Search

Your search keyword '"Zheng, Zi-Zheng"' showing total 33 results
Start Over Author "Zheng, Zi-Zheng"
33 results on '"Zheng, Zi-Zheng"'

10. Profile of clinical characteristics and serologic markers of sporadic hepatitis E in a community cohort study

Author

Tang, Zi-Min, primary, Wen, Gui-Ping, additional, Ying, Dong, additional, Wang, Si-Ling, additional, Liu, Chang, additional, Tian, Wei-Kun, additional, Wang, Ying-Bin, additional, Fang, Mu-Jin, additional, Zhou, Yu-Lin, additional, Ge, Yun-Sheng, additional, Wu, Ting, additional, Zhang, Jun, additional, Huang, Shou-Jie, additional, Zheng, Zi-Zheng, additional, and Xia, Ning-Shao, additional

Published
2022
Full Text
View/download PDF

14. Molecular Evolution of Attachment Glycoprotein (G) and Fusion Protein (F) Genes of Respiratory Syncytial Virus ON1 and BA9 Strains in Xiamen, China

Author

Sun, Yong-Peng, primary, Lei, Si-Yu, additional, Wang, Ying-Bin, additional, Wang, Yi-Zhen, additional, Qiang, Hong-Sheng, additional, Yin, Yi-Fan, additional, Jiang, Ze-Min, additional, Zhu, Min, additional, Chen, Xiao-Li, additional, Ye, Hui-Ming, additional, Zheng, Zi-Zheng, additional, and Xia, Ning-Shao, additional

Published
2022
Full Text
View/download PDF

15. Neutralizing breadth of antibodies targeting diverse conserved epitopes between SARS-CoV and SARS-CoV-2

Author

Xiong, Hua-Long, primary, Sun, Hui, additional, Wang, Si-Ling, additional, Yuan, Lunzhi, additional, Liu, Liqin, additional, Zhu, Yuhe, additional, Zhang, Jinlei, additional, Huang, Yang, additional, Qi, Ruoyao, additional, Jiang, Yao, additional, Ma, Jian, additional, Zhou, Min, additional, Ma, Yue, additional, Fu, Rao, additional, Yan, Siping, additional, Yue, Mingxi, additional, Wu, Yangtao, additional, Wei, Min, additional, Wang, Yi-Zhen, additional, Li, Tingting, additional, Wang, Yingbin, additional, Zheng, Zi-Zheng, additional, Yu, Hai, additional, Cheng, Tong, additional, Li, Shaowei, additional, Yuan, Quan, additional, Zhang, Jun, additional, Guan, Yi, additional, Zheng, Qingbing, additional, Zhang, Tianying, additional, and Xia, Ningshao, additional

Published
2022
Full Text
View/download PDF

18. Lot-to-lot consistency study of an Escherichia coli-produced bivalent human papillomavirus vaccine in adult women: a randomized trial

Author

Su, Ying-Ying, primary, Lin, Bi-Zhen, additional, Zhao, Hui, additional, Li, Juan, additional, Lin, Zhi-Jie, additional, Qiao, You-Lin, additional, Wei, Li-Hui, additional, Hu, Yue-Mei, additional, Li, Rong-Cheng, additional, Zhuang, Si-Jie, additional, Sun, Guang, additional, Zheng, Zi-Zheng, additional, Huang, Shou-Jie, additional, Wu, Ting, additional, Zhang, Jun, additional, Pan, Hui-Rong, additional, and Li, Chang-Gui, additional

Published
2019
Full Text
View/download PDF

19. The Optimal Concentration of Formaldehyde is Key to Stabilizing the Pre-Fusion Conformation of Respiratory Syncytial Virus Fusion Protein

Author

Zhang, Wei, primary, Zhang, Lu-Jing, additional, Zhan, Lu-Ting, additional, Zhao, Min, additional, Wu, Guang-Hua, additional, Si, Jun-Yu, additional, Chen, Li, additional, Lin, Xue, additional, Sun, Yong-Peng, additional, Lin, Min, additional, Yu, Chao, additional, Fang, Mu-Jin, additional, Wang, Ying-Bin, additional, Zheng, Zi-Zheng, additional, and Xia, Ning-Shao, additional

Published
2019
Full Text
View/download PDF

22. Structural basis of respiratory syncytial virus subtype-dependent neutralization by an antibody targeting the fusion glycoprotein

Author

Tian, Daiyin, primary, Battles, Michael B., additional, Moin, Syed M., additional, Chen, Man, additional, Modjarrad, Kayvon, additional, Kumar, Azad, additional, Kanekiyo, Masaru, additional, Graepel, Kevin W., additional, Taher, Noor M., additional, Hotard, Anne L., additional, Moore, Martin L., additional, Zhao, Min, additional, Zheng, Zi-Zheng, additional, Xia, Ning-Shao, additional, McLellan, Jason S., additional, and Graham, Barney S., additional

Published
2017
Full Text
View/download PDF

23. Discovery of a Prefusion Respiratory Syncytial Virus F-Specific Monoclonal Antibody That Provides Greater In Vivo Protection than the Murine Precursor of Palivizumab

Author

Zhao, Min, primary, Zheng, Zi-Zheng, additional, Chen, Man, additional, Modjarrad, Kayvon, additional, Zhang, Wei, additional, Zhan, Lu-Ting, additional, Cao, Jian-Li, additional, Sun, Yong-Peng, additional, McLellan, Jason S., additional, Graham, Barney S., additional, and Xia, Ning-Shao, additional

Published
2017
Full Text
View/download PDF

29. A Valuable Antigen Detection Method for Diagnosis of Acute Hepatitis E

Author

Wen, Gui-Ping, Tang, Zi-Min, Yang, Fan, Zhang, Ke, Ji, Wen-Fang, Cai, Wei, Huang, Shou-Jie, Wu, Ting, Zhang, Jun, Zheng, Zi-Zheng, and Xia, Ning-Shao

Abstract

ABSTRACTHepatitis E virus (HEV) is a serious public health problem. The commonly used tests that are specific for current HEV infection diagnosis include the detection of anti-HEV IgM and HEV RNA. Here, we report an improved enzyme-linked immunosorbent assay (ELISA) method for HEV antigen detection with a linear range equivalent to 6.3 × 103to 9.2 × 105RNA copies per ml. The monoclonal antibody (MAb) 12F12, a high-ability MAb that binds HEV virus, was selected as the capture antibody from a panel of 95 MAbs. The positive period of HEV antigenemia in infected monkeys using this test was, on average, 3 weeks longer than previously reported and covered the majority of the acute phase. The positive detection rates of IgM, RNA, and new antigen from the first serum samples collected from 16 confirmed acute hepatitis E patients were 81% (13/16), 81% (13/16), and 100% (16/16), respectively. In three patients, the initial serum specimens that tested negative for IgM, despite the presence of symptoms of acute hepatitis and elevated alanine aminotransferase (ALT) levels, were positive for HEV antigen and HEV RNA. In contrast, the serum samples of the three RNA-negative patients were antigen positive (and IgM positive), possibly due to the degradation of HEV nucleic acids. Our results suggest that this new antigen detection method has acceptable concordance with RNA detection and could serve as an important tool for diagnosing acute hepatitis E.

Published
2014
Full Text
View/download PDF

30. [Localization of functional domains of HEV ORF1 in cells].

Author

Huang H, Zheng ZZ, Zhao M, Li JX, Lai WS, Miao J, Zhang J, and Xia NS

Subjects
Blotting, Western, Cells, Cultured, Humans, Viral Proteins genetics, Viral Proteins physiology, Hepatitis E virus genetics, Open Reading Frames
Abstract

To investigate the expression and localization of various functional domains of ORF1 polyprotein and ORF3 protein of hepatitis E virus in host cells, the coding sequences of the various functional domains (RdRp, HEL, MET, PLP, X) of ORF1 were separately cloned into pcDNA3. 1-GFP vectors for constructing the recombinant plasmids which were verified by enzyme digestion and sequencing. The exact expression of the fusion proteins were detected by Western Blot, and the distribution and localization were observed by the laser scanning confocal microscope(LSCM). In huh7 cells, GFP-RdRp proteins were found mainly in the nuclei, GFP-HEL proteins were distributed vesicularly around the nucleus, GFP-MET proteins were distributed granularly both in the nuclei and the cytoplasm, GFP-PLP proteins had polar distribution around the nucleus, and unknown GFP-X proteins were distributed uniformly both in the nuclei and the cytoplasm. Different localization of these proteins verified the previous data obtained from in vitro studies, providing a support for further research on the biological functions of various proteins coded by HEV genome.

Published
2011

31. Role of heat-shock protein 90 in hepatitis E virus capsid trafficking.

Author

Zheng ZZ, Miao J, Zhao M, Tang M, Yeo AE, Yu H, Zhang J, and Xia NS

Subjects
Antiviral Agents pharmacology, Benzoquinones pharmacology, Cell Line, Tumor, HSP90 Heat-Shock Proteins antagonists & inhibitors, Hepatitis E virus drug effects, Humans, Lactams, Macrocyclic pharmacology, Virus Replication drug effects, Virus Replication physiology, Capsid Proteins physiology, HSP90 Heat-Shock Proteins metabolism, Hepatitis E virus physiology, Protein Transport physiology
Abstract

p239 is a virus-like particle constituted from hepatitis E virus (HEV) recombinant proteins. It can be used as a surrogate for HEV and as an investigative tool to study cellular interactions because of its ability to adsorb to and penetrate HepG2 cellular membranes. Our objective was to use p239 to define the role of HEV capsid proteins during the early stages of infection. Pull-down and MALDI-TOF MS experiments identified three host-cell proteins, Grp 78/Bip, alpha-tubulin and heat-shock protein 90 (HSP90), and the latter was investigated further. Antibodies to p239 alone or HSP90 alone could detect p239 or HSP90, suggesting the formation of a complex between p239 and HSP90. In the HepG2 cell, geldanamycin (GA), an HSP90-specific inhibitor, blocked intracellular transportation of p239, but had no effect on the binding and cellular entry of p239, suggesting that HSP90 was important for HEV capsid intracellular transportation. RT-PCR results showed that the efficiency of wild-type HEV infection was inhibited significantly by GA treatment, suggesting the importance of HSP90 in virus infectivity. It was concluded that HSP90 plays a crucial role in the intracellular transportation of viral capsids in the early stage of HEV infection.

Published
2010
Full Text
View/download PDF

32. [HEV capsid protein interacts with CYP 2A6 and decreases its coumarin 7-hydroxylation activity].

Author

Tang M, Zheng ZZ, Sun YY, He SZ, Zhao M, Huang H, Miao J, Zhang J, and Xia NS

Subjects
Aryl Hydrocarbon Hydroxylases genetics, Capsid Proteins genetics, Cell Line, Tumor, Coumarins metabolism, Cytochrome P-450 CYP2A6, Humans, Imidazoles metabolism, Immunoprecipitation, Protein Binding, Recombinant Proteins genetics, Reverse Transcriptase Polymerase Chain Reaction, Aryl Hydrocarbon Hydroxylases metabolism, Capsid Proteins metabolism, Hepatitis E virus metabolism, Recombinant Proteins metabolism
Abstract

E2 is a recombinant hepatitis E virus capsid protein including its main antigenic determinants but lacking of the particle assembling domain. P239 was the C-terminal extending protein of E2 and could self-assemble to form virus like particles, which might serve as mimicry of virions both structurally and antigenically. We previously used yeast two-hybrid system to screen proteins interacting with E2 based on a human hepatocyte cDNA library. One candidate was identified as the segment (aa388-437) of cytochrome P450 2A6 protein, which is predominantly expressed in liver and important for metabolization. Some studies have demonstrated that hepatitis virus infection may altered cell metabolic clearance of coumrarin which were rapidly matebolised by CYP2A6. In this research, we demonstrated that the protein interaction between HEV capsid proteins and CYP2A6 by pull-down and co-immunoprecipitation. It was also found that their interaction could decrease the CYP2A6 catalytic activity when p239 was incubated within the CYP2A6-transfected Huh7 cells. These results suggested that CYP2A6 might be related to the pathological process when HEV invaded host cells.

Published
2009

33. [Recombinant HEV caspid protein p239 specifically attached on HepG2 cells and blocked the infection of wild-type HEV on liver cells].

Author

Miao J, Zheng ZZ, He SZ, Liu PG, Wu XC, Sun YY, Tang M, Zhang J, and Xia NS

Subjects
Blotting, Western, Capsid Proteins genetics, Cell Line, Tumor, Fluorescent Antibody Technique, Hepatitis E virus genetics, Hepatitis E virus growth & development, Hepatocytes metabolism, Humans, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Capsid Proteins metabolism, Hepatitis E virus metabolism, Hepatocytes virology, Recombinant Proteins metabolism
Abstract

By using Western blot and immunofluorescence assays, the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature. Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells, indicating that p239 and HEV competed the same targeting site on these cells. These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.

Published
2007

Catalog

Books, media, physical & digital resources
See catalog results