophoretic mobility shift assay, Monensin, Gene, Regulator gene, biology, Streptomyces coelicolor, Gene Expression Regulation, Bacterial, biology.organism_classification, Anti-Bacterial Agents, 030104 developmental biology, chemistry, Biochemistry, Multigene Family, Saccharopolyspora erythraea, Cattle, Gene Deletion, Biotechnology, Transcription Factors
Abstract

The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S. cinnamonensis. Forward and reverse genetics by overexpression and antisense RNA silence of dasR revealed that DasR positively controls monensin production under nutrient-rich condition. Electrophoresis mobility shift assay (EMSA) showed that DasR protein specifically binds to the promoter regions of both pathway-specific regulatory gene monRII and biosynthetic genes monAIX, monE and monT. Semi-quantitative RT-PCR further confirmed that DasR upregulates the transcriptional levels of these genes during monensin fermentation. Subsequently, co-overexpressed dasR with pathway-specific regulatory genes monRI, monRII or monH greatly improved monensin production.

Published
2016

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