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研究不同质量浓度的赤霉素（GA）及芸苔素内酯（BR）及二者交互作用对籽叶两用茶树光合特性及茶果产量的影响。以5年生的金茶1号为试验对象，采用不同质量浓度的GA(0、0.8、1.0、1.2、1.5 mg/L)、BR（0、0.3、0.5、0.8、1.0 mg/L）及二者交互处理（GA+BR 0 mg/L+0 mg/L、1.0 mg/L+0.5 mg/L、1.0 mg/L+0.8 mg/L、 1.2 mg/L+0.5 mg/L、 1.2 mg/L+0.8 mg/L）在茶果油脂转化期对茶树进行喷施,测定各处理茶树成熟期果实含水率，茶叶籽含油率，叶片含氮量、叶绿素含量、光合作用，以及成熟茶果的生物学特性和单株产量。结果表明：随着果实的成熟，茶果含水率逐渐降低，茶叶籽含油率先升高后降低（除对照），且同一时期喷施GA、BR及二者交互处理的茶果含水率均低于未喷施外源激素的茶果，茶叶籽含油率均高于未喷施外源激素的茶果；GA（1.2 mg/L）、BR(0.8 mg/L)及二者交互处理(GA 1.0 mg/L+BR 0.8 mg/L)的茶果成熟时含水率最低，分别为21.17%、25.75%、20.13%，茶叶籽含油率最高，成熟期的最高值分别为35.97%、33.52%、36.17%，单株产量最高，分别为1.91、1.91、1.95 kg；含氮量、叶绿素含量及光合作用随着果实的成熟呈现先升高后降低的趋势；成熟茶果的单株产量与成熟期的叶绿素含量、净光合速率、一籽质量、二籽质量及三籽质量呈极显著正相关（p

The stem tips of Zizania latifolia at different development stages were used as research materials. The expression stability of nine candidate reference genes (ACT1, H2B, UBI, EF-1α, GAPDH, β-actin, 60S, SKIP and AQP) were evaluated using qRT-PCR. The data were analyzed with GeNorm and NormFinder software. Present results indicated that the expression of ACT1 was stable and that it could be used as the optimal reference gene for studying the development stage of gall formation. ACT1 was selected as the reference gene to verify the expression level of the correlative genes in the gall formation stage of Z. latifolia. Our results were consistent with the previous transcriptome sequencing results. This study revealed that ACT1 was the classic reference gene for the analysis of correlative genes in all of the gall development stages of Z. latifolia. [ABSTRACT FROM AUTHOR]

The study showed that different ecological environment and cultivation system in various ecological regions of Zhejiang Province resulted in some different physiological characteristics of nitrogen nutrition and stress-resistance, especially in the aspect of NO3(-)-N and NH4+-N concentrations, between film-mulched and conventional flooded rice. Owing to the heat stress in Hangjiahu plain, the NO3(-)-N concentration of film-mulched rice decreased to some extent, but NH4+-N concentration increased markedly at tillering, jointing and booting stages, compared to conventional flooded rice. In Jinqu basin, the NO3(-)-N concentration of film-mulched rice at booting stage was higher, while the NH4+-N concentration in its roots was notably lower than those of conventional flooded rice, with NH4+-N concentration in its basal stems and leaves somewhat increased. Generally, the glutamine synthetase (GS) and nitrate reductase (NR) activities in film-mulched rice leaves were enhanced at booting stage, while malondiadehyde (MDA), soluble sugar (SS) and proline (Pro) concentrations had little changes. In conclusion, film-mulched cultivation was beneficial to the rice growth and its high yielding.